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Type 2 diabetes (T2D) is a heterogeneous disease that develops through diverse pathophysiological processes1,2and molecular mechanisms that are often specific to cell type3,4. Here, to characterize the genetic contribution to these processes across ancestry groups, we aggregate genome-wide association study data from 2,535,601 individuals (39.7% not of European ancestry), including 428,452 cases of T2D. We identify 1,289 independent association signals at genome-wide significance (P< 5 × 10−8) that map to 611 loci, of which 145 loci are, to our knowledge, previously unreported. We define eight non-overlapping clusters of T2D signals that are characterized by distinct profiles of cardiometabolic trait associations. These clusters are differentially enriched for cell-type-specific regions of open chromatin, including pancreatic islets, adipocytes, endothelial cells and enteroendocrine cells. We build cluster-specific partitioned polygenic scores5in a further 279,552 individuals of diverse ancestry, including 30,288 cases of T2D, and test their association with T2D-related vascular outcomes. Cluster-specific partitioned polygenic scores are associated with coronary artery disease, peripheral artery disease and end-stage diabetic nephropathy across ancestry groups, highlighting the importance of obesity-related processes in the development of vascular outcomes. Our findings show the value of integrating multi-ancestry genome-wide association study data with single-cell epigenomics to disentangle the aetiological heterogeneity that drives the development and progression of T2D. This might offer a route to optimize global access to genetically informed diabetes care.

Type 2 diabetes (T2D) shows heterogeneous body mass index (BMI) sensitivity. Here, we performed stratification based on BMI to optimize predictions for BMI-related diseases. We obtained BMI-stratified datasets using data from more than 195,000 individuals (nT2D= 55,284) from BioBank Japan (BBJ) and UK Biobank. T2D heritability in the low-BMI group was greater than that in the high-BMI group. Polygenic predictions of T2D toward low-BMI targets had pseudo-R2values that were more than 22% higher than BMI-unstratified targets. Polygenic risk scores (PRSs) from low-BMI discovery outperformed PRSs from high BMI, while PRSs from BMI-unstratified discovery performed best. Pathway-specific PRSs demonstrated the biological contributions of pathogenic pathways. Low-BMI T2D cases showed higher rates of neuropathy and retinopathy. Combining BMI stratification and a method integrating cross-population effects, T2D predictions showed greater than 37% improvements over unstratified-matched-population prediction. We replicated findings in the Tohoku Medical Megabank (n= 26,000) and the second BBJ cohort (n= 33,096). Our findings suggest that target stratification based on existing traits can improve the polygenic prediction of heterogeneous diseases.

The object of this paper is to develop numerical procedures for creep-fatigue-oxidation life prediction and damage assessments based on engineering damage approach, in response to high-reliability and long-life design requirement that supports low-carbon and new-energy technologies. In order to achieve the prediction results in terms of cycle-dependent stress–strain responses, crack initiation life prediction and multi-damage evolutions, the generalized creep-fatigue loading conditions including tension-hold-only, compression-hold-only and tension-compression-hold-both, which are abbreviated as CP, PC, and CC types, are conducted for IN 718 at 650 °C. Results show that creep-fatigue deformation behaviors are well depicted through the evolutions of hysteresis loops, cyclic softening curves and stress responses. With incorporating into oxidation damage especially under long-life conditions, both error band and probability density function for life prediction are quantitatively improved. In addition, the cycle-dependent roles in multi-damage evolutions are clearly observed in a set of radar graphs, where fatigue, creep and oxidation damage are manifested as different evolutionary features. Finally, the technical route in the transition from deterministic to probabilistic multi-damage assessments is discussed based on the established creep-fatigue-oxidation diagram.

