Search

Endophytic fungi is an important source for the discovery of bioactive natural compounds. A chemical investigation of the ethyl acetate extract of the endophytic fungus Schizophyllum sp. HM230 derived from stems of the herb Vincetoxicum mongolicum Maxim led to isolation of five alkaloids, including two new compounds, schizophyllins M (1) and N (2), along with three known ones (3–5). The planar structures of two new compounds were elucidated by extensive spectroscopic methods including MS, 1D and 2D NMR. Their absolute configurations were determined by Mosher’s method and comparison of the ECD data. All the isolates were evaluated for their cytotoxicity and antioxidant activities. Compounds 1–4 showed middle cytotoxicity against MCF-7 cells with IC50 values range of 68.1 ∼ 87.32 μM. Compounds 1–5 displayed obvious antioxidant activity with the IC50 values range of 0.86 ∼ 5.78 mg/mL.

The minimum variation timescale (MVT) of soft gamma-ray repeaters can be an important probe to estimate the emission region in pulsar-like models, as well as the Lorentz factor and radius of the possible relativistic jet in gamma-ray burst (GRB)-like models, thus revealing their progenitors and physical mechanisms. In this work, we systematically study the MVTs of hundreds of X-ray bursts (XRBs) from SGR J1935+2154 observed by {\it Insight}-HXMT, GECAM and Fermi/GBM from July 2014 to Jan 2022 through the Bayesian Block algorithm. We find that the MVTs peak at $\sim$ 2 ms, corresponding to a light travel time size of about 600 km, which supports the magnetospheric origin in pulsar-like models. The shock radius and the Lorentz factor of the jet are also constrained in GRB-like models. Interestingly, the MVT of the XRB associated with FRB 200428 is $\sim$ 70 ms, which is longer than that of most bursts and implies its special radiation mechanism. Besides, the median of MVTs is 7 ms, shorter than the median MVTs of 40 ms and 480 ms for short GRBs or long GRBs, respectively. However, the MVT is independent of duration, similar to GRBs. Finally, we investigate the energy dependence of MVT and suggest that there is a marginal evidence for a power-law relationship like GRBs but the rate of variation is at least about an order of magnitude smaller. These features may provide an approach to identify bursts with a magnetar origin., accepted for publication in ApJS

Xiaotian Shi, Shan Wang, Jieqiong Hu, Fei Chen, Heng Zhang, Yifan Yang, Xv Li, Qing Ma Department of Geriatrics, Beijing Friendship Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Qing Ma, Email maqing3@163.comObjective: Determine the relationship of renal function with frailty using different formulas for estimated glomerular filtration rate (eGFR).Methods: Individuals who were 60-years-old or more (n=507) were recruited from August 2020 to June 2021, and the FRAIL scale was used to classify them as non-frail or frail. The three equations used to compute the eGFR were based on serum creatinine (eGFRcr), cystatin C (eGFRcys), or SCr+CysC (eGFRcr-cys). Renal function was classified using eGFR and defined as normal (≥ 90 mL/min/1.73m2), mild damage (59– 89 mL/min/1.73m2), or moderate damage (≤ 60 mL/min/1.73m2). The relationship of frailty with renal function was analyzed. A subset of participants (n=358) was used to analyze changes in eGFR from 1 January 2012 to 31 December 2021 according to frailty and using the different eGFR equations.Results: There were significant differences between the eGFRcr-cys and eGFRcr values in the frail group (P< 0.05), but not the non-frail group; however, the differences between the eGFRcr-cys and eGFRcys values were significant in the frail and non-frail groups (P< 0.001). Based on each eGFR equation, the prevalence of frailty increased as eGFR decreased (P< 0.001), but there was no significant relationship after adjusting for age or the age-adjusted Charlson co-morbidity index. There were temporal declines in eGFR in all three frailty groups (robust, pre-frail, and frail), especially in the frail group (2.226 mL/min/1.73m2 per year; P< 0.001).Conclusion: For older individuals who are frail, the eGFRcr value may not provide accurate estimates of renal function. Frailty is associated with a rapid decline in kidney function.Keywords: elderly, frailty, kidney function, serum creatinine, cystatin C

Hao Chen,1,2,&ast; Qing Ma,3,&ast; Bo Du,1 Yan Huang,1 Shi-Guang Zhu,1 Sheng-Li Li,4 De-Qin Geng,1 Xing-Shun Xu2,5,6 1Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221000, People’s Republic of China; 2Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou, 215000, People’s Republic of China; 3Department of Anesthesiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221000, People’s Republic of China; 4Department of Medical Records and Statistics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221000, People’s Republic of China; 5Institute of Neuroscience, Soochow University, Suzhou, 215000, People’s Republic of China; 6Jiangsu Key Laboratory of Neuropsychiatric Diseases, Soochow University, Suzhou, 215000, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: De-Qin Geng, Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221000, People’s Republic of China, Tel +86 18052268197, Email gengdeqingdq@outlook.com Xing-Shun Xu, Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou, 215000, People’s Republic of China, Tel +86 13915411326, Email xuxingshunxxs@126.comObjective: The present study aims to analysis the mental health of high-risk health care workers (HHCWs) and low-risk HCWs (LHCWs) who were respectively exposed to COVID-19 wards and non-COVID-19 wards by following up on mental disorders in HCWs in China for 6 months.Methods: A multi-psychological assessment questionnaire was used to follow up on the psychological status of HCWs in the Affiliated Hospital of Xuzhou Medical University in Xuzhou City (a non-core epidemic area) at 6 months after the first evaluation conducted during the COVID-19 epidemic. Based on the risk of exposure to COVID-19 patients, the HCWs were divided into two groups: high-risk HCWs, who worked in COVID-19 wards, and low-risk HCWs, who worked in non-COVID-19 wards.Results: A total of 198 HCWs participated in the study, and 168 questionnaires were selected for evaluation. Among them, 93 (55.4%) were in the HHCW group and 75 (44.5%) were in the LHCW group. Significant differences were observed in salary, profession, and altruistic behavior between the two groups (P < 0.05). There were no significant differences in the anxiety, depression, insomnia, or posttraumatic stress disorder (PTSD) scores between the two groups. Logistic regression revealed that work stress was a major joint risk factor for mental disorders in HCWs. Among all the HCWs, a total of 58 voluntarily participated in psychotherapy; the analysis showed a significant decrease in anxiety, depression, PTSD, work stress, and work risk after attending psychotherapy. There were also significant differences in positive and negative coping styles before and after psychotherapy.Conclusion: In the present follow-up, work stress was the major contributing factor to mental disorders in HCWs. Psychotherapy is helpful in terms of stress management and should be provided to first-line COVID-19 HCWs.Keywords: COVID-19, anxiety, depression, posttraumatic stress disorder, insomnia, work stress

Due to the non-linear ionizing and heating processes, the 21-cm signals from epoch of reionization (EoR) are expected to have strong non-Gaussian fluctuations. In this paper, we use the semi-numerical simulations to study the non-Gaussian statistics i.e. skew spectrum and smoothed skewness of the 21-cm signals from EoR. We find the 21-cm skew spectrum and smoothed skewness have similar evolution features with the 21-cm bispectrum. All of them are sensitive to the EoR models, while not too much to the cosmic volume applied. With the SKA1-low telescope as reference, we find both the skew spectrum and smoothed skewness have much higher S/N ratios than the 21-cm bispectrum., 7 pages, 5 figures, mnras accepted

ObjectiveTo identify author collaborations and impact; participating countries, institutions, and journals; evaluate the knowledge base; and analyze research hotspots and frontiers in teaching reforms in physiology.MethodsArticles and reviews related to teaching reforms in physiology published between January 1, 2012, and December 31, 2021, were obtained from the Web of Science Core Collection. Two Scientometric software applications (CiteSpace 5.7 and VOSviewer 1.6.15) were used to perform bibliometric and knowledge-map analysis, generate network maps, and identify research trends and top keywords, authors, co-cited authors, institutions, countries, journals, and references.ResultsThe search identified a total of 2,882 papers in 466 academic journals by 13,895 authors from 4,072 organizations in 67 countries/regions. Physiology teaching reform-related publications increased rapidly over time. Arango-Lasprilla and Rivera published the most papers, while Moseley had the most co-citations. Active collaborations among physiology researchers were noted. Advances in Physiology Education published the most papers on physiology teaching reforms and was also the top co-cited journal in the Medicine/Medical/Clinical, Psychology/Education/Health, and Neurology/Sports/Ophthalmology fields. The United States and University of California published the most physiology teaching publications in the search criteria. Ten references (research articles and reviews) on mechanisms and diseases were identified as the knowledge base. The mainstream research directions were education, Alzheimer’s disease, performance, physiology, and risk factors. Mental health and emotion regulation are increasing in significance and may become new hotspots. The research trend to move from the field of pain pathogenesis to the field of neuropsychiatry has become increasingly clear. This tendency away from peripheral system-based disorders to central system-based orders is inextricably linked to further developments in physiological understanding of the brain.ConclusionThis study analyzed the research hot spots and frontiers of teaching reforms on in physiology using bibliometric and visual methods. Based on the results, rehabilitation, neurosciences, and infectious disease are hot topics in physiology. In particular, the pathogenesis of neurological diseases, treatment strategies, and technology updates have gradually become research hotspots. We predict that this trend is closely related to the implementation of brain research programs in various countries. These findings provide helpful references for scholars focusing on physiology education.

BackgroundNon-intubated video-assisted thoracoscopic surgery (NIVATS) has been increasingly applied worldwide owing to its benefits of enhanced recovery after surgery (ERAS). Anesthetic management for patients with asthma should focus on minimizing airway stimulation.Case descriptionA 23-year-old male patient with a history of asthma was diagnosed with left-sided spontaneous pneumothorax. The patient then underwent left-sided NIVATS bullectomy under general anesthesia with preserved spontaneous breathing. Left thoracic paravertebral nerve block (TPVB) with an injection of 0.375% ropivacaine (30 ml) was performed in the 6th paravertebral space under ultrasound guidance. Anesthesia induction commenced until the cold sensation in the surgical area had disappeared. General anesthesia was induced by midazolam, penehyclidine hydrochloride, esketamine, and propofol and then maintained using propofol and esketamine. Surgery commenced after the patient was positioned in the right lateral recumbency. The collapse of the left lung was satisfactory, and the operative field was ensured after artificial pneumothorax. The surgical procedure was uneventful, intraoperative arterial blood gases were within normal ranges, and vital signs were stable. The patient awakened rapidly without any adverse reactions at the end of the surgery and was then transferred to the ward. During the postoperative follow-up, the patient experienced mild pain 48 h after surgery. The patient was discharged from the hospital 2 days postoperatively and developed no nausea, vomiting, or any other complications.ConclusionThe present case suggests the feasibility of TPVB in combination with non-opioid anesthetics to provide high-quality anesthesia in patients undergoing NIVATS bullectomy.

BackgroundBovine viral diarrhea is one of the diseases that cause huge economic losses in animal husbandry. Many countries or regions have successively introduced eradication plans, but BVDV still has a high prevalence in the world. This meta-analysis aims to investigate the prevalence and risk factors of BVDV in the world in recent 10 years, and is expected to provide some reference and theoretical basis for BVDV control plans in different regions.MethodRelevant articles published from 2010 to 2021 were mainly retrieved from NCBI, ScienceDirect, Chongqing VIP, Chinese web of knowledge (CNKI), web of science and Wanfang databases.Results128 data were used to analyze the prevalence of BVDV from 2010 to 2021. BVDV antigen prevalence rate is 15.74% (95% CI: 11.35–20.68), antibody prevalence rate is 42.77% (95% CI: 37.01–48.63). In the two databases of antigen and antibody, regions, sampling time, samples, detection methods, species, health status, age, sex, breeding mode, and seasonal subgroups were discussed and analyzed, respectively. In the antigen database, the prevalence of dairy cows in the breed subgroup, ELISA in the detection method subgroup, ear tissue in the sample subgroup, and extensive breeding in the breeding mode were the lowest, with significant differences. In the antibody database, the prevalence rate of dairy cows in the breed subgroup and intensive farming was the highest, with a significant difference. The subgroups in the remaining two databases were not significantly different.ConclusionThis meta-analysis determined the prevalence of BVDV in global cattle herds from 2010 to 2021. The prevalence of BVDV varies from region to region, and the situation is still not optimistic. In daily feeding, we should pay attention to the rigorous and comprehensive management to minimize the spread of virus. The government should enforce BVDV prevention and control, implement control or eradication policies according to local conditions, and adjust the policies in time.

Additional file 1: Supplementary Methods 1. MRI data Acquisition and Processing in UKB. SupplementaryMethods 2. MRI data Acquisition and Processing in ABCD. SupplementaryMethods 3. Included measures. Fig. S1. Polygenic risk scores. Fig.S2. Associations between AD PRS (constructed using GWAS data reported by Kunkle et al.) and cortical macrostructural MRI metrics in ABCD. Fig.S3. Associations between AD PRS (constructed using GWAS data reported by Kunkle et al.) and volume of subcortical structures in ABCD. Fig.S4. Associations between AD PRS (constructed using GWAS data reported by Kunkle et al.) and white matter microstructural MRI metrics inABCD. Fig. S5. The correlation between PRSs constructed by different GWAS data (Schwartzentruber et al. 2021 and Kunkle et al. 2019) in ABCD sample. Fig. S6. Associations between AD PRS (excluding APOE along with adjust APOE genotype)and cortical macrostructural MRI metrics. Fig. S7. Associations betweenAD PRS (excluding APOE along with adjust APOE genotype) and volume ofsubcortical structures. Fig. S8. Associations between AD PRS (excluding APOE along with adjust APOE genotype)and white matter microstructural MRI metrics. Fig. S9. Trends in thecorrelation between association results (adjust 40PCs or 10PCs) of PRSs withbrain structures in UKB samples. Fig. S10. Trends in the correlationbetween association results (adjust birth weight and gestational age or not) ofPRSs with brain structures in ABCD samples. Fig. S11. Trends in thecorrelation between association results (outliers simply removed or winsorized)of PRSs with brain structures in ABCD samples.

Additional file 2: Table S1.Overview of RNA sequencing data. Table S2. Sequencing production statistics. Table S3. Summary of sequence annotation. Table S4. DEGs related to MYBs and HSFs in roots of Z. xanthoxylum under heat treatments. Fold change equals to log2 (the RPKM value of a gene undertreatment / the RPKM value of a gene under control condition) and indicates thetranscript abundance change of each DEGs. Homologous gene and homologous species were obtained by NCBI blast according to the sequence corresponding to the gene ID. Table S5. DEGs relatedto AP2/ERFs in roots of Z. xanthoxylum identified only at 40°C for 0.5 h. Foldchange equals to log2 (40°C-0.5 h RPKM / 25°C-0.5 h RPKM) and indicates thetranscript abundance change of each DEGs. Homologous gene and homologous species were obtained by NCBI blast according to the sequence corresponding tothe gene ID. Table S6. DEGs relatedto WRKYs in roots of Z. xanthoxylum identified only at 40°C for 0.5 h. Foldchange equals to log2 (40°C-0.5 h RPKM / 25°C-0.5 h RPKM) and indicates thetranscript abundance change of each DEGs. Homologous gene and homologousspecies were obtained by NCBI blast according to the sequence corresponding tothe gene ID. Table S7. DEGs related to HSFs in roots of Z. xanthoxylum identified only at 40°C for 0.5 h. Foldchange equals to log2 (40°C-0.5 h RPKM / 25°C-0.5 h RPKM) and indicates thetranscript abundance change of each DEGs. Homologous gene and homologous species were obtained by NCBI blast according to the sequence corresponding tothe gene ID. Table S8. DEGs relatedto HSPs in roots of Z. xanthoxylum identified under heat treatments. Foldchange equals to log2 (the RPKM value of a gene under treatment / the RPKM value of a gene under control condition) and indicates the transcript abundancechange of each DEGs. Homologous gene and homologous species were obtained by NCBI blast according to the sequence corresponding to the gene ID. Table S9. DEGs related to HSPs in roots of Z. xanthoxylum identified only at 40°C for 6 h. Fold change equals to log2 (40°C-6 h RPKM / 25°C-6 h RPKM) and indicates the transcript abundance change of each DEGs. Homologous gene and homologous species were obtained by NCBI blast according to the sequence corresponding to the gene ID. Table S10. DEGs related to PSI inleaves of Z. xanthoxylum identified only at 40°C for 0.5 h. Fold change equalsto log2 (40°C-0.5 h RPKM / 25°C-0.5 h RPKM) and indicates the transcript abundance change of each DEGs. Homologous gene and homologous species were obtained by NCBI blast according to the sequence corresponding to the gene ID. Table S11. The sequences used for RT-qPCR validation.

Additional file 1: Figure S1.The water content (a), osmotic potential (b) and relative membrane permeability(c) of leaves in Z. xanthoxylum under control and heat treatments for 10 days(6 h/d). Values in (a-c) are mean ± SE (n = 5). Asterisks indicate significantdifferences in comparison with control (25°C) (* P < 0.05, ** P < 0.01,*** P < 0.001, Student’s t-test). Figure S2. Functional annotation of the assembled transcriptome. (a) Lengthdistribution of all assembled unigenes; (b) Map of GO functional categories; (c) Map of KOG function classifications. Figure S3. Number of DEGs in leaves (a) and roots (b) of Z. xanthoxylum under heat treatments. Figure S4. Enriched GO terms for DEGs in leaves of Z. xanthoxylum at 40°C (a, c) and 45°C (b, d) for 0.5 h (a, b) and 6 h (c, d). Figure S5. Enriched GO terms for DEGs in roots of Z. xanthoxylum at 40°C (a, c) and 45°C(b, d) for 0.5 h (a, b) and 6 h (c, d). Figure S6. Enriched KEGG terms for DEGs in leaves of Z. xanthoxylum at 40°C (a, c) and 45°C (b, d) for 0.5 h (a, b) and 6 h (c, d). Figure S7. Enriched KEGG terms for DEGs in roots of Z. xanthoxylumat 40°C (a, c) and 45°C (c, d) for 0.5 h (a, b) and 6 h (b, d). Figure S8. Correlation analysis for 20 selected DEGs betweenRNA-seq and RT-qPCR results in leaves (a-d) and roots (e-h) of Z. xanthoxylum under heat treatments.

Supplementary Material 1

BackgroundGastroenteropancreatic neuroendocrine carcinoma (GEP-NEC) is a group of rare but highly aggressive malignancies. The standard chemotherapy regimens composed of etoposide and cisplatin/carboplatin (EP/EC) are of limited efficacy. This prospective, multicenter, phase II study is conducted to explore the effectiveness and safety of first-line anti-PD-1 antibody (camrelizumab) combined with chemotherapy in advanced GEP-NEC patients.MethodsPatients with unresectable or metastatic GEP-NEC will receive camrelizumab combined with standard first-line chemotherapy every 3 weeks (camrelizumab 200 mg, administered intravenously on day 1; etoposide 100 mg/m2, administered intravenously on days 1–3; cisplatin 75 mg/m2, administered intravenously on day 1 or carboplatin area under the curve 5 mg/ml per min, administered intravenously on day 1). All patients were naïve to systemic therapy in the advanced setting. The primary endpoint is a 6-month progression-free survival (PFS) rate. The secondary endpoints are objective response rate, PFS, overall survival and adverse reactions.DiscussionThis is the first study to investigate the therapeutic potential of camrelizumab plus chemotherapy for advanced GEP-NEC. It is expected that this trial will propose a new and effective treatment strategy for GEP-NEC in the first-line setting.Clinical Trial RegistrationThis trial is registered at the Chinese Clinical Trial Registry http://www.chictr.org.cn, identifier ChiCTR2100047314.Date of RegistrationJune 12, 2021.

Maya R Chilbert,1,2 Dylan VanDuyn,3 Sara Salah,1 Collin M Clark,1,2 Qing Ma1 1Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, NY, USA; 2Department of Pharmacy, Buffalo General Medical Center, Buffalo, NY, USA; 3Department of Pharmacy, Buffalo Veterans Affairs Medical Center, Buffalo, NY, USACorrespondence: Maya R Chilbert, Department of Pharmacy Practice, University at Buffalo School of Pharmacy and Pharmaceutical Sciences, 202 Pharmacy Building, Buffalo, NY, USA, Tel +1 716 829-5172, Fax +1 716-829-6093, Email mayahols@buffalo.eduAbstract: Cardiovascular disease is one of the leading causes of death around the world with various efforts being made to reduce risk in patients through preventive measures. One major method for prevention has been managing cholesterol, particularly low-density lipoprotein to decrease atherosclerotic plaque burden, potentially decreasing future cardiac complications. Statins have been the gold standard therapy for hypercholesterolemia treatment due to their ease of dosing, limited drug interactions, and favorable safety profile. Unfortunately, statin therapy alone is not always effective enough to adequately control a patient’s elevated lipid levels and combination therapy may be warranted. Ezetimibe is commonly added to regimens to help augment cholesterol lowering by inhibiting the absorption of cholesterol. The recent approval of a combination tablet of high-intensity rosuvastatin and ezetimibe has introduced a potentially more beneficial option for cholesterol management in addition to the only available combination of moderate intensity simvastatin and ezetimibe. We aimed to identify potential beneficial effects of ezetimibe by comparing its use in combination with high-intensity rosuvastatin compared to a statin therapy alone or in combination with moderate intensity simvastatin through a literature review. The current evidence indicated that combination therapy outperformed statin monotherapy in reduction of low-density lipoprotein cholesterol and patients were more likely to achieve their target low-density lipoprotein cholesterol goal level. This suggests rosuvastatin/ezetimibe combination holds a potential place in therapy for patients requiring a more aggressive reduction in cholesterol to help prevent atherosclerotic disease.Keywords: hypercholesterolemia, hyperlipidemia, cardiac events, lipid biomarkers, atherosclerosis

Objective To evaluate the clinical efficacy of percutaneous transluminal angioplasty (PTA) combined with stent implantation in the treatment of transplant renal artery stenosis (TRAS) after renal transplantation. Methods Clinical data of 21 patients with TRAS after renal transplantation undergoing PTA combined with stent implantation were retrospectively analyzed. The incidence of TRAS in renal transplant recipients was summarized. The changes of relevant indexes in patients with TRAS were statistically compared before and after interventional treatment. Clinical prognosis of patients with TRAS was evaluated. Results The incidence of TRAS in renal transplant recipients was 4.1%(21/507). TRAS was diagnosed at postoperative 5 (4, 7) months, and 67% (14/21) of patients developed TRAS within postoperative 6 months. Compared with the values before interventional therapy, the serum creatinine level, systolic and diastolic blood pressure and peak flow velocity of transplant renal artery of patients with TRAS were significantly decreased, and the estimated glomerular filtration rate (eGFR) and interlobar arterial resistance index were significantly increased at 1 week and 1 month after interventional therapy (all P < 0.05). During postoperative follow-up after PTA combined with stent implantation, 1 patient suffered re-stenosis of the transplant renal artery, which was improved after simple balloon dilatation. One patient developed pseudoaneurysm formation at the puncture site of the right femoral artery. One patient presented with renal atrophy and loss of function due to atresia of the transplant renal artery. All the remaining 18 patients were well recovered after surgery. Conclusions PTA combined with stent implantation is the optimal treatment of TRAS after renal transplantation, which can significantly improve the function of transplant kidney and considerably prolong the survival time of transplant kidney.

Thyroid storm is usually due to a severe exacerbation of a preexisting thyrotoxicosis, which later leads to decompensation in different organ systems. Thyroid storm with malignant arrhythmia is rare, but the mortality rate is high. Malignant arrhythmia has usually occurred in some patients with hypokalemia or ischemic heart disease. The presentation of these arrhythmias in the initial phase of the disease is much less common, and only a few isolated cases are described in the scientific literature. This paper analyzes and summarizes the clinical characteristics of this disease through literature reviews, for clinicians early detection and diagnosis of this disease. We report a 51-year-old woman with diarrhoea, fever, thyroid storm, ventricular fibrillation and no potential heart disease. The case report is discussed using relevant data from literature. A total of 9 patients were reported in the literature, totalling 10 cases with ours, including 7 cases of ventricular fibrillation and 3 cases of ventricular tachycardia. Most of the treatments were antithyroid drugs, hormones and beta-receptor blockers, and 3 death cases. Patients with thyroid crisis need to be closely monitored for malignant arrhythmias. Early diagnosis and treatment may save lives.

Aiming at the problem of poor robustness of traditional user behavior pattern mining analysis method, a cloud computing-based intelligent home user behavior pattern mining analysis method is designed. Intelligent household IoT from using cloud computing method and data mining the user behavior patterns, establish a two-layer neural network level of data is divided into 2 kinds, the user behavior mode by setting the input weight vector calculation after classifying data correlation between user behavior model, using Apriori algorithm, input minimum support and minimum confidence, on the basis of analyzing the correlation between data, and establish the user behavior mode decision tree, on the basis of complete analysis of cloud computing smart home user behavior patterns mining method design. Through the comparison experiment with the traditional method, it is concluded that the designed mining analysis method based on cloud computing has higher robustness, the proposed cloud computing-based intelligent home user behavior pattern mining method has good application space.

Highly regular TiO2 nanotube arrays (TNTs) with interstitial nitrogen as the dominant point defect have been successfully prepared by annealing the as-anodized TNTs in N2 in a temperature range of 400–650 °C. Using analysis methods that include field emission scanning electron microscopy, X-ray diffraction patterns, and Raman spectroscopy, the thermal stability of TNTs annealed in N2 was found to be higher than that of TNTs annealed in O2. The interstitial nitrogen is considered as the dominant point defect in TNTs annealed in N2, which results in the notably improved thermal stability of the highly ordered TNTs. However, the content of hydroxyl oxygen on the surface of TNTs is significantly influenced by annealing temperature, which is closely related to photocatalytic activity. Optimized photocatalytic property of photodegradation of MO with a pseudo-first-order reaction constant k value of (5.065 ± 0.139) × 10–2 h−1 is achieved for TNTs annealed in N2 at 600 °C. These findings serve to provide simple and versatile guidelines to fabricate highly ordered TNTs with controllable point defects, enhanced thermal stability, and a high level of photocatalytic activity.

Aiming to solve the problem of environmental information being difficult to characterize when an intelligent service is used, knowledge graphs are used to express environmental information when performing intelligent services. Here, we specially design a kind of knowledge graph for environment expression referred to as a robot knowledge graph (R-KG). The main work of a R-KG is to integrate the diverse semantic information in the environment and pay attention to the relationship at the instance level. Also, through the efficient knowledge organization of a R-KG, robots can fully understand the environment. The R-KG firstly integrates knowledge from different sources to form a unified and standardized representation of a knowledge graph. Then, the deep logical relationship hidden in the knowledge graph is explored. To this end, a knowledge reasoning model based on a Markov logic network is proposed to realize the self-developmental ability of the knowledge graph and to further enrich it. Finally, as the strength of environment expression directly affects the efficiency of robots performing services, in order to verify the efficiency of the R-KG, it is used here as the semantic map that can be directly used by a robot for performing intelligent services. The final results prove that the R-KG can effectively express environmental information.

Additional file 4: Table S2. Statistics of lncRNA sequencing from the six libraries.

Additional file 6: Table S4. GO terms enriched in the biological process category for the 953 common targets.

Additional file 1: Table S1. Demographic characteristics of adolescents atninth-grade born with different gestational weeks from the Danish cohort study.Table S2. Group difference for gestational age and cognitive measures at 9-10 years. Table S3. Validation of group difference for gestational age andcognitive measures at 9-10 years. Table S4. Group difference for gestational age and cognitive measures at 11-12years. Table S5. Groupdifference for the association between gestational age and brain volume in adolescents at 9-10 years. Table S6.Mediation result for mediation by each volumetric region of the association between gestational age and cognitive measures in adolescents at 9-10 years. Table S7. Longitudinal group-by-time interaction for brain volume in adolescents from 9-10 years to 11-12 years. Table S8. Group difference for theassociation between gestational age and brain volume when excluding adolescentswith extreme birth weight at 9-10 years. Table S9. Group difference for the association between gestationalage and brain volume at 9-10 years when excluding adolescents with Caesareian birth. Table S10. Group-by-income interaction for brain volumes in adolescents at 9-10 years. Fig. S1. The distribution of gestational weeks at both baseline and 2-years follow-up neuroimaging analyses.Fig. S2. Differences of cognitive performance between any two gestational weeks. Fig. S3. Brain regions with lower volumes for different gestational age. Fig. S4. Positive correlation between gestational age and whole brain volumes at baseline. Fig. S5.Subcortical/non-neocortical volume change from 9-10 years to 11-12 years. Fig. S6. Proportion of adolescents taking the final examination according to gestational weeks from the Danish cohort study.

Additional file 1. Describes the Behavioral testing, RT-QPCR, Western blot analysis, Immunohistochemistry experimental procedure, and Statistical analysis in detail.

The current action recognition method has good effect when applied to static recognition, but, when applied to dynamic action sequence recognition, the temporal and spatial feature segmentation is too dependent on sample template, resulting in low recognition accuracy. To address the inadequacies of standard movement detection techniques in the application of comparable domains, a deep learning algorithm is utilised to recognise Tai Chi Chuan motions. For Tai Chi Chuan movement human body skeleton framework, add image depth parameter is added, and OpenPose model is utilised to estimate joint point coordinates. The ST-GCN deep learning model was created to recognise Tai Chi Chuan motions by extracting movement features from the spatiotemporal trajectory of human joints during Tai Chi Chuan movements. Instance test results show that rate of using the deep learning algorithm of gesture recognition is 89.22%, with significantly lower error detection rate, which is good for Tai chi chuan movement recognition effect.

To solve the ill-posed problem of hyperspectral image super-resolution (HSISR), an usually method is to use the prior information of the hyperspectral images (HSIs) as a regularization term to constrain the objective function. Model-based methods using hand-crafted priors cannot fully characterize the properties of HSIs. Learning-based methods usually use a convolutional neural network (CNN) to learn the implicit priors of HSIs. However, the learning ability of CNN is limited, it only considers the spatial characteristics of the HSIs and ignores the spectral characteristics, and convolution is not effective for long-range dependency modeling. There is still a lot of room for improvement. In this paper, we propose a novel HSISR method that uses Transformer instead of CNN to learn the prior of HSIs. Specifically, we first use the proximal gradient algorithm to solve the HSISR model, and then use an unfolding network to simulate the iterative solution processes. The self-attention layer of Transformer makes it have the ability of spatial global interaction. In addition, we add 3D-CNN behind the Transformer layers to better explore the spatio-spectral correlation of HSIs. Both quantitative and visual results on two widely used HSI datasets and the real-world dataset demonstrate that the proposed method achieves a considerable gain compared to all the mainstream algorithms including the most competitive conventional methods and the recently proposed deep learning-based methods., 10 pages, 5 figures

In order to satisfy the robotic personalized service requirements that can select exclusive items to perform inference and planning according to different service individuals, the service robots need to have the ability to independently obtain the ownership relationship between humans and their carrying items. In this work, we present a novel semantic learning strategy for item ownership. Firstly, a human-carrying-items detection network based on human posture estimation and object detection model is used. Then, the transferred convolutional neural network is used to extract the characteristics of the objects and the back-end classifier to recognize the object instance. At the same time, the face detection and recognition model are used to identify the service individual. Finally, on the basis of the former two, the active learning of ownership items is completed. The experimental results show that the proposed ownership semantic learning strategy can determine the ownership relationship of private goods accurately and efficiently. The solution of this problem can improve the intelligence level of robot life service.

Fe-based amorphous alloy has excellent soft magnetic properties; traditionally, Fe-based amorphous alloy such as soft magnetic devices was fabricated by insulation enveloping and suppression molding methods. In this process, the aging of organic envelope materials and the crystallization of Fe-based amorphous alloy were usually occurring, accompanying with low magnetic induction and poor mechanical properties. The direct ink writing (DIW) technique can make complex-shaped parts and needs no heating treatment after forming, which can avoid the effect of traditional molding process. In the present study, varying mass fraction FeSiB/EP composite parts were prepared by the DIW technique with the Fe-based amorphous alloy powder and epoxy resin, in which microscopic morphology, magnetic properties, and mechanical properties of FeSiB/EP soft magnetic composites were studied. The results indicate that the slurry with iron powder mass fraction of 92.3, 92.6, and 92.8 wt% has good printing performance and self-support ability, which is suitable for DIW. The density of the printed parts is about 4.317, 4.449, and 4.537 g/cm3, which is almost similar with the iron powder. The tensile strength and elongation of printing parts are significantly improved compared with the pure epoxy resin. From the photos of microscopic morphology of printing parts, it can be seen that FeSiB powders are evenly dispersed in EP, no pores, and defects, with the proportion increasing of powders; the insulation coating thickness decreases; and the magnetic performance improves. The optimal sample is 92.8 wt% FeSiB/EP, in which saturation magnetic induction strength is 137.9759 emu/g and coercivity is 4.6523 A/m.

Arbuscular mycorrhizal fungi regulated the distribution of nitrogen in the leaves, thereby facilitating the adaptation of the endangered plant Torreya jackii to a low-nitrogen environment. Rhizophagus irregularis was inoculated into sterilized soil to investigate its impact on the distribution ratio of leaf nitrogen in cell wall proteins, cell membrane proteins, water-soluble proteins, and photosynthetic systems which includes the carboxylation system (PC), energy metabolism (PB), and light-harvesting system in the endangered species Torreya jackii. The results showed that R. irregularis reduced the specific leaf weight and the distribution ratio of nitrogen in cell wall proteins in the leaves of T. jackii, whereas it enhanced the distribution ratio of nitrogen in cell membrane proteins and water-soluble proteins. R. irregularis enabled more nitrogen uptake for growth by decreasing the distribution of nitrogen to the structural substances. At low-nitrogen levels, inoculation with R. irregularis improved the plant height (18.78 ~ 36.04%), shoot dry weight (50.53 ~ 64.33%), total dry weight (42.86 ~ 52.82%), maximal net photosynthetic rate (Pmax) (16.83 ~ 20.11%), photosynthetic nitrogen use efficiency (PNUE) (40.01 ~ 43.14%), PC (33.56 ~ 38.59%) and PB (29.08 ~ 34.02%). However, it did not substantially affect the leaf nitrogen content per unit area or the leaf nitrogen content per unit mass. Moreover, Pmax exhibited a significant positive correlation with PC and PB, and all three parameters showed a significant positive correlation with the PNUE, thereby revealing that R. irregularis increased the photosynthetic capacity and PNUE of T. jackii through boosting PC and PB.

Mitochondrial epigenetics is rising as intriguing notion for its potential involvement in aging and diseases, while the details remain largely unexplored. Here it is shown that among the 13 mitochondrial DNA (mtDNA) encoded genes, NADH‐dehydrogenase 6 (ND6) transcript is primarily decreased in obese and type 2 diabetes populations, which negatively correlates with its distinctive hypermethylation. Hepatic mtDNA sequencing in mice unveils that ND6 presents the highest methylation level, which dramatically increases under diabetic condition due to enhanced mitochondrial translocation of DNA methyltransferase 1 (DNMT1) promoted by free fatty acid through adenosine 5’‐monophosphate (AMP)‐activated protein kinase (AMPK) activation. Hepatic knockdown of ND6 or overexpression of Dnmt1 similarly impairs mitochondrial function and induces systemic insulin resistance both in vivo and in vitro. Genetic or chemical targeting hepatic DNMT1 shows significant benefits against insulin resistance associated metabolic disorders. These findings highlight the pivotal role of ND6 epigenetic network in regulating mitochondrial function and onset of insulin resistance, shedding light on potential preventive and therapeutic strategies of insulin resistance and related metabolic disorders from a perspective of mitochondrial epigenetics., Metabolic disorders emerge as one of the biggest public health challenges with distinctive feature of elevated free fatty acid, which can promote mitochondrial translocation of DNA methyltransferase 1 in liver, resulting in hypermethylation of NADHdehydrogenase 6 (ND6) on mitochondrial DNA. Such molecular change suppresses ND6 expression and induces hepatic mitochondrial dysfunction, eventually provokes systemic insulin resistance.

Polymer derived ceramics (PDCs) process has been one of the predominant fabrication technologies for ceramic materials. As a desirable raw material for the preparation of ceramics with high performance-to-cost ratio, polysiloxane (PSO) derived SiOC ceramics have been well developed. The SiOC ceramics derived from hetero element-modified PSO are the research hotspot of PDCs technology in recent years because the thermal stability can be enhanced and the functional properties can be expanded by the incorporation of hetero element. In this paper, microstructure of SiOC ceramics was introduced first, and then the current status of hetero element-modified PSO derived SiOC ceramics was reviewed from the viewpoints of improving thermal stability and expanding functional properties, respectively. In subsequent study, two key problems which should be paid much attention are to elucidate the operation mechanism of hetero element based on the evolution of atom local chemical environment and to enhance the application of modified SiOC ceramics.

Synthetic X-ray images are simulated X-ray images projected from CT data. High-quality synthetic X-ray images can facilitate various applications such as surgical image guidance systems and VR training simulations. However, it is difficult to produce high-quality arbitrary view synthetic X-ray images in real-time due to different CT slice thickness, high computational cost, and the complexity of algorithms. Our goal is to generate high-resolution synthetic X-ray images in real-time by upsampling low-resolution images with deep learning-based super-resolution methods. Reference-based Super Resolution (RefSR) has been well studied in recent years and has shown higher performance than traditional Single Image Super-Resolution (SISR). It can produce fine details by utilizing the reference image but still inevitably generates some artifacts and noise. In this paper, we introduce frequency domain loss as a constraint to further improve the quality of the RefSR results with fine details and without obvious artifacts. To the best of our knowledge, this is the first paper utilizing the frequency domain for the loss functions in the field of super-resolution. We achieved good results in evaluating our method on both synthetic and real X-ray image datasets.

Two cardenolide glycosides, corotoxigenin 3-O-[β-D-glucopyranosyl-(1→4)-6-deoxy-β-D-glucopyranoside] ( 1 ) and coroglaucigenin 3-O-[β-D-glucopyranosyl-(1→4)-6-deoxy-β-D-glucopyranoside] ( 2 ), were isolated from the seed fairs of Asclepias curassavica L.. The structures of 1 − 2 were determined based on a combination of the analysis of their MS, NMR spectroscopic data and acid hydrolysis. The inhibitory effects of compounds 1 and 2 on human colorectal carcinoma cells (HCT116), non-small cell lung carcinoma cells (A549) and hepatic cancer cells (SMMC-7721) were evaluated. The results showed that both compounds 1 and 2 could significantly inhibit the viability, proliferation, and migration of A549, HCT116 and SMMC-7721 cells, suggesting that compounds 1 and 2 could be applied in the treatment of lung, colon and liver cancers in clinical practice. This study may not only provide a scientific basis for clarifying the active ingredients in A. curassavica L., but also help to understand its antitumor activity, which could promote the application of A. curassavica L. in clinical treatment of various cancers.

Objective: To explore the effects of off-pump coronary artery bypass grafting (OPC) on clinical efficacy, cardiac function and the occurrence of major adverse cardiovascular events (MACE) in patients with coronary heart disease. Methods: According to different surgical methods, 93 patients with coronary heart disease who were hospitalized and treated in our hospital were collapsed into the off-pump coronary artery bypass grafting group (OPC group) and the extracorporeal circulation coronary artery bypass grafting group (PC group). The perioperative indexes, cardiac function indexes, postoperative recovery, quality of life, the incidence of MACE and adverse reactions and the survival rate of patients in PC group and OPC group were analyzed and compared. Results: Compared with the PC group, the operation time and blood transfusion volume of OPC group were both largely decreased (P0.05). The cardiac function index of OPC group was much higher than that of PC group (P0.05). Conclusion: Compared with PC, OPC has a significantly better therapeutic effect on patients with coronary heart disease, which can significantly improve the perioperative indices and cardiac function, and enhance the quality of life of patients.

Additional file 1. Percentage of reads number with different lengths at three reproductive stages of sheep.

Additional file 1. SPIRIT 2013 Checklist: Recommended items to address in a clinical trial protocol and related documents*.

Additional file 5. The expression level of target genes for DE miRNAs between anestrus and breeding season in different reproductive stages.

Additional file 7. Highly expressed genes during anestrus or breeding season in pineal gland of sheep.

Additional file 9 Stem-loop primers employed for RT-PCR of miRNAs and primers employed for Q-PCR of AANAT and miRNAs.

Additional file 8. Predicted miRNAs potentially targeting genes in ribosome pathway.

Additional file 10. Mature sequences of miRNAs and inserted sequences in the pcDNA6.2-GW/EmGFP-miR vector.

Additional file 3. The signaling pathways which the stage-specific expressed miRNAs were predominantly enriched in.

Additional file 9 Stem-loop primers employed for RT-PCR of miRNAs and primers employed for Q-PCR of AANAT and miRNAs.