178 results on '"José Carlos Tavares Carvalho"'
Search Results
2. Pharmacological Potential and Mechanisms of Action Involved in Oil Species from the Brazilian Amazon: The Case of Abelmoschus esculentus L. Moench, Euterpe oleracea Martius and Bixa orellana Linné
- Author
-
Priscila Faimann Sales, Rosemary de Carvalho Rocha Koga, Agerdanio Andrade de Souza, Aline Lopes do Nascimento, Fernanda Cavalcante Pinheiro, Andressa Ketelem Meireles Alberto, Madson Jonhe da Costa, and José Carlos Tavares Carvalho
- Subjects
Pharmacology ,Complementary and alternative medicine ,Drug Discovery ,Plant Science - Published
- 2023
3. Ormona® SI and Ormona® RC—New Nutraceuticals with Geranylgeraniol, Tocotrienols, Anthocyanins, and Isoflavones—Decrease High-Fat Diet-Induced Dyslipidemia in Wistar Rats
- Author
-
Ana Paula Santos Rodrigues, Raimundo da Silva Barbosa, Arlindo César Matias Pereira, Mateus Alves Batista, Priscila Faimann Sales, Adriana Maciel Ferreira, Nayara Nilcia Dias Colares, Heitor Ribeiro da Silva, Marcelo Oliveira dos Santos Soares, Lorane Izabel da Silva Hage-Melim, and José Carlos Tavares Carvalho
- Abstract
Dyslipidemia is a metabolic condition linked to increased morbidity. This study aimed to evaluate the effect of two new nutraceuticals derived from annatto (Bixa orellana), açaí (Euterpe oleracea), and soybean germ or red clover isoflavones (Trifolium pratense) against high-fat-induced dyslipidemia in female Wistar rats. The formulations were assessed through GC-MS and HPLC-UV/Vis. Next, female Wistar rats received daily administrations of coconut oil and were treated with Ormona® SI (OSI), Ormona® RC (ORC), soybean germ (SG), simvastatin (simv.), simvastatin + Ormona® SI (simv. + OSI), or only distilled water (control). Biochemical tests were performed using the animals’ blood, and their arteries were screened for atheroma formation. The results show abnormal TC, TG, LDL, and HDL levels caused by the high-fat diet, increased glucose levels, hepatotoxicity, nephrotoxicity, and atherogenesis. The nutraceuticals significantly ameliorated all conditions, with results that are similar to the group treated with simvastatin. Notably, the groups treated with either Ormona® or simvastatin + Ormona® had better liver protection than those treated only with SG or simvastatin; additionally, the nutraceuticals could prevent atherogenesis, unlike SG. The results indicate a high efficacy of the nutraceuticals in preventing dyslipidemia and its complications.
- Published
- 2022
4. Morphological and Phylogenetic Features of Ceratomyxa macapaensis n. sp. (Myxozoa: Ceratomyxidae) in Mesonauta festivus Heckel, 1840 (Cichliformes: Cichlidae) from the Eastern Amazon Region
- Author
-
José Carlos Tavares Carvalho, Abthyllane Amaral de Carvalho, Marcela Videira, Edilson Matos, Marcelo Francisco da Silva, Luana Silva Bittencourt, Igor Hamoy, and Diehgo Tuloza da Silva
- Subjects
Myxozoa ,Phylogenetic tree ,biology ,Parasitic Diseases, Animal ,Spores, Protozoan ,Gallbladder ,Zoology ,Cichlids ,biology.organism_classification ,Mesonauta festivus ,Spore ,Fish Diseases ,Taxon ,Genus ,Animals ,Humans ,Parasite hosting ,Parasitology ,Ceratomyxa ,Brazil ,Phylogeny - Abstract
The class Myxozoa consists of microscopic spores that typically present uniform morphological simplifications, containing one or two sporoplasms surrounded by valve cells. The present study analysed the morphological and phylogenetic characteristics of what proved to be a new species of Ceratomyxa found in the gallbladder of Mesonauta festivus Heckel, 1840 captured on the Piririm River in the municipality of Macapa, in Amapa state, Brazil. The fish were collected in gillnets, and were transported alive to Amapa State University in Macapa for the analysis of the organs. Crescent- or arch-shaped free spores were observed under light microscopy, containing two polar capsules and a suture line, which is typical of the morphology of the genus Ceratomyxa, contained within plasmodia that had snake-like movements. The gallbladder was conserved in 80% alcohol for the analysis of the 18S rDNA gene, and in Davidson solution for standardhistology. Ceratomyxa spores (n = 20) were observed in 11 of the 50 samplesanalysed, and thus had a prevalence of 22%, and had a mean length of 4.2 ± 0.5 µm, mean thickness of 22.75 ± 0.3 µm, and two polar capsules, 1.86 ± 0.3 µm long and 1.63 ± 0.1 µm thick. The phylogenetic analysis indicated that the new species forms a cluster with other freshwater Amazonian Ceratomyxa species, described previously. The new species represents the first description of a taxon of the genus Ceratomyxain fish from the Brazilian state of Amapa.
- Published
- 2021
5. Characterization of the essential oil from Annona acutiflora and its nanoemulsion for the Aedes aegypti control
- Author
-
Raimundo Nonato Picanço Souto, Jonatas Lobato Duarte, Francisco P. Machado, José Carlos Tavares Carvalho, Leandro Rocha, Ricardo dos Santos Esteves, Ricardo Marcelo dos Anjos Ferreira, Anna E.M.F.M. Oliveira, Marcelo Guerra Santos, Bettina Monika Ruppelt, Rodrigo A.S. Cruz, Caio P. Fernandes, and Diogo Folly
- Subjects
Traditional medicine ,law ,General Chemistry ,Aedes aegypti ,Biology ,biology.organism_classification ,Annona ,Larvicide ,Essential oil ,law.invention - Abstract
This work describes the chemical composition of the essential oil from Annona acutiflora and its larvicidal nanoemulsion. The volatile oil was extracted by hydrodistillation. The chemical character...
- Published
- 2021
6. Acute and Reproductive Toxicity Evaluation of Ormona
- Author
-
Clarice Flexa, da Rocha, Camila de Nazaré Nunes, Flexa, Gisele Custodio, de Souza, Arlindo César Matias, Pereira, Helison de Oliveira, Carvalho, Aline Lopes, do Nascimento, Natasha Juliana Perdigão, de Jesus Vasconcelos, Heitor Ribeiro, da Silva, and José Carlos Tavares, Carvalho
- Abstract
The zebrafish is a popular organism to test the toxicity of compounds. Here, we evaluate the acute and reproductive toxicity of Ormona SI
- Published
- 2022
7. Acute Toxicity and Anti-Inflammatory Activity of
- Author
-
Agerdânio Andrade, de Souza, Brenda Lorena Sánchez, Ortíz, Swanny Ferreira, Borges, Andria Vanessa Pena, Pinto, Ryan da Silva, Ramos, Igor Colares, Pena, Rosemary de Carvalho, Rocha Koga, Carla Estefani, Batista, Gisele Custódio, de Souza, Adriana Maciel, Ferreira, Sergio, Duvoisin Junior, and José Carlos, Tavares Carvalho
- Subjects
Molecular Docking Simulation ,Plant Extracts ,Phytochemicals ,Anti-Inflammatory Agents ,Animals ,Edema ,Esters ,Carrageenan ,Burseraceae ,Zebrafish - Abstract
The species
- Published
- 2022
8. Bauhinia guianensis Aubl., a Plant from Amazon Biome with Promising Biologically Active Properties: A Systematic Review
- Author
-
Rosângela do Socorro Ferreira Rodrigues Sarquis, José Carlos Tavares Carvalho, Abrahão Victor Tavares de Lima Teixeira dos Santos, and Rosemary de Carvalho Rocha Koga
- Subjects
Pharmacology ,Complementary and alternative medicine ,Amazon rainforest ,Drug Discovery ,Botany ,Biome ,Bauhinia guianensis ,Plant Science ,Biology - Published
- 2021
9. In vitro anthelminthic activity of Lippia alba essential oil combined with silk fibroin against monogeneans of Colossoma macropomum (Serrasalmidae)
- Author
-
José Carlos Tavares Carvalho, Joziele Neves Nogueira, Fernando B. Neves, Irlon M. Ferreira, Marcos Tavares-Dias, Carliane Maria Guimarães Alves, Ligia Rigôr Neves, and Andria Vanessa Pena Pinto
- Subjects
biology ,Traditional medicine ,law ,Fibroin ,Aquatic Science ,biology.organism_classification ,Phytotherapy ,Serrasalmidae ,Lippia alba ,In vitro ,Essential oil ,law.invention - Published
- 2021
10. Valeiridoside, an Iridoid Xyloside from Valeriana procera with Anxiogenic Effect in Mice
- Author
-
José Luis Balderas-López, José Carlos Tavares-Carvalho, Alejandro Alfaro-Romero, and Andrés Navarrete
- Subjects
Ketanserin ,biology ,Iridoid ,010405 organic chemistry ,medicine.drug_class ,Chemistry ,GABAA receptor ,Pharmacology ,biology.organism_classification ,01 natural sciences ,Anxiolytic ,0104 chemical sciences ,Xyloside ,010404 medicinal & biomolecular chemistry ,Anxiogenic ,medicine ,General Pharmacology, Toxicology and Pharmaceutics ,Caprifoliaceae ,5-HT receptor ,medicine.drug - Abstract
Valeriana procera Kunth, Caprifoliaceae, is a medicinal plant used in Mexican traditional medicine as a mild sedative, tranquilizer, and sleep-aid agent. This species possesses several pharmacological effects including sedative, anxiolytic, and vasorelaxant. The chemical investigation of this plant has led to the isolation of an iridoid xyloside from the ethanol extract prepared with roots which were named valeiridoside, a β-d-xyloside of the iridolactone patriscabrol. This compound, paradoxically, showed an anxiogenic effect in mice at 10 mg/kg (i.p.), which was inhibited by ketanserin (2.5 mg/kg, i.p.) and WAY 100635 (5 mg/kg, i.p.), but not for diazepam (2.5 mg/kg, i.p.) or NECA (0.00547 mg/kg, i.p.). These results suggest the participation of 5-HT2 and 5-HT1A serotonin receptors, but not the involvement of GABAA or adenosine receptors in the anxiogenic effect of valeiridoside.
- Published
- 2021
11. Trichoderma asperellum Extract Isolated from Brazil Nuts (Bertholletia excelsa BONPL): In Vivo and In Silico Studies on Melanogenesis in Zebrafish
- Author
-
Adriana Maciel Ferreira, Iracirema da Silva Sena, Jhone Curti, Agerdânio Andrade de Souza, Paulo Cesar dos Santos Lima, Alex Bruno Lobato Rodrigues, Ryan da Silva Ramos, Wandson Braamcamp de Souza Pinheiro, Irlon Maciel Ferreira, and José Carlos Tavares Carvalho
- Subjects
Amazon fungi ,Danio rerio ,fungus extract ,pigmentation ,Microbiology (medical) ,Virology ,Microbiology ,pharmacology_toxicology - Abstract
Endophytic fungi are those that present part of their life cycle in healthy tissues of different plant hosts in symbiosis without causing harm. At the same time, fungus-plant symbiosis makes it possible for microorganisms to synthesize their own bioactive secondary metabolites while in stationary stage. Such microorganism-derived secondary metabolites contribute. Therefore, this work aimed to. To accomplish this, the endophytic fungus Trichoderma asperellum was isolated from Bertholletia excelsa (Brazil nut) almonds. The fungus was cultivated and extracted with ethyl acetate, obtaining AM07Ac. Then, using HPTLC (High-performance thin-layer chromatography) and Nuclear Magnetic Resonance (1H NMR), β-amyrin, kaempferol, and brucine were identified as major compounds. Further in vivo assays in Zebrafish demonstrated the activity of AM07Ac on melanogenesis by producing a concentration-response inhibitory effect, which, through an in silico study, proved to be related to the noted major compounds known to inhibit tyrosinase activity. The inhibition of tyrosinase prevents melanin accumulation in skin. Therefore, these results imply the importance of investigating microorganisms and their pharmacological activities, in particular the endophytic fungus Trichoderma asperellum as a generator of active metabolites for melanogenesis modulation.
- Published
- 2023
12. Clinical Safety of a Pharmaceutical Formulation Containing an Extract of Acmella oleracea (L.) in Patients With Premature Ejaculation: A Pilot Study
- Author
-
Lecildo Lira Batista, Rosemary de Carvalho Rocha Koga, Abrahão Victor Tavares de Lima Teixeira, Thiago Afonso Teixeira, Ester Lopes de Melo, and José Carlos Tavares Carvalho
- Subjects
Health (social science) ,Public Health, Environmental and Occupational Health - Abstract
Acmella oleracea (L.) R. K. Jansen (Asteraceae) is a plant species widely used in traditional Amazonian medicine to treat sexual dysfunction. The use of this plant has gained popularity because of its sensory properties, such as a tingling sensation. In this study on patients with premature ejaculation, we evaluated the clinical action of a nano-formulation containing an ethanolic extract of A. oleracea inflorescences. Major constituents in the extracts were identified based on gas chromatographic analysis. Participants used a spray preparation based on the A. oleracea extract for 12 weeks, during which they were instructed to apply the product 5 min prior to sexual intercourse. To assess therapeutic efficacy, participants were required to record the mean intravaginal latency time for ejaculation (IELT). During the period of spray treatment, the nano-formulation of A. oleracea increased participant IELT values ( M = 293 s) compared with the baseline values (193 s). This nano-formulation reported clinical action in patients with premature ejaculation. It is accordingly considered to have potential application as a therapeutic alternative with benefits for both patients and their partners. Given the small number of participants in this study, further multicenter studies involving a larger number of participants are needed to confirm these observations.
