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The positron, the antiparticle of the electron, predicted by Dirac in 1931 and discovered by Anderson in 1933, plays a key role in many scientific and everyday endeavours. Notably, the positron is a constituent of antihydrogen, the only long-lived neutral antimatter bound state that can currently be synthesized at low energy, presenting a prominent system for testing fundamental symmetries with high precision. Here, we report on the use of laser cooled Be+ ions to sympathetically cool a large and dense plasma of positrons to directly measured temperatures below 7 K in a Penning trap for antihydrogen synthesis. This will likely herald a significant increase in the amount of antihydrogen available for experimentation, thus facilitating further improvements in studies of fundamental symmetries., Positrons are key to the production of cold antihydrogen. Here the authors report the sympathetic cooling of positrons by interacting them with laser-cooled Be+ ions resulting in a three-fold reduction of the temperature of positrons for antihydrogen synthesis.

The ALPHA collaboration has successfully demonstrated the production and the confinement of cold antihydrogen, $\overline{\mathrm{H}}$. An analysis of trapping data allowed a stringent limit to be placed on the electric charge of the simplest antiatom. Charge neutrality of matter is known to a very high precision, hence a neutrality limit of $\overline{\mathrm{H}}$ provides a test of CPT invariance. The experimental technique is based on the measurement of the deflection of putatively charged $\overline{\mathrm{H}}$ in an electric field. The tendency for trapped $\overline{\mathrm{H}}$ atoms to be displaced by electrostatic fields is measured and compared to the results of a detailed simulation of $\overline{\mathrm{H}}$ dynamics in the trap. An extensive survey of the systematic errors is performed, with particular attention to those due to the silicon vertex detector, which is the device used to determine the $\overline{\mathrm{H}}$ annihilation position. The limit obtained on the charge of the $\overline{\mathrm{H}}$ atom is \mbox{$ Q = (-1.3\pm1.8\pm0.4)\times10^{-8}$}, representing the first precision measurement with $\overline{\mathrm{H}}$., Comment: 5 pages, 3 figures

At the historic Shelter Island Conference on the Foundations of Quantum Mechanics in 1947, Willis Lamb reported an unexpected feature in the fine structure of atomic hydrogen: a separation of the 2S

The photon—the quantum excitation of the electromagnetic field—is massless but carries momentum. A photon can therefore exert a force on an object upon collision1. Slowing the translational motion of atoms and ions by application of such a force2,3, known as laser cooling, was first demonstrated 40 years ago4,5. It revolutionized atomic physics over the following decades6–8, and it is now a workhorse in many fields, including studies on quantum degenerate gases, quantum information, atomic clocks and tests of fundamental physics. However, this technique has not yet been applied to antimatter. Here we demonstrate laser cooling of antihydrogen9, the antimatter atom consisting of an antiproton and a positron. By exciting the 1S–2P transition in antihydrogen with pulsed, narrow-linewidth, Lyman-α laser radiation10,11, we Doppler-cool a sample of magnetically trapped antihydrogen. Although we apply laser cooling in only one dimension, the trap couples the longitudinal and transverse motions of the anti-atoms, leading to cooling in all three dimensions. We observe a reduction in the median transverse energy by more than an order of magnitude—with a substantial fraction of the anti-atoms attaining submicroelectronvolt transverse kinetic energies. We also report the observation of the laser-driven 1S–2S transition in samples of laser-cooled antihydrogen atoms. The observed spectral line is approximately four times narrower than that obtained without laser cooling. The demonstration of laser cooling and its immediate application has far-reaching implications for antimatter studies. A more localized, denser and colder sample of antihydrogen will drastically improve spectroscopic11–13 and gravitational14 studies of antihydrogen in ongoing experiments. Furthermore, the demonstrated ability to manipulate the motion of antimatter atoms by laser light will potentially provide ground-breaking opportunities for future experiments, such as anti-atomic fountains, anti-atom interferometry and the creation of antimatter molecules., The successful laser cooling of trapped antihydrogen, the antimatter atom formed by an antiproton and a positron (anti-electron), is reported.

A primary goal of the ALPHA experiment at CERN is to perform precise tests of CPT symmetry. Here, we report on the significant progress made in recent years on antihydrogen spectroscopy and the outlook for the future., Presented at the Eighth Meeting on CPT and Lorentz Symmetry, Bloomington, Indiana, May 12-16, 2019

The local magnetic field in a Penning-Malmberg trap is found by measuring the temperatures that result when electron plasmas are illuminated by microwaves pulses. Multiple heating resonances are observed as the pulse frequencies are swept. The many resonances are due to electron bounce and plasma rotation sidebands. The heating peak corresponding to the cyclotron frequency resonance is identified to determine the magnetic field. A new method for quickly preparing low density electron plasmas for destructive temperature measurements enables a rapid and automated scan of microwave frequencies. This technique can determine the magnetic field to high precision, obtaining an absolute accuracy better than $1\,\mathrm{ppm}$, and a relative precision of $26\,\mathrm{ppb}$. One important application is in situ magnetometry for antihydrogen-based tests of charge-parity-time symmetry and of the weak equivalence principle

