604 results on '"K. Sakata"'
Search Results
2. Case study of Geotextile Method on extremely soft ground
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K. Iwataki, K. Zen, K. Sakata, H. Yoshida, N. Kitayama, and T. Fujii
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- 2023
3. Characteristics of Pleural Coccidioidomycosis, a Single Center Experience
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A.A. Abdalla, N. Ashcherkin, and K. Sakata
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- 2023
4. Sakata et al. Fish SedDNA Extraction Protocol v1
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Masayuki K. Sakata
- Abstract
Variations of this standardised protocol have been used by Masayuki K. Sakata and colleagues to successfully extract fish eDNA from modern and historic Japanese lake and river sediments. The method has been used to recover fish species composition data from modern surface sediments from a lake (Sakata et al., 2020) and a small, natural river (Sakata et al., 2021). It has also been applied to detect target fish species in lake sediments up to 100 years old (Sakata et al., 2022). This extraction method represents a consolidation of the methods applied in the following publications: Sakata, M. K., Yamamoto, S., Gotoh, R. O., Miya, M., Yamanaka, H., & Minamoto, T. (2020). Sedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNA. Environmental DNA, 2(4), 505– 518. https://doi.org/10.1002/edn3.75 Sakata, M. K., Watanabe, T., Maki, N., Ikeda, K., Kosuge, T., Okada, H., … Minamoto, T. (2021). Determining an effective sampling method for eDNA metabarcoding: a case study for fish biodiversity monitoring in a small, natural river. Limnology, 22(2), 221–235. https://doi.org/10.1007/s10201-020-00645-9 Sakata, M.K., Tsugeki, N., Kuwae, M., Ochi, N., Hayami, K., Osawa, R., Morimoto, T., Yasashimoto, T., Takeshita, D., Doi, H., & Minamoto, T. (2022). Fish environmental DNA in lake sediment overcomes the gap of reconstructing past fauna in lake ecosystems. bioRxiv, https://doi.org/10.1101/2022.06.16.496507
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- 2023
5. Slower growth of farmed eels stocked into rivers with higher wild eel density
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Ryoshiro Wakiya, Hikaru Itakura, Tatsumu Hirae, Tadamitsu Igari, Miyuki Manabe, Noriaki Matsuya, Katsushi Miyata, Masayuki K. Sakata, Toshifumi Minamoto, Takashi Yada, and Kenzo Kaifu
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Rivers ,Animals ,Fresh Water ,Aquatic Science ,Anguilla ,Ecology, Evolution, Behavior and Systematics - Abstract
Farmed anguillid eels are frequently stocked into natural fresh waters to enhance eel resources, but little is known about what happens to these eels or their interactions with wild eels after stocking. A recent study observed a depressed survival and growth rate of farmed Japanese eels when they were reared with wild eels, which indicated that wild eels might interfere with the survival and growth of farmed-and-stocked eels through intraspecific competition. To contribute to improving eel stocking efficiency, the growth of farmed-and-stocked Japanese eels was compared among four rivers with different wild eel densities using mark-and-recapture studies. Based on the 2-year recapture survey after stocking, it was found that the density of the farmed-and-stocked eels was not significantly different among rivers. The daily growth rates of farmed-and-stocked eels in the rivers with lower wild eel density were significantly higher than those of the eels stocked into the rivers with higher wild eel density. The farmed-and-stocked eels moved significantly greater distances downstream than wild eels that showed sedentary behaviour. This and previous studies indicate that significant questions remain about the effectiveness of stocking farmed eels into water bodies where naturally recruited wild eels are present.
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- 2022
6. Complications of Endobronchial Valve Placement for Bronchoscopic Lung Volume Reduction
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See-Wei Low, Karen L. Swanson, Justin Z. Lee, Min-Choon Tan, Rodrigo Cartin-Ceba, Kenneth K. Sakata, and Fabien Maldonado
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Pulmonary and Respiratory Medicine ,Pulmonary Emphysema ,United States Food and Drug Administration ,Bronchoscopy ,Humans ,Pneumothorax ,Pneumonectomy ,United States - Abstract
Patients with advanced emphysema experience breathlessness due to impaired respiratory mechanics and diaphragm dysfunction. Bronchoscopic lung volume reduction (BLVR) is a minimally invasive bronchoscopic procedure done to reduce hyperinflation and air trapping, promoting atelectasis in the targeted lobe and allowing improved respiratory mechanics. Real-world data on safety and complications outside of clinical trials of BLVR are limited.We queried the US Food and Drug Administrations (FDA) Manufacturers and User Device Experience database from May 2019 to June 2020 for reports involving BLVR with endobronchial valve (EBV) placement. Events were reviewed for data analysis.We identified 124 cases of complications during BLVR with EBV implantation. The most-reported complication was pneumothorax (110/124, 89%), all of which required chest tube placement. A total of 54 of these cases (54/110, 49%) were complicated by persistent air leak requiring additional interventions. Repeat bronchoscopy was needed to remove the valves in 28 patients, 12 were discharged with a Heimlich valve, and 10 had an additional pleural catheter placed. The other complications of BLVR with EBV placement included respiratory failure (6/124, 5%), pneumonia (4/124, 3%), hemoptysis (2/124, 1.6%), valve migration (1/124, 1%), and pleural effusion (1/124, 1%). A total of 14 deaths were reported during that year.Pneumothorax is the most-reported complication for BLVR with EBV placement, and in 65% of cases, pneumothorax is managed without removing valves. Importantly, 14 deaths were reported during that timeframe. Further studies are needed to estimate the true magnitude of the complications associated with BLVR.
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- 2022
7. JUMP Cell Painting dataset: morphological impact of 136,000 chemical and genetic perturbations
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Srinivas Niranj Chandrasekaran, Jeanelle Ackerman, Eric Alix, D. Michael Ando, John Arevalo, Melissa Bennion, Nicolas Boisseau, Adriana Borowa, Justin D. Boyd, Laurent Brino, Patrick J. Byrne, Hugo Ceulemans, Carolyn Ch’ng, Beth A. Cimini, Djork-Arne Clevert, Nicole Deflaux, John G Doench, Thierry Dorval, Regis Doyonnas, Vincenza Dragone, Ola Engkvist, Patrick W. Faloon, Briana Fritchman, Florian Fuchs, Sakshi Garg, Tamara J. Gilbert, David Glazer, David Gnutt, Amy Goodale, Jeremy Grignard, Judith Guenther, Yu Han, Zahra Hanifehlou, Santosh Hariharan, Desiree Hernandez, Shane R Horman, Gisela Hormel, Michael Huntley, Ilknur Icke, Makiyo Iida, Christina B. Jacob, Steffen Jaensch, Jawahar Khetan, Maria Kost-Alimova, Tomasz Krawiec, Daniel Kuhn, Charles-Hugues Lardeau, Amanda Lembke, Francis Lin, Kevin D. Little, Kenneth R. Lofstrom, Sofia Lotfi, David J. Logan, Yi Luo, Franck Madoux, Paula A. Marin Zapata, Brittany A. Marion, Glynn Martin, Nicola Jane McCarthy, Lewis Mervin, Lisa Miller, Haseeb Mohamed, Tiziana Monteverde, Elizabeth Mouchet, Barbara Nicke, Arnaud Ogier, Anne-Laure Ong, Marc Osterland, Magdalena Otrocka, Pieter J. Peeters, James Pilling, Stefan Prechtl, Chen Qian, Krzysztof Rataj, David E Root, Sylvie K. Sakata, Simon Scrace, Hajime Shimizu, David Simon, Peter Sommer, Craig Spruiell, Iffat Sumia, Susanne E Swalley, Hiroki Terauchi, Amandine Thibaudeau, Amy Unruh, Jelle Van de Waeter, Michiel Van Dyck, Carlo van Staden, Michał Warchoł, Erin Weisbart, Amélie Weiss, Nicolas Wiest-Daessle, Guy Williams, Shan Yu, Bolek Zapiec, Marek Żyła, Shantanu Singh, and Anne E. Carpenter
- Abstract
Image-based profiling has emerged as a powerful technology for various steps in basic biological and pharmaceutical discovery, but the community has lacked a large, public reference set of data from chemical and genetic perturbations. Here we present data generated by the Joint Undertaking for Morphological Profiling (JUMP)-Cell Painting Consortium, a collaboration between 10 pharmaceutical companies, six supporting technology companies, and two non-profit partners. When completed, the dataset will contain images and profiles from the Cell Painting assay for over 116,750 unique compounds, over-expression of 12,602 genes, and knockout of 7,975 genes using CRISPR-Cas9, all in human osteosarcoma cells (U2OS). The dataset is estimated to be 115 TB in size and capturing 1.6 billion cells and their single-cell profiles. File quality control and upload is underway and will be completed over the coming months at the Cell Painting Gallery:https://registry.opendata.aws/cellpainting-gallery. A portal to visualize a subset of the data is available athttps://phenaid.ardigen.com/jumpcpexplorer/.
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- 2023
8. Universal performance of benzalkonium chloride for the preservation of environmental DNA in seawater samples
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Reiji Masuda, Masayuki K. Sakata, Toshiaki Jo, Toshifumi Minamoto, and Hiroaki Murakami
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Benzalkonium chloride ,Environmental chemistry ,medicine ,Environmental science ,Ocean Engineering ,Environmental DNA ,Seawater ,medicine.drug - Abstract
Environmental DNA (eDNA) analysis allows noninvasive and cost-effective monitoring of macroorganisms' distribution and composition in aquatic ecosystems. Benzalkonium chloride (BAC) is an inexpensive and simple preservative for eDNA in water samples and has been used in many eDNA studies for preventing its degradation during transportation. However, its preservation performance has limitedly been evaluated by species-specific assays, targeting short fragments of mitochondrial DNA in freshwater and brackish ecosystems. Here, we examined the performance of BAC in preserving eDNA in seawater samples, targeting different fragment lengths of mitochondrial and nuclear eDNA, and community information inferred by eDNA metabarcoding. We quantified the time series changes of Japanese jack mackerel (Trachurus japonicus) eDNA concentrations in experimental tanks and inshore seawater to compare the yields and decay rates of eDNA between BAC treatments. For both tank and field samples, BAC treatment substantially suppressed the degradation of all types of target eDNA and increased the eDNA yields at the start of the experiment. In addition, we performed eDNA metabarcoding targeting fish community to compare the species richness and composition in seawater samples between BAC treatments, showing that the number of fish species in field samples hardly varied throughout a day by BAC treatment. Our findings indicate high versatility of BAC in preserving both the quantitative (copy number) and the qualitative (species richness) information on various types of aqueous eDNA in various environmental conditions. BAC should therefore be used to minimize the false-negative detection of eDNA, regardless of target genetic regions, fragment sizes, environmental conditions, and detection strategies.