- Published
- 2023
13. Perillyl alcohol decreases the frequency and severity of convulsive-like behavior in the adult zebrafish model of acute seizures
- Author
-
Arlindo César Matias Pereira, Lorane Izabel da Silva Hage-Melim, Xuebo Hu, José Carlos Tavares Carvalho, Brenda Lorena Sánchez-Ortiz, Ester Lopes de Melo, and Raphaelle Sousa Borges
- Subjects
Male ,0301 basic medicine ,medicine.medical_treatment ,Positive control ,Negative control ,Pharmacology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Seizures ,In vivo ,medicine ,Animals ,Zebrafish ,Diazepam ,Behavior, Animal ,Dose-Response Relationship, Drug ,biology ,business.industry ,Perillyl alcohol ,Patient Acuity ,General Medicine ,biology.organism_classification ,Molecular Docking Simulation ,Disease Models, Animal ,030104 developmental biology ,Anticonvulsant ,Mechanism of action ,chemistry ,Monoterpenes ,Pentylenetetrazole ,Anticonvulsants ,Female ,medicine.symptom ,business ,030217 neurology & neurosurgery ,medicine.drug - Abstract
This research aimed to assess the effect of perillyl alcohol (PA) on convulsive behavior in vivo using adult zebrafish (Danio rerio, both sexes). The seizures were induced with pentylenetetrazole (PTZ) intraperitoneally at 170 mg/kg, and diazepam (DZP) was used as the control anticonvulsant (2 mg/kg, oral); PA was tested at 10, 50, and 100 mg/kg orally. The groups had ten animals per group (total n = 60), observed for 10 minutes after seizure induction. We manually appraised typical seizure phenotypes for quantification and used an animal tracking software (Toxtrac) to assess the motor parameters. Next, we sought to find a mechanism of action for PA anticonvulsant activity in silico using a structure-based activity prediction server and molecular docking. The results show that PTZ induced seizure-like behavior in all untreated animals with hyperlocomotion episodes, seizure itself, posture loss, and immobility. DZP inhibited the seizures in all animals of the positive control group. PA, in turn, inhibited the occurrence of seizures in a dose-dependent manner, with frequencies of 90%, 70%, and 40% (for 10, 50, and 100 mg/kg, respectively). The PA treatments also decreased several seizure endpoints in a dose-dependent manner. Also, the difference of the group treated with highest dose of PA was statistically significant compared with the negative control group for all the endpoints assessed (p < 0.05, Kruskal-Wallis). The in silico analyses suggested that PA can affect the GABAergic system, which might be involved in its anticonvulsant activity, but other mechanisms cannot be ruled out. Overall, our results suggest an anticonvulsant potential in perillyl alcohol.
- Published
- 2021
14. The effects of Rosmarinus officinalis L. essential oil and its nanoemulsion on dyslipidemic Wistar rats
- Author
-
Mehl Letícia Elizandra Boettger, Belmira Silva Faria e Souza, Jonatas Lobato Duarte, Arlindo César Matias Pereira, Robson Barbosa, Albenise Santana Alves Barros, Ana Paula Santos Rodrigues, Adriana Maciel Ferreira, Helison de Oliveira Carvalho, Caio P. Fernandes, José Carlos Tavares Carvalho, and Irlon M. Ferreira
- Subjects
Simvastatin ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,Hypercholesterolemia ,Biomedical Engineering ,General Biochemistry, Genetics and Molecular Biology ,Rosmarinus ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Artificial Intelligence ,Internal medicine ,Oils, Volatile ,medicine ,Animals ,Rats, Wistar ,General Pharmacology, Toxicology and Pharmaceutics ,Triglycerides ,Dyslipidemias ,Hypertriglyceridemia ,General Immunology and Microbiology ,biology ,Cholesterol ,business.industry ,General Neuroscience ,nutritional and metabolic diseases ,Lipid metabolism ,Cholesterol, LDL ,General Medicine ,Atherosclerosis ,medicine.disease ,biology.organism_classification ,Rats ,030205 complementary & alternative medicine ,Endocrinology ,chemistry ,030220 oncology & carcinogenesis ,Officinalis ,lipids (amino acids, peptides, and proteins) ,Nanoparticle Drug Delivery System ,General Agricultural and Biological Sciences ,business ,Dyslipidemia ,medicine.drug ,Lipoprotein - Abstract
Dyslipidemias are lipid metabolism alterations that cause increased levels of serum lipoprotein, cholesterol, and triglycerides. These alterations are associated with a higher incidence of cardiovascular diseases and are a risk factor for atherosclerosis development. This study aimed to evaluate the effect of Rosmarinus officinalis essential oil (EORO, 100 mg/kg) and its nanoemulsion (NEORO, 500 µg/kg) on Triton and coconut saturated-fat-induced (CSF) dyslipidemias using Wistar rats. The phytochemical evaluation of EORO performed by gas chromatography-mass spectroscopy (GC-MS) revealed 1,8-cineole (33.70%), camphor (27.68%), limonene (21.99%), and α-pinene (8.13%) as its major compounds. Triton-induced dyslipidemia significantly increased total cholesterol, LDL, and triglycerides levels. On the other hand, the groups treated with EORO and NEORO had significantly reduced total cholesterol, LDL, and triglycerides compared to the group treated only with Triton. Similar results were observed on the positive control treated with simvastatin. Dyslipidemia induced with coconut saturated-fat (CSF) caused abdominal fat gain, hypercholesterolemia, hypertriglyceridemia, increased LDL levels, and atherogenesis in the aorta. In contrast, the groups treated with EORO, NEORO, and simvastatin had significantly reduced hypercholesterolemia and hypertriglyceridemia, reduced abdominal fat gain, and absence of atherogenesis in the vascular endothelium. Overall, in the Triton-induced dyslipidemia model, EORO treatment had superior values than NEORO's (and simvastatin), although the differences were not too high, while in the CSF model, the values were mixed. In this manner, our results show an anti-dyslipidemic and anti-atherogenic activity effect by EORO and NEORO.
- Published
- 2020
15. Actions of Cannabis sativa L. fixed oil and nano-emulsion on venom-induced inflammation of Bothrops moojeni snake in rats
- Author
-
José Carlos Tavares Carvalho, Karyny Roberta Tavares Picanço, Maria Lucia, Abrahão Victor Tavares de Lima Teixeira dos Santos, Danna Emanuelle Santos Gonçalves, Irlon M. Ferreira, Xuebo Hu, Helison de Oliveira Carvalho, and Caio P. Fernandes
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Immunology ,Anti-Inflammatory Agents ,Peritonitis ,Inflammation ,Pharmacology ,Bothrops moojeni ,03 medical and health sciences ,Gastrocnemius muscle ,0302 clinical medicine ,Edema ,Crotalid Venoms ,mental disorders ,medicine ,Animals ,Plant Oils ,Bothrops ,Pharmacology (medical) ,Centrifugation ,Particle Size ,Rats, Wistar ,Muscle, Skeletal ,Cannabis ,biology ,Chemistry ,biology.organism_classification ,medicine.disease ,Nanostructures ,Rats ,030104 developmental biology ,Snake venom ,Emulsions ,Histopathology ,medicine.symptom ,030217 neurology & neurosurgery - Abstract
Bothrops moojeni snake venom (VBm) has toxins that cause pronounced tissue damage and exacerbated inflammatory reaction. Cannabis sativa L. is a plant species that produces an oil (CSO) rich in unsaturated fatty acids. Nano-emulsions have several advantages, such as better stability and higher penetrating power in membranes. Therefore, this study evaluated the effect of a nano-emulsion based on this herbal derivative (NCS) against VBm-induced inflammation in Wistar rats. The CSO and NCS were submitted to physicochemical characterization. The inflammatory process was induced by the VBm (0.10 mg/kg) as follows: rat paw edema, peritonitis, analysis of leukocyte infiltrate in gastrocnemius muscle of rats and formation of granulomatous tissue. No significant changes were observed when the NCS was submitted to the centrifugation and thermal stress tests. There was no phase separation, changes in density (0.978 ± 0.01 g/cm3) and viscosity (0.889 ± 0.15). The droplet diameter ranged from 119.7 ± 065 to 129.3 ± 0.15 nm and the polydispersity index ranged from 0.22 ± 0.008 to 0.23 ± 0.011. The results showed that treatments with CSO (200 and 400 mg/kg) and NCS (100 mg/kg) were able to decrease significantly (p
- Published
- 2020
16. Nano-emulsification of Aeollanthus suaveolens Mart. Ex Spreng essential oil modifies its neuroeffects?
- Author
-
Fabio Rocha Formiga, Diego Quaresma Ferreira, Caio P. Fernandes, José Carlos Tavares Carvalho, Rosa Helena Veras Mourão, and Thamara de Oliveira Ferraz
- Subjects
Dispersity ,Pharmaceutical Science ,02 engineering and technology ,030226 pharmacology & pharmacy ,Physical property ,law.invention ,Surface-Active Agents ,03 medical and health sciences ,0302 clinical medicine ,Pulmonary surfactant ,Dynamic light scattering ,law ,Oils, Volatile ,Animals ,Nanotechnology ,Plant Oils ,Zebrafish ,Essential oil ,Lamiaceae ,biology ,Chemistry ,technology, industry, and agriculture ,Aqueous two-phase system ,Water ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Chemical engineering ,Drug delivery ,Aeollanthus ,Emulsions ,0210 nano-technology - Abstract
Oil in water nano-emulsions are drug delivery systems constituted by liquid lipophilic nano-droplets dispersed through the external aqueous phase, often reaching the kinetic stability with surfactant as stabilizers. Essential oils can be the oily phase or the source of bioactive compounds. In this study, the essential oil of Aeollanthus suaveolens-a plant used in folk medicine due to its psychopharmacological effects-was used for preparation of fine nano-emulsions by a low-energy titrating method. Monodisperse small nano-droplets (ca. 70 nm; PdI 0.200) were assembled by using blends of non-ionic surfactants, indicating modulation on surfactant system lead to altering the physical property. In a separate set of experiments, we investigated the role of this modulation on biological properties of the optimal nano-emulsion. The zebrafish embryos were more susceptible to the nano-emulsion than the bulk essential oil, showing the improved bioactivity due to nano-sizing. Therefore, adult zebrafish was treated, and paralysis was observed in the groups treated with the nano-emulsion, being this finding in accordance with hypnosis. At the same essential oil dose, another behavior was observed, suggesting that expected dose-dependent effects associated to sedative-hypnotics can be achieved by nano-sizing of psychoactive essential oils. This paper contributes to the state-of-art drug delivery systems by opening perspectives for novel sedative-hypnotics nano-emulsified essentials oils that can reach hypnotic effects at considerably lower dose, when compared with bulk materials, being useful for further completed dose-response studies.Graphical abstract.