In 1906, Theodore Lyman discovered his eponymous series of transitions in the extreme-ultraviolet region of the atomic hydrogen spectrum1,2. The patterns in the hydrogen spectrum helped to establish the emerging theory of quantum mechanics, which we now know governs the world at the atomic scale. Since then, studies involving the Lyman-α line—the 1S–2P transition at a wavelength of 121.6 nanometres—have played an important part in physics and astronomy, as one of the most fundamental atomic transitions in the Universe. For example, this transition has long been used by astronomers studying the intergalactic medium and testing cosmological models via the so-called ‘Lyman-α forest’3 of absorption lines at different redshifts. Here we report the observation of the Lyman-α transition in the antihydrogen atom, the antimatter counterpart of hydrogen. Using narrow-line-width, nanosecond-pulsed laser radiation, the 1S–2P transition was excited in magnetically trapped antihydrogen. The transition frequency at a field of 1.033 tesla was determined to be 2,466,051.7 ± 0.12 gigahertz (1σ uncertainty) and agrees with the prediction for hydrogen to a precision of 5 × 10−8. Comparisons of the properties of antihydrogen with those of its well-studied matter equivalent allow precision tests of fundamental symmetries between matter and antimatter. Alongside the ground-state hyperfine4,5 and 1S–2S transitions6,7 recently observed in antihydrogen, the Lyman-α transition will permit laser cooling of antihydrogen8,9, thus providing a cold and dense sample of anti-atoms for precision spectroscopy and gravity measurements10. In addition to the observation of this fundamental transition, this work represents both a decisive technological step towards laser cooling of antihydrogen, and the extension of antimatter spectroscopy to quantum states possessing orbital angular momentum., The observation of the 1S–2P Lyman-α transition in the antihydrogen atom, the antimatter counterpart of hydrogen, is reported.

Antihydrogen, a positron bound to an antiproton, is the simplest anti-atom. Its structure and properties are expected to mirror those of the hydrogen atom. Prospects for precision comparisons of the two, as tests of fundamental symmetries, are driving a vibrant programme of research. In this regard, a limiting factor in most experiments is the availability of large numbers of cold ground state antihydrogen atoms. Here, we describe how an improved synthesis process results in a maximum rate of 10.5 ± 0.6 atoms trapped and detected per cycle, corresponding to more than an order of magnitude improvement over previous work. Additionally, we demonstrate how detailed control of electron, positron and antiproton plasmas enables repeated formation and trapping of antihydrogen atoms, with the simultaneous retention of atoms produced in previous cycles. We report a record of 54 detected annihilation events from a single release of the trapped anti-atoms accumulated from five consecutive cycles., Antihydrogen studies are important in testing the fundamental principles of physics but producing antihydrogen in large amounts is challenging. Here the authors demonstrate an efficient and high-precision method for trapping and stacking antihydrogen by using controlled plasma.

International audience; The simultaneous control of the density and particle number of non-neutral plasmas confined in Penning-Malmberg traps is demonstrated. Control is achieved by setting the plasma’s density by applying a rotating electric field while simultaneously fixing its axial potential via evaporative cooling. This novel method is particularly useful for stabilizing positron plasmas, as the procedures used to collect positrons from radioactive sources typically yield plasmas with variable densities and particle numbers; it also simplifies optimization studies that require plasma parameter scans. The reproducibility achieved by applying this technique to the positron and electron plasmas used by the ALPHA antihydrogen experiment at CERN, combined with other developments, contributed to a 10-fold increase in the antiatom trapping rate.

In 1928, Dirac published an equation1 that combined quantum mechanics and special relativity. Negative-energy solutions to this equation, rather than being unphysical as initially thought, represented a class of hitherto unobserved and unimagined particles—antimatter. The existence of particles of antimatter was confirmed with the discovery of the positron2 (or anti-electron) by Anderson in 1932, but it is still unknown why matter, rather than antimatter, survived after the Big Bang. As a result, experimental studies of antimatter3–7, including tests of fundamental symmetries such as charge–parity and charge–parity–time, and searches for evidence of primordial antimatter, such as antihelium nuclei, have high priority in contemporary physics research. The fundamental role of the hydrogen atom in the evolution of the Universe and in the historical development of our understanding of quantum physics makes its antimatter counterpart—the antihydrogen atom—of particular interest. Current standard-model physics requires that hydrogen and antihydrogen have the same energy levels and spectral lines. The laser-driven 1S–2S transition was recently observed8 in antihydrogen. Here we characterize one of the hyperfine components of this transition using magnetically trapped atoms of antihydrogen and compare it to model calculations for hydrogen in our apparatus. We find that the shape of the spectral line agrees very well with that expected for hydrogen and that the resonance frequency agrees with that in hydrogen to about 5 kilohertz out of 2.5 × 1015 hertz. This is consistent with charge–parity–time invariance at a relative precision of 2 × 10−12—two orders of magnitude more precise than the previous determination8—corresponding to an absolute energy sensitivity of 2 × 10−20 GeV., The shape of the spectral line and the resonance frequency of the 1S–2S transition in antihydrogen agree very well with those of hydrogen.

The observation of hyperfine structure in atomic hydrogen by Rabi and co-workers1,2,3 and the measurement4 of the zero-field ground-state splitting at the level of seven parts in 1013 are important achievements of mid-twentieth-century physics. The work that led to these achievements also provided the first evidence for the anomalous magnetic moment of the electron5,6,7,8, inspired Schwinger’s relativistic theory of quantum electrodynamics9,10 and gave rise to the hydrogen maser11, which is a critical component of modern navigation, geo-positioning and very-long-baseline interferometry systems. Research at the Antiproton Decelerator at CERN by the ALPHA collaboration extends these enquiries into the antimatter sector. Recently, tools have been developed that enable studies of the hyperfine structure of antihydrogen12—the antimatter counterpart of hydrogen. The goal of such studies is to search for any differences that might exist between this archetypal pair of atoms, and thereby to test the fundamental principles on which quantum field theory is constructed. Magnetic trapping of antihydrogen atoms13,14 provides a means of studying them by combining electromagnetic interaction with detection techniques that are unique to antimatter12,15. Here we report the results of a microwave spectroscopy experiment in which we probe the response of antihydrogen over a controlled range of frequencies. The data reveal clear and distinct signatures of two allowed transitions, from which we obtain a direct, magnetic-field-independent measurement of the hyperfine splitting. From a set of trials involving 194 detected atoms, we determine a splitting of 1,420.4 ± 0.5 megahertz, consistent with expectations for atomic hydrogen at the level of four parts in 104. This observation of the detailed behaviour of a quantum transition in an atom of antihydrogen exemplifies tests of fundamental symmetries such as charge–parity–time in antimatter, and the techniques developed here will enable more-precise such tests.