According to quantum chromodynamics, vacuum is not an empty space, because it is filled with quark–antiquark pairs. The pair has the same quantum numbers as the vacuum and forms a condensate because the strong interaction of the quantum chromodynamics is too strong to leave the vacuum empty. This quark–antiquark condensation, the chiral condensate, breaks the chiral symmetry of the vacuum. The expectation value of the chiral condensate is an order parameter of the chiral symmetry, which is expected to decrease at high temperatures or high matter densities where the chiral symmetry is partially restored. Head-on collisions of nuclei at ultra-relativistic energies have explored the high-temperature regime, but experiments at high densities are rare. Here we measure the spectrum of pionic 121Sn atoms and study the interaction between the pion and the nucleus. We find that the expectation value of the chiral condensate is reduced at finite density compared to the value in vacuum. The reduction is linearly extrapolated to the nuclear saturation density and indicates that the chiral symmetry is partially restored due to the extremely high density of the nucleus.

Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge1–5. Here we conducted a genome-wide association study (GWAS) involving 2,393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3,289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2associated with the risk allele in these younger patients. DOCK2expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n= 61 individuals) identified cell-type-specific downregulation of DOCK2and a COVID-19-specific decreasing effect of the risk allele on DOCK2expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target.

We assembled an ancestrally diverse collection of genome-wide association studies (GWAS) of type 2 diabetes (T2D) in 180,834 affected individuals and 1,159,055 controls (48.9% non-European descent) through the Diabetes Meta-Analysis of Trans-Ethnic association studies (DIAMANTE) Consortium. Multi-ancestry GWAS meta-analysis identified 237 loci attaining stringent genome-wide significance (P< 5 × 10−9), which were delineated to 338 distinct association signals. Fine-mapping of these signals was enhanced by the increased sample size and expanded population diversity of the multi-ancestry meta-analysis, which localized 54.4% of T2D associations to a single variant with >50% posterior probability. This improved fine-mapping enabled systematic assessment of candidate causal genes and molecular mechanisms through which T2D associations are mediated, laying the foundations for functional investigations. Multi-ancestry genetic risk scores enhanced transferability of T2D prediction across diverse populations. Our study provides a step toward more effective clinical translation of T2D GWAS to improve global health for all, irrespective of genetic background.

Current genome-wide association studies do not yet capture sufficient diversity in populations and scope of phenotypes. To expand an atlas of genetic associations in non-European populations, we conducted 220 deep-phenotype genome-wide association studies (diseases, biomarkers and medication usage) in BioBank Japan (n= 179,000), by incorporating past medical history and text-mining of electronic medical records. Meta-analyses with the UK Biobank and FinnGen (ntotal= 628,000) identified ~5,000 new loci, which improved the resolution of the genomic map of human traits. This atlas elucidated the landscape of pleiotropy as represented by the major histocompatibility complex locus, where we conducted HLA fine-mapping. Finally, we performed statistical decomposition of matrices of phenome-wide summary statistics, and identified latent genetic components, which pinpointed responsible variants and biological mechanisms underlying current disease classifications across populations. The decomposed components enabled genetically informed subtyping of similar diseases (for example, allergic diseases). Our study suggests a potential avenue for hypothesis-free re-investigation of human diseases through genetics.

Molecular aggregation structure and mechanical as well as thermal properties of novel well-defined multiblock copolymers consisting of crystalline syndiotactic polystyrene (sPS) and rubbery hydrogenated polyisoprene (hPIp) were investigated. The morphology and crystalline ordered structure of the multiblock copolymer films prepared by solvent casting from 1,2-dichlorobenzene solution depended on the volume fraction of sPS (VFsPS) and number of blocks. The multiblock copolymer films exhibited ordered morphology with low crystallinity. The crystallinity of the sPS reduced with decreasing the VFsPS. The pentablock copolymer produced more ordered morphology and less crystallinity than the triblock copolymers. The anisotropic orientation and mechanical stability of the δ form sPS crystals in the spherical sPS domains during uniaxial stretching were demonstrated. Tensile testing and dynamic mechanical analysis indicated that these multiblock copolymer films with appropriate sPS fraction are strong, tough, and elastic and thus could be potential candidates for a new type of thermoplastic elastomer with discrete crystalline hard domains.