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- 2021
9. Utility of in vivo zebrafish cardiac assay to predict the functional impact of KCNQ1 variants
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S Cui, K Hayashi, K Usuda, S Usui, K Sakata, M Kawashiri, T Kusayama, T Tsuda, N Fujino, T Kato, and M Takamura
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Cardiology and Cardiovascular Medicine - Abstract
Background Genetic testing for inherited arrhythmias and discriminating pathogenic from benign variants are integral for the gene-based medicine. However, the high throughput in vivo functional analysis for the rare variants of the KCNQ1 potassium channel is scarce. Purpose We tested the utility of the in vivo zebrafish cardiac assay for determining the pathogenicity of the KCNQ1 variants identified in patients with long QT syndrome (LQTS) and atrial fibrillation (AF). Methods We generated a knock-out zebrafish with CRISPR-mediated insertions or deletions of the KCNQ1 homolog in zebrafish (kcnq1+/+). To test the utility of the cardiac assay, we used five KCNQ1 variants identified in patients with LQTS or familial AF. Human wild-type or mutant KCNQ1 cRNA (Q1) was co-injected with human KCNE1 cRNA (E1) into the F3 generation embryos with homozygous deletions. We dissected the hearts from the thorax at 72 hour-post-fertilization and measured transmembrane potential in zebrafish heart using the disrupted patch technique. Action potential duration was calculated as the time interval between the peak maximum upstroke velocity and 90% of repolarization (APD90). We compared the APD90s with patients' clinical phenotype and IKs density measured by patch-clamp technique in heterologous system. Results The mean APD90 of embryos with kcnq1del/del was 279±48 ms, which was restored by injecting Q1 WT and E1 (159±29 ms) to that with kcnq1+/+ (167±28 ms). We tested if the mean APD90 of embryos with kcnq1del/del was restored (shortened) by injecting the KCNQ1 variants. First we tested the dominant negative variant p.S277L and the trafficking deficient variant p.T587M. Patients with these variants showed significant prolonged QT intervals, and patch clamp study showed both variants caused the non-functional channels. Zebrafish cardiac assay showed the mean APD90 of embryos with kcnq1del/del+ Q1 S277L+E1 or Q1 T587M+E1 was significantly longer than that with kcnq1del/del+Q1 WT+E1 (Table). Next we tested in-frame variant c.1472_1473 ins GGACCT, which was identified from a patient with AF and normal QT interval. Patch clamp study showed the current density of the mutant KCNQ1 channel with KCNE1 was comparable to that of wild-type KCNQ1 channel with KCNE1. Zebrafish assay showed the mean APD90 of embryos with kcnq1del/del shortened by injecting Q1 insACCTGG +E1 (Table). Finally we tested a missense variant p.R451Q, which was identified from a patient with LQTS. Patch clamp study showed the currents in the cells transfected with R451Q+KCNE1 were similar to those with WT+KCNE1. Zebrafish assay showed the mean APD90 of embryos with kcnq1del/del+Q1 R451Q+E1 was longer than that with kcnq1del/del+Q1 WT+E1 (Table). Conclusions Functional analysis of in vivo zebrafish cardiac assay might be useful for determining the pathogenicity of rare variants in patients with LQTS. Funding Acknowledgement Type of funding sources: Public Institution(s). Main funding source(s): The Grant-in-Aid for Scientific Research (C)
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- 2022
10. Application of environmental DNA metabarcoding in a lake with extensive algal blooms
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Toshifumi Minamoto, Qianqian Wu, Masayuki K. Sakata, Hiroki Yamanaka, and Deyi Wu
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Ecology ,biology ,Aquatic ecosystem ,fungi ,Biodiversity ,Aquatic Science ,biology.organism_classification ,Algal bloom ,Environmental DNA metabarcoding analysis ,Lake Taihu ,Fish species composition ,Freshwater fish ,Algal blooms ,%22">Fish ,Environmental science ,Environmental DNA ,Ecosystem ,Water Science and Technology ,Global biodiversity - Abstract
Recently, environmental DNA (eDNA) metabarcoding techniques have been applied to biodiversity investigations in aquatic ecosystems. However, no study has yet tested whether this technique is effective for water bodies in which extensive algal blooms break out. In this study, fish eDNA metabarcoding was carried out in Lake Taihu, which experiences extensive algal blooms, to confirm whether the technique is also effective for fish diversity research in ecosystems with frequent and extensive blooms. In December 2016, three samples were collected, including one collected in the presence of algal blooms and two collected in the absence of algal blooms. In August 2017, six samples were collected, including three collected in the presence of algal blooms and three in the absence of algal blooms. Equal amount of water samples (1 L) was collected from each site; however, the actual amount of filtrate varied with the site. Twenty-seven freshwater fish species were detected from the water samples collected in Lake Taihu. The results showed that the composition of the detected species did not differ whether or not blooms were present. However, the amount of filtration could influence the number of species detected. The results suggest that future eDNA metabarcoding studies under similar water environments should increase the amount of filtration to maximize number of species detected.
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- 2021
11. Robotic-assisted Bronchoscopy to Diagnose Malakoplakia
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Jee H. Kim, Kenneth K. Sakata, Maxwell Smith, and Natalya Azadeh
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- 2022
12. May 2021 Imaging Case of the Month: A Growing Indeterminate Solitary Nodule
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Joseph Jeehoon Kim, MD, Kenneth K. Sakata, MD, Natalya Azadeh, MD, MPH, Maxwell Smith, MD, and Michael B. Gotway, MD
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lung biopsy ,malakoplakia ,nodule ,R5-130.5 ,RC86-88.9 ,solitary nodule ,michaelis-gutmann bodies ,pulmonary nodule ,Medical emergencies. Critical care. Intensive care. First aid ,cardiac transplantation ,lung nodule ,General works ,heart transplant ,immunocompromised host - Abstract
No abstract available. Article truncated after 150 words. A 58-year-old woman with a history of orthotopic heart transplant, performed for Adriamycin-induced cardiomyopathy, treated with mycophenolate and tacrolimus, presented for routine interval follow up. The patient’s past medical history was significant for follicular thyroid carcinoma treated with total thyroidectomy and bilateral breast carcinoma in remission as well as hypothyroidism and type II diabetes mellitus. In addition to tacrolimus and mycophenolate, the patient’s medications included aspirin, insulin, itraconazole (for anti-fungal prophylaxis), levothyroxine, prednisone (tapering since transplant), and valganciclovir. The patient recently complained of rhinorrhea and cough productive of brown-tinged sputum, improving over the previous 2 weeks; she denied fever, chills, shortness of breath, night sweats chest pain, or gastrointestinal symptoms. Physical examination showed the patient to be afebrile with normal heart and respiratory rates and blood pressure. Her room air oxygen saturation was 99%. The patient’s complete blood count and serum chemistries showed largely normal values, with the white blood …
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- 2021
13. PNEUMOMEDIASTINUM AND PNEUMOTHORAX IN COVID-19 PNEUMONIA: A MATCHED CASE-CONTROL STUDY
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ANUSHA DEVARAJAN, AYSUN TEKIN, KENNETH K SAKATA, RODRIGO CARTIN-CEBA, MAYANK SHARMA, SHAHRAZ QAMAR, DIANA J VALENCIA MORALES, STEPHANIE WELLE, NIKHIL SHARMA, JAMIL TAJI, SANDEEP KHOSA, AMOS LAL, MEGHAN L BROWN, FAHIMEH TALAEI, JUAN PABLO DOMECQ, and NATALYA AZADEH
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Pulmonary and Respiratory Medicine ,Cardiology and Cardiovascular Medicine ,Critical Care and Intensive Care Medicine - Published
- 2022
14. Fish environmental DNA in lake sediment overcomes the gap of reconstructing past fauna in lake ecosystems
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Masayuki K. Sakata, Narumi Tsugeki, Michinobu Kuwae, Natsuki Ochi, Kana Hayami, Ryohei Osawa, Teppei Morimoto, Tetsu Yasashimoto, Daiki Takeshita, Hideyuki Doi, and Toshifumi Minamoto
- Abstract
Underwater sediments are a natural archive of biological information. Reconstruction of past fauna has been conducted for various taxonomic groups using morphological remains and DNA derived from these remains. However, information on past occurrences of fish species, the top predator of lake ecosystems, could have been reproduced only in exceptional environments, and past quantitative information on fish, particularly in lake ecosystems, has been a knowledge gap in reconstructing past fauna. Tracking the quantitative fluctuations of fish is essential for reconstructing multiple trophic levels of organisms in lake ecosystems.To acquire past quantitative fish information from lake sediments, we collected approximately 30 cm-length of underwater sediments in Lake Biwa. We extracted sedimentary environmental DNA (eDNA) and measured temporal fluctuations in the eDNA concentration of the native and fishery target species Plecoglossus altivelis and Gymnogobius isaza. For P. altivelis, we examined the possibility of tracking quantitative fluctuations by comparing sedimentary eDNA with recorded catch per unit effort (CPUE).The chronology of the sediments allowed us to obtain information on sediments collected in Lake Biwa over the past 100 years. The deepest depths at which sedimentary eDNA was detected were 30 and 13 cm for P. altivelis and G. isaza from the surface, corresponding to approximately 100 and 30 years ago, respectively. In the comparison of sedimentary eDNA concentrations and biomass, we found a significant correlation between the CPUE of P. altivelis and its sedimentary eDNA concentration adjusted to compensate for DNA degradation. Sedimentary eDNA fluctuations were observed in P. altivelis, possibly reflecting the abundance fluctuation due to variations in the main food resources of zooplankton.Our findings provide essential pieces for the reconstruction of past fauna of lake ecosystems. The addition of quantitative information on fish species will reach a new phase, for instance, by investigating population shifts or biological interactions in the reconstruction of past fauna in lake ecosystems.