© 2016 Macmillan Publishers Limited. All rights reserved. Antimatter continues to intrigue physicists because of its apparent absence in the observable Universe. Current theory requires that matter and antimatter appeared in equal quantities after the Big Bang, but the Standard Model of particle physics offers no quantitative explanation for the apparent disappearance of half the Universe. It has recently become possible to study trapped atoms - of antihydrogen to search for possible, as yet unobserved, differences in the physical behaviour of matter and antimatter. Here we consider the charge neutrality of the antihydrogen atom. By applying stochastic acceleration to trapped antihydrogen atoms, we determine an experimental bound on the antihydrogen charge, Qe, of |Q| < 0.71 parts per billion (one standard deviation), in which e is the elementary charge. This bound is a factor of 20 less than that determined from the best previous measurement of the antihydrogen charge. The electrical charge of atoms and molecules of normal matter is known to be no greater than about 10-21e for a diverse range of species including H2, He and SF6. Charge-parity-time symmetry and quantum anomaly cancellation demand that the charge of antihydrogen be similarly small. Thus, our measurement constitutes an improved limit and a test of fundamental aspects of the Standard Model. If we assume charge superposition and use the best measured value of the antiproton charge, then we can place a new limit on the positron charge anomaly (the relative difference between the positron and elementary charge) of about one part per billion (one standard deviation), a 25-fold reduction compared to the current best measurement.

Magnetically trapped antihydrogen atoms at low temperatures were used to test the charge neutrality of this antiatomic system, by precisely placing a limit to its electrical charge. A new method for measuring the gravitational interaction of antimatter was also developed, and prospects for making a precise measurement of this interaction are discussed. Finally, progress towards a precise, direct test of CPT symmetry by laser spectroscopy of antihydrogen is presented.

The 1S–2S transition in magnetically trapped atoms of antihydrogen is observed, and its frequency is shown to be consistent with that expected for hydrogen. Testing to high precision whether matter behaves like antimatter could provide clues to one of the biggest puzzles in modern physics: why does the observable Universe consist almost entirely of matter? After all, the Standard Model predicts that after the Big Bang the Universe should have been made up of equal amounts of matter and antimatter. Antimatter is difficult to produce and characterize because it annihilates when it comes in contact with matter. But recent advances at CERN's Antiproton Decelerator have allowed researchers to trap and measure both antiprotons and antihydrogen. Now the ALPHA Collaboration at CERN reports the first spectroscopic characterization of antihydrogen, exciting the 1S to 2S transition with laser light. The transition frequency is consistent with that of hydrogen. The spectrum of ordinary hydrogen has been characterized to extremely high precision, so improvements in antihydrogen spectroscopy will yield highly sensitive tests of matter–antimatter symmetry. The spectrum of the hydrogen atom has played a central part in fundamental physics over the past 200 years. Historical examples of its importance include the wavelength measurements of absorption lines in the solar spectrum by Fraunhofer, the identification of transition lines by Balmer, Lyman and others, the empirical description of allowed wavelengths by Rydberg, the quantum model of Bohr, the capability of quantum electrodynamics to precisely predict transition frequencies, and modern measurements of the 1S–2S transition by Hansch1 to a precision of a few parts in 1015. Recent technological advances have allowed us to focus on antihydrogen—the antimatter equivalent of hydrogen2,3,4. The Standard Model predicts that there should have been equal amounts of matter and antimatter in the primordial Universe after the Big Bang, but today’s Universe is observed to consist almost entirely of ordinary matter. This motivates the study of antimatter, to see if there is a small asymmetry in the laws of physics that govern the two types of matter. In particular, the CPT (charge conjugation, parity reversal and time reversal) theorem, a cornerstone of the Standard Model, requires that hydrogen and antihydrogen have the same spectrum. Here we report the observation of the 1S–2S transition in magnetically trapped atoms of antihydrogen. We determine that the frequency of the transition, which is driven by two photons from a laser at 243 nanometres, is consistent with that expected for hydrogen in the same environment. This laser excitation of a quantum state of an atom of antimatter represents the most precise measurement performed on an anti-atom. Our result is consistent with CPT invariance at a relative precision of about 2 × 10−10.

The ALPHA project is an international collaboration, based at CERN, with the experimental goal of performing precision spectroscopic measurements on antihydrogen. As part of this endeavor, the ALPHA experiment includes a silicon tracking detector. This detector consists of a three-layer array of silicon modules surrounding the antihydrogen trapping region of the ALPHA apparatus. Using this device, the antihydrogen annihilation position can be determined with a spatial resolution of better than 5 mm. Knowledge of the annihilation distribution was a critical component in the recently successful antihydrogen trapping effort. This paper will describe the methods used to reconstruct annihilation events in the ALPHA detector. Particular attention will be given to the description of the background rejection criteria.

