61 results on '"Lamerz, A."'
Search Results
2. Pharmacological Enhancement of Adult Hippocampal Neurogenesis Improves Behavioral Pattern Separation in Young and Aged Male Mice
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Chang, Wei-li, Tegang, Karly, Samuels, Benjamin A., Saxe, Michael, Wichmann, Juergen, David, Denis J., David, Indira Mendez, Augustin, Angélique, Fischer, Holger, Golling, Sabrina, Lamerz, Jens, Roth, Doris, Graf, Martin, Zoffmann, Sannah, Santarelli, Luca, Jagasia, Ravi, and Hen, René more...
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Impairments in behavioral pattern separation (BPS)—the ability to distinguish between similar contexts or experiences—contribute to memory interference and overgeneralization seen in many neuropsychiatric conditions, including depression, anxiety, posttraumatic stress disorder, dementia, and age-related cognitive decline. Although BPS relies on the dentate gyrus and is sensitive to changes in adult hippocampal neurogenesis, its significance as a pharmacological target has not been tested. more...
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- 2025
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3. QbD Approach to Process Characterization and Quantitative Criticality Assessment of Process Parameters.
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Rege, Pankaj D., Schuster, Andreas, Lamerz, Jens, Moessner, Christian, Göhring, Wolfgang, Hidber, Pirmin, Stahr, Helmut, Andrei, Oana Mihaela, Burren, Janine, Moesching, Alexandre, Coleman, Daniel, and Hildbrand, Stefan more...
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- 2024
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4. Development of a Continuous Flow Grignard Reaction to Manufacture a Key Intermediate of Ipatasertib
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Kaldre, Dainis, Stocker, Severin, Linder, David, Reymond, Helena, Schuster, Andreas, Lamerz, Jens, Hildbrand, Stefan, Püntener, Kurt, Berry, Malcolm, and Sedelmeier, Jörg
- Abstract
This article outlines the development of a continuous flow process for the manufacture of a key intermediate of the active pharmaceutical ingredient ipatasertib for the treatment of metastatic castration-resistant prostate cancer and triple-negative metastatic breast cancer. The reaction sequence incorporates multiple telescoped unit continuous operations, including a Br/Mg exchange reaction leading to intramolecular cyclization of the magnesium species onto a neighboring nitrile group to form a five-membered ring in 5-exo-dig fashion. The product from the reaction mixture is obtained after continuous aqueous acidic hydrolysis, neutralization/extraction, water wash, and phase separation. Each of these unit operations took place in a cascade of continuous stirred tank reactors. The control strategy was refined via a series of continuous lab studies at 20 g/h using a Design of Experiments approach to define process parameter ranges and to help identify any criticality therein. The learnings from this laboratory study served as a basis for the construction of a suitable pilot-plant facility, where the control strategy was verified at a representative manufacturing scale of about 1.0 kg/h. more...
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- 2024
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5. QbD Approach to Process Characterization and Quantitative Criticality Assessment of Process Parameters†
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Rege, Pankaj D., Schuster, Andreas, Lamerz, Jens, Moessner, Christian, Göhring, Wolfgang, Hidber, Pirmin, Stahr, Helmut, Andrei, Oana Mihaela, Burren, Janine, Moesching, Alexandre, Coleman, Daniel, and Hildbrand, Stefan more...
- Abstract
The quality-by-design (QbD) approach is widely utilized for developing and validating manufacturing processes for drug substances as well as drug products. This paper discusses the application of the risk-based QbD approach used at F. Hoffmann-La Roche Ltd. for development, optimization, and characterization of drug substance manufacturing processes for small molecules. It presents the evolution of the QbD concept into statistical thinking and development of a quantitative tool, namely, the impact ratio concept, for its successful implementation. The utilization of this approach is illustrated with a case study from the taselisib drug substance manufacturing process. more...
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- 2024
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6. Prognostic value of elevated high-sensitivity cardiac troponin T levels in a low risk outpatient population with cardiovascular disease
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Biener, Moritz, Giannitsis, Evangelos, Lamerz, Judith, Mueller-Hennessen, Matthias, Vafaie, Mehrshad, and Katus, Hugo A
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Aims: To investigate the prognostic implications of elevated high-sensitivity cardiac troponin T (hs-cTnT) values in presumably stable ambulatory coronary artery disease patients.Methods and results: We conducted a retrospective, single-centre pilot observational study in a low-risk population. All patients received routine measurement of hs-cTnT at index and follow-up visits. Endpoints were all-cause mortality and a composite of all-cause mortality, acute myocardial infarction, stroke and rehospitalization for acute coronary syndrome and heart failure. Nine hundred and sixty-five consecutive patients presenting to our outpatient clinic between June 2009 and June 2010 were screened; 693 patients with a stable clinical course, at least one hs-cTnT value and at least one follow-up visit qualified for analysis. Follow-up was 796 days. Five hundred and forty-seven patients (78.9%) had hs-cTnT values below and 146 patients (21.1%) had values above 14 ng/l, which was defined to categorize high and low levels as it was reported to be the 99th percentile of a reference population. We observed 13 deaths (all-cause mortality) including four cardiovascular deaths. Age, N terminal pro-brain natriuretic peptide levels and impaired renal function were independently associated with an elevated hs-cTnT in a multivariate analysis. Hs-cTnT values >14 ng/l were strongly associated with all-cause mortality (hazard ratio 12.9, 95% confidence interval (CI): 3.5–46.9, p=0.0001), the composite clinical endpoint (hazard ratio 2.35, 95% CI: 1.48–3.72, p=0.0003) and rehospitalization for heart failure (hazard ratio 3.36, 95% CI: 1.73–6.53, p=0.0004). Compared with the multivariable Framingham score hs-cTnT revealed a significantly better performance (area under the receiver operating characteristics curve (AUC) hs-cTnT: 0.882 vs. AUC Framingham score 0.639, p=0.0005).Conclusion: Elevated hs-cTnT levels provide excellent prognostic information regarding all-cause mortality and a combined clinical endpoint in presumably stable ambulatory coronary artery disease outpatients presenting for routine evaluation. more...
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- 2016
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7. Quantification of HER2 by Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded (FFPE) Breast Cancer Tissues*[S]
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Steiner, Carine, Tille, Jean-Christophe, Lamerz, Jens, Kux van Geijtenbeek, Sabine, McKee, Thomas A., Venturi, Miro, Rubbia-Brandt, Laura, Hochstrasser, Denis, Cutler, Paul, Lescuyer, Pierre, and Ducret, Axel more...
- Abstract
The ability to accurately quantify proteins in formalin-fixed paraffin-embedded tissues using targeted mass spectrometry opens exciting perspectives for biomarker discovery. We have developed and evaluated a selectedreaction monitoring assay for the human receptor tyrosine-protein kinase erbB-2 (HER2) in formalin-fixed paraffin-embedded breast tumors. Peptide candidates were identified using an untargeted mass spectrometry approach in relevant cell lines. A multiplexed assay was developed for the six best candidate peptides and evaluated for linearity, precision and lower limit of quantification. Results showed a linear response over a calibration range of 0.012 to 100 fmol on column (R2: 0.99–1.00).The lower limit of quantification was 0.155 fmol on column for all peptides evaluated. The six HER2 peptides were quantified by selected reaction monitoring in a cohort of 40 archival formalin-fixed paraffin-embedded tumor tissues from women with invasive breast carcinomas, which showed different levels of HER2 gene amplification as assessed by standard methods used in clinical pathology. The amounts of the six HER2 peptides were highly and significantly correlated with each other, indicating that peptide levels can be used as surrogates of protein amounts in formalin-fixed paraffin-embedded tissues. After normalization for sample size, selected reaction monitoring peptide measurements were able to correctly predict 90% of cases based on HER2 amplification as defined by the American Society of Clinical Oncology and College of American Pathologists. In conclusion, the developed assay showed good analytical performance and a high agreement with immunohistochemistry and fluorescence in situhybridization data. This study demonstrated that selected reaction monitoring allows to accurately quantify protein expression in formalin-fixed paraffin-embedded tissues and represents therefore a powerful approach for biomarker discovery studies. The untargeted mass spectrometry data is available via ProteomeXchange whereas the quantification data by selected reaction monitoring is available on the Panorama Public website. more...