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- 2022
15. CALIPER-Based Lobar Changes Following Bronchoscopic Lung Volume Reduction with Endobronchial Valves: A Multicenter Review
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M. Martin, S. Rajagopalan, B.J. Bartholmai, D. Abia Trujillo, A. Yu Lee-Mateus, S. Fernandez-Bussy, K. Sakata, and T. Peikert
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- 2022
16. Employing Photocatalysis for the Design and Preparation of DNA‐Encoded Libraries: A Case Study
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Dominik K. Kölmel, Jinqiao Wan, Barry A. Morgan, Hongyao Zhu, Mark Edward Flanagan, Sylvie K. Sakata, and Anthony R. Harris
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Personal account ,010405 organic chemistry ,business.industry ,Drug discovery ,General Chemical Engineering ,Design elements and principles ,DNA ,General Chemistry ,Photochemical Processes ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Catalysis ,0104 chemical sciences ,Small Molecule Libraries ,Drug Design ,Materials Chemistry ,Key (cryptography) ,Combinatorial Chemistry Techniques ,Software engineering ,business ,Gene Library - Abstract
This Personal Account describes the authors' foray into DNA-encoded libraries. The article addresses several key aspects of this hit generation technology, from the development of new synthetic methodology to the subsequent conception, design, and delivery of a DNA-encoded library. In particular, we have been engaged in adapting photocatalytic reactions to the idiosyncratic requirements of DNA-encoded chemistry. We have chosen one such methodology, namely a photocatalytic [2+2] cycloaddition reaction, to showcase how we employed property-based computational analyses to guide the selection and validation of building blocks for the production of a library. Ultimately, these novel bond disconnections and design principles led to the assembly of a DNA-encoded library that is composed of structurally diverse compounds within largely desirable property space and, therefore, well positioned to deliver novel chemical matter for drug discovery programs.
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- 2021
17. Rhodium(III)-Catalyzed C–H Activation: Annulation of Petrochemical Feedstocks for the Construction of Isoquinolone Scaffolds
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Dehuan Kong, Wei Li, Ryan L. Patman, Sylvie K. Sakata, Indrawan James Mcalpine, Joyann S. Barber, Nicole Sun, and Sajiv Krishnan Nair
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Annulation ,Ethylene ,010405 organic chemistry ,Chemistry ,Site selectivity ,Organic Chemistry ,chemistry.chemical_element ,010402 general chemistry ,Propyne ,01 natural sciences ,Combinatorial chemistry ,Cycloaddition ,0104 chemical sciences ,Catalysis ,Rhodium ,chemistry.chemical_compound ,Petrochemical - Abstract
We describe a simple and robust procedure for the Rh(III)-catalyzed [4+2] cycloaddition of feedstock gases enabled through C–H activation. A diverse set of 3,4-dihydroisoquinolones and 3-methylisoquinolones have been prepared in good to excellent yields. The effects of using ethylene and propyne as coupling partners on C–H site selectivity have also been explored with a representative set of substrates and are discussed herein.
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- 2021
18. Trends in Intrabronchial Valve Implantation in Patients with Persistent Air Leak: Analysis of a Nationwide Database over a 10-Year Period
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Karen L. Swanson, Laszlo T. Vaszar, See Wei Low, Kenneth K. Sakata, and Rodrigo Cartin-Ceba
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,business.industry ,Chest Tubes ,Emergency medicine ,Nationwide database ,MEDLINE ,Humans ,Pneumothorax ,Medicine ,Persistent air leak ,In patient ,business - Published
- 2020
19. Determining an effective sampling method for eDNA metabarcoding: a case study for fish biodiversity monitoring in a small, natural river
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Kousuke Ikeda, Toshihiro Kosuge, Masayuki K. Sakata, Hiroaki Okada, Nobutaka Maki, Takeshi Watanabe, Hiroki Yamanaka, Toshifumi Minamoto, Tetsuya Sado, and Masaki Miya
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0106 biological sciences ,Shore ,geography ,geography.geographical_feature_category ,Ecology ,010604 marine biology & hydrobiology ,Aquatic ecosystem ,Biodiversity ,Sampling (statistics) ,Sediment ,Aquatic Science ,010603 evolutionary biology ,01 natural sciences ,Natural (archaeology) ,Taxon ,Environmental science ,Environmental DNA ,Water Science and Technology - Abstract
In recent years, biodiversity loss has become one of the most serious environmental issues worldwide, especially in aquatic ecosystems. To avoid diversity loss, it is necessary to monitor biological communities, and environmental DNA (eDNA) metabarcoding has been developed as a rapid, noninvasive, and cost-effective method for aquatic biodiversity monitoring. Although this method has been applied to various environments and taxa, a detailed assessment of the efficient sampling methods for monitoring is still required. In this study, we explored eDNA metabarcoding sampling methods for fish at a single site to maximize the number of detected species using realistic effort in a natural, small river. We considered the following three parameters: sample type (water or sediment), sample position at a site (right and left shore and center of the river), and water volume (10–4000 mL). The results suggested that the number of detected species from sedimentary eDNA was equivalent to that from aqueous eDNA, although the species composition was different. The number of detected species could be saturated by collecting a 1000 mL water sample, regardless of sampling position within a survey site. However, sedimentary eDNA showed a spatially heterogeneous species composition between sampling positions within a survey site despite the short distance (5 m) between positions, without apparent differences in physical properties such as velocity and sediment particle distribution. By completing eDNA biodiversity monitoring of fish with 1000 mL water samples across the whole river, we detected more fish species than in previous traditional surveys conducted at the same sites. Thus, the aqueous eDNA metabarcoding method is as efficient as traditional surveys, while sedimentary eDNA metabarcoding could complement the results of aqueous eDNA metabarcoding.
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- 2020
20. Low-Temperature Reduction of Graphene Oxide Using the HDDR Process for Electrochemical Supercapacitor Applications
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Lusinete Pereira Barbosa, R.N. Faria, Solange K. Sakata, F.G. Benitez Jara, A.J. Peruzzi, Julio César Serafim Casini, and P. D. V. Cruz
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Supercapacitor ,Materials science ,Equivalent series resistance ,Graphene ,Mechanical Engineering ,Oxide ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Capacitance ,Energy storage ,0104 chemical sciences ,law.invention ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,Mechanics of Materials ,law ,Desorption ,Scientific method ,General Materials Science ,0210 nano-technology - Abstract
In the present work, attempts of reducing a graphene oxide powder using a low temperature hydrogenation disproportionation desorption and the recombination process (L-HDDR) has been carried out. A lower processing temperature in large scale production is significant when costs are concerned. Graphite oxide was prepared using a modified Hummers’ method dispersed in ethanol and exfoliated using ultrasonication to produce Graphene Oxide (GO). Investigations have been carried out by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The experimental results of L-HDDR processing graphene oxide powder, using unmixed hydrogen at 400°C and relatively low pressures (
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- 2020
21. Bronchoscopic Cryobiopsy and Forceps Biopsy for the Diagnostic Evaluation of Diffuse Parenchymal Lung Disease in Clinical Practice
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Paul A. Decker, Eunhee S. Yi, Teng Moua, David E. Midthun, Matthew Koslow, Janani S. Reisenauer, Ryan Kern, Kenneth K. Sakata, Darlene R. Nelson, Eric S. Edell, Anja C. Roden, Jay H. Ryu, and John J. Mullon
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medicine.medical_specialty ,TBFB, transbronchial forceps biopsy ,Context (language use) ,030204 cardiovascular system & hematology ,03 medical and health sciences ,Idiopathic pulmonary fibrosis ,0302 clinical medicine ,CTD, connective tissue disease ,Usual interstitial pneumonia ,Biopsy ,medicine ,IPF, idiopathic pulmonary fibrosis ,SLB, surgical lung biopsy ,030212 general & internal medicine ,UIP, usual interstitial pneumonia ,lcsh:R5-920 ,DAH, diffuse alveolar hemorrhage ,medicine.diagnostic_test ,business.industry ,Medical record ,Interstitial lung disease ,Odds ratio ,medicine.disease ,CT, computed tomography ,OR, odds ratio ,HP, hypersensitivity pneumonitis ,Pneumothorax ,TBCB, transbronchial cryobiopsy ,ILD, interstitial lung disease ,Original Article ,Radiology ,BAL, bronchoalveolar lavage ,business ,lcsh:Medicine (General) - Abstract
Objective: To assess the contribution and safety of bronchoscopic cryobiopsy vs traditional forceps biopsy used in clinical practice for diagnosing diffuse parenchymal lung disease (DPLD). Patients and Methods: We identified 271 patients who underwent bronchoscopic biopsy for DPLD at Mayo Clinic, MN (June 1, 2013, through September 30, 2017). Medical records were reviewed including prebiopsy clinical and radiographic impressions. Diagnostic yield was assessed in terms of a specific histologic pattern resulting in a diagnosis when combined with the clinical-radiologic context. Clinical utility was defined as a biopsy result deemed useful in patient management. Results: The cohort included 120 cryobiopsy and 151 forceps biopsy cases with mean age 61±14 years and 143 (53%) men. Diagnostic yield (55% vs 41%; odds ratio [OR], 1.73; 95% CI, 1.07 to 2.83; P=.026) and clinical utility (60% vs 40%; OR, 2.21; 95% CI, 1.36 to 3.63; P=.001) were higher for the cryobiopsy group, and the association remained after control for prebiopsy clinical impressions (OR, 2.21; 95% CI, 1.22 to 4.08; P=.010 and OR, 3.23; 95% CI, 1.76 to 6.10; P
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- 2020
22. Sedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNA
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Hiroki Yamanaka, Masayuki K. Sakata, Satoshi Yamamoto, Toshifumi Minamoto, Ryo O. Gotoh, and Masaki Miya
- Subjects
lcsh:GE1-350 ,decay rate ,Aqueous solution ,Ecology ,Fish species ,Sediment ,sedimentary eDNA ,environmental DNA ,MiFish metabarcoding ,lcsh:Microbial ecology ,sediment ,Environmental chemistry ,Genetics ,Environmental science ,lcsh:QR100-130 ,Composition (visual arts) ,Environmental DNA ,Sedimentary rock ,real‐time PCR ,Ecology, Evolution, Behavior and Systematics ,lcsh:Environmental sciences - Abstract
Aqueous environmental DNA (eDNA) analysis has been applied to the monitoring of various ecosystems and taxa, and the characteristics of aqueous eDNA have been previously studied. In contrast, although sedimentary eDNA has been used to restore past information, the characteristics of sedimentary eDNA are not well understood. In this study, we compared the properties of sedimentary and aqueous eDNA of macro‐organisms. First, to clarify the preservation ability of sediments, we compared the difference in decay rates between aqueous and sedimentary eDNA using samples collected from a biotope (an artificial pond prepared with concrete). Next, to clarify the biological information retained in sedimentary eDNA both qualitatively and quantitatively, we compared eDNA concentrations between sediment and water samples collected simultaneously from a lake, and the fish species detected by eDNA metabarcoding were also compared. The results demonstrated the following: (a) the decay rate (decreased eDNA copy number divided by the initial eDNA copy number per unit time) of sedimentary eDNA (0.00033 ± 0.000049 [mean ± SE]/hr) was lower than that of aqueous eDNA (0.01863 ± 0.0011/hr); (b) sedimentary eDNA concentration of the mitochondrial marker of three fish species was higher than aqueous eDNA concentration for the same sample weight (12.5–1,456.9 times); and (c) the species composition obtained by metabarcoding was not significantly different between sediment and water; however, considering the lower decay rate of sedimentary eDNA, using both sample types may provide more comprehensive information of species distribution. Thus, sedimentary eDNA analysis will expand future biomonitoring and ecological studies by providing a difference in timescale.
