1. Microsatellite primers in red bayberry, Myrica rubra (Myricaceae)
- Author
-
Yingying Qiu, Sang-Nag Ahn, Xingjiang Qi, Xiliang Zheng, Xiaobo Xie, Liping Ke, Jinqing Chen, and Guanting Wu
- Subjects
Genetics ,Polymorphism, Genetic ,biology ,DNA, Plant ,Population structure ,Locus (genetics) ,Plant Science ,biology.organism_classification ,Polymerase Chain Reaction ,Myricaceae ,Myrica ,genomic DNA ,Species Specificity ,Genetic Loci ,Microsatellite ,Hybridization, Genetic ,Primer (molecular biology) ,Allele ,Ecology, Evolution, Behavior and Systematics ,Myrica rubra ,Alleles ,Genome, Plant ,DNA Primers ,Microsatellite Repeats - Abstract
PREMISE OF THE STUDY Microsatellite markers were developed in Myrica rubra to investigate potential hybridization events within or between M. rubra and its closely related species. METHODS AND RESULTS Using an ISSR-suppression PCR method, 12 primer pairs were temporarily developed with GSG(GT)(6) as the primer for enriching microsatellite sequences and the genomic DNA of M. rubra cv. 'Heijing' as template. The average allele number per locus was 4.9 within 26 individuals including two species, M. rubra and M. nana. Three pairs of primers produced species-specific alleles, and the other nine showed polymorphisms among 26 accessions. CONCLUSIONS Results indicated that the ISSR-suppression PCR method is suitable for developing microsatellite markers, especially for this species with little understanding of genomic information. The developed microsatellite markers provide a useful tool for further studies of population structure within or between M. rubra and M. nana or other closely related species.
- Published
- 2011