37 results on '"Jooyoung Jung"'
Search Results
2. 'Assessing University Faculty’s Perceptions and Needs on Developing Teaching Competencies: An IPA Approach'
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Jooyoung Jung and Suyoun Byoun
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- 2022
3. Analysis of the Relationship Between Self-management and Interpersonal Relationship Competency, Academic Stress, Academic Continuity in University
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Jooyoung Jung
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General Medicine - Published
- 2022
4. A Study on the Improvement of Learning Outcome of University Students: Focusing on majoring in Social Welfare
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Yujin Im and JooYoung Jung
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Medical education ,Social Welfare ,Psychology ,Outcome (game theory) - Published
- 2020
5. Phenomenological Study on Motherhood Experiences of Female Inmates
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JooYoung Jung
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Psychology ,Developmental psychology - Published
- 2019
6. ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain
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Uhtaek Oh, H. J. Kim, Yongwoo Jang, Sung Hoon Lee, Soo Jin Oh, Gyu Sang Hong, Jae Hyouk Choi, In-Beom Kim, Jooyoung Jung, Eun Mi Hwang, and Byeongjun Lee
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Down-Regulation ,Anoctamin 1 ,ANO1 ,Mice ,neural stem cell ,Chlorides ,Neurotrophic factors ,medicine ,Animals ,cortical development ,Progenitor cell ,Anoctamin-1 ,Mice, Knockout ,TMEM16A ,Gene knockdown ,Multidisciplinary ,biology ,Brain-Derived Neurotrophic Factor ,Brain ,Biological Sciences ,Embryonic stem cell ,eye diseases ,Radial glial cell ,Neural stem cell ,Up-Regulation ,Cell biology ,Mice, Inbred C57BL ,medicine.anatomical_structure ,biology.protein ,Chloride channel ,sense organs ,Neuroglia ,Neuroscience ,radial glial cell - Abstract
Significance Radial glial cells (RGCs), a type of neural stem cell in the developing brain, not only generate progenitors, newly born neurons and glial cells, but also deliver neurons through its process to the appropriate cortical target layers. Thus, the function of RGCs is crucial for cortex development, in which Cl− channels are thought to play a role. Here we highlight that Anoctamin 1 (ANO1)/TMEM16A, a Ca2+-activated Cl− channel, mediates the process extension in RGCs. ANO1-null mice show a decrease in cortical thickness with disorganized cortical layers. Thus, as a Cl− channel, ANO1 is involved in the process maturation of RGCs and contributes to cortex development., Neural stem cells (NSCs) are primary progenitor cells in the early developmental stage in the brain that initiate a diverse lineage of differentiated neurons and glia. Radial glial cells (RGCs), a type of neural stem cell in the ventricular zone, are essential for nurturing and delivering new immature neurons to the appropriate cortical target layers. Here we report that Anoctamin 1 (ANO1)/TMEM16A, a Ca2+-activated chloride channel, mediates the Ca2+-dependent process extension of RGCs. ANO1 is highly expressed and functionally active in RGCs of the mouse embryonic ventricular zone. Knockdown of ANO1 suppresses RGC process extension and protrusions, whereas ANO1 overexpression stimulates process extension. Among various trophic factors, brain-derived neurotrophic factor (BDNF) activates ANO1, which is required for BDNF-induced process extension in RGCs. More importantly, Ano1-deficient mice exhibited disrupted cortical layers and reduced cortical thickness. We thus conclude that the regulation of RGC process extension by ANO1 contributes to the normal formation of mouse embryonic brain.