The overwhelming majority of participants in current genetic studies are of European ancestry. To elucidate disease biology in the East Asian population, we conducted a genome-wide association study (GWAS) with 212,453 Japanese individuals across 42 diseases. We detected 320 independent signals in 276 loci for 27 diseases, with 25 novel loci (P< 9.58 × 10−9). East Asian–specific missense variants were identified as candidate causal variants for three novel loci, and we successfully replicated two of them by analyzing independent Japanese cohorts; p.R220W of ATG16L2(associated with coronary artery disease) and p.V326A of POT1(associated with lung cancer). We further investigated enrichment of heritability within 2,868 annotations of genome-wide transcription factor occupancy, and identified 378 significant enrichments across nine diseases (false discovery rate < 0.05) (for example, NKX3-1for prostate cancer). This large-scale GWAS in a Japanese population provides insights into the etiology of complex diseases and highlights the importance of performing GWAS in non-European populations.

Meta-analyses of genome-wide association studies (GWAS) have identified more than 240 loci that are associated with type 2 diabetes (T2D)1,2; however, most of these loci have been identified in analyses of individuals with European ancestry. Here, to examine T2D risk in East Asian individuals, we carried out a meta-analysis of GWAS data from 77,418 individuals with T2D and 356,122 healthy control individuals. In the main analysis, we identified 301 distinct association signals at 183 loci, and across T2D association models with and without consideration of body mass index and sex, we identified 61 loci that are newly implicated in predisposition to T2D. Common variants associated with T2D in both East Asian and European populations exhibited strongly correlated effect sizes. Previously undescribed associations include signals in or near GDAP1, PTF1A, SIX3, ALDH2, a microRNA cluster, and genes that affect the differentiation of muscle and adipose cells3. At another locus, expression quantitative trait loci at two overlapping T2D signals affect two genes—NKX6-3and ANK1—in different tissues4–6. Association studies in diverse populations identify additional loci and elucidate disease-associated genes, biology, and pathways.

ABSTRACTThe basidiomycetous yeast Pseudozyma antarcticaGB-4(0) esterase (PaE) is a promising candidate for accelerating degradation of used biodegradable plastics (BPs). To increase safety and reduce costs associated with the use of PaE, we constructed a self-cloning strain with high-PaE productivity. A Lys12 gene (PaLYS12)-deleted lysine auxotroph strain GB4-(0)-L1 was obtained from GB-4(0) by ultraviolet mutagenesis and nystatin enrichment. Subsequently, the PaE gene (PaCLE1) expression cassette consisting of GB-4(0)-derived PaCLE1, under the control of a xylose-inducible xylanase promoter with PaLYS12, was randomly introduced into the GB4-(0)-L1 genome. A PaE high-producing strain, PGB474, was selected from among the transformants by high throughput double-screening based on its ability to degrade emulsified polybutylene succinate-co-adipate. Quantitative PCR revealed that four copies of the PaE gene expression cassette were introduced into the PGB474 genome. PGB474 produced 2.0 g/L of PaE by xylose-fed-batch cultivation using a 3-L jar fermentor for 72 h.

To perform detailed fine-mapping of the major-histocompatibility-complex region, we conducted next-generation sequencing (NGS)-based typing of the 33 human leukocyte antigen (HLA) genes in 1,120 individuals of Japanese ancestry, providing a high-resolution allele catalog and linkage-disequilibrium structure of both classical and nonclassical HLA genes. Together with population-specific deep-whole-genome-sequencing data (n= 1,276), we conducted NGS-based HLA, single-nucleotide-variant and indel imputation of large-scale genome-wide-association-study data from 166,190 Japanese individuals. A phenome-wide association study assessing 106 clinical phenotypes identified abundant, significant genotype–phenotype associations across 52 phenotypes. Fine-mapping highlighted multiple association patterns conferring independent risks from classical HLA genes. Region-wide heritability estimates and genetic-correlation network analysis elucidated the polygenic architecture shared across the phenotypes.