Precision comparisons of hyperfine intervals in atomic hydrogen and antihydrogen are expected to yield experimental tests of the CPT theorem. The CERN-based ALPHA collaboration has initiated a program of study focused on microwave spectroscopy of trapped ground-state antihydrogen atoms. This paper outlines some of the proposed experiments, and summarizes measurements that characterize microwave fields that have been injected into the ALPHA apparatus.

Precision spectroscopic comparison of hydrogen and antihydrogen holds the promise of a sensitive test of the Charge-Parity-Time theorem and matter-antimatter equivalence. The clearest path towards realising this goal is to hold a sample of antihydrogen in an atomic trap for interrogation by electromagnetic radiation. Achieving this poses a huge experimental challenge, as state-of-the-art magnetic-minimum atom traps have well depths of only ∼1 T (∼0.5 K for ground state antihydrogen atoms). The atoms annihilate on contact with matter and must be ‘born’ inside the magnetic trap with low kinetic energies. At the ALPHA experiment, antihydrogen atoms are produced from antiprotons and positrons stored in the form of non-neutral plasmas, where the typical electrostatic potential energy per particle is on the order of electronvolts, more than 104 times the maximum trappable kinetic energy. In November 2010, ALPHA published the observation of 38 antiproton annihilations due to antihydrogen atoms that had been trapped for at least 172 ms and then released—the first instance of a purely antimatter atomic system confined for any length of time (Andresen et al., Nature 468:673, 2010). We present a description of the main components of the ALPHA traps and detectors that were key to realising this result. We discuss how the antihydrogen atoms were identified and how they were discriminated from the background processes. Since the results published in Andresen et al. (Nature 468:673, 2010), refinements in the antihydrogen production technique have allowed many more antihydrogen atoms to be trapped, and held for much longer times. We have identified antihydrogen atoms that have been trapped for at least 1,000 s in the apparatus (Andresen et al., Nature Physics 7:558, 2011). This is more than sufficient time to interrogate the atoms spectroscopically, as well as to ensure that they have relaxed to their ground state.

In efforts to trap antihydrogen, a key problem is the vast disparity between the neutral trap energy scale (\(\sim\!50\,\upmu\mathrm{eV}\)), and the energy scales associated with plasma confinement and space charge (~1 eV). In order to merge charged particle species for direct recombination, the larger energy scale must be overcome in a manner that minimizes the initial antihydrogen kinetic energy. This issue motivated the development of a novel injection technique utilizing the inherent nonlinear nature of particle oscillations in our traps. We demonstrated controllable excitation of the center-of-mass longitudinal motion of a thermal antiproton plasma using a swept-frequency autoresonant drive. When the plasma is cold, dense and highly collective in nature, we observe that the entire system behaves as a single-particle nonlinear oscillator, as predicted by a recent theory. In contrast, only a fraction of the antiprotons in a warm or tenuous plasma can be similarly excited. Antihydrogen was produced and trapped by using this technique to drive antiprotons into a positron plasma, thereby initiating atomic recombination. The nature of this injection overcomes some of the difficulties associated with matching the energies of the charged species used to produce antihydrogen.

The structure and torsional properties of oxalyl chloride fluoride in the gas phase have been measured by electron diffraction at temperatures of 22, 81, 158, and 310 °C. The molecule may be regarded as a hybrid of oxalyl chloride and oxalyl fluoride. Since the former exists as a more stable periplanar anti form (ϕ = 180°) in equilibrium with a less stable gauche form (ϕ ≃ 60°) and the latter as an equilibrium between two periplanar forms, anti and syn, the second form of oxalyl chloride fluoride is an interesting question. It was found to be gauche. The system was modeled as two rotational conformers related by a potential of the form 2V = V(1)(1 + cos ϕ) - V(2)(1 - cos 2ϕ) + V(3)(1 + cos 3ϕ). The anti/gauche bond distances and bond angles (r(g)/Angstroms, ∠(α)/degrees) with estimated 2σ uncertainties at 22 °C arer(C═O)= 1.183(2)/1.182(2), Δr(C═O) = 0.003(6)/0.002(6) (assumed from theory), r(C-F) = 1.329(3)/1.335(3), r(C-Cl) = 1.738(2)/1.753(2), ∠(C-C-Cl) = 112.0(3)/111.9(3), ∠(C-C═O3) = 123.0(4)/123.2(4), ∠(O═C-Cl) = 125.0(2)/1.249(2), ∠(O═C-F) = 123.0(3)/125.1(3), and ∠(Cl-C-C-F) = 180.0/59.8. The variation of composition with temperature afforded a determination of the standard enthalpy and entropy of the reaction anti → gauche. The results are ΔH° = 2.5(12) kcal/mol and ΔS° = -6.5(33) cal/(mol·K). The structures and equilibria are discussed.

Spectroscopy of antihydrogen has the potential to yield high-precision tests of the CPT theorem and shed light on the matter-antimatter imbalance in the Universe. The ALPHA antihydrogen trap at CERN's Antiproton Decelerator aims to prepare a sample of antihydrogen atoms confined in an octupole-based Ioffe trap and to measure the frequency of several atomic transitions. We describe our techniques to directly measure the antiproton temperature and a new technique to cool them to below 10 K. We also show how our unique position-sensitive annihilation detector provides us with a highly sensitive method of identifying antiproton annihilations and effectively rejecting the cosmic-ray background., 10 pages, 5 figures

Antihydrogen spectroscopy promises precise tests of the symmetry of matter and antimatter, and can possibly offer new insights into the baryon asymmetry of the universe. Antihydrogen is, however, difficult to synthesize and is produced only in small quantities. The ALPHA collaboration is therefore pursuing a path towards trapping cold antihydrogen to permit the use of precision atomic physics tools to carry out comparisons of antihydrogen and hydrogen. ALPHA has addressed these challenges. Control of the plasma sizes has helped to lower the influence of the multipole field used in the neutral atom trap, and thus lowered the temperature of the created atoms. Finally, the first systematic attempt to identify trapped antihydrogen in our system is discussed. This discussion includes special techniques for fast release of the trapped anti-atoms, as well as a silicon vertex detector to identify antiproton annihilations. The silicon detector reduces the background of annihilations, including background from antiprotons that can be mirror trapped in the fields of the neutral atom trap. A description of how to differentiate between these events and those resulting from trapped antihydrogen atoms is also included.