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- 2019
7. Exploring the Effects of Contextual Factors on Home Lighting Experience
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Kwangmin Cho, Chajoong Kim, Soyoung Kim, and Jooyoung Jung
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0301 basic medicine ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Visual Arts and Performing Arts ,Architecture ,Computer Graphics and Computer-Aided Design ,030217 neurology & neurosurgery - Published
- 2018
8. Chloride Channels in Nociceptors
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Uhtaek Oh and Jooyoung Jung
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Bestrophin 1 ,Nociception ,nervous system ,biology ,Chemistry ,GABAA receptor ,Nociceptor ,biology.protein ,Biophysics ,Chloride channel ,Anoctamin-1 - Abstract
Pain may be induced by activation of various ion channels expressed in primary afferent neurons. These channels function as molecular sensors that detect noxious chemical, temperature, or tactile stimuli and transduce them into nociceptor electrical signals. Transient receptor potential channels are good examples because they are activated by chemicals, heat, cold, and acid in nociceptors. Anion channels were little studied in nociception because of the notion that anion channels might induce hyperpolarization of nociceptors on opening. In contrast, opening of Cl- channels in dorsal root ganglion (DRG) neurons depolarizes sensory neurons, resulting in excitation of nociceptors, thereby inducing pain. Anoctamin 1(ANO1)/TMEM16A is a Ca2+-activated Cl- channel expressed mainly in small DRG neurons, suggesting a nociception role. ANO1 is a heat sensor that detects heat over 44°C. Ano1-deficient mice elicit less nocifensive behaviors to hot temperatures. In addition, mechanical allodynia and hyperalgesia induced by inflammation or nerve injury are alleviated in Ano1-/- mice. More important, Ano1 transcripts are increased in chronic pain models. Bestrophin 1 (Best1) is another Ca2+-activated Cl- channel expressed in nociceptors. Best1 is increased in axotomized DRG neurons. The role of Best1 in nociception is not clear. GABAA receptors are in the central process of DRG neurons; GABA depolarizes the primary afferents. This depolarization consists of primary afferent depolarization essential for inhibiting nociceptive input to second-order neurons in the spinal cord, regulating pain signals to the brain. Thus, although Cl- channels in nociceptors are not as numerous as TRP channels, their role in nociception is distinct and significant.
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- 2019
9. Tentonin 3/TMEM150c Confers Distinct Mechanosensitive Currents in Dorsal-Root Ganglion Neurons with Proprioceptive Function
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Jungwon Wee, Gyu-Sang Hong, Uhtaek Oh, Jooyoung Jung, Byeongjun Lee, In-Beom Kim, Joo Young Cha, Tae-Ryong Riew, Hyeyeon Chun, H. J. Kim, and Gyu Hyun Kim
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0301 basic medicine ,Muscle spindle ,Mice, Transgenic ,Biology ,Ion Channels ,03 medical and health sciences ,0302 clinical medicine ,Dorsal root ganglion ,Ganglia, Spinal ,medicine ,Animals ,Cells, Cultured ,Ion channel ,Neurons ,Proprioception ,General Neuroscience ,PIEZO1 ,Membrane Proteins ,Motor coordination ,030104 developmental biology ,medicine.anatomical_structure ,Touch ,Biophysics ,Mechanosensitive channels ,Ion Channel Gating ,Mechanoreceptors ,Transduction (physiology) ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Touch sensation or proprioception requires the transduction of mechanical stimuli into electrical signals by mechanoreceptors in the periphery. These mechanoreceptors are equipped with various transducer channels. Although Piezo1 and 2 are mechanically activated (MA) channels with rapid inactivation, MA molecules with other inactivation kinetics have not been identified. Here we report that heterologously expressed Tentonin3 (TTN3)/TMEM150C is activated by mechanical stimuli with distinctly slow inactivation kinetics. Genetic ablation of Ttn3/Tmem150c markedly reduced slowly adapting neurons in dorsal-root ganglion neurons. The MA TTN3 currents were inhibited by known blockers of mechanosensitive ion channels. Moreover, TTN3 was localized in muscle spindle afferents. Ttn3-deficient mice exhibited the loss of coordinated movements and abnormal gait. Thus, TTN3 appears to be a component of a mechanosensitive channel with a slow inactivation rate and contributes to motor coordination. Identification of this gene advances our understanding of the various types of mechanosensations, including proprioception.