To understand the genetics of type 2 diabetes in people of Japanese ancestry, we conducted A meta-analysis of four genome-wide association studies (GWAS; 36,614 cases and 155,150 controls of Japanese ancestry). We identified 88 type 2 diabetes–associated loci (P< 5.0 × 10−8) with 115 independent signals (P< 5.0 × 10−6), of which 28 loci with 30 signals were novel. Twenty-eight missense variants were in linkage disequilibrium (r2> 0.6) with the lead variants. Among the 28 missense variants, three previously unreported variants had distinct minor allele frequency (MAF) spectra between people of Japanese and European ancestry (MAFJPN> 0.05 versus MAFEUR< 0.01), including missense variants in genes related to pancreatic acinar cells (GP2) and insulin secretion (GLP1R). Transethnic comparisons of the molecular pathways identified from the GWAS results highlight both ethnically shared and heterogeneous effects of a series of pathways on type 2 diabetes (for example, monogenic diabetes and beta cells).

Modified 9Cr-1Mo steel is a heat-resistant steel developed for a steam generator in a FBR (Fast Breeder Reactor) and it has been applied to various thermal power plants. Recently, it was found that the fatigue limits did not appear up to 108 cycles at temperatures higher than 500oC. The reason for the decrease of the fatigue life was attributed to the change of the initially designed microstructure of the alloy. The initially dispersed fine lath martensitic texture disappeared at temperatures higher than 500°C, when the magnitude of the applied stress exceeded a certain critical value. In order to explicate the dominant factors of the change quantitatively, the change of the microstructure and the strength of the alloy were continuously observed by applying an intermittent fatigue and creep tests at elevated temperatures and EBSD analysis. It was found that there was a critical stress which caused the microstructure change at each test temperature higher than 500°C, and the activation energy of the change was determined as a function of temperature and the applied tensile stress. The dominant factor of the micro structure change was the stress-induced acceleration of the atomic diffusion of the component element of the alloy.

The degradation process of the micro texture of Ni-base superalloys was observed under fatigue and creep-fatigue loading conditions at elevated temperatures higher than 700oC by applying electron back-scatter diffraction (EBSD) analyses. The local distribution of the crystallinity of a grain and a grain boundary was defined quantitatively by analyzing the Kikuchi pattern obtained from each electron-beam-irradiated area with a diameter of 50 nm. The calculated image quality value was used for the analysis. It was found that the crystallinity of grain boundaries degraded seriously under creep-fatigue loading conditions due to the acceleration of anisotropic strain-induced diffusion of component elements. Since the initial finely-controlled strengthened micro texture disappeared due to the anisotropic diffusion of component elements, this degradation was found to cause the drastic decrease of the strength of grains and grain boundaries and thus, lifetime of the material. The decrease of the strength of both grains and grain boundaries was measured by micro tensile test system in a scanning electron microscope by making a small sample from bulk specimen using focused ion beam. The strength of a grain and a grain boundary varied drastically depending on their crystallinity, in other words, the image quality value.

Brown fat dissipates energy as heat and protects against obesity. Here, we identified nuclear factor I-A (NFIA) as a transcriptional regulator of brown fat by a genome-wide open chromatin analysis of murine brown and white fat followed by motif analysis of brown-fat-specific open chromatin regions. NFIA and the master transcriptional regulator of adipogenesis, PPARγ, co-localize at the brown-fat-specific enhancers. Moreover, the binding of NFIA precedes and facilitates the binding of PPARγ, leading to increased chromatin accessibility and active transcription. Introduction of NFIA into myoblasts results in brown adipocyte differentiation. Conversely, the brown fat of NFIA-knockout mice displays impaired expression of the brown-fat-specific genes and reciprocal elevation of muscle genes. Finally, expression of NFIA and the brown-fat-specific genes is positively correlated in human brown fat. These results indicate that NFIA activates the cell-type-specific enhancers and facilitates the binding of PPARγ to control the brown fat gene program.

