Search

Skeletal muscle regeneration is a complex process where various cell types and cytokines are involved. Single-cell RNA-sequencing (scRNA-seq) provides the opportunity to deconvolute heterogeneous tissue into individual cells based on their transcriptomic profiles. Recent scRNA-seq studies on mouse muscle regeneration have provided insights to understand the transcriptional dynamics that underpin muscle regeneration. However, a database to investigate gene expression profiling during skeletal muscle regeneration at the single-cell level is lacking. Here, we collected over 105 000 cells at 7 key regenerative time-points and non-injured muscles and developed a database, the Single-cell Skeletal Muscle Regeneration Database (SCSMRD). SCSMRD allows users to search the dynamic expression profiles of genes of interest across different cell types during the skeletal muscle regeneration process. It also provides a network to show the activity of regulons in different cell types at different time points. Pesudotime analysis showed the state changes trajectory of muscle stem cells (MuSCs) during skeletal muscle regeneration. This database is freely available at https://scsmrd.fengs-lab.com.

The spontaneous decay dynamics of an excited emitter embedded in woodpile photonics crystals (PCs) are investigated by a plane-wave expansion method based on non-Markovian approximation. We find that the spontaneous decay properties are different when emitters are in different positions in high-refractive-index-ratio woodpile PCs and the emitter can be partially trapped in the steady state. Our analysis indicates that the formation of a bound state between the emitter and its structure environment results in this fractional population. Our results may be supplied as reference results for probing the local density or direction local density of states in future experiments.

We present an efficient method for calculating the orientation-dependent local density of states (ODLDOS) or local density of states (LDOS) in two-dimensional photonic crystals by combining the lattice point group transformation of the vector field and the special point integral with an interpolation method. This method can swiftly compute LDOS or ODLDOS and acquire the same results as those computed based on a larger number of $\mathbf{k}$ points in the first Brillouin zone. Our method improves the calculation speed by about 1000 times.