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The changing landscape of higher education orchestrated by the growing use of e-learning presents significant opportunities and threats to higher education institutions in developing countries like Nigeria. However, e-learning is not widely adopted by Nigerian institutions due to contextual and individual barriers. This study aims to advance our understanding of the determinants of e-learning adoption by reconceptualising e-learning adoption barriers from an entrepreneurial and strategic perspective. Using Partial Least Square approach to Structural Equation Modelling (PLS-SEM) to analyse data collected from 305 respondents, the findings of the study demonstrate that entrepreneurial orientation and strategic planning significantly influence e-learning adoption. The findings of the study advance our understanding of e-learning adoption barriers and also provide new insights to managers of higher education institutions with regards to e-learning adoption. Key words: E-learning, e-learning adoption, entrepreneurial orientation, strategic planning, higher education institutions.