We have established a new transgenesis protocol based on CRISPR-Cas9, “New and Easy XenopusTransgenesis (NEXTrans),” and identified a novel safe harbor site in African clawed frogs, Xenopus laevis. We describe steps in detail for the construction of NEXTransplasmid and guide RNA, CRISPR-Cas9-mediated NEXTransplasmid integration into the locus, and its validation by genomic PCR. This improved strategy allows us to simply generate transgenic animals that stably express the transgene.

A surface marker that distinctly identifies cardiac progenitors (CPs) is essential for the robust isolation of these cells, circumventing the necessity of genetic modification. Here, we demonstrate that a Glycosylphosphatidylinositol-anchor containing neurotrophic factor receptor, Glial cell line-derived neurotrophic factor receptor alpha 2 (Gfra2), specifically marks CPs. GFRA2 expression facilitates the isolation of CPs by fluorescence activated cell sorting from differentiating mouse and human pluripotent stem cells. Gfra2mutants reveal an important role for GFRA2 in cardiomyocyte differentiation and development both in vitro and in vivo. Mechanistically, the cardiac GFRA2 signaling pathway is distinct from the canonical pathway dependent on the RET tyrosine kinase and its established ligands. Collectively, our findings establish a platform for investigating the biology of CPs as a foundation for future development of CP transplantation for treating heart failure.

Programmable nucleases enable engineering of the genome by utilizing endogenous DNA double-strand break (DSB) repair pathways. Although homologous recombination (HR)-mediated gene knock-in is well established, it cannot necessarily be applied in every cell type and organism because of variable HR frequencies. We recently reported an alternative method of gene knock-in, named the PITCh (Precise Integration into Target Chromosome) system, assisted by microhomology-mediated end-joining (MMEJ). MMEJ harnesses independent machinery from HR, and it requires an extremely short homologous sequence (5–25 bp) for DSB repair, resulting in precise gene knock-in with a more easily constructed donor vector. Here we describe a streamlined protocol for PITCh knock-in, including the design and construction of the PITCh vectors, and their delivery to either human cell lines by transfection or to frog embryos by microinjection. The construction of the PITCh vectors requires only a few days, and the entire process takes ∼1.5 months to establish knocked-in cells or ∼1 week from injection to early genotyping in frog embryos.

The 2007 to 2009 financial crises also known as 'global financial crisis' has shown the importance of contagion and interconnectedness among financial nodes. This trickles down the crisis effect to small nodes and whole system becomes fragile. We analysed selected EU members banking system network in terms of their pre- and post-crisis status. By using the matrix of bilateral obligations and their capital levels, conducted stress test simulations for both periods. Different levels of shocks entail most aspects of stress tests in terms of identifying networks strength and interconnectedness. Short-terms to long-term clearing scenarios are used to identify strong and weak nodes within system during both time frames. Default probabilities for different levels of shocks shows realistic picture of the banking systems, as they received huge bailouts and financing from governments and regulators. Default probabilities are used to identify the level of difficulty faced by particular institution or whole system.

While the concept of Cloud Radio Access Networks (C-RANs) presents a promising solution to provide required quality of service (QoS) for the future network environment, i.e., more than 10 Gbps capacity, less than 1 ms latency, and connectivity for numerous devices, it is still susceptible to quality of experience (QoE) problems. Until now, only a few researchers considered the design and operation of C-RANs based on QoE. In this article we describe our envisioned C-RAN based on passive optical networks (PONs) exploiting power over fiber (PoF), which can be installed with low installation cost and is capable of providing communication services without external power supply for remote radio head (RRH), and describe QoE requirement on the envisioned network. For all users in the envisioned network to satisfy their QoE, effective network design and operation approaches are then presented. Our proposed design and operation approaches demonstrate how to construct the envisioned network, i.e. the numbers of RRHs and optical line terminals (OLTs), and sleep scheduling of RRHs for an energy-efficient optical power transmission.