- Published
- 2020
17. Anti-inflammatory, antioxidant, and gaso-protective mechanism of 3α-hydroxymasticadienoic acid and diligustilide combination on indomethacin gastric damage
- Author
-
José Luis Balderas-López, Aracely Evangelina Chávez-Piña, Alejandra Orona-Ortiz, Josué A. Velázquez-Moyado, Andrés Navarrete, José Carlos Tavares-Carvalho, and Elizabeth Arlen Pineda-Peña
- Subjects
Male ,0301 basic medicine ,Antioxidant ,medicine.drug_class ,medicine.medical_treatment ,Indomethacin ,Pharmacology toxicology ,Anti-Inflammatory Agents ,Pharmacology ,Nitric Oxide ,Leukotriene B4 ,Antioxidants ,Dinoprostone ,Anti-inflammatory ,03 medical and health sciences ,Basal (phylogenetics) ,0302 clinical medicine ,medicine ,Animals ,Hydrogen Sulfide ,Stomach Ulcer ,Rats, Wistar ,chemistry.chemical_classification ,Superoxide Dismutase ,Tumor Necrosis Factor-alpha ,Chemistry ,Mucosal lesions ,General Medicine ,Anti-Ulcer Agents ,Triterpenes ,Disease Models, Animal ,030104 developmental biology ,Enzyme ,Gastric Mucosa ,030220 oncology & carcinogenesis ,Drug Therapy, Combination ,Gastric injury ,Fixed ratio - Abstract
The co-administration of 3α-hydroxymasticadienoic acid (3α-OH MDA) and diligustilide (DLG) generates a synergist gastroprotective effect on indomethacin-induced gastric damage. However, the related protective activities of the compounds alone (or in combination) remain unclear. In the present study, we evaluated the anti-inflammatory and antioxidative activities, as well as the potential modulation of important gasotransmitters of each compound individually and in combination using the indomethacin-induced gastric damage model. Male Wistar rats were treated orally with the 3α-OH MDA, DLG, or their combination (at a fixed ratio of 1:1, 1:3, and 3:1) 30 min before the generation of gastric mucosal lesions with indomethacin (30 mg/kg, p.o.). Three hours later, the gastric injury (mm2) was determined. Results from these experiments indicate, in addition to maintaining basal levels of PGE2, the gastroprotective effect of the pre-treatment with 3α-OH MDA (70%), DLG (81%), and their combination (72%) which was accompanied by significant decreases in leukocyte recruitment, as well as decreases in TNF-α and LTB4 gastric levels (p
- Published
- 2020
18. Gastroprotective Properties of Nanoemulsion of Ligusticum porteri Volatile Oil in Rats
- Author
-
Itzel Karina Vega-Aguilar, Andrés Navarrete, Aracely Evangelina Chávez-Piña, Josué A. Velázquez-Moyado, José Carlos Tavares-Carvalho, and Elizabeth Arlen Pineda-Peña
- Subjects
Apiaceae ,biology ,Traditional medicine ,010405 organic chemistry ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Nitric oxide ,Phthalide ,law.invention ,Steam distillation ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,law ,medicine ,Ligusticum porteri ,General Pharmacology, Toxicology and Pharmaceutics ,Omeprazole ,medicine.drug - Abstract
The roots of Ligusticum porteri J.M. Coult. & Rose, Apiaceae, are used in Mexican traditional medicine for the treatment of gastrointestinal disorders. The dimeric phthalide (Z´)-diligustilide found in their organic extracts is associated with the gastroprotective properties. In this study, the gastroprotective effect of nanoemulsion of the volatile oil from the roots of L. porteri (at doses of 1, 3, 10, and 30 mg/kg) in the indomethacin-induced gastric damage model was tested, and nitric oxide (NO) and hydrogen sulfide (H2S) gastric levels were determinate. The volatile oil obtained by steam distillation (0.63% yield) contained 37 constituents, as assessed by GC-MS analysis, where the significant compounds were 4-phenyl-3-buten-2-ol (20.54%) and (Z)-3-butylidenephthalide (20.54%) that correspond to phthalide monomers. These results demonstrate that nanoemulsion of the volatile oil from L. porteri (at 10 and 30 mg/kg) shows a significant gastroprotective effect (72 and 92% respectively) against indomethacin-induced gastric damage, which is not different from the gastroprotective effect of omeprazole (69%) (p
- Published
- 2020
19. Hydroethanolic extract from Endopleura uchi (Huber) Cuatrecasas and its marker bergenin: Toxicological and pharmacokinetic studies in silico and in vivo on zebrafish
- Author
-
Helison de Oliveira Carvalho, Brenda Lorena Sánchez-Ortiz, Karyny Roberta Tavares Picanço, Luciane B. Silva, Rodrigo Cardoso Ataíde, Raphaelle Sousa Borges, Andrés Navarrete, José Carlos Tavares Carvalho, Cleydson B. R. Santos, Gloria Melisa Gonzalez Anduaga, Larissa Daniele Machado Góes, Beatriz Martins de S a Hyacienth, and Arlindo César Matias Pereira
- Subjects
HBA, Hydrogen bonding acceptors ,EEu, Endopleura uchi stem bark hydroethanolic extract ,IAN, Regional Herbarium of the Eastern Amazonian Embrapa ,PPB, Plasma protein binding ,Health, Toxicology and Mutagenesis ,In silico ,010501 environmental sciences ,Pharmacology ,Toxicology ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Pharmacokinetics ,In vivo ,lcsh:RA1190-1270 ,Biotrasformation ,Bergenin ,BBB, Brain-blood partition coefficient (C.brain/C.blood) ,0105 earth and related environmental sciences ,ComputingMethodologies_COMPUTERGRAPHICS ,lcsh:Toxicology. Poisons ,Hepatoxity ,CYP3A4 ,Chemistry ,ALT, Alanine aminotransferase ,hERG, ether-a-go-related human gene ,Regular Article ,MM, Molecular mass ,Acute toxicity ,HIA, Human intestinal absorption ,HAI, Index of Histopathological Changes ,Phytochemical ,Toxicity ,HBD, Hydrogen bonding donors ,Endopleura uchi ,P-Gp, P-glycoprotein ,Nephrotoxity ,AST, Aspartate aminotransferase ,030217 neurology & neurosurgery - Abstract
Graphical abstract, Highlights • E. uchi stem bark hydroethanolic extract in zebrafish. • Evaluating the in silico pharmacokinetic and toxicological parameters. • Behavioral, biochemical and histopathological changes was dose dependent. • In silico bergenin and its metabolites showed high intestinal absorption. • Bergenin inhibited CYP2C9, CYP3A4 and CYP2C19., Endopleura uchi, is used for the treatment of inflammatory disease and related to the female reproductive tract. The aim of this study was to evaluate the acute toxicity of the Endopleura uchi stem bark hydroethanolic extract (EEu) in zebrafish, emphasizing the histopathological and biochemical parameters, as well as evaluating the in silico pharmacokinetic and toxicological parameters of the phytochemical/pharmacological marker, bergenin, as their metabolites. The animals were orally treated with EEu at a single dose of 75 mg/kg, 500 mg/kg, 1000 mg/kg and 3000 mg/kg. the oral LD50 of the EEu higher to the dose of 3000 mg/kg. Behavioral, biochemical and histopathological changes were dose dependent. In silico pharmacokinetic predictions for bergenin and its metabolites showed moderate absorption in high human intestinal absorption (HIA) and Caco-2 models, reduced plasma protein binding, by low brain tissue binding and no P-glycoprotein (P-Gp) inhibition. Their metabolism is defined by the CYP450 enzyme, in addition to bergenin inhibition of CYP2C9, CYP3A4 and CYP2C19. In the bergenin and its metabolites in silico toxicity test it have been shown to cause carcinogenicity and a greater involvement of the bergenin with the CYP enzymes in the I and II hepatic and renal metabolism’s phases was observed. It is possible to suggest that the histopathological damages are involved with the interaction of this major compound and its metabolites at the level of the cellular-biochemical mechanisms which involve the absorption, metabolization and excretion of these possible prodrug and drug.
- Published
- 2020
20. Potential of the Compounds from
- Author
-
Mateus Alves, Batista, Abrahão Victor Tavares, de Lima Teixeira Dos Santos, Aline Lopes, do Nascimento, Luiz Fernando, Moreira, Indira Ramos Senna, Souza, Heitor Ribeiro, da Silva, Arlindo César Matias, Pereira, Lorane Izabel, da Silva Hage-Melim, and José Carlos Tavares, Carvalho
- Subjects
Molecular Docking Simulation ,Plant Extracts ,Tocotrienols ,Bixaceae ,Diterpenes ,Carotenoids ,Dyslipidemias - Abstract
Some significant compounds present in annatto are geranylgeraniol and tocotrienols. These compounds have beneficial effects against hyperlipidemia and chronic diseases, where oxidative stress and inflammation are present, but the exact mechanism of action of such activities is still a subject of research. This study aimed to evaluate possible mechanisms of action that could be underlying the activities of these molecules. For this, in silico approaches such as ligand topology (PASS and SEA servers) and molecular docking with the software GOLD were used. Additionally, we screened some pharmacokinetic and toxicological parameters using the servers PreADMET, SwissADME, and ProTox-II. The results corroborate the antidyslipidemia and anti-inflammatory activities of geranylgeraniol and tocotrienols. Notably, some new mechanisms of action were predicted to be potentially underlying the activities of these compounds, including inhibition of squalene monooxygenase, lanosterol synthase, and phospholipase A
- Published
- 2022
21. Potential of the compounds from Bixa orellana purified annatto oil and its granules (Chronic®) against dyslipidemia and inflammatory diseases: in silico studies with geranylgeraniol and tocotrienols
- Author
-
Mateus Alves Batista, Abrahão Victor Tavares de Lima Teixeira dos Santos, Aline Lopes do Nascimento, Luiz Fernando Moreira, Indira Ramos Senna Souza, Heitor Ribeiro da Silva, Arlindo César Matias Pereira, Lorane Izabel da Silva Hage-Melim, and José Carlos Tavares Carvalho
- Subjects
Chemistry (miscellaneous) ,Organic Chemistry ,Drug Discovery ,Molecular Medicine ,Pharmaceutical Science ,Physical and Theoretical Chemistry ,MALVALES ,Bixa orellana ,oil ,inflammatory process ,geranylgeraniol ,tocotrienol ,Analytical Chemistry - Abstract
Some significant compounds present in annatto are geranylgeraniol and tocotrienols. These compounds have beneficial effects against hyperlipidemia and chronic diseases, where oxidative stress and inflammation are present, but the exact mechanism of action of such activities is still a subject of research. This study aimed to evaluate possible mechanisms of action that could be underlying the activities of these molecules. For this, in silico approaches such as ligand topology (PASS and SEA servers) and molecular docking with the software GOLD were used. Additionally, we screened some pharmacokinetic and toxicological parameters using the servers PreADMET, SwissADME, and ProTox-II. The results corroborate the antidyslipidemia and anti-inflammatory activities of geranylgeraniol and tocotrienols. Notably, some new mechanisms of action were predicted to be potentially underlying the activities of these compounds, including inhibition of squalene monooxygenase, lanosterol synthase, and phospholipase A2. These results give new insight into new mechanisms of action involved in these molecules from annatto and Chronic®.
- Published
- 2022
22. A herbal oil in water nano-emulsion prepared through an ecofriendly approach affects two tropical disease vectors
- Author
-
Raimundo Nonato Picanço Souto, Raquel Silva Araújo, Rosa Helena Veras Mourão, Ricardo Marcelo dos Anjos Ferreira, Caio P. Fernandes, Rodrigo A.S. Cruz, José Carlos Tavares Carvalho, Anna E.M.F.M. Oliveira, and Jonatas Lobato Duarte
- Subjects
Larvicide ,biology ,Chemotype ,Verbenaceae ,lcsh:RS1-441 ,Aedes aegypti ,biology.organism_classification ,Citral ,Culex quinquefasciatus ,Miniemulsion ,lcsh:Pharmacy and materia medica ,Oil in water ,chemistry.chemical_compound ,chemistry ,Food science ,General Pharmacology, Toxicology and Pharmaceutics ,Colloidal system ,Droplet size distribution ,Lippia alba - Abstract
Lippia alba (Mill.) N.E.Br. ex Britton & P. Wilson, Verbenaceae, is considered a great source of a bioactive volatile oil. Due to the wide range of known chemotypes, its chemical analysis is very important. Among the several activities of this volatile oil, a potential larvicidal action against Culicidae species is highlighted. However, the low water miscibility of volatile oils limits their application in aqueous media. Oil in water nano-emulsions are in the spotlight of novelty to solve this main problem. Thus, the aim of the present study was to obtain this nanostructured system with L. alba volatile oil (citral chemotype) and evaluate its larvicidal activity against Aedes aegypti and Culex quinquefasciatus larvae. The major compounds were geranial (30.02%) and neral (25.26%). Low mean droplet size (117.0 ± 1.0 nm) and low polydispersity index (0.231 ± 0.004) were observed and no major changes were observed after seven days of storage. LC50 values against C. quinquefasciatus and A. aegypti third-instar larvae were respectively 38.22 and 31.02 ppm, while LC90 values were, respectively, 59.42 and 47.19 ppm. The present study makes use of a low energy, solvent-free and ecofriendly method with reduced costs. Thus, this paper contributes significantly to phyto-nanobiotechnology of larvicidal agents, opening perspectives for the utilization of L. alba volatile oil in integrated practices of vector control. Keywords: Colloidal system, Droplet size distribution, Miniemulsion, Larvicide
- Published
- 2019
23. Chromatographic profile, in silico and in vivo study of the pharmacokinetic and toxicological properties of major constituent present in kefir, the kefiran
- Author
-
Susy Érika de Lima Barros, Henrique Barros de Lima, Leandra Karoline Alves Gonçalves, Lenir Cabral Correia, Maiara de Fátima de Brito Brito, Mariana Pegrucci Barcelos, Guilherme Martins Silva, Carlos Henrique Tomich de Paula da Silva, Rafael Garrett da Costa, Rodrigo Alves Soares Cruz, José Carlos Tavares Carvalho, and Lorane Izabel da Silva Hage-Melim
- Subjects
Paper ,Health, Toxicology and Mutagenesis ,Toxicology - Abstract
Kefiran is a polysaccharide present in kefir grains that have been widely explored due to its potential health benefits. The objective of this work was to characterize and quantify the components present in the ethanolic extract of milk kefir grains; to study its pharmacokinetic and toxicological properties in silico and evaluate the acute toxicity of the kefiran in zebrafish. The prediction of pharmacokinetic properties was performed by QikProp software. In silico toxicity assessment was performed using the DEREK (deductive estimate of risk from existing knowledge) software. In the chromatographic, kefiran was identified as the major component. Results showed that the kefiran had low human oral absorption and intestinal absorption its due poor solubility profile; low logP value, indicating its lipophilicity and the low MDCK and Caco-2 cells permability, and unable to cross the blood–brain barrier. Kefiran did not present any structural warning for in silico toxicity. In zebrafish, the dose of 2,000 mg/kg of kefiran produced nonsignificant alterations in the analyzed organs. It can be said then that kefiran has an acceptable degree of safety for use in the development of drugs or functional foods. Further research such as in vivo testing to confirm its pharmacological potential is currently underway.