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- 2015
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8. E-Books in der Wissenschaft - Fluch oder Segen? Eine Untersuchung der Nutzungsstatistiken der Universitäts- und Landesbibliothek Düsseldorf im Bereich E-Books.
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Lamerz, Lennart
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ELECTRONIC books ,LIBRARIES & electronic publishing ,SCHOLARLY electronic publishing ,ACADEMIC library use studies ,LIBRARY circulation & loans -- Use studies ,LIBRARY statistics ,HEINRICH-Heine-Universitat Dusseldorf. Universitats- und Landesbibliothek ,UNIVERSITIES & colleges - Abstract
Copyright of Information -- Wissenschaft und Praxis is the property of De Gruyter and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
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- 2012
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9. Analysis of promoter methylation in stool: A novel method for the detection of colorectal cancer.
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Lenhard, Konstanze, Bommer, Guido T., Asutay, Silke, Schauer, Rolf, Brabletz, Thomas, Göke, Burkhard, Lamerz, Rolf, and Kolligs, Frank T.
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CANCER patients ,METHYLATION ,STATISTICAL hypothesis testing ,BLOOD testing - Abstract
Background & Aims: Detection of tumor-derived DNA alterations in stool is an intriguing new approach with high potential for the noninvasive detection of colorectal cancer (CRC). Because of heterogeneity of tumors, usually multiple markers distributed throughout the human genome need to be analyzed. This is labor intensive and does not allow for high through-put screening. Therefore, markers with high sensitivity and good specificity are needed. We explored the potential of a single epigenetic marker in comparison with fecal occult blood testing (FOBT) for the discrimination of patients with CRCs and adenomas from those without. Methods: Methylation-specific polymerase chain reaction (PCR) was performed to analyze hypermethylated in cancer 1 (HIC1) promoter methylation status in a blinded fashion in stool samples from 26 patients with CRC, 13 with adenoma ≥1 cm, 9 with hyperplastic polyps, 9 with chronic inflammatory bowel disease, and 32 with endoscopically normal colon. Results: Ninety-seven percent of the stool samples contained amplifiable DNA. Forty-two percent of the samples from patients with CRC and 31% of the samples from patients with colorectal adenoma ≥1 cm were positive for HIC1 promoter methylation. No methylated HIC1 promoter DNA was detected in the fecal DNA from patients with endoscopically normal colon or hyperplastic polyps. Conclusions: The epigenetic marker HIC1 promoter methylation carries high potential for the remote detection of CRCs. We postulate that a panel of merely a few genetic and epigenetic markers will be required for the highly sensitive and specific detection of CRCs and adenomas in fecal samples from affected patients. [Copyright &y& Elsevier] more...
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- 2005
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10. A Novel Immuno-Competitive Capture Mass Spectrometry Strategy for Protein–Protein Interaction Profiling Reveals That LATS Kinases Regulate HCV Replication Through NS5A Phosphorylation*
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Meistermann, Hélène, Gao, Junjun, Golling, Sabrina, Lamerz, Jens, Le Pogam, Sophie, Tzouros, Manuel, Sankabathula, Sailaja, Gruenbaum, Lore, Nájera, Isabel, Langen, Hanno, Klumpp, Klaus, and Augustin, Angélique more...
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Mapping protein–protein interactions is essential to fully characterize the biological function of a protein and improve our understanding of diseases. Affinity purification coupled to mass spectrometry (AP-MS) using selective antibodies against a target protein has been commonly applied to study protein complexes. However, one major limitation is a lack of specificity as a substantial part of the proposed binders is due to nonspecific interactions. Here, we describe an innovative immuno-competitive capture mass spectrometry (ICC-MS) method to allow systematic investigation of protein–protein interactions. ICC-MS markedly increases the specificity of classical immunoprecipitation (IP) by introducing a competition step between free and capturing antibody prior to IP. Instead of comparing only one experimental sample with a control, the methodology generates a 12-concentration antibody competition profile. Label-free quantitation followed by a robust statistical analysis of the data is then used to extract the cellular interactome of a protein of interest and to filter out background proteins. We applied this new approach to specifically map the interactome of hepatitis C virus (HCV) nonstructural protein 5A (NS5A) in a cellular HCV replication system and uncovered eight new NS5A-interacting protein candidates along with two previously validated binding partners. Follow-up biological validation experiments revealed that large tumor suppressor homolog 1 and 2 (LATS1 and LATS2, respectively), two closely related human protein kinases, are novel host kinases responsible for NS5A phosphorylation at a highly conserved position required for optimal HCV genome replication. These results are the first illustration of the value of ICC-MS for the analysis of endogenous protein complexes to identify biologically relevant protein–protein interactions with high specificity. more...
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- 2014
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11. Development of a 5-plex SILAC Method Tuned for the Quantitation of Tyrosine Phosphorylation Dynamics
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Tzouros, Manuel, Golling, Sabrina, Avila, David, Lamerz, Jens, Berrera, Marco, Ebeling, Martin, Langen, Hanno, and Augustin, Angélique
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The propagation of phosphorylation downstream of receptor tyrosine kinases is a key dynamic cellular event involved in signal transduction, which is often deregulated in disease states such as cancer. Probing phosphorylation dynamics is therefore crucial for understanding receptor tyrosine kinases' function and finding ways to inhibit their effects. MS methods combined with metabolic labeling such as stable isotope labeling with amino acids in cell culture (SILAC) have already proven successful in deciphering temporal phosphotyrosine perturbations. However, they are limited in terms of multiplexing, and they also are time consuming, because several experiments need to be performed separately. Here, we introduce an innovative approach based on 5-plex SILAC that allows monitoring of phosphotyrosine signaling perturbations induced by a drug treatment in one single experiment. Using this new labeling strategy specifically tailored for phosphotyrosines, it was possible to generate the time profiles for 318 unique phosphopeptides belonging to 215 proteins from an erlotinib-treated breast cancer cell line model. Hierarchical clustering of the time profiles followed by pathway enrichment analysis highlighted epidermal growth factor receptor (EGFR or ErbB1) and ErbB2 signaling as the major pathways affected by erlotinib, thereby validating the method. Moreover, based on the similarity of its time profile to those of other proteins in the ErbB pathways, the phosphorylation at Tyr453 of protein FAM59A, a recently described adaptor of EGFR, was confirmed as tightly involved in the signaling cascade. The present investigation also demonstrates the remote effect of EGFR inhibition on ErbB3 phosphorylation sites such as Tyr1289 and Tyr1328, as well as a potential feedback effect on Tyr877 of ErbB2. Overall, the 5-plex SILAC is a straightforward approach that extends sample multiplexing and builds up the arsenal of methods for tyrosine phosphorylation dynamics. more...