- Published
- 2020
23. Feasibility and Efficacy of a Non-Opioid Based Pain Management After Medical Thoracoscopy
- Author
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See-Wei Low, John J. Mullon, Karen L. Swanson, Ryan M. Kern, Darlene R. Nelson, Sebastian Fernandez-Bussy, and Kenneth K. Sakata
- Abstract
Prescription opioids are a major cause of the opioid epidemic. Despite the minimally invasive nature of medical thoracoscopy (MT), data on the efficacy of non-opioid-based pain control after MT is lacking. The purpose of this study is to assess the feasibility and efficacy of a non-opioid-based pain management strategy in patients who underwent MT.We performed a retrospective analysis of all patients who underwent MT in the Mayo Clinic (Minnesota and Arizona) outpatient setting. We assessed their pain level and the need for analgesia post-MT from August 1, 2019, to May 24, 2021.Forty patients were included. In the first 24 hours, 5/40 (12.5%) reported no pain. Twenty-eight patients out of 40 (70%) reported minor pain (pain scale 1-3), and 7/40 (17.5%) reported moderate pain (pain scale 4-6). No patients reported severe pain. Twenty-two out of 35 patients who experienced discomfort (63%) required acetaminophen, 6/35 patients (17%) required nonsteroidal anti-inflammatory drug, and 7/35 patients (20%) did not require analgesia. Of the 7 patients who had moderate pain, 5 (71%) reported that the moderate pain improved to mild at 72 hours post-MT. Zero patients required opioids, and none reported contacting any provider to manage the pain post-MT. Fourteen patients (78%) who had both parietal pleural biopsies and tunneled pleural catheter placed reported minor pain, 3 patients (17%) reported moderate pain, and 1 patient (6%) experienced no discomfort.MT is well-tolerated by patients with non-opioid-based pain management strategy as needed if there is no absolute contraindication.
- Published
- 2022
24. Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia
- Author
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Masayuki K. Sakata, Mone U. Kawata, Atsushi Kurabayashi, Takaki Kurita, Masatoshi Nakamura, Tomoyasu Shirako, Ryosuke Kakehashi, Kanto Nishikawa, Mohamad Yazid Hossman, Takashi Nishijima, Junichi Kabamoto, Masaki Miya, and Toshifumi Minamoto
- Subjects
Insecta ,Arthropoda ,Ceramiales ,Florideophyceae ,universal primer ,Amphibia ,Genetics ,Caraboidea ,Animalia ,Stenolophini ,Chordata ,Plantae ,Molecular Biology ,Nature and Landscape Conservation ,Stenolophus ,Rhodomelaceae ,Аgricultural ecosystem ,Bostrychia ,environmental DNA ,Biota ,Harpalinae ,Rhodophyta ,biodiversity monitoring ,metabarcoding ,Eurhodophytina ,Animal Science and Zoology ,Carabidae - Abstract
Biodiversity monitoring is important for the conservation of natural ecosystems in general, but particularly for amphibians, whose populations are pronouncedly declining. However, amphibians’ ecological traits (e.g. nocturnal or aquatic) often prevent their precise monitoring. Environmental DNA (eDNA) metabarcoding – analysis of extra-organismal DNA released into the environment – allows the easy and effective monitoring of the biodiversity of aquatic organisms. Here, we developed and tested the utility of original PCR primer sets. First, we conducted in vitro PCR amplification tests with universal primer candidates using total DNA extracted from amphibian tissues. Five primer sets successfully amplified the target DNA fragments (partial 16S rRNA gene fragments of 160–311 bp) from all 16 taxa tested (from the three living amphibian orders Anura, Caudata and Gymnophiona). Next, we investigated the taxonomic resolution retrieved using each primer set. The results revealed that the universal primer set “Amph16S” had the highest resolution amongst the tested sets. Finally, we applied Amph16S to the water samples collected in the field and evaluated its detection capability by comparing the species detected using eDNA and physical survey (capture-based sampling and visual survey) in multiple agricultural ecosystems across Japan (160 sites in 10 areas). The eDNA metabarcoding with Amph16S detected twice as many species as the physical surveys (16 vs. 8 species, respectively), indicating the effectiveness of Amph16S in biodiversity monitoring and ecological research for amphibian communities., 水をくむだけの新しい両生類の調査法. 京都大学プレスリリース. 2022-02-22.
- Published
- 2022
25. Effects of sampling seasons and locations on fish environmental DNA metabarcoding in dam reservoirs
- Author
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Hidetaka Ichiyanagi, Takashi Inagawa, Izumi Katano, Masayuki K. Sakata, Hideyuki Doi, Ryo O. Gotoh, Masaki Miya, Katsuki Nakai, Jiro Okitsu, Hirotoshi Sato, Kana Hayami, Hiroki Yamanaka, and Toshifumi Minamoto
- Subjects
0106 biological sciences ,reservoir ,Fauna ,Biodiversity ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,lcsh:QH540-549.5 ,Environmental DNA ,Monitoring methods ,Ecology, Evolution, Behavior and Systematics ,Original Research ,030304 developmental biology ,Nature and Landscape Conservation ,Shore ,fish ,0303 health sciences ,geography ,geography.geographical_feature_category ,Ecology ,Sampling (statistics) ,environmental DNA ,Fishery ,metabarcoding ,Environmental science ,%22">Fish ,lcsh:Ecology ,Global biodiversity - Abstract
Environmental DNA (eDNA) analysis has seen rapid development in the last decade, as a novel biodiversity monitoring method. Previous studies have evaluated optimal strategies, at several experimental steps of eDNA metabarcoding, for the simultaneous detection of fish species. However, optimal sampling strategies, especially the season and the location of water sampling, have not been evaluated thoroughly. To identify optimal sampling seasons and locations, we performed sampling monthly or at two‐monthly intervals throughout the year in three dam reservoirs. Water samples were collected from 15 and nine locations in the Miharu and Okawa dam reservoirs in Fukushima Prefecture, respectively, and five locations in the Sugo dam reservoir in Hyogo Prefecture, Japan. One liter of water was filtered with glass‐fiber filters, and eDNA was extracted. By performing MiFish metabarcoding, we successfully detected a total of 21, 24, and 22 fish species in Miharu, Okawa, and Sugo reservoirs, respectively. From these results, the eDNA metabarcoding method had a similar level of performance compared to conventional long‐term data. Furthermore, it was found to be effective in evaluating entire fish communities. The number of species detected by eDNA survey peaked in May in Miharu and Okawa reservoirs, and in March and June in Sugo reservoir, which corresponds with the breeding seasons of many of fish species inhabiting the reservoirs. In addition, the number of detected species was significantly higher in shore, compared to offshore samples in the Miharu reservoir, and a similar tendency was found in the other two reservoirs. Based on these results, we can conclude that the efficiency of species detection by eDNA metabarcoding could be maximized by collecting water from shore locations during the breeding seasons of the inhabiting fish. These results will contribute in the determination of sampling seasons and locations for fish fauna survey via eDNA metabarcoding, in the future., Number of detected species by eDNA metabarcoding for (a) Miharu dam reservoir, (b) Okawa dam reservoir, and (c) Sugo dam reservoir. The number of species detected by eDNA survey peaked in May in Miharu and Okawa reservoirs, and in March and June in Sugo reservoir, which corresponds with the breeding seasons of many of the fish species inhabiting the reservoirs.
- Published
- 2020
26. Characterization of Specific N-α-Acetyltransferase 50 (Naa50) Inhibitors Identified Using a DNA Encoded Library
- Author
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Paul G. Richardson, Xiaoyun Meng, Karen A. Maegley, A.E. Stewart, Pei-Pei Kung, Jose L Montano, Ya-Li Deng, Jinqiao Wan, Michael R. Gehring, Jordan L. Meier, Brigitte S Naughton, Stephan Grant, Dou Dengfeng, Chakrapani Subramanyam, Anthony R. Harris, Samantha Elizabeth Greasley, Barry A. Morgan, Wen Yan, Xuemin Cheng, Prakash B. Palde, William K Sonnenburg, Junli Feng, Chen Qiuxia, Thomas A Paul, Gary M. Gallego, Sylvie K. Sakata, Sergei Timofeevski, Patrick Bingham, Benjamin J. Burke, and Alex Shaginian
- Subjects
chemistry.chemical_classification ,010405 organic chemistry ,Chemistry ,Stereochemistry ,Organic Chemistry ,Target engagement ,Substrate (chemistry) ,01 natural sciences ,Biochemistry ,Cocrystal ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Enzyme ,Mechanism of action ,Acetyltransferase ,Drug Discovery ,medicine ,A-DNA ,medicine.symptom ,Selectivity - Abstract
[Image: see text] Two novel compounds were identified as Naa50 binders/inhibitors using DNA-encoded technology screening. Biophysical and biochemical data as well as cocrystal structures were obtained for both compounds (3a and 4a) to understand their mechanism of action. These data were also used to rationalize the binding affinity differences observed between the two compounds and a MLGP peptide-containing substrate. Cellular target engagement experiments further confirm the Naa50 binding of 4a and demonstrate its selectivity toward related enzymes (Naa10 and Naa60). Additional analogs of inhibitor 4a were also evaluated to study the binding mode observed in the cocrystal structures.
