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Facile synthesis of near infrared fluorescent trypsin-stabilized Ag nanoclusters with tunable emission for 1,4-dihydronicotinamide adenine dinucleotide and ethanol sensing.
- Source :
-
Analytica Chimica Acta . Jul2015, Vol. 886, p151-156. 6p. - Publication Year :
- 2015
-
Abstract
- A facile chemical synthetic route was developed to prepare near-infrared fluorescent trypsin-stabilized Ag nanoclusters (Try-Ag NCs). The fluorescence emission wavelength of the produced Try-Ag NCs is tunable by simple adjusting pH value of the synthesis system, and the Try-Ag NCs offer a symmetric fluorescent excitation and emission peak. The fluorescence of Try-Ag NCs remains constant in the presence of various ions and molecules, and it can be effectively quenched by 1,4-dihydronicotinamide adenine dinucleotide (NADH) instead of its oxidized forms nicotinamide adenine dinucleotide (NAD + ). This property enables the Try-Ag NCs to be a novel analytical platform to monitor biological reaction involved with NADH. In this work, the Try-Ag NCs was also applied to analyze ethanol based on the generation of NADH which was the product of NAD + and ethanol in the catalysis of alcohol dehydrogenase. And the proposed platform allowed ethanol to be determined in the range from 10 to 300 μmol/L with 5 μmol/L detection limit. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032670
- Volume :
- 886
- Database :
- Academic Search Index
- Journal :
- Analytica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 109159868
- Full Text :
- https://doi.org/10.1016/j.aca.2015.07.001