We have observed a new mechanism for compression of a non-neutral plasma, where antiprotons embedded in an electron plasma are compressed by a rotating wall drive at a frequency close to the sum of the axial bounce and rotation frequencies. The radius of the antiproton cloud is reduced by up to a factor of 20 and the smallest radius measured is similar to 0.2 mm. When the rotating wall drive is applied to either a pure electron or pure antiproton plasma, no compression is observed in the frequency range of interest. The frequency range over which compression is evident is compared to the sum of the antiproton bounce frequency and the system's rotation frequency. It is suggested that bounce resonant transport is a likely explanation for the compression of antiproton clouds in this regime.

The molecular structure and composition of gaseous oxalyl fluoride (OXF) has been investigated by electron diffraction (GED) at nozzle-tip temperatures of −10, 149, and 219 °C. The GED data were augmented by molecular orbital calculations, and the analysis was aided by use of rotational constants from microwave (MW) spectroscopy. As in the other oxalyl halides, there are two stable species, of which the more stable is periplanar anti (i.e., trans). However, unlike these other halides in which the second form is gauche, the second form of oxalyl fluoride was known from MW work to be periplanar syn (i.e., cis). Our results are consistent with a mixture of trans and cis forms, and yield values for the structural parameters, the composition of the system at the three temperatures cited, and the thermodynamic quantities ΔG°, ΔH°, and ΔS° for the reaction trans → cis. Some trans/cis distances (rg/A) and angles (∠α/deg) at −10 °C are r(CO) = 1.178(2)/1.176(2), r(C−F) = 1.323(2)/1.328(2); r(C−C) = 1.533(3)/1.535(...

The molecular mechanisms by which Mesenchyme Homeobox 2 (Meox2) regulates the proliferation, differentiation and migration of vascular smooth muscle cells and cardiomyocytes are not known. The discovery of MEOX2 binding proteins will aid in understanding how MEOX2 functions as a regulator of these key cellular processes. To identify MEOX2 binding proteins, a yeast two-hybrid screen of a human heart cDNA library was performed using a deleted MEOX2 bait protein that does not contain the histidine/glutamine rich region (MEOX2deltaHQ). Eleven putative interacting proteins were identified including RING finger protein 10 (RNF10). In vitro pull-down assays and co-immunoprecipitation studies in mammalian cells further supported the yeast data demonstrating RNF10 bound to MEOX2. The minimal RNF10 binding region of MEOX2 was determined to be a central region between the histidine/glutamine rich domain and the homeodomain (amino acids 101-185). The amino terminal region of RNF10, containing the RING finger domain, was not essential for the binding to MEOX2. Our results also demonstrated that MEOX2 activation of the p21WAF1 promoter was enhanced by RNF10 co-expression.

The properties of antihydrogen are expected to be identical to those of hydrogen, and any differences would constitute a profound challenge to the fundamental theories of physics. The most commonly discussed antiatom-based tests of these theories are searches for antihydrogen-hydrogen spectral differences (tests of CPT (charge-parity-time) invariance) or gravitational differences (tests of the weak equivalence principle). Here we, the ALPHA Collaboration, report a different and somewhat unusual test of CPT and of quantum anomaly cancellation. A retrospective analysis of the influence of electric fields on antihydrogen atoms released from the ALPHA trap finds a mean axial deflection of 4.1±3.4 mm for an average axial electric field of 0.51 V mm−1. Combined with extensive numerical modelling, this measurement leads to a bound on the charge Qe of antihydrogen of Q=(−1.3±1.1±0.4) × 10−8. Here, e is the unit charge, and the errors are from statistics and systematic effects., Fundamental theories do not predict a difference between the properties of matter and antimatter, but experimental tests of this are still in their infancy. To this end, this study analyses the effects of electric fields on antihydrogen atoms in the ALPHA trap to place a bound on the charge of antihydrogen.

We demonstrate a novel detection method for the cyclotron resonance frequency of an electron plasma in a Penning-Malmberg trap. With this technique, the electron plasma is used as an in situ diagnostic tool for measurement of the static magnetic field and the microwave electric field in the trap. The cyclotron motion of the electron plasma is excited by microwave radiation and the temperature change of the plasma is measured non-destructively by monitoring the plasma's quadrupole mode frequency. The spatially-resolved microwave electric field strength can be inferred from the plasma temperature change and the magnetic field is found through the cyclotron resonance frequency. These measurements were used extensively in the recently reported demonstration of resonant quantum interactions with antihydrogen. We demonstrate a novel detection method for the cyclotron resonance frequency of an electron plasma in a Penning–Malmberg trap. With this technique, the electron plasma is used as an in situ diagnostic tool for the measurement of the static magnetic field and the microwave electric field in the trap. The cyclotron motion of the electron plasma is excited by microwave radiation and the temperature change of the plasma is measured non-destructively by monitoring the plasma's quadrupole mode frequency. The spatially resolved microwave electric field strength can be inferred from the plasma temperature change and the magnetic field is found through the cyclotron resonance frequency. These measurements were used extensively in the recently reported demonstration of resonant quantum interactions with antihydrogen. We demonstrate a novel detection method for the cyclotron resonance frequency of an electron plasma in a Penning-Malmberg trap. With this technique, the electron plasma is used as an in situ diagnostic tool for measurement of the static magnetic field and the microwave electric field in the trap. The cyclotron motion of the electron plasma is excited by microwave radiation and the temperature change of the plasma is measured non-destructively by monitoring the plasma's quadrupole mode frequency. The spatially-resolved microwave electric field strength can be inferred from the plasma temperature change and the magnetic field is found through the cyclotron resonance frequency. These measurements were used extensively in the recently reported demonstration of resonant quantum interactions with antihydrogen.

