241 results on '"Xiao-Peng, He"'
Search Results
2. A Highly Sensitive and Selective Near-Infrared Fluorescent Probe for Imaging Peroxynitrite in Living Cells and Drug-Induced Liver Injury Mice
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Xianzhi Chai, Bohan Li, Chi Chen, Weijian Zhang, Lulu Sun, Hai-Hao Han, Yafei Zhang, Shasha Sun, Jianming Yang, Junji Zhang, and Xiao-Peng He
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Analytical Chemistry - Published
- 2023
3. The design of small-molecule prodrugs and activatable phototherapeutics for cancer therapy
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Hai-Hao Han, Han-Min Wang, Paramesh Jangili, Mingle Li, Luling Wu, Yi Zang, Adam C. Sedgwick, Jia Li, Xiao-Peng He, Tony D. James, and Jong Seung Kim
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General Chemistry - Abstract
This tutorial review provides a general overview for the design of prodrugs and activatable phototherapeutics which enables the development of improved therapies.
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- 2023
4. Targeted photothermal release of antibiotics by a graphene nanoribbon-based supramolecular glycomaterial
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Ying Shang, Sheng Zhang, Hui-Qi Gan, Kai-Cheng Yan, Fugui Xu, Yiyong Mai, Daijie Chen, Xi-Le Hu, Lei Zou, Tony D. James, and Xiao-Peng He
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Materials Chemistry ,Metals and Alloys ,Ceramics and Composites ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Abstract
A supramolecular glycomaterial has been developed using the self-assembly of pyrenyl glycosides and graphene nanoribbon-based nanowires for the targeted, photothermally-controlled release of antibiotics to bacteria.
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- 2023
5. Fluorescent probes for the detection of chemical warfare agents
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Wen-Qi Meng, Adam C. Sedgwick, Nahyun Kwon, Mingxue Sun, Kai Xiao, Xiao-Peng He, Eric V. Anslyn, Tony D. James, and Juyoung Yoon
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General Chemistry - Abstract
Chemical warfare agents (CWAs) are toxic chemicals that have been intentionally developed for targeted and deadly use on humans. Although intended for military targets, the use of CWAs more often than not results in mass civilian casualties. To prevent further atrocities from occurring during conflicts, a global ban was implemented through the chemical weapons convention, with the aim of eliminating the development, stockpiling, and use of CWAs. Unfortunately, because of their relatively low cost, ease of manufacture and effectiveness on mass populations, CWAs still exist in today's world. CWAs have been used in several recent terrorist-related incidents and conflicts (
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- 2023
6. Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore
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Yueci Wu, Hai-Hao Han, Liu He, Li Li, Yi Zang, Jia Li, Xiao-Peng He, Yaping Ding, Weiguo Cao, and Tony D. James
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Materials Chemistry ,Metals and Alloys ,Ceramics and Composites ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Abstract
A turn-on isatin receptor based naphthalimide fluorescent probe encapsulated in PEG was used to rapidly detect peroxynitrite in cells.
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- 2023
7. Metabolically Specific In Situ Fluorescent Visualization of Bacterial Infection on Wound Tissues
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Chen-Jian Zhong, Xi-Le Hu, Xiao-Lan Yang, Hui-Qi Gan, Kai-Cheng Yan, Fu-Ting Shu, Pei Wei, Teng Gong, Peng-Fei Luo, Tony D. James, Zhao-Hong Chen, Yong-Jun Zheng, Xiao-Peng He, and Zhao-Fan Xia
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General Materials Science - Published
- 2022
8. Welcome to Fluorescent Chemosensors
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Luling Wu, Adam C. Sedgwick, Xiao-Peng He, and Tony D. James
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We would like to welcome you to the wonderful world of fluorescent chemosensors. This book consists of 16 chapters that take you on a journey from receptor-based to reaction-based sensing systems. The quest includes both molecular-based and nanoparticle-based systems that can be used for medical diagnostics, theranostics and environmental sensing applications. The techniques included embrace two-photon fluorescence, ratiometric and aggregation-induced emission.
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- 2023
9. A homogeneous high-throughput array for the detection and discrimination of influenza A viruses
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Wei-Tao Dou, Xiang Wang, Tingting Liu, Suwen Zhao, Jiao-Jiao Liu, Yao Yan, Jun Li, Chi-Yu Zhang, Adam C. Sedgwick, He Tian, Jonathan L. Sessler, Dong-Ming Zhou, and Xiao-Peng He
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General Chemical Engineering ,Biochemistry (medical) ,Materials Chemistry ,Environmental Chemistry ,General Chemistry ,Biochemistry - Published
- 2022
10. Fluorescent probes and functional materials for biomedical applications
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Xi-Le Hu, Hui-Qi Gan, Fan-De Meng, Hai-Hao Han, De-Tai Shi, Shu Zhang, Lei Zou, Xiao-Peng He, and Tony D. James
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General Chemical Engineering - Abstract
Due to their simplicity in preparation, sensitivity and selectivity, fluorescent probes have become the analytical tool of choice in a wide range of research and industrial fields, facilitating the rapid detection of chemical substances of interest as well as the study of important physiological and pathological processes at the cellular level. In addition, many long-wavelength fluorescent probes developed have also proven applicable for in vivo biomedical applications including fluorescence-guided disease diagnosis and theranostics (e.g., fluorogenic prodrugs). Impressive progresses have been made in the development of sensing agents and materials for the detection of ions, organic small molecules, and biomacromolecules including enzymes, DNAs/RNAs, lipids, and carbohydrates that play crucial roles in biological and disease-relevant events. Here, we highlight examples of fluorescent probes and functional materials for biological applications selected from the special issues “Fluorescent Probes” and “Molecular Sensors and Logic Gates” recently published in this journal, offering insights into the future development of powerful fluorescence-based chemical tools for basic biological studies and clinical translation.
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- 2022
11. Fluorescence Analysis of Circulating Exosomes for Breast Cancer Diagnosis Using a Sensor Array and Deep Learning
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Yuyao Jin, Nan Du, Yuanfang Huang, Wanxiang Shen, Ying Tan, Yu Zong Chen, Wei-Tao Dou, Xiao-Peng He, Zijian Yang, Naihan Xu, and Chunyan Tan
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Fluid Flow and Transfer Processes ,Deep Learning ,Process Chemistry and Technology ,Liquid Biopsy ,Humans ,Breast Neoplasms ,Female ,Bioengineering ,Exosomes ,Instrumentation ,Fluorescent Dyes - Abstract
Emerging liquid biopsy methods for investigating biomarkers in bodily fluids such as blood, saliva, or urine can be used to perform noninvasive cancer detection. However, the complexity and heterogeneity of exosomes require improved methods to achieve the desired sensitivity and accuracy. Herein, we report our study on developing a breast cancer liquid biopsy system, including a fluorescence sensor array and deep learning (DL) tool AggMapNet. In particular, we used a 12-unit sensor array composed of conjugated polyelectrolytes, fluorophore-labeled peptides, and monosaccharides or glycans to collect fluorescence signals from cells and exosomes. Linear discriminant analysis (LDA) processed the fluorescence spectral data of cells and cell-derived exosomes, demonstrating successful discrimination between normal and different cancerous cells and 100% accurate classification of different BC cells. For heterogeneous plasma-derived exosome analysis, CNN-based DL tool AggMapNet was applied to transform the unordered fluorescence spectra into feature maps (Fmaps), which gave a straightforward visual demonstration of the difference between healthy donors and BC patients with 100% prediction accuracy. Our work indicates that our fluorescent sensor array and DL model can be used as a promising noninvasive method for BC diagnosis.
