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An infectious West Nile Virus that expresses a GFP reporter gene
- Source :
- Virology. 334:28-40
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- West Nile virus is a mosquito-borne, neurotropic flavivirus that causes encephalitis in humans and animals. Since being introduced into the Western hemisphere in 1999, WNV has spread rapidly across North America, identifying this virus as an important emerging pathogen. In this study, we developed a DNA-launched infectious molecular clone of WNV that encodes a GFP reporter gene. Transfection of cells with the plasmid encoding this recombinant virus (pWNII-GFP) resulted in the production of infectious WNV capable of expressing GFP at high levels shortly after infection of a variety of cell types, including primary neurons and dendritic cells. Infection of cells with WNII-GFP virus was productive, and could be inhibited with both monoclonal antibodies and interferon-β, highlighting the potential of this system in the development and characterization of novel inhibitors and therapeutics for WNV infection. As expected, insertion of the reporter gene into the viral genome was associated with a reduced rate of viral replication, providing the selective pressure for the development of variants that no longer encoded the full-length reporter gene cassette. We anticipate this DNA-based, infectious WNV reporter virus will allow novel approaches for the study of WNV infection and its inhibition both in vitro and in vivo.
- Subjects :
- viruses
Green Fluorescent Proteins
Gene Expression
Biology
Antibodies, Viral
Transfection
Virus Replication
Recombinant virus
Virus
Cell Line
Green fluorescent protein
Plasmid
Genes, Reporter
Molecular clone
Cricetinae
Virology
Animals
Humans
West Nile Virus
Reporter gene
Flavivirus
Antibodies, Monoclonal
virus diseases
biology.organism_classification
Recombinant Proteins
Kinetics
Viral replication
Interferon Type I
HeLa Cells
Plasmids
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 334
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....87dd8ff8d2b6c7c216ab1987383daeee