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- 2013
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12. Multiple Protein Analysis of Formalin-fixed and Paraffin-embedded Tissue Samples with Reverse phase Protein Arrays
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Assadi, Maziar, Lamerz, Jens, Jarutat, Tiantom, Farfsing, Alexandra, Paul, Hubert, Gierke, Berthold, Breitinger, Ewa, Templin, Markus F., Essioux, Laurent, Arbogast, Susanne, Venturi, Miro, Pawlak, Michael, Langen, Hanno, and Schindler, Thomas more...
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Reverse-phase protein arrays (RPPAs) have become an important tool for the sensitive and high-throughput detection of proteins from minute amounts of lysates from cell lines and cryopreserved tissue. The current standard method for tissue preservation in almost all hospitals worldwide is formalin fixation and paraffin embedding, and it would be highly desirable if RPPA could also be applied to formalin-fixed and paraffin embedded (FFPE) tissue. We investigated whether the analysis of FFPE tissue lysates with RPPA would result in biologically meaningful data in two independent studies. In the first study on breast cancer samples, we assessed whether a human epidermal growth factor receptor (HER) 2 score based on immunohistochemistry (IHC) could be reproduced with RPPA. The results showed very good concordance between the IHC and RPPA classifications of HER2 expression. In the second study, we profiled FFPE tumor specimens from patients with adenocarcinoma and squamous cell carcinoma in order to find new markers for differentiating these two subtypes of non-small cell lung cancer. p21-activated kinase 2 could be identified as a new differentiation marker for squamous cell carcinoma. Overall, the results demonstrate the technical feasibility and the merits of RPPA for protein expression profiling in FFPE tissue lysates. more...
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- 2013
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13. Identification of Novel Biomarker Candidates by Differential Peptidomics Analysis of Cerebrospinal Fluid in Alzheimer's Disease
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Selle, Hartmut, Lamerz, Jens, Buerger, Katharina, Dessauer, Andreas, Hager, Klaus, Hampel, Harald, Karl, Johann, Kellmann, Markus, Lannfelt, Lars, Louhija, Jukka, Riepe, Matthias, Rollinger, Wolfgang, Tumani, Hayrettin, Schrader, Michael, and Zucht, Hans-Dieter more...
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The objective of this work was the application of peptidomics®1 technologies for the detection and identification of reliable and robust biomarkers for Alzheimer's disease (AD) contributing to facilitate and further improve the diagnosis of AD. Using a new method for the comprehensive and comparative profiling of peptides, the differential peptide display® (DPD), 312 cerebrospinal fluid (CSF) samples from AD patients, cognitively unimpaired subjects and from patients suffering from other primary dementia disorders were analysed as four independent analytical sets. By combination with a cross validation procedure, candidates were selected from a total of more than 6,000 different peptide signals based on their discriminating power. Twelve candidates were identified using mass-spectrometric techniques as fragments of the possibly neuroprotective neuroendocrine protein VGF and another one as the complement factor C3 descendent C3f. The combination of peptide profiling and cross validation resulted in the detection of novel potential biomarkers with remarkable robustness and a close relation to AD pathophysiology. more...
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- 2005
14. Peptide Sequence Prediction Supported by Correlation-Associated Networks in Human Cerebrospinal Fluid
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Lamerz, Jens, Crameri, Reto, Scapozza, Leonardo, Mohring, Thomas, Selle, Hartmut, and Zucht, Hans-Dieter
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During the course of biosynthesis, processing and degradation of a peptide, many structurally related intermediate peptide products are generated. Human body fluids and tissues contain several thousand peptides that can be profiled by reversed-phase chromatography and subsequent MALDI-ToF-mass spectrometry. Correlation-Associated Peptide Networks (CAN) efficiently detect structural and biological relations of peptides, based on statistical analysis of peptide concentrations. We combined CAN with recognition of probable cleavage sites for peptidases and proteases in cerebrospinal fluid, resulting in a model able to predict the sequence of unknown peptides with high accuracy. On the basis of this approach, identification of peptide coordinates can be prioritized, and a rapid overview of the peptide content of a novel sample source can be obtained. more...
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- 2005
15. Datamining Methodology for LC-MALDI-MS Based Peptide Profiling
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Zucht, Hans-Dieter, Lamerz, Jens, Khamenia, Valery, Schiller, Carsten, Appel, Annette, Tammen, Harald, Crameri, Reto, and Selle, Hartmut
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This report will provide a brief overview of the application of data mining in proteomic peptide profiling used for medical biomarker research. Mass spectrometry based profiling of peptides and proteins is frequently used to distinguish disease from non-disease groups and to monitor and predict drug effects. It has the promising potential to enter clinical laboratories as a general purpose diagnostic tool. Data mining methodologies support biomedical science to manage the vast data sets obtained from these instrumentations. Here we will review the typical workflow of peptide profiling, together with typical data mining methodology. Mass spectrometric experiments in peptidomics raise numerous questions in the fields of signal processing, statistics, experimental design and discriminant analysis. more...
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- 2005
16. 27-jähriger Patient mit rezidivierendem Fieber
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Denk, GeraldUlrich, Bergstermann, Karl-Heinz, Gallmeier, Eike, Schirra, Jörg, Wagner, Hubert, Göke, Burkhard, and Lamerz, Rolf
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Zusammenfassung. Hintergrund: Während die Inzidenz der Tuberkulose in den hoch industrialisierten Ländern rückläufig ist, lässt sich weltweit eine deutliche Zunahme dieser Erkrankung beobachten. In Zeiten zunehmender internationaler Migration gewinnt die frühe Erkennung der Tuberkulose daher auch in Deutschland an Bedeutung. Die Diagnose wird jedoch durch die unspezifische Symptomatik und die nicht immer eindeutige Bildgebung erschwert. Fallbeschreibung: Bei einem 27-jährigen Patienten aus dem Irak mit unklarem rezidivierendem und antibiotikaresistentem Fieber sowie unauffälligem Röntgen- Thorax konnten schließlich mittels Computertomographie im Mediastinum ausgeprägte, nekrotisch zerfallende Lymphknoten festgestellt werden. Nach histologischer Aufarbeitung von mediastinoskopisch gewonnenen Lymphknotenbiopsien konnte eine Mediastinallymphknotentuberkulose nachgewiesen werden. Unter einer testgerechten antituberkulösen Kombinationstherapie kam es zu einer Normalisierung der erhöhten Temperaturen und Beschwerdefreiheit. Ausgehend von diesem Fall werden der Stellenwert der verschiedenen Methoden bei der Tuberkulosediagnostik diskutiert und die Therapie dieser Erkrankung dargestellt. Schlussfolgerung: Einer unklaren fieberhaften Erkrankung auch von jüngeren Patienten kann selbst bei unauffälligem Lungenbefund im konventionellen Röntgenbild eine Tuberkulose zugrunde liegen. Eine frühzeitige Ausweitung der bildgebenden Diagnostik scheint daher sinnvoll. more...
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- 2003
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17. Diagnostik von monoklonalen Gammopathien/Diagnostic Procedures in Monoclonal Gammopathies
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Lamerz, R.
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- 2003
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18. Magnetic resonance imaging as a supplement for the clinical staging system of Durie and Salmon?
