A gel-scanning method for kinetic studies on an acetylcholinesterase isozyme

Authors :: Y.C. Chiu
R.K. Tripathi
R.D. O'Brien
Source :: Analytical Biochemistry. 45:480-487
Publication Year :: 1972
Publisher :: Elsevier BV, 1972.
Abstract: A gel-scanning technique involving gel staining and spectrophotometric scanning has been developed for quantitative assay of cholinesterase and other esterase isozymes. The kinetics of catalysis and inhibition can be directly determined without elution from the gel.