1. Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice
- Author
-
Tatsushi Mutoh, Ryota Tochinai, Hiroaki Aono, Masayoshi Kuwahara, Yasuyuki Taki, and Tatsuya Ishikawa
- Subjects
cisterna magna ,clot distribution ,filament perforation ,mouse model ,subarachnoid hemorrhage ,Medicine (General) ,R5-920 - Abstract
Abstract The murine model of subarachnoid hemorrhage (SAH) is a valuable experimental tool for investigating molecular and cellular mechanisms, and the endovascular filament perforation technique can be used to simulate prominent pathophysiological features observed after human SAH; however, current validation methods for assessing an appropriate SAH model are limited. Here, we introduce a simple procedure for selecting a mouse model of diffuse SAH. SAH was induced in 24 mice using a standard filament perforation method. After confirming survival at 24 h, SAH was scored 0–1 based on T2*‐weighted images on whole‐brain magnetic resonance imaging (MRI) and visual surveillance of the cisterna magna (CM) through the dura mater. The CM‐based SAH grading correlated well with a reference parameter defined by extracted brain (r2 = 0.53, p
- Published
- 2024
- Full Text
- View/download PDF