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Baur, Andrea, Stäbler, Axel, Nagel, Dorothea, Lamerz, Rolf, Bartl, Reiner, Hiller, Erhard, Wendtner, Clemens, Bachner, Fridleif, and Reiser, Maximilian
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This study evaluated the prognostic value of a three-grade staging system of spinal involvement using magnetic resonance imaging (MRI) in patients with multiple myeloma and determined its usefulness as an independent parameter in the staging system of Durie and Salmon. Seventy-seven previously untreated patients with multiple myeloma underwent MRI of the thoracic and lumbar spine with unenhanced T1-weighted spin echo and short-tau inversion time inversion recovery sequences. The patients were evaluated according to their infiltration patterns and the extent of bone marrow involvement was staged using a three-grade scale: Stage I, no focal or diffuse infiltration; Stage II, 110 foci or mild diffuse infiltration; Stage III, more than 10 foci or strong diffuse infiltration. The infiltration patterns had no significant effect on survival. Of 77 patients, 25 would have been understaged using the standard staging system of Durie and Salmon without the findings of MRI and 8 patients would have been understaged if the staging was based only on MRI. The combination of the staging system of Durie and Salmon and MRI was highly significant with respect to survival (P < 0.0001, log rank analysis). MRI staging IIII was independent of the staging system of Durie and Salmon (Cox regression model). A three-grade staging of spinal MRI provides a significant prognostic tool for patients with multiple myeloma. The authors propose including it in the staging system of Durie and Salmon. Cancer 2002;95:133445. © 2002 American Cancer Society. DOI 10.1002/cncr.10818 more...
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- 2002
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19. Diagnostic significance of carcinoembryonic antigen in the differential diagnosis of malignant mesothelioma
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Mezger, J., Lamerz, R., and Permanetter, W.
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The histologic and cytologic distinction of malignant mesothelioma from carcinomas metastatic to the pleura or peritoneum is often problematic. For this reason immunologic methods are being increasingly used as diagnostic adjuncts. This review summarizes 40 studies on the expression of carcinoembryonic antigen in mesotheliomas and in lung and other carcinomas involving the pleura or peritoneum. Carcinoembryonic antigen was identified immunohistochemically in 11% of mesotheliomas and in 84% of carcinomas examined and immunocytochemically (in serous effusions) in 4% and 58%, respectively. In serum and in pleural or ascitic fluid, significantly elevated levels of carcinoembryonic antigen are commonly associated with (lung) carcinomas but rarely with mesotheliomas. Thus, together with identification of the antigen in serum, pleural fluid, or ascitic fluid, immunohistochemical and immunocytochemical techniques for detecting carcinoembryonic antigen provide a valuable aid for distinguishing malignant mesothelioma from metastatic carcinomas. more...
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- 1990
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20. Epidermal growth factor receptors and epidermal growth factor-like activity in colorectal mucosa, adenomas and carcinomas
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Rothbauer, E., Mann, K., Wiebecke, B., Borlinghaus, P., Lamerz, R., Pratschke, E., Krämling, H. -J., and Pfeiffer, A.
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Summary The epidermal growth factor (EGF) and alpha-tumor growth factor are mitogenic proteins which bind to the EGF-receptor and may play a role in carcinogenesis or tumor progression. Our study investigated whether colorectal carcinomas and adenomas express altered levels of EGF-receptors or overproduce EGF-like activity by comparing histologically normal mucosa to carcinomas resected from the same patients. EGF-receptors were characterized by radioligand binding studies. Carcinomas contained unchanged or decreased levels of EGF-receptors in 13/16 and moderately increased levels in 3/16 patients as compared to normal mucosa. Adenomas obtained from 2 patients with familial polyposis coli and from a third patient with a coincident carcinoma had similar numbers of EGF-receptors as normal mucosa. EGF-like growth factors, in contrast, were significantly elevated in carcinoma extracts as compared to extracts from normal mucosa of the same patients. Adenomas did not contain elevated levels of EGF-like activity. We conclude that increased expression of EGF-receptors is infrequent in colonic adenocarcinomas. Increased production of EGF-like growth factors may frequently occur but seems to be associated with tumor progression rather than with premalignant lesions as represented by adenomas. more...
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- 1989
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21. Carcinofetale Antigene
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Lamerz, R. and Fateh-Moghadam, A.
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Summary The occurrence and significance of important carcinofetal antigens other than AFP and CEA are reported. These include the alpha 2 H-protein which is produced in the liver and increases in serum of patients with various tumors, the fetal sulphoglycoprotein antigen FSA from the gastric juice of patients with gastric cancer, the carcinoplacental alkaline phosphatase (REGAN-isoenzyme) which is found in the serum of patients suffering from e.g. bronchogenic, mammary, urogenital and gastrointestinal carcinomas, the beta-S-fetoprotein which is most likely to be identical with C-reactive protein, gamma-fetoprotein, the carcinofetal antigen in glial tumors (CFGA); ectopic production of placental hormones like human gonadotropin, placental lactogen, plasminogen-activators; leukemia-associated antigens. Furthermore, some other less known carcinofetal antigens are mentioned. more...
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- 1975
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22. Carcinofetale Antigene
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Lamerz, R. and Fateh-Moghadam, A.
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Summary CEA is a beta
1 -glycoprotein (mol. w. approx. 200 000) which in embryonic life is usually found as a cell membrane associated antigen in the gastrointestinal (GI) tract and pancreas. Furthermore, it is secreted into body fluids. In healthy adults a very low serum concentration may be found. The clinical significance of CEA lies in its increased formation in primary and secondary adenocarcinomas of colon and rectum and pancreatic carcinoma, where values of 20 ng/ml and more are observed. However, other gastrointestinal (e.g. oesophagus, stomach, gall-bladder) and extragastrointestinal tumors (e.g. lung, breast, urogenital, prostatic, ovarial carcinomas) as well as non-malignant diseases mainly of the GI tract (e.g. hepatitis, cirrhosis, pancreatitis, colitis, diverticulitis) may provoke less frequent and lower increases in the CEA level. Healthy smokers also tend to show a slight increase in CEA concentration. more...- Published
- 1975
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23. Carcinofetale Antigene
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Lamerz, R. and Fateh-Moghadam, A.
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Summary Alpha-fetoprotein (AFP) is an alpha
1 -glycoprotein (M.W. about 65000) appearing in the fetal serum of most mammals including man during the early stages of pregnancy; 4 weeks after birth in disappears altogether or exists at very low concentrations as in the normal adult. AFP is formed in the yolk sac, the fetal liver and the gastro-intestinal tract. One of its physiological functions in fetal life is supposed to be the protection of the fetus from maternal oestrogens (oestrophilic property). more...- Published
- 1975
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24. Serum-Alpha-Fetoprotein-Veränderungen nach partieller Hepatektomie am Menschen
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Lamerz, R., Grunst, J., Fateh-Moghadam, A., Schmalhorst, U., Pichlmaier, H., and Eisenburg, J.
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Summary Alpha-fetoprotein serum concentrations were determined by radioimmunoassay in 5 patients with echinococcus involvement of the liver. In 4 cases a partial hepatectomy was possible, whereas in 1 case laparotomy had to be confined to an exposure of the liver hilus because of too many dispersed echinococcus cysts. All patients showed normal AFP levels below 20 ng/ml before operation. Within the first to third postoperative day maximal AFP increases of AFP level between 2760 and 5600 ng/ml developed, which returned to normal during the following days and weeks. One patient who was submitted to strumectomy and to the same anaesthetic liver charge had no change in her normal AFP concentration. The postoperative changes of AFP level observed are interpreted as an expression of a quick and intense liver cell regeneration following the surgical treatment. more...
