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Subcutaneous Sweet’s syndrome (SS) is a rare variant of classic SS characterized by a neutrophilic infiltrate exclusively or predominantly in the subcutaneous tissue, with minimal or absent dermal involvement. We report the case of a patient with a history of chronic myelogenous leukemia who developed subcutaneous SS. Although it has been described in patients with myelodysplastic syndromes and acute myeloid leukemia, this is the first report, to our knowledge, of this rare entity occurring in the setting of a myeloproliferative disorder.

In the past decade, 49 states have adopted rigorous curriculum standards. This has resulted in a significant impact on special education policy and practice. A research team consisting of 12 doctoral students and a faculty advisor at the College of William and Mary in Williamsburg, Virginia, reviewed published state standards, primary assessment procedures, and provisions related to participation by students with disabilities, to determine answers to six questions: (a) Have states adopted standards assessment criteria? (b) Are states using performance rewards and sanctions to motivate educators and students? (c) Do states require all students with disabilities to participate in the state assessment process? (d) Have states developed policies regarding testing accommodations for students with disabilities? (e) Do states link assessment performance to high school graduation? (f) How are states approaching alternate assessments? Research across states found differences in the ways in which assessment issues are being addressed and the potential impact that these policies will have on educational opportunities for students with disabilities.

Additional copies of virulence (vir) genes do enhance transformation frequencies in multiple species; the mechanisms that play a role in this enhancement are not understood. In this report, transformants produced using additional copies of helper plasmids harboring pCH30 (virG), pCH32 (virG and virE), and pCH42 (virE) were evaluated for gene expression, copy number, and complexity of integration patterns. The virplasmids increased transformation efficiency 2-fold with pCH42, 3-fold with pCH30, and 4-fold with pCH32. One to six transgene copy numbers were detected, and irregular transgene integration patterns were observed in all plasmid combinations (pAGM280 only, pAGM280 + pCH30, pAGM280 + pCH32 and pAGM280 + pCH42). There were no differences in transgene copy number and/or integration patterns between transformants produced with or without additional virgenes. Additionally, no relationship between transgene copy number and expression or between transgene integration patterns and expression was observed. All transgenic plants resulting from the additional virplasmids were able to express the foreign genes. In contrast, forty percent of transgenic plants regenerated without additional virplasmids did not express the foreign gene even though the foreign genes were present. These results demonstrate a link between an increase in copy number of the virgenes and stable transgene expression and increased plant transformation efficiency.

Studies were conducted to investigate the nitrification potential of an industrial wastewater that contained high amounts of ammonium sulfate. Laboratory-scale, activated-sludge reactors were unable to nitrify the wastewater, but the same sludge began to nitrify within 2 weeks after the feed was changed to a synthetic wastewater. Further investigation indicated that oxime compounds in the industry's wastewater were causing nitrification inhibition. Four oximes known to exist in the industrial wastewater were studied: cyclohexanone oxime was not inhibitory up to 100 mg/L, acetaldehyde oxime and aldecarb oxime were moderately inhibitory, and methylethyl ketoxime (MEKO) was strongly inhibitory to nitrification. The mechanism of MEKO inhibition was evaluated using three different analytical approaches. Methylethyl ketoxime demonstrated pure noncompetitive inhibition patterns using all methods of data analysis. The inhibition constant Kiindicates the concentration that causes 50% inhibition and was estimated to range from 0.3 to 1.9 mg/L at 25 °C for MEKO.

This review examines the literature on successful establishment of regenerable cell cultures in the economically important cereal crops. Some of the major trends and strategies for the establishment of in vitro cultures that express totipotency are discussed as well as current approaches. It is apparent that in cereal tissue culture, not all cells express totipotency. Generally the auxin 2,4‐dichlorophenoxyacetic acid (2,4‐D) is critical for the production of regenerable callus; however, the addition of cytokinin can be significant. Meristematic cells from immature tissues are the targets for plant growth regulator action. Some genotypes produce embryogenic cultures, while others are recalcitrant to in vitro manipulation. Regeneration occurs either by somatic embryogenesis or adventitious bud and shoot development with subsequent rooting. In these meristematic tissues, plastids are at the undifferentiated proplastid stage of development. The development of a white, nodulated embryogenic callus in somatic embryogenesis and the formation of green buds during organogenesis suggest divergent modes of plastid differentiation during morphogenesis. Plant growth regulators may be involved with inducing or directing different pathways of plastid differentiation. Genotypic differences in morphogenesis may be due to differences in endogenous hormone levels.

