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Your search keyword '"Hironobu Fujita"' showing total 3 results
Start Over Author "Hironobu Fujita" Journal acs synthetic biology
3 results on '"Hironobu Fujita"'

1. Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation

Author

Hironobu Fujita, Ayane Osaku, Yuto Sakane, Koki Yoshida, Kayoko Yamada, Seia Nara, Takahito Mukai, and Masayuki Su’etsugu

Subjects
DNA, Bacterial, Escherichia coli, Biomedical Engineering, Synthetic Biology, DNA, General Medicine, Chromosomes, Bacterial, Biochemistry, Genetics and Molecular Biology (miscellaneous), Chromosomes, Genome, Bacterial
Abstract

The physical stability of bacterial chromosomes is important for their in vitro manipulation, while genetic stability is important in vivo. However, extracted naked chromosomes in the open circular form are fragile due to nicks and gaps. Using a nick/gap repair and negative supercoiling reaction (named SCR), we first achieved the negative supercoiling of the whole genomes extracted from

Published
2022
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2. Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli

Author

Tatsuya Yoneji, Hironobu Fujita, Masayuki Su'etsugu, Kayoko Yamada, Takahito Mukai, and Seia Nara

Subjects
0106 biological sciences, Genetics, 0303 health sciences, Bacterial artificial chromosome, Bacterial conjugation, Biomedical Engineering, General Medicine, Biology, medicine.disease_cause, biology.organism_classification, 01 natural sciences, Biochemistry, Genetics and Molecular Biology (miscellaneous), 03 medical and health sciences, chemistry.chemical_compound, Plasmid, chemistry, 010608 biotechnology, medicine, Site-specific recombination, Escherichia coli, DNA, Bacteria, 030304 developmental biology
Abstract

Although Escherichia coli has been a popular tool for plasmid construction, this bacterium was believed to be “unsuitable” for constructing a large plasmid whose size exceeds 500 kilobases. We assu...

Published
2020
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3. Overcoming the Challenges of Megabase-Sized Plasmid Construction in

Author

Takahito, Mukai, Tatsuya, Yoneji, Kayoko, Yamada, Hironobu, Fujita, Seia, Nara, and Masayuki, Su'etsugu

Subjects
DNA Replication, Recombination, Genetic, Chromosomes, Artificial, Bacterial, Chloramphenicol, Genetic Vectors, Escherichia coli, Genetic Engineering, Vibrio
Abstract

Although

Published
2020

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