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- 2022
12. QTL mapping for plant architecture in rice based on chromosome segment substitution lines
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Xiao-Lei WANG, Wei-Xing LI, Lin-Juan OU-YANG, Jie XU, Xiao-Rong CHEN, Jian-Min BIAN, Li-Fang HU, Xiao-Song PENG, Xiao-Peng HE, Jun-Ru FU, Da-Hu ZHOU, Hao-Hua HE, Xiao-Tang SUN, and Chang-Lan ZHU
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Plant Science ,Agronomy and Crop Science ,Biotechnology - Published
- 2022
13. Tuning the Solid- and Solution-State Fluorescence of the Iron-Chelator Deferasirox
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Xi-Le Hu, Adam C. Sedgwick, Daniel N. Mangel, Ying Shang, Axel Steinbrueck, Kai-Cheng Yan, Ling Zhu, Dylan W. Snelson, Sajal Sen, Calvin V. Chau, Gabriel Juarez, Vincent M. Lynch, Xiao-Peng He, and Jonathan L. Sessler
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Methicillin-Resistant Staphylococcus aureus ,Deferasirox ,Colloid and Surface Chemistry ,Pseudomonas aeruginosa ,Microbial Sensitivity Tests ,General Chemistry ,Iron Chelating Agents ,Biochemistry ,Fluorescence ,Catalysis ,Anti-Bacterial Agents - Abstract
Deferasirox, an FDA-approved iron chelator, has gained increasing attention for use in anticancer and antimicrobial applications. Recent efforts by our group led to the identification of this core as an easy-to-visualize aggregation-induced emission platform, or AIEgen, that provides a therapeutic effect equivalent to deferasirox (
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- 2022
14. Human serum albumin-based supramolecular host–guest boronate probe for enhanced peroxynitrite sensing
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He Tian Jr., Chen Guo, Xi-Le Hu, Jing-Bo Wang, Yi Zang, Tony D. James, Jia Li, and Xiao-Peng He
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Organic Chemistry ,Physical and Theoretical Chemistry ,Biochemistry - Abstract
Host–guest inclusion of a small-molecule fluorescent probe into human serum albumin enhances its sensitivity for ONOO− in aqueous solution and in cells.
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- 2023
15. Ratiometric sensing of β-galactosidase based on excited-state intramolecular proton transfer (ESIPT) and solid-state luminescence enhancement
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He Tian, Wei Lin, Xi-Le Hu, Jing-Bo Wang, Min-Yu Zhang, Yi Zang, Xin-Yan Wu, Jia Li, Tony D. James, and Xiao-Peng He
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Organic Chemistry - Abstract
An ESIPT-based ratiometric probe with solid-state luminescence enhancement is developed for the sensitive detection of glycosidase activity.
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- 2023
16. Molecularly engineered AIEgens with enhanced quantum and singlet-oxygen yield for mitochondria-targeted imaging and photodynamic therapy
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Fang-Zhou Xu, Ling Zhu, Hai-Hao Han, Jian-Wei Zou, Yi Zang, Jia Li, Tony D. James, Xiao-Peng He, and Cheng-Yun Wang
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General Chemistry - Abstract
Luminogens characteristic of aggregation-induced emission (AIEgens) have been extensively exploited for the development of imaging-guided photodynamic therapeutic (PDT) agents. However, intramolecular rotation of donor-acceptor (D-A) type AIEgens favors non-radiative decay of photonic energy which results in unsatisfactory fluorescence quantum and singlet oxygen yields. To address this issue, we developed several molecularly engineered AIEgens with partially "locked" molecular structures enhancing both fluorescence emission and the production of triplet excitons. A triphenylphosphine group was introduced to form a D-A conjugate, improving water solubility and the capacity for mitochondrial localization of the resulting probes. Experimental and theoretical analyses suggest that the much higher quantum and singlet oxygen yield of a structurally "significantly-locked" probe (LOCK-2) than its "partially locked" (LOCK-1) and "unlocked" equivalent (LOCK-0) is a result of suppressed AIE and twisted intramolecular charge transfer. LOCK-2 was also used for the mitochondrial-targeting, fluorescence image-guided PDT of liver cancer cells.
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- 2022
17. Targeted delivery of maytansine to liver cancer cells via galactose-modified supramolecular two-dimensional glycomaterial
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Hai-Na Xie, Yu-Yuan Chen, Guo-Biao Zhu, Hai-Hao Han, Xi-Le Hu, Zhi-Qiang Pan, Yi Zang, Dong-Hao Xie, Xiao-Peng He, Jia Li, and Tony D. James
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Materials Chemistry ,Metals and Alloys ,Ceramics and Composites ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Abstract
A two-dimensional (2D) glycomaterial for targeted delivery of maytansine to liver cancer cells was developed.
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- 2022
18. Iodine substitution compromises the aggregation-induced emission (AIE) property and enhances the biothiol sensitivity of quinoline–malononitrile-based fluorescent probes
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Guo-Biao Zhu, Jie Gao, Min-Yu Zhang, Liu He, Hai-Hao Han, Yi Zang, Jia Li, and Xiao-Peng He
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Process Chemistry and Technology ,General Chemical Engineering - Published
- 2023
19. Dual-Channel Fluorescent Probe for the Simultaneous Monitoring of Peroxynitrite and Adenosine-5′-triphosphate in Cellular Applications
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Luling Wu, Jihong Liu, Xue Tian, Robin R. Groleau, Beidou Feng, Yonggang Yang, Adam C. Sedgwick, Hai-Hao Han, Yang Wang, Han-Min Wang, Fang Huang, Steven D. Bull, Hua Zhang, Chusen Huang, Yi Zang, Jia Li, Xiao-Peng He, Ping Li, Bo Tang, Tony D. James, and Jonathan L. Sessler
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inorganic chemicals ,Colloid and Surface Chemistry ,Peroxynitrous Acid ,General Chemistry ,Biochemistry ,Article ,Catalysis - Abstract
Changes in adenosine triphosphate (ATP) and peroxynitrite (ONOO–) concentrations have been correlated in a number of diseases including ischemia-reperfusion injury and drug-induced liver injury. Herein, we report the development of a fluorescent probe ATP-LW, which enables the simultaneous detection of ONOO– and ATP. ONOO– selectively oxidizes the boronate pinacol ester of ATP-LW to afford the fluorescent 4-hydroxy-1,8-naphthalimide product NA-OH (λex = 450 nm, λem = 562 nm or λex = 488 nm, λem = 568 nm). In contrast, the binding of ATP to ATP-LW induces the spirolactam ring opening of rhodamine to afford a highly emissive product (λex = 520 nm, λem = 587 nm). Due to the differences in emission between the ONOO– and ATP products, ATP-LW allows ONOO– levels to be monitored in the green channel (λex = 488 nm, λem = 500–575 nm) and ATP concentrations in the red channel (λex = 514 nm, λem = 575–650 nm). The use of ATP-LW as a combined ONOO– and ATP probe was demonstrated using hepatocytes (HL-7702 cells) in cellular imaging experiments. Treatment of HL-7702 cells with oligomycin A (an inhibitor of ATP synthase) resulted in a reduction of signal intensity in the red channel and an increase in that of the green channel as expected for a reduction in ATP concentrations. Similar fluorescence changes were seen in the presence of SIN-1 (an exogenous ONOO– donor).
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- 2021
20. TCF-based fluorescent probe for monitoring superoxide anion produced in bacteria under chloramphenicol- and heat-induced stress
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Kai-Cheng Yan, Bethany L. Patenall, Jordan E. Gardiner, Rachel A. Heylen, Naing Thet, Xiao-Peng He, Adam C. Sedgwick, Tony D. James, and A. Toby A. Jenkins
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Bacteria ,Metals and Alloys ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Anti-Bacterial Agents ,Chloramphenicol ,Superoxides ,Pseudomonas aeruginosa ,Materials Chemistry ,Ceramics and Composites ,Escherichia coli ,Enterococcus faecalis ,Heat-Shock Response ,Fluorescent Dyes - Abstract
We report on a superoxide anion (O2˙−) responsive fluorescent probe called TCF-OTf.
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- 2022
21. Refractory chylothorax after esophagectomy caused by injured thoracic duct with a rare collateral branch: A case report
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Jianyu Wang, Xiangyu Chen, Liang Song, Xiang-Yan Liu, and Xiao-Peng He
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Background Chylothorax is a rare but serious complication of esophagectomy that can be life threatening if not addressed in a timely fashion. Chylous leakage generally occurs in response to thoracic duct injury, and anatomical variations in thoracic duct structure can add to the risk of injury during esophageal surgery. Here, we report a rare occurrence of refractory chylothorax after esophagectomy and discuss the diagnostic strategy and aggressive surgical management for this condition, along with a review of the literature. Case presentation We report a 62-year-old man with refractory chylothorax that occurred after esophagectomy caused by a hidden collateral branch of the injured thoracic duct with a rare positional relationship to the injury site. The chylous fluid from the unrevealed right-sided duct drained into the injured left-sided thoracic duct via the collateral branch opposite the injury on the side wall of the thoracic duct and continually leaked out through the injury. After ligation of the hidden branch, the patient was cured and discharged 2 wk later. Conclusion This is a rare case of distal partial duplication of the thoracic duct, and anatomical variation of the thoracic duct can still lead to chylothorax even if its main trunk has been ligated prophylactically. Care must be taken to adequately ligate both trunks in cases of distal duplication as there is shared proximal trunk, and hidden collateral branches should be carefully explored at the same time.
