1. A gel-scanning method for kinetic studies on an acetylcholinesterase isozyme
- Author
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Y.C. Chiu, R.K. Tripathi, and R.D. O'Brien
- Subjects
Erythrocytes ,Kinetics ,Biophysics ,Acetates ,Naphthalenes ,Biochemistry ,Esterase ,Isozyme ,Catalysis ,chemistry.chemical_compound ,Methods ,Animals ,Molecular Biology ,Cholinesterase ,Acrylamides ,Chromatography ,biology ,Elution ,Esterases ,Organothiophosphorus Compounds ,Cell Biology ,Electrophoresis, Disc ,Acetylcholinesterase ,Acetylcholine ,Staining ,Isoenzymes ,chemistry ,Spectrophotometry ,biology.protein ,Cattle ,Colorimetry ,Cholinesterase Inhibitors ,Gels ,Sulfur - Abstract
A gel-scanning technique involving gel staining and spectrophotometric scanning has been developed for quantitative assay of cholinesterase and other esterase isozymes. The kinetics of catalysis and inhibition can be directly determined without elution from the gel.
- Published
- 1972
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