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Start Over Author "Nakamura, Rika" Search Limiters Full Text Journal allergology international
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2. Evaluation of the Luciferase Assay-Based In VitroElicitation Test for Serum IgE

Author

Nakamura, Ryosuke, Ishiwatari, Ayano, Higuchi, Masakazu, Uchida, Yoshimi, Nakamura, Rika, Kawakami, Hiroshi, Urisu, Atsuo, and Teshima, Reiko

Abstract

An in vitroelicitation test employing human high-affinity IgE receptor-expressing rat mast cell lines appears to be a useful method for measuring mast cell activation using a patient's IgE and an allergen; however, such cell lines are sensitive to human complements in the serum. We have recently developed a new luciferase-reporting mast cell line (RS-ATL8) to detect IgE crosslinking-induced luciferase expression (EXiLE) with relatively low quantities of serum IgE.

Published
2012
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