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- 2022
22. Graphene nanoribbon-based supramolecular ensembles with dual-receptor targeting function for targeted photothermal tumor therapy†
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Chen Xi Xu, Tony D. James, Hong Bo Ru, Adam C. Sedgwick, Jie Gao, Xiangfeng Luan, He Tian, Xiao-Peng He, Guo Rong Chen, Jiacheng Zhang, Yiyong Mai, Ling Zhu, Jia Li, Yi Zang, Wei Tao Dou, and Fugui Xu
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Chemistry ,Graphene ,02 engineering and technology ,General Chemistry ,Photothermal therapy ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Ligand (biochemistry) ,medicine.disease ,01 natural sciences ,In vitro ,0104 chemical sciences ,law.invention ,Breast cancer ,In vivo ,law ,Cancer research ,medicine ,0210 nano-technology ,Receptor ,Triple-negative breast cancer - Abstract
Triple negative breast cancer (TNBC) is one of the most malignant subtypes of breast cancer. Here, we report the construction of graphene nanoribbon (GNR)-based supramolecular ensembles with dual-receptor (mannose and αvβ3 integrin receptors) targeting function, denoted as GNR-Man/PRGD, for targeted photothermal treatment (PTT) of TNBC. The GNR-Man/PRGD ensembles were constructed through the solution-based self-assembly of mannose-grafted GNRs (GNR-Man) with a pyrene-tagged αvβ3 integrin ligand (PRGD). Enhanced PTT efficacies were achieved both in vitro and in vivo compared to that of the non-targeting equivalents. Tumor-bearing live mice were administered (tail vein) with GNR-Man/PRGD and then each mice group was subjected to PTT. Remarkably, GNR-Man/PRGD induced complete ablation of the solid tumors, and no tumor regrowth was observed over a period of 15 days. This study demonstrates a new and promising platform for the development of photothermal nanomaterials for targeted tumor therapy., Dual receptor-targeting supramolecular glycomaterials are constructed based on graphene nanoribbons for the targeted photothermal therapy of triple-negative breast cancer in vivo.
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- 2021
23. Metabolically Specific
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Chen-Jian, Zhong, Xi-Le, Hu, Xiao-Lan, Yang, Hui-Qi, Gan, Kai-Cheng, Yan, Fu-Ting, Shu, Pei, Wei, Teng, Gong, Peng-Fei, Luo, Tony D, James, Zhao-Hong, Chen, Yong-Jun, Zheng, Xiao-Peng, He, and Zhao-Fan, Xia
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Mammals ,Dihydropteroate Synthase ,Mice ,Folic Acid ,Animals ,Humans ,Bacterial Infections ,4-Aminobenzoic Acid - Abstract
The ability to effectively detect bacterial infection in human tissues is important for the timely treatment of the infection. However, traditional techniques fail to visualize bacterial species adhered to host cells
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- 2022
24. Fluorescent probes for the detection of disease-associated biomarkers
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Wei-Tao Dou, Hai-Hao Han, Adam C. Sedgwick, Guo-Biao Zhu, Yi Zang, Xin-Rong Yang, Juyoung Yoon, Tony D. James, Jia Li, and Xiao-Peng He
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Multidisciplinary ,Fluorescent probes ,Biomarker detection ,General ,Chemical biology ,Biomarkers ,Fluorescence imaging ,Fluorescent Dyes - Abstract
Fluorescent probes have emerged as indispensable chemical tools to the field of chemical biology and medicine. The ability to detect intracellular species and monitor physiological processes has not only advanced our knowledge in biology but has provided new approaches towards disease diagnosis. In this review, we detail the design criteria and strategies for some recently reported fluorescent probes that can detect a wide range of biologically important species in cells and in vivo. In doing so, we highlight the importance of each biological species and their role in biological systems and for disease progression. We then discuss the current problems and challenges of existing technologies and provide our perspective on the future directions of the research area. Overall, we hope this review will provide inspiration for researchers and prove as useful guide for the development of the next generation of fluorescent probes.
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- 2022
25. Targeted delivery of maytansine to liver cancer cells
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Hai-Na, Xie, Yu-Yuan, Chen, Guo-Biao, Zhu, Hai-Hao, Han, Xi-Le, Hu, Zhi-Qiang, Pan, Yi, Zang, Dong-Hao, Xie, Xiao-Peng, He, Jia, Li, and Tony D, James
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Cell Line, Tumor ,Liver Neoplasms ,Galactose ,Humans ,Maytansine ,Serum Albumin, Human ,Cell Line - Abstract
A two-dimensional (2D) glycomaterial for targeted delivery of maytansine to liver cancer cells was developed. Host-guest interaction between a galactosyl dye and human serum albumin (HSA) produces supramolecular galactoside-HSA conjugates, which are then used to coat 2D MoS
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- 2022
26. The Evaluation of Ester Functionalised TCF‐Based Fluorescent Probes for the Detection of Bacterial Species
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Kira L. F. Hilton, Lauren Gwynne, Tony D. James, Xiao-Peng He, Bethany L. Patenall, A. Toby A. Jenkins, George T. Williams, Jean-Yves Maillard, Kai-Cheng Yan, Adam C. Sedgwick, Jennifer R. Hiscock, and Jordan E. Gardiner
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chemistry.chemical_classification ,Detection limit ,biology ,010405 organic chemistry ,Chemistry ,Pseudomonas aeruginosa ,General Chemistry ,010402 general chemistry ,medicine.disease_cause ,biology.organism_classification ,01 natural sciences ,Esterase ,Fluorescence ,0104 chemical sciences ,Amino acid ,Biochemistry ,Staphylococcus aureus ,QD431 ,medicine ,Escherichia coli ,Bacteria - Abstract
The ester functionality is commonly seen in the areas of chemical biology and medicinal chemistry for the design of cell‐permeable active molecules. Ester‐based pro‐drug/pro‐sensor strategies are employed to mask polar functional groups (i. e. carboxylic acids) and improve the overall cell permeability of these functional molecules. However, their use as reactive units for sensing applications, including bacterial detection, has not been fully explored. Herein, we synthesised two TCF‐based fluorescent probes, TCF‐OAc and TCF‐OBu. As expected, both TCF‐OAc and TCF‐OBu demonstrated a significant fluorescence (22‐ and 43‐fold, respectively) and colorimetric response (yellow to purple) towards porcine liver esterase (PLE) with a limit of detection of 1.18 mU/mL and 0.45 mU/mL, respectively. With these results in hand, the ability of these probes to detect planktonic suspensions of gram‐positive Staphylococcus aureus (S. aureus) and gram‐negative Pseudomonas aeruginosa (P. aeruginosa), and Escherichia coli (E. coli) were evaluated. Different fluorescence responses for gram‐positive and gram‐negative bacteria were observed between TCF‐OAc and TCF‐OBu. After 1 h incubation, TCF‐OAc proved more sensitive towards S. aureus, demonstrating a significant fluorescence “turn on” response (16‐fold); whereas, TCF‐OBu was more selective towards P. aeruginosa, with a 22‐fold increase in the fluorescence response observed. These results demonstrate the influence of the ester chain length on the selectivity for bacterial species.
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- 2021
27. Stimulus-responsive water soluble synthetic nanographene
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Yanpeng Zhu, Shuang Ma, Xiao-Peng He, Wei-Tao Dou, Jiaobing Wang, and Ying Chen
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Work (thermodynamics) ,Aqueous solution ,Materials science ,Photothermal effect ,02 engineering and technology ,General Chemistry ,Atmospheric temperature range ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Lower critical solution temperature ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Thermal ,Irradiation ,0210 nano-technology ,Triethylene glycol - Abstract
Nanographenes (NGs) are exceptionally hydrophobic. They are insoluble in water, preventing the exploration and utilization of their photophysical properties under aqueous conditions. This work discloses an atomically precise water-soluble synthetic NG 1, featuring a 2 nm sp2 carbon skeleton appended with 12 branched triethylene glycol chains. It synergistically combines low critical solution temperature (LCST) behavior and a photothermal effect to create the first thermo- and photo-responsive atomically precise NG functioning in an aqueous solution. The LCST behavior can be attributed to a delicate balance of hydrophobic-hydrophilic interactions, providing a sensitive thermal response to changes over a temperature range of physiological interest (close to 37 °C). Moreover, 1 has considerable photothermal conversion capability, with irradiation of 1 in water by red or near infrared light increasing the solutions temperature to above the clouding point within seconds, leading to a reversible clear-to-turbid transition over many cycles without evident fatigue.