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- 1975
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25. Immunological analysis
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Lüke, F. J., Schlegel, W., Gabor, F., Pittner, F., Linke, R., König, W., Hilgenfeldt, U., Hubl, W., Thiele, H. -J., Meißner, D., Krüger, B., Knoll, M., Gottschall, A., Tessenow, W., Meyer, U., Dummler, W., Cumme, G. A., Ehle, H., Bublitz, R., Horn, A., Drzeniek, Z., Lamerz, R., Haubeck, H. -D., and Wagener, C. more...
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- 1990
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26. Reversible hyperkinesia in a patient with autoimmune polyglandular syndrome type I
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Baumert, T., Kleber, G., Schwarz, J., Stäbler, A., Lamerz, R., and Mann, K.
- Abstract
Autoimmune polyglandular syndrome is characterized by a failure of multiple endocrine organs and the presence of circulating organ-specific autoantibodies targeted against the failing organs. Here we describe a patient with autoimmune polyglandular syndrome type I with the endocrine manifestations of hypoparathyroidism, adrenocortical insufficiency, and insulin-dependent diabetes mellitus. Long-standing hypoparathyroidism led to extensive calcification of the basal ganglia which resulted in the clinical presentation of an extrapyramidal movement disorder (choreoathetotic and hemiballistic hyperkinesia of the left extremities). Interestingly, parallel to rehydration and the initiation of cortisol replacement therapy a complete reversion of the hyperkinetic signs was achieved. This case shows a rare multiendocrine organ failure with complex metabolic interactions resulting in marked neurological signs. Furthermore, this case demonstrates for the first time that a hyperkinetic syndrome - most likely due to hypoparathyroidism-induced basal ganglia calcification - can be reversed solely by adequate treatment of the concomitant endocrine failures. more...
- Published
- 1993
- Full Text
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27. Epidermal growth factor, transforming growth factor-α, and epidermal growth factor receptor content in normal and carcinomatous gastric and colonic tissue
- Author
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Borlinghaus, P., Wieser, S., and Lamerz, R.
- Abstract
Epidermal growth factor (EGF) and transforming growth factor-a (TGF-a) are polypeptides which bind to the EGF receptor (EGFr) and may play a role in cell growth and carcinogenesis. Our study investigated the content of EGF, TGF-a, and EGFr in tumors of the stomach and the colon in comparison with the sourrounding mucosa. EGF was detected in half of the stomach specimens with concentrations between 1 and 9 ng/g weight irrespective of histology. In the colon no EGF was found in the tumor or normal mucosa. In the stomach normal mucosa contained higher TGF-a concentrations (mean 22.4 ng/g) than the tumors (mean 11.8 ng/g), but the difference was not statistically significant because of a wide variation in mucosal values. By contrast, the colon mucosa displayed significantly higher TGF-a concentrations than the tumor tissues (33 ng/g versus 12 ng/g; P < 0.01). EGFr content in the gastric mucosa was lower compared to gastric carcinoma (48 fmol/g versus 75 fmol/g) yet not significantly different. In contrast, colorectal tumor specimens disclosed significantly higher concentrations than the mucosal tissues (mean of 155 fmol/g versus 80 fmol/g; P < 0.01). In conclusion, TGF-a should not be considered a tumorigenic but a physiological growth factor in the stomach and colon. An elevated EGFr content in colorectal tumors in comparison with the normal mucosa could lead to a growth advantage by an autostimulating mechanism. more...
- Published
- 1993
- Full Text
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28. Prognostic factors in multiple myeloma: role of β2-microglobulin and thymidine kinase
- Author
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Diem, H., Fateh-Moghadam, A., and Lamerz, R.
- Abstract
Serum ß
2 - microglobulin, serum thymidine kinase, and commonly used prognostic parameters were investigated for their prognostic value in a well-defined group of patients with multiple myeloma (n = 207). Multivariate analysis showed hemoglobin to be the parameter of strongest prognostic value. Only albumin, serum ß2 -microglobulin and serum thymidine kinase added further prognostic information. When tested for efficiency in recognizing patients with poor (average survival time < 1 year) and good (average survival time > 5 years) prognosis, serum ß2 -microglobulin was best (80%), followed by total urinary protein (78%), hemoglobin (76%), and albumin (75%). more...- Published
- 1993
- Full Text
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29. CEA Determination in the Follow-Up of Extracolorectal Neoplasms
- Author
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Lamerz, R.
- Abstract
CEA was initially described as a tumor and organ specific colorectal antigen, but later found by more sensitive methods in other tumors (stomach, pancreas, lung, breast) and in minor amounts in inflammatory, normal adult and fetal organs of the gastrointestinal tract. The main clinical application of CEA concerns its pretherapeutic and serial determination as circulating antigen in serum and other body fluids by means of CEA-specific, commercially available test kits. By clinical studies a significant correlation has been proven between the pretherapeutic serum CEA level and tumor stages and prognosis. Moreover, serial CEA level changes have been shown a valuable monitor following operation or during radio/chemotherapy anticipating and reflecting the clinical course of disease. In combination with newly established tumor markers, the main clinical indication for CEA determination in addition to colorectal cancer concerns monitoring of patients with stomach (+ CA 72-4), lung (+ NSE/SCC) and breast cancer (+ CA 15-3/MCA). more...
- Published
- 1992
- Full Text
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30. Cisplatin‐based chemotherapy of primary extragonadal germ cell tumors: A single institution experience
- Author
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Gerl, Arthur, Clemm, Christoph, Lamerz, Rolf, and Wilmanns, Wolfgang
- Abstract
Extragonadal germ cell tumors account for only 2‐5% of all germ cell neoplasms in adult males. Because these tumors are rare and, in part, biologically distinct from their testicular counterparts, their optimal management continues to be defined. The medical records of 51 patients with extragonadal germ cell tumors were reviewed. All patients were treated with cisplatin‐based chemotherapy at a single institution between 1981 and 1994. Thirty‐five patients had nonseminomatous germ cell tumors and 16 had pure seminomas. Sixteen tumors arose in the mediastinum (12 nonseminomas, 4 seminomas), and 35 in the retroperitoneum (23 nonseminomas, 12 seminomas). Six of 12 patients (50%) with mediastinal nonseminomas survived with no evidence of disease (NED) at 33‐137 months (median, 96 months); all had undergone surgery as part of their treatment. Fifteen of 23 patients (65%) with retroperitoneal nonseminomas are alive with NED at 2‐145 months (median, 39 months). Fifteen of 16 patients (94%) with extragonadal seminomas survived with NED at 2‐141 months (median, 66 months), and 1 patient died from late irradiation‐related toxicity. Three patients with retroperitoneal nonseminomas developed a testicular seminoma at 35, 42, and 77 months, respectively; all are currently disease free. Mediastinal and retroperitoneal nonseminomas have distinct clinical features. As in other series, clinical outcome is somewhat inferior for mediastinal nonseminomas compared with retroperitoneal nonseminomas. Regardless of the site of presentation, the vast majority of patients with extragonadal seminomas can expect cure. It remains controversial, however, whether retroperitoneal germ cell tumors are metastatic from a primary testicular germ cell tumor. Cancer 1996;77:526‐32. more...