A cross-sectional comparison was made of the responses of 1,110 terminally ill persons with cancer at varying time periods prior to death. In addition, a longitudinal analysis on a subgroup of this sample was conducted for self-reported mood changes and social interaction changes as death approached. Two theories, Disengagement and Objective Self-Awareness, which suggest that people nearing death would increase in their desire to separate from others, were not supported. In general, there were few differences in the responses of persons further from, or closer to, death. The mention of religion as a source of support was less common among persons closer to death. Finally, awareness of one's demise had an effect only on one's responses regarding the role of other people in the life of the terminally ill person.

This paper establishes that the isolated shoot meristem of monocotyledons can be infected and transformed using Agrobacterium. Since this explant from nearly any cereal cultivar can rapidly regenerate into a plant, using this explant effectively eliminates the genotype regeneration restrictions to cereal crop transformation allowing direct transformation of elite germplasm. Shoot apices of Oryza sativa L. Tropical Japonica, cv. Maybelle were explants used for cocultivation, and gene transfer was accomplished using Agrobacterium containing plasmids for the bar gene expression driven by the CaMV 35S promoter or by the rice actin 1 promoter. Experiments to determine the survival rates of isolated shoot apices on media containing the herbicide, glufosinate-ammonium (PPT), established that no shoot apices survived on 0.5 or 1.0 mg/l PPT. After shoot apices were cocultivated with Agrobacterium, 2.8% (overall 20 out of 721 shoot apices) survived on 0.5 mg/l PPT. Results demonstrated that the use of the actin 1 promoter-based expression vector and an extra-wounding treatment of the meristematic cells appeared to be most effective in promoting transformation. Integration, expression and transmission of the transferred foreign genes in primary, R1 and R2 generation plants were confirmed by molecular analyses and herbicide application tests. A germination test of R2 progeny from one of the transgenic plants (R1) established a phenotype segregation ratio showing a non-Mendelian inheritance pattern. Inactivation of the transferred foreign gene in R2 progeny appeared to result from transgene methylation.

Agrobacterium tumefaciens(Smith and Townsend, 1907) has been an extremely useful vector to transfer foreign genes into dicotyledonous plants. Monocotyledonous plants, particularly the cereals, have been considered outside the host range for A. tumefaciens, which has necessitated the development of other transformation systems such as naked DNA delivery to protoplasts and, most recently, microprojectile bombardment delivery of DNA to cells and tissues. Both systems have worked, but there are still many difficulties encountered in routine transformation of any monocotyledon. Recently, there has been renewed interest in using the A. tumefacienssystem to transform economically important grasses and other monocotyledons. This paper examines the literature and steps involved in transformation of monocotyledons by A. tumefaciens. The many recent advances in understanding the biology of the infection process (meristematic target cell, virgene inducing compounds, and wide host range strains of A. tumefaciens), and availability of more monocotyledon gene promoters and improved selectable markers greatly improve the opportunities of developing monocotyledon transformation systems with A. tumefaciens.

No abstract available for this article.

In this secondary analysis of data from the National Hospice Study (NHS), a new measure, quality of death (QOD), was developed by weighting reports of cancer patients' last 3 days of life by what patients wanted their last 3 days to be like. Using analysis of covariance, the QOD scores were higher for terminally ill patients in hospices (either home-care HC or hospital-based HB) than similar patients who received conventional care (CC). The results are discussed in terms of verification of the hospice philosophy and other uses for a quality of death measure.

Proteins obtained from seedling shoots and floral meristems ofSorghum bicolor (L.) Moench cv. NK 1210 induced mycelial growth in the smut fungus,Sporisorium reilianum in vitro. Proteins precipitated with trichloroacetic acid and ammonium sulfate were equally effective as inducers, although there were minor variations in the pattern of mycelial growth. Hydrolysis of the protein fraction with the proteolytic enzyme pronase E resulted in considerable reduction in the proteins' ability to induce mycelial growth. Digestion of the protein fraction with driselase, resulted in a slight enhancement of biological activity. The results suggest that amino sugar moieties in glycoproteins may act as inducers of mycelial growth inSporisorium reilianum.

