197 results on '"Lingyan Chen"'
Search Results
2. Rehmannioside D mitigates disease progression in rats with experimental-induced diminished ovarian reserve via Forkhead Box O1/KLOTHO axis
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Yan Liang, Huimin Wang, Jin Chen, Lingyan Chen, and Xiaoyong Chen
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Pharmacology ,Physiology - Published
- 2023
3. Ki-67 Index Provides Long-Term Survival Information for Early-Stage HER2-Low-Positive Breast Cancer: A Single-Institute Retrospective Analysis
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Wei-Xiang Qi, Lingyan Chen, Lu Cao, Cheng Xu, Gang Cai, and Jiayi Chen
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Article Subject ,Oncology - Abstract
Aim. It has been reported that more than half of breast cancer (BC) could be identified as HER2-low-positive, which might be a distinct subtype. But the results are controversial. We aim to compare the survival outcomes between HER2-low-positive and HER2-0 BC with Asian women based on HR status or Ki-67 index. Methods. Between January 2009 and December 2017, HER2-nonamplified BC in our single institute was identified. Patients were classified as HER2-low and HER2-0 cohort. Clinical characteristics were compared between these two groups and survival outcomes were calculated by the Kaplan–Meier method. We also performed subgroup analysis according to Ki-67 index and hormone-receptor (HR) status. Results. Of the 2,230 included patients, 536 presented with HER2-0, and 1,694 with HER2-low positive. After a median follow-up of 85 months (range: 1–152 months), the 8-year OS, BCSS, and RFS of the overall cohort were 91%, 95%, and 89%, respectively. In comparison with the HER2-0 cohort, majority of HER2-low-expression BC concurrently presented with HR positive (82.3% vs. 69%, P < 0.001 ). There was no significant survival difference between the two groups in terms of OS, BCSS, and RFS (all p > 0.05 ). We then performed subgroup analysis according to HR status and Ki-67 index ( p > 0.05 ), while OS ( p = 0.026 ) and BCSS ( p = 0.052 ) of HER2-0 BC with high Ki-67 index were significantly poorer than that of HER2-low positive with high Ki-67, but not for RFS ( p = 0.17 ). Conclusion. Among early stage HER2-nonamplified BC, no significant survival difference could be found between HER2-low positive and HER2-0 cohort regardless of HR status. Survival outcomes of HER2-low positive with high Ki-67 seem to be poorer than that of HER2-0 tumors with high Ki-67 index.
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- 2022
4. Hyperuricemia Associated with Low Skeletal Muscle in the Middle-Aged and Elderly Population in China
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Lingyan Chen, Li Wu, Qian Li, Yu Hu, Hui Ma, Huandong Lin, and Xin Gao
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Male ,China ,Endocrinology, Diabetes and Metabolism ,Cholesterol, HDL ,Hyperuricemia ,General Medicine ,Middle Aged ,Body Mass Index ,Uric Acid ,Cross-Sectional Studies ,Endocrinology ,Internal Medicine ,Humans ,Female ,Muscle, Skeletal ,Aged - Abstract
Background Previous studies have presented inconsistent results on the relationship between serum uric acid and skeletal muscle mass (SMM). We aimed to explore whether a higher serum uric acid level was associated with low SMM in the Chinese population. Methods We performed a cross-sectional analysis of 6595 subjects aged 45 years or older. They were tested for fasting blood glucose, total cholesterol, triglycerides, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, uric acid, blood urea nitrogen, creatinine, and estimated glomerular filtration rate. SMM was accessed by dual-energy x-ray absorptiometry using two approaches: weight-adjusted appendicular skeletal muscle mass (ASM)% and ASM/BMI (body mass index (kg/m2)). Low SMM was defined as a cut-off point of ASM/BMI Results Compared with their normal group, patients with hyperuricemia had lower ASM% (29.33±2.33 vs 30.03±2.34 for males and 24.71±1.99 vs 25.19±2.07 for females, P Conclusions In a middle-aged and elderly Chinese population, hyperuricemia is independently and positively associated with low SMM and can vary by age and gender.
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- 2022
5. Liposomal Doxorubicin: the Sphingomyelin/Cholesterol System Significantly Enhances the Antitumor Efficacy of Doxorubicin
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Xianmin Meng, Hongxia Zhang, Lingyan Chen, Mingqi Wang, Kaituo Zhang, Xinrong Liu, Yihui Deng, and Yanzhi Song
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Ecology ,Drug Discovery ,Pharmaceutical Science ,General Medicine ,Aquatic Science ,Agronomy and Crop Science ,Ecology, Evolution, Behavior and Systematics - Published
- 2023
6. Evaluation and Optimization of Cultural Perception of Coastal Greenway Landscape Based on Structural Equation Model
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Yili Yang, Yuxing Chen, Yueyan Liu, Tianyou He, and Lingyan Chen
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Health, Toxicology and Mutagenesis ,island-type greenway ,structural equation model ,Public Health, Environmental and Occupational Health ,regional culture ,landscape evaluation ,cultural perception - Abstract
The island-type greenway should emphasize the role of maintaining and promoting the island cultural landscape as it serves the function of a general greenway green infrastructure while also having a unique landscape appearance. The northern greenway of Pingtan is used as an example in the paper to illustrate how regional culture is perceived. The first part of the analysis looks at how demographic factors affect the quality of cultural perception. The study reveals that: from a gender perspective, women are more likely than men to perceive regional culture; from an age perspective, people between the ages of 18 and 40 are more likely to perceive regional culture; older people and children are less likely to perceive regional culture; and from a level of education perspective, the higher the education, the stronger the perception. The relationship between tourists’ perceived quality, cognitive image, perceived value, satisfaction, and loyalty to the cultural expression of the greenway landscape is then analyzed by building a structural equation model. According to the findings, visitors’ perceptions of the island’s cultural quality have a positive impact on their cognitive images and perceptions of value, while their satisfaction with the cultural expressions along the coastal greenway has a positive impact on their loyalty.
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- 2023
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7. Mapping of bamboo forest bright and shadow areas using optical and SAR satellite data in Google Earth Engine
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Songyang Xiang, Zhanghua Xu, Wanling Shen, Lingyan Chen, Zhenbang Hao, Lin Wang, Zhicai Liu, Zenglu Li, Xiaoyu Guo, and Huafeng Zhang
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Geography, Planning and Development ,Water Science and Technology - Abstract
Bamboo groves predominantly thrive in tropical or subtropical regions. Assessing the efficacy of remote sensing data of various types in extracting bamboo forest information from bright and shadow areas is a critical issue for achieving precise identification of bamboo forests in complex terrain. In this study, 34 features were obtained from Sentinel-1 SAR and Sentinel-2 optical images using the Google Earth Engine platform. The normalized shaded vegetation index (NSVI) was then employed to segment the bright and shadow woodlands. Different features from diverse data sources were evaluated to extract bamboo forest information in the bright and shadow areas, then use the random forest (RF) classification algorithm to extract bamboo forest. The results showed that (1) the red-edge and short-wave infrared bands of Sentinel-2 optical images and their corresponding vegetation indices are significant in bamboo forest information extraction. (2) The dissimilarity and homogeneity of Sentinel-2 texture features in the bright area and dissimilarity in the shadow area, the Sentinel-1 backscatter features in the bright area and the VV and VH in the bright area and VV-VH in the shadow area have some variability between bamboo and nonbamboo forests, which can be used as effective features for bamboo forest extraction. (3) The combination of spectral, texture and backscatter features yields the highest overall classification accuracy and Kappa coefficient, at 87.96% and 0.7435, respectively. This study has the potential for remote sensing refinement of bamboo forest identification in complex terrain areas by utilizing subregion classification methods combined with optical and radar image features.
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- 2023
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8. Association of osteoporosis and skeletal muscle loss with serum type I collagen carboxyl-terminal peptide β glypeptide: A cross-sectional study in elder Chinese population
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Lingyan Chen, Jiayu Wu, Weiying Ren, Xi Li, Man Luo, and Yu Hu
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General Medicine - Abstract
Type I collagen carboxyl-terminal peptide β (β-CTX) increases in osteoporosis. The study aimed to explore the relationship between serum β-CTX and the risk of osteoporosis as well as sarcopenia in Chinese elderly inpatients. Around 228 patients whose age >65 years were recruited in this cross-sectional study. Dual-energy X-ray scanning was used to access skeletal muscle and bone mass. Serum concentration of β-CTX as well as the prevalence of osteoporosis were significantly higher in low skeletal muscle index (SMI) group than that in the normal SMI group (P < 0.05). Serum β-CTX levels negatively correlated with SMI and bone mass (P < 0.05). Total muscle mass, appendicular skeletal muscle mass, SMI, total bone mass, and bone mass at various sites including the limbs, spine, and pelvis decreased significantly, and the prevalence of low SMI increased with the increase of the quartiles of β-CTX. Higher serum β-CTX had an increased risk of low SMI and osteoporosis (P < 0.05). Summarily, with increasing serum β-CTX levels, both muscle and bone mass decreased in Chinese elderly inpatients. Serum β-CTX was positively associated with the risk of not only osteoporosis but also skeletal muscle loss.
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- 2023
9. Indicative value of pathological classification of duodenal papillary adenomas in clinical diagnosis and treatment
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Ying Lv, Pin Wang, Jun Chen, Li Zhao, Lingyan Chen, Yingjia Zhuang, Lei Wang, and Xiaoping Zou
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Adenoma ,Ampulla of Vater ,Treatment Outcome ,Common Bile Duct Neoplasms ,Humans ,Margins of Excision ,Surgery ,Neoplasm Recurrence, Local ,Pancreaticoduodenectomy ,Retrospective Studies - Abstract
The relationship between the pathological classification and recurrence of duodenal papillary adenomas (DPAs) has not been elucidated. We studied the clinicopathological characteristics of DPAs with different pathological types and conducted long-term follow-up to explore its prognosis and identify methods for appropriate clinical management of DPAs.In total, 95 DPA cases confirmed by postoperative pathology were enrolled, of which 58 underwent endoscopic papillectomy (EP) and 37 underwent pancreatoduodenectomy (PD). The cases were classified into three anatomical and two histomorphological types according to the histopathology and location of endoscopic features. We analyzed the clinicopathological characteristics of DPAs with different pathological types and investigated the factors associated with recurrence in the EP subgroup.Although EP was associated with fewer adverse events, the complete resection rate was significantly lower (72.4% vs. 100.0%, p 0.001) and the recurrence rate significantly higher than with PD (16.3% vs. 0.0%, p 0.001). Among eight EP cases with recurrence, six had intra-DPA (75%). A positive resection margin (HR 23.67, 95% CI 6.42-87.27; p 0.001) and MUC2-negative status (HR 3.47, 95% CI 1.16-10.40; p = 0.026) were independent risk factors for recurrence after EP.We identified different pathological types within DPAs, which presented varying clinicopathological features. The majority of peri-DPAs and mixed-DPAs were of the intestinal type histologically and EP is the primary recommendation. However, intra-DPA was mainly of the pancreaticobiliary type, which tends to get positive resection margins; thus, surgical resection is more suitable.
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- 2022
10. NAFLD‐related gene polymorphisms and all‐cause and cause‐specific mortality in an Asian population: the Shanghai Changfeng Study
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Mingfeng Xia, Shuai Ma, Qingxia Huang, Hailuan Zeng, Jieyu Ge, Wenjie Xu, Qi Wu, Li Wu, Xiaoming Li, Hui Ma, Lingyan Chen, Qian Li, Qiqige Aleteng, Yu Hu, Wanyuan He, Baishen Pan, Huandong Lin, Yan Zheng, Sijia Wang, Huiru Tang, and Xin Gao
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Adult ,China ,Genotype ,Hepatology ,Gastroenterology ,Membrane Proteins ,Lipase ,Polymorphism, Single Nucleotide ,Liver ,Cardiovascular Diseases ,Non-alcoholic Fatty Liver Disease ,Cause of Death ,Humans ,Genetic Predisposition to Disease ,Pharmacology (medical) ,Obesity ,Genome-Wide Association Study - Abstract
The PNPLA3 and TM6SF2 gene variants have been found to cause NAFLD with a favourable cardiovascular risk profile.To investigate the effects of the NAFLD risk alleles on the all-cause and cause-specific mortality in 5581 Chinese adults.The genome-wide genotypes were detected using a genotyping array and serum lipoprotein profiles were examined using 1H NMR platform. Liver fat content (LFC) was measured using a quantitative ultrasound method. The vital status was determined using official registration data.Genome-wide association analysis showed that a series of variants in PNPLA3 were associated with LFC, including rs738409 CG variant (P = 8.6 × 10The PNPLA3 rs738409 CG variant and its combination with TM6SF2 rs58542926 CT and MBOAT7 rs641738 CT variants increase liver-specific mortality but reduce cardiovascular mortality in overweight/obese Chinese.
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- 2022
11. Tanshinone IIa Induces Autophagy and Apoptosis via PI3K/Akt/mTOR Axis in Acute Promyelocytic Leukemia NB4 Cells
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Ruibai Li, Li Hou, Mengdie Nan, Yiming Pan, Yu Liu, and Lingyan Chen
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Acute promyelocytic leukemia ,Article Subject ,medicine.diagnostic_test ,Chemistry ,Autophagy ,medicine.disease ,Flow cytometry ,Other systems of medicine ,Complementary and alternative medicine ,Downregulation and upregulation ,Apoptosis ,medicine ,Cancer research ,Viability assay ,Protein kinase B ,RZ201-999 ,PI3K/AKT/mTOR pathway ,Research Article - Abstract
Tanshinone IIa (TanIIa), an ingredient of Radix Salviae Miltiorrhizae, has an anticancer effect on various solid tumors with high efficiency and low toxicity. Nonetheless, the underlying role of TanIIa in acute promyelocytic leukemia (APL) remains unclear. Here, we revealed that TanIIa drastically inhibited NB4 cell viability with an IC50 value of 31.25 μmol/L. Using flow cytometry apoptosis assay, we identified that TanIIa dose-dependently exacerbated NB4 cell apoptosis. Mechanistically, TanIIa upregulated apoptotic factor levels, namely, cleaved-caspase 9, cleaved-caspase 3, and cleaved-PARP-1. Moreover, we noticed that TanIIa dose-dependently suppressed the PI3K/Akt/mTOR axis. This axis not only functions as an essential antiapoptotic modulator but also serves as a suppressant regulator of autophagy. Correspondingly, we detected the levels of autophagic marker, namely, LC3B, which were increased after the TanIIa treatment. Furthermore, the autophagy inhibitor Baf-A1 could effectively reverse the TanIIa-induced apoptosis, manifesting that TanIIa eliminated NB4 cells in an autophagy-dependent manner. In conclusion, tanshinone IIa exerts anti-APL effects through triggering autophagy and apoptosis in NB4 cells.
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- 2021
12. Genome-Wide Identification of MIKCc-Type MADS-Box Family Gene and Floral Organ Transcriptome Characterization in Ma Bamboo (Dendrocalamus latiflorus Munro)
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Deming Yang, Jing Yang, Jiayi Wan, Yanping Xu, Lei Li, Jundong Rong, Lingyan Chen, Tianyou He, and Yushan Zheng
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bamboo ,floral organ ,MADS-box ,Dendrocalamus latiflorus Munro ,pollen abortion ,Genetics ,Genetics (clinical) - Abstract
Most bamboos die after flowering, and the molecular mechanisms responsible for flowering is poorly understood. The MIKCc-type MADS-box family gene is involved in the flowering process. To explore the mechanism of the MIKCc-type MADS-box gene and phytohormone regulation in the flowering of Dendrocalamus latiflorus Munro (D. latiflorus), characterized by extremely rapid growth and widely cultivated woody bamboo, we initially did a genome-wide analysis of the MIKCc-type MADS-box gene in D. latiflorus. In the meantime, transcriptome analysis was performed using the floral organs. A total of 170 MIKCc-Type MADS-Box genes were identified and divided into 15 categories. The cis-acting element analysis in promoters regions revealed that MIKC-type MADS-box family genes were associated with hormones, including auxin, abscisic acid (ABA), gibberellin (GA) and jasmonic acid (JA), which was found at 79, 476, 96, 486 sites and cover 61, 103, 73, 128 genes. Genome synteny analysis showed subgenome AA and BB were better than CC and obtained 49, 40, 39 synteny genes compared with Oryza sativa (O. sativa). In transcriptome analysis of floral organs, the enriched pathway from DEGs included circadian, vernalization and gibberellin pathways associated with the flowering process. We found that the jasmonic acid synthesis gene is highly expressed in the pistil, which may be the cause of Ma bamboo pollen abortion. The expression profile showed that most MIKC-type MADS-box genes exhibited high expression in flower organs. The consequences of this study will provide insight into the irregular flowering and low pollen counts of Ma bamboo.
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- 2022
- Full Text
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13. Sex disparity of cerebral white matter hyperintensity in the hypertensive elderly: The Shanghai Changfeng study
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Liangqi Wang, Huandong Lin, Zehua Zhao, Lingyan Chen, Li Wu, Ting Liu, Jing Li, Chu‐Chung Huang, Yifeng Peng, Chun‐Yi Zac Lo, and Xin Gao
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Neurology ,Radiological and Ultrasound Technology ,Radiology, Nuclear Medicine and imaging ,Neurology (clinical) ,Anatomy - Abstract
White matter hyperintensity (WMH) is associated with vascular hemodynamic alterations and reflects white matter injury. To date, the sex difference of tract-specific WMH and the relationship between high blood pressure (BP) and tract-specific WMH remain unclear. We recruited 515 subjects from the Shanghai Changfeng study (range 53-89 years, mean age 67.33 years). Systolic and diastolic blood pressure (SBP and DBP) were collected and used to calculate pulse pressure (PP). Magnetic resonance T1 and T2 FLAIR images were acquired to measure WMH and calculate WMH index. The ANCOVA test was performed to test the difference between sexes, and the linear regression model was used to examine the associations between BP and WMH index. Men showed higher WMH index than women in all white matter tracts (p .001, respectively) except for the bilateral superior longitudinal fasciculus (SLF) and its left temporal part (tSLF). High SBP and PP was associated with a lower WMH index on the left corticospinal tract (CST), SLF, tSLF and right cingulum in hippocampus (p ≤ .001, respectively) in women, while high DBP was associated with a higher WMH index on the bilateral CST (left p .001; right p = .001), left inferior longitudinal fasciculus (p .001) and inferior fronto-occipital fasciculus (p = .002) in men. Men tend to have more WMH compared to women. A high SBP/PP relates to a lower WMH burden in women. This suggests that women could benefit from higher blood pressure in older age.
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- 2022
14. Mild-to-Moderate Kidney Dysfunction and Cardiovascular Disease: Observational and Mendelian Randomization Analyses
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Liam Gaziano, Luanluan Sun, Matthew Arnold, Steven Bell, Kelly Cho, Stephen K. Kaptoge, Rebecca J. Song, Stephen Burgess, Daniel C. Posner, Katja Mosconi, Cassianne Robinson-Cohen, Amy M. Mason, Thomas R. Bolton, Ran Tao, Elias Allara, Petra Schubert, Lingyan Chen, James R. Staley, Natalie Staplin, Servet Altay, Pilar Amiano, Volker Arndt, Johan Ärnlöv, Elizabeth L.M. Barr, Cecilia Björkelund, Jolanda M.A. Boer, Hermann Brenner, Edoardo Casiglia, Paolo Chiodini, Jackie A. Cooper, Josef Coresh, Mary Cushman, Rachel Dankner, Karina W. Davidson, Renate T. de Jongh, Chiara Donfrancesco, Gunnar Engström, Heinz Freisling, Agustín Gómez de la Cámara, Vilmundur Gudnason, Graeme J. Hankey, Per-Olof Hansson, Alicia K. Heath, Ewout J. Hoorn, Hironori Imano, Simerjot K. Jassal, Rudolf Kaaks, Verena Katzke, Jussi Kauhanen, Stefan Kiechl, Wolfgang Koenig, Richard A. Kronmal, Cecilie Kyrø, Deborah A. Lawlor, Börje Ljungberg, Conor MacDonald, Giovanna Masala, Christa Meisinger, Olle Melander, Conchi Moreno Iribas, Toshiharu Ninomiya, Dorothea Nitsch, Børge G. Nordestgaard, Charlotte Onland-Moret, Luigi Palmieri, Dafina Petrova, Jose Ramón Quirós Garcia, Annika Rosengren, Carlotta Sacerdote, Masaru Sakurai, Carmen Santiuste, Matthias B. Schulze, Sabina Sieri, Johan Sundström, Valérie Tikhonoff, Anne Tjønneland, Tammy Tong, Rosario Tumino, Ioanna Tzoulaki, Yvonne T. van der Schouw, W.M. Monique Verschuren, Henry Völzke, Robert B. Wallace, S. Goya Wannamethee, Elisabete Weiderpass, Peter Willeit, Mark Woodward, Kazumasa Yamagishi, Raul Zamora-Ros, Elvis A. Akwo, Saiju Pyarajan, David R. Gagnon, Philip S. Tsao, Sumitra Muralidhar, Todd L. Edwards, Scott M. Damrauer, Jacob Joseph, Lisa Pennells, Peter W.F. Wilson, Seamus Harrison, Thomas A. Gaziano, Michael Inouye, Colin Baigent, Juan P. Casas, Claudia Langenberg, Nick Wareham, Elio Riboli, J.Michael Gaziano, John Danesh, Adriana M. Hung, Adam S. Butterworth, Angela M. Wood, Emanuele Di Angelantonio, Anna Koettgen, Jonathan Shaw, Robert Atkins, Paul Zimmet, Peter Whincup, Johann Willeit, Christoph Leitner, Anne Tybjaerg-Hansen, Peter Schnohr, Shoaib Afzal, David Lora Pablos, Cristina Martin Arriscado, Carmen Romero Ferreiro, Hannah Stocker, Ben Schöttker, Bernd Holleczek, Angela Chetrit, Lennart Welin, Kurt Svärdsudd, Lauren Lissner, Dominique Hange, Kirsten Mehlig, Dorothea Nagel, Paul E. Norman, Osvaldo Almeida, Leon Flicker, Jun Hata, Takanori Honda, Yoshihiko Furuta, Hiroyasu Iso, Akihiko Kitamura, Isao Muraki, Jukka T. Salonen, Tomi-Pekka Tuomainen, E. M. van Zutphen, N. M. van Schoor, Cinzia Lo Noce, Richard Kronmal, Georg Lappas, Peter M. Nilsson, Bo Hedblad, Jonathan Shaffer, Joseph Schwartz, Daichi Shimbo, Shinichi Sato, Mina Hayama-Terada, Simerjot Jassal, Thor Aspelund, Bolli Thorsson, Gunnar Sigurdsson, Layal Chaker, Kamran M. Ikram, Maryam Kavousi, Hugh Tunstall-Pedoe, Günay Can, Hüsniye Yüksel, Uğur Özkan, Hideaki Nakagawa, Yuko Morikawa, Masao Ishizaki, Edith Feskens, Johanna M Geleijnse, Daan Kromhout, Internal Medicine, Neurology, Epidemiology, Bell, Steven [0000-0001-6774-3149], Posner, Daniel C [0000-0002-3056-6924], Mason, Amy M [0000-0002-8019-0777], Allara, Elias [0000-0002-1634-8330], Staplin, Natalie [0000-0003-4482-4418], Arndt, Volker [0000-0001-9320-8684], Ärnlöv, Johan [0000-0002-6933-4637], Barr, Elizabeth LM [0000-0003-4284-1716], Boer, Jolanda MA [0000-0002-9714-4304], Brenner, Hermann [0000-0002-6129-1572], Casiglia, Edoardo [0000-0002-0003-3289], Chiodini, Paolo [0000-0003-0139-2264], Coresh, Josef [0000-0002-4598-0669], Cushman, Mary [0000-0002-7871-6143], Davidson, Karina W [0000-0002-9162-477X], de Jongh, Renate T [0000-0001-8414-3938], Engström, Gunnar [0000-0002-8618-9152], de la Cámara, Agustín Gómez [0000-0001-6827-6319], Gudnason, Vilmundur [0000-0001-5696-0084], Hankey, Graeme J [0000-0002-6044-7328], Hansson, Per-Olof [0000-0001-6323-0506], Heath, Alicia K [0000-0001-6517-1300], Hoorn, Ewout J [0000-0002-8738-3571], Imano, Hironori [0000-0002-6661-4254], Katzke, Verena [0000-0002-6509-6555], Kiechl, Stefan [0000-0002-9836-2514], Koenig, Wolfgang [0000-0002-2064-9603], Kronmal, Richard A [0000-0002-9897-7076], Kyrø, Cecilie [0000-0002-9083-8960], Ljungberg, Börje [0000-0002-4121-3753], MacDonald, Conor [0000-0002-4989-803X], Masala, Giovanna [0000-0002-5758-9069], Ninomiya, Toshiharu [0000-0003-1345-9032], Nordestgaard, Børge G [0000-0002-1954-7220], Onland-Moret, Charlotte [0000-0002-2360-913X], Palmieri, Luigi [0000-0002-4298-2642], Rosengren, Annika [0000-0002-5409-6605], Schulze, Matthias B [0000-0002-0830-5277], Sieri, Sabina [0000-0001-5201-172X], Sundström, Johan [0000-0003-2247-8454], Tikhonoff, Valérie [0000-0001-7846-0101], Tong, Tammy [0000-0002-0284-8959], Tzoulaki, Ioanna [0000-0002-4275-9328], van der Schouw, Yvonne T [0000-0002-4605-435X], Wannamethee, S Goya [0000-0001-9484-9977], Weiderpass, Elisabete [0000-0003-2237-0128], Willeit, Peter [0000-0002-1866-7159], Woodward, Mark [0000-0001-9800-5296], Yamagishi, Kazumasa [0000-0003-3301-5519], Zamora-Ros, Raul [0000-0002-6236-6804], Gagnon, David R [0000-0002-6367-3179], Tsao, Philip S [0000-0001-7274-9318], Edwards, Todd L [0000-0003-4318-6119], Damrauer, Scott M [0000-0001-8009-1632], Joseph, Jacob [0000-0002-7279-4896], Pennells, Lisa [0000-0002-8594-3061], Gaziano, Thomas A [0000-0002-5985-345X], Langenberg, Claudia [0000-0002-5017-7344], Wareham, Nick [0000-0003-1422-2993], Hung, Adriana M [0000-0002-3203-1608], Butterworth, Adam S [0000-0002-6915-9015], Di Angelantonio, Emanuele [0000-0001-8776-6719], Apollo - University of Cambridge Repository, Gaziano, Liam, Sun, Luanluan, Arnold, Matthew, Bell, Steven, Cho, Kelly, Kaptoge, Stephen K, Song, Rebecca J, Burgess, Stephen, Posner, Daniel C, Mosconi, Katja, Robinson-Cohen, Cassianne, Mason, Amy M, Bolton, Thomas R, Tao, Ran, Allara, Elia, Schubert, Petra, Chen, Lingyan, Staley, James R, Staplin, Natalie, Altay, Servet, Amiano, Pilar, Arndt, Volker, Ärnlöv, Johan, Barr, Elizabeth L M, Björkelund, Cecilia, Boer, Jolanda M A, Brenner, Hermann, Casiglia, Edoardo, Chiodini, Paolo, Cooper, Jackie A, Coresh, Josef, Cushman, Mary, Dankner, Rachel, Davidson, Karina W, de Jongh, Renate T, Donfrancesco, Chiara, Engström, Gunnar, Freisling, Heinz, de la Cámara, Agustín Gómez, Gudnason, Vilmundur, Hankey, Graeme J, Hansson, Per-Olof, Heath, Alicia K, Hoorn, Ewout J, Imano, Hironori, Jassal, Simerjot K, Kaaks, Rudolf, Katzke, Verena, Kauhanen, Jussi, Kiechl, Stefan, Koenig, Wolfgang, Kronmal, Richard A, Kyrø, Cecilie, Lawlor, Deborah A, Ljungberg, Börje, Macdonald, Conor, Masala, Giovanna, Meisinger, Christa, Melander, Olle, Moreno Iribas, Conchi, Ninomiya, Toshiharu, Nitsch, Dorothea, Nordestgaard, Børge G, Onland-Moret, Charlotte, Palmieri, Luigi, Petrova, Dafina, Garcia, Jose Ramón Quiró, Rosengren, Annika, Sacerdote, Carlotta, Sakurai, Masaru, Santiuste, Carmen, Schulze, Matthias B, Sieri, Sabina, Sundström, Johan, Tikhonoff, Valérie, Tjønneland, Anne, Tong, Tammy, Tumino, Rosario, Tzoulaki, Ioanna, van der Schouw, Yvonne T, Monique Verschuren, W M, Völzke, Henry, Wallace, Robert B, Wannamethee, S Goya, Weiderpass, Elisabete, Willeit, Peter, Woodward, Mark, Yamagishi, Kazumasa, Zamora-Ros, Raul, Akwo, Elvis A, Pyarajan, Saiju, Gagnon, David R, Tsao, Philip S, Muralidhar, Sumitra, Edwards, Todd L, Damrauer, Scott M, Joseph, Jacob, Pennells, Lisa, Wilson, Peter W F, Harrison, Seamu, Gaziano, Thomas A, Inouye, Michael, Baigent, Colin, Casas, Juan P, Langenberg, Claudia, Wareham, Nick, Riboli, Elio, Gaziano, J Michael, Danesh, John, Hung, Adriana M, Butterworth, Adam S, Wood, Angela M, Di Angelantonio, Emanuele, Internal medicine, AMS - Ageing & Vitality, AMS - Musculoskeletal Health, Amsterdam Gastroenterology Endocrinology Metabolism, Epidemiology and Data Science, APH - Aging & Later Life, and APH - Personalized Medicine
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kidney disease ,General Practice ,Emerging Risk Factors Collaboration/EPIC-CVD/Million Veteran Program ,Coronary Disease ,coronary disease ,Kidney ,Malalties coronàries ,1117 Public Health and Health Services ,Coronary diseases ,SDG 3 - Good Health and Well-being ,cardiovascular disease ,Risk Factors ,Physiology (medical) ,Diabetes Mellitus ,Humans ,Cardiac and Cardiovascular Systems ,Prospective Studies ,1102 Cardiorespiratory Medicine and Haematology ,Kardiologi ,Kidney diseases ,Malalties cardiovasculars ,Cardiovascular Diseases ,Kidney Diseases ,Stroke ,1103 Clinical Sciences ,Mendelian Randomization Analysis ,kidney diseases ,stroke ,Allmänmedicin ,Cardiovascular diseases ,Cardiovascular System & Hematology ,Malalties del ronyó ,Cardiology and Cardiovascular Medicine ,cardiovascular diseases - Abstract
Background: End-stage renal disease is associated with a high risk of cardiovascular events. It is unknown, however, whether mild-to-moderate kidney dysfunction is causally related to coronary heart disease (CHD) and stroke. Methods: Observational analyses were conducted using individual-level data from 4 population data sources (Emerging Risk Factors Collaboration, EPIC-CVD [European Prospective Investigation into Cancer and Nutrition–Cardiovascular Disease Study], Million Veteran Program, and UK Biobank), comprising 648 135 participants with no history of cardiovascular disease or diabetes at baseline, yielding 42 858 and 15 693 incident CHD and stroke events, respectively, during 6.8 million person-years of follow-up. Using a genetic risk score of 218 variants for estimated glomerular filtration rate (eGFR), we conducted Mendelian randomization analyses involving 413 718 participants (25 917 CHD and 8622 strokes) in EPIC-CVD, Million Veteran Program, and UK Biobank. Results: There were U-shaped observational associations of creatinine-based eGFR with CHD and stroke, with higher risk in participants with eGFR values 105 mL·min –1 ·1.73 m –2 , compared with those with eGFR between 60 and 105 mL·min –1 ·1.73 m –2 . Mendelian randomization analyses for CHD showed an association among participants with eGFR –1 ·1.73 m –2 , with a 14% (95% CI, 3%–27%) higher CHD risk per 5 mL·min –1 ·1.73 m –2 lower genetically predicted eGFR, but not for those with eGFR >105 mL·min –1 ·1.73 m –2 . Results were not materially different after adjustment for factors associated with the eGFR genetic risk score, such as lipoprotein(a), triglycerides, hemoglobin A1c, and blood pressure. Mendelian randomization results for stroke were nonsignificant but broadly similar to those for CHD. Conclusions: In people without manifest cardiovascular disease or diabetes, mild-to-moderate kidney dysfunction is causally related to risk of CHD, highlighting the potential value of preventive approaches that preserve and modulate kidney function.
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- 2022
15. Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
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Tianli, Zou, Junhua, Deng, Xiangdong, Li, Shiyin, Zhang, Lingyan, Chen, Liying, Hao, Jinshan, Zhuang, Heng, Wang, Guihong, Zhang, Shengxiang, Ge, and Kegong, Tian
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Swine ,Hydrolysis ,Virology ,Immunology ,Animals ,Molecular Medicine ,African Swine Fever ,African Swine Fever Virus ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Fluorescent Dyes - Published
- 2022
16. Twelve loci associated with bone density in middle-aged and elderly Chinese: the Shanghai Changfeng Study
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Hailuan Zeng, Jieyu Ge, Wenjie Xu, Hui Ma, Lingyan Chen, Mingfeng Xia, Baishen Pan, Huandong Lin, Sijia Wang, and Xin Gao
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Endocrinology ,genome-wide association study ,sex interaction ,Endocrinology, Diabetes and Metabolism ,Biochemistry (medical) ,Clinical Biochemistry ,bone mineral density ,Biochemistry ,East Asian - Abstract
Context Previous genome-wide association studies (GWASs) of bone mineral density (BMD) were mainly conducted in Europeans. Objective To explore genetic variants that affect BMD and sex differences in a Chinese population. Methods A total of 5428 middle-aged and elderly Chinese were included. Dual-energy X-ray absorptiometry was used to measure BMD at the lumbar spine, and total and specific sites of the hip. A mixed linear model was used to analyze the associations between BMD and autosomal genetic variants, adjusting for age, age squared, sex, and menopausal women (model 1); model 2 was further adjusted for height and weight. A GWAS of osteoporosis in the Biobank Japan (BBJ) project was used for replication. GWAMA software was used to detect the statistical significance of sex differences of estimated effects. Gene annotation and pathway enrichment analysis were performed. Results Women lost BMD at earlier ages and faster than men. The 2 models identified a total of 12 loci that were associated with BMD at any site. Single nucleotide polymorphisms rs72354346, rs2024219, rs1463093, rs10037512, and rs5880932 were successfully replicated in the BBJ. Variations of rs79262027 G>A (VKORC1L1) and rs4795209 A>G (DDX52) were associated with BMD only in men, and rs1239055408 G>GA (KCNJ2) was associated with BMD only in women. Gene enrichment analysis showed that BMD in a Chinese elderly population was mainly related to ossification, bone resorption, sex hormones, and kidney physiology. Conclusion The present GWAS identified 12 loci that were significantly associated with BMD at any site in a Chinese population, and 3 of them showed sex differences in their effects.
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- 2022
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17. The efficacy of paxlovid in elderly patients infected with SARS-CoV-2 omicron variants: Results of a non-randomized clinical trial
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Weijie, Zhong, Xiufeng, Jiang, Xiaosheng, Yang, Tiantong, Feng, Zhixin, Duan, Wei, Wang, Zhaoliang, Sun, Lingyan, Chen, Xin, Nie, Chuanlong, Zhu, Wenchuan, Zhang, and Yi, Li
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General Medicine - Abstract
ObjectiveTo evaluate the efficacy of Paxlovid in treating Chinese elder patients infected with SARS-CoV-2 omicron variants.Materials and methodsWe performed a non-randomized, controlled trial in Shanghai, China. Participants infected with SARS-CoV-2 omicron variants were enrolled. All patients were divided into the Paxlovid group or the control group according to the Chinese guideline (version 9). The nucleic acid shedding time was the primary endpoint.ResultsAccording to the inclusion criteria, 142 patients infected with omicron variants were enrolled, 36 patients who did not receive paxlovid were assigned to the control group, and 106 were in the Paxlovid group. The baseline characteristics were similar in either group. No significant difference in BMI, age, time from onset to patient enrollment, the severity on first admission, vaccination status, comorbidity, first symptoms, and laboratory results were recorded. Compared to the control group, participants in the Paxlovid group had a shorter viral shedding time [11.11 (2.67) vs. 9.32 (2.78), P = 0.001].ConclusionIn Chinese elder patients infected with the variant of SARS-CoV-2 omicron, our data suggest that Paxlovid can significantly reduce the nucleic acid shedding time.
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- 2022
18. Serum type I collagen carboxyl-terminal peptide β glypeptide was positively associated with osteoporosis and skeletal muscle loss
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lingyan chen, jiayu wu, weiying ren, xi li, man luo, and yu hu
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Background Previous studies have presented osteoporosis and sarcopenia frequently occur concomitantly in elderly. Type I collagen carboxyl-terminal peptide β (β-CTX) usually increases in osteoporosis. However, the relationship between β-CTX and muscle mass remains unclear. This study aimed to explore the association between serum β-CTX and the risk of sarcopenia in elderly inpatients. Methods 228 patients > 65 years were recruited in this cross-sectional study. Dual-energy X-ray scanning (DXA) was used to access skeletal muscle and bone mass. Skeletal muscle mass index (SMI) was defined as appendicular skeletal muscle mass (ASM) divided by height2. Serum markers of bone remodeling were detected by electrochemical immunofluorescence assay. Results Serum concentration of β-CTX as well as the prevalence of osteoporosis were higher in low SMI group than in the normal SMI group. Correlation analysis showed that serum β-CTX levels negatively correlated with SMI and bone mass. Across the quartiles of serum β-CTX, total skeletal muscle mass (TSM), ASM, SMI, total bone mass (TBM) and bone mass at various sites including the limbs, spine and pelvis decreased significantly, wherea the prevalence of low SMI and osteoporosis increased. Logistic regression analysis showed the higher quartiles of serum β-CTX had an increased risk of low SMI and osteoporosis, compared to the lowest quartile (the third and fourth quartiles for low SMI and the fourth quartile for osteoporosis). Conclusions With increasing serum β-CTX levels, muscle and bone mass decreased in Chinese elderly inpatients. Serum β-CTX was positively associated with the risk of osteoporosis and muscle loss.
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- 2022
19. Angiotensin-converting enzyme 2 identifies immuno-hot tumors suggesting angiotensin-(1-7) as a sensitizer for chemotherapy and immunotherapy in breast cancer
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Jie Mei, Yun Cai, Rui Xu, Xinqian Yu, Xu Han, Miaomiao Weng, Lingyan Chen, Tao Ma, Tianshu Gao, Fei Gao, Tiansong Xia, Yichao Zhu, and Yan Zhang
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General Biochemistry, Genetics and Molecular Biology - Abstract
Background Angiotensin-converting enzyme 2 (ACE2) is known as a tumor suppressor and lowly expressed in most cancers. The expression pattern and role of ACE2 in breast cancer (BC) have not been deeply elucidated. Methods A systematic pan-cancer analysis was conducted to assess the expression pattern and immunological role of ACE2 based on RNA-sequencing (RNA-seq) data downloaded from The Cancer Genome Atlas (TCGA). The correlation of ACE2 expression and immunological characteristics in the BC tumor microenvironment (TME) was evaluated. The role of ACE2 in predicting the response to therapeutic options was estimated. Moreover, the pharmacodynamic effect of angiotensin-(1–7) (Ang-1–7), the product of ACE2, on chemotherapy and immunotherapy was evaluated on the BALB/c mouse BC model. In addition, the plasma samples from BC patients receiving neoadjuvant chemotherapy were collected and subjected to the correlation analysis of the expression level of Ang-1–7 and the response to neoadjuvant chemotherapy. Results ACE2 was lowly expressed in BC tissues compared with that in adjacent tissues. Interestingly, ACE2 was shown the highest correlation with immunomodulators, tumor-infiltrating immune cells (TIICs), cancer immunity cycles, immune checkpoints, and tumor mutation burden (TMB) in BC. In addition, a high level of ACE2 indicated a low response to endocrine therapy and a high response to chemotherapy, anti-ERBB therapy, antiangiogenic therapy and immunotherapy. In the mouse model, Ang-1–7 sensitized mouse BC to the chemotherapy and anti-PD-1 immunotherapy, which revealed its significant anti-tumor effect. Moreover, a high plasma level of Ang-1–7 was associated with a better response to neoadjuvant chemotherapy. Conclusions ACE2 identifies immuno-hot tumors in BC, and its enzymatic product Ang-1–7 sensitizes BC to the chemotherapy and immunotherapy by remodeling the TME.
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- 2022
20. Presence of sarcopenia identifies a special group of lean NAFLD in middle-aged and older people
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Xiaopeng Zhu, Qingxia Huang, Shuai Ma, Lingyan Chen, Qi Wu, Li Wu, Hui Ma, Xiaoming Li, Qian Li, Qiqige Aleteng, Yu Hu, Wanyuan He, Jian Gao, Huandong Lin, Huiru Tang, Xin Gao, and Mingfeng Xia
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Hepatology - Abstract
Sarcopenia, the age-related loss of muscle mass and function, is closely associated and frequently concomitant with non-alcoholic fatty liver disease (NAFLD). We aimed to investigate the clinical features of the sarcopenic NAFLD patients from middle-aged and older people.A total of 1305 patients with NAFLD from the Shanghai Changfeng Study were included for analysis. Sarcopenia was diagnosed based on the height-adjusted appendicular skeletal muscle mass (ASM/heightAmong the middle-aged and older population, 68.1% of patients with sarcopenia and NAFLD were lean. Sarcopenia was independently associated with increased risk of carotid plaque (OR, 2.22; 95%CI 1.23-4.02) and liver fibrosis (OR, 2.07; 95%CI 1.24-3.44), and the sarcopenic lean NAFLD patients were characterized by a higher risk of carotid plaque (p = 0.008) and liver fibrosis (p = 0.001) than the non-sarcopenic lean NAFLD patients, despite their lower BMI and similar prevalence of metabolic syndrome and diabetes. Further serum metabolomic examination indicated that the sarcopenic lean NAFLD patients presented a distinct metabolomic profile prone to carotid plaque and liver fibrosis, with upregulated serum valine, N-acetylneuraminyl-glycoproteins, lactic acid, small LDL triglycerides and VLDL5 components, and reduced components of HDL4. A sarcopenic characterization score based on above metabolites was established and could also predict increased risk of carotid plaque and liver fibrosis.The presence of sarcopenia identifies a special subgroup of lean NAFLD with increased risk of cardiovascular disease and liver fibrosis clinically.
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- 2022
21. The emerging role of PPAR-alpha in breast cancer
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Zhiwen Qian, Lingyan Chen, Jiayu Liu, Ying Jiang, and Yan Zhang
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Pharmacology ,General Medicine - Published
- 2023
22. Hepatocyte mARC1 promotes fatty liver disease
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Lara C. Lewis, Lingyan Chen, L. Shahul Hameed, Robert R. Kitchen, Cyrielle Maroteau, Shilpa R. Nagarajan, Jenny Norlin, Charlotte E. Daly, Iwona Szczerbinska, Sara Toftegaard Hjuler, Rahul Patel, Eilidh J. Livingstone, Tom N. Durrant, Elisabeth Wondimu, Soumik BasuRay, Anandhakumar Chandran, Wan-Hung Lee, Sile Hu, Barak Gilboa, Megan E. Grandi, Enrique M. Toledo, Abdullah H.A. Erikat, Leanne Hodson, William G. Haynes, Natalie W. Pursell, Ken Coppieters, Jan Fleckner, Joanna M.M. Howson, Birgitte Andersen, and Maxwell A. Ruby
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Hepatology ,Gastroenterology ,Internal Medicine ,Immunology and Allergy - Published
- 2023
23. Type 2 Diabetes Is Causally Associated With Reduced Serum Osteocalcin: A Genomewide Association and Mendelian Randomization Study
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Baishen Pan, Lingyan Chen, Xin Gao, Huandong Lin, Jieyu Ge, Wenjie Xu, Sijia Wang, Hailuan Zeng, Hui Ma, and Mingfeng Xia
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0301 basic medicine ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Osteocalcin ,Population ,030209 endocrinology & metabolism ,Type 2 diabetes ,Bone remodeling ,03 medical and health sciences ,0302 clinical medicine ,Insulin resistance ,Bone Density ,Internal medicine ,Mendelian randomization ,Humans ,Medicine ,Orthopedics and Sports Medicine ,education ,Bone mineral ,education.field_of_study ,biology ,business.industry ,Mendelian Randomization Analysis ,medicine.disease ,030104 developmental biology ,Endocrinology ,Diabetes Mellitus, Type 2 ,biology.protein ,business ,Body mass index ,Genome-Wide Association Study - Abstract
Recent advances indicate that bone and energy metabolism are closely related. However, little direct evidence on causality has been provided in humans. We aimed to assess the association of three bone-related biomarkers-25 hydroxyvitamin D (25OHD), parathyroid hormone (PTH), and osteocalcin (OCN)-with several metabolic phenotypes and investigate any causal relevance to the associations using a Mendelian randomization (MR) study. Serum 25OHD, PTH, and total OCN were measured at baseline in 5169 eligible Chinese participants in Changfeng study. Partial correlation and bivariate GREML analysis were used to estimate phenotypic and genetic correlations, respectively. Multiple linear regression and logistic regression were used to assess linear associations. Genomewide association analysis (GWAS) was performed. Bidirectional two-sample MR analyses were conducted to examine causal relationships between OCN and body mass index (BMI), diastolic blood pressure (DBP), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), glycated hemoglobin A1c (HbA1c), and type 2 diabetes (T2DM), using our GWAS result of OCN and GWAS statistics from Biobank Japan project (BBJ) and the largest meta-analysis of T2DM GWAS in East Asian population. Circulating OCN was significantly associated with higher DBP and HDL-C and decreased TG, blood glucose level, insulin resistance, liver fat content, bone mineral density, BMI, and a favorable body fat distribution pattern. GWAS identified one novel serum PTH locus and two novel serum OCN loci, explaining 0.81% and 1.98% of variances of PTH and OCN levels, respectively. MR analysis suggested a causal effect of T2DM on lower circulating OCN concentration (causal effect: -0.03; -0.05 to -0.01; p = 0.006 for T2DM_BBJ and -0.03; -0.05 to -0.01; p = 0.001 for T2DM_EAS). These findings indicate that T2DM might impact bone remodeling and provide a resource for understanding complex relationships between osteocalcin and metabolic (and related) traits in humans. © 2021 American Society for Bone and Mineral Research (ASBMR).
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- 2021
24. Ciclopirox drives growth arrest and autophagic cell death through STAT3 in gastric cancer cells
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Lingyan Chen, Dejian Chen, Jiwei Li, Lipeng He, Ting Chen, Dandan Song, Shuang Shan, Jiaxin Wang, Xiaoang Lu, and Bin Lu
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STAT3 Transcription Factor ,Cancer Research ,Cellular and Molecular Neuroscience ,Stomach Neoplasms ,Autophagic Cell Death ,Immunology ,Serine ,Humans ,Tyrosine ,Cell Biology ,Ciclopirox ,Phosphorylation - Abstract
Ciclopirox (CPX), an antifungal drug, has recently been identified as a promising agent for cancer treatment. However, the effects and underlying mechanism of CPX as an antitumor agent of gastric cancer (GC) remain largely unknown. Here, we found that CPX dramatically suppresses GC xenograft growth in vitro via inhibiting proliferation and stimulating autophagic cell death rather than apoptosis. Moreover, CPX (20 mg/kg, intraperitoneally) substantially inhibits GC xenograft tumor growth in vivo. Mechanistically, CPX promotes growth arrest and autophagic cell death through suppressing the phosphorylation of signal transducers and activators of transcription 3 (STAT3) at tyrosine 705 (Tyr705) and serine 727 (Ser727) sites, respectively. Additionally, CPX induces STAT3 ubiquitination, which subsequently leads to a decrease in the p-STAT3 (Ser727) level. On the other hand, CPX represses the p-STAT3 (Tyr705) level via p-Src (Tyr416) inhibition. Collectively, our findings unmask a novel mechanism by which CPX regulates growth and autophagic cell death in GC cells via regulating the phosphorylation of STAT3 both at Tyr705 and Ser727 residues, and suggest that CPX may be a potential treatment for GC.
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- 2022
25. A Novel DNA Repair Gene Signature for Immune Checkpoint Inhibitor-Based Therapy in Gastric Cancer
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Binbin, Yuan, Chengfei, Jiang, Lingyan, Chen, Lihui, Wen, Jinlong, Cui, Min, Chen, Shu, Zhang, Lin, Zhou, Yimeng, Cai, Jian-Hua, Mao, Xiaoping, Zou, Bo, Hang, and Pin, Wang
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gastric cancer ,Cell Biology ,immune checkpoint blockade ,DNA repair gene signature ,immune checkpoint inhibitors ,Vaccine Related ,Rare Diseases ,Good Health and Well Being ,5.1 Pharmaceuticals ,Genetics ,Immunization ,immunotherapy ,score system ,Genetic Testing ,Development of treatments and therapeutic interventions ,prognostic biomarker ,Digestive Diseases ,Cancer ,Developmental Biology - Abstract
Gastric cancer is a heterogeneous group of diseases with only a fraction of patients responding to immunotherapy. The relationships between tumor DNA damage response, patient immune system and immunotherapy have recently attracted attention. Accumulating evidence suggests that DNA repair landscape is a significant factor in driving response to immune checkpoint blockade (ICB) therapy. In this study, to explore new prognostic and predictive biomarkers for gastric cancer patients who are sensitive and responsive to immunotherapies, we developed a novel 15-DNA repair gene signature (DRGS) and its related scoring system and evaluated the efficiency of the DRGS in discriminating different molecular and immune characteristics and therapeutic outcomes of patients with gastric adenocarcinoma, using publicly available datasets. The results demonstrated that DRGS high score patients showed significantly better therapeutic outcomes for ICB compared to DRGS low score patients (p < 0.001). Integrated analysis of multi-omics data demonstrated that the patients with high DRGS score were characteristic of high levels of anti-tumor lymphocyte infiltration, tumor mutation burden (TMB) and PD-L1 expression, and these patients exhibited a longer overall survival, as compared to the low-score patients. Results obtained from HPA and IHC supported significant dysregulation of the genes in DRGS in gastric cancer tissues, and a positive correlation in protein expression between DRGS and PD-L1. Therefore, the DRGS scoring system may have implications in tailoring immunotherapy in gastric cancers. A preprint has previously been published (Yuan et al., 2021).
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- 2022
26. The relationship between skeletal muscle mass and bone mass at different sites in older adults
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Lingyan Chen, Jiayu Wu, Weiying Ren, Xi Li, Man Luo, and Yu Hu
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Nutrition and Dietetics ,Medicine (miscellaneous) - Abstract
Introduction: It has been well recognized that sarcopenia is closely related with osteoporosis, while the relationship between bone mass at different sites and muscle mass remains largely unexplored. This study aims to explore the relationship between bone mass at different sites and skeletal muscle mass in older adults. Methods: A total of 228 patients over 65 years old were enrolled in this study, and then 180 valid participants with accessible dual-energy X-ray absorptiometry (DXA) scanning data and absence of malignant tumors, mobility disorders, serious liver and kidney disease, and cardiac insufficiency were selected (138 male and 42 female). These individuals were further divided into control group and low skeletal muscle mass index (SMI) group. DXA scanning was used to access skeletal muscle mass and bone mass. SMI or body mass index was defined as appendicular muscle mass or weight divided by squared height, respectively. Low SMI 2 in male or 2 in female was defined as low SMI; while the bone density score at or below 2.5 standard deviations (T-score) below normal peak bone values, was regarded as osteoporosis. Results: The average age of enrolled patients was 82.72 years; the ratios of osteoporosis, low SMI, and low SMI with osteoporosis were 48.8%, 23.3%, and 15.0%, respectively. Compared with the control group, the prevalence of osteoporosis was higher, and the total bone mass and bone mass at various sites including limbs, spine, and pelvis, were all lower in low SMI group. Correlation analysis showed that SMI was positively related with total bone mass and bone mass at various sites. Covariance analysis showed that only total bone mass and appendicular bone mass decreased with decreasing SMI. After multiple adjustment, osteoporosis was positively related with the prevalence of low SMI, as evidenced by logistic regression analysis (odds ratio = 1.33, 95% confidential interval: 1.04–3.24, p = 0.045). Furthermore, compared with the highest quartile of appendicular bone mass, the lowest quartile was related with the increasing prevalence of low SMI (odds ratio = 7.29, 95% confidential interval: 1.21–67.45, p = 0.042). Conclusion: Compared with the other sites, the bone mass reduction at limbs of older adults was positively associated with skeletal muscle loss. It may be more beneficial to increase bone mass at the limbs for improved sarcopenia prevention and therapy. Further investigations are needed to explore the effects of other confounders (e.g., energy, calcium and vitamin D intake, and physical activity) on the osteoporosis and sarcopenia in older adults.
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- 2022
27. Sarcopenia, sarcopenic overweight/obesity and risk of cardiovascular disease and cardiac arrhythmia: A cross-sectional study
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Hui Ma, Jian Gao, Yu Hu, Xin Gao, Mingfeng Xia, Li Wu, Lingyan Chen, Xiaoming Li, Qiqige Aleteng, Huandong Lin, Wanyuan He, and Qian Li
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Carotid Artery Diseases ,Male ,0301 basic medicine ,China ,Sarcopenia ,medicine.medical_specialty ,Cross-sectional study ,Infarction ,030209 endocrinology & metabolism ,Disease ,Critical Care and Intensive Care Medicine ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Prevalence ,medicine ,Humans ,Obesity ,Prospective Studies ,cardiovascular diseases ,Myocardial infarction ,030109 nutrition & dietetics ,Nutrition and Dietetics ,business.industry ,Cardiac arrhythmia ,Arrhythmias, Cardiac ,Atrial fibrillation ,Middle Aged ,Overweight ,medicine.disease ,Cross-Sectional Studies ,Cardiovascular Diseases ,Heart Disease Risk Factors ,Heart failure ,cardiovascular system ,Cardiology ,Female ,business - Abstract
Sarcopenia is an age-dependent skeletal muscle disorder that is common in patients with heart failure. The current study aimed to investigate the associations of sarcopenia with carotid atherosclerosis, cardiovascular disease and cardiac arrhythmia in a middle-aged and elderly population without clinical heart failure.A total of 2432 participants (992 men and 1440 women) from Shanghai Changfeng Study were included for analysis. The degree of sarcopenia was measured using height-adjusted appendicular skeletal muscle mass (ASM/heightSarcopenia was associated with higher prevalence of carotid atherosclerosis (26.4% vs 20.4%, P = 0.027), myocardial infarction (4.0% vs 1.1%, P = 0.001), and premature ventricular contraction (4.0% vs 2.0%, P = 0.034) in the participants with normal body weight, and higher prevalence of carotid atherosclerosis (45.0% vs 31.2%, P = 0.016), myocardial infarction (10.0% vs 4.3%, P = 0.020) and atrial fibrillation (7.5% vs 1.3%, P 0.001) in those with overweight/obese status. After adjustment for age, gender, cigarette smoking, alcohol drinking, menopausal status in women and other metabolic and inflammatory confounding factors, sarcopenia was independently associated with the risk of myocardial infarction in the whole population, and the risk of atrial fibrillation in the overweight/obese participants (all P 0.05). Compared with nonsarcopenic lean participants, the risk of myocardial infarction was gradually increased in sarcopenic lean (OR 3.08 [1.28-7.45], P = 0.012) and sarcopenic overweight/obese participants (OR 4.07 [1.31-12.62], P = 0.015). For the atrial fibrillation, the participants with either sarcopenia or overweight/obesity alone showed no higher risk. However, concomitant sarcopenia and overweight/obesity was associated with approximately 5-fold risk of atrial fibrillation (OR 5.68 [1.34-24.12], P = 0.019) after multiple adjustment.Sarcopenia is associated with myocardial infarction and atrial fibrillation in middle-aged and elderly adults without clinical heart failure.
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- 2021
28. Retrieving chlorophyll content and equivalent water thickness of Moso bamboo (Phyllostachys pubescens) forests under Pantana phyllostachysae Chao-induced stress from Sentinel-2A/B images in a multiple LUTs-based PROSAIL framework
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Zhanghua Xu, Anqi He, Yiwei Zhang, Zhenbang Hao, Yifan Li, Songyang Xiang, Bin Li, Lingyan Chen, Hui Yu, Wanling Shen, Xuying Huang, Xiaoyu Guo, and Zenglu Li
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Ecology ,Forestry ,Ecology, Evolution, Behavior and Systematics ,Nature and Landscape Conservation - Published
- 2023
29. Osteocalcin and Non‐Alcoholic Fatty Liver Disease: Lessons From Two Population‐Based Cohorts and Animal Models
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Hui Ma, Xiaopeng Zhu, Linshan Zhang, Mingfeng Xia, Xinxia Chang, Jian Gao, Baishen Pan, Xiaoyang Sun, Hongmei Yan, Li Wu, Huandong Lin, Wanyuan He, Xiqi Hu, Shunxing Rong, Yu Hu, Hailuan Zeng, Xin Gao, Hua Bian, Xiaoming Li, and Lingyan Chen
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Male ,musculoskeletal diseases ,China ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Osteocalcin ,Bone remodeling ,Mice ,Non-alcoholic Fatty Liver Disease ,Internal medicine ,Animals ,Humans ,Medicine ,Orthopedics and Sports Medicine ,Prospective Studies ,medicine.diagnostic_test ,biology ,business.industry ,Fatty liver ,Odds ratio ,medicine.disease ,Rats ,Disease Models, Animal ,Endocrinology ,Liver ,Liver biopsy ,Cohort ,Lipogenesis ,biology.protein ,Female ,Steatosis ,business - Abstract
Osteocalcin regulates energy metabolism in an active undercarboxylated/uncarboxylated form. However, its role on the development of non-alcoholic fatty liver disease (NAFLD) is still controversial. In the current study, we investigated the causal relationship of circulating osteocalcin with NAFLD in two human cohorts and studied the effect of uncarboxylated osteocalcin on liver lipid metabolism through animal models. We analyzed the correlations of serum total/uncarboxylated osteocalcin with liver steatosis/fibrosis in a liver biopsy cohort of 196 participants, and the causal relationship between serum osteocalcin and the incidence/remission of NAFLD in a prospective community cohort of 2055 subjects from Shanghai Changfeng Study. Serum total osteocalcin was positively correlated with uncarboxylated osteocalcin (r = 0.528, p < .001). Total and uncarboxylated osteocalcin quartiles were inversely associated with liver steatosis, inflammation, ballooning, and fibrosis grades in both male and female participants (all p for trend
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- 2020
30. Berberine Down-Regulated Myostatin Expression and Facilitated Metabolism via Smad Pathway in Insulin Resistant Mice
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Yu Hu, Xiaojuan Su, and Lingyan Chen
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medicine.medical_specialty ,Adipose tissue ,030209 endocrinology & metabolism ,Myostatin ,030204 cardiovascular system & hematology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Insulin resistance ,Berberine ,Internal medicine ,Internal Medicine ,medicine ,Protein kinase B ,Pharmacology ,Glucose tolerance test ,medicine.diagnostic_test ,biology ,business.industry ,Insulin tolerance test ,Skeletal muscle ,medicine.disease ,Endocrinology ,medicine.anatomical_structure ,chemistry ,biology.protein ,business - Abstract
Objective To explore the effect of berberine (BBR) on skeletal muscle mass and metabolism and the possible mechanism. Methods Eight-week-old male C57BL/6 mice were fed with a high-fat diet (HFD) for 8 weeks to establish the insulin resistance obesity model. Then, mice were randomly divided into two groups (normal chow diet (NCD) and HFD), while NCD and HFD were further classified into two groups respectively, which were NCD+CS (Carmellose Sodium), NCD+BBR, HFD+CS and HFD+BBR. After the BBR intervention, insulin tolerance test (ITT) and glucose tolerance test (GTT) were carried out. Metabolic parameters and inflammatory biomarkers were detected. Various parts of adipose tissue and gastrocnemius were separated and measured. The gastrocnemius muscle was selected for tissue staining. The mRNA expression of myostatin (Mstn) was tested by quantitative real-time PCR (RT-PCR). Western blotting was performed to detect the expression of Mstn, phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), small ribonucleoprotein particle protein 2 (Smd2), small ribonucleoprotein particle protein 3 (Smd3), and small ribonucleoprotein particle protein 4 (Smd4). Results Both body weights (P
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- 2020
31. Associations of poor sleep quality, chronic pain and depressive symptoms with frailty in older patients: is there a sex difference?
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Shanshan Shen, Xingkun Zeng, Yinghong Yang, Huilan Guan, Lingyan Chen, and Xujiao Chen
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Male ,Sex Characteristics ,Sleep Quality ,Frailty ,Depression ,Sleep Initiation and Maintenance Disorders ,Humans ,Female ,Geriatrics and Gerontology ,Chronic Pain ,Aged - Abstract
Background Sleep disturbance, chronic pain and depressive symptoms later in life are modifiable risk factors and may contribute to frailty. However, much less is known about sex differences in the association between these concurrent symptoms and frailty in older patients. Therefore, we conducted this study to explore the associations of poor sleep quality, chronic pain, and depressive symptoms with frailty in older patients, and the sex-specific associations. Methods In an observational population-based study, 540 older hospitalized patients from Zhejiang Hospital in China were enrolled. We collected data on poor sleep quality, pain, depressive symptoms and frailty using the Pittsburgh Sleep Quality Index, the Numerical Rating Scale, the 15-item Geriatric Depression Scale, and the Clinical Frailty Scale. Multivariate logistic regression models were used to explore the total sample and sex-specific associations among symptom burdens, symptom combination patterns and symptom counts, and frailty. Results After adjusting for the potential covariates, concurrent poor sleep quality and depressive symptoms (OR = 4.02, 95% CI 1.57–10.26), concurrent poor sleep quality and chronic pain (OR = 2.05, 95% CI 1.04–4.05), and having three symptoms (OR = 3.52, 95% CI 1.19–10.44) were associated with a higher likelihood of frailty in older inpatients. In addition, older patients with 2 or 3 symptoms (2 and 3 vs. 0 symptoms) had a higher risk of frailty, and the odds ratios were 2.40 and 3.51, respectively. Interaction analysis and sex-stratified associations exhibited conflicting results. The nonsignificant effect of the interaction of sex and symptoms on frailty, but not the sex-stratified associations, showed that individual symptoms, symptom combination patterns, and symptom counts were associated with elevated risks of frailty in older male patients, but not in older female patients. Conclusions Increased symptom burdens were associated with a higher risk of frailty in older inpatients, especially in those with poor sleep quality concurrent with at least one of the other two symptoms. Thus, a multidisciplinary program addressing these common symptoms is required to reduce adverse outcomes.
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- 2022
32. Study on superconducting Li-Se-H hydrides
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BingYu Li, WenHua Yang, HaiLiang Chen, LiZhen Zhao, LingYan Chen, KaiPing Yang, and WenCai Lu
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General Physics and Astronomy ,Physical and Theoretical Chemistry - Abstract
Crystal structures and superconducting properties of C2 LiSeH4 and Pmm2 LiSeH6 were studied under pressure using first-principles calculations.
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- 2022
33. IQGAP1 is positively correlated with PD-L1 and regulates its expression via mediating STAT proteins phosphorylation
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Yu Chen, Jie Mei, Ping Zhang, Jiayu Liu, Lingyan Chen, Liwei Wu, and Yan Zhang
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Pharmacology ,ErbB Receptors ,Epidermal Growth Factor ,ras GTPase-Activating Proteins ,Immunology ,Immunology and Allergy ,Humans ,Female ,Phosphorylation ,B7-H1 Antigen - Abstract
IQ motif containing GTPase activating protein 1 (IQGAP1) is a scaffold protein to transduce multiple signals from the extracellular microenvironment. However, whether IQGAP1 mediates EGF-induced cancer progression has been not observed. In this research, we explored the immunological correlations of IQGAP1. IQGAP1 was up-regulated in most cancer and related to poor prognosis in ovarian cancer. In addition, IQGAP1 was correlated with immune-related genes and tumor-infiltrating lymphocytes. IQGAP1 was a novel EGF-response gene, which was up-regulated by EGF. As the downstream of EGFR, IQGAP1 mediated EGF-induced proliferation, migration and invasion of tumor cells and was essential for EGF-induced PD-L1 expression as well. Mechanically, IQGAP1 interacted with STATs and mediated their phosphorylation. Furthermore, Curcumin inhibited EGFR/IQGAP1 axis to down-regulate PD-L1 and suppress cancer progression. To sum up, IQGAP1 responds to EGF stimulation and mediates EGF-induced phosphorylation of STATs, which is a novel promising target for blocking the EGF-induced cancer progression.
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- 2022
34. Cover Image
- Author
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Mingfeng Xia, Shuai Ma, Qingxia Huang, Hailuan Zeng, Jieyu Ge, Wenjie Xu, Qi Wu, Li Wu, Xiaoming Li, Hui Ma, Lingyan Chen, Qian Li, Qiqige Aleteng, Yu Hu, Wanyuan He, Baishen Pan, Huandong Lin, Yan Zheng, Sijia Wang, Huiru Tang, and Xin Gao
- Subjects
Hepatology ,Gastroenterology ,Pharmacology (medical) - Published
- 2022
35. Scaffold with Orientated Microtubule Structure Containing Polylysine-Heparin Sodium Nanoparticles for the Controlled Release of TGF-β1 in Cartilage Tissue Engineering
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Mingmao Chen, Chunrong Yang, Yingying Wang, Jianhua Wang, Xiaomin Sun, Qiqing Zhang, Lingyan Chen, and Chenguang Huang
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musculoskeletal diseases ,Scaffold ,Sodium ,Biochemistry (medical) ,Biomedical Engineering ,chemistry.chemical_element ,Nanoparticle ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Controlled release ,Cartilage tissue engineering ,0104 chemical sciences ,Biomaterials ,chemistry ,Microtubule ,Biophysics ,Polylysine-heparin ,0210 nano-technology ,Transforming growth factor - Abstract
Articular cartilage defects cannot adequately self-repair because of avascular and complex tissues. Although various scaffold materials have been reported to promote cartilage repair, challenges remain in this field. In the present study, an orientated microtubules scaffold that consisted of collagen, chitosan, silk fibroin, and polylysine-heparin sodium nanoparticles containing transforming growth factor β1 (TGF-β1), was constructed using a unidirectional freeze-drying method. The collagen/chitosan/0.5silk fibroin scaffolds showed optimal properties via the evaluation of physicochemical characterization, mechanical properties and biocompatibility. The collagen/chitosan/0.5silk fibroin scaffolds containing polylysine-heparin sodium nanoparticles loaded with TGF-β1 (COL/CS/0.5SF-TPHNs) could control the slow release of TGF-β1. Simultaneously, the in vitro COL/CS/0.5SF-TPHNs scaffolds exhibited not only an excellent biocompatibility to support mouse mesenchymal stem cells (mBMSCs) adhesion and proliferation, but also exhibited excellent repair effect in cartilage defects of rabbit models in vivo. Our results showed that the COL/CS/0.5SF-TPHNs scaffolds have the potential to induce articular cartilage formation, mainly because TGF-β1 could induce the mBMSCs to differentiate into chondrocytes and osteoblasts. Consequently, the current study can achieve cartilage repair, and COL/CS/0.5SF-TPHNs scaffolds are expected to be potential composites for cartilage tissue engineering.
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- 2022
36. Association between motoric cognitive risk syndrome and frailty among older Chinese adults
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Liyu Xu, Zixia Liu, Xujiao Chen, Jiaojiao Chu, Xiushao Wu, Yinghong Yang, Lingyan Chen, Shanshan Shen, and Xingkun Zeng
- Subjects
Gerontology ,Male ,Aging ,Slow gait ,Cognitive complaints ,Poison control ,lcsh:Geriatrics ,Logistic regression ,03 medical and health sciences ,0302 clinical medicine ,Cognition ,Medicine ,Humans ,Cognitive Dysfunction ,030212 general & internal medicine ,Older adult ,Gait ,Geriatric Assessment ,Aged ,Movement Disorders ,Frailty ,business.industry ,Confounding ,Odds ratio ,Syndrome ,Middle Aged ,Confidence interval ,lcsh:RC952-954.6 ,Cross-Sectional Studies ,Observational study ,Female ,Geriatrics and Gerontology ,business ,Cognition Disorders ,030217 neurology & neurosurgery ,hormones, hormone substitutes, and hormone antagonists ,Research Article - Abstract
Background Motoric cognitive risk syndrome (MCR) is a newly proposed predementia syndrome incorporating subjective cognitive complaints and slow gait. Previous studies have reported that subjective cognitive complaints and slow gait are associated with frailty in cognitively unimpaired older adults, but little is known about the link between MCR and frailty in older adults. Therefore, the aim of the study was to explore the associations of MCR and its components with frailty in older Chinese adults. Methods In an observational cross-sectional study, a total of 429 older adults aged 60 years and older were admitted to the geriatric department. According to MCR criteria, all participants were classified into 4 groups: 1) the MCR group; 2) the subjective cognitive complaints only group; 3) the slow gait only group; and 4) the healthy control group. Physical frailty was assessed by the Clinical Frailty Scale (CFS). Multivariate logistic regression analysis was used to examine the association between MCR and frailty in older adults. Results The prevalence rates of subjective cognitive complaints, slow gait and MCR were 15.9, 10.0 and 4.0%, respectively. After adjusting for confounding variables, the logistic regression analysis showed that slow gait (odds ratio [OR]: 3.40, 95% confidence interval [CI]: 1.40–8.23, P = 0.007) and MCR (OR: 5.53, 95% CI: 1.46–20.89, P = 0.012) were independently associated with frailty, but subjective cognitive complaints were not. Conclusions MCR and slow gait were significantly associated with frailty in older Chinese adults. Further studies should prospectively determine the causal relationship between MCR and frailty.
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- 2020
37. Association Between Body Composition and Frailty in Elder Inpatients
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Liyu Xu, Yinghong Yang, Shanshan Shen, Xingkun Zeng, Xiufang Hong, Lingyan Chen, Zixia Liu, Jie Zhang, and Xujiao Chen
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medicine.medical_specialty ,Waist ,business.industry ,Body water ,General Medicine ,Anthropometry ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Basal metabolic rate ,medicine ,Sarcopenic obesity ,030212 general & internal medicine ,Geriatrics and Gerontology ,medicine.symptom ,Underweight ,business ,Body mass index ,030217 neurology & neurosurgery ,Abdominal obesity - Abstract
Purpose The study aimed to investigate the association between body composition and frailty in elder inpatients. Patients and Methods This is a cross-sectional study including 656 elder inpatients (275 females and 381 males) aged ≥65 years, from department of geriatrics of Zhejiang Hospital between January 2018 and March 2019. Sociodemographic, health-related data and anthropometric measurements were evaluated. Body composition was assessed by bioimpedance analysis (BIA), mainly including skeletal muscle mass, body fat mass, total body water, fat-free mass,percent body fat, basal metabolic rate. Frailty was assessed by Clinical Frailty Scale (CFS). Univariate logistic regression was used to analyze the association between body composition and frailty. Results Frailty was present in 43.9% of the participants. Frail inpatients showed higher waist circumference, body fat mass and percent body fat, non-frail inpatients showed greater upper arm circumference, calf circumference, skeletal muscle mass, total body water, fat-free mass and basal metabolic rate. Subjects with underweight (body mass index (BMI)
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- 2020
38. Rice straw biochar impact on physiological and biochemical attributes of Fokienia hodginsii in acidic soil
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Zhiwen Deng, Muhammad Waqqas Khan Tarin, Lu Shen, Yushan Zheng, Jundong Rong, Lingyan Chen, Jingwen Li, Lili Fan, Jinli Lai, and Tianyou He
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040101 forestry ,010504 meteorology & atmospheric sciences ,Fokienia hodginsii ,Forestry ,04 agricultural and veterinary sciences ,Rice straw ,01 natural sciences ,Agronomy ,Productivity (ecology) ,Soil pH ,Biochar ,0401 agriculture, forestry, and fisheries ,Environmental science ,0105 earth and related environmental sciences - Abstract
Increasing the forest's productivity is a global concern and there is a need to develop innovative technologies. The use of biochar (BC) in the restoration of degraded sites is mounting. Therefore,...
- Published
- 2020
39. Resveratrol inhibits ACHN cells via regulation of histone acetylation
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Lingyan Chen, Wenjing Wang, Peizheng Lin, and Lili Dai
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Functional role ,renal cell carcinoma ,endocrine system diseases ,Cell Survival ,proliferation ,mmp-2/-9 ,Pharmaceutical Science ,RM1-950 ,Resveratrol ,urologic and male genital diseases ,030226 pharmacology & pharmacy ,01 natural sciences ,Histones ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Movement ,Renal cell carcinoma ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,Carcinoma, Renal Cell ,neoplasms ,Pharmacology ,biology ,organic chemicals ,Cell Cycle ,Acetylation ,General Medicine ,medicine.disease ,Kidney Neoplasms ,female genital diseases and pregnancy complications ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Histone ,Matrix Metalloproteinase 9 ,Complementary and alternative medicine ,chemistry ,Cancer research ,biology.protein ,Matrix Metalloproteinase 2 ,Molecular Medicine ,Therapeutics. Pharmacology ,Research Article - Abstract
Context: The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. Objective: To explore the functional role of resveratrol in RCC. Materials and methods: Functional experiments were performed to determine proliferatio n of ACHN cells with treatment of resveratrol (0, 7.8125, 15.625, 31.25 and 62.5 μg/mL, for 12, 24 and 48 h of culture) or 0.1 μM SAHA. The enzyme activities of MMP-2/-9 were measured by gelatine zymography and histone acetylation by Western blot. Results: When the cells were treated with 15.625, 31.25 and 62.5 μg/mL resveratrol, ACHN cells viability was 73.2 ± 3.5%, 61.4 ± 3.1%, 50.2 ± 4.7% for 12 h, 62.7 ± 4.5%, 52.4 ± 5.5%, 40.2 ± 3.8% for 24 h, and 60.8 ± 3.7%, 39.4 ± 5.1%, 37.6 ± 2.7% for 48 h, and the wound closure (%) of migration was increased from 0.6 to 0.7, 0.85, 0.9 for 12 h and from 0.23 to 0.3, 0.48, 0.59 for 24 h. The invasion rate was 8.5 ± 0.9%, 7.4 ± 0.3% and 5.8 ± 0.6%, and cell cycle was arrested at G1 from 42.5 ± 2.9% to 55.3 ± 5.7%, 59.8 ± 3.4%, 68.7 ± 4.6%. MMP-2/-9 expression (p
- Published
- 2020
40. Allele-aware chromosome-scale assembly of the allopolyploid genome of hexaploid Ma bamboo (Dendrocalamus latiflorus Munro)
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Yushan Zheng, Deming Yang, Jundong Rong, Liguang Chen, Qiang Zhu, Tianyou He, Lingyan Chen, Jing Ye, Lili Fan, Yubang Gao, Hangxiao Zhang, and Lianfeng Gu
- Subjects
Polyploidy ,Plant Science ,Poaceae ,Transcriptome ,Biochemistry ,General Biochemistry, Genetics and Molecular Biology ,Alleles ,Chromosomes ,Genome, Plant - Abstract
Dendrocalamus latiflorus Munro is a woody clumping bamboo with rapid shoot growth. Both genetic transformation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing techniques are available for D. latiflorus, enabling reverse genetic approaches. Thus, D. latiflorus has the potential to be a model bamboo species. However, the genome sequence of D. latiflorus has remained unreported due to its polyploidy and large genome size. Here, we sequenced the D. latiflorus genome and assembled it into three allele-aware subgenomes (AABBCC), representing the largest genome of a major bamboo species. We assembled 70 allelic chromosomes (2, 737 Mb) for hexaploid D. latiflorus using both single-molecule sequencing from the Pacific Biosciences (PacBio) Sequel platform and chromosome conformation capture sequencing (Hi-C). Repetitive sequences comprised 52.65% of the D. latiflorus genome. We annotated 135 231 protein-coding genes in the genome based on transcriptomes from eight different tissues. Transcriptome sequencing using RNA-Seq and PacBio single-molecule real-time long-read isoform sequencing revealed highly differential alternative splicing (AS) between non-abortive and abortive shoots, suggesting that AS regulates the abortion rate of bamboo shoots. This high-quality hexaploid genome and comprehensive strand-specific transcriptome datasets for this Poaceae family member will pave the way for bamboo research using D. latiflorus as a model species.
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- 2021
41. Genetically predicted levels of the human plasma proteome and risk of stroke: a Mendelian Randomization study
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Adam S. Butterworth, Cathryn M. Lewis, John Danesh, Nicholas A. Watkins, James E. Peters, Lingyan Chen, David J. Roberts, Matthew Traylor, Praveen Surendran, Stephen Burgess, Joanna M. M. Howson, Emanuele Di Angelantonio, Willem H. Ouwehand, Elodie Persyn, Hugh S. Markus, Paola G. Bronson, Ekaterina Yonova-Doing, Bram Prins, and Savita Karthikeyan
- Subjects
Blood pressure ,business.industry ,Mendelian randomization ,medicine ,Genome-wide association study ,Atrial fibrillation ,Type 2 diabetes ,medicine.disease ,Bioinformatics ,business ,Stroke ,Body mass index ,Hyperintensity - Abstract
Proteins are the effector molecules of biology and are the target of most drugs. To identify proteins and related pathways that may play a causal role in stroke pathogenesis, we used Mendelian randomisation (MR). We tested potential causal effects of 308 plasma proteins (measured in 4,994 blood donors from the INTERVAL study) on stroke outcomes (derived from the MEGASTROKE GWAS) in a two-sample MR framework and assessed whether these associations could be mediated by cardiovascular risk factors. We extended the analysis to identify whether pharmacological targeting of these proteins might have potential adverse side-effects or beneficial effects for other conditions through Phenome-wide MR (Phe-MR) in UK Biobank. MR showed an association between stroke and genetically predicted plasma levels of TFPI, IL6RA, MMP12, CD40, TMPRSS5 and CD6 (P≤1.62⋅10−4). We identified six risk factors (atrial fibrillation, body mass index, smoking, blood pressure, white matter hyperintensities and type 2 diabetes) that were associated with stroke (P≤0.0071) using MR. The association of TFPI, IL6RA and TMPRSS5 with stroke could be mediated by these risk factors, such as body mass index, white matter hyperintensity and atrial fibrillation. Thirty-six additional proteins were potentially causal for one or more of these risk factors. The Phe-MR suggested that targeting TFPI could have potential beneficial effects on other disorders of arteries and hyperlipidaemia in addition to stroke. Our results highlight novel causal pathways and potential therapeutic targets for stroke.
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- 2021
42. Genetically predicted levels of the human plasma proteome and risk of stroke: a Mendelian Randomization study
- Author
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Lingyan Chen, James E. Peters, Bram Prins, Elodie Persyn, Matthew Traylor, Praveen Surendran, Savita Karthikeyan, Ekaterina Yonova-Doing, Emanuele Di Angelantonio, David J. Roberts, Nicholas A. Watkins, Willem H. Ouwehand, John Danesh, Cathryn M. Lewis, Paola G. Bronson, Hugh S. Markus, Stephen Burgess, Adam S. Butterworth, and Joanna M. M. Howson
- Abstract
Proteins are the effector molecules of biology and are the target of most drugs. To identify proteins and related pathways that may play a causal role in stroke pathogenesis, we used Mendelian randomisation (MR). We tested potential causal effects of 308 plasma proteins (measured in 4,994 blood donors from the INTERVAL study) on stroke outcomes (derived from the MEGASTROKE GWAS) in a two-sample MR framework and assessed whether these associations could be mediated by cardiovascular risk factors. We extended the analysis to identify whether pharmacological targeting of these proteins might have potential adverse side-effects or beneficial effects for other conditions through Phenome-wide MR (Phe-MR) in UK Biobank.MR showed an association between stroke and genetically predicted plasma levels of TFPI, IL6RA, MMP12, CD40, TMPRSS5 and CD6 (P≤1.62×10−4). We identified six risk factors (atrial fibrillation, body mass index, smoking, blood pressure, white matter hyperintensities and type 2 diabetes) that were associated with stroke (P≤0.0071) using MR. The association of TFPI, IL6RA and TMPRSS5 with stroke could be mediated by these risk factors, such as body mass index, white matter hyperintensity and atrial fibrillation. Thirty-six additional proteins were potentially causal for one or more of these risk factors. The Phe-MR suggested that targeting TFPI could have potential beneficial effects on other disorders of arteries and hyperlipidaemia in addition to stroke. Our results highlight novel causal pathways and potential therapeutic targets for stroke.
- Published
- 2021
43. A 15-DNA Repair Gene Prognostic Signature for Immunotherapy in Gastric Cancer
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Min Chen, Xiaoping Zou, Jian-Hua Mao, Bo Hang, Yimeng Cai, Shu Zhang, Chengfei Jiang, Jinlong Cui, Binbin Yuan, Pin Wang, and Lingyan Chen
- Subjects
Prognostic signature ,DNA repair ,business.industry ,medicine.medical_treatment ,Cancer research ,medicine ,Cancer ,Prognostic biomarker ,Immunotherapy ,medicine.disease ,business ,health care economics and organizations ,oncology_oncogenics - Abstract
Gastric cancer is a heterogeneous group of diseases with only a fraction of patients responds to immunotherapy. The relationships between tumor DNA damage response, the immune system and immunotherapy have recently attracted attention. Accumulating evidence indicate that DNA repair landscape is a significant factor in driving response to immune checkpoint blockade (ICB) therapy. In this study, to explore new prognostic and predictive biomarkers for gastric cancer patients who are sensitive and responsible to immunotherapy, we developed a novel 15-DNA repair gene signature (DRGS) and its related scoring system and evaluated the efficiency of DRGS in discriminating different molecular and immune characteristics and therapeutic outcomes of gastric adenocarcinoma. The results showed that DRGS high score patients showed significantly better therapeutic outcomes compared to DRGS low score patients (P < 0.001). Integrated analysis of multi-omics data demonstrated that the patients with high DRGS score were characteristic of high levels of anti-tumor lymphocytes infiltration, tumor mutation burden (TMB) and PD-L1 expression, and these patients exhibited a longer overall survival and may benefit more from ICB therapy, as compared to the low-score patients. Therefore, the DRGS and its scoring system may have implications in tailoring immunotherapy in gastric cancers.
- Published
- 2021
44. Discovery and evaluation of cytisine N-isoflavones as novel EGFR/HER2 dual inhibitors
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Yanqing, Wang, Xiaoying, Yin, Lingyan, Chen, Zhixiang, Yin, and Zhicheng, Zuo
- Subjects
Receptor, ErbB-2 ,Organic Chemistry ,Antineoplastic Agents ,Azocines ,Isoflavones ,Biochemistry ,ErbB Receptors ,Alkaloids ,Cell Line, Tumor ,Drug Discovery ,Humans ,Protein Kinase Inhibitors ,Molecular Biology ,Quinolizines ,Cell Proliferation - Abstract
Aberrant signaling of EGFR (ErbB) family members, in particular epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2), is associated with the occurrence and development of many types of human malignancies (e.g., breast, lung, and gastric cancers), and dual targeting of EGFR/HER2 by small-molecular inhibitors has proven to be an effective therapeutic approach for treating these cancers. Herein we extracted and isolated from the medicinal plant Sophora alopecuroides L. a new natural product, dubbed Cytisine N-methylene-(4',7-dihydroxy-3'-methoxy)-isoflavone (CNI1) that features a unique molecular framework. Our biochemical kinase assay suggested that one of its derivative CNI3 exhibited the best, micromolar (μM) inhibition activities against the EGFR (IC
- Published
- 2022
45. Consequences of LED Lights on Root Morphological Traits and Compounds Accumulation in Sarcandra glabra Seedlings
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Chengcheng Zhou, Ke Ren, Jundong Rong, Tianyou He, Muhammad Waqqas Khan Tarin, Lingyan Chen, Jiayi Wan, Yushan Zheng, Dejin Xie, and Deming Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,QH301-705.5 ,phenylpropanoid-derived ,Color ,Secondary Metabolism ,01 natural sciences ,Plant Roots ,Article ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Magnoliopsida ,Chlorogenic acid ,Glucoside ,terpenoids ,Caffeic acid ,Sarcandra glabra ,Food science ,Biology (General) ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,Spectroscopy ,LED light ,Terpenes ,Organic Chemistry ,General Medicine ,transcriptomic ,root tissue ,Quercitrin ,Terpenoid ,Computer Science Applications ,Chemistry ,030104 developmental biology ,chemistry ,Seedlings ,Fraxetin ,Quercetin ,010606 plant biology & botany ,metabolomic - Abstract
This study evaluated the effects of different light spectra (white light, WL, blue light, BL and red light, RL) on the root morphological traits and metabolites accumulation and biosynthesis in Sarcandra glabra. We performed transcriptomic and metabolomic profiling by RNA-seq and ultra-performance liquid chromatography−electrospray ionization−tandem mass spectrometry (UPLC-ESI-MS/MS), respectively. When morphological features were compared to WL, BL substantially increased under-ground fresh weight, root length, root surface area, and root volume, while RL inhibited these indices. A total of 433 metabolites were identified, of which 40, 18, and 68 compounds differentially accumulated in roots under WL (WG) vs. roots under BL (BG), WG vs. roots under RL (RG), and RG vs. BG, respectively. In addition, the contents of sinapyl alcohol, sinapic acid, fraxetin, and 6-methylcoumarin decreased significantly in BG and RG. In contrast, chlorogenic acid, rosmarinyl glucoside, quercitrin and quercetin were increased considerably in BG. Furthermore, the contents of eight terpenoids compounds significantly reduced in BG. Following transcriptomic profiling, several key genes related to biosynthesis of phenylpropanoid-derived and terpenoids metabolites were differentially expressed, such as caffeic acid 3-O-methyltransferase) (COMT), hydroxycinnamoyl-CoA shikimate hydroxycinnamoyl transferase (HCT), O-methyltransferase (OMT), and 1-deoxy-D-xylulose-5-phosphate synthetase (DXS). In summary, our findings showed that BL was suitable for growth and accumulation of bioactive metabolites in root tissue of S. glabra. Exposure to a higher ratio of BL might have the potential to improve the production and quality of S. glabra seedlings, but this needs to be confirmed further.
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- 2021
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46. High-Fat Diet Consumption in Adolescence Induces Emotional Behavior Alterations and Hippocampal Neurogenesis Deficits Accompanied by Excessive Microglial Activation
- Author
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Xiuting Yao, Chenxi Yang, Conghui Wang, Hong Li, Jingyi Zhao, Xiaomin Kang, Zhuodong Liu, Lingyan Chen, Xinyu Chen, Tianshu Pu, Qinyang Li, and Lijie Liu
- Subjects
high-fat diet ,adolescence ,hippocampal neurogenesis ,microglia ,depression ,anxiety ,Neurogenesis ,Emotions ,Organic Chemistry ,General Medicine ,Diet, High-Fat ,Hippocampus ,Catalysis ,Computer Science Applications ,Mice, Inbred C57BL ,Inorganic Chemistry ,Mice ,Animals ,Humans ,Microglia ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy - Abstract
Adolescence is a developmental epoch characterized by massive neural circuit remodeling; thus, the brain is particularly vulnerable to environmental influences during this period. Excessive high-fat diet (HFD) consumption, which is very common among adolescents, has long been recognized as a potent risk factor for multiple mood disorders, including depression and anxiety. However, the precise mechanisms underlying the influences of HFD consumption in adolescence on emotional health are far from clear. In the present study, C57BL/6 mice were fed a control diet (CD) or HFD for about 4 weeks from postnatal day (P) 28 to P60, spanning most of the adolescence period, and then subjected to behavioral assessments and histological examinations. HFD mice exhibited elevated levels of depression and anxiety, decreased hippocampal neurogenesis, and excessive microglial activation in the ventral hippocampus. Furthermore, in HFD-fed mice, microglia showed increased DCX+ inclusions, suggesting aberrant microglial engulfment of newborn neurons in HFD-fed adolescents. To our knowledge, this is the first observation suggesting that the negative effects of HFD consumption in adolescence on emotion and neuroplasticity may be attributed at least in part to aberrant microglial engulfment of nascent neurons, extending our understanding of the mechanism underlying HFD-related affective disorders in young people.
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- 2022
47. Response of Soil Fungal Diversity and Community Composition to Varying Levels of Bamboo Biochar in Red Soils
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Muhammad Waqqas Khan Tarin, Jundong Rong, Dejin Xie, Lingyan Chen, Lili Fan, Muhammad Tayyab, Muhammad Atif Muneer, and Yushan Zheng
- Subjects
Microbiology (medical) ,Bamboo ,QH301-705.5 ,forest management ,Biology ,fungal communities ,Microbiology ,complex mixtures ,Article ,Glomeromycota ,03 medical and health sciences ,Nutrient ,bamboo biochar ,ecological functioning ,soil characteristics ,Virology ,Soil pH ,Biochar ,Biology (General) ,030304 developmental biology ,0303 health sciences ,04 agricultural and veterinary sciences ,biology.organism_classification ,Agronomy ,Soil water ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Species richness ,Red soil - Abstract
Soil fungi play a vital role in soil nutrient dynamics, but knowledge of their diversity and community composition in response to biochar addition into red soil is either limited or inconsistent. Therefore, we determined the impact of bamboo biochar (BB) with increasing concentrations (0, 5, 20, and 80 g kg−1 of soil, referred to as B0, BB5, BB20, and BB80, respectively) on soil physicochemical properties and fungal communities (Illumina high-throughput sequencing) in red soil under Fokenia hodginsii (Fujian cypress). We found that increasing BB levels effectively raised the soil pH and soil nutrients, particularly under BB80. BB addition significantly increased the relative abundance of important genera, i.e., Basidiomycota, Mucoromycota, and Chytridiomycota that could play a key role in ecological functioning, e.g., wood degradation and litter decomposition, improvement in plant nutrients uptake, and resistance to several abiotic stress factors. Soil amended with BB exhibited a substantial ability to increase the fungal richness and diversity; BB80 > BB20 > BB5 > B0. Basidiomycota, Mucoromycota, Glomeromycota, Rozellomycota, Aphelidiomycota, Kickxellomycota, and Planctomycetes were positively associated with soil pH, total nitrogen, phosphorous, and carbon, and available potassium and phosphorous. Besides, the correlation analysis between the soil fungal communities and soil properties also showed that soil pH was the most influential factor in shaping the soil fungal communities in the red soil. These findings have significant implications for a comprehensive understanding of how to ameliorate acidic soils with BB addition, as well as for future research on sustainable forest management, which might increase soil fungi richness, diversity, and functionality in acidic soils.
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- 2021
48. SARS-CoV-2 receptor ACE2 identifies immuno-hot tumors in breast cancer
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Lingyan Chen, Tao Ma, Yan Zhang, Jie Mei, Yun Cai, Tianshu Gao, Rui Xu, Xinqian Yu, Yichao Zhu, and Fei Gao
- Subjects
Mutation ,Chemotherapy ,Tumor microenvironment ,business.industry ,medicine.medical_treatment ,Immunotherapy ,medicine.disease_cause ,medicine.disease ,Transcriptome ,Immune system ,Breast cancer ,medicine ,Cancer research ,Biomarker (medicine) ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
Angiotensin-converting enzyme 2 (ACE2) is known as a host cell receptor for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which is identified to be dysregulated in multiple tumors. Although the characterization of abnormal ACE2 expression in malignancies has been preliminarily explored, in-depth analysis of ACE2 in breast cancer (BRCA) has not been elucidated. A systematic pan-cancer analysis was conducted to assess the expression pattern and immunological role of ACE2 based on RNA-sequencing (RNA-seq) data downloaded from The Cancer Genome Atlas (TCGA). Next, correlations between ACE2 expression immunological characteristics in the BRCA tumor microenvironment (TME) were evaluated. Also, the role of ACE2 in predicting the clinical features and the response to therapeutic options in BRCA was estimated. These findings were subsequently validated in another public transcriptomic cohort as well as a recruited cohort. ACE2 was lowly expressed in most cancers compared with adjacent tissues. ACE2 was positively correlated with immunomodulators, tumor-infiltrating immune cells (TIICs), cancer immunity cycles, immune checkpoints, and tumor mutation burden (TMB). Besides, high ACE2 levels indicated the triple-negative breast cancer (TNBC) subtype of BRCA, lower response to endocrine therapy and higher response to chemotherapy, anti-ERBB therapy, antiangiogenic therapy and immunotherapy. To sum up, ACE2 correlates with an inflamed TME and identifies immuno-hot tumors, which may be used as an auxiliary biomarker for the identification of immunological characteristics in BRCA.
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- 2021
49. Age-Related Changes in the Composition of Intestinal Microbiota in Elderly Chinese Individuals
- Author
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Jiayu Wu, Weiying Ren, Lingyan Chen, Yi Lou, Chu Liu, Yanmei Huang, and Yu Hu
- Subjects
Aged, 80 and over ,Inflammation ,Aging ,China ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Interleukin-8 ,Humans ,Geriatrics and Gerontology ,Middle Aged ,Aged ,Gastrointestinal Microbiome - Abstract
Introduction: Intestinal microbiota affects human health and aging. The composition of intestinal microbiota and inflammation indices in elderly Chinese, especially centenarians, is unclear. Objective: This study aimed to explore the relationships between intestinal microbiota and inflammation in healthy housebound elders in Shanghai, China. Methods: We enrolled 156 differently aged adults and assigned them into 4 groups: those aged 35–64 years were assigned into Group AD; 65–79 years into Group YO; 80–94 years into Group MO; and 95–102 years into Group VO. Results: The diversity of intestinal microbiota in Group VO was significantly reduced compared with that of the other 3 groups. Bacteroidetes abundance in Group VO was significantly lower than that in Groups AD, YO, or MO; Proteobacteria abundance showed the opposite trend. Akkermansia, Bifidobacterium, and Lactobacillus abundance in Group VO was significantly higher than that in the other 3 groups; Anaerostipes, Butyricicoccus, and Faecalibacterium abundance showed the opposite trend. Solobacterium abundance in Group VO was significantly lower than that in the other 3 groups; Campylobacter, Porphyromonas, Escherichia, and Pseudomonas abundance showed the opposite trend. Plasma levels of tumor necrosis factor-α (TNF-α), IL-6, and IL-8 in Group VO were significantly higher than those in Groups AD, YO, and MO, while those in Group MO were significantly higher than those in Groups AD and YO. IL-1β and IL-10 plasma levels were not significantly different among the 4 groups. Proteobacteria abundance was positively correlated with TNF-α and IL-8 levels, while Campylobacter abundance was positively correlated with those of TNF-α and IL-6. Anaerostipes and Faecalibacterium abundance was negatively correlated with TNF-α and IL-6 levels. Conclusions: The diversity of intestinal microbiota in the oldest participants (centenarians) decreased significantly, with several beneficial bacterial strains showing increased or decreased abundance; harmful bacterial species showed a similar trend. Our oldest participants (centenarians) demonstrated significantly increased levels of pro-inflammatory cytokines, which may be related to inflammaging.
- Published
- 2021
50. Various distances and orientations influenced the branching traits of Dendrocalamus minor var. amoenus in Dongshan Island, China
- Author
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Xiaolong Hong, Min Li, Muhammad Waqqas Khan Tarin, Lingyan Chen, Denghui Jiang, Meng Zhang, Tianyou He, Junming Zheng, and Yushan Zheng
- Subjects
0106 biological sciences ,Phenotypic plasticity ,biology ,Ecology ,Coastal pressure ,010604 marine biology & hydrobiology ,Orientations ,Crown (botany) ,biology.organism_classification ,Windbreak ,010603 evolutionary biology ,01 natural sciences ,Dendrocalamus ,Branch traits ,Branch angle ,Branching (linguistics) ,Seashore ,Forest ecology ,Allometry ,Ecology, Evolution, Behavior and Systematics ,QH540-549.5 ,Nature and Landscape Conservation - Abstract
Branch patterns play a key role in assessing plant architecture and are adapted to forest ecosystems. The study aimed to reveal the pattern of branch traits at various distances and orientations from the seashore. For that reason, four sites with various distances from the seashore (SP1: 50–60 m, SP2: 60–100 m, SP3: 100–200 m, and SP4: 200–800 m) were selected and branch traits of Dendrocalamus minor var. amoenus were determined in Dongshan Island, China. The results showed that there were differences in the branch traits between both the 1st-order and 2nd-order branches, and distance from seashore had a significant impact on branch angle, length, diameter, and the number of leaves of 1st-order branches, whereas branch diameter, length, and the number of leaves of 2nd-order branches. In different orientations, the branch angle, length, number, and diameters of the 1st-order and 2nd-order branches showed no significant differences. Nonetheless, the number of leaves of the 1st-order branches were significantly influenced by different orientations. D. minor var. amoenus exhibited strong phenotypic plasticity with longer branches and formed a sparse crown shape. It adjusted the fractal dimension of the crown and branches by modulating the allometric growth relationships among branching traits to adapt to harsh coastal pressure. This study would provide valuable recommendations for D. minor var. amoenus as windbreaks in the coastal climate.
- Published
- 2021
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