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- 2016
10. Investigation of Electromagnetic Force for Magnetic Contactor of Railway Vehicles
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Jooyoung Jung, Jinnil Choi, and Ji-Won Park
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010302 applied physics ,Engineering ,business.industry ,Strategy and Management ,Geography, Planning and Development ,Electrical engineering ,Energy Engineering and Power Technology ,Mechanical engineering ,Transportation ,01 natural sciences ,010305 fluids & plasmas ,0103 physical sciences ,Automotive Engineering ,business ,Civil and Structural Engineering ,Contactor - Published
- 2016
11. Effects of Grid Characteristics on High Speed Circuit Breaker for Railway Vehicle
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Jooyoung Jung, Jinnil Choi, and Ji-Won Park
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010302 applied physics ,Engineering ,business.industry ,Strategy and Management ,020208 electrical & electronic engineering ,Geography, Planning and Development ,Electrical engineering ,Energy Engineering and Power Technology ,Transportation ,02 engineering and technology ,Grid ,01 natural sciences ,0103 physical sciences ,Automotive Engineering ,0202 electrical engineering, electronic engineering, information engineering ,business ,Circuit breaker ,Civil and Structural Engineering - Published
- 2016
12. Anoctamin 9/TMEM16J is a Cation Channel Activated by cAMP/PKA Signal
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Hyesu Kim, H. J. Kim, Jooyoung Jung, Jesun Lee, Mi-Ock Lee, Uhtaek Oh, Hee-Ryang Kim, and Byeongjun Lee
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0301 basic medicine ,Phospholipid scramblase ,Physiology ,Anoctamins ,Intracellular Space ,medicine.disease_cause ,Calcium in biology ,ANO1 ,Cell membrane ,03 medical and health sciences ,Cyclic AMP ,medicine ,Animals ,Humans ,Phospholipid Transfer Proteins ,Phosphorylation ,Protein kinase A ,Molecular Biology ,biology ,Chemistry ,Sodium ,Cholera toxin ,Membrane Proteins ,Cell Biology ,Cyclic AMP-Dependent Protein Kinases ,Cell biology ,Intestines ,Mice, Inbred C57BL ,HEK293 Cells ,030104 developmental biology ,medicine.anatomical_structure ,Membrane protein ,Biochemistry ,biology.protein ,Biophysics ,Calcium ,Ion Channel Gating ,Intracellular ,Signal Transduction - Abstract
Anoctamins (ANOs) are multifunctional membrane proteins that consist of 10 homologs. ANO1 (TMEM16A) and ANO2 (TMEM16B) are anion channels activated by intracellular calcium that meditate numerous physiological functions. ANO6 is a scramblase that redistributes phospholipids across the cell membrane. The other homologs are not well characterized. We found ANO9/TMEM16J is a cation channel activated by a cAMP-dependent protein kinase A (PKA). Intracellular cAMP-activated robust currents in whole cells expressing ANO9, which were inhibited by a PKA blocker. A cholera toxin that persistently stimulated adenylate cyclase activated ANO9 as did the application of PKA. The cAMP-induced ANO9 currents were permeable to cations. The cAMP-dependent ANO9 currents were augmented by intracellular Ca2+. Ano9 transcripts were predominant in the intestines. Human intestinal SW480 cells expressed high levels of Ano9 transcripts and showed PKA inhibitor-reversible cAMP-dependent currents. We conclude that ANO9 is a cation channel activated by a cAMP/PKA pathway and could play a role in intestine function.