Long term magnetic confinement of antihydrogen atoms has recently been demonstrated by the ALPHA collaboration at CERN, opening the door to a range of experimental possibilities. Of particular interest is a measurement of the antihydrogen spectrum. A precise comparison of the spectrum of antihydrogen with that of hydrogen would be an excellent test of CPT symmetry. One prime candidate for precision CPT tests is the ground-state hyperfine transition; measured in hydrogen to a precision of nearly one part in 1012. Effective execution of such an experiment with trapped antihydrogen requires precise knowledge of the magnetic environment. Here we present a solution that uses an electron plasma confined in the antihydrogen trapping region. The cyclotron resonance of the electron plasma is probed with microwaves at the cyclotron frequency and the subsequent heating of the electron plasma is measured through the plasma quadrupole mode frequency. Using this method, the minimum magnetic field of the neutral trap can be determined to within 4 parts in 104. This technique was used extensively in the recent demonstration of resonant interaction with the hyperfine levels of trapped antihydrogen atoms.

We describe the implementation of evaporative cooling of charged particles in the ALPHA apparatus. Forced evaporation has been applied to cold samples of antiprotons held in Malmberg-Penning traps. Temperatures on the order of 10 K were obtained, while retaining a significant fraction of the initial number of particles. We have developed a model for the evaporation process based on simple rate equations and applied it succesfully to the experimental data. We have also observed radial re-distribution of the clouds following evaporation, explained by simple conservation laws. We discuss the relevance of this technique for the recent demonstration of magnetic trapping of antihydrogen.

One of the goals of synthesizing and trapping antihydrogen is to study the validity of charge-parity-time symmetry through precision spectroscopy on the anti-atoms, but the trapping yield achieved in recent experiments must be significantly improved before this can be realized. Antihydrogen atoms are commonly produced by mixing antiprotons and positrons stored in a nested Penning-Malmberg trap, which was achieved in ALPHA by an autoresonant excitation of the antiprotons, injecting them into the positron plasma. In this work, a hybrid numerical model is developed to simulate antiproton and positron dynamics during the mixing process. The simulation is benchmarked against other numerical and analytic models, as well as experimental measurements. The autoresonant injection scheme and an alternative scheme are compared numerically over a range of plasma parameters which can be reached in current and upcoming antihydrogen experiments, and the latter scheme is seen to offer significant improvement in trapping yield as the number of available antiprotons increases. One of the goals of synthesizing and trapping antihydrogen is to study the validity of charge-parity-time symmetry through precision spectroscopy on the anti-atoms, but the trapping yield achieved in recent experiments must be significantly improved before this can be realized. Antihydrogen atoms are commonly produced by mixing antiprotons and positrons stored in a nested Penning-Malmberg trap, which was achieved in ALPHA by an autoresonant excitation of the antiprotons, injecting them into the positron plasma. In this work, a hybrid numerical model is developed to simulate antiproton and positron dynamics during the mixing process. The simulation is benchmarked against other numerical and analytic models, as well as experimental measurements. The autoresonant injection scheme and an alternative scheme are compared numerically over a range of plasma parameters which can be reached in current and upcoming antihydrogen experiments, and the latter scheme is seen to offer significant improvement in trapping yield as the number of available antiprotons increases.

Physicists have long wondered whether the gravitational interactions between matter and antimatter might be different from those between matter and itself. Although there are many indirect indications that no such differences exist and that the weak equivalence principle holds, there have been no direct, free-fall style, experimental tests of gravity on antimatter. Here we describe a novel direct test methodology; we search for a propensity for antihydrogen atoms to fall downward when released from the ALPHA antihydrogen trap. In the absence of systematic errors, we can reject ratios of the gravitational to inertial mass of antihydrogen >75 at a statistical significance level of 5%; worst-case systematic errors increase the minimum rejection ratio to 110. A similar search places somewhat tighter bounds on a negative gravitational mass, that is, on antigravity. This methodology, coupled with ongoing experimental improvements, should allow us to bound the ratio within the more interesting near equivalence regime., One intriguing question about antimatter that is yet to be directly answered is whether or not it behaves exactly the same as matter under gravity. Here, a direct experimental method is presented to measure the ratio of inertial to gravitational mass for antihydrogen under free-fall conditions.

A utility index for otariid seals, based on the average flesh weight per skeletal portion of two California sea lions (Zalophus californianus), is presented. Relative to the recently derived phocid seal index, the otariid seal index has much higher values for the cervical vertebrae and forelimb elements, and lower values for the thoracic and lumbar vertebrae and pelves. Application to the archaeological otariid assemblages from the Seal Rock site on the Oregon coast, and from the Point Mugu site on the California coast, demonstrates the usefulness of the otariid seal index, while at the same time underscoring the inapplicability of the phocid seal index to such assemblages.