Fiber Wireless (FiWi) access networks is widely used to connect users to the core network. The FiWi access networks consist of the edge router, the Passive Optical Networks (PONs), and the wireless networks. In this environment, the edge router, which is the terminal to the core network, can have heavy congestion. To cope with this problem, we propose a Multi Point Control Protocol (MPCP)-based centralized rate control method to centrally control the traffic rate at each Home GateWay (HGW) so as to avoid congestion at the edge router. In the proposed scheme, the transmission delay due to rate control overhead can be mitigated. The performance of the proposed scheme is verified by numerical analysis and computer simulations.

The local thermal deformation of the chips mounted by area-arrayed fine bumps has increased drastically because of the decrease of the flexural rigidity of the thinned chips. In this paper, the dominant structural factors of the local residual stress in a silicon chip are investigated quantitatively based on the measurement of the local residual stress in a chip using stress sensor chips. The piezoresistive strain gauges were embedded in the sensor chips. The length of each gauge was 2 µm and a unit cell consisted of four gauges with different crystallographic directions. This alignment of strain gauges enables to measure the tensor component of three-dimensional stress fields separately. Test flip chip substrates were made by silicon chip on which the area-arrayed tin/copper bumps were electroplated. The width of a bump was fixed at 200 µm and the bump pitch was varied from 400 µm to 1,000 µm. The measured amplitude of the residual stress increased from about 30 MPa to 250 MPa. It was confirmed that both the material constant of underfill and the alignment structure of fine bumps are the dominant factors of the local deformation and stress of a silicon chip mounted on area-arrayed metallic bumps.

The relationship between the electrical properties and crystallographic quality (crystallinity) of electroplated copper thin-film interconnections was investigated. The crystallinity of the grains and grain boundaries of the interconnections was evaluated on the basis of the image quality (IQ) value obtained by electron back-scatter diffraction (EBSD) analysis. The electrical properties of the interconnections vary markedly depending on their crystallinity. The crystallinity also changed markedly as functions of electroplating conditions and the annealing temperature after electroplating. Although the electro migration (EM) resistance of the annealed interconnection was improved, stress-induced migration (SM) was activated by a high residual stress after annealing. To improve electrical reliability without heat treatment after electroplating, the effects of the seed layer under the interconnections on the crystallinity were investigated. As a result, the crystallinity was improved by changing the seed layer from Cu to Ru. In addition, the decrease in current density during electroplating also improved the crystallinity. Therefore, both introducing the Ru seed layer and decreasing the current density during electroplating are effective for developing highly reliable copper interconnections.

Smart Fiber Wireless (SFiWi) networks have attracted great attention due to their ability to become the next generation broadband access networks. FiWi networks integrate optical networks and wireless networks. Access networks consume much energy due to the increase of network participants and the volume of content. To conserve energy consumption in Passive Optical Network (PON), Optical Network Unit (ONU) sleep mode was proposed. The basic idea of ONU sleep mode is to enter sleep state, for an ONU sleep state period while the ONU is in idle situation. Traditional research on ONU sleep showed that long ONU sleep state period results only in increased latency. In this research we prove that excessively long ONU sleep state periods not only cause long latency but also result in more energy consumption. Furthermore, we derive the optimal sleep state period to minimize energy consumption. Also, we show that our derived sleep state period achieves better latency compared to large ONU sleep state period.