- Published
- 2021
24. Acute and Reproductive Toxicity Evaluation of Ormona® SI and Ormona® RC—Two New Nutraceuticals with Geranylgeraniol, Tocotrienols, Anthocyanins, and Isoflavones—In Adult Zebrafish
- Author
-
Clarice Flexa da Rocha, Camila de Nazaré Nunes Flexa, Gisele Custodio de Souza, Arlindo César Matias Pereira, Helison de Oliveira Carvalho, Aline Lopes do Nascimento, Natasha Juliana Perdigão de Jesus Vasconcelos, Heitor Ribeiro da Silva, and José Carlos Tavares Carvalho
- Subjects
Drug Discovery ,Pharmaceutical Science ,Molecular Medicine - Abstract
The zebrafish is a popular organism to test the toxicity of compounds. Here, we evaluate the acute and reproductive toxicity of Ormona SI® (OSI) and RC® (ORC), two herbal products developed for menopausal women with tocotrienols, geranylgeraniol, isoflavones, and anthocyanins. The acute toxicity was evaluated by behavioral alterations, lethality, and tissue changes (intestine, liver, kidney) after oral treatment with high product doses (500, 1000, and 2000 mg/kg). The reproductive toxicity was evaluated after 21 days of oral treatment with OSI and ORC at 200 mg/kg. Our results show that the LD50 could not be assessed due to the low mortality rate even with the highest dose; the behavioral alterations were not different from those of the group treated only with the vehicle (2% DMSO). The tissue changes were minor in OSI and more severe in ORC at the highest (2000 mg/kg) dose, while no tissue abnormality was observed at 500 mg/kg. In the reproductive assessment, continuous treatment could decrease the maturation of the reproductive cells, which also significantly decreases the egg spawning. This effect was attributed to the estrogenic activity of the isoflavones. In conclusion, the acute toxicity analysis shows that the products did not elicit lethal or sublethal effects observed in the model when used up to 500 mg/kg. Regarding the reproductive toxicity, decreased fertility was observed, which was expected due to the presence of isoflavones (phytoestrogens). Considering that the product is intended for menopausal and postmenopausal women, the presence of isoflavones is beneficial. Further studies should be performed to corroborate these results in mammals.
- Published
- 2022
25. Acute Toxicity and Anti-Inflammatory Activity of Trattinnickia rhoifolia Willd (Sucuruba) Using the Zebrafish Model
- Author
-
Agerdânio Andrade de Souza, Brenda Lorena Sánchez Ortíz, Swanny Ferreira Borges, Andria Vanessa Pena Pinto, Ryan da Silva Ramos, Igor Colares Pena, Rosemary de Carvalho Rocha Koga, Carla Estefani Batista, Gisele Custódio de Souza, Adriana Maciel Ferreira, Sergio Duvoisin Junior, and José Carlos Tavares Carvalho
- Subjects
Chemistry (miscellaneous) ,Organic Chemistry ,Drug Discovery ,Molecular Medicine ,Pharmaceutical Science ,ethnomedicine ,Trattinnickia ,anti-inflammatory ,preclinical trials ,molecular docking ,Physical and Theoretical Chemistry ,Analytical Chemistry - Abstract
The species Trattinnickia rhoifolia Willd, (T. rhoifolia), which belongs to the Burseraceae family, is widely used in ethnopharmacological cultural practices by traditional Amazonian people for anti-inflammatory purposes, sometimes as their only therapeutic resource. Although it is used in teas, infusions, macerations and in food, the species is still unexplored in regard to its pharmacophoric potential and chemical profile. Therefore, the aim of this study was to conduct a phytochemical characterization of the hydroethanolic extract of T. rhoifolia leaves (HELTr) and to evaluate the acute toxicity and anti-inflammatory activity of this species using zebrafish (Danio rerio). The extract was analyzed by gas chromatography–mass spectrometry (GC-MS). The evaluation of the acute toxicity of the HELTr in adult zebrafish was determined using the limit test (2000 mg/kg), with behavioral and histopathological evaluations, in addition to the analysis of the anti-inflammatory potential of HELTr in carrageenan-induced abdominal edema, followed by the use of the computational method of molecular docking. The phytochemical profile of the species is chemically diverse, suggesting the presence of the fatty acids, ester, alcohol and benzoic acid classes, including propanoic acid, ethyl ester and hexadecanoic acid. In the studies of zebrafish performed according to the index of histopathological changes (IHC), the HELTr did not demonstrate toxicity in the behavioral and histopathological assessments, since the vital organs remained unchanged. Carrageenan-induced abdominal edema was significantly reduced at all HELTr doses (100, 200 and 500 mg/kg) in relation to the negative control, dimethyl sulfoxide (DMSO), while the 200 mg/kg dose showed significant anti-inflammatory activity in relation to the positive control (indomethacin). With these activities being confirmed by molecular docking studies, they showed a good profile for the inhibition of the enzyme Cyclooxygenase-2 (COX-2), as the interactions established at the sites of the receptors used in the docking study were similar to the controls (RCX, IMN and CEL). Therefore, the HELTr has an acceptable degree of safety for acute toxicity, defined in the analysis of behavioral changes, mortality and histopathology, with a significant anti-inflammatory action in zebrafish at all doses, which demonstrates the high pharmacophoric potential of the species. These results may direct future applications and drug development but still require further elucidation.
- Published
- 2022
26. Co-Treatment of Purified Annatto Oil (Bixa orellana L.) and Its Granules (Chronic®) Improves the Blood Lipid Profile and Bone Protective Effects of Testosterone in the Orchiectomy-Induced Osteoporosis in Wistar Rats
- Author
-
Karyny Roberta Tavares Picanço, Maira Tiyomi Sacata Tongo Nazima, Abrahão Victor Tavares de Lima Teixeira dos Santos, Francinaldo Sarges Braga, Danna Emanuelle Santos Gonçalves, Helison de Oliveira Carvalho, Roberto Messias Bezerra, Alessandro de Sousa Nunes, José Carlos Tavares Carvalho, Talisson Taglialegna, Júlia Gomes Cerqueira, Janayra Maris Teixeira, Arlindo César Matias Pereira, and Heitor Ribeiro da Silva
- Subjects
Male ,medicine.medical_specialty ,Osteoporosis ,Pharmaceutical Science ,chemistry.chemical_element ,Blood lipids ,Drinking Behavior ,Calcium ,Protective Agents ,bone ,Article ,Analytical Chemistry ,QD241-441 ,Internal medicine ,Drug Discovery ,Androgen deficiency ,medicine ,Animals ,Plant Oils ,Femur ,Testosterone ,Orchiectomy ,Physical and Theoretical Chemistry ,Rats, Wistar ,achiote ,calcium ,androgen deficiency ,Plant Extracts ,Organic Chemistry ,Body Weight ,aging ,Bixaceae ,Feeding Behavior ,medicine.disease ,Carotenoids ,Lipids ,Endocrinology ,chemistry ,Chemistry (miscellaneous) ,Molecular Medicine ,Alkaline phosphatase - Abstract
This study aimed to evaluate and compare the effects of co-treatment with purified annatto oil (PAO) or its granules (GRA, Chronic®) with that of testosterone on the orchiectomy-induced osteoporosis in Wistar rats. After surgery, rats were treated from day 7 until day 45 with testosterone only (TES, 7 mg/kg, IM) or TES + PAO or GRA (200 mg/kg, p.o.). The following parameters were evaluated: food/water intake, weight, HDL, LDL, glucose, triglycerides (TG), total cholesterol (TC), alkaline phosphatase levels, blood phosphorus and calcium contents, femur weight, structure (through scanning electron microscopy), and calcium content (through atomic absorption spectrophotometry). Our results show that orchiectomy could significantly change the blood lipid profile and decrease bone integrity parameters. Testosterone reposition alone could improve some endpoints, including LDL, TC, bone weight, and bone calcium concentration. However, other parameters were not significantly improved. Co-treatment with PAO or GRA improved the blood lipid profile and bone integrity more significantly and improved some endpoints not affected by testosterone reposition alone (such as TG levels and trabeculae sizes). The results suggest that co-treatment with annatto products improved the blood lipid profile and the anti-osteoporosis effects of testosterone. Overall, GRA had better results than PAO.
- Published
- 2021
- Full Text
- View/download PDF
27. Hypoglycemic Activity of Aqueous Extract of Latex from Hancornia speciosa Gomes: A Study in Zebrafish and In Silico
- Author
-
Arlindo César Matias Pereira, José Carlos Tavares Carvalho, Diego Quaresma Ferreira, Gisele Custódio de Souza, Sheylla Susan Moreira da Silva de Almeida, Wandson Braamcamp de Souza Pinheiro, Henrique Barros de Lima, Ângela Costa Figueira, José Rodrigues Pinheiro Neto, Rosana Tomazi, Adriana Maciel Ferreira, Geilson Alcantara da Silva, and Lorane Izabel da Silva Hage-Melim
- Subjects
DIABETES MELLITUS ,Pharmaceutical Science ,Pharmacology ,Bornesitol ,chemistry.chemical_compound ,Pharmacy and materia medica ,1-O-methyl-myoinositol ,In vivo ,Diabetes mellitus ,Drug Discovery ,medicine ,diabetes ,Metabolism ,medicine.disease ,zebrafish ,Acute toxicity ,Metformin ,RS1-441 ,chemistry ,Phytochemical ,Toxicity ,Hancornia speciosa Gomes ,Molecular Medicine ,Medicine ,medicine.drug - Abstract
Hancornia speciosa Gomes is a tree native to Brazil and has therapeutic potential for several diseases. Ethnopharmacological surveys have reported that the plant is used as a hypoglycemic agent and to lose weight. This study aimed to evaluate the effects of the aqueous extract from H. speciosa latex (LxHs) in a zebrafish model of diabetes. The extract was evaluated through high-performance thin-layer chromatography (HTPLC), nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy (FT-IR). We then tested treatments with LxHs (500, 1000, and 1500 mg/kg) by assessing blood glucose levels in alloxan-induced diabetic animals, and metformin was used as a control. The toxicity was evaluated through histopathology of the pancreas and biochemical assessment of serum levels of AST, ALT, creatinine, and urea. The extract was also assessed for acute toxicity through several parameters in embryos and adult animals. Finally, we performed in silico analysis through the SEA server and docking using the software GOLD. The phytochemical study showed the compounds cornoside, dihydrocornoide, and 1-O-methyl-myoinositol (bornesitol). The treatment with all doses of LxHs significantly decreased alloxan-induced hyperglycemia without any significant histological or biochemical abnormalities. No significant frequency of teratogenesis was observed in the embryos exposed to the extract, and no significant behavioral changes or deaths were observed in adult animals. In silico, the results showed a potential interaction between inositol and enzymes involved in carbohydrates’ metabolism. Overall, the results show a hypoglycemic activity of the extract in vivo, with no apparent toxicity. The computational studies suggest this could be at least partially due to the presence of bornesitol, since inositols can interact with carbohydrates’ enzymes.
- Published
- 2021
28. Hypoglycemic Activity of Aqueous Extract of Latex from
- Author
-
Rosana, Tomazi, Ângela Costa, Figueira, Adriana Maciel, Ferreira, Diego Quaresma, Ferreira, Gisele Custódio, de Souza, Wandson Braamcamp, de Souza Pinheiro, José Rodrigues, Pinheiro Neto, Geilson Alcantara, da Silva, Henrique Barros, de Lima, Lorane Izabel, da Silva Hage-Melim, Arlindo César Matias, Pereira, José Carlos Tavares, Carvalho, and Sheylla Susan Moreira, da Silva de Almeida
- Subjects
diabetes ,zebrafish ,1-O-methyl-myoinositol ,Article ,Hancornia speciosa Gomes - Abstract
Hancornia speciosa Gomes is a tree native to Brazil and has therapeutic potential for several diseases. Ethnopharmacological surveys have reported that the plant is used as a hypoglycemic agent and to lose weight. This study aimed to evaluate the effects of the aqueous extract from H. speciosa latex (LxHs) in a zebrafish model of diabetes. The extract was evaluated through high-performance thin-layer chromatography (HTPLC), nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy (FT-IR). We then tested treatments with LxHs (500, 1000, and 1500 mg/kg) by assessing blood glucose levels in alloxan-induced diabetic animals, and metformin was used as a control. The toxicity was evaluated through histopathology of the pancreas and biochemical assessment of serum levels of AST, ALT, creatinine, and urea. The extract was also assessed for acute toxicity through several parameters in embryos and adult animals. Finally, we performed in silico analysis through the SEA server and docking using the software GOLD. The phytochemical study showed the compounds cornoside, dihydrocornoide, and 1-O-methyl-myoinositol (bornesitol). The treatment with all doses of LxHs significantly decreased alloxan-induced hyperglycemia without any significant histological or biochemical abnormalities. No significant frequency of teratogenesis was observed in the embryos exposed to the extract, and no significant behavioral changes or deaths were observed in adult animals. In silico, the results showed a potential interaction between inositol and enzymes involved in carbohydrates’ metabolism. Overall, the results show a hypoglycemic activity of the extract in vivo, with no apparent toxicity. The computational studies suggest this could be at least partially due to the presence of bornesitol, since inositols can interact with carbohydrates’ enzymes.