- Published
- 2020
27. Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt ( Spirinchus lanceolatus )
- Author
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Hitoshi Araki, Hisaya Nii, Satoko Namba, Tetsu Yatsuyanagi, Takashi Kanbe, Hiroki Mizumoto, Shoko Kamada, Toshifumi Minamoto, Ryotaro Ishida, Masayuki K. Sakata, and Yumi Kobayashi
- Subjects
lcsh:GE1-350 ,Fish migration ,Ecology ,Spirinchus lanceolatus ,endemic species ,Zoology ,anadromous fish ,Biology ,Shishamo ,environmental DNA ,biology.organism_classification ,lcsh:Microbial ecology ,reproductive migration ,biomonitoring ,Biomonitoring ,Genetics ,lcsh:QR100-130 ,Environmental DNA ,Endemism ,Smelt ,lcsh:Environmental sciences ,Ecology, Evolution, Behavior and Systematics - Abstract
Monitoring reproductive migration is essential for the conservation of anadromous species. Shishamo smelt (Spirinchus lanceolatus) is endemic to Hokkaido, the northernmost large island in Japan. S. lanceolatus is an anadromous species that is known to migrate into rivers for a very short period in early winter. While this species has a special value for local fisheries, the catch amount has drastically declined in the last few decades. Information about S. lanceolatus reproductive migration dynamics is limited, which prevents them from being efficiently managed as a resource. In this study, we used environmental DNA (eDNA) methods as a noninvasive molecular tool for estimating presence/absence and abundance/biomass of S. lanceolatus during their migration into rivers. We developed a species‐specific qPCR system for S. lanceolatus, examining (a) temporal variation in S. lanceolatus eDNA concentrations compared with catch data gathered by traditional methods and (b) variability of migratory patterns among river systems. In a core river for their spawning migration, we consistently detected S. lanceolatus eDNA throughout the spawning season, and the temporal distribution of eDNA concentration was consistent with that of the number of migrating S. lanceolatus estimated by catch survey data. In addition, we were able to detect S. lanceolatus eDNA even from rivers without any official record of their migration. Among rivers with eDNA detection, the relative eDNA concentrations varied, indicating that the population biomass differs largely among the river populations. Our study suggests that eDNA detection systems are useful for tracking reproductive migration of S. lanceolatus at fine spatio‐temporal scales.
- Published
- 2020
28. Bonastent for the Treatment of a Benign Central Airway Obstruction and Persistent Air Leak in a Pediatric Patient
- Author
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Karen L. Swanson, See Wei Low, Staci Beamer, Laszlo T. Vaszar, and Kenneth K. Sakata
- Subjects
Male ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Adolescent ,Iatrogenic Disease ,Self Expandable Metallic Stents ,MEDLINE ,Lymphadenopathy ,Thoracostomy ,Prosthesis Design ,Text mining ,Bronchoscopy ,Humans ,Medicine ,Central airway ,Intensive care medicine ,business.industry ,Air ,Castleman Disease ,Pneumothorax ,Airway Obstruction ,Pediatric patient ,Treatment Outcome ,Persistent air leak ,Stents ,Bronchial Fistula ,business - Published
- 2020
29. Antibacterial activity of silver/reduced graphene oxide nanocomposite synthesized by sustainable process
- Author
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Debora F. Rodrigues, Raynara Jacovone, Thaina S. Sousa, Flávia Rodrigues de Oliveira Silva, Jaqueline J.S. Soares, Hang N. Nguyen, Solange K. Sakata, and Rafael H.L. Garcia
- Subjects
Materials science ,Nanocomposite ,Ecology ,Graphene ,Oxide ,Nanoparticle ,Environmental Science (miscellaneous) ,law.invention ,Thermogravimetry ,chemistry.chemical_compound ,Silver nitrate ,chemistry ,law ,Transmission electron microscopy ,Antibacterial activity ,Energy (miscellaneous) ,Nuclear chemistry - Abstract
Traditional methods to incorporate metals into graphene oxide (GO) usually require toxic reagents or high temperatures. This study proposes an innovative and sustainable method to incorporate silver (Ag) into graphene oxide using electron beam and evaluate its antibacterial activities. The method is based on green synthesis, without toxic reagents or hazardous wastes, and can be carried out at room temperature, in short reaction times. To synthesize the Ag/rGO nanocomposite, a water/isopropanol solution with dispersed graphene oxide and silver nitrate was submitted to a dose range from 150 to 400 kGy. The product was characterized by thermogravimetry analysis, X-ray diffraction and transmission electron microscopy. The antibacterial activity of Ag/rGO was observed against Gram-negative Escherichia coli by plate count method and atomic force microscopy. The results showed that concentrations as low as 100 μg/mL of produced Ag/rGO were enough to inactivate the cells.
- Published
- 2019
30. Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA
- Author
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Izumi Katano, Toshifumi Minamoto, Teruyuki Okahashi, Hideyuki Doi, Hiroki Yamanaka, Kimiko Uchii, and Masayuki K. Sakata
- Subjects
chemistry.chemical_classification ,chemistry.chemical_compound ,Real-time polymerase chain reaction ,Chromatography ,Ecology ,chemistry ,Reagent ,Tannic acid ,Genetics ,Fulvic acid ,Humic acid ,Environmental DNA ,Ecology, Evolution, Behavior and Systematics - Published
- 2019
31. Dysphonia Due to Vocal Cord Injury After Rigid Bronchoscopy
- Author
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Stephanie J. Youssef, Terence M. Zimmermann, Diana M. Orbelo, David E. Midthun, Eric S. Edell, Darlene R. Nelson, Rebecca L. Pittelko, Dale C. Ekbom, and Kenneth K. Sakata
- Subjects
Pulmonary and Respiratory Medicine ,Rigid bronchoscopy ,medicine.medical_specialty ,Cord ,business.industry ,medicine ,business ,Surgery - Published
- 2019
32. Detection and persistence of environmental DNA (eDNA) of a vector mosquito, Culex pipiens pallens
- Author
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Masayuki K. Sakata, Megumi Sato, Tomoyuki Hikitsuchi, Jun Kobayashi, Mitsuishi Honami, Watanabe Tomoe, Toshifumi Minamoto, and Marcello Otake Sato
- Subjects
Culex pipiens pallens ,Vector (epidemiology) ,parasitic diseases ,fungi ,Zoology ,Environmental DNA ,Biology ,Persistence (computer science) - Abstract
Introduction: Preventing mosquito-borne infectious diseases requires that vector mosquitoes are monitored and controlled. Targeting immature mosquitoes (eggs, larvae, and pupae), which have less mobility than adults, is an effective management approach. However, conducting these surveys is often difficult due to the limitations of morphological classification and survey costs. The application of environmental DNA (eDNA) analysis can solve these issues because it allows easy estimation of species distribution and morphology-independent species identification. Although a few previous studies have reported mosquito eDNA detection, there is a gap in knowledge regarding the dynamics of mosquito eDNA during the developmental stages of immature mosquitoes. Methods: We used Culex pipiens pallens, a vector of West Nile fever, as a model species. First, we developed a species-specific detection assay and confirmed its specificity using in silico and in vitro tests. Next, we conducted laboratory experiments using breeding tanks. Water samples were collected at each developmental stage. In addition, water samples were collected daily until the seventh day after emergence from the pupae. We quantified eDNA using real-time PCR with the developed assay to investigate the dynamics of mosquito eDNA. Results: The specificity of the developed assay was confirmed by in silico and in vitro tests. Mosquito eDNA was detected at all developmental stages and detected up to seven days after emergence of pupae. In particular, high concentrations of eDNA were detected immediately after hatching from eggs and after emergence from pupae. Highly frequent positive eDNA signals were continuously detected between egg hatching and pupa hatching. Conclusions: Mosquito eDNA was detected immediately after the eggs were introduced, and eDNA-positive detections continued until pupae emergence, suggesting that eDNA analysis is useful for monitoring mosquito larvae. The results show that eDNA analysis provides valuable information about the water sources inhabited by immature mosquitoes in mosquito control. In the future, monitoring immature mosquitoes using eDNA analysis will aid in preventing mosquito-borne infectious diseases.
- Published
- 2021
33. Prevalence of Antimicrobial-Resistant Escherichia coli in Migratory Greater White-Fronted Geese (Anser albifrons) and their Habitat in Miyajimanuma, Japan
- Author
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Nagisa Hashimoto, Osamu Yoshida, Tetsuo Asai, Masayuki K. Sakata, Toshifumi Minamoto, Kanako Murakami, Katsumi Ushiyama, Dipti Shrestha, Akira Fukuda, Yutaka Tamura, and Masaru Usui
- Subjects
Veterinary medicine ,Nalidixic acid ,Angiotensin-Converting Enzyme Inhibitors ,Microbial Sensitivity Tests ,medicine.disease_cause ,Angiotensin Receptor Antagonists ,Antibiotic resistance ,Goose ,Anti-Infective Agents ,Japan ,biology.animal ,Drug Resistance, Bacterial ,Geese ,Escherichia coli ,Prevalence ,medicine ,Animals ,Ecosystem ,Ecology, Evolution, Behavior and Systematics ,Feces ,Ecology ,biology ,biology.organism_classification ,Antimicrobial ,Anti-Bacterial Agents ,Flock ,medicine.drug ,Anser - Abstract
The spread of antimicrobial-resistant bacteria (ARB) in natural environments including wild animals is a concern for public health. Birds cover large areas, and some fly across borders to migrate in large flocks. As a migratory bird, the Greater White-fronted Goose (Anser albifrons) travels to Miyajimanuma, North Japan, each spring and autumn. To investigate the ARB in migratory birds and their surroundings, we collected 110 fecal samples of A. albifrons and 18 water samples from Miyajimanuma in spring and autumn of 2019. Isolation of Escherichia coli was performed using selective agars with or without antimicrobials (cefazolin and nalidixic acid). Isolates of E. coli were recovered from 56 fecal samples (50.9%) and five water samples (27.8%) on agars without antimicrobials. No isolates were recovered on agars with antimicrobials. One E. coli isolate derived from a fecal sample exhibited resistance to β-lactams (ampicillin and cefazolin), whereas all other isolates exhibited susceptibility to all tested antimicrobials. The resistant isolate harbored blaACC, which could be transferred to other bacteria and confer resistance to β-lactams. These results suggest a low prevalence of antimicrobial resistance in wild migratory birds and their living environments; however, wild migratory birds sometimes carry ARB harboring transferrable antimicrobial resistance genes and therefore present a risk of spreading antimicrobial resistance.
- Published
- 2021
34. Age differences in the association between arterial velocity pulse index and muscle sympathetic nerve activity in hypertensive patients
- Author
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H Sugimoto, H Murai, T Hirai, T Hamaoka, Y Mukai, H Tokuhisa, S Usui, K Sakata, M Kawajiri, and M Takamura
- Subjects
Cardiology and Cardiovascular Medicine - Abstract
Background Increased arterial stiffness characterize by aging. It is reported that age-related increases in muscle sympathetic nerve activity (MSNA) may be one of contributing factor for arterial stiffness. Arterial reflected wave was composed of SNA and aging. Increased arterial reflected wave partly plays an important role in blood pressure. Recently, we reported that arterial velocity pulse index (AVI), a novel index of arterial reflected waves, was associated with MSNA in hypertensive patients. It is still uncertain the effect of age on the association between AVI and SNA in hypertensive patients. Method Patients with essential HT and matched non-hypertensive control subjects were included in this study. HT was diagnosed as systolic blood pressure (SBP) ≥140 mmHg or diastolic blood pressure (DBP) ≥90 mmHg. AVI was measured from left upper arm by NAS-1000. SNA was evaluated by direct recording of muscle sympathetic nerve activity (MSNA) from peroneal nerves. Results 45 HT patients and 46 control subjects were included. Age, SBP and DBP were significantly increased in HT group compared to control (Age 63±14 vs 42±16 years, p160 mmHg subjects in group 2, significant correlation was clarified between AVI and MSNA (r=0.62, p Conclusion The relationship between AVI and MSNA in HT patients is preserved regardless of aging, however, high blood pressure over 160mmHg might obscure its correlation. These results indicate that AVI is useful to estimate sympathetic nerve activity in high aging HT patient treated Funding Acknowledgement Type of funding sources: None.