Four isolates tentatively identified as Pseudaegerita matsushimaeon the basis of the morphology of bulbil-like propagules were collected from substrates submerged in water in Thailand and Japan. In culture studies the two Thai isolates were found to produce phialoconidia on conidiogenous cells and phialoconidiophores whose morphology was similar to that of Trichoderma. Phylogenetic analysis based on D1/D2 regions of LSU rDNA sequences showed that the four isolates were nested in Hypocrea/Trichoderma(Hypocreales) while P. corticalis, the type species of Pseudaegerita, belongs to Hyaloscypha(Helotiales). Preliminary analysis by ISTH Web tools based on 5.8S-ITS rDNA and phylogenetic analysis based on rpb2and tef1-int4genes showed that the isolates have specific sequences of Trichoderma(anchors 1–5) and belong to the Hamatum clade but they grouped apart from any known species of Trichoderma. The sequences of the tef1-int4gene, which were amplified from the authentic specimen of P. matsushimae(IMI 266915), also showed that it belongs to the Hamatum clade closely clustering with T. yunnanensebut separate from our four isolates. The morphology of P. matsushimae(IMI 266915), especially the sizes of phialides and phialoconidia, were different from T. yunnanense. Thus, we conclude that IMI 266915 and our isolates are to be assigned to two different species in the Hamatum clade of Trichoderma, although both species have similar morphology of bulbils and phialoconidia. Morphology and molecular data revealed that P. matsushimaeshould be assigned to the genus Trichoderma as T. matsushimaeand the Thai and Japanese isolates are placed in T. aeroaquaticumsp. nov. This finding supports the interpretation that aero-aquatic fungi have evolved from terrestrial fungi. We assume that these fungi probably were derived from typically soil-inhabiting species of Trichoderma; an adaptation to aquatic environments is shown by formation of bulbil-like propagules floating on water.

Recent evidence suggests that cell therapy such as the injection of bone marrow–derived mononuclear cells (BMMNCs) can exert protective effects in various conditions associated with ischemia-reperfusion injury. Here, we investigate the effects of BMMNCs on the organ injurydysfunction induced by hemorrhagic shock (HS). Thirty-seven anesthetized male Wistar rats were subjected to hemorrhage by reducing mean arterial pressure to 35 ± 5 mmHg for 90 min, followed by resuscitation with 20 mLkg Ringer’s lactate administered over 10 min and 50 of the shed blood over 50 min. Rats were killed 4 h after the onset of resuscitation. Bone marrow–derived mononuclear cells were freshly isolated from rat tibias and femurs using Percoll density gradient centrifugation, and BMMNCs (1 × 107cells per rat in 1 mLkg phosphate-buffered saline, i.v.) were administered on resuscitation. Hemorrhagic shock resulted in significant organ injurydysfunction (renal, hepatic, neuromuscular) and inflammation (hepatic, lung). In rats subjected to HS, administration of BMMNCs significantly attenuated (i) organ injurydysfunction (renal, hepatic, neuromuscular) and inflammation (hepatic, lung), (ii) increased the phosphorylation of Akt and glycogen synthase kinase-3, (iii) attenuated the activation of nuclear factor-B, (iv) attenuated the increase in extracellular signal–regulated kinase 12 phosphorylation, and (v) attenuated the increase in expression of intercellular adhesion molecule-1. Our findings suggest that administration of BMMNCs protects against the induction of early organ injurydysfunction caused by severe HS by a mechanism that may involve activation of Akt and the inhibition of glycogen synthase kinase-3 and nuclear factor-B.

We fabricated wavelength tunable laser diodes consisting of ring resonator wavelength filters with Si-wire waveguides. More than 45 nm wavelength tuning operation was obtained by about 100 mW heating power. We also measured spectral linewidth of the tunable laser, and observed about 200 kHz of spectral linewidth.