Abstract: β-glucuronidase (GUS) can be assayed in the spent media of plant tissues transformed with some GUS gene fusions (Jefferson, 1988). This approach is based on the presence of GUS in the media of transformed plant tissues expressing the gene and can be used to monitor the progress of transformation without destruction of the tissue under study.

The desire for control over one’s health care is defined as preferring to directly influence the nature of that care. We attempted to measure the following relevant constructs and to examine the relationships among them: (1) desire-for-control (DCON) in a specific health care setting—a hypothetical situation in which a person was facing imminent death; (2) attitude toward an action perceived as enhancing control in that situation—signing a living will; and (3) intention to exercise control by performing that action—actually signing a living will. An analysis of variance (DCON x attitude) of intention to sign a living will yielded statistically significant main effects for DCON and attitude, although DCON was in a direction counter to that predicted. Possible explanations for the DCON finding were explored.

Coleus blumeiBenth. primordial leaves 1 through 4 and expanding leaves 5 to 8 were isolated and cultured to examine the effects of auxin and kinetin on development. Without the plant growth regulators in the medium, expanding leaves 7 and 8 developed as leaves; younger leaf primordia did not develop. With 0.01 to 5.0 mg/1 IAA, 2–7% of the youngest pair of primordial leaves (1 and 2) developed as roots. Small leaf blade development occurred on IAA at 0.5 to 5.0 mg/1 with 10–12% of the explants, and shoots developed from 2% of the youngest primordia explants at 3 mg/1 IAA. With 2–28% of the second set of primordial leaves (3 and 4), a leaf with a root developed with 0.01 to 5.0 mg/1 IAA. At 3.0 mg/1 IAA, in addition to leaf formation, 2% of the explants formed a rosette of leaves and 1% formed a shoot. With the highest level of IAA (5 mg/1), 2% of the explants formed a root. Expanding leaves 5 through 8 developed mostly into leaves without petioles on IAA and kinetin. Plant development occurred from 2% of the youngest primordial leaves on 0.03 mg/1 kinetin; otherwise, these primordia on 0.003 to 2 mg/1 kinetin developed into abnormal leaves. Primordia 3 and 4 developed into normal appearing leaves at levels of kinetin between 0.03 and 2 mg/1. At lower levels the leaves were abnormal.

Summary: Selectable marker genes play an important role in plant transformation. The level of selection pressure is generally established by generating a kill curve for the selectable marker. In most cases, the lowest concentration which kills all explants is used. This study examined two selectable marker genes, phosphinothricin acetyl transferase (PAT) and hygromycin phosphotransferase (HPT), in transformation of tobacco leaf disks. Experiments to determine the lethal level of the herbicide, glufosinate-ammonium (phosphinothricin) (PPT) using a leaf-disk regeneration assay established that no shoots regenerated at 2 to 4 mg PPT per 1. Likewise with the antibiotic, hygromycin (HYG), no plants regenerated at 50 mg hygromycin per 1. In contrast, after cocultivation of the leaf disks withAgrobacterium tumefaciens containing either the PAT or HPT gene in combination with a Bt gene for insect resistance, plants were successfully regenerated from leaf disks at 2 to 4 mg PPT per 1 and 50 mg hygromycin per 1. However, most plants regenerated at 2 and 3 mg PPT per 1 were found to be nontransformed (95–100% escapes) by i) Southern-blot analysis, ii) herbicide application test, and iii) insect feeding bioassay. On the other hand, plants that regenerated on 50 mg hygromycin per 1 and 4 mg PPT per 1 were transgenic as determined by Southern analysis, leaf assay for PPT or HYG resistance, and death of tobacco budworms feeding on these leaves. This study showed a significant level of cross-protection and/or transient expression of the PAT selectable marker gene allowing escapes (95–100%) at selection levels of 2 and 3 mg PPT per 1 which completely kill controls. On the other hand, the HPT gene at 50 mg is efficient in selecting for T-DNA integration.

