Carbohydrate—Protein Interactions Investigated on Plastic Chips Statically Coated with Hydrophobically Modified Hydroxyethylcellulose.

We developed a novel method for rapid screening of carbohydrate—protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dyisamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from (il-add glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC. [ABSTRACT FROM AUTHOR]