Flexible nickel–cobalt double hydroxides micro-nano arrays for cellular secreted hydrogen peroxide in-situ electrochemical detection.

It is critical to detect cellular secreted hydrogen peroxide (H 2 O 2) in situ for clinical diagnosis, biomedical research and cancer treatment. Herein, the electrochemical determination of H 2 O 2 released by cancer cells grown on the surface of carbon cloth supported NiCo-DH/AuPt micro-nano arrays to elevate the capability of in situ signal collection was achieved. NiCo-DH/AuPt @CC was successfully prepared using the cobalt based metal–organic framework (Co-MOF) as a presoma after in situ etching growth onto the CC and electrodeposition of gold and platinum nanoparticles (AuPt NPs). Under the optimal conditions, owing to the excellent catalytic efficiency of NiCo-DH and AuPt NPs, the designed sensor performs a relatively wider linear range to H 2 O 2 concentration from 10 μM to 22.08 mM, and the limit of detection is 0.145 μM. Accordingly, the as-prepared sensing system was also applied to determine H 2 O 2 secreted by living cells which grown on the surface of NiCo-DH/AuPt @CC with satisfactory consequences. In possession of the superior sensitivity, selectivity, reproducibility, the NiCo-DH/AuPt @CC is a luciferous platform for the real time detection of H 2 O 2 in the area of biomedical and clinical diagnosis. Image 1 • NiCo-DH's unique hollow triangular column structure was benefit for the adhesion of living cancer cells. • The detection distance between materials and H 2 O 2 could be shorten by cells directly adherent growth on the electrode. • The designed H 2 O 2 sensor exhibits a relatively wider linear current response with a low detection limit. [ABSTRACT FROM AUTHOR]