Glycoprotein changes following developmental transition from vegetative to floral initiation were detected in the meristematic tissue of sorghum head smut reaction classes (R1, R2, R3 and S1) by six biotinylated lectins (BS-I, Con A, PNA, SBA, UEA-I, and WGA) after sodium dodecyl sulfate-gel electrophoresis and protein blotting. Screening using biotinylated lectins indicated that BS-I, PNA, and SBA showed binding to glycoproteins (which may be due to α-d-Gal, β-d-Gal[1–3]-d-gal Ac, and/or D-GalNAc) only from the vegetative meristem. Conversely, Con A showed binding to glycoproteins (which may be due to α-d-Glc and α-d-Man) only from the reproductive meristem. These BS-I, PNA, SBA, and Con A-binding reactions did not show any differences among R1, R2, R3, and S1. UEA-I (known to react with α-l-Fuc) binding was detected in all sorghum head smut reaction classes in vegetative meristems, but only in R2 and R3 in reproductive meristems. WGA (known to react with β-d-GlcNAc) binding was not detected in either vegetative or reproductive meristems.

Summary: Histologic examination of shoot-tip explants, 1 wk after culture initiation on Murashige and Skoog medium with 2.5 mg/liter 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.05 mg/liter kinetin, reveals active meristematic centers inside cultured tissue. Clusters of cells in these meristematic centers exhibit remarkable resemblance to the initial three divisions in the zygotic embryo. Several such meristematic groups of cells are observed in the cultured explant at this stage. Embryogenesis is obviously initiated very early in this tissue in the presence of 2,4-D. A well-defined, white globular embryogenic callus develops in culture in about 4 wk, and it consists of clusters of embryoids with large cells characterized by thick cell walls, numerous lipoidal vesicles, and localized areas of carbohydrate storage. These cells resemble the scutellar tissue of the embryo. However, there are cells within this tissue that themselves appear embryogenic. They undergo cell division giving rise to small clusters of cells. As long as 2,4-D is present in the medium, the cells apparently retain the capacity to proliferate and to produce more cells capable of embryogenesis. Embryogenesis seems to occur via two processes, initiation of somatic embryos early in culture and secondary embryogensis from the scutellar tissue that forms in vitro.

Summary: Maintainable, highly embryogenic suspension cultures of a wild relative of cotton (Gossypium klotzschianum Anderss.) have been obtained. Callus with no apparent organization was used to establish the liquid culture. Callus growth conditions as well as suspension medium composition were optimized. A visual selection scheme was beneficial for the maintenance of the embryogenic suspension. These liquid cultures have been maintained for over 10 mo. with no loss in embryogenic capacity. The somatic embryos developed after transfer of the embryogenic tissues to a hormone-free liquid medium.

Data from four studies of adults are used to address the relationship between expectancies for control of one's health (an outcome) and preference for control of health care (a process). The former is operationalized by the use of the Multidimensional Health Locus of Control (MHLC) Scales; the latter by the B and I subscales of the Krantz Health Opinion Survey (KHOS). Descriptive data from the four studies are presented. Significant correlations exist between the B subscale and the PHLC and IHLC scales. Factor analysis of datafrom three of the studies reveals that, along with selected items from other scales, all of the items from both the PHLC scale and the B subscale load on the first factor. Both theoretical and methodological reasons for these findings are discussed.

Sporisorium reilianum, the fungus that causes sorghum head smut, was grown with sucrose, lactose, trehalose or raffinose in liquid suspension or on a solid medium. Liquid culture media were analyzed for hydrolysis products of these carbohydrates to determine extracellular enzyme activity of the fungus. Increased amounts of glucose and fructose in the culture medium ofS. reilianum grown with sucrose or raffinose indicated that invertase (ß-fructofuranosidase, 3.2.1.26) activity was present. No evidence of extracellular galactosidase or trehalase activity was found. Enhanced sporidial colony formation on carbohydrates that can be hydrolyzed to hexoses, and specific forms of mycelial growth on lactose, trehalose or on a carbohydrate-deficient medium might suggest that mycelial growth is a way of foraging for food sources. However, the rapid and profuse mycelial growth on the host cell wall glycoprotein appears to be in response to abundant food supply (probably of a different type). Therefore availability of different kinds of carbon sources in the environment of the growing fungus might determine dimorphism and associated pathogenesis byS. reilianum.

