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A non-destructive assay for GUS in the media of plant tissue cultures

Authors :
Gould, Jean
Smith, Roberta
Source :
Plant Molecular Biology Reporter; August 1989, Vol. 7 Issue: 3 p209-216, 8p
Publication Year :
1989

Abstract

Abstract: β-glucuronidase (GUS) can be assayed in the spent media of plant tissues transformed with some GUS gene fusions (Jefferson, 1988). This approach is based on the presence of GUS in the media of transformed plant tissues expressing the gene and can be used to monitor the progress of transformation without destruction of the tissue under study.

Details

Language :
English
ISSN :
07359640 and 15729818
Volume :
7
Issue :
3
Database :
Supplemental Index
Journal :
Plant Molecular Biology Reporter
Publication Type :
Periodical
Accession number :
ejs14996171
Full Text :
https://doi.org/10.1007/BF02668687