- Published
- 2021
29. Development of an environmentally friendly formulation of silk fibroin associated with fatty acid from Astrocaryum murumuru Mart. fat, effective against larvae of the Aedes aegypti vector
- Author
-
Ricardo Marcelo dos Anjos Ferreira, Abrahão Victor Tavares de Lima Teixeira dos Santos, Fernando B. Neves, Irlon M. Ferreira, Raimundo Nonato Pincaço Souto, David E. Q. Jimenez, Victor H.S. Marinho, José Carlos Tavares Carvalho, Fábio Rodrigues de Oliveira, and Sergio A. Yoshioka
- Subjects
chemistry.chemical_classification ,Astrocaryum murumuru ,Larva ,chemistry ,biology ,Vector (epidemiology) ,Fatty acid ,Fibroin ,Food science ,Aedes aegypti ,biology.organism_classification ,Environmentally friendly - Abstract
The Aedes aegypti mosquito is a vector of several diseases, such as dengue, malaria and the Zika virus. Synthetic insecticides such as chlorpyrifos and chlorothalonil have been used for plague control, despite causing damage to the environment and to humans. It is therefore important to study natural active compounds with a low environmental impact. The present paper developed an environmentally friendly formulation of silk fibroin (SF) associated with fatty acid esters [ethyl (FAEE-SF), propyl (FAPE-SF) and butyl (FABE-SF)] from Astrocaryum murumuru Mart. fat, which was effective against Aedes aegypti 3th instar larvae. The FABE-SF nanoemulsion induced a higher mortality rate in the larvae of the A. aegypti after 48 h (LC50 = 18.92 µg / mL). The stabilities of the nanoemulsions were monitored for 21 days, and FABE-SF exhibited greater stability throughout the monitored period, with average particle, zeta and PDI values of around 217 ± 0.85 nm, -25.6 ± 3.24 mV and 0.338 ± 0.01, respectively. This paper reported the first effective action of fatty acid esters from A. murumuru Mart. associated with silk fibroin against A. aegypti. The FABE-SF solution also had a low hemolytic index, suggesting that treatment may be safe for animal and human use.
- Published
- 2021
30. Fatty Ethanolamide of Bertholletia Excelsa Triglycerides (Brazil nuts): Anti-Inflammatory Action and Acute Toxicity Evaluation in Zebrafish (Danio rerio)
- Author
-
Gisele Custódio de Souza, Mateus Alves Batista, José Carlos Tavares Carvalho, Irlon M. Ferreira, Brenda Lorena Sánchez-Ortiz, Raphaelle Sousa Borges, Yesica Fernanda Quitian-Useche, Lorane Izabel da Silva Hage Melim, Swanny Ferreira Borges, and Benilson Ramos
- Subjects
Polyunsaturated Alkamides ,medicine.drug_class ,Immunology ,Anti-Inflammatory Agents ,Danio ,Pharmacology ,Carrageenan ,Anti-inflammatory ,Structure-Activity Relationship ,chemistry.chemical_compound ,food ,In vivo ,Toxicity Tests, Acute ,medicine ,Animals ,Edema ,Ethanolamide ,Pharmacology (medical) ,Zebrafish ,chemistry.chemical_classification ,Dose-Response Relationship, Drug ,biology ,biology.organism_classification ,food.food ,Acute toxicity ,Molecular Docking Simulation ,Enzyme ,chemistry ,Toxicity ,Bertholletia ,Brazil nut - Abstract
Fatty amides (N-alkylamides) are a group of bioactive lipids widely distributed in microorganisms, animals, and plants. The low yield in the extraction process of spilantol, a grease amide, which has been related mainly to diverse biological effects, compromises its application on a large scale. Thus, this study proposed an alternative to the synthesis of fatty amides from Bertholletia excelsa (AGBe) oil, with a chemical structure similar to that of spilantol. In vivo models induced by carrageenan were used in Zebrafish (Danio rerio). In in vivo studies, oral AGBe produced no signs of toxicity. In the histopathological study, AGBe did not cause significant changes in the main metabolizing organs (liver, kidneys, and intestines). In the anti-inflammatory evaluation, all doses (45 mg/kg, 500 mg/kg, and 1000 mg/kg) were effective, significantly reducing edema and producing a dose-response effect when compared to spilantol. In the in silico study, with the use of molecular docking, he showed that among the AGBe, the molecules 18:1, ω-7-ethanolamine and 18:1, ω-9-ethanolamine stood out, which had 21 interactions for COX-2 and 20 interactions for PLA2, respectively, surpassing the spilantol standard with 15 interactions for COX-2 and PLA2. The hypothesis of anti-inflammatory action was confirmed in the in silico study, demonstrating the involvement of AGBe in the process of inhibiting the enzymes COX-2 and PLA2. Therefore, based on all the results obtained and the fact that until the dose of 1000 mg/kg, orally, in zebrafish, it was not possible to determine the LD50, it can be said that AGBe is effective and safe for the activity anti-inflammatory.
- Published
- 2021
31. Obtaining and characterization of anthocyanins from Euterpe oleracea (açaí) dry extract for nutraceutical and food preparations
- Author
-
Abrahão Victor Tavares de Lima Teixeira dos Santos, Jesús Rafael Rodríguez Amado, Helison de Oliveira Carvalho, Mayara Brito de Sousa, Heitor Ribeiro da Silva, José Otávio Carréra Silva Júnior, Ariadna Lafourcade Prada, Daniele da Cruz de Assis, José Carlos Tavares Carvalho, and Adriana Maciel Ferreira
- Subjects
Cadmium ,Residue (complex analysis) ,Chromatography ,010405 organic chemistry ,DPPH ,Açai ,Extraction (chemistry) ,chemistry.chemical_element ,lcsh:RS1-441 ,Extraction ,Zinc ,Factorial experiment ,01 natural sciences ,0104 chemical sciences ,Anthocyanins ,lcsh:Pharmacy and materia medica ,010404 medicinal & biomolecular chemistry ,Acetic acid ,chemistry.chemical_compound ,chemistry ,Anthocyanin ,Dry extract ,Factorial design ,General Pharmacology, Toxicology and Pharmaceutics ,Antioxidant - Abstract
The main constituents of the Euterpe oleracea Mart., Arecaceae, fruits (açaí) are anthocyanins. This paper aimed to standardize the extraction process and characterize an anthocyanin-rich dry extract obtained from this fruit. A 23 full factorial design was used. The volumes of ethanol 92% and acetic acid and the extraction time were used as factors. Total solids and anthocyanins content were used as feedback. The dry extract was obtained by freeze-drying. The content of anthocyanins was determined spectrophotometrically. Fourier Transform Infrared Spectroscopy, Differential Scanning Calorimeter, Thermogravimetry, Scanning Electron Microscopy, and Atomic Absorption Spectrometry were used for characterizingthe dry extract. The DPPH method was used for evaluating radical scavenging activity. The extraction conditions were established. The most influent factor was the volume of acetic acid. The dry extract moisture content was equal to 1.39 ± 0.25%, the evaporation residue 97.25 ± 1.28%, total ashes 0.62 ± 0.12%, and the anthocyanin content was 61.75 ± 3.28%. The elemental composition shows the presence of manganese 4.85 ppm, iron 1.62 ppm, zinc 0.05, copper 1.38 ppm, calcium 1.01 ppm, cadmium 0.003 ppm, nickel 0.37 ppm, and lead 0.38 ppm. The dried extract IC50 estimated by the radical scavenging assay with DPPH was 31.25 ± 2.31 ppm. The optimal extraction conditions were: the volume of ethanol 92%: 400 ml; volume of acetic acid: 75 ml; an extraction time: 4 h. Keywords: Açai, Anthocyanins, Antioxidant, Dry extract, Extraction, Factorial design
- Published
- 2019
32. The Use of Medicinal Plants in the Riverside Community of the Mazagão River in the Brazilian Amazon, Amapá, Brazil: Ethnobotanical and Ethnopharmacological Studies
- Author
-
Raullyan Borja Lima E Silva, Gabriel Araújo da Silva, Ícaro Rodrigues Sarquis, Mário Augusto Gonçalves Jardim, José Carlos Tavares Carvalho, Iann Rodrigues Sarquis, Rosângela do Socorro Ferreira Rodrigues Sarquis, Brenda Lorena Sánchez-Ortiz, and Caio P. Fernandes
- Subjects
0303 health sciences ,Carapa guianensis ,Euterpe ,Article Subject ,biology ,Traditional medicine ,ved/biology ,Virola surinamensis ,ved/biology.organism_classification_rank.species ,Introduced species ,lcsh:Other systems of medicine ,Arecaceae ,lcsh:RZ201-999 ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,03 medical and health sciences ,Geography ,Complementary and alternative medicine ,Ethnobotany ,Arrabidaea chica ,Hura ,Research Article ,030304 developmental biology - Abstract
The inhabitants of the floodplain of the Mazagão River in the State of Amapá in the Brazilian Amazon have inherited from indigenous African and Cabocla cultures indications for the use and forms of preparation of medicinal plants to cure diseases of the body and spirit. This study aimed to perform an ethnopharmacological survey of medicinal plants used by the riparian community of the floodplains of the Mazagão River, in the State of Amapá. In this study, we chose semistructured interviews with socioeconomic, ethnopharmacological, and ethnobotanical aims. The collection of medicinal plants occurred during guided tours. The Use Value (UV), Informant Consensus Factor (ICF), Correction Factor (CF), and Fidelity level (FL) were calculated. There were 130 species of medicinal plants, distributed in 116 genera and 57 families; Fabaceae (16), Lamiaceae (14), Euphorbiaceae (7), and Arecaceae (6) include 33.33% of the total species sampled. All 95 native species of floodplain forests were previously described, and 35 are exotic species. The species with the highest UV (≥ 0.5) at the mouth of the Mazagão River were Carapa guianensis (0.91), Pentachlethra macroloba (0.83), Dalbergia subcymosa (0.77), Uncaria tomentosa (0.75), Otacanthus azureus (0.62), Virola surinamensis (0.62), Hura crepitans (0.58), Euterpe oleracea (0.56), and Arrabidaea chica (0.51). These species were also the ones that presented the highest ICF among the informants and 100% in FL for a specific therapeutic use. The study is comprised of 16 categories of therapeutic use, of which the majority of the plants used are related to diseases such as microbial infections (20.67%, 73 species), gastrointestinal disorders (13.31%), and inflammation (11.61%). The results showed that knowledge about the use of medicinal plants along the rivers and streams that form the mouth of the Mazagão River is evenly distributed. Most of the interviewees present diversified knowledge about the medicinal resources because they have a close relationship with the floodplain forest. Native species of this forest predominate among the most commonly used medicinal plants as subsidies for future pharmacological studies.
- Published
- 2019
33. Mosquiticidal and repellent potential of formulations containing wood residue extracts of a Neotropical plant, Tabebuia heptaphylla
- Author
-
Khalid Haddi, Jaqueline Cibene Moreira Borges, Julcemar Didonet, Alex Sander Rodrigues Cangussu, José Carlos Tavares Carvalho, Eugênio E. Oliveira, Ilsamar Mendes Soares, Tarcisio Silva Melo, Sérgio Donizeti Ascêncio, Raimundo Wagner de Souza Aguiar, Bruno Silva Andrade, Vitor L. Nascimento, and Nádia Rezende Barbosa Raposo
- Subjects
0106 biological sciences ,Odorant binding ,Oviposition ,Purple ipê ,Bignoniaceae ,Aedes aegypti ,01 natural sciences ,Residue (chemistry) ,chemistry.chemical_compound ,Mosquitoes control ,Food science ,Repellence ,Lapachol ,biology ,010405 organic chemistry ,Chemistry ,fungi ,Plant extracts ,biology.organism_classification ,0104 chemical sciences ,Tabebuia ,Biopesticide ,Docking (molecular) ,Molecular docking ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
The use of wood residues and their extracts as sources for bioactive molecules not only helps to reduce the environmental impacts of the wastage produced by timber industry but also provide innumerous biotechnological application opportunities, including the development of novel insecticide products. Here, the insecticidal and repellent (in gel and cream formulations) potential of wood extracts of the Neotropical plant “purple ipe”, Tabebuia heptaphylla (Bignoniaceae) against the mosquitoes Aedes aegypti are investigated. Firstly, the chemical composition of the chloroform and hexane extracts obtained from T. heptaphylla residues is analyzed and their insecticidal activities are assessed against A. aegypti larvae. Furthermore, the repellence and oviposition deterrence actions of these extracts were evaluated against adult mosquito females. Computational docking analysis was also conducted to predict the physical interactions between the major constituents of the T. heptaphylla residue extracts and the odorant binding receptor of A. aegypti. Despite the fact that the 2,6-di-tert-butylnaphthalene was the major constituent (53.3%) in the T. heptaphylla hexanoic extract, the presence of lapachol, 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthalenedione, as major constituent in both wood extracts combined with the computational docking analysis, strongly suggest the lapachol as the major factor of the insecticidal/repellent effects of T. heptaphylla wood extracts. The computational docking analysis predicted significant binding of lapachol with the internal active pocket of the mosquito odorant binding receptor, which helps to explain the up to 3 h protection against bites of A. aegypti females provided by gel and cream formulations containing the T. heptaphylla residue extracts. Collectively, the findings demonstrated relevant biotechnological and environmentally-friend opportunities (i.e., production of biopesticides) for the wood residues produced by timber activities during the extraction and processing of T. heptaphylla trees.
