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Your search keyword '"Maeda, Eiki"' showing total 4 results
Start Over Author "Maeda, Eiki" Journal analytical chemistry
4 results on '"Maeda, Eiki"'

1. Carbohydrate--protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose

Author

Dang, Fuquan, Maeda, Eiki, Osafune, Tomo, Nakajima, Kazuki, Kakehi, Kazuaki, Ishikawa, Mitsuru, and Baba, Yoshinobu

Subjects
Polymethylmethacrylate -- Research, Polymethylmethacrylate -- Usage, Proteins -- Research, Carbohydrates -- Research, Chemistry
Abstract

We developed a novel method for rapid screening of carbohydrate--protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from [alpha] 1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC. 10.1021/acg02014c

Published
2009

4. Carbohydrate—Protein Interactions Investigated on Plastic Chips Statically Coated with Hydrophobically Modified Hydroxyethylcellulose.

Author

Fuquan Dang, Maeda, Eiki, Osafune, Tomo, Nakajlma, Kazuki, Kakehi, Kazuaki, Ishikawa, Mitsuru, and Baba, Yoshinobu

Subjects
*CARBOHYDRATES, *PROTEINS, *CHEMICAL reactions, *HYDROPHOBIC surfaces, *PHOSPHATES, *GLYCOPROTEINS
Abstract

We developed a novel method for rapid screening of carbohydrate—protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dyisamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from (il-add glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC. [ABSTRACT FROM AUTHOR]

Published
2009
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