- Published
- 1996
- Full Text
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31. Quantitatives Verhalten der Immunglobuline bei Hodgkin-Patienten nach Splenektomie
- Author
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Steidle, Christoph, Fateh-Moghadam, Ahmad, Lamerz, Rolf, and Huhn, Dieter
- Abstract
Zusammenfassung Es wurden bei 14 Patienten mit M. Hodgkin und einem Patienten mit großfollikulärem Lymphoblastom die Immunglobuline vor und 1 bis 44 Wochen nach Splenektomie quantitativ kontrolliert. Während in der IgG- und IgA-Fraktion keine signifikante Konzentrationsänderung feststellbar war, trat in der IgM-Fraktion in allen Fällen ein signifikanter Abfall auf. Die Ursache dieser IgM-Verminderung ist am ehesten Folge des Fortfalls IgM-produzierender Zellen in der Milz. more...
- Published
- 1975
- Full Text
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32. Prognosis after salvage treatment for unselected male patients with germ cell tumours
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Gerl, A, Clemm, C, Schmeller, N, Hartenstein, R, Lamerz, R, and Wilmanns, W
- Abstract
Long-term outcome of salvage treatment was reviewed in 67 unselected male patients relapsing during or after their primary cisplatin-based chemotherapy for metastatic germ cell tumours. Seven patients underwent only surgery and/or radiotherapy as curatively intended salvage treatment. Thirty-five patients (52%) had a complete or partial response to salvage treatment, 20 (57%) of whom relapsed again. With a median follow-up of 90 months (range 3-143 months) 20 patients (30%) are alive with no evidence of disease, 15 continuously disease-free and five currently disease-free. The 5 year survival from start of salvage treatment is 37% for the group as a whole. Multivariate analysis identified age < or = 35 years, complete response to primary treatment and a relapse-free interval > 3 months as independent predictors of favourable outcome of salvage treatment. A group of patients with these good-risk factors (42%) had a 5 year survival of 72% compared with the remaining patients (58%) with a 5 year survival of only 11%. Whereas patients with good-risk features may be adequately managed by conventional salvage treatment, the remaining patients carry a very poor prognosis and require innovative and more aggressive approaches. more...
- Published
- 1995
- Full Text
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33. Immunological comparison of carcinoembryonic antigen (CEA) extracted from tumours of various organs: Their use in radioimmunological CEA determinations
- Author
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Lamerz, R and Burtin, P
- Abstract
CEA was extracted by the perchloric acid method from primary adenocarcinomas of the colon and the ovary, from ascitic and pleural fluids from patients with pancreatic, lung and breast cancer, and from the cyst fluid of a benign ovarian cystadenoma. Further purification included gel filtration and affinity chromatography. Antisera against CEA from colon, breast, ovary, lung and pancreatic cancer were produced in rabbits. In double diffusion experiments, all these CEA samples showed a reaction of complete immunological identity with all the anti-CEA sera, whatever their origin. CEA from colon, breast, pancreas and ovary were labelled with 125I and used in radioimmunological experiments. In a radioimmunological system where the tracer and the antiserum were constant, all the CEA used as standards gave parallel inhibition curves, having nearly identical slopes. This was another criterion of immunological identity. Sera of numerous cancer patients were assayed in several RIA systems, one of them being the classical system with colonic CEA as tracer and anti-colonic CEA as antiserum, the others being "organ specific" systems. The values obtained in these assays were found to be highly correlated: the rank coefficient of correlation between colonic and breast cancer RIA systems was rs = 0.96, that between colonic and ovarian RIA systems, 0.92, that between colonic and pancreatic RIA systems, 0.97 and that between colonic and lung RIA systems 0.96. It is thus concluded that by use of different organ-derived CEA preparations and their corresponding polyclonal antisera, no significative differences in serum CEA levels may be expected. No evidence of organ specificity of serum CEA was found. more...
- Published
- 1983
- Full Text
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34. MRI gadolinium enhancement of bone marrow: age-related changes in normals and in diffuse neoplastic infiltration
- Author
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Baur, A., Stäbler, A., Bartl, R., Lamerz, R., Scheidler, J., and Reiser, M.
- Abstract
Abstract: Objective: To quantify gadolinium-related enhancement in the bone marrow of the spine in normals and in patients with homogeneous diffuse malignant bone marrow infiltration. Design and patients: The patients consisted of two groups: group 1 comprised 94 healthy adults (18–86 years) without bone marrow disease and group 2 comprised 30 patients with homogeneous diffuse malignant bone marrow infiltration due to myeloma (n=20) or breast carcinoma (n=10). All patients received intravenous gadopentetate dimeglumine (Gd-DTPA), 0.1 mmol/kg body weight. Pre- and postcontrast signal intensity (SI) on T1-weighted spin-echo (SE) images (TR/TE: 572 ms/15 ms) was measured over a region of interest (ROI) and the percentage SI increase was calculated. The results were confirmed by bone marrow biopsy (n=20) and clinical parameters (n=10). Dynamic contrast-enhanced studies using a spoiled gradient-recalled-echo (GRE) sequence (TR/TE/α: 68 ms/6 ms 75) were performed in 10 controls with normal bone marrow. Results and conclusion: Contrast material enhancement in healthy persons can vary greatly (range 3–59%, mean 21%, SD 11%). With increasing age there is a significant decrease in contrast enhancement (Pearson’s correlation, P<0.01). The percentage SI increase in patients with intermediate-grade (biopsy 20–50 vol%) and high-grade (biopsy >50 vol%) diffuse malignant bone marrow infiltration was significantly higher than in normals (mean 67%, SD 34%, P<0.001). Low-grade (biopsy <20 vol%) diffuse malignant bone marrow infiltration can not be assessed by non-enhanced T1-weighted SE images or Gd-DTPA application. In conclusion, contrast material enhancement in healthy persons can vary greatly and is dependent on age, while intermediate-grade and high-grade diffuse malignant bone marrow infiltration can be objectively assessed with SI measurements. more...
- Published
- 1997
- Full Text
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35. Light chain deposition disease in multiple myeloma: MR imaging features correlated with histopathological findings
- Author
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Baur, A., Stäbler, A., Lamerz, R., Bartl, R., and Reiser, M.
- Abstract
Abstract: The clinical, histopathological, and imaging findings on MRI of a 56-year-old woman with light chain deposition disease occurring in multiple myeloma are presented. Light chain deposition disease is a variant of multiple myeloma with distinct clinical and histological characteristics. MRI of this patient also revealed an infiltration pattern in the bone marrow distinct from that of typical multiple myeloma. Multiple small foci of low signal intensity were present on T1- and T2-weighted spin echo and STIR images, corresponding to conglomerates of light chains in bone marrow biopsy. Contrast-enhanced T1-weighted spin echo images show diffuse enhancement of 51% over all vertebral bodies, with a minor enhancement of the focal conglomerates of light chains. Light chain deposition disease in multiple myeloma should be added to the list of those few entities with normal radiographs and discrete low-signal marrow lesions on T1- and T2-weighted spin echo pulse sequences. more...
