Search

It is critical to detect cellular secreted hydrogen peroxide (H 2 O 2) in situ for clinical diagnosis, biomedical research and cancer treatment. Herein, the electrochemical determination of H 2 O 2 released by cancer cells grown on the surface of carbon cloth supported NiCo-DH/AuPt micro-nano arrays to elevate the capability of in situ signal collection was achieved. NiCo-DH/AuPt @CC was successfully prepared using the cobalt based metal–organic framework (Co-MOF) as a presoma after in situ etching growth onto the CC and electrodeposition of gold and platinum nanoparticles (AuPt NPs). Under the optimal conditions, owing to the excellent catalytic efficiency of NiCo-DH and AuPt NPs, the designed sensor performs a relatively wider linear range to H 2 O 2 concentration from 10 μM to 22.08 mM, and the limit of detection is 0.145 μM. Accordingly, the as-prepared sensing system was also applied to determine H 2 O 2 secreted by living cells which grown on the surface of NiCo-DH/AuPt @CC with satisfactory consequences. In possession of the superior sensitivity, selectivity, reproducibility, the NiCo-DH/AuPt @CC is a luciferous platform for the real time detection of H 2 O 2 in the area of biomedical and clinical diagnosis. Image 1 • NiCo-DH's unique hollow triangular column structure was benefit for the adhesion of living cancer cells. • The detection distance between materials and H 2 O 2 could be shorten by cells directly adherent growth on the electrode. • The designed H 2 O 2 sensor exhibits a relatively wider linear current response with a low detection limit. [ABSTRACT FROM AUTHOR]

Herein, a three-dimensional graphene wall (GWs) and nano-Cu 2 O modified carbon fiber paper (CFP) electrode were used to develop a disposable and sensitive non-enzymatic glucose sensor. This sensing interface of GWs/Cu 2 O consists of an interlaced CFP on which intercrossed graphene walls (GWs) were vertically tethered in situ by radio frequency plasma enhanced chemical vapor deposition (RF-PECVD), and Cu 2 O nanoparticles (NPs) were evenly grew on the 3D GWs layer and skeleton through the complete thermal decomposition of copper acetate (Cu (CH 3 COO) 2) at high temperature. The CFP/GWs/Cu 2 O shows a large specific surface area and rich solution diffusion channels, which can expose more catalytic active sites without Nafion fixation film, thus greatly improving the electrocatalytic performance of this glucose sensor. The CFP/GWs/Cu 2 O sensor shows excellent catalytic performance to glucose with a linear detection range of 0.5 μM–5166 μM, LOD of 0.21 μM, and response time <4 s. This kind of disposable and sensitive electrode can capable of controlling uniform growth and accurate quantification, and has great development potential in the field of medical detection and the commercialization of wearable sensors. Image 1 • A disposable and sensitive non enzyme glucose sensor was developed by using GWs and nano Cu 2 O modified CFP electrode. • The staggered GWs were fixed vertically on the 3-D interlaced CFP by RF-PECVD. • The CFP/GWs/Cu 2 O shows a large specific surface area and rich solution diffusion channels. • Compared with the commercial glucose biosensor, the CFP/GWs/Cu 2 O electrode has excellent catalytic performance. [ABSTRACT FROM AUTHOR]