Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice

Abstract The murine model of subarachnoid hemorrhage (SAH) is a valuable experimental tool for investigating molecular and cellular mechanisms, and the endovascular filament perforation technique can be used to simulate prominent pathophysiological features observed after human SAH; however, current validation methods for assessing an appropriate SAH model are limited. Here, we introduce a simple procedure for selecting a mouse model of diffuse SAH. SAH was induced in 24 mice using a standard filament perforation method. After confirming survival at 24 h, SAH was scored 0–1 based on T2*‐weighted images on whole‐brain magnetic resonance imaging (MRI) and visual surveillance of the cisterna magna (CM) through the dura mater. The CM‐based SAH grading correlated well with a reference parameter defined by extracted brain (r2 = 0.53, p