Coleus blumeiBenth. apical meristems and apical meristems +1, +2, +3 primordial leaf pairs were cultured to examine phytohormone influences on development and correlative effects of developing primordial leaves on in vitro responses. The meristem with no phytohormones or low levels of IAA could not develop in vitro. At least 0.1 mg/l IAA and optimumly 1‐2 mg/l IAA were required for development into complete plants. IAA from 0.1 to 3 mg/l also resulted in root development with no apparent leaf or shoot formation. Levels of IAA higher than 3 mg/l were inhibitory to development. Kinetin, as a substitute for naturally occurring cytokinins, alone (0.0003 to 3 mg/l) resulted in development of rosettes of leaves. In the presence of IAA (***1 mg/l) and kinetin (0.003 mg/l) plants, rosettes, individual leaves with roots, and roots developed from isolated meristems. Glutamine and adenine sulfate both appeared inhibitory to meristem development. With +1, +2, +3 developing primordial leaf pairs left attached to the apical dome, three pairs were required for plant formation in the absence of phytohormones. In the presence of IAA, two pairs of primordial leaves resulted in plant formation; whereas, with IAA and low levels of kinetin one pair of primordial leaves was enough. Higher levels of kinetin were inhibitory to plant development with primordial leaves present. ABA appeared to be inhibitory to development of meristems and meristems +1, +3 primordial leaves at low concentrations and resulted in death at ***1 mg/l. Developing primordial leaves appear to supply the apical meristem with a balance of phytohormones during growth. Meristem development into a plant first involved formation of leaf primordia. Establishment of a bipolar axis with root formation followed.

Cotton (Gossypium hirsutum L.) and kenaf ( Hibiscus cannabinus L.) belong to the Malvaceae family, and both are used as sources of fibers. Shoot apices from vigorous seedlings aseptically germinated from 3 different cultivars of both cotton and kenaf were used in this study. The cotton and kenaf shoot apex size was between 2–3 mm containing the meristem, unexpanded leaves, and a small portion of the cotyledon. Shoot apices were placed on 18 different media comparing full and 1/2 strength Murashige and Skoog (1962) plus vitamins, and combinations of 0, 0.1, and 1 mg l-1of naphthaleneacetic acid and 6-benzyladenine (BA). The shoot apices of both crops developed successfully without intervening callus formation, and no significant differences among cultivars were found. An average of 58% of the cotton shoot apices initiated shoot and rooted in full strength Murashige and Skoog (1962) plus vitamins in 6 weeks. For kenaf, an average of 92% of shoot apices initiated shoot and rooted in full strength Murashige and Skoog plus vitamins and 0.1 mg l-1BA in 3 weeks. All regenerated plants of both crops were phenotypically normal and set seeds.

Summary: Arachis hypogaea L. peanut, has been a difficult species to manipulate in tissue culture. Lack of a reliable and quick regeneration method for peanuts has proven to be one of the hindrances in the application of transformation protocols to the crop. A protocol to initiate shoot apex elongation and rooting of these shoots is described. This protocol was successful with two peanut cultivars. Shoot apices were isolated from germinated seedlings and placed on Murashige and Skoog salts containing N6-benzyladenine for shoot initiation. Once shoot elongation occurred, the explant was transferred to a rooting medium containing Murashige and Skoog salts and only one plant growth regulator, α-naphthalene acetic acid. In as few as 3 weeks, the explants began to root and could be transferred to soil. Forty-five percent of explants isolated from germinating peanut seeds would root on this medium. Elongation and rooting of the shoot apices were not hindered by the addition of an antibiotic to the medium, indicating that the regeneration method could be useful inAgrobacterium tume-faciens-mediated transformation protocols.