- Published
- 2021
35. Different contribution of sympathetic nerve activity to arterial velocity pulse index in hypertensive patients with and without diastolic dysfunction
- Author
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H Sugimoto, H Murai, T Hirai, T Hamaoka, Y Mukai, H Tokuhisa, S Usui, K Sakata, M Kawajiri, and M Takamura
- Subjects
Cardiology and Cardiovascular Medicine - Abstract
Introduction Left ventricular diastolic dysfunction (LVDD) is the main cause of heart failure with preserved ejection fraction (HFpEF). LVDD is related not only to arterial stiffness but also sympathetic nerve activity (SNA). Recent study demonstrated that increased muscle sympathetic nerve activity (MSNA) may be one of contributing factor for arterial stiffness. In clinical practice, Cardio-ankle vascular index (CAVI) provides a reproducible index of arterial stiffness, independent of blood pressure (BP). Recently, Arterial Velocity pulse Index (AVI), which is an index of arterial reflected waves, have been proposed as new index of arterial stiffness. We reported that AVI was associated with MSNA in hypertensive (HT) patients. However, it is still uncertain the effect of LVDD on the association between AVI and SNA in HT patients. Thus, we tested the hypothesis that AVI would be increased and related to MSNA in HT patients with LVDD. Methods Patients with essential HT subjects were included in this study. HT was diagnosed as systolic blood pressure (SBP) ≥140mmHg or diastolic blood pressure (DBP) ≥90mmHg. Patients with secondary HT was excluded. AVI was measured from left upper arm by NAS-1000 (Nihon Koden, Japan). CAVI was measured by VaSera VS-1500A (Fukuda Denshi, Japan). Transthoracic echocardiography was performed by trained sonographers. SNA was evaluated by direct recording of MSNA from peroneal nerves. Results 25 HT patients were included (age 63±14 years, Male/Female 9/16). They were divided into two groups according to E/e' (no LVDD group, E/e' ≤9, N=12; LVDD group, E/e' >9, N=13). There were no significant differences between no LVDD and LVDD groups in age (63±9 vs 69±9 years p=0.205), body mass index (23±3 vs 24±4 p=0.355), BP (SBP 139±16 vs 144±20mmHg p=0.524, DBP 87±15 vs 78±14mmHg p=0.167). LV Ejection Fraction (EF) and Stroke Volume (SV) did not differ between two groups (EF 66±7 vs 69±6% p=0.471, SV 58±7 vs 62±14ml p=0.599). MSNA had tendency to increase in LVDD group compared to no LVDD group (MSNA 53±10 vs 44±12 bursts/100 heartbeats, p=0.052). Contrary to our hypothesis, AVI and CAVI did not differ between two groups (AVI 27±7 vs 29±7 p=0.398, CAVI 8.7±1.4 vs 8.6±1.4 p=0.894). However, a significant correlation was seen between AVI and MSNA in no LVDD group (r=0.57, p Conclusion AVI was significantly associated with MSNA in HT patients without LVDD, but not with LVDD. CAVI was related to AVI in HT patients with LVDD, but not without LVDD. MSNA was slightly increased in HT patients with LVDD compared to without LVDD. These results indicate that augmented SNA could contribute to the increase in arterial stiffness in HT patients without LVDD, however, this contribution might be attenuated in HT patients with LVDD. Funding Acknowledgement Type of funding sources: None.
- Published
- 2021
36. Retrospective Study of the Analgesic Effect of Sacroiliac Joint Radiofrequency Denervation
- Author
-
Ligia Ft, Kawamoto, Rioko K, Sakata, Jose L, Campos, Luis A, Borges, and Leonardo Hc, Ferraro
- Subjects
Adult ,Male ,Adolescent ,Sacroiliac Joint ,Middle Aged ,Denervation ,Young Adult ,Treatment Outcome ,Humans ,Female ,Low Back Pain ,Aged ,Pain Measurement ,Retrospective Studies - Abstract
Interventional radiofrequency (RF) ablation techniques are indicated when an adequate effect is not obtained with conservative measures.The primary objective of this study was to evaluate pain relief after RF denervation of the sacroiliac joint. The secondary objective was to evaluate pain intensity and relief duration.The study was retrospective.The study was conducted at Vera Cruz Hospital, Campinas, Brazil.Data were collected from the medical records of patients undergoing RF denervation for low back pain originating in the sacroiliac joint, from January 2015 to December 2017. There were 78 patients studied, between 18 and 65 years old, of both genders, ASA I or II, who underwent knee arthroscopic meniscectomy. The patients were submitted to denervation of sacroiliac joint by 3 types of RF (conventional, pulsed, and cooled). The following parameters were evaluated, number of patients who obtained ? 50% pain relief; pain intensity, measured using the visual analog scale (before the procedure and 15, 30, 90 and 180 days after, performed by the same evaluator); and the use of complementary analgesic for 2 weeks.Of the 78 included patients, 56 (71.8%) underwent conventional RF, 9 (11.5%) underwent pulsed RF, and 13 (16.7%) underwent cooled RF. There were losses to follow-up including 40 patients who underwent conventional RF, 5 who underwent pulsed RF, and 12 who underwent cooled RF, who were retained for 6 months. There was significant pain relief with the three types of RF for up to 6 months of follow-up, with no difference among the types. After 6 months, 90.2% of patients who underwent conventional RF, 100% who underwent pulsed RF, and 91.7% who underwent cooled RF maintained ≥ 50% pain relief. Complementary analgesics were used by 95% of the patients who underwent conventional RF, 80% who underwent pulsed RF, and 91% who underwent cooled RF 2 weeks after the procedure. There were mild adverse effects, such as edema, hematoma, and local pain, without complications.As for limitations, the number of pulsed and cooled RF is low and in a retrospective study some data may be missing, especially from follow-up.RF denervation of the sacroiliac joint is effective and promotes a long-lasting analgesic effect.
- Published
- 2021
37. Detection and persistence of environmental DNA (eDNA) of the different developmental stages of a vector mosquito, Culex pipiens pallens
- Author
-
Masayuki K. Sakata, Megumi Sato, Marcello Otake Sato, Tomoe Watanabe, Honami Mitsuishi, Tomoyuki Hikitsuchi, Jun Kobayashi, and Toshifumi Minamoto
- Subjects
Culex ,Culicidae ,Multidisciplinary ,Larva ,Pupa ,Animals ,Water ,Mosquito Vectors ,Communicable Diseases ,DNA, Environmental - Abstract
Preventing mosquito-borne infectious diseases requires that vector mosquitoes are monitored and controlled. Targeting immature mosquitoes (eggs, larvae, and pupae), which have less mobility than adults, is an effective management approach. However, conducting these surveys is often difficult due to the limitations of morphological classification and survey costs. The application of environmental DNA (eDNA) analysis can solve these issues because it allows easy estimation of species distribution and morphology-independent species identification. Although a few previous studies have reported mosquito eDNA detection, there is a gap in knowledge regarding the dynamics related to the persistence of immature mosquito eDNA. We used Culex pipiens pallens, a vector of West Nile fever, as a model species. First, we developed a species-specific detection assay and confirmed its specificity using in silico and in vitro tests. Next, we conducted laboratory experiments using breeding tanks. Water samples were collected at each developmental stage. In addition, water samples were collected daily until the seventh day after emergence from the pupae. We quantified eDNA using real-time PCR with the developed assay to investigate the dynamics of mosquito eDNA. The specificity of the developed assay was confirmed by in silico and in vitro tests. Mosquito eDNA was detected at all developmental stages and detected up to seven days after emergence of pupae. In particular, high concentrations of eDNA were detected immediately after hatching from eggs and after emergence from pupae. Highly frequent positive eDNA signals were continuously detected between egg hatching and pupa hatching. Mosquito eDNA was detected immediately after the eggs were introduced, and eDNA-positive detections continued until pupae emergence, suggesting that eDNA analysis is useful for monitoring mosquito larvae. In the future, monitoring immature mosquitoes using eDNA analysis will contribute to prevent mosquito-borne infectious diseases.
- Published
- 2022
38. Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
- Author
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Mamiko Ozaki, Masayuki K. Sakata, Satoko Nakajima, Toshifumi Minamoto, Tetsu Yasashimoto, and Tomoya Sakita
- Subjects
0106 biological sciences ,Entomology ,Science ,Biodiversity ,Hymenoptera ,010603 evolutionary biology ,01 natural sciences ,Article ,Molecular ecology ,Soil ,Argentine ant ,Animals ,Humans ,Environmental DNA ,Multidisciplinary ,biology ,Ants ,Ecology ,fungi ,food and beverages ,biology.organism_classification ,DNA, Environmental ,ANT ,010602 entomology ,Medicine ,Linepithema ,Introduced Species ,Environmental Monitoring - Abstract
Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation, or morphological identification. In this study, we demonstrated that environmental DNA (eDNA) analysis can be used as a monitoring tool for alien ants using Linepithema humile (Argentine ant), one of the most invasive ants, as a model species. We designed a new real-time PCR assay specific to L. humile and successfully detected eDNA from the surface soil. The reliability of eDNA analysis was substantiated by comparing eDNA detection results with traditional survey results. Additionally, we examined the relationship between eDNA concentration and distance from nests and trails. Our results support the effectiveness of eDNA for alien ant monitoring and suggest that this new method could improve our ability to detect invasive ant species.