- Published
- 2019
34. Cassia grandis Lf nanodispersion is a hypoglycemic product with a potent α-glucosidase and pancreatic lipase inhibitor effect
- Author
-
Emerson Silva Lima, Leonard D.R. Acho, Ariadna Lafourcade Prada, Tatiane Pereira de Souza, José Carlos Tavares Carvalho, Jesús Rafael Rodríguez Amado, Márcia de Jesus Amazonas da Silva, and Hady Keita
- Subjects
Antioxidant ,DPPH ,medicine.medical_treatment ,Pharmaceutical Science ,Pharmacology ,Article ,Glibenclamide ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Lipase ,IC50 ,030304 developmental biology ,0303 health sciences ,Cassia grandis ,ABTS ,biology ,lcsh:RM1-950 ,biology.organism_classification ,lcsh:Therapeutics. Pharmacology ,chemistry ,Alpha-glucosidase ,030220 oncology & carcinogenesis ,biology.protein ,medicine.drug - Abstract
Purpose: This study aimed to evaluate the hypoglycemic effect, antioxidant, α-glucosidase and lipase inhibitory activity, and the cytotoxicity of the Cassia grandis nanodispersion (CgND). Methods: The hypoglycemic effect was evaluated in alloxan-induced diabetic mice. The particle size, polydispersion index, ζ-potential, and conductivity, as well as the drug-loaded content, were monitored in shelf-live, along a year. The delivery profile was evaluated in simulated intestinal fluids at pH 6.5 and 7.4. The antioxidant effect was evaluated as DPPH and ABTS inhibition. The murine α-glucosidase inhibitory activity and the lipase-inhibitory effect were evaluated in vitro. Cytotoxicity was evaluated by the Alamar blue test. Results: CgND remained stable for a year in shelf conditions. The hypoglycemic effect in a dose of 10 mg/kg was not statistically different from glibenclamide 25 mg/kg. Nanoparticles released 100% of extract in 120 min at pH 6.5 and 7.4. Nanodispersion exhibited a potent α-glucosidase and lipase-inhibitory effect with IC50 of 3.96 and 0.58 µg/mL, respectively. A strong antioxidant activity against DPPH (IC50 0.65 µg/mL) and ABTS (0.48 µg/mL) was also observed. The hypoglycemic effect could occur, at least in part, via antioxidant and α-glucosidase inhibition. CgND is non-cytotoxic in MRC-5 line cell. This nanodispersion is a promising nanotechnological product that could be used in pharmaceuticals for the treatment of Type II diabetes and related complications as obesity. Keywords: Hypoglycemic, Nanodispersion, Cassia grandis, Lipase inhibitory, Alpha-glucosidase, Antioxidant
- Published
- 2019
35. Rosmarinus officinalis essential oil: A review of its phytochemistry, anti-inflammatory activity, and mechanisms of action involved
- Author
-
Arlindo César Matias Pereira, Brenda Lorena Sánchez Ortiz, Raphaelle Sousa Borges, José Carlos Tavares Carvalho, and Hady Keita
- Subjects
medicine.drug_class ,Phytochemicals ,Anti-Inflammatory Agents ,Salvia ,Rosmarinus ,Anti-inflammatory ,law.invention ,03 medical and health sciences ,Camphor ,chemistry.chemical_compound ,0302 clinical medicine ,law ,Drug Discovery ,Oils, Volatile ,medicine ,Animals ,Humans ,Essential oil ,030304 developmental biology ,Pharmacology ,0303 health sciences ,biology ,Traditional medicine ,biology.organism_classification ,chemistry ,Phytochemical ,030220 oncology & carcinogenesis ,Officinalis ,Lamiaceae ,Phytotherapy - Abstract
Ethnopharmacological relevance Plant species Rosmarinus officinalis L. (Lamiaceae; Synonyms: Salvia rosmarinus Schleid. and Rosmarinus angustifolius Mill.) is a herb widely used worldwide. In local and traditional medicine, its used for inflammation-related diseases. Currently, studies report anti-inflammatory activity in its essential oil (EORO). However, to better understand EORO’s anti-inflammatory activity its necessary to understand its phytochemistry and the signaling pathways affected by it. Hence, this review aimed to describe EORO phytochemical profile, ethnopharmacological uses, some biological activities of EORO will be described but emphasizing its anti-inflammatory potential and possible mechanisms of action involved. Materials and methods The research was performed using the databases Medline, Embase, BVS Regional Portal, Science Direct, CAPES Journals, and Scopus; using the keywords “Rosmarinus officinalis”, “anti-inflammatory” and “essential oil”. Additional information was gathered from related textbooks, reviews, and documents. Results and discussion Until now about 150 chemical compounds were identified in EORO samples, more frequently reported molecules were 1,8-cineole, α-pinene, and camphor. Studies suggest that the anti-inflammatory activity of EORO occur mainly through inhibition of NF-κB transcription and suppression of arachidonic acid cascade. Its antioxidant activity also aids by preventing injury caused by the reactive species of inflammation; its smooth muscle relaxant activity contributes to ameliorating airway inflammatory diseases. Lastly, toxicity assessments indicate low toxicity to EORO. Conclusions Current evidence indicates anti-inflammatory activity in EORO, supporting its ethnopharmacological uses in inflammatory-related diseases, and potential future applications. However, although considerable acute inflammatory models were tested, more chronic inflammatory models are needed; clinical studies are still absent, this may be due to the high doses needed for essential oils to exert pharmacological effects, but recent studies show this issue can be bypassed using the oil formulated as nanoemulsions to improve its bioavailability.
- Published
- 2019
36. Evidence for Involvement of TRPV1 Receptors and Potassium Channels in the Seizures Induced by α-Sanshool
- Author
-
Mario Noel Morales-Hernández, Andrés Navarrete, José Luis Balderas-López, José Carlos Tavares-Carvalho, Benito Reyes-Trejo, and Gloria Melisa González-Anduaga
- Subjects
TRPV1 ,Strychnine ,Bicuculline ,Pharmacology ,Potassium channel ,chemistry.chemical_compound ,Mechanism of action ,chemistry ,medicine ,Diazoxide ,medicine.symptom ,Pentylenetetrazol ,Capsazepine ,medicine.drug - Abstract
α-Sanshool is an alkamide isolated from the stem bark of Zanthoxylum liebmannianum, a Mexican medicinal plant known as Colopahtle. Our research group has reported that the intraperitoneal administration of α-sanshool induces tonic-clonic seizures in mice. In the present study, we investigated the convulsive effect of this alkamide and elucidated its mechanism of action by comparing with well-known convulsive and anticonvulsive drugs in an in vivo approach. α-Sanshool showed a potent (ED50 [CL 95%]=3.06 [2.92–3.22] mg/kg) and immediate (2±2 s) seizure effect after the intraperitoneal administration in mice. The convulsive effect of this alkamide was only observed for intraperitoneal administration; the oral route did not show any effect. α-Sanshool was less potent than strychnine (ED50 [CL 95%]=1.53 [1.44–1.62] mg/kg), but more effective than bicuculline, 4-aminopyridine, affinin, and pentylenetetrazol, in that order. The seizures induced by α-sanshool were reduced by capsazepine and diazoxide, suggesting the involvement of TRPV1 and potassium channels in the mechanism of action of this compound.
- Published
- 2019
37. Sphaerospora festivus n. sp., a parasite of the flag cichlid, Mesonauta festivus (Teleostei: Cichlidae) from eastern Amazon, Brazil
- Author
-
Roger Leomar da Silva Ferreira, Edilson Matos, José Carlos Tavares Carvalho, Diehgo Tuloza da Silva, Luana Silva Bittencourt, Marcela Nunes Videira, Igor Hamoy, and Marcelo Francisco da Silva
- Subjects
myxozoa ,Parasitic Diseases, Animal ,Zoology ,DNA, Ribosomal ,SF1-1100 ,Mesonauta festivus ,18S ribosomal RNA ,030308 mycology & parasitology ,Fish Diseases ,03 medical and health sciences ,Amazonia ,Genus ,Cichlid ,Animals ,Parasite hosting ,Parasites ,Phylogeny ,030304 developmental biology ,0303 health sciences ,Teleostei ,Myxozoa ,General Veterinary ,biology ,Phylogenetic tree ,Cichlids ,biology.organism_classification ,Animal culture ,18S rRNA ,Sphaerospora festivus ,Parasitology ,Brazil ,morphometry - Abstract
The present study describes a new species of the genus Sphaerospora found in the urinary bladder of the flag cichlid, Mesonauta festivus collected in Corre Água district of the municipality of Macapá, Amapá State (Brazil). The study includes morphological and phylogenetic analyses of the new parasite, to determine the relationship of the new species with related myxosporean species. The new species has polysporous plasmodia, which vary in size and shape. The mature myxospores are subspherical shape in valvar view. In the sutural view, the myxospores are 5.3±0.2 (5.2-5.6) μm in length and 7.0±0.7 (6.3-7.7) μm in width, with two piriform polar capsules equal size, 2.5±0.2 (2.3-2.8) μm in length and 1.8±0.2 (1.6-2.0) μm in width. The phylogenetic analyses of a partial sequence of the 18S rRNA gene confirmed the status of the new species and determined the relationship of the new species and related myxosporean species.The sum of the evidence indicates that, Sphaerospora festivus n. sp. belongs to the family Sphaerosporidae, and is the first record of the genus Sphaerospora from Brazil.
- Published
- 2021
38. Development of eco-friendly nano-mosquitocides against arboviruses vectors
- Author
-
Caio P. Fernandes, José Carlos Tavares Carvalho, Lisset Ortiz Zamora, and Anna E.M.F.M. Oliveira
- Subjects
business.industry ,fungi ,parasitic diseases ,Biology ,business ,Environmentally friendly ,Biotechnology - Abstract
Increasing efforts have been observed for the development of eco-friendly mosquitocides against arboviruses vectors. The diseases transmitted by some mosquitoes have been of global concern, being huge problems of public health in low-income countries and recent cases in developing countries opened an alert. The control may be performed in the mature form of the mosquitoes or the immature forms, such as larvae and pupae. Growing interest is observed for the nano-based formulations, especially those prepared with natural products. Therefore, this chapter shows recent advances through a revision of the literature and summary of the researches that bring the main nanoformulations and their respective bioactivities against mosquito vectors.