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- 2021
28. In vitro studies of deferasirox derivatives as potential organelle-targeting traceable anti-cancer therapeutics
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Dan-Ying Huang, Hai-Hao Han, Axel Steinbrueck, Xiao-Peng He, Adam C. Sedgwick, Michael Y. Zhao, Sajal Sen, Jia Li, Yi Zang, and Jonathan L. Sessler
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Article ,Catalysis ,03 medical and health sciences ,0302 clinical medicine ,Fluorescent cell ,Lysosome ,Organelle ,Materials Chemistry ,medicine ,Lung cancer ,030304 developmental biology ,0303 health sciences ,Chemistry ,Deferasirox ,Metals and Alloys ,Cancer ,General Chemistry ,medicine.disease ,In vitro ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Ceramics and Composites ,Cancer research ,medicine.drug - Abstract
We report here strategic functionalization of the FDA approved chelator deferasirox (1) in an effort to produce organelle-targeting iron chelators with enhanced activity against A549 lung cancer cells. Derivative 8 was found to have improved antiproliferative activity relative to 1. Fluorescent cell imaging revealed that compound 8 preferentially localises within the lysosome.
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- 2021
29. TCF-ALP: a fluorescent probe for the selective detection of Staphylococcus bacteria and application in 'smart' wound dressings
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Tony D. James, Kai Cheng Yan, A. Toby A. Jenkins, Xiao-Peng He, Jean-Yves Maillard, George T. Williams, Jordan E. Gardiner, Lauren Gwynne, Bethany L. Patenall, and Adam C. Sedgwick
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biology ,Pseudomonas aeruginosa ,Chemistry ,Biomedical Engineering ,Biofilm ,Pathogenic bacteria ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,medicine.disease_cause ,biology.organism_classification ,01 natural sciences ,Enterococcus faecalis ,0104 chemical sciences ,Microbiology ,stomatognathic system ,Staphylococcus aureus ,medicine ,General Materials Science ,0210 nano-technology ,Escherichia coli ,Bacteria ,Ex vivo - Abstract
Alkaline phosphatase (ALP) is an important enzyme-based biomarker present in several bacterial species; however, it is currently undervalued as a strategy to detect pathogenic bacteria. Here, we explore our ALP-responsive colorimetric and fluorescent probe (TCF-ALP) for such applications. TCF-ALP displayed a colorimetric and fluorescence response towards Staphylococcus aureus (S. aureus), with a limit of detection of 3.7 × 106 CFU mL−1 after 24 h incubation. To our surprise, TCF-ALP proved selective towards Staphylococcus bacteria when compared with Enterococcus faecalis (E. faecalis), and Gram-negative P. aeruginosa and E. coli. Selectivity was also seen in clinically relevant S. aureus biofilms. Owing to the high prevalence and surface location of S. aureus in chronic wounds, TCF-ALP was subsequently encapsulated in polyvinyl alcohol (PVA)-based hydrogels as a proof-of-concept “smart” wound dressing. TCF-ALP hydrogels were capable of detecting S. aureus in planktonic and biofilm assays, and displayed a clear colour change from yellow to purple after 24 h incubation using ex vivo porcine skin models. Overall, TCF-ALP is a simple tool that requires no prior knowledge, training, or specialist equipment, and has the potential to overcome issues related to invasive swabbing and tissue biopsy methods. Thus, TCF-ALP could be used as a tool to monitor the early development of infection in a wound and allow for the rapid provision of appropriate treatment for Staphylococcal bacterial infections.
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- 2021
30. Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers
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Hai Hao Han, Yao Li, Tony D. James, Xi Le Hu, Yi Zang, Na Li, Xianzhi Chai, Yan Wang, Adam C. Sedgwick, Junji Zhang, Xiao-Peng He, He Tian, Jia Li, and Yang Yu
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Chemistry(all) ,Photoisomerization ,Serum Albumin, Human ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Catalysis ,Cell Line ,Photochromism ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,SDG 3 - Good Health and Well-being ,Microscopy ,medicine ,Humans ,Merocyanine ,Fluorescent Dyes ,Spiropyran ,Microscopy, Confocal ,Molecular Structure ,Optical Imaging ,General Chemistry ,Photochemical Processes ,beta-Galactosidase ,Human serum albumin ,Fluorescence ,0104 chemical sciences ,body regions ,chemistry ,embryonic structures ,Biophysics ,Phototoxicity ,Biomarkers ,medicine.drug - Abstract
Here, we report a β-galactosidase (β-Gal)-responsive photochromic fluorescent probe, NpG, that was designed to prebind to human serum albumin (HSA) to form the probe/protein hybrid, NpG@HSA. The formation of NpG@HSA led to an increase in fluorescence emission (520 nm) corresponding to the binding of the fluorescent naphthalimide unit with HSA. In addition, this enabled visualization of the spiropyran fluorescence emission in aqueous media. Our probe/protein hybrid approach afforded a unique imaging platform with enhanced cell permeability and solubility that was capable of visualizing the cellular uptake of NpG@HSA before its activation by β-Gal. The β-Gal-mediated cleavage of the galactose unit within the NpG@HSA hybrid resulted in the formation of NpM@HSA and an increase in red fluorescence emission (620 nm). The resultant merocyanine unit was then able to undergo photoisomerization (merocyanine ↔ spiropyran) to facilitate STORM (i.e., stochastic optical reconstruction microscopy) imaging with minimal phototoxicity and excellent photostability/reversibility. Using STORM, NpG@HSA was able to determine the subcellular distribution of β-Gal activity between cell lines with nanoscale precision. We believe that this system represents a versatile imaging platform for the design of photochromic fluorescent probes suitable for illuminating the precise location of disease-specific biomarkers in various cellular processes.
- Published
- 2020
31. Pinkment: a synthetic platform for the development of fluorescent probes for diagnostic and theranostic applications†
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Maria Weber, Amanda B. Mackenzie, Tony D. James, Jia Li, Maria L. Odyniec, Xiao-Peng He, Yi Zang, Bo Han Li, Charlotte E.F. Jarman, Adam C. Sedgwick, Hai Hao Han, and Steven D. Bull
- Subjects
Chemistry ,Bioconjugation ,Biological species ,Nanotechnology ,General Chemistry ,Fluorescence ,Biomarker (cell) - Abstract
Reaction-based fluorescent-probes have proven successful for the visualisation of biological species in various cellular processes. Unfortunately, in order to tailor the design of a fluorescent probe to a specific application (i.e. organelle targeting, material and theranostic applications) often requires extensive synthetic efforts and the synthetic screening of a range of fluorophores to match the required synthetic needs. In this work, we have identified Pinkment-OH as a unique “plug-and-play” synthetic platform that can be used to develop a range of ONOO− responsive fluorescent probes for a variety of applications. These include theranostic-based applications and potential material-based/bioconjugation applications. The as prepared probes displayed an excellent sensitivity and selectivity for ONOO− over other ROS. In vitro studies using HeLa cells and RAW 264.7 macrophages demonstrated their ability to detect exogenously and endogenously produced ONOO−. Evaluation in an LPS-induced inflammation mouse model illustrated the ability to monitor ONOO− production in acute inflammation. Lastly, theranostic-based probes enabled the simultaneous evaluation of indomethacin-based therapeutic effects combined with the visualisation of an inflammation biomarker in RAW 264.7 cells., Pinkment, a resorufin based ONOO− selective and sensitive ‘plug and play’ fluorescence-based platform for in vitro and in vivo use, enables facile functionalisation for various imaging and theranostic applications.
- Published
- 2020
32. Emission Characteristics of All-Silicon Distributed Feedback Lasers With a Wide Gain Range
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Dong-Chen Wang, Pan Zeng, Xiao-Peng He, Wen-Jie Zhou, Chi Zhang, Shuyu Zhang, Haotian Wang, Qing-Yuan Jin, Yuchen Zhang, Ming Lu, and Xiang Wu
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Materials science ,Photoluminescence ,Silicon ,business.industry ,chemistry.chemical_element ,Laser ,Atomic and Molecular Physics, and Optics ,law.invention ,Optical pumping ,chemistry ,law ,Femtosecond ,Optoelectronics ,Electrical and Electronic Engineering ,Photonics ,business ,Lasing threshold ,Tunable laser - Abstract
With the development of nanomanufacturing methods, the manipulation of photons down to the nanoscale in silicon integrated optical chips has become a feasible and promising solution for next-generation data processing as electronic chips reach their limit. As an essential active device that generates photons for all other working photonic components, silicon lasers are the last barrier to achieve silicon integrated optical chips. Although optical gain in silicon nanocrystals (Si–NCs) was observed in 2000, the progress in realizing all-Si lasers has been very limited due to the inferior optical gain compared to traditional gain materials. In this paper, highly luminescent thin films of Si–NCs with a photoluminescence quantum yield of 57% are developed. The broadband photoluminescence covers the wavelength range from 650 to 900 nm, and wide-range optical gains are identified, indicating the feasibility of a tunable laser. Distributed feedback (DFB) all-Si lasers are fabricated using these thin films and pumped by femtosecond pulses. Various characteristic lasing behaviors are observed. Additionally, three different DFB grating periods are selected, and the lasing peak can be tuned by over 100 nm. The lasing thresholds range from 8.3 to 53.3 MW/cm2. The linewidths of lasing peaks are less than 2 nm.
