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Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli
- Source :
- ACS Synthetic Biology. 9:1315-1327
- Publication Year :
- 2020
- Publisher :
- American Chemical Society (ACS), 2020.
-
Abstract
- Although Escherichia coli has been a popular tool for plasmid construction, this bacterium was believed to be “unsuitable” for constructing a large plasmid whose size exceeds 500 kilobases. We assu...
- Subjects :
- 0106 biological sciences
Genetics
0303 health sciences
Bacterial artificial chromosome
Bacterial conjugation
Biomedical Engineering
General Medicine
Biology
medicine.disease_cause
biology.organism_classification
01 natural sciences
Biochemistry, Genetics and Molecular Biology (miscellaneous)
03 medical and health sciences
chemistry.chemical_compound
Plasmid
chemistry
010608 biotechnology
medicine
Site-specific recombination
Escherichia coli
DNA
Bacteria
030304 developmental biology
Subjects
Details
- ISSN :
- 21615063
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- ACS Synthetic Biology
- Accession number :
- edsair.doi...........fc97dd5a6aa5dbcd87682f32c3259328
- Full Text :
- https://doi.org/10.1021/acssynbio.0c00008