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- 2017
- Full Text
- View/download PDF
13. Voluntary Movements as a Possible Non-Reflexive Pain Assay
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Hawon Cho, Uhtaek Oh, Byeongjun Lee, Hyeyoun Chun, Sun Wook Hwang, Jooyoung Jung, Yongwoo Jang, and Sung Min Kim
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Male ,medicine.medical_specialty ,Neurology ,Rearing ,Inflammatory pain ,Total distance moved ,Pain medicine ,Voluntary movement ,Pain ,TRPV Cation Channels ,Mice, Inbred Strains ,Motor Activity ,Neuropathic pain ,Carrageenan ,Mice ,Cellular and Molecular Neuroscience ,Pain assessment ,medicine ,Animals ,Pain Measurement ,Inflammation ,Analgesics ,Hypoalgesia ,business.industry ,Research ,Anti-Inflammatory Agents, Non-Steroidal ,Visceral pain ,TRPV1 ,medicine.disease ,Rats ,Disease Models, Animal ,Anesthesiology and Pain Medicine ,Anesthesia ,Morphine ,Neuralgia ,Molecular Medicine ,medicine.symptom ,business ,medicine.drug - Abstract
Background The quantification of pain intensity in vivo is essential for identifying the mechanisms of various types of pain or for evaluating the effects of different analgesics. A variety of behavioral tests for pain measurement have been devised, but many are limited because animals are physically restricted, which affects pain sensation. In this study, pain assessment was attempted with minimal physical restriction, and voluntary movements of unrestrained animals were used to evaluate the intensities of various types of pain. Results The number of times animals reared or total distances traveled was measured using a motion-tracking device and found to be markedly reduced in carrageenan-induced inflammatory, acetic acid-induced visceral, and streptozotocin-induced neuropathic pain tests. These two voluntary movement parameters were found to be highly correlated with paw withdrawal latency from irradiating heat. In addition, these parameters were markedly reversed by morphine and by non-steroidal anti-inflammatory drugs in inflammatory pain models. These parameters were also useful to detect hypoalgesia in TRPV1−/− mice. Conclusions These results suggest that parameters of voluntary movement, such as, number of rearing and total distance moved, are effective indicators of pain intensity for many types of pain and that they can be used to evaluate degree of pain perception.
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- 2013
14. Quantitative analysis of TRP channel genes in mouse organs
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Uhtaek Oh, Young Duk Yang, Jooyoung Jung, Yongwoo Jang, Yunjong Lee, and Sung Min Kim
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Male ,medicine.medical_specialty ,TRPV5 ,TRPV2 ,Gene Expression ,Biology ,Real-Time Polymerase Chain Reaction ,TRPC1 ,Mice ,Transient receptor potential channel ,Transient Receptor Potential Channels ,TRPC3 ,TRPM ,Internal medicine ,Drug Discovery ,medicine ,Animals ,TRPM3 ,RNA, Messenger ,TRPM5 ,Mice, Inbred ICR ,Gene Expression Profiling ,Organic Chemistry ,Cell biology ,Endocrinology ,Organ Specificity ,Molecular Medicine - Abstract
The transient receptor potential (TRP) channel superfamily is a set of channel genes that mediate numerous physiological functions such as sensing irritants or detecting temperature changes. Despite their functions, expressional information on TRP channels in various organs is largely elusive. Therefore, we conducted a systematic quantitative comparison of each mRNA expression level of 22 mouse TRP channels in various organs. As a result, we found that average levels of TRP channel transcripts were very low reaching ∼3% of the GAPDH transcript level. Among 22 TRP channels, TRPC1 and TRPM7 were most abundant in the majority of organs. In contrast, TRPV3, TRPV5, TRPV6, TRPC7, TRPM1, and TRPM5 elicited very low message profiles throughout the major organs. Consistent with their functions as molecular sensors for irritants and temperature changes, TRPV1, TRPM8 and TRPA1 showed exclusive expression in sensory ganglia. TRPC3 and TRPM3 were abundant in the sensory ganglia and brain. High levels of transcripts of TRPV2, TRPC6, TRPM4, and TRPM6 were observed in the lung. In addition, channel transcript levels were very low except TRPM7 in the liver. In summary, the expression profile of TRP channels in major tissues provides insight to their physiological functions and therefore application to new drug development.