A meat utility index for odontocetes (Cetacea), based on average flesh weight per skeletal portion of a harbour porpoise ( Phocoena phocoena ), is presented. The highest ranked portions are associated with the middle and posterior vertebral column, while the lowest ranked portions are associated with the flippers, head, and anterior part of the vertebral column. The index (including both meat utility values and modified meat utility values) is applied to archaeologically- and ethnoarchaeologically-derived cetacean bone assemblages. Results suggest that while the ease of edible sculp and meat removal may significantly skew expected bone element frequencies, the index (especially that based on modified meat utility values) is nevertheless a useful predictive tool for small, and to some extent medium-sized, cetaceans.

A membrane-based extraction process is described for the fractionation of citrus oils to produce an oil stream enriched in oxygenated components. The process relies on the high selectivity of cyclodextrins (CDs) to preferentially bind the desirable oxygenates. In the process, low-temperature citrus oil flows on one side of a nonporous membrane, and an aqueous CD solution flows on the other side of the membrane. Oxygenates diffuse through the water-swollen hydrophilic membrane and preferentially partition into the aqueous CD solution. The aqueous CD solution is then heated and circulated past a second membrane, where the CD/oxygenate complex dissociates and the liberated oxygenates diffuse into a citrus oil solution. Disassociated CD is recycled to the first membrane. Data are presented on the effect of temperature and CD type on the partitioning of oxygenates from orange oil into aqueous CD solutions. Operation of a pilot-scale test loop that capitalizes on the high selectivity of CDs and the temperature dependence of the CD binding is described for the production of oxygenate-enriched orange oil.

Over the last decades it has become routine to form beams of positrons and antiprotons and to use them to produce trapped samples of both species for a variety of purposes. Positrons can be captured efficiently, for instance using a buffer-gas system, and in such quantities to form dense, single component plasmas useful for antihydrogen formation. The latter is possible using developments of techniques for dynamically capturing and then cooling antiprotons ejected from the Antiproton Decelerator at CERN. The antiprotons can then be manipulated by cloud compression and evaporative cooling to form tailored plasmas. We will review recent advances that have allowed antihydrogen atoms to be confined for the first time in a shallow magnetic minimum neutral atom trap superimposed upon the region in which the antiparticles are held and mixed. A new mixing technique has been developed to help achieve this using autoresonant excitation of the centreofmass longitudinal motion of an antiproton cloud. This allows efficient antihydrogen formation without imparting excess energy to the antiprotons and helps enhance the probability of trapping the anti-atom.

The ALPHA experiment, located at CERN, aims to compare the properties of antihydrogen atoms with those of hydrogen atoms. The neutral antihydrogen atoms are trapped using an octupole magnetic trap. The trap region is surrounded by a three layered silicon detector used to reconstruct the antiproton annihilation vertices. This paper describes a method we have devised that can be used for reconstructing annihilation vertices with a good resolution and is more efficient than the standard method currently used for the same purpose.

The goal of the ALPHA experiment is the production, trapping and spectroscopy of antihydrogen. A direct comparison of the ground state hyperfine spectra in hydrogen and antihydrogen has the potential to be a high-precision test of CPT symmetry. We present a novel method for measuring the strength of a microwave field for hyperfine spectroscopy in a Penning trap. This method incorporates a non-destructive plasma diagnostic system based on electrostatic modes within an electron plasma. We also show how this technique can be used to measure the cyclotron resonance of the electron plasma, which can potentially serve as a non-destructive measurement of plasma temperature.

The ALPHA experiment has succeeded in trapping antihydrogen, a major milestone on the road to spectroscopic comparisons of antihydrogen with hydrogen. An annihilation vertex detector, which determines the time and position of antiproton annihilations, has been central to this achievement. This detector, an array of double-sided silicon microstrip detector modules arranged in three concentric cylindrical tiers, is sensitive to the passage of charged particles resulting from antiproton annihilation. This article describes the method used to reconstruct the annihilation location and to distinguish the annihilation signal from the cosmic ray background. Recent experimental results using this detector are outlined.

ALPHA is one of the experiments situated at CERN's Antiproton Decelerator (AD). A Silicon Vertex Detector (SVD) is placed to surround the ALPHA atom trap. The main purpose of the SVD is to detect and locate antiproton annihilation events by means of the emitted charged pions. The SVD system is presented with special focus given to the design, fabrication and performance of the modules.

Atoms made of a particle and an antiparticle are unstable, usually surviving less than a microsecond. Antihydrogen, made entirely of antiparticles, is believed to be stable, and it is this longevity that holds the promise of precision studies of matter-antimatter symmetry. We have recently demonstrated trapping of antihydrogen atoms by releasing them after a confinement time of 172 ms. A critical question for future studies is: how long can anti-atoms be trapped? Here we report the observation of anti-atom confinement for 1000 s, extending our earlier results by nearly four orders of magnitude. Our calculations indicate that most of the trapped anti-atoms reach the ground state. Further, we report the first measurement of the energy distribution of trapped antihydrogen which, coupled with detailed comparisons with simulations, provides a key tool for the systematic investigation of trapping dynamics. These advances open up a range of experimental possibilities, including precision studies of CPT symmetry and cooling to temperatures where gravitational effects could become apparent., 30 pages, 4 figures

Charges in cold, multiple-species, non-neutral plasmas separate radially by mass, forming centrifugally-separated states. Here, we report the first detailed measurements of such states in an electron-antiproton plasma, and the first observations of the separation dynamics in any centrifugally-separated system. While the observed equilibrium states are expected and in agreement with theory, the equilibration time is approximately constant over a wide range of parameters, a surprising and as yet unexplained result. Electron-antiproton plasmas play a crucial role in antihydrogen trapping experiments. Charges in cold, multiple-species, non-neutral plasmas separate radially by mass, forming centrifugally-separated states. Here, we report the first detailed measurements of such states in an electron-antiproton plasma, and the first observations of the separation dynamics in any centrifugally-separated system. While the observed equilibrium states are expected and in agreement with theory, the equilibration time is approximately constant over a wide range of parameters, a surprising and as yet unexplained result. Electron-antiproton plasmas play a crucial role in antihydrogen trapping experiments.