- Published
- 2021
39. Contributors
- Author
-
B.A. Aderibigbe, Jubril O. Akolade, R. Alex Christon, S. Alven, Ricardo Guimarães Amaral, Luciana Nalone Andrade, Maria Terezinha Bahia, Abideen O. Balogun, Mohammed O. Balogun, Leandro R.S. Barbosa, Raquel de Melo Barbosa, Caroline R. Basso, Melibea Berzosa, Marta Helena Branquinha, Gabriel Bezerra Motta Câmara, Juliana Cordeiro Cardoso, Kevin Silva Carvalhal, José Carlos Tavares Carvalho, Simone Santiago de Carvalho Oliveira, Bruna Renata Casadei, Gustavo R. Castro, Bruno P. Crulhas, Jorddy Neves Cruz, Maria do Socorro Pires e Cruz, Joyce Kelly Marinheiro da Cunha Gonsalves, Eugênio Fonseca da Silva Júnior, Antonio Pedro da Silva Souza Filho, Subramanyam Deepika, Judith Pessoa A. Feitosa, Caio Pinho Fernandes, Christine Lamenha Luna Finkler, Juliana Gouveia Galvão, Carlos Gamazo, Fátima García-Villén, Murugesan Gnanadesigan, Marcelo Gomes de Gomes, Gustavo González-Gaitano, Gopika Gopakumar Nageswari, Sandra Elisa Haas, Rajendran Harishkumar, Zaved Hazarika, Cesar Viseras Iborra, Juan M. Irache, V. Janarthanan, Anupam Nath Jha, Nikhilesh Joardar, Rangaraj Kiruthika, Mayra C.G. Lotierzo, Tamara Ramos Maciel, Barbara Malheiros, Ana Lia Mazzeti, Lyghia Maria Araújo Meirelles, Claudia Moura Melo, Selvaraj Mohana Roopan, Anupam Mondal, Naba Kumar Mondal, Vanessa Carla Furtado Mosqueira, Luana Dias de Moura, Niladri Mukherjee, R. Muthusamy, Leopoldo Fabrício Marçal do Nascimento, Paul Nguewa, Gabrielle Barrozo Novais, Anna Eliza Maciel de Faria Mota Oliveira, Douglas Santos Oliveira, Marcos Enê Chaves Oliveira, Mozaniel Santana de Oliveira, Yadira Pastor, Haroldo Cesar B. Paula, Regina Célia Monteiro de Paula, Valber A. Pedrosa, José Peña-Guerrero, Daniel Santiago Pereira, Joan Puig-Rigall, Fernanda Nervo Raffin, Sanchaita Rajkhowa, Ana Rosa Richter, Leandro Stefano Sangenito, Robertta Jussara Rodrigues Santana, André Luis Souza dos Santos, Chinnadurai Immanuel Selvaraj, Patrícia Severino, M.S. Shivakumar, Santi P. Sinha Babu, Eliana Maria Barbosa Souto, Aarthy C Vallur, G. Vengateswari, P. Vivekanandhan, K. Yashkamal, and Lisset Ortiz Zamora
- Published
- 2021
40. Evaluation of the in vitro release of isoflavones from soybean germ associated with kefir culture in the gastrointestinal tract and anxiolytic and antidepressant actions in zebrafish (Danio rerio)
- Author
-
Brenda Lorena Sánchez-Ortiz, Raimundo da Silva Barbosa, Iracirema da Silva Sena, Helison de Oliveira Carvalho, Abrahão Victor Tavares de Lima Teixeira, Aline Moreira Pinto, Ester Lopes de Melo, José Carlos Tavares Carvalho, Xuebo Hu, Camila Lins Bilby Baima, Heitor Ribeiro da Silva, and Arlindo César Matias Pereira
- Subjects
0301 basic medicine ,medicine.drug_class ,Medicine (miscellaneous) ,Regulation of gastric function ,Pharmacology ,Anxiety ,Anxiolytic ,Buspirone ,Brain-intestine axis ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Kefir ,medicine ,TX341-641 ,Gastrointestinal tract in vitro ,Gastrointestinal tract ,030109 nutrition & dietetics ,Nutrition and Dietetics ,Chemistry ,Depression ,Nutrition. Foods and food supply ,04 agricultural and veterinary sciences ,Isoflavones ,040401 food science ,Aglycone ,Phytoestrogens ,Caffeine ,Food Science ,medicine.drug - Abstract
The gastrointestinal tract contains thousands of microorganisms present in the mucosa, derived from several different species, and these microorganisms have a direct relationship with immunity, homeostasis, and certain systemic diseases. The microorganisms present in kefir grains interact with the gastrointestinal and immune systems as well as with the intestinal microbiota. Isoflavones are the most commonly consumed phytoestrogens, with aglycone and glycoside forms. Therefore, in this study, the content of aglycone isoflavones released from kefir associated with soybean germ (KSG) was evaluated in a gastrointestinal digestion model in vitro, and the anxiolytic and antidepressant activity of KSG and the fermentation solution of kefir (FSK) were evaluated in models of anxiety and depression in zebrafish (Danio rerio). KSG was prepared as the 4-day fermentation product of 40 g/L brown sugar, 10 g/L soy germ, and 60 g/L kefir. Isoflavone content was evaluated during the gastric, intestinal, and colon phases in simulated gastrointestinal digestion and analyzed by high-performance liquid chromatography. KSG was administered orally to zebrafish for 30 days, at 200 mg/kg (group G1) and 400 mg/kg (G2), while G3 received 2 μL SFK. The scototaxis test (light–dark) was performed to assess anxiolytic activity, using caffeine (100 mg/kg) as an anxiolytic, buspirone (25 mg/kg) as a positive control, and distilled water as a negative control. To assess antidepressant activity, a new tank dive test was performed, using 1% ethanol as a depressant, fluoxetine (20 mg/kg) as a positive control, and distilled water as a negative control. An increase in the release of isoflavones in the gastric phase and a decrease in the intestinal and colon phases was demonstrated with KSG. Moreover, the behavioral patterns observed in the different experiments demonstrated that KSG and FSK produce anxiolytic and antidepressant effects.
- Published
- 2020
41. Assessment of the hypoglycemic effect of Bixin in alloxan-induced diabetic rats: in vivo and in silico studies
- Author
-
Rodolfo Bortolozo Serafim, Silvana Giuliatti, Irlon M. Ferreira, Andrés Navarrete Castro, Matheus Mercês Ramos, Hady Keita, Cleydson B. R. Santos, José Carlos Tavares Carvalho, Gabriel Monteiro da Silva, Elias Carvalho Padilha, Jesús Rafael Rodríguez Amado, Univ Fed Amapa, Univ Sierra, Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), and Univ Nacl Autonoma Mexico
- Subjects
biology ,In silico ,Bixin ,General Medicine ,Pharmacology ,Hypoglycemia ,medicine.disease ,biology.organism_classification ,PPAR gamma ,chemistry.chemical_compound ,Bixa ,in vivo ,FÁRMACOS IMUNOSSUPRESSORES ,hypoglycemia ,chemistry ,Structural Biology ,In vivo ,in silico ,Alloxan ,medicine ,Molecular Biology - Abstract
Made available in DSpace on 2020-12-10T19:49:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-02-12 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Procad Amazonia Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The objectives of this study were to extract and purify Bixin from the seeds of Bixa orellana and to evaluate its hypoglycemic activity in vivo, as well as, to conduct an in silico study of selectivity on peroxisome proliferator-activated receptors via molecular docking and molecular dynamics simulations. Oral administration of Bixin (10 mg/kg) significantly reduced their glucose level that was alloxan-induced diabetic rats. Bixin showed in silico selectivity on peroxisome proliferator-activated receptors (PPARs), particularly by the peroxisome proliferator-activated receptor gamma (PPAR gamma), which supports the hypoglycemic activity of Bixin. From the results obtained, it can be inferred that Bixin presents hypoglycemic characteristics, which was confirmed by the results obtained from the in vivo and in silico tests. Bixin may act by other pathways to control blood glucose and thus it is possible that it presents a different toxicity profile than troglitazone, rosiglitazone and pioglitazone. However, more studies on the activity and toxicity of Bixin are needed to evaluate for further clinical use. Univ Fed Amapa, Dept Biol Sci & Hlth, Lab Drugs Discovery, Macapa, Brazil Univ Sierra, Div Postgrade, Ixtlan De Juarez, Mexico Univ Fed Amapa, Dept Biol Sci & Hlth, Lab Modeling & Computat Chem, Macapa, Brazil Univ Fed Amapa, Res Grp Biocatalysis & Apllied Organ Synth, Macapa, Brazil Sao Paulo State Univ, Dept Nat Act Principles & Toxicol, Fac Pharmaceut Sci, Araraquara, SP, Brazil Univ Sao Paulo, Dept Cellular & Mol Biol, Ribeirao Preto Med Sch, Ribeirao Preto, Brazil Univ Nacl Autonoma Mexico, Fac Chem, Dept Pharm, Lab Pharmacol Nat Prod, Mexico City, DF, Mexico Univ Sao Paulo, Ribeirao Preto Med Sch, Bioinformat Grp, Dept Genet, Ribeirao Preto, Brazil Sao Paulo State Univ, Dept Nat Act Principles & Toxicol, Fac Pharmaceut Sci, Araraquara, SP, Brazil CAPES: PNPD/20130076-14001012005P1 Procad Amazonia: Auxpe - 1723/2018 CNPq: 407768/2013-0
- Published
- 2020
42. Assessment of the hypoglycemic effect of Bixin in alloxan-induced diabetic rats
- Author
-
Hady, Keita, Cleydson Breno Rodrigues, Dos Santos, Matheus Mercês, Ramos, Elias Carvalho, Padilha, Rodolfo Bortolozo, Serafim, Andres Navarrete, Castro, Jesus Rafael Rodriguez, Amado, Gabriel Monteiro, da Silva, Irlon Maciel, Ferreira, Silvana, Giuliatti, and José Carlos Tavares, Carvalho
- Subjects
Molecular Docking Simulation ,PPAR gamma ,Alloxan ,Animals ,Hypoglycemic Agents ,Thiazolidinediones ,Carotenoids ,Diabetes Mellitus, Experimental ,Rats - Abstract
The objectives of this study were to extract and purify Bixin from the seeds of
- Published
- 2020
43. Chemoenzymatic Synthesis and Anti-Inflammatory Activity of Fatty Acid Amides Prepared from Bertholletia excelsa (Brazil Nut) Triglycerides
- Author
-
Albenise Santana Alves Barros, Edilene O. Silva, Adan Galué-Parra, Helison de Oliveira Carvalho, José Carlos Tavares Carvalho, Pedro H. da S. Barata, Irlon M. Ferreira, Ícaro Rodrigues Sarquis, and Alex Bruno Lobato Rodrigues
- Subjects
chemistry.chemical_classification ,biology ,Chemistry ,medicine.drug_class ,Fatty acid ,Pseudomonas fluorescens ,General Chemistry ,Anandamide ,biology.organism_classification ,food.food ,Anti-inflammatory ,chemistry.chemical_compound ,food ,Edema ,medicine ,biology.protein ,Bertholletia ,Food science ,medicine.symptom ,Lipase ,Brazil nut - Abstract
This study reports the synthesis of fatty acid amides (FAA), analogous to anandamide, prepared from Brazil nut oil (BNO), using Amano lipase from Pseudomonas fluorescens as a catalyst of direct aminolysis, with a good yield (95-68%). The anti-inflammatory effects of BNO and FAA were evaluated by the carrageenan-induced paw edema method. Analysis of the formation of edema showed that these FAA, at all dose used, significantly reduced the development of edema.
- Published
- 2020
44. Leaves of Spondias mombin L. a traditional anxiolytic and antidepressant: Pharmacological evaluation on zebrafish (Danio rerio)
- Author
-
José Luis Balderas, Elizabeth Arlen Pineda-Peña, Hady Keita, Gerley Anatê da Silva Aleluia, Bianca Thais de Freitas Paiva, Fernando Luiz Pinheiro da Silva Neto, Nayara Costa de Melo, Brenda Lorena Sánchez-Ortiz, Heitor Ribeiro da Silva, Rodrigo A.S. Cruz, Andrés Navarrete, Tafnis Ingret dos Santos Sampaio, and José Carlos Tavares Carvalho
- Subjects
Male ,0301 basic medicine ,medicine.drug_class ,Anacardiaceae ,Danio ,Anxiolytic ,Buspirone ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Oral administration ,Drug Discovery ,medicine ,Animals ,Zebrafish ,Spondias mombin ,Pharmacology ,Fluoxetine ,Thigmotaxis ,Behavior, Animal ,biology ,Traditional medicine ,Plant Extracts ,biology.organism_classification ,Antidepressive Agents ,Plant Leaves ,030104 developmental biology ,Anti-Anxiety Agents ,Social Isolation ,chemistry ,Female ,Caffeine ,Stress, Psychological ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Ethnobotanical relevance Spondias mombin L. is a plant dispersed throughout the tropical regions of South America, Africa, and Asia, being found mainly in the North and Northeast of Brazil, where the leaves are used in preparations for neuropsychiatric disorders. Therefore, it is of great importance to carry out studies in different pharmacological models that can prove the traditional use of this plant species. Materials and methods the hydroethanolic extract from S. mombin leaves (HELSm) was evaluated by oral administration (25 mg/kg) and by immersion (25 mg/l) in scototaxis test in zebrafish (Danio rerio). For this study, caffeine (100 mg/kg) and buspirone (25 mg/kg) were used as standard drugs. The antidepressant action of the HELSm was evaluated assessed in the novel tank diving test (NTDT). In this study, a group with 1% ethanol, one with unpredictable chronic mild stress (UCMS), and another with developmental, social isolation (DSI) were used as induction groups for depression-like behavior and fluoxetine (20 mg/kg) as a drug pattern. Results by the HPLC-UV fingerprint analysis, the HELSm presented several derivatives of polyphenolic compounds and flavonoids and identified ellagic acid and isoquercitrin, and by the gas-chromatographic, the majority of the identified compounds were fatty acids, esters, and alcohols. By immersion, the LC50 was 49.86 mg/l and by oral via the LD50 in 48 h, was 4.515 g/kg in zebrafish. For all spatiotemporal and behavioral variables (time spent, white compartment, latency, toggle, erratic swimming, freezing duration, thigmotaxis, and risk assessment), the treatment with HELSm produced a similar effect to buspirone and was significant when compared to the caffeine and control group (p Conclusions Our results suggest that the acute administration of the HELSm in the scototaxis and NTDT tests in a zebrafish model (Danio rerio) produced anxiolytic and antidepressant effects, devoid of hypnotic and sedative actions by immersion, and this action was improved when administered by oral via. Possibly, the presence of isoquercitrin in the leaves of Spondias mombin participates in the anxiolytic and antidepressant effects.
