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We have developed a simple and convenient route to prepare fluorescent carbon dots with dual emission peaks respectively at 470 and 570 nm. The prepared dual-emission carbon dots can be used for ratiometric detection of Fe3+ ions in the range from 0 to 50 μmol·L-1 with 0.8 μmol·L-1 detection limit based on the fluorescence quenching at 570 nm. The quenched fluorescence induced by Fe3+ ions could be recovered by pyrophosphate. We further used the carbon dots-Fe3+ ions-pyrophosphate mixed system for ratiometric detection of acid phosphatase in the range from 0.08 to 6.75 μg·mL-1 with 0.01 μg·mL-1 detection limit. Image 1 • The carbon dots were directly prepared by carbonizing gallic acid and o-phenylenediamine in aqueous solutions. • The carbon dots exhibited dual emission peaks at 470 nm and 570 nm with excitation of 360 nm. • An obvious fluorescence quenching around 570 nm was observed with the addition of Fe3+ in acidic environments. • The GN-CDs can be used as a ratiometric probe for detection of Fe3+ ions and acid phosphatase. [ABSTRACT FROM AUTHOR]

DNA detection plays an important role in early diagnosis of genetic disease. The conventional detection methods of DNA are based on expensive equipment, which do not meet the demands of developing countries. Thus, we developed a colorimetric method, which could be observed with naked eye and used copper nanoclusters for cost-effective. Moreover, the target of this method is the DNA in Hepatitis B virus that is one of the most popular chronic viral infections in developing countries over the past years. Our method was sensitive and the limit of detection was 12 × 10 9 molecules. Three-base-pair mismatches target DNA was detected easily. These results revealed the favorable sensitivity and selectivity of this approach. Most importantly, our method may have potential applications in correct diagnosis of genetic disease and monitoring of gene therapy in the poverty-stricken areas. [ABSTRACT FROM AUTHOR]

A facile chemical synthetic route was developed to prepare near-infrared fluorescent trypsin-stabilized Ag nanoclusters (Try-Ag NCs). The fluorescence emission wavelength of the produced Try-Ag NCs is tunable by simple adjusting pH value of the synthesis system, and the Try-Ag NCs offer a symmetric fluorescent excitation and emission peak. The fluorescence of Try-Ag NCs remains constant in the presence of various ions and molecules, and it can be effectively quenched by 1,4-dihydronicotinamide adenine dinucleotide (NADH) instead of its oxidized forms nicotinamide adenine dinucleotide (NAD + ). This property enables the Try-Ag NCs to be a novel analytical platform to monitor biological reaction involved with NADH. In this work, the Try-Ag NCs was also applied to analyze ethanol based on the generation of NADH which was the product of NAD + and ethanol in the catalysis of alcohol dehydrogenase. And the proposed platform allowed ethanol to be determined in the range from 10 to 300 μmol/L with 5 μmol/L detection limit. [ABSTRACT FROM AUTHOR]

Highlights: [•] Cd2+ reacts with S2− to generate fluorescent CdS QDs with ATP. [•] ATP can be hydrolyzed by the enzymatic dephosphorylation of ALP. [•] Fluorescent CdS QDs could not be generated in the presence of ALP. [•] The analysis system was successfully applied to assay ATP and ALP. [ABSTRACT FROM AUTHOR]

To achieve the dual-channel (analog and digital) encoding, microbeads assembled with quantum dots (QDs) and element coding nanoparticles (ECNPs) have been prepared. Dual-spectra, including fluorescence generated from quantum dots (QDs) and laser induced breakdown spectrum obtained from the plasma of ECNPs, including AgO, MgO and ZnO nanoparticles, has been adopted to provide more encoding amounts and more accurate dual recognition for encoded microbeads in multiplexed utilization. The experimental results demonstrate that the single microbead can be decoded in two optical channels. Multiplexed analysis and contrast adsorption experiment of anti-IgG verified the availability and specificity of dual-channel-coded microbeads in bioanalysis. In gradient detection of anti-IgG, we obtained the linear concentration response to target biomolecules from 3.125 × 10 −10  M to 1 × 10 −8  M, and the limit of detection was calculated to be 2.91 × 10 −11  M. [ABSTRACT FROM AUTHOR]