- Published
- 1998
- Full Text
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36. Advances in the management of metastatic non-seminomatous germ cell tumours during the cisplatin era: a single-institution experience
- Author
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Gerl, A, Clemm, C, Schmeller, N, Hartenstein, R, Lamerz, R, and Wilmanns, W
- Abstract
Long-term outcome was reviewed in 266 consecutive patients with metastatic non-seminomatous germ cell tumours treated at a single institution. The overall 3 year survival was 77%, and 3 year progression-free survival was 71%. Multivariate analysis identified the following clinical features as independent prognostic factors: the presence of liver, bone or brain metastasis, serum human chorionic gonadotropin > or = 10000 U l-1 and/or alpha-fetoprotein > or = 1000 ng ml-1, a mediastinal mass > 5 cm and the presence of 20 or more lung metastases. Age was not of prognostic significance. Patients without any of the above poor-risk factors had a 3 year survival of 91% regardless of etoposide- or vinblastine-containing chemotherapy compared with 61% for the remaining patients. However, etoposide-containing protocols led to significantly improved survival in patients with at least one poor risk factor. After 612 patient-years of observation no case of secondary leukaemia was observed among 119 surviving patients who had received etoposide as part of their treatment. With a median follow-up of 93 months, five patients developed a second germ cell tumour, two patients nongerm cell malignancies. Fourteen patients relapsed after a disease-free interval of more than 2 years, and nine patients died more than 5 years after commencement of treatment underscoring the need to report long-term results. There is some evidence that cumulative experience translates into improved survival and cure rates for patients with poor-risk metastatic disease. more...
- Published
- 1996
- Full Text
- View/download PDF
37. Radioimmunologische Bestimmung von Immunglobulin E
- Author
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Lamerz, R. and Fateh-Moghadam, A.
- Abstract
Summary By means of a commercial solid-phase radioimmuno IgE-test and the evaluation by a desk-computer-program, 36 IgE-tests—corresponding to 1800 determinations—were evaluated through the logit-log-method and a linear regression curve. Each conversion curve showed a good correlation coefficient ofr>|0.99| in 5–7 correlated pairs and similar parametersA (ordinate intercept) andB (slope). The IgE values of each of the 6 disease groups and one control group—checked by this method—showed an approximate Gaussian distribution only after logarithmic transformation. In comparison with controls, the patients with parasitic diseases and atopic eczema showed the significantly highest IgE-levels in serum. The IgE-increase was less pronounced in patients with extrinsic and intrinsic asthma, as well as those with clinically presumed atopic diathesis. Patients with chronic nephropathies and those after kidney transplantation did not differ from the controls, whereas the ones with various paraproteinemias showed a significant IgE-decrease. more...
- Published
- 1974
- Full Text
- View/download PDF
38. Immunglobulin E
- Author
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Lamerz, R. and Fateh-Moghadam, A.
- Abstract
Summary Immunoglobulin E represents a heat-sensitive glycoproteid which consists of two class-specific heavy (epsilon) and 2 light polypeptide chains. Biologically it is capable of tissue fixation and sensitization in man (Prausnitz-Küstner reaction) and animal (passive cutaneous anaphylaxy). After fixation of IgE through its Fc-portion to tissue mast cells or basophile granulocytes, an activation of enzyme systems is initiated through the reaction with a specific antigen (allergen, atopen), or directly by anti-IgE-antibody leading to the liberation of vasoactive substances, which correlates with clinical allergies of the immediate type in the skin and mucosa. IgE is produced preferentially by plasma cells in the tonsils and adenoids, in bronchial and peritoneal lymph nodes, in respiratory, gastrointestinal and nasal mucosa. Unbound IgE can be found in serum, urine, sputum, nasal and bronchial secretions and in tears as well as in colostrum, but does not pass through the placenta. The normal average serum concentration in adults corresponds to 100–500 ng/ml and 80–200 U/ml respectively. Increased levels are mainly encountered in allergic (atopic) diseases (asthma, hay fever), atopic eczema (Neurodermitis diffusa) and some parasitic infections. Decreased levels were reported in various cases of hypo/agammaglobulinemia, tumours and other diseases. more...
- Published
- 1974
- Full Text
- View/download PDF
39. Zur quantitativen immunologischen Bestimmung von Serumproteinen
- Author
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LAMERZ, R., FATEH-MOGHADAM, A., and KNEDEL, M.
- Published
- 1973
- Full Text
- View/download PDF
40. Elektrophoretische Klassen- und Gruppenverteilung von „Paraproteinen“ und normalen Immunglobulinen
- Author
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Fateh-Moghadam, A., Lamerz, R., Knedel, M., and Tsirimbas, B.
- Abstract
Summary In 962 paraproteinemias the class distribution revealed the following data: IgG paraproteinemia 627 cases (65%), IgA paraproteinemia 175 cases (18%), IgM paraproteinemia 118 cases (13%), double paraproteinemia 13 cases (1%), Bence Jones plasmacytoma 29 cases (3%). With single respect to the main classes IgG, IgA and IgM, the percentage changes to 68%, 19% and 13% respectively. By this the frequency distribution of the main paraproteinemia classes equals the relative serum concentration of the corresponding normal immunoglobulins of an age-adequate normal collective. The light chain type was determined in 717 paraproteins. The followingx/? ratios were found: total of paraproteins 1.7:1, IgG paraproteins 1.7:1, IgA p. 1:1, IgM p. 4:1, micromolecular p. 1.7:1 and double p. 2.5:1. These ratios agree with the figures of normal immunoglobulines except for the IgM paraproteins. There is a significant difference between thex/? ratio of IgM paraproteins and paraproteins of other classes. Following statistical analysis, the electrophoretical frequency distribution of our IgM and IgG paraproteins as well as of a pool of normal IgG and IgM immunogloublins are better represented by a fitted normal distribution curve of second mode (asymmetrical normal distribution curve). In comparison to this the IgA paraproteins and corresponding normal immunoglobulins are more similary distributed following a normal Gaussian distribution curve of first mode (symmetrical d. c.). Like normal immunoglobulins, the electrophoretical distribution of paraproteins extends from a
2 to the slow? range. The fitted electrophoretical frequency distribution of paraproteins reveals an obvious similarity with that of the corresponding normal immunoglobulins. By severe statistical analysis, however, there was a significant difference between the non-fitted distribution curves of IgG and IgA paraproteins and the normal IgG and IgA immunoglobulins, respectively, in contrast to the distribution curves of IgM paraproteins and IgM normal immunoglobulins. No correlation was found between the light chain type of paraprotein and its electrophoretical mobility or sedimentation characteristics. more...- Published
- 1971
- Full Text
- View/download PDF
41. Das Serumgruppen-System Gc, Gm und InV bei Paraproteinämien
- Author
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Knedel, M., Fateh-Moghadam, A., Lamerz, R., and Maiss, C.
- Abstract
Summary Gc, Gm, and InV factors were determined in patients with paraproteinaemia by means of electrophoresis and the hemagglutination inhibition technique. The distribution of Gc, Gm, and InV factors was compared with the corresponding normal values. In contrast to various other diseases which bear a statistical relation to the AB0 blood group and other serum group systems, no such correlation could be demonstrated in patients with paraproteinaemic diseases (plasmacytoma and Morbus Waldenstroem), nor were there any statistically significant differences between the paraproteinaemic classes IgG, IgA, and IgM with respect to Gc, Gm, and InV frequencies. The factors were also equally distributed as regards the light chain types ? and ?. more...
- Published
- 1971
- Full Text
- View/download PDF
42. Immunglobulinvermehrung und Antikörperbildung unter Asparaginase-Therapie
- Author
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Lamerz, R., Fateh-Moghadam, A., Hornung, B., and Ehrhart, H.
- Abstract
Summary 1. In 12 patients with acute and chronic leukemia and solid tumours—among them 4 individuals with symptomatic antibody deficiency syndrome (ADS)—a reversible increase of serum immunoglobulins IgG, IgA and IgM could be observed under therapy with E. coli L-asparaginase. By contrast to these findings, this effect could not be realized in two patients with an idiopathic and a severe symptomatic ADS combined with IgG-paraproteinemia, respectively, under asparaginase treatment of lower dosage. more...
- Published
- 1971
- Full Text
- View/download PDF
43. Die Bedeutung der Immunglobuline für die Diagnose und Verlaufsbeurteilung von Lebererkrankungen
- Author
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Fateh-Moghadam, A., Lamerz, R., Eisenburg, J., and Knedel, M.
- Abstract
Zusammenfassung Mit der radialen Immunodiffusionsmethode nachMancini wurden die IgG-, IgA-und IgM-Konzentrationen bei 55 Patienten mit verschiedenen Lebererkrankungen bestimmt. Die dabei erhaltenen Ergebnisse sind in Tabelle 4 zusammengestellt. more...
- Published
- 1969
- Full Text
- View/download PDF
44. Alloalbuminämie (Doppelalbuminämie)
- Author
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Fateh-Moghadam, A., Eisenburg, J., and Lamerz, R.
- Abstract
Zusammenfassung Es wird über das Auftreten von Doppelalbuminämie in einer Sippe berichtet. Von 54 noch lebenden blutsverwandten Mitgliedern wurden 31 untersucht. Davon wiesen 17 (10 weibliche, 7 männliche) den Befund einer erbbedingten Doppelalbuminämie auf. Der Doppelalbuminämiebefund konnte mittels Acetatfolienelektrophorese-Untersuchung der Seren erhoben und als zum langsamen Typ gehörig gesichert werden. Die Papierelektrophorese-Untersuchung erbrachte keine eindeutige Trennung der beiden Albuminkomponenten, die in der CAF-Elektrophorese ein Flächenverhältnis von 6/5 (normalschnell/langsam wandernde Albuminkomponente) aufwiesen. In der Immunelektrophorese-Untersuchung zeigte sich auch bei Verwendung von Serumverdünnungen und im Ansatz gegen verschiedene Antihumanseren und monospezifische Antialbuminseren stets nur eine typische symmetrisch gebogene Form der Albuminpräcipitationslinie. Beide Albuminkomponenten konnten mittels kombinierter präparativer Kartonelektrophorese und Säulenchromatographie jeweils in elektrophoretisch, immunelektrophoretisch und sedimentationsanalytisch reiner Form gewonnen werden. Sie zeigten ein annähernd gleich großes S
20W um 4,5 und eine immunologische Identitätsreaktion. more...- Published
- 1969
- Full Text
- View/download PDF
45. Serumproteinveränderungen bei chronischer Azotämie und nach Nierentransplantation
- Author
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Fateh-Moghadam, A., Edel, H. H., Lamerz, R., and Schneemilch, K.
- Abstract
Summary The concentrations of the serum proteins IgA, IgG, IgM, a
2 -haptoglobin, ß1 A-globulin, coeruloplasmin, transferrin and albumin were evaluated in 39 azotaemic patients; the modifications in concentration were also assessed in 17 patients after kidney transplantation. All 280 sera were subjected to electrophoresis and immunoelectrophoresis. more...- Published
- 1973
- Full Text
- View/download PDF
46. Darstellung eines lipolytisch wirksamen Polypeptids (Peptid A) aus Hypophysen vom Schwein
- Author
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Schwandt, P., Weisweiler, P., and Lamerz, R.
- Abstract
Zusammenfassung: Aus Hypophysen vom Schwein wurde eine als Peptid A bezeichnete Substanz dargestellt, die sich unter den angegebenen Bedingungen als einheitlich in Sulenchromatographie, Acetatfolien- und Acrylamidgelelektrophorese, Ultrazentrifuge sowie im Immunodiffusionstest erwies. Das mit der Ultrazentrifuge bestimmte Molekulargewicht lag bei 5700. Die minimal wirksame Dosis betrug bei Kaninchen in vitro 0,1 Μg/ml. Nach den vorliegenden Untersuchungen ist anzunehmen, da Peptid A von MSH, ACTH, STH und TSH verschieden ist. more...
- Published
- 1971
- Full Text
- View/download PDF
47. Quantitative Veränderungen der Serumproteine und -lipide im Verlauf der Asparaginase-Therapie maligner Tumoren und Hämoblastosen
- Author
-
Fateh-Moghadam, A., Lamerz, R., Schwandt, P., Hornung, B., and Ehrhart, H.
- Abstract
Zusammenfassung Es wird über Konzentrationsänderungen von 9 Serumproteinen und 4 -lipidfraktionen bei 8 Patienten mit malignen Tumoren und Hämoblastosen berichtet, die mit L-Asparaginase über einen Zeitraum zwischen 14 und 36 Tagen behandelt wurden. Unter Asparaginase-Therapie konnte bei nahezu allen Patienten ein Absinken der Ausgangskonzentration von Albumin, a
2 -Makroglobulin, Coeruloplasmin, a1 -Antitrypsin, Transferrin und a2 -Haptoglobin beobachtet werden, während diese Proteine nach Absetzen der Therapie eine Tendenz zur Rückkehr zu den Ausgangskonzentrationen erkennen ließen. Für die Immunglobuline konnte ein gegenläufiges Verhalten gefunden werden, d. h. Zunahme von IgG, IgA und IgM unter Asparaginase-Therapie und eine allmähliche Abnahme nach Absetzen der Therapie. Bei den Serumlipiden fanden sich folgende Veränderungen: die freien Fettsäuren stiegen während der Behandlung an, die Gesamtlipide, Triglyceride und das Cholesterin fielen innerhalb der ersten 14 Behandlungstage ab. Nach Absetzen der Therapie zeigte sich bei den nachuntersuchten Fällen eine Normalisierungstendenz. more...- Published
- 1970
- Full Text
- View/download PDF
48. Buchbesprechungen
- Author
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Schwand, P., Thoenes, G. H., Burkhardt, R., Lamerz, R., Siess, M., Schierz, G., Buchborn, E., and Gsell, O.
- Published
- 1976
- Full Text
- View/download PDF
49. Hepatitis a virus infection of HBsAg-producing hepatocellular carcinomas in athymic mice
- Author
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Gauss-Müller, Verena, Deinhardt, Friedrich, Hahn, Eckhart, and Lamerz, Rolf
- Abstract
Two human hepatocellular carcinoma cell lines were grown in athymic mice. Morphologically, the tumors resembled hepatocellular carcinomas. HBsAg, anti-HBs, and a-fetoprotein were detected in sera of mice bearing tumors of PLC/PRF/5 or Hep 3B cells; their amounts correlated with tumor weight. Tumors of both cell lines were infected with cell culture-adapted hepatitis A virus (HAV); HAV antigen and infectious virus were recovered from infected tumors of PLC/PRF/5 and Hep 3B cells. Our results indicate that HAV-infected tumors may be useful in studying the production in vivo of hepatitis A virus antigen. more...
- Published
- 1982
- Full Text
- View/download PDF
50. Buchbesprechungen
- Author
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Stich, W., Schwandt, P., Ruhenstroth-Bauer, G., Huhn, D., Witte, S., Eulitz, M., and Lamerz, R.
- Published
- 1972
- Full Text
- View/download PDF
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