1. Comparison of a fluorometric assay kit with high-performance liquid chromatography for the assessment of serum retinol concentration
- Author
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El Haloui Noureddine, Azlaf Mehdi, El Hamdouchi Asmaa, Aguenaou Hassan, Benjeddou Kaoutar, Aglago Kouassivi Elom, El Menchawy Imane, and El Kari Khalid
- Subjects
Vitamin ,Male ,Rural Population ,Serum retinol ,High-performance liquid chromatography ,Vial ,Sensitivity and Specificity ,chemistry.chemical_compound ,Medicine ,Humans ,Sample preparation ,Fluorometry ,Longitudinal Studies ,Child ,Vitamin A ,Chromatography, High Pressure Liquid ,Chromatography ,business.industry ,Vitamin A Deficiency ,Retinol ,Reproducibility of Results ,General Medicine ,Articles ,Morocco ,chemistry ,HPLC, ICHECK Fluoro, serum retinol, test kit, vitamin A status ,Female ,business ,Rural population - Abstract
Background : Although high-performance liquid chromatography (HPLC) is the commonly used method for the analysis of retinol in biological samples, simple and rapid test kits are available. Objectives : This study compared a rapid test kit (ICHECK Fluoro®) to HPLC for the assessment of serum retinol concentrations. Methods : For the analysis by HPLC, sample preparation included standard deproteinization and extraction phases. The analysis by ICHECK was performed by injecting serum into IEX reagent vials (n=89) and mixing manually for separation. After precipitation of the proteins, the vial was introduced into the chamber of the ICHECK Fluoro and analysed at 0 min (ICHECK0min) and 15 min later (ICHECK15min). Bland and Altman approach was applied to test the agreement between HPLC and ICHECK. Results : Mean HPLC, ICHECK0min and ICHECK15min values were 421.2±106.0 μg/L, 423.1±118.3 μg/L and 413.2±107.6 μg/L, respectively. Retinol concentrations significantly decreased in the IEX solution over time (p
- Published
- 2015