Two isolates, AC04Tand AC05, were isolated from the flowers of red ginger collected in Chiang Mai, Thailand. In phylogenetic trees based on 16S rRNA gene sequences, the two isolates were included within a lineage comprised of the genera Acidomonas, Gluconacetobacter, Asaia, Kozakia, Swaminathania, Neoasaia, Granulibacter, and Tanticharoenia, and they formed an independent cluster along with the type strain of Tanticharoenia sakaeratensis. The calculated pair-wise sequence similarities of isolate AC04Twere 97.8–92.5% to the type strains of the type species of the 11 genera of acetic acid bacteria. The DNA base composition was 66.0–66.1 mol % G+C with a range of 0.1 mol %. A single-stranded, labeled DNA from isolate AC04Tpresented levels of DNA-DNA hybridization of 100, 85, 4, and 3% respectively to DNAs from isolates AC04Tand AC05 and the type strains of Tanticharoenia sakaeratensisand Gluconacetobacter liquefaciens. The two isolates were unique morphologically in polar flagellation and physiologically in intense acetate oxidation to carbon dioxide and water and weak lactate oxidation. The intensity in acetate oxidation almost equaled that of the type strain of Acetobacter aceti. The two isolates had Q-10. Isolate AC04Twas discriminated from the type strains of the type species of the 11 genera by 16S rRNA gene restriction analysis using restriction endonucleases TaqI and Hin6I. The unique phylogenetic, genetic, morphological, physiological, and biochemical characteristics obtained indicate that the two isolates can be classified into a separate genus, and Ameyamaea chiangmaiensisgen. nov., sp. nov. is proposed. The type strain is isolate AC04T(=BCC 15744T, =NBRC 103196T), which has a DNA G+C content of 66.0 mol %.

The mechanism of the directional coarsening of ' phases (rafting) of Ni-base superalloy under an uni-axial strain was analyzed by molecular dynamics (MD) analysis. The stress-induced anisotropic diffusion of Al atoms perpendicular to the interface was observed clearly in a Ni(001)/Ni3Al(001) interface structure, The reduction of the diffusion of Al atoms perpendicular to the interface is thus, effective for improving the creep and fatigue resistance of the alloy. It was also found that the dopant elements in the superalloy also affected the strain-induced diffusion of Al atoms. Pd was one of the most effective elements which restrain Al atoms from moving around the interface.

Electrical conductivity of 10 mol% yttria-stabilized zirconia (10YSZ) and YBa2Cu3O7-d (YBCO) under stress were measured in the temperature range from 600oC to 800oC for 10YSZ and at 600oC for YBCO. It was observed that conductivity under stress slightly decreased for 10YSZ and increased for YBCO. In the case of 10YSZ, the conductivity change was caused by the change in mobility. In the case of YBCO, the conductivity change was caused by the change in mobility and change in carrier concentration.

A new hybrid phosphine was designed. The phosphine combines two common structural characteristics found among the effective phosphine ligands reported recently, namely, three tert-alkyl substituents binding to the phosphorus and an aryl group at an appropriate position. A hybrid phospine/palladium system is versatile and effective for the coupling reaction of various aryl halides with primary and secondary amines including carbazole.

Tanticharoenia sakaeratensisgen. nov., sp. nov. is proposed for three strains isolated from soil collected in Thailand. The three strains, AC37T, AC38, and AC39, were included within a lineage comprising the genera Asaia, Kozakia, Swaminathania, Neoasaia, Acetobacter, Gluconobacter, and Saccharibacterin a phylogenetic tree based on 16S rRNA gene sequences, but formed a quite different, independent cluster. Pair-wise sequence similarities of strain AC37Twere 96.5–92.1% to the type strains of Acetobacter aceti, Gluconobacter oxydans, Acidomonas methanolica, Gluconacetobacter liquefaciens, Asaia bogorensis, Kozakia baliensis, Swaminathania salitolerans, Saccharibacter floricola, Neoasaia chiangmaiensis, and Granulibacter bethesdensis. The three strains had DNA base compositions comprising respectively 65.6, 64.5, and 65.6 mol % G+C with a range of 1.1 mol %, and formed a single species. Phenotypically, the three strains did not oxidize acetate or lactate, but grew on 30% D-glucose (w/v). Chemotaxonomically, they had Q-10. The type strain is AC37T(= BCC 15772T= NBRC 103193T).

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