We present the results of an experiment to search for trapped antihydrogen atoms with the ALPHA antihydrogen trap at the CERN Antiproton Decelerator. Sensitive diagnostics of the temperatures, sizes, and densities of the trapped antiproton and positron plasmas have been developed, which in turn permitted development of techniques to precisely and reproducibly control the initial experimental parameters. The use of a position-sensitive annihilation vertex detector, together with the capability of controllably quenching the superconducting magnetic minimum trap, enabled us to carry out a high-sensitivity and low-background search for trapped synthesised antihydrogen atoms. We aim to identify the annihilations of antihydrogen atoms held for at least 130 ms in the trap before being released over ~30 ms. After a three-week experimental run in 2009 involving mixing of 10^7 antiprotons with 1.3 10^9 positrons to produce 6 10^5 antihydrogen atoms, we have identified six antiproton annihilation events that are consistent with the release of trapped antihydrogen. The cosmic ray background, estimated to contribute 0.14 counts, is incompatible with this observation at a significance of 5.6 sigma. Extensive simulations predict that an alternative source of annihilations, the escape of mirror-trapped antiprotons, is highly unlikely, though this possibility has not yet been ruled out experimentally., Comment: 12 pages, 7 figures

We demonstrate controllable excitation of the center-of-mass longitudinal motion of a thermal antiproton plasma using a swept-frequency autoresonant drive. When the plasma is cold, dense, and highly collective in nature, we observe that the entire system behaves as a single-particle nonlinear oscillator, as predicted by a recent theory. In contrast, only a fraction of the antiprotons in a warm plasma can be similarly excited. Antihydrogen was produced and trapped by using this technique to drive antiprotons into a positron plasma, thereby initiating atomic recombination.

This paper reports on a preliminary study of applying a wireless sensor network using DTN (Delay Tolerant Network) to sample the current status of a certain wildlife mammal with the name of White Tail Deer (Odocoileus virginianus) in the WMU Area 47 Ontario, Canada North of Parry Sound district. System architecture is proposed to obtain the highest monitoring range achievable with the least amount of sensors. With this method we can obtain the status of white tail deer, and detect other kinds of animals as well. The current method for surveying the white tail deer needs to be reviewed in order to obtain a most frequent status report of this important animal. By using DTN we expect to obtain disruption support capabilities in our sensor network. Furthermore, we simulated the wildlife habitat monitoring system on the Planet Lab environment. Our future work will develop sensors, deploy them in the field, and create a DTN network for the monitoring of wildlife habitat.

Antimatter was first predicted in 1931, by Dirac. Work with high-energy antiparticles is now commonplace, and anti-electrons are used regularly in the medical technique of positron emission tomography scanning. Antihydrogen, the bound state of an antiproton and a positron, has been produced at low energies at CERN (the European Organization for Nuclear Research) since 2002. Antihydrogen is of interest for use in a precision test of nature's fundamental symmetries. The charge conjugation/parity/time reversal (CPT) theorem, a crucial part of the foundation of the standard model of elementary particles and interactions, demands that hydrogen and antihydrogen have the same spectrum. Given the current experimental precision of measurements on the hydrogen atom (about two parts in 10(14) for the frequency of the 1s-to-2s transition), subjecting antihydrogen to rigorous spectroscopic examination would constitute a compelling, model-independent test of CPT. Antihydrogen could also be used to study the gravitational behaviour of antimatter. However, so far experiments have produced antihydrogen that is not confined, precluding detailed study of its structure. Here we demonstrate trapping of antihydrogen atoms. From the interaction of about 10(7) antiprotons and 7 × 10(8) positrons, we observed 38 annihilation events consistent with the controlled release of trapped antihydrogen from our magnetic trap; the measured background is 1.4 ± 1.4 events. This result opens the door to precision measurements on anti-atoms, which can soon be subjected to the same techniques as developed for hydrogen.

A comparison of the 1S-2S transitions of hydrogen and antihydrogen will yield a stringent test of CPT conservation. Necessarily, the antihydrogen atoms need to be trapped to perform high precision spectroscopy measurements. Therefore, an approximately 0.75 T deep neutral atom trap, equivalent to about 0.5 K for ground state (anti)hydrogen atoms, has been superimposed on a Penning-Malmberg trap in which the anti-atoms are formed. The antihydrogen atoms are produced following a number of steps. A bunch of antiprotons from the CERN Antiproton Decelerator is caught in a Penning-Malmberg trap and subsequently sympathetically cooled and then compressed using rotating wall electric fields. A positron plasma, formed in a separate accumulator, is transported to the main system and also compressed. Antihydrogen atoms are then formed by mixing the antiprotons and positrons. The velocity of the anti-atoms, and their binding energies, will strongly depend on the initial conditions of the constituent particles, for example their temperatures and densities, and on the details of the mixing process. In this paper the complete lifecycle of antihydrogen atoms will be presented, starting with the production of the constituent antiparticles and the description of the manipulations necessary to prepare them appropriately for antihydrogen formation. The latter will also be described, as will the possible fates of the anti-atoms.