Summary: A tissue culture method was developed for the eradication of three important potato viruses, PVX, PVY, and PVS, from the Russet Norkotah variety and two of its strains (TXNS 112 and TXNS 278). The method combined the use of liquid medium, thermotherapy, and chemotherapy. Initially, virus-free plants were inoculated with PVX, PVY, and PVS and, after 10 d, tested quantitatively for virus titer by ELISA to determine the initial virus concentration. The apical tip and the roots were removed from the inoculated plants, and only stem sections from inoculated plants were cultured in liquid medium containing MS inorganic salts, vitamins, and ribavirin (40 M or 80 M). After 5 d, half of the plants were subjected to thermotherapy and half were kept at room temperature. At 6 wk, the uppermost node (5–7 mm) was removed and recultured, and plants were tested then and again after 6 wk using ELISA to identify the virus-free plants. Ribavirin alone eradicated the viruses from some plants; however, more virus-free plants were obtained when the treatments included heat. Additionally, thein vitro culture technique using liquid medium promoted rapid lateral shoot elongation and resulted in significantly faster plant production. Also this approach, which required less skilled labor, produced more plants than the meristem culture method for virus eradication. A detailed procedure for elimination of multiple viruses is described. This procedure resulted in production of more than 10% virus-free plants.

Summary: A method of clonal propagation via somatic embryogenesis of date palm, cultivar Barhee, which has potential for large scale commercial application as well as for developmental studies on embryos is described. Cultures were initiated from shoot tip and immature inflorescence explants, both of which were capable of development into embryogenic callus. When the embryogenic callus was cultured in liquid suspension on a rotary shaker, hundreds of embryos developed from milligram quantities of callus in a fairly synchronous manner. Scanning electron microscopy showed globular, heart-shaped and torpedo-shaped embryos. Green leaves emerged from a white cotyledonary sheath.

Summary: Shoot tip cultures from 2- to 3-d-old seedlings ofSorghum bicolor (L.) Moench cv. IS3620C develop highly embryogenic callus from which plants can be regenerated when transferred to plant growth regulator-free medium. Isolated shoot tips were cultured on Murashige and Skoog medium supplemented with 2.5 mg/liter 2,4-dichloro-phenoxyacetic acid and 0.05 mg/liter kinetin. Purple pigmentation characteristic of sorghum cultures on growth regulator-free medium is virtually eliminated with the shoot tip culture. Embryogenic callus is white and hard with an undulating appearance but can be separated into multiple bipolar structures by application of gentle pressure. The well-developed embryos have a cup-shaped scutellum. These germinate like zygotic embryos and develop root-shoot axis. Lack of vascular connections to the parent tissue and the synchronous development of the plumule and radicle suggest that these embryos may be of unicellular origin. In contrast, when the entire seedling serves as the explant, all meristematic centers in the shoot, including the coleoptile sheath close to the apical meristem respond to plant growth regulators in the medium by callus formation. Upon subsequent reculture onto growth regulator-free medium several modes of development occur. The differential response of these tissues to identical culture conditions indicate the presence of different population of cells that respond differently to exogenous plant growth regulators.

Embryogenesis and plant regeneration of Texas and international rice, Oryza saliva (L.), varieties (both indica and japonica types) were induced in culture on a regime consisting of the use of ABA or BAP in the subculture medium and small (10 mg) callus pieces on the regeneration medium. Ten 10 mg callus pieces on regeneration medium resulted in a 2- to 10-fold increase in plant regeneration over single 100 mg pieces. Plant regeneration of Texas rice cultivars (Lemont, Rico I, Rexmont and Skybonnet) and Taipei 309 was enhanced by the use of ABA in the subculture medium with a 2-fold and a 3- to 10-fold increase in plant regeneration with 2.6 mgL-1 and 26 mgL-1 ABA in the subculture media, respectively. Regeneration of plants from callus of IR36 and IR64 was not enhanced by ABA but by the use of BAP and Trp in the subculture medium or by 2,4-D alone. The subculture medium containing BAP and Trp produced a 5-fold increase in plant regeneration rate from IR64 callus and was equal to subculture medium containing only 2,4-D for IR36 callus. Both Lemont and IR36 were previously reported to be difficult to regenerate or non-regenerative, however, the use of ABA or BAP in the subculture medium, small callus pieces and visual selection of embryogenic callus allowed the regeneration of up to 20 and 22 plants from 100 mg of Lemont and IR36 callus, respectively.

