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Denaturing gradient gel electrophoresis (DGGE)-based monitoring of intestinal lactobacilli and bifidobacteria of pigs during a feeding trial
- Source :
- Archives of Animal Nutrition. 63:112-126
- Publication Year :
- 2009
- Publisher :
- Informa UK Limited, 2009.
-
Abstract
- The aim of this study was to determine the influence of different feeding strategies on the gut microbiota of organic growing-finishing pigs. A total of 76 pigs were allocated to four different dietary treatments (control, probiotics, maize silage and grass silage). Effects of the applied probiotic preparation on the composition of the intestinal and faecal microbiota were monitored. By using a DGGE (denaturing gradient gel electrophoresis)-based methodology, fingerprints of the intestinal microbiota were obtained. The total microbial DNA was isolated from faecal and colon samples and amplified with PCR using different primer sets to detect bifidobacteria and lactobacilli. PCR products were separated using DGGE and the resulting profiles were compared with the findings of the other dietary treatments. Bands were excised from the gels and sequenced for further identification. Particularly two different DGGE profiles of bifidobacteria were observed, while lactobacilli showed larger variety within the dietary treatments.
- Subjects :
- DNA, Bacterial
Microbial DNA
Swine
Silage
Gut flora
Microbiology
law.invention
Feces
Probiotic
fluids and secretions
law
Lactobacillus
Animals
Electrophoresis, Gel, Two-Dimensional
General Veterinary
biology
food and beverages
General Medicine
biology.organism_classification
Animal Feed
Gastrointestinal Contents
Diet
Intestines
Bifidobacteriaceae
Animal Nutritional Physiological Phenomena
Animal Science and Zoology
Bifidobacterium
Temperature gradient gel electrophoresis
Subjects
Details
- ISSN :
- 14772817 and 1745039X
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Archives of Animal Nutrition
- Accession number :
- edsair.doi.dedup.....e424ea33a4e41de8616e6f9d2ce242ff