The development of a human immunodeficiency virus (HIV) vaccine able to induce neutralizing antibodies against a broad spectrum of primary isolates is complicated by the large diversity of HIV type 1 (HIV-1) strains, the continual mutation of the envelope (Env) glycoprotein in the face of immune selective pressure, and the presence of numerous N-linked glycans that mask polypeptide epitopes (7). Indeed, genetic deletion of N-linked carbohydrate sites can greatly increase the sensitivity of HIV-1 to antibody-mediated neutralization (3, 12, 25, 26, 34, 35). One of the few broadly neutralizing monoclonal antibodies (MAbs) isolated from HIV-1-infected patients, 2G12, circumvents these obstacles by binding to relatively conserved high-mannose-type oligosaccharides exposed on the glycan shield of the gp120 subunit of Env (47, 49, 54). The 2G12 epitope consists of an array of at least three such glycans presented as a dense cluster of terminal mannose sugars (49, 54). Crystal structures of the 2G12 Fab in complex with carbohydrates reveal a specificity toward Manα1,2Man disaccharides, alone or terminally exposed on the D1 and D3 arms of Man9GlcNAc2 (Man9) and Man8GlcNAc2 (Man8) structures, without recognizing other mannose disaccharides, including Manα1,3Man and Manα1,6Man (8, 9). The relatively conserved nature of the 2G12 epitope and the role of N-linked carbohydrates in protecting HIV-1 from antibodies make the glycan shield of Env a viable vaccine target (46, 48). The gp120 subunit of the HIV-1 Env protein contains an average of 25 N-linked glycosylation sites, approximately half of which are composed of high-mannose or hybrid-type glycans (13, 31, 59). To mimic the 2G12 epitope, glycoantigens have been constructed by several laboratories through chemical synthesis of mannose oligosaccharides and chemoenzymatic conjugation to different scaffolds (8, 9, 27, 29, 41, 56). However, to our knowledge, these approaches have yet to elicit antibodies that cross-react with gp120 or neutralize the virus. An alternative approach is to identify and produce other proteins that contain carbohydrate structures similar to those comprising the 2G12 epitope on HIV-1 Env. Analysis of the Saccharomyces cerevisiae genome reveals the presence of numerous proteins that contain a large number of potential N-linked glycosylation sites, making yeast a possible source of proteins with closely arrayed N-linked glycans with the potential to cross-react with the 2G12 antibody. However, while essentially identical high-mannose core structures are added to the N-linked glycosylation sites on both yeast and mammalian cell proteins in the endoplasmic reticulum (ER) (4, 14, 18, 23), subsequent carbohydrate processing pathways in the Golgi apparatus diverge significantly. In yeast cells, numerous mannose residues are added to the core structure in the Golgi apparatus, forming polymannose-type glycans (14). Over a dozen proteins in the Golgi apparatus of S. cerevisiae are involved in processing N-glycans (20), three of which are vital for the modification of the core Man8 structure that is exported from the ER (Fig. (Fig.1).1). In the cis-Golgi, Och1p initiates the first mannose residue necessary for the extended α1,6-linked mannose branch, a key component of polymannose-type glycans (30). Deletion of the Och1 gene alone leads to the lack of the outer chain, resulting in a majority of Man9 and Man10 structures, which represent core Man8 capped at the D1 and/or D3 arm with α1,3-linked mannose residues (40). In the trans-Golgi, Mnn1p is the sole α1,3-mannosytransferase responsible for adding these α1,3-mannose caps to the core glycans (11, 39, 58), while Mnn4p is a positive regulator involved in adding phosphomannose residues to both the core and outer chain (43, 44). FIG. 1. N-linked glycosylation pathway in wild-type and Δoch1 Δmnn1 Δmnn4 S. cerevisiae. In the ER, after en bloc transfer of Glc3Man9GlcNAc2 to nascent polyproteins, the three glucose residues are cleaved. In wild-type yeast, N-linked ... In this study, we sought to mimic the 2G12 epitope on the HIV-1 Env protein by producing a yeast strain that would express exclusively unprocessed, high-mannose glycans. We achieved this by generating a triple mutant of S. cerevisiae, a Δoch1 Δmnn1 Δmnn4 mutant (that we named TM [for triple mutant]) that produced almost homogenous Man8 glycans. The MAb 2G12 bound efficiently to the TM mutant, but not to the wild-type S. cerevisiae (that we named WT [for wild type]). At least four heavily glycosylated S. cerevisiae proteins supported 2G12 binding, with each of these possessing numerous N-linked glycosylation sites at a high density. Importantly, immune sera raised with whole cells of this mutant yeast, but not WT, cross-reacted in a carbohydrate-dependent manner with a broad array of mammalian cell-expressed Env glycoproteins from HIV-1 and simian immunodeficiency virus (SIV) strains, suggesting that genetically modified yeast proteins may serve as molecular scaffolds that recapitulate carbohydrate-dependent epitopes on the surface of the HIV-1 Env protein.