A method of regenerating cotton plants from the shoot apical meristem of seedlings was developed for use with particle gun and Agrobacterium-mediated transformation. This method was developed to circumvent the problems of genotype restriction and chromosomal damage frequently encountered in cotton regeneration in tissue culture through somatic embryogenesis. In this procedure, the cells of the shoot meristem are targeted for transformation. Normal and fertile plants of Gossypium barbadense Pima S-6, and 19 cultivars of G. hirsutum were regenerated using this method. Shoot regeneration from these tissues was direct and relatively rapid. A MS based, hormone-free medium could be used with all the varieties tested.

No abstract available for this article.

Development of complete plants was achieved from isolated shoot apical meristems of Nicotiana tabacumL., Daucus carotaL., Nicotiana glaucaGrah., Tropaeolum majusL., and Coleus blumeiBenth. The explants consisted of only meristematic dome tissue with no visible leaf primordia. A simple nutrient medium composed of the Murashige and Skoog salt mixture, 100 mg/liter myo‐inositol, 0.4 mg/liter thiamin‐HCl, 1‐2 mg/liter IAA, 30 g/liter sucrose, and 1% agar was adequate. Histologically there occurred principally tissue enlargement during the first 3‐6 days, followed by appearance of bipolar organization in 6‐9 days and formation of a well‐defined root apex and initiation of first leaf primordium by 12 days.

The article discusses on the artistic profile and the background of the paintings of Brice Marden. It states that Marden usually starts a painting with a thought of a certain quality that he has found elsewhere, whether in other artwork, in a landscape, in a photograph, or in a relationship. It is considered that his work since 1965 consists of one, two, and finally three-panel paintings.

Somatic embryoids differentiated in suspension cultures of G. klotzschianum after 3–4 weeks of culture in a liquid medium containing glutamine (optimally, 10–15 mM). Embryogenesis occurred after a preculture of callus on a medium containing 10 mg/l of the cytokinin, 2iP. The embryoids had meristematic regions, a well formed epidermis, and formed roots and vestigial leaves. Asparagine was much less effective than glutamine in promoting embryoid differentiation. The presence of 2,4-D in the medium resulted in increased vigor of the suspension cultures and subsequently in the formation of many embryoids, but does not seem to be necessary for somatic embryogenesis in cotton.

Stem and petiole sections from a diploid species of cotton (Gossypium klotzschianum Anderss.) were longitudinally sliced and placed on a callus induction medium. Explants with the cut surface in contact with the medium either browned or produced a slow growing red callus which could not be subcultured. Explants oriented with the epidermis in contact with the medium produced a rapidly growing, friable, green callus which could be subcultured biweekly and has been maintained for 12 months. Globular and torpedo embryos were obtained 2 weeks after transfer of the callus to a liquid medium containing the auxin, 2,4-dichlorophenoxyacetic acid. Following subculture to an auxin-free medium, the embryos underwent additional development but plantlets were not obtained. Attempts to consistently regenerate plants from embryos are currently under way.

The article presents information on drawings displayed at art galleries and museums in the U.S. Presented are drawings including the 1908 "Underpass," by Joseph Stella, the 1913 "Military Symbol," by Marsden Hartley and the 1926 "The Artist's Mother," by Arshile Gorky. It is noted that the art received significant attention from New York Museums in 1973 where drawings by 87 American artists were exhibited. The author notes that since 1946, there has been varying integration between painting, drawing and sculpture.

The article reviews several art exhibitions in New York City, including exhibits by Robert Rauschenberg at the Castelli Gallery and Sonnabend Gallery, by Joe Zucker at the Bykert Gallery and by Bruce Boice at the Sonnabend Gallery.

The article reviews several art exhibitions featuring the works of Christian Boltanski, Mario Merz and Jules Olitski.

The article discusses public art, examining public sculpture that has been created between 1993-2008. Public sculpture has become contemporary art's more exciting areas, the author states. Topics include the work "Cloud Gate," by artist Anish Kapoor, which is located in Chicago, Illinois at Millennium Park.