- Published
- 2018
45. Nanosuspension of quercetin: preparation, characterization and effects against Aedes aegypti larvae
- Author
-
Anna Eliza Maciel de Faria Motta Oliveira, Raquel Silva Araújo, Ricardo Marcelo dos Anjos Ferreira, Jonatas Lobato Duarte, Silvia Maria Mathes Faustino, Caio P. Fernandes, Rodrigo A.S. Cruz, Leticie Zulmira da Silva Pessoa, Raimundo Nonato Picanço Souto, and José Carlos Tavares Carvalho
- Subjects
0301 basic medicine ,Larvicide ,Chlorella vulgaris ,Flavonoid ,lcsh:RS1-441 ,02 engineering and technology ,Aedes aegypti ,lcsh:Pharmacy and materia medica ,03 medical and health sciences ,chemistry.chemical_compound ,Zeta potential ,heterocyclic compounds ,Food science ,Ecotoxicity ,General Pharmacology, Toxicology and Pharmaceutics ,chemistry.chemical_classification ,biology ,Chemistry ,fungi ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Solvent ,030104 developmental biology ,Culicidae ,Nanocarrier ,0210 nano-technology ,Quercetin - Abstract
Aedes aegypti (Diptera: Culicidae) is the main vector of some neglected diseases, including dengue. It is very important to develop formulations that increase effectiveness of vector control with low toxicity. Quercetin is a plant-derived flavonoid that modulates the development of some insects. The low water solubility of quercetin impairs the development of water-dispersible commercial products. To circumvent this problem, the preparation of nanoformulations is considered promising. Thus, this study aimed to evaluate the effect of bulk and quercetin nanosuspension against A. aegypti larvae and also to investigate their ecotoxicity. Quercetin nanosuspension was produced by a solvent displacement method followed by solvent evaporation and was maintained in two different temperatures (4 and 25 °C). Its size distribution and zeta potential were monitored along 30 days. The influence of quercetin nanosuspension and bulk-quercetin was investigated at various concentrations against A. aegypti and the green algae Chlorella vulgaris. The quercetin nanosuspension presented higher stability at 4 °C and negative zeta potential values. Quercetin nanosuspension and bulk-quercetin adversely affected the larvae development, especially at the highest concentrations. Larvae mortality was between 44% and 100% (48 h) for quercetin nanosuspension at 100 and 500 ppm, respectively. The bulk-quercetin induced around 50% mortality regardless the concentration used at this same time-period. Absence of emerging mosquitoes from water was observed on the survival larvae of all the treated groups. Quercetin nanosuspension was less toxic than bulk-quercetin against C. vulgaris, especially at higher concentrations. These data indicate that quercetin nanosuspension may represent a potential larvicide for A. aegypti control, once they induced larvae death and inhibited the survival ones to emerge from water. In addition, it did not demonstrated ecotoxicity against a non-target organism, highlighting its better properties, when compared to the bulk-quercetin. Keywords: Culicidae, Ecotoxicity, Larvicide, Nanocarrier, Flavonoid
- Published
- 2018
46. Acute Toxicity of the Hydroethanolic Extract of the Flowers of Acmella oleracea L. in Zebrafish (Danio rerio): Behavioral and Histopathological Studies
- Author
-
José Carlos Tavares Carvalho, Wagner Luiz Ramos Barbosa, Muller Duarte Viana, Gisele Custódio de Souza, Nayara Costa de Melo, Irlon M. Ferreira, Monaliza Maia Rebelo de Oliveira, Brenda Lorena Sánchez-Ortiz, and Ianna Dias Ribeiro da Silva
- Subjects
Pharmaceutical Science ,Biology ,Pharmacology ,Median lethal dose ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Oral administration ,Drug Discovery ,medicine ,Acmella oleracea ,030304 developmental biology ,0303 health sciences ,Kidney ,oral and immersion ,Danio rerio ,toxicity ,Spilanthol ,biology.organism_classification ,Acute toxicity ,medicine.anatomical_structure ,chemistry ,Phytochemical ,030220 oncology & carcinogenesis ,Toxicity ,histopathology ,Molecular Medicine - Abstract
Hydroethanolic preparations of the botanical species Acmella oleracea L. are used in the north of Brazil for the treatment of various diseases. However, few studies have been conducted to evaluate the toxicity of this species. The objective of this study was to evaluate the acute toxicity of the hydroethanolic extract of A. oleracea L. (EHFAo) flowers in zebrafish by immersion and oral administration. The extract was analyzed by ultra-performance liquid chromatography&ndash, mass spectrometry (UPLC&ndash, MS). EHFAo was administered orally (44.457, 88.915, 199.94, 281.83, and 448.81 mg/kg) and by immersion (250, 300, 350, 400, and 450 µ, g/L). Behavioral and histopathological analysis of gills, liver, intestine, and kidney were performed. The presence of (2E,6Z,8E)-N-isobutyl-2,6,8-decatrienamide (spilanthol) in EHFAo was identified by ultra-high-re.solution liquid chromatography&ndash, electrospray ionization mass spectrometry (UHPLC&ndash, ESI-MS). Treatment with EHFAo caused significant behavioral changes and death. The calculated median lethal dose (LD50) was 148.42 mg/kg, and the calculated median lethal concentration (LC50) was 320 &mu, g/L. In the histopathological study, it was observed that upon oral treatment, the tissue alterations that compromised the normal functioning of the organism occurred with EHFAo doses of 88.915, 199.53, and 281.83 mg/kg, the intestine being the most affected. When the treatment was performed by immersion, the most toxic EHFAo concentrations according to the histopathological evaluation were 300, 350, and 400 &mu, g/L, with the most affected organ being the gills. Finally, EHFAo in this study was shown to be more toxic to the liver, intestine, and kidneys when administered orally and to gills, liver, and kidneys when administered by immersion in water. Therefore, considering the results obtained and the chemical characteristics of the main phytochemical marker of EHFAo, spilanthol, it can be suggested that, depending on the dose, this compound can lead to histopathological damages in the organs highlighted in this study.
- Published
- 2019
- Full Text
- View/download PDF
47. Libidibia ferrea (jucá), a Traditional Anti-Inflammatory: A Study of Acute Toxicity in Adult and Embryos Zebrafish (Danio rerio)
- Author
-
Diego Quaresma Ferreira, José Carlos Tavares Carvalho, Rodrigo Alves Souza Cruz, Brenda Lorena Sánchez Ortiz, Jemima C. M. M. Miranda, Gisele Custódio de Souza, Rosangela S. F. R. Sarquis, Raquel Silva Araújo, Rafael Garrett, Caio P. Fernandes, Anna E.M.F.M. Oliveira, and Thamara de Oliveira Ferraz
- Subjects
0301 basic medicine ,medicine.drug_class ,Danio ,Pharmaceutical Science ,Anti-inflammatory ,Article ,03 medical and health sciences ,Drug Discovery ,medicine ,Caesalpinia ,Zebrafish ,biology ,Traditional medicine ,0402 animal and dairy science ,Libidibia ferrea ,toxicity ,Embryo ,04 agricultural and veterinary sciences ,biology.organism_classification ,zebrafish ,040201 dairy & animal science ,Acute toxicity ,030104 developmental biology ,Toxicity ,histopathology ,Molecular Medicine ,embryos - Abstract
The plant species Libidibia ferrea (Mart. ex Tul.) LP Queiroz var. ferrea basionym of Caesalpinia ferrea (Mart. ex Tul.) is used in various regions of Brazil in folk medicine in the treatment of several health problems, especially in acute and chronic inflammatory processes. Most of the preparations employed are alcoholic. Therefore, this study aimed to evaluate the acute toxicity of the hydroethanolic extract of fruits of Libidibia ferrea (EHEFLf) in zebrafish, emphasizing the possible changes in the organic-cellular level of the gills, liver, kidneys, and intestine and on embryos. The result obtained by LC-M/MS from EHEFLf indicated a high concentration of possible polyhydroxylated substances. EHEFLf, at a dose of 2 g/kg orally, produced non-significant alterations of the analyzed organs. However, for embryos, the treatment with different concentrations demonstrated heart toxicity that was concentration-dependent. There is no evidence of a correlation of the observed effects with the phytochemical composition, and considering the species of animal used, it can be suggested that the oral use of L. ferrea hydroethanolic extract has an acceptable degree of safety for use as an oral medicinal product. and embryo results have shown significant affinity to the heart, however, it is perceived to be related to the concentrations used.
- Published
- 2019
48. Larvicidal effect of hydroethanolic extract from the leaves of Acmella oleracea L. R. K. Jansen in Aedes aegypti and Culex quinquefasciatus
- Author
-
Irlon M. Ferreira, A. L. Lima, I.F. de Araújo, Ricardo Marcelo dos Anjos Ferreira, Raimundo Nonato Picanço Souto, José Carlos Tavares Carvalho, Iracirema da Silva Sena, and P.H.F. de Araújo
- Subjects
0106 biological sciences ,0301 basic medicine ,animal structures ,biology ,Traditional medicine ,fungi ,virus diseases ,Plant Science ,Aedes aegypti ,biology.organism_classification ,medicine.disease ,01 natural sciences ,Culex quinquefasciatus ,Dengue fever ,Zika virus ,Filariasis ,03 medical and health sciences ,030104 developmental biology ,Vector (epidemiology) ,parasitic diseases ,medicine ,Medicinal plants ,Acmella oleracea ,010606 plant biology & botany - Abstract
Mosquitoes, such as Aedes aegypti and Culex quinquefasciatus, are important vectors of diseases, such as dengue fever, chikungunya fever, Zika virus, and filariasis, and these diseases are public health problems. The present study was carried out to evaluate the larvicidal activity of the hydroethanolic extract from leaves of Acmella oleracea leaves against 3rd instar larvae of the Ae. aegypti dengue vector and the Cx. quinquefasciatus filariasis vector. The hydroethanolic extract caused significant mortality in Ae. aegypti and Cx. quinquefasciatus. After 24 h of exposure to the extract, it was possible to establish the LC50 values for the extract: 11.41 ppm for Ae. aegypti and LC50 32.40 ppm for Cx. quinquefasciatus. The hydroethanolic extract from leaves of A. oleracea showed very low ecotoxicity suggesting that it can be used without causing environmental damage. This is the first study that shows the use of hydroethanolic extract from leaves of A. oleracea as an alternative to synthetic larvicides to eliminate larvae of Ae. aegypti and Cx. quinquefasciatus in an easy, cheap and safe way.
- Published
- 2018
49. Direct and Solvent-Free Aminolysis of Triglyceride from Oenocarpus bataua (Patawa) Oil Catalyzed by Al2O3
- Author
-
José Carlos Tavares Carvalho, Didier Bereau, Raquel R. Amaral, Inana F. Araújo, Pedro H. da S. Barata, Irlon M. Ferreira, Pedro H. F. de Araújo, and Jhone M. Curti
- Subjects
chemistry.chemical_classification ,biology ,Triglyceride ,010405 organic chemistry ,Chemistry ,Fatty acid ,General Chemistry ,010402 general chemistry ,biology.organism_classification ,Antimicrobial ,Heterogeneous catalysis ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Solvent ,chemistry.chemical_compound ,Aminolysis ,Oenocarpus bataua ,Organic chemistry - Abstract
The amidation of fatty acid is an important reaction for synthesis of fatty alkolamines with a variety of pharmacological activities. The objective of this paper was to demonstrate the aminolysis direct and solvent of the triglycerides from the patawa oil by heterogeneous catalysis. The results of the amidation direct free solvents were favorable using 5% Al2O3 as catalyst, with a conversion of 94% at 24 h of reaction. After spectroscopic characterization, we decided explore the antimicrobial tests of the fatty amides (FAA) against the Staphylococcus aureus strain (ATCC 33591), showing a MIC activity of 1.9612 mg/mL and the fatty ethyl esters (FAEE) a 3.4125 mg/mL MIC. Therefore, the synthesis direct free solvents of FAA by Al2O3 from patawa oil was viable and innovative.
- Published
- 2018
50. Secondary Metabolites Found among the Species Trattinnickia rhoifolia Willd
- Author
-
Agerdânio Andrade de Souza, Brenda Lorena Sánchez Ortíz, Rosemary de Carvalho Rocha Koga, Priscila Faimann Sales, Divino Bruno da Cunha, Ana Luiza Mantovaneli Guerra, Gisele Custódio de Souza, and José Carlos Tavares Carvalho
- Subjects
Plant Extracts ,Phytochemicals ,Trattinnickia ,Organic Chemistry ,review ,Pharmaceutical Science ,Antioxidants ,Analytical Chemistry ,QD241-441 ,Anti-Infective Agents ,Chemistry (miscellaneous) ,Drug Discovery ,Humans ,phytochemistry ,Molecular Medicine ,pharmacology ,Physical and Theoretical Chemistry ,Burseraceae ,Brazil ,Anesthetics - Abstract
Plant-derived products may represent promising strategies in the treatment of Neglected Tropical Diseases (NTDs). From this perspective, it is observed that the Amazon phytogeographic region contains the tribe Canarieae of the Burseraceae family, composed of trees and shrubs supplied with resin channels. Its uses in folk medicine are related to aromatic properties, which have numerous medicinal applications and are present in reports from traditional peoples, sometimes as the only therapeutic resource. Despite its economic and pharmacological importance in the region, and although the family is distributed in all tropical and subtropical regions of the world, most of the scientific information available is limited to Asian and African species. Therefore, the present work aimed to review the secondary metabolites with possible pharmacological potential of the species Trattinnickia rhoifolia Willd, popularly known as “Breu sucuruba”. To this end, an identification key was created for chemical compounds with greater occurrence in the literature of the genus Trattinnickia. The most evident therapeutic activities in the consulted studies were antimicrobial, antioxidant, anti-inflammatory, antiviral, antifungal, anesthetic and antiparasitic. An expressive chemical and pharmacological relevance of the species was identified, although its potential is insufficiently explored, mainly in the face of the NTDs present in the Brazilian Amazon.
- Published
- 2021
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.