- Published
- 2021
39. Robotic-Assisted Bronchoscopy to Diagnose Pulmonary Malakoplakia
- Author
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N. Azadeh, M. Smith, J. Kim, and Kenneth K. Sakata
- Subjects
medicine.medical_specialty ,Bronchoscopy ,medicine.diagnostic_test ,business.industry ,Robotic assisted ,medicine ,Malakoplakia ,Radiology ,medicine.disease ,business - Published
- 2021
40. Association of radiographic findings in hand X-ray with clinical features and autoantibodies in patients with systemic sclerosis
- Author
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Yuko Kaneko, Hidekata Yasuoka, Tsutomu Takeuchi, and K. Sakata
- Subjects
Male ,medicine.medical_specialty ,Arthritis, Rheumatoid ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Calcinosis ,Internal medicine ,Osteoarthritis ,medicine ,Humans ,030212 general & internal medicine ,skin and connective tissue diseases ,Aged ,Autoantibodies ,030203 arthritis & rheumatology ,Subluxation ,Flexion contracture ,Gastrointestinal tract ,Scleroderma, Systemic ,Acro-Osteolysis ,integumentary system ,business.industry ,Autoantibody ,Interstitial lung disease ,General Medicine ,Middle Aged ,Hand ,medicine.disease ,Dermatology ,Radiography ,Female ,business - Abstract
Musculoskeletal involvement is one of the major causes of impairment in daily life of patients with systemic sclerosis (SSc). Several hand radiographic findings can be seen in SSc patients; however, their association with clinical features and autoantibodies remains unclear. Here, we analyzed 124 SSc patients with their hand X-rays and clinical and serological features. Abnormal findings in hand X-rays including acro-osteolysis, calcinosis, flexion contracture, erosive change, joint space narrowing, and subluxation were observed in 110 patients (68%). These X-ray findings were more prevalent in patients with longer disease duration and digital ischemic changes. The majority of erosions were typical for erosive hand osteoarthritis, which was seen in 19% of patients. Hand X-ray findings were associated with involved organs; acro-osteolysis with interstitial lung disease, calcinosis with pulmonary arterial hypertension and gastrointestinal tract involvement, and flexion contracture with gastrointestinal tract involvement. Those findings were also relevant to autoantibodies; acro-osteolysis was more common in SSc patients with anti-Scl70 antibody but less in patients with anticentromere antibody. Calcinosis was more prevalent in patient with anticentromere antibody. In our study, organ involvements and SSc-associated autoantibodies showed associations with hand radiographic abnormalities. Hand X-ray findings might reflect underlying pathogenesis and autoantibody profiles in SSc patients.Key Points• Hand X-ray abnormalities were observed in approximately two-thirds of patients with SSc.• Erosive osteoarthritis was more prevalent in SSc patients than general population.• Hand X-ray findings were associated with disease duration, organ involvements, and SSc-associated autoantibodies, reflecting underlying pathogenesis.
- Published
- 2019
41. X-Ray Diffraction Evaluation of the Average Number of Layers in Thermal Reduced Graphene Powder for Supercapacitor Nanomaterial
- Author
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Luzinete Pereira Barbosa, Marilene Morelli Serna, Rubens Nunes de Faria Jr., Julio César Serafim Casini, Quezia de Aguiar Cardoso, Solange K. Sakata, Luiza F. Sobrinho, and Eguiberto Galego
- Subjects
Supercapacitor ,Materials science ,010304 chemical physics ,Graphene ,Mechanical Engineering ,Nanotechnology ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Nanomaterials ,law.invention ,Mechanics of Materials ,law ,0103 physical sciences ,Thermal ,X-ray crystallography ,General Materials Science ,0210 nano-technology - Abstract
Graphene oxide (GO) can be partially reduced to graphene-like sheets by removing the oxygen-containing groups and recovering the conjugated structure. In this work, the thermal reduction of GO powder has been carried out using back pumping vacuum pressures and investigated employing X-ray diffraction analysis. The experimental results of estimating the number of graphene layers on the reduced powder at various temperatures (200 – 1000 °C) have been reported. Electrical changes have been produced in a graphene oxide with the vacuum reduction process. This study has shown that the ideal processing temperature for reducing graphene oxide nanomaterial was about 400 °C. It has also been shown that at 600 °C the number of layers in the reduced nanomaterial increased. The internal series equivalent resistance (ESR) has been improved substantially with the vacuum thermal treatment even at temperatures above 400 °C. ESR was reduced from 95.0 to about 13.8 Ω cm2 with this processing. These results showed that the process can be applied to the reduction of graphene oxide to produce supercapacitor nanomaterials. The advantage of employing this method is that the processing is a straightforward and low cost thermal treatment that might be used for large amount of nanocomposite material.
- Published
- 2019
42. Habitat selection and migration of the common shrimp, Palaemon paucidens in Lake Biwa, Japan—An eDNA‐based study
- Author
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Ken Kawano, Masayoshi K. Hiraiwa, Hiroki Yamanaka, Ryohei Nakao, Masayuki K. Sakata, Toshiyuki Ishikawa, Toshifumi Minamoto, Satsuki Tsuji, and Qianqian Wu
- Subjects
Ecology ,Palaemon paucidens ,Biology ,environmental DNA (eDNA) ,migration ,Shrimp ,Fishery ,Quantitative Real Time PCR ,Habitat ,Lake Biwa ,Genetics ,spatial and temporal distribution ,Ecology, Evolution, Behavior and Systematics ,Selection (genetic algorithm) ,quantitative real‐time PCR - Abstract
Palaemon paucidens has a large population and is an important food source for fish in Lake Biwa, Japan. They are abundant in shallow waters from spring to summer, after which most individuals migrate to offshore deep areas where they remain during autumn and winter. However, some individuals are nonmigratory, remaining in shallow waters over winter. It has been reported that P. paucidens individuals have declined in recent years; a better understanding of its seasonal distribution is needed to manage this species, and basic information on its seasonal distribution is indispensable. We tracked the environmental DNA (eDNA) distribution of P. paucidens in Lake Biwa over a year using a quantitative real‐time polymerase chain reaction method. We collected water samples from offshore (both from the surface and from the benthic) and from shallow shore sites adjacent to the shorelines of the main lake and connecting freshwater lagoons. Offshore sampling took place in summer and winter, and shallow shore and lagoon sampling in all four seasons. During summer, eDNA concentrations were significantly higher in shallow and lagoon areas than offshore bottom sites. Conversely, during winter, eDNA concentrations were higher in offshore bottom sites, and relatively high and low eDNA concentrations in lagoons and shallow shore, respectively. These results most likely reflect the spatial and temporal distribution of this species in Lake Biwa. The eDNA concentrations peaked in early August at shallow shore sites in the main lake, with a significant decline in mid‐October, while low eDNA concentrations were recorded at offshore bottom sites in late August. These results suggest that P. paucidens migrates from shallow waters to offshore bottom sites between early August and mid‐October. These results provide important information for the management of this species.
- Published
- 2019
43. STUDY ON EXTRACTION METHODOLOGY OF RICE GROWTH CHARACTERISTICS IN FIELD USING TERRESTRIAL LASER MEASUREMENT DATA
- Author
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K. Sakata, Y. Yasunaga, and A. Rikimaru
- Subjects
lcsh:Applied optics. Photonics ,business.industry ,lcsh:T ,Point cloud ,food and beverages ,lcsh:TA1501-1820 ,Laser ,lcsh:Technology ,Field (geography) ,law.invention ,Agriculture ,law ,lcsh:TA1-2040 ,Statistics ,Range (statistics) ,Environmental science ,Spatial variability ,Extraction (military) ,Stage (hydrology) ,business ,lcsh:Engineering (General). Civil engineering (General) - Abstract
Smart agriculture is recommended for labor-saving, refinement and high quality production of rice cultivation with safety and security. The Terrestrial laser measurement has the advantage of acquiring 3D point cloud information with high accuracy and high density, but there is a disadvantage that it becomes difficult to measure the base of rice plant when paddy rice grew. In this study, the 3D point cloud information obtained by terrestrial laser measurement was used to three-dimensionally measure the growth state in the field corresponding to the growing period in time series. The growth characteristics of the whole field as a community were extracted as a representative value of mean growth. Then we attempted extraction of individual differences in growth and growth unevenness, as the deviation from the mean value of the field in terms of the growth characteristics of each strain focused on the individual plants. it is difficult to observe the root of the rice during the growing period, it was used for analyzing the growth quantity as the growth difference height for each growing period by obtaining the difference of the elevation value of the rice at the early stage of growth and at each stage of growing. The spatial variation in the growth quantity of the whole field area and the local area was calculated, the range of the deviation for evaluating the mean variation and the growth abnormality was examined, and the setting of the threshold for evaluating the growth normal and abnormality was examined.
- Published
- 2019
44. The Effect of Branched Chain Amino Acids-Enriched Nutritional Supplements on Activities of Daily Living and Muscle Mass in Inpatients with Gait Impairments: A Randomized Controlled Trial
- Author
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Kazuhisa Domen, K. Sakata, Misa Moriwaki, and Hidetaka Wakabayashi
- Subjects
Male ,Sarcopenia ,medicine.medical_specialty ,030309 nutrition & dietetics ,medicine.medical_treatment ,Medicine (miscellaneous) ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,Gait training ,law ,Internal medicine ,Acute care ,Activities of Daily Living ,medicine ,Vitamin D and neurology ,Humans ,030212 general & internal medicine ,Mobility Limitation ,Vitamin D ,Muscle, Skeletal ,Gait ,Aged ,Inpatients ,0303 health sciences ,Nutrition and Dietetics ,Rehabilitation ,business.industry ,Body Weight ,medicine.disease ,Functional Independence Measure ,Patient Discharge ,Dietary Supplements ,Female ,Geriatrics and Gerontology ,business ,Amino Acids, Branched-Chain - Abstract
To investigate the effects of continuous intervention with branched chain amino acids-enriched nutritional supplements from the acute phase to convalescent rehabilitation wards in inpatients with gait impairments. Open-label, randomized, parallel-group comparison study (UMIN Clinical Trials Registry ID: UMIN000018640). Acute care and convalescent rehabilitation wards We studied 80 patients undergoing stand/gait training. Participants in the intervention group (RJ group) received nutritional supplements (jelly foods comprising 2500 mg BCAA and 20 IU vitamin D) twice a day until hospital discharge. The primary outcome was the motor components of the Functional Independence Measure (FIM-m), and the secondary outcome was skeletal muscle mass index. Analyses were conducted on 55 patients who were able to perform stand/gait training continuously from the acute until the recovery phases. FIM-m was significantly elevated in the RJ group and the control group, but no difference was noted between the two groups. Only the RJ group showed a significant increase in skeletal muscle mass index, and the amount of variation was significantly different between the two groups (the control group decreased an average of 2.2% and the RJ group increased an average of 4.3%; P = 0.014). A significant decrease in body weight was found only in the control group (P = 0.084). Nutritional interventions using branched chain amino acids (BCAA)-enriched nutritional supplements demonstrated no significant difference in activities of daily living; however, an increase in skeletal muscle mass was noted. Skeletal muscle mass and body weight differed significantly between the two groups, and BCAA-enriched nutritional supplements intake in acute and convalescent rehabilitation wards may be effective for the prevention of malnutrition and sarcopenia.