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- 2012
15. Axonal Neuropathy-associated TRPV4 Regulates Neurotrophic Factor-derived Axonal Growth
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Yongwoo Jang, Dong-Jin Yang, Hawon Cho, Uhtaek Oh, Ji Hyun Jeon, Saewoon Jung, Jungeun Oh, In-Beom Kim, H. J. Kim, Kyung-Tai Kim, Jooyoung Jung, Mi-Ryoung Song, and Sung Min Kim
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TRPV4 ,Neurite ,TRPV Cation Channels ,Cell Growth Processes ,Biology ,PC12 Cells ,Biochemistry ,Mice ,Neurotrophic factors ,Phorbol Esters ,Cell Adhesion ,Cyclic AMP ,Neurites ,medicine ,Animals ,Humans ,Nerve Growth Factors ,Peripheral Nerves ,RNA, Messenger ,Protein Structure, Quaternary ,Molecular Biology ,Arachidonic Acid ,Neurodegeneration ,Cell Biology ,Spinal muscular atrophy ,medicine.disease ,Actins ,Axons ,Rats ,Phospholipases A2 ,Nerve growth factor ,Peripheral neuropathy ,Gene Expression Regulation ,Gene Knockdown Techniques ,biology.protein ,Protein Multimerization ,Hereditary Sensory and Motor Neuropathy ,Neuroscience ,Signal Transduction ,Neurotrophin - Abstract
Spinal muscular atrophy and hereditary motor and sensory neuropathies are characterized by muscle weakness and atrophy caused by the degenerations of peripheral motor and sensory nerves. Recent advances in genetics have resulted in the identification of missense mutations in TRPV4 in patients with these hereditary neuropathies. Neurodegeneration caused by Ca(2+) overload due to the gain-of-function mutation of TRPV4 was suggested as the molecular mechanism for the neuropathies. Despite the importance of TRPV4 mutations in causing neuropathies, the precise role of TRPV4 in the sensory/motor neurons is unknown. Here, we report that TRPV4 mediates neurotrophic factor-derived neuritogenesis in developing peripheral neurons. TRPV4 was found to be highly expressed in sensory and spinal motor neurons in early development as well as in the adult, and the overexpression or chemical activation of TRPV4 was found to promote neuritogenesis in sensory neurons as well as PC12 cells, whereas its knockdown and pharmacologic inhibition had the opposite effect. More importantly, nerve growth factor or cAMP treatment up-regulated the expression of phospholipase A(2) and TRPV4. Neurotrophic factor-derived neuritogenesis appears to be regulated by the phospholipase A(2)-mediated TRPV4 pathway. These findings show that TRPV4 mediates neurotrophic factor-induced neuritogenesis in developing peripheral nerves. Because neurotrophic factors are essential for the maintenance of peripheral nerves, these findings suggest that aberrant TRPV4 activity may lead to some types of pathology of sensory and motor nerves.
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- 2012
16. Anoctamin 1 (TMEM16A) is essential for testosterone-induced prostate hyperplasia
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Mi-Ock Lee, Uhtaek Oh, Beom Chul Chang, Gyu-Sang Hong, Byeongjun Lee, Joo Young Cha, Jungwon Wee, Yongwoo Jang, Jooyoung Jung, Yoon-La Choi, Young Kee Shin, Ho-Young Lee, Tae-Young Na, and Hye-Young Min
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Male ,Small interfering RNA ,Prostatic Hyperplasia ,urologic and male genital diseases ,Pathogenesis ,Prostate ,Genes, Reporter ,Testosterone ,RNA, Small Interfering ,Luciferases ,Promoter Regions, Genetic ,Multidisciplinary ,biology ,Dihydrotestosterone ,Hyperplasia ,Biological Sciences ,Up-Regulation ,Neoplasm Proteins ,medicine.anatomical_structure ,Gene Knockdown Techniques ,Ion Channel Gating ,medicine.drug ,medicine.medical_specialty ,Chromatin Immunoprecipitation ,Response Elements ,Injections ,ANO1 ,Lower urinary tract symptoms ,Chloride Channels ,Internal medicine ,Commentaries ,medicine ,Animals ,Humans ,Rats, Wistar ,Anoctamin-1 ,Cell Proliferation ,urogenital system ,Epithelial Cells ,medicine.disease ,Disease Models, Animal ,Endocrinology ,biology.protein ,Calcium ,Calcium Channels ,Tannins - Abstract
Significance Benign prostatic hyperplasia (BPH) is characterized by an enlargement of the prostate gland, a common disease in elderly men. Excessive testosterone is considered to cause BPH. However, its etiologic mechanisms are elusive. We found that ANO1, a Ca 2+ -activated Cl − channel, is essential for the testosterone-induced BPH. ANO1 was highly expressed in dihydrotestosterone (DHT)-treated prostate epithelial cells. The selective knockdown of ANO1 suppressed DHT-induced cell proliferation. Surprisingly, we found that there were three androgen-response elements in the ANO1 promoