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The purpose of the current work was to study the winter wheat varieties artificially infected with the North Caucasian population of brown rust to identify a damage degree. The study was carried out in two stages on two sets (8 and 10) of varieties with different level of resistance to the pathogen. The trials have established a high damage degree of rust on susceptible varieties, which resulted in productivity decrease from 16.3 to 32.2%. Among the varieties with the maximum damage degree, there was identified the most resistant variety ‘Tarasovskaya 29’ which, when completely (100%) damaged by the disease, showed a minimum productivity decrease, no more than 10.6%. The varieties being poorly susceptible to brown leaf rust reduced the productivity from 11.2% to 20.7%. The smaller indices of productivity decrease among this group were shown by the variety ‘Spartak’. Among the five varieties that showed moderate leaf rust damage, there were different degrees of productivity decrease. The variety ‘Donskaya Yubileynaya’ artificially infected with brown rust, showed a minimal productivity decrease and its structural elements, and it could be identified as the leaf rust resistant variety like the variety ‘Tarasovskaya 29’. When predicting the brown leaf rust epiphytoty, the susceptible and half-resistant wheat varieties require obligatory protection with fungicides.

In order to obtain high yields of winter durum wheat, it is necessary not only to use advanced agricultural technologies, but also to sow varieties resistant to major diseases. One of these diseases that cause great harm to grain crops is Septoria leaf blotch. The current paper has presented the study results of collection material of Russian and foreign winter durum wheat according to the efficiency of the Septoria leaf blotch resistance gene Stb2 in the North Caucasus region. The purpose of the current study was to identify the Septoria leaf blotch resistance gene Stb2 in the collection material of winter durum wheat. The study was carried out in 2017–2018 in the laboratory of marker breeding of the FSBSI “ARC “Donskoy” in Zernograd. In the course of the study there have been applied such methods as the isolation of genomic DNA by the CTAB-method, PCR-analysis with the molecular SSR marker WMS533, electrophoresis on 2% agarose gel, analysis of electrophoresis gels in the Bio-Rad Image Lab 6.0 software, general data analysis by Microsoft Excel. The study has shown that 29 samples out of 185 analyzed ones had the Septoria leaf blotch resistance gene Stb2, they are the lines L3557 h42, L3182 h7, L3335 h29, L3336 h43, L3408 h218 (Russia, FSBSI “Research Center of Plants named after P. P. Lukyanenko”), K-61620 (Russia), K-54455, DF 917 (Romania), K-61924, K-59179, K-51863, K-61869 (Moldova), K-63871 (Turkey), Kharkovskaya 909 (Ukraine), MVTD-15-99 (Hungary), 2196-1/04, 922/93, 589/13, 655/13, 683/13, 145/14, 322/14, 484/14, Gordeiform 1424/82 (Russia, FSBSI “ARC “Donskoy”), Beliy Parus (Ukraine), Belgorodskaya Yantarnaya (LLC “Sativa” in Belgorod, Russia), IR named after V. Ya. Yuriev (Kharkov, Ukraine)), Zhivitsa (Russia, Prikumskaya OSS), Pandur (Romania) and Elidur (Romania). There have been recommended using the identified samples of winter durum wheat with the Septoria leaf blotch resistance gene Stb2 in breeding programs.

The current paper presents the 3-year assessment results of domestic and foreign winter barley varieties and lines for smut resistance. The smut pathogen in the Rostov region causes one of the most harmful diseases, which, in addition to direct crop losses (head destruction), causes the so-called hidden losses, which can several times exceed direct ones. An effective way to counteract the disease is to cultivate resistant varieties, and farmers need the reliable sources of resistance to the pathogen. The immunological properties of winter barley varieties were studied in an infectious nursery (2016–2018). Seed infection with spores was carried out by S. Dimitrov’s method (1968) using a nutrient medium. Inoculated seeds were sown at the end of the optimal time. There was estimated a number of infected stems. The variety “Dobrynya 3” was used as a susceptible test-variety, which percentage of infection throughout the years ranged from 20.6 to 71.8%. There were studied 115 domestic and foreign varieties and lines. Among 69 domestic commercial varieties and breeding lines, 11 ones (15.9%) showed good resistance, 21 ones (30.4%) showed weak susceptibility, 30 ones (43.5%) possessed average susceptibility and seven varieties (10.1%) were severely affected by the pathogen. Among foreign varieties, resistance to barley smut has been established in seven German varieties, in three French varieties, in two Swiss varieties, one Czech variety and one Ukrainian variety. The varieties “Rocca”, “Nixe” (Germany), “Metelitsa” (Ukraine) are classified as the best ones in resistance to the pathogen during the artificial infection. The French variety “18513 ЕН11” had no affected plants during the study and was identified as an immune variety. The identified sources of winter barley resistance to smut are recommended for use in breeding programs for immunity.

On the fields with infectious backgrounds more than one thousands of varieties and samples of collection and breeding material of winter wheat are annually estimated for resistance to six diseases (brown and yellow rust, leaf blotch, powdery mildew, loose and kernel smut). Among the collection material there have been identified the samples tolerant and resistant to all studied diseases as well as the samples with complex resistance to 3–6 pathogens (e. g. “Maris Kinsman”, “Tucan”, “Liwilla”, “KS93V76”, “PL 178383” and others). Such varieties of the Inter-Station Variety Testing (ISVT) from Russia, Ukraine, Germany, France as “Etana”, “Kurs”, “Rotaks”, “Solokha”, “Shestopalovka” cannot be artificially infected with the diseases. There has been identified a small percentage among the present varieties and collection samples tolerant to such diseases as powdery mildew, leaf blotch and kernel smut. The winter soft wheat varieties developed in the FSBSI “ARC “Donskoy” both widely cultivated in production, and introduced for study in the SVT in recent years, are characterized by a high resistance to 2–4 studied diseases. These are the varieties “Tanais”, “Rostovchanka 7”, “Aksiniya”, “Nakhodka” which are recommended to plant after fallow forecrops and the varieties “Krasa Dona”, “Ermak”, “Donskoy Syurpriz”, “Don 107”, “Izyuminka”, “Asket”, “Lidiya” which are recommended to plant after non-fallow forecrops. The identified resistant varieties are recommended for wide cultivation, and the sources of resistance to a complex of the main harmful diseases on the Nizhny Don can be used in the breeding programs on immunity.