- Published
- 2019
45. Dispersion and degradation of environmental DNA from caged fish in a marine environment
- Author
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Reiji Masuda, Satoshi Yamamoto, Tomoya Horiuchi, Akihide Kasai, Hideki Sawada, Seokjin Yoon, Michio Kondoh, Hiroaki Murakami, Yoh Yamashita, Masayuki K. Sakata, and Toshifumi Minamoto
- Subjects
Abundance (ecology) ,Dispersion (optics) ,Zoology ,Environmental science ,Degradation (geology) ,Environmental DNA ,Aquatic Science ,Transect ,Cage ,Surface water ,Bay - Abstract
Environmental DNA (eDNA) consists of DNA fragments shed from organisms into the environment, and can be used to identify species presence and abundance. This study aimed to reveal the dispersion and degradation processes of eDNA in the sea. Caged fish were set off the end of a pier in Maizuru Bay, the Sea of Japan, and their eDNA was traced at sampling stations located at the cage and 10, 30, 100, 300, 600 and 1000 m distances from the cage along two transect lines. Sea surface water was collected at each station at 0, 2, 4, 8, 24 and 48 h after setting the cage, and again after removing the cage. Quantitative PCR analyses using a species-specific primer and probe set revealed that the target DNA was detectable while the cage was present and for up to 1 h after removing the cage, but not at 2 h or later. Among the 57 amplified samples, 45 (79%) were collected within 30 m from the cage. These results suggest that eDNA can provide a snapshot of organisms present in a coastal marine environment.
- Published
- 2019
46. An unusual variant of scimitar syndrome predisposing to recurrent pneumonia
- Author
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Brandon Nokes, Niki Jackson, Kristopher W. Cummings, Laszlo T. Vaszar, and Kenneth K. Sakata
- Subjects
Pulmonary and Respiratory Medicine ,lcsh:RC705-779 ,Pediatrics ,medicine.medical_specialty ,Unusual case ,business.industry ,Partial anomalous pulmonary venous return ,Case Report ,lcsh:Diseases of the respiratory system ,respiratory system ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,030228 respiratory system ,Scimitar syndrome ,030220 oncology & carcinogenesis ,Recurrent pneumonia ,Medicine ,business - Abstract
Scimitar syndrome is a rare constellation of congenital conditions pertaining to partial anomalous pulmonary venous return. Radiographically, these anomalous pulmonary veins can resemble a scimitar, and have heterogeneous clinical presentations. We present an unusual case of scimitar syndrome with associated recurrent pneumonia. We will briefly review the literature on scimitar syndrome as well as discuss how a predisposition to recurrent pneumonia may develop in this unusual variant of a rare clinical entity.
- Published
- 2019
47. Real‐time polymerase chain reaction assays for environmental DNA detection of three salmonid fish in Hokkaido, Japan: Application to winter surveys
- Author
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Toshifumi Minamoto, Kana Hayami, Akio Imamura, and Masayuki K. Sakata
- Subjects
Rainbow Trout (Oncorhynchus mykiss) ,0106 biological sciences ,010604 marine biology & hydrobiology ,Zoology ,environmental DNA (eDNA) ,Biology ,Salmonid fish ,winter ,010603 evolutionary biology ,01 natural sciences ,Real-time polymerase chain reaction ,Dolly Varden (Salvelinus malma) ,Environmental DNA ,Whitespotted Char (S. leucomaenis) ,Ecology, Evolution, Behavior and Systematics - Abstract
Dolly Varden (Salvelinus malma) and Whitespotted Char (Salvelinus leucomaenis) are representative native fish of the family Salmonidae that inhabit the upper reaches of rivers on Hokkaido Island, Japan. They are threatened by the invasive Rainbow Trout (Oncorhynchus mykiss). In this study, environmental DNA (eDNA) real‐time polymerase chain reaction (PCR) assays to detect these three salmonids were developed and used to clarify the distribution pattern of these fish. A specificity test for each assay was conducted using DNA extracted from both target and closely related fish, and the specificity of each assay was confirmed. Then, we carried out eDNA surveys in two mountainous rivers around Mt. Daisetsu in winter, when snow depth was maximized. In the winter surveys, eDNA of all three species were successfully detected from river water samples, including under‐ice water samples. The results of eDNA detection corresponded with the results of an earlier distribution survey performed with Japanese‐style fly‐fishing and lure‐fishing. These results suggested that the eDNA assays developed in this study are applicable for inter‐seasonal surveys for these species.
- Published
- 2018
48. Refractory IgG4-related Pleural Disease with Chylothorax: A Case Report and Literature Review
- Author
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Yuichiro Ota, Naoshi Nishina, Katsura Emoto, Jun Kikuchi, Tsutomu Takeuchi, Yuko Kaneko, Tomomi Kotaki, Hironari Hanaoka, Katsuya Suzuki, Kotaro Otomo, and K. Sakata
- Subjects
Male ,medicine.medical_specialty ,Pleural effusion ,Case Report ,030204 cardiovascular system & hematology ,Chylothorax ,Thoracic duct ,03 medical and health sciences ,Pleural disease ,0302 clinical medicine ,rituximab ,Refractory ,Internal Medicine ,medicine ,Humans ,IgG4-related disease ,Pleurisy ,Aged ,business.industry ,pleuritis ,General Medicine ,medicine.disease ,Surgery ,Pleural Effusion ,medicine.anatomical_structure ,Immunoglobulin G ,Prednisolone ,Pleura ,030211 gastroenterology & hepatology ,Rituximab ,Immunoglobulin G4-Related Disease ,business ,medicine.drug - Abstract
We herein report a rare case of a 66-year-old man with refractory chylothorax. Although he had been treated with moderate doses of prednisolone (PSL) on suspicion of pleuritis with Sjogren syndrome, the pleural effusion expanded after the reduction of prednisolone. Further workup including histopathological examinations of pleura led to the diagnosis of IgG4-RD with bilateral chylothorax without any leakage from the thoracic duct. Combination therapy with high-dose PSL plus rituximab successfully decreased the pleural effusion. This is a very rare case of IgG4-related pleuritis with chylothorax and the first report of its successful treatment with rituximab.
- Published
- 2021
49. Safety and tolerability of ultrasound-guided synovial needle biopsy in Japanese arthritis patients
- Author
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Tsutomu Takeuchi, Chihiro Takahashi, Katsuya Suzuki, Y. Inoue, Yuko Kaneko, Yasushi Kondo, and K. Sakata
- Subjects
musculoskeletal diseases ,Image-Guided Biopsy ,medicine.medical_specialty ,Arthritis ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Japan ,Biopsy ,medicine ,Humans ,030212 general & internal medicine ,Ultrasonography, Interventional ,030203 arthritis & rheumatology ,medicine.diagnostic_test ,business.industry ,Ultrasound ,Biopsy, Needle ,medicine.disease ,Ultrasound guided ,Ultrasound guidance ,Tolerability ,Needle biopsy ,Radiology ,business - Abstract
We sought to clarify the safety and tolerability of ultrasound (US)-guided synovial needle biopsy in hand and knee joints in Japanese arthritis patients.A total of nine consecutive arthritis patients were recruited and scheduled for US-guided synovial needle biopsies. Patients completed a safety questionnaire and patient-reported outcomes (PRO) data of joint pain, stiffness, and swelling pre- and postbiopsy. We also recorded patients' characteristics and willingness to undergo a second biopsy by the same technique. All synovial needle biopsy samples were assessed with pathological and microbial examination to verify whether clinical evaluation was possible.Five and 4 patients underwent US-guided biopsy from hand and knee joints, respectively. PRO data showed no significant differences in pain, swelling, or stiffness levels before and after biopsy, with a mean 11.8 samples collected per procedure. No significant complications, including joint infection, bleeding, or vasovagal signs, were reported. Histologically adequate synovial tissue was identified in 83 (78%) samples. We were able to submit the biopsy samples to pathological and bacterial analysis to exclude septic arthritis.We demonstrated that a minimally invasive US-guided needle biopsy is a safe and well-tolerated procedure and the synovial tissue collected was of adequate quality for pathological analysis.
- Published
- 2020
50. The outcome of ablation for non-paroxysmal atrial fibrillation targeting spatiotemporal electrogram dispersion compared with ganglionated plexi ablation
- Author
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S Yamashita, K Yamashiro, T Tanaka, and K Sakata
- Subjects
medicine.medical_specialty ,Ejection fraction ,business.industry ,Paroxysmal atrial fibrillation ,medicine.medical_treatment ,Atrial fibrillation ,Cardiac Ablation ,medicine.disease ,Ablation ,medicine.anatomical_structure ,Internal medicine ,Direct current cardioversion ,medicine ,Cardiology ,Right atrium ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Atrial tachycardia - Abstract
Background Although catheter ablation targeting ganglionated plexi (GP) playing an important role in formation of triggers and substrates of atrial fibrillation (AF) has been reported as one of the effective ablation strategies in non-paroxysmal AF (non-PAF) patients, its effectiveness varies among the study groups. More recently, ablation targeting spatiotemporal electrogram dispersion (STED) areas, assumed to contain AF drivers in forms of rotational activation is proposed. However, the optimal ablation strategy for non-PAF is still controversial since the exact mechanisms of non-PAF are not well understood. Purpose To investigate the effectiveness of GP ablation for autonomic modification and STED ablation for modulation of AF drivers. Methods Consecutive 149 non-PAF patients who underwent STED ablation in our center were enrolled. We detected STED areas within the whole left and right atrium during AF using PentaRay®, and ablated them. If AF was terminated during STED ablation, we finished the procedure without burning the remaining STED areas. If not, electrical cardioversion was applied. The outcome was compared with that in consecutive 156 non-PAF patients undergoing GP ablation previously in our center. Results (1) The clinical characteristics were comparable between two groups (see Table). (2) A Kaplan-Meier curve showed that there was no significant difference between the freedom rates from non-PAF/non-paroxysmal atrial tachycardia (non-PAT) after single procedure in STED group and GP group (Figure, left). (3) However, the freedom rates from non-PAT in STED group was significantly lower than that GP group (Figure, right). Conclusions The recurrence type of atrial arrhythmia after ablation was remarkably different between ablation of STED and GP. STED ablation might eliminate fibrillatory conduction and control AF driver in patients with non-PAF. Freedom from atrial arrhythmia Funding Acknowledgement Type of funding source: None
- Published
- 2020
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