1. Determination of daptomycin in human plasma by liquid chromatography-tandem mass spectrometry. Clinical application
- Author
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Nicolas Collet, Danièle Bentué-Ferrer, Olivier Tribut, Eric Bellissant, Matthieu Revest, Marie-Clémence Verdier, Unité Laboratoire CIC, Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM)-Service de pharmacologie biologique et toxicologie [Rennes], CHU Pontchaillou [Rennes]-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de pharmacologie biologique et toxicologie [Rennes], CHU Pontchaillou [Rennes]-Hôpital Pontchaillou, Service des maladies infectieuses et réanimation médicale [Rennes] = Infectious Disease and Intensive Care [Rennes], CHU Pontchaillou [Rennes], Pharmacologie du Sepsis et Choc Septique, Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM)-Service de pharmacologie biologique et toxicologie [Rennes], Hôpital Pontchaillou-CHU Pontchaillou [Rennes]-CHU Pontchaillou [Rennes], Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), and Hôpital Pontchaillou-CHU Pontchaillou [Rennes]
- Subjects
daptomycin ,Clinical Biochemistry ,Bacteremia ,Tandem mass spectrometry ,01 natural sciences ,MESH: Daptomycin ,Drug Stability ,Liquid chromatography–mass spectrometry ,Tandem Mass Spectrometry ,MESH: Drug Monitoring ,liquid chromatography-tandem mass spectrometry ,MESH: Bacteremia ,Chromatography, High Pressure Liquid ,Antibacterial agent ,0303 health sciences ,MESH: Middle Aged ,medicine.diagnostic_test ,Chemistry ,General Medicine ,Middle Aged ,Staphylococcal Infections ,3. Good health ,Anti-Bacterial Agents ,Calibration ,Drug Monitoring ,medicine.drug ,Electrospray ionization ,therapeutic drug monitoring ,MESH: Staphylococcus epidermidis ,MESH: Staphylococcal Infections ,Mass spectrometry ,MESH: Calibration ,03 medical and health sciences ,MESH: Drug Stability ,MESH: Anti-Bacterial Agents ,medicine ,Staphylococcus epidermidis ,Protein precipitation ,Humans ,MESH: Chromatography, High Pressure Liquid ,Chromatography ,MESH: Humans ,030306 microbiology ,010401 analytical chemistry ,Biochemistry (medical) ,MESH: Tandem Mass Spectrometry ,0104 chemical sciences ,Therapeutic drug monitoring ,Daptomycin ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
Background: Daptomycin is a recently developed cyclic lipopeptide antibiotic active against most Gram-positive pathogens including vancomycin-resistant enterococci and methicillin-resistant Staphylococcus aureus. To optimize treatment efficacy and safety, especially in patients undergoing multiple drug regimens and/or co-morbidities, a specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of daptomycin in plasma. Methods: A C18 column was used for separation, with a mobile phase initially consisting of 0.1% formic acid, water, and acetonitrile (ACN) in a linear gradient from 20% to 70%. After protein precipitation with ACN, the clear upper layer was diluted in water:ACN (50:50, v/v) before injection. Detection was performed using an electrospray ionization technique. MS/MS transitions, monitored in the positive ion mode were m/z 811.1 → m/z 313.1 for daptomycin, and m/z 609.4 → m/z 194.9 for reserpine, used as internal standard. Results: Elution of daptomycin and reserpine occurred at 4.5 and 3.9 min, respectively. The method was validated over a range of concentrations from 1 mg/L to 120 mg/L. The assay met recommended acceptance criteria: coefficients of variation were Conclusions: This method appears well-adapted to routine hospital practice for therapeutic drug monitoring of daptomycin considering its time of analysis, range of concentrations measured, precision and accuracy.
- Published
- 2011