- Published
- 2020
33. EFEMP2 Suppresses the Invasion of Lung Cancer Cells by Inhibiting Epithelial-Mesenchymal Transition (EMT) and Down-Regulating MMPs
- Author
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Liang Song, Bin Jiang, Zhou Wang, Xiao-Peng He, Xiangyan Liu, Mo Shi, Yang Yu, and Xiang-Xin Li
- Subjects
0301 basic medicine ,Gene knockdown ,MMP2 ,Tumor suppressor gene ,business.industry ,Cancer ,respiratory system ,MMP9 ,medicine.disease ,respiratory tract diseases ,Metastasis ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Medicine ,Pharmacology (medical) ,Epithelial–mesenchymal transition ,business ,Lung cancer - Abstract
Background Epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (EFEMP2), also known as fibulin-4, MBP1 and UPH1, is an extracellular matrix protein associated with a variety of tumors. The purpose of this study was to investigate the prognostic value and the function of EFEMP2 in lung cancer. Methods The mRNA and protein expression of EFEMP2 in lung normal and cancer tissues, lung cancer cell lines (A549, H460, H1299 and H1650) and normal epithelial cell line BEAS-2B were evaluated by immunohistochemistry, RT-qPCR and Western blotting. The Public databases (Oncomine and Kaplan-Meier plotter) were used to investigate the prognostic value of EFEMP2 in lung cancer. RNA interference (RNAi) and overexpression transfection were performed to detect the effects of EFEMP2 up- or down-regulation on lung normal and cancer cell proliferation, invasion and metastasis in vitro and in vivo. Results EFEMP2 was lowly expressed in lung cancer tissues and cells, and its low expression was associated with malignant phenotype and poor prognosis of lung cancer. The same conclusion had been drawn from the Public databases. EFEMP2 overexpression significantly inhibited the invasion of lung cancer cells, hampered the process of EMT, and decreased the expression and activity of MMP2 and MMP9, while EFEMP2 knockdown remarkably enhanced the invasion of lung cancer cells, promoted EMT, and increased the expression and activity of MMP2 and MMP9. Conclusion The low expression of EFEMP2 was detected in lung cancer and was positively correlated with the poor prognosis of patients. EFEMP2 was a tumor suppressor gene that inhibited the progress of lung cancer, which suggested a new research objective for the future studies.
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- 2020
34. A Supramolecular‐Based Dual‐Wavelength Phototherapeutic Agent with Broad‐Spectrum Antimicrobial Activity Against Drug‐Resistant Bacteria
- Author
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Fugui Xu, Nahyun Kwon, Xingshu Li, Zhi Hao Yu, He Tian, Yiyong Mai, Xiaojing Dong, Juyoung Yoon, Xi Le Hu, Jiatao Yan, Tingting Tang, Guo Rong Chen, Daijie Chen, and Xiao-Peng He
- Subjects
Methicillin-Resistant Staphylococcus aureus ,Porphyrins ,Light ,medicine.medical_treatment ,Nanoparticle ,Nanotechnology ,Photodynamic therapy ,010402 general chemistry ,01 natural sciences ,Catalysis ,Nanocomposites ,Polyethylene Glycols ,chemistry.chemical_compound ,Antibiotic resistance ,Anti-Infective Agents ,Drug Resistance, Bacterial ,Gram-Negative Bacteria ,medicine ,Nanotubes ,Nanocomposite ,010405 organic chemistry ,Chemistry ,General Medicine ,General Chemistry ,Photothermal therapy ,Antimicrobial ,Porphyrin ,0104 chemical sciences ,Graphite ,Reactive Oxygen Species ,Graphene nanoribbons - Abstract
With the ever-increasing threat posed by the multi-drug resistance of bacteria, the development of non-antibiotic agents for the broad-spectrum eradication of clinically prevalent superbugs remains a global challenge. Here, we demonstrate the simple supramolecular self-assembly of structurally defined graphene nanoribbons (GNRs) with a cationic porphyrin (Pp4N) to afford unique one-dimensional wire-like GNR superstructures coated with Pp4N nanoparticles. This Pp4N/GNR nanocomposite displays excellent dual-modal properties with significant reactive-oxygen-species (ROS) production (in photodynamic therapy) and temperature elevation (in photothermal therapy) upon light irradiation at 660 and 808 nm, respectively. This combined approach proved synergistic, providing an impressive antimicrobial effect that led to the complete annihilation of a wide spectrum of Gram-positive, Gram-negative, and drug-resistant bacteria both in vitro and in vivo. The study also unveils the promise of GNRs as a new platform to develop dual-modal antimicrobial agents that are able to overcome antibiotic resistance.
- Published
- 2020
35. Supramolecular fluorogenic peptide sensor array based on graphene oxide for the differential sensing of ebola virus
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Dong Ming Zhou, Guo Rong Chen, Tony D. James, Xiao-Peng He, Meng Qi Fu, Wei Tao Dou, and Xu Chen Wang
- Subjects
viruses ,Supramolecular chemistry ,Peptide ,Biosensing Techniques ,medicine.disease_cause ,Catalysis ,law.invention ,Marburg virus ,Sensor array ,law ,Materials Chemistry ,medicine ,Fluorescent Dyes ,Glycoproteins ,chemistry.chemical_classification ,Ebola virus ,biology ,Graphene ,Metals and Alloys ,Vesiculovirus ,General Chemistry ,Ebolavirus ,biology.organism_classification ,Virology ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Marburgvirus ,chemistry ,Vesicular stomatitis virus ,Ceramics and Composites ,Capsid Proteins ,Graphite ,Peptides ,Glycoprotein - Abstract
We report on a supramolecular sensor array using fluorogenic peptide probes and graphene oxide that can target glycoproteins on a viral caspid, facilitating the differentiation of ebola virus from marburg virus and receptor-extensive vesicular stomatitis virus using principal component analysis.
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- 2020
36. A glycoconjugate-based gold nanoparticle approach for the targeted treatment of Pseudomonas aeruginosa biofilms
- Author
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Xi Le Hu, Tony D. James, Guo Rong Chen, Chao Zhang, Daijie Chen, Xiao-Peng He, Bing Ye, De Tai Shi, Adam C. Sedgwick, and Kai Cheng Yan
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chemistry.chemical_classification ,Fluorescence-lifetime imaging microscopy ,Fluorophore ,Glycoconjugate ,Pseudomonas aeruginosa ,Biofilm ,Photothermal therapy ,medicine.disease_cause ,chemistry.chemical_compound ,chemistry ,Targeted drug delivery ,Colloidal gold ,medicine ,Biophysics ,General Materials Science - Abstract
In this study, "core-shell" gold nanoparticles (AuNPs) have been functionalised using a simple one-pot approach to form fucose-based glycoconjugate AuNPs (Fuc-AuNPs) and galactose-based glycoconjugate AuNPs (Gal-AuNPs), respectively. Owing to the selective carbohydrate-based recognition of the key virulence factors of P. aeruginosa, LecB (fucose-specific lectin)/LecA (galactose-specific lectin), Fuc-AuNPs and Gal-AuNPs-based imaging and therapeutic strategies were evaluated towards P. aeruginosa. Both Fuc-AuNPs and Gal-AuNPs were non-covalently loaded with the fluorophore dicyanomethylene 4H-pyran (DCM) to afford two highly selective fluorescence imaging agents for the visualisation of P. aeruginosa. The loading of Fuc-AuNPs and Gal-AuNPs with the known antibiotic Ceftazidime (CAZ) exhibited an enhanced therapeutic effect, illustrating the significance of this targeted drug delivery strategy. Exploiting the phototherapeutic properties of AuNPs, photoirradiation (600 nm) of Fuc-AuNP@CAZ/Gal-AuNP@CAZ provided both photothermal and photodynamic therapeutic (PTT/PDT) effects, which facilitated the release of CAZ. Fuc-AuNP@CAZ and Gal-AuNP@CAZ were shown to be effective photo/chemotherapeutics resulting in almost complete eradication of P. aeruginosa biofilms formed on clinically relevant surfaces (glass slides and steel surface).
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- 2020
37. Metal-based imaging agents: progress towards interrogating neurodegenerative disease
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James T. Brewster, Tony D. James, Adam C. Sedgwick, Jonathan L. Sessler, Graham Smith, Xiao-Peng He, Diana A. Iovan, Peter Harvey, and He Tian
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Tomography, Emission-Computed, Single-Photon ,medicine.diagnostic_test ,business.industry ,Disease progression ,Neurodegeneration ,Neurodegenerative Diseases ,Magnetic resonance imaging ,General Chemistry ,Disease ,medicine.disease ,Magnetic Resonance Imaging ,Biomarker (cell) ,Coordination Complexes ,Metals ,Positron emission tomography ,Positron-Emission Tomography ,Transition Elements ,medicine ,Animals ,Humans ,Indicators and Reagents ,Molecular imaging ,business ,Neuroscience ,Emission computed tomography - Abstract
Central nervous system (CNS) neurodegeneration is defined by a complex series of pathological processes that ultimately lead to death. The precise etiology of these disorders remains unknown. Recent efforts show that a mechanistic understanding of the malfunctions underpinning disease progression will prove requisite in developing new treatments and cures. Transition metals and lanthanide ions display unique characteristics (i.e., magnetism, radioactivity, and luminescence), often with biological relevance, allowing for direct application in CNS focused imaging modalities. These techniques include positron emission tomography (PET), single-photon emission computed tomography (SPECT), magnetic resonance imaging (MRI), and luminescent-based imaging (LumI). In this Tutorial Review, we have aimed to highlight the various metal-based imaging techniques developed in the effort to understand the pathophysiological processes associated with neurodegeneration. Each section has been divided so as to include an introduction to the particular imaging technique in question. This is then followed by a summary of key demonstrations that have enabled visualization of a specific neuropathological biomarker. These strategies have either exploited the high binding affinity of a receptor for its corresponding biomarker or a specific molecular transformation caused by a target species, all of which produce a concomitant change in diagnostic signal. Advantages and disadvantages of each method with perspectives on the utility of molecular imaging agents for understanding the complexities of neurodegenerative disease are discussed.
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- 2020
38. Transition metal chelators, pro-chelators, and ionophores as small molecule cancer chemotherapeutic agents
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Daniel M. Knoll, Ying Shang, Gabriela I. Vargas-Zúñiga, Axel Steinbrueck, Adam C. Sedgwick, James T. Brewster, Kai-Cheng Yan, Xiao-Peng He, Jonathan L. Sessler, and He Tian
- Subjects
Dose limiting toxicity ,Ionophores ,010405 organic chemistry ,business.industry ,Cancer ,Antineoplastic Agents ,General Chemistry ,Drug resistance ,010402 general chemistry ,medicine.disease ,01 natural sciences ,Small molecule ,0104 chemical sciences ,Coordination Complexes ,Cancer stem cell ,Neoplasms ,Cancer cell ,Transition Elements ,Cancer research ,Humans ,Medicine ,Treatment resistance ,business ,Cytotoxicity ,Chelating Agents - Abstract
Cancer is among the leading causes of death worldwide. Although a number of new treatment options have been developed in recent years, there remains a need for improved chemotherapies. The primary challenges facing new cancer drugs include: (1) improving patient quality of life, (2) overcoming drug resistance and (3) lowering reoccurrence rates. Major drawbacks of current chemotherapeutics arise from poor selectivity towards cancer cells, dose limiting toxicities, compliance-reducing side effects, and an inability to address resistance mechanisms. Chemotherapeutics that fail to achieve complete eradication of the disease can also lead to relapse and promote treatment resistance. New strategies to overcome these drawbacks include the use of transition metal chelators and ionophores to alter selectively the concentrations of iron, copper, and zinc in cancer cells. A number of metal chelators have successfully demonstrated cytotoxicity and targeted activity against drug-resistant cancer cells; several have proved effective against cancer stem cells, a significant cause of tumour reoccurrence. However, problems with formulation and targeting have been noted. Recent efforts have thus focused on the design of pro-chelators, inactive versions of chelators that are designed to be activated in the tumour. This is an appealing strategy that may potentially increase efficacy towards cancer-resistant malignant cells. This Tutorial Review summarizes recent progress involving transition metal chelators, pro-chelators, and ionophores as potential cancer chemotherapeutics. We will focus on the reported agents that are able to coordinate iron, copper, and zinc.
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- 2020
39. Cyclodextrin-Based Peptide Self-Assemblies (Spds) That Enhance Peptide-Based Fluorescence Imaging and Antimicrobial Efficacy
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Xiao-Peng He, Xi-Le Hu, Jin-Biao Jiao, Stéphane Maisonneuve, Jia Li, Adam C. Sedgwick, Jonathan L. Sessler, He Tian, Yi Zang, Guanzhen Wang, and Juan Xie
- Subjects
Fluorescence-lifetime imaging microscopy ,Peptide ,Microbial Sensitivity Tests ,Gram-Positive Bacteria ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Article ,Catalysis ,Colloid and Surface Chemistry ,Gram-Negative Bacteria ,mental disorders ,Apoptosis Biomarker ,chemistry.chemical_classification ,Cyclodextrins ,Cyclodextrin ,Chemistry ,Antimicrobial efficacy ,Optical Imaging ,General Chemistry ,Antimicrobial ,Fluorescence ,Anti-Bacterial Agents ,0104 chemical sciences ,Peptides ,Intracellular - Abstract
As a result of their high specificity for their corresponding biological targets, peptides have shown significant potential in a range of diagnostic and therapeutic applications. However, their widespread use has been limited by their minimal cell permeability and stability in biological milieus. We describe here a hepta-dicyanomethylene-4H-pyran appended β-cyclodextrin (DCM(7)-β-CD) that acts as a delivery enhancing “host” for 1-bromonaphthalene-modified peptides, as demonstrated with peptide probes P1–P4. Interaction between the fluorescent peptides P1–P3 and DCM(7)-β-CD results in the hierarchical formation of unique supramolecular architectures, which we term supramolecular-peptide-dots (Spds). Each Spd (Spd-1, Spd-2, and Spd-3) was found to facilitate the intracellular delivery of the constituent fluorescent probes (P1–P3), thus allowing spatiotemporal imaging of an apoptosis biomarker (caspase-3) and mitosis. Spd-4, incorporating the antimicrobial peptide P4, was found to provide an enhanced therapeutic benefit against both Gram-positive and Gram-negative bacteria relative to P4 alone. In addition, a fluorescent Spd-4 was prepared, which revealed greater bacterial cellular uptake compared to the peptide alone (P4-FITC) in E. coli. (ATCC 25922) and S. aureus (ATCC 25923). This latter observation supports the suggestion that the Spd platform reported here has the ability to facilitate the delivery of a therapeutic peptide and provides an easy-to-implement strategy for enhancing the antimicrobial efficacy of known therapeutic peptides. The present findings thus serve to highlight a new and effective supramolecular delivery approach that is potentially generalizable to overcome limitations associated with functional peptides.
- Published
- 2019
40. Self-assembled sialyllactosyl probes with aggregation-enhanced properties for ratiometric detection and blocking of influenza viruses
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Wei-Tao Dou, Xiao-Peng He, Jun Li, Dongming Zhou, and Zhao-Yang Qin
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Multidisciplinary ,Förster resonance energy transfer ,Chemistry ,Blocking (radio) ,Biophysics ,010502 geochemistry & geophysics ,01 natural sciences ,Fluorescence ,0105 earth and related environmental sciences ,Red fluorescence ,Self assembled - Abstract
Infection and dissemination of influenza viruses (IVs) causes serious health concerns worldwide. However, effective tools for the accurate detection and blocking of IVs remain elusive. Here, we develop a new sialyllactosyl probe with self-assembled core-shell structure for the ratiometric detection and blocking of IVs. N,N′-diaryl-dihydrodibenzo[a,c]phenazines were used to form the core structure by hydrophobic assembly in an aqueous solution with an aggregation-enhanced blue fluorescence mission. Subsequently, dicyanomethylene-4H-pyran-based sialyllactosides were used for self-assembly with the core structure, producing the sialyllactosyl probe that emits a red fluorescence due to Forster resonance energy transfer. The probe developed has been proven to be available for (1) the fluorescence ratiometric detection of IVs through selective interaction with the sialyllactosyl-binding proteins on the virus surface, and (2) effectively blocking the invasion of human-infecting IVs towards host cells as accentuated by the sialyllactosides on the surface of the probes.
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- 2019
41. Targeted photoswitchable imaging of intracellular glutathione by a photochromic glycosheet sensor
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Xiao-Peng He, Yi Zang, Junji Zhang, Jia Li, Xianzhi Chai, He Tian, and Hai-Hao Han
- Subjects
Molecular switch ,photochromic glycosheet ,Organic Chemistry ,photoswitchable imaging ,Glutathione ,Fluorescence ,Full Research Paper ,lcsh:QD241-441 ,Chemistry ,2D MnO2 nanosheets ,chemistry.chemical_compound ,Photochromism ,Membrane ,lcsh:Organic chemistry ,chemistry ,intracellular GSH ,Biophysics ,lcsh:Q ,Asialoglycoprotein receptor ,lcsh:Science ,molecular switches ,Biosensor ,Intracellular - Abstract
The development of photochromic fluorescence sensors with dynamic and multiple-signaling is beneficial to the improvement of biosensing/imaging precision. However, elaborate designs with complicated molecular structures are always required to integrate these functions into one molecule. By taking advantages of both redox-active/high loading features of two-dimensional (2D) manganese dioxide (MnO2) and dynamic fluorescence photoswitching of photochromic sensors, we here design a hybrid photochromic MnO2 glycosheet (Glyco-DTE@MnO2) to achieve the photoswitchable imaging of intracellular glutathione (GSH). The photochromic glycosheet manifests significantly turn-on fluorescence and dynamic ON/OFF fluorescence signals in response to GSH, which makes it favorable for intracellular GSH double-check in targeted human hepatoma cell line (HepG2) through the recognition between β-D-galactoside and asialoglycoprotein receptor (ASGPr) on cell membranes. The dynamic fluorescence signals and excellent selectivity for detection and imaging of GSH ensure the precise determination of cell states, promoting its potential applications in future disease diagnosis and therapy.
- Published
- 2019
42. Ferrocene-Labelled Electroactive Aptamer-Based Sensors (Aptasensors) for Glycated Haemoglobin
- Author
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Yi Ju, Xiao-Peng He, Bang-Ce Ye, Xue-Qing Feng, Zhonggan Jin, Tony D. James, Wei-Tao Dou, and Qing Li
- Subjects
Analyte ,Metallocenes ,Aptamer ,Pharmaceutical Science ,electrochemical sensor ,Biosensing Techniques ,Electrochemistry ,Article ,Analytical Chemistry ,chemistry.chemical_compound ,QD241-441 ,Drug Discovery ,glycated haemoglobin ,Humans ,Ferrous Compounds ,Sulfhydryl Compounds ,Physical and Theoretical Chemistry ,Electrodes ,aptamer ,Glycated Hemoglobin ,Organic Chemistry ,Electrochemical Techniques ,Aptamers, Nucleotide ,Combinatorial chemistry ,Electrochemical gas sensor ,Dielectric spectroscopy ,Ferrocene ,chemistry ,Diabetes Mellitus, Type 2 ,Chemistry (miscellaneous) ,Dielectric Spectroscopy ,Electrode ,Molecular Medicine ,Differential pulse voltammetry ,Gold ,Biomarkers ,Protein Binding - Abstract
Glycated haemoglobin (HbA1c) is a diagnostic biomarker for type 2 diabetes. Traditional analytical methods for haemoglobin (Hb) detection rely on chromatography, which requires significant instrumentation and is labour-intensive; consequently, miniaturized devices that can rapidly sense HbA1c are urgently required. With this research, we report on an aptamer-based sensor (aptasensor) for the rapid and selective electrochemical detection of HbA1c. Aptamers that specifically bind HbA1c and Hb were modified with a sulfhydryl and ferrocene group at the 3′ and 5′-end, respectively. The modified aptamers were coated through sulfhydryl-gold self-assembly onto screen printed electrodes, producing aptasensors with built in electroactivity. When haemoglobin was added to the electrodes, the current intensity of the ferrocene in the sensor system was reduced in a concentration-dependent manner as determined by differential pulse voltammetry. In addition, electrochemical impedance spectroscopy confirmed selective binding of the analytes to the aptamer-coated electrode. This research offers new insight into the development of portable electrochemical sensors for the detection of HbA1c
- Published
- 2021
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43. The Key Role of Magnetic Resonance Imaging in the Detection of Neurodegenerative Diseases-Associated Biomarkers: A Review
- Author
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Ke-Ru Li, An-Guo Wu, Yong Tang, Xiao-Peng He, Chong-Lin Yu, Jian-Ming Wu, Guang-Qiang Hu, and Lu Yu
- Subjects
Cellular and Molecular Neuroscience ,Neurology ,Alzheimer Disease ,Neuroscience (miscellaneous) ,Humans ,Neurodegenerative Diseases ,Parkinson Disease ,Magnetic Resonance Imaging ,Biomarkers - Abstract
Neurodegenerative diseases (NDs), including chronic disease such as Alzheimer's disease, Parkinson's disease, Huntington's disease, and multiple sclerosis, and acute diseases like traumatic brain injury and ischemic stroke are characterized by progressive degeneration, brain tissue damage and loss of neurons, accompanied by behavioral and cognitive dysfunctions. So far, there are no complete cures for NDs; thus, early and timely diagnoses are essential and beneficial to patients' treatment. Magnetic resonance imaging (MRI) has become one of the advanced medical imaging techniques widely used in the clinical examination of NDs due to its non-invasive diagnostic value. In this review, research published in English in current decade from PubMed electronic database on the use of MRI to detect specific biomarkers of NDs was collected, summarized, and discussed, which provides valuable suggestions for the early diagnosis, prevention, and treatment of NDs in the clinic.
- Published
- 2021
44. Metal-organic frameworks (MOFs) as host materials for the enhanced delivery of biomacromolecular therapeutics
- Author
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Xiao-Peng He, Ling Zhu, Tony D. James, Pei-Hong Tong, Yi Zang, and Jia Li
- Subjects
Pore size ,Computer science ,fungi ,Metals and Alloys ,Nanotechnology ,General Chemistry ,Catalysis ,Additional research ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Disease therapy ,Drug Delivery Systems ,Pharmaceutical Preparations ,Drug delivery ,Materials Chemistry ,Ceramics and Composites ,Drug release ,Delivery system ,Metal-Organic Frameworks - Abstract
Biomacromolecular drugs have become an important class of therapeutic agents for the treatment of human diseases. Considering their high propensity for being degraded in the human body, the choice of an appropriate delivery system is key to ensure the therapeutic efficacy of biomacromolecular drugs in vivo. As an emerging class of supramolecular "host" materials, metal-organic frameworks (MOFs) exhibit advantages in terms of the tunability of pore size, encapsulation efficiency, controllable drug release, simplicity in surface functionalization and good biocompatibility. As a result, MOF-based host-guest systems have been extensively developed as a new class of flexible and powerful platform for the delivery of therapeutic biomacromolecules. In this review, we summarize current research progress in the synthesis of MOFs as delivery materials for a variety of biomacromolecules. Firstly, we briefly introduce the advances made in the use of biomacromolecular drugs for disease therapy and the types of commonly used clinical delivery systems. We then describe the advantages of using MOFs as delivery materials. Secondly, the strategies for the construction of MOF-encapsulated biomacromolecules (Biomacromolecules@MOFs) and the release mechanisms of the therapeutics are categorized. Thirdly, the application of MOFs to deliver different types of biomacromolecules (e.g., antigens/antibodies, enzymes, therapeutic proteins, DNA/RNA, polypeptides, and polysaccharides) for the treatment of various human diseases based on immunotherapy, gene therapy, starvation therapy and oxidation therapy is summarized. Finally, the remaining challenges and available opportunities for MOFs as drug delivery systems are outlined, which we anticipate will encourage additional research efforts directed towards developing Biomacromolecules@MOFs systems for biomedical applications.
- Published
- 2021
45. Near-Infrared Light-Triggered Bacterial Eradication Using a Nanowire Nanocomposite of Graphene Nanoribbons and Chitosan-Coated Silver Nanoparticles
- Author
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Xiao-Peng He, Guo-Rong Chen, Bin Zhang, Xi-Le Hu, Tony D. James, Yiyong Mai, Hui-Qi Gan, Ming Zhou, Fugui Xu, and Qiang Hu
- Subjects
silver nanoparticles ,Materials science ,Nanocomposite ,nanocomposite ,Graphene ,Nanowire ,Nanotechnology ,macromolecular substances ,General Chemistry ,Photothermal therapy ,Bacterial growth ,Silver nanoparticle ,law.invention ,Chitosan ,Chemistry ,chemistry.chemical_compound ,chemistry ,law ,antibiotic ,chitosan ,QD1-999 ,Graphene nanoribbons ,Original Research ,graphene nanoribbons - Abstract
Bacterial infection is a major threat to human health. However, many antibacterial agents currently used are severely limited due to drug-resistance, and the development of side effects. Herein, we have developed a non-antibiotic nanocomposite consisting of chitosan (ChS) coated silver nanoparticles (AgNPs) and graphene nanoribbon (GNR)-based nanowires for light-triggered eradication of bacteria. The presence of AgNP/ChS significantly enhanced the interactions of the GNR nanowires with Pseudomonas aeruginosa, a clinically common Gram-negative bacterium. Which enables the highly effective photothermal eradication of bacteria by GNR upon near-infrared light irradiation. The nanocomposite was shown to be applicable for the light-triggered eradication of bacterial biofilms and the inhibition of bacterial growth on medical patches used for abdominal-wall hernia surgery.
- Published
- 2021
46. Correction: Fluorescent glycoconjugates and their applications
- Author
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Xiao-Peng He, Baptiste Thomas, Kai-Cheng Yan, Marion Donnier-Maréchal, Xi-Le Hu, Sébastien Vidal, and Guo-Rong Chen
- Subjects
chemistry.chemical_classification ,Chemistry ,Glycoconjugate ,General Chemistry ,Computational biology ,Fluorescence - Abstract
Correction for ‘Fluorescent glycoconjugates and their applications’ by Baptiste Thomas et al., Chem. Soc. Rev., 2020, 49, 593–641, DOI: 10.1039/C8CS00118A.
- Published
- 2021
47. Long-Wavelength AIE-Based Fluorescent Probes for Mitochondria-Targeted Imaging and Photodynamic Therapy of Hepatoma Cells
- Author
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Ling Zhu, Fang Zhou Xu, Xing Yu Ma, Chengyun Wang, Xiao-Peng He, Hai Hao Han, Yi Zang, Jia Li, Dan Zhao, and Tony D. James
- Subjects
theranostic probe ,Carcinoma, Hepatocellular ,Chemistry(all) ,photosensitizer ,medicine.medical_treatment ,Biomedical Engineering ,Photodynamic therapy ,Mitochondrion ,photodynamic therapy (PDT) ,Biomaterials ,SDG 3 - Good Health and Well-being ,Cell Line, Tumor ,medicine ,Humans ,Photosensitizer ,Fluorescent Dyes ,Biochemistry, medical ,Photosensitizing Agents ,Chemistry ,Liver Neoplasms ,Biochemistry (medical) ,General Chemistry ,Fluorescence ,Mitochondria ,mitochondria ,Long wavelength ,Photochemotherapy ,Biophysics ,aggregation-induced emission (AIE) ,Mitochondria targeted - Abstract
With this research, we have developed two long-wavelength theranostic probes (DCMT and DCMC) with aggregation-induced emission (AIE)-based properties for image-guided photodynamic therapy (PDT) of hepatoma cells. Introduction of a triphenylamine or carbazole group to a dicyanomethylene-4H-pyran dye with long-wavelength fluorescence emission produces the AIE-based probes, which were subsequently modified with triphenyl-phosphonium cation for actively targeting the mitochondria of hepatoma cells. Solution-based experiments show that the probes exhibit a mixed photophysical mechanism of twisted-intramolecular charge transfer and AIE at different aggregation states. The molecular aggregation of the probes also leads to an enhanced ability for oxygen photosensitization, suggesting their potential for PDT of cancer cells. Our subsequent cell-based assays show that the probes localize in the mitochondria of hepatoma cells and the use of light leads to cell death through the intracellular production of reactive oxygen species.
- Published
- 2021
48. Dual-channel fluorescent probe for the simultaneous monitoring of peroxynitrite and adenosine-5’-triphosphate in cellular applications
- Author
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Xiao-Peng He, Jihong Liu, Tony D. James, Yonggang Yang, Jia Li, Ping Li, Chusen Huang, Luling Wu, Bo Tang, Xue Tian, Steven D. Bull, Yi Zhang, Yang Wang, Adam C. Sedgwick, Fang Huang, Jonathan L. Sessler, Hua Zhang, Han-Min Wang, Hai-Hao Han, and Robin R. Groleau
- Subjects
inorganic chemicals ,chemistry.chemical_classification ,Reactive oxygen species ,Oligomycin ,ATP synthase ,biology ,Pinacol ,Fluorescence ,Rhodamine ,chemistry.chemical_compound ,chemistry ,cardiovascular system ,biology.protein ,Biophysics ,Peroxynitrite ,Reactive nitrogen species - Abstract
The concentrations of ATP and ONOO− have been correlated with the progression a number of diseases including ischemia-reperfusion injury and drug-induced liver injury. Here, we report the development of fluorescent probe, ATP-LW, which enables the simultaneous detection of ONOO− and ATP. ONOO− selectively oxidises the boronate pinacol ester of ATP-LW, to afford the fluorescent 4-hydroxy-1,8-naphthalimide product NA-OH (λex = 450 nm, λem = 562 nm or λex = 488 nm, λem = 568 nm). While, the binding of ATP to ATP-LW induces the spirolactam ring opening of rhodamine to afford a highly emissive product (λex = 520 nm, λem = 587 nm). Due to the differences in emission between the ONOO− and ATP products, ATP-LW exhibits the unique ability to image ONOO− levels in the green channel (λex = 488 nm, λem = 500-575 nm) and ATP concentrations using the red channel (λex = 514 nm, λem = 575-650 nm). This was demonstrated using hepatocytes (HL-7702 cells) in cellular imaging experiments. The treatment of HL-7702 cell line with oligomycin A (an inhibitor of ATP synthase) resulted in a reduction of ATP in the red channel and increase in ONOO− green channel. While, the presence of SIN-1 (an exogenous ONOO− donor) results in an increase of ONOO−, and decrease in ATP. Significantly, when HL-7702 cells were treated with acetaminophen as a biological model for drug-induced liver injury, an increase in ONOO− green and decrease in ATP red channel fluorescence was observed. These results illustrate the utility of ATP-LW as a chemical tool to simultaneously monitor ATP and ONOO− concentrations in cellular-based applications.
- Published
- 2021
49. A General Strategy to the Intracellular Sensing of Glycosidases using AIE-Based Glycoclusters
- Author
-
Guo-Rong Chen, Hai-Hao Han, Lei Dong, Min-Yu Zhang, Xiao-Peng He, Sébastien Vidal, Jia Li, Yi Zang, Beijing Institute of Technology (BIT), Molécules de Communication et Adaptation des Micro-Organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), East China University of Science and Technology, Department Institute of Precision Optical Engineering, Tongji University, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), and Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
- Subjects
chemistry.chemical_classification ,Fluorescence-lifetime imaging microscopy ,Glycosylation ,Fluorophore ,Quenching (fluorescence) ,010405 organic chemistry ,Glycoconjugate ,Glycobiology ,General Chemistry ,Tetraphenylethylene ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences ,3. Good health ,Chemistry ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Click chemistry ,[CHIM]Chemical Sciences - Abstract
Glycosidases, which are the enzymes responsible for the removal of residual monosaccharides from glycoconjugates, are involved in many different biological and pathological events. The ability to detect sensitively the activity and spatiotemporal distribution of glycosidases in cells will provide useful tools for disease diagnosis. However, the currently developed fluorogenic probes for glycosidases are generally based on the glycosylation of the phenol group of a donor–acceptor type fluorogen. This molecular scaffold has potential drawbacks in terms of substrate scope, sensitivity because of aggregation-caused quenching (ACQ), and the inability for long-term cell tracking. Here, we developed glycoclusters characterized by aggregation-induced emission (AIE) properties as a general platform for the sensing of a variety of glycosidases. To overcome the low chemical reactivity associated with phenol glycosylation, here we developed an AIE-based scaffold, which is composed of tetraphenylethylene conjugated with dicyanomethylene-4H-pyran (TPE–DCM) with a red fluorescence emission. Subsequently, a pair of dendritic linkages was introduced to both sides of the fluorophore, to which six copies of monosaccharides (d-glucose, d-galactose or l-fucose) were introduced through azide–alkyne click chemistry. The resulting AIE-active glycoclusters were shown to be capable of (1) fluorogenic sensing of a diverse range of glycosidases including β-d-galactosidase, β-d-glucosidase and α-l-fucosidase through the AIE mechanism, (2) fluorescence imaging of the endogenous glycosidase activities in healthy and cancer cells, and during cell senescence, and (3) glycosidase-activated, long-term imaging of cells. The present study provides a general strategy to the functional, in situ imaging of glycosidase activities through the multivalent display of sugar epitopes of interest onto properly designed AIE-active fluorogens., We report a general strategy for the fluorogenic sensing of glycosidases in cells based on aggregation-induced emission of glycoclusters.
- Published
- 2021
50. Dermoid cyst of the left upper limb: A common case in a rare site
- Author
-
Wen Xiao, Dong Chen, and Xiao-Peng He
- Subjects
Upper Extremity ,Humans ,General Medicine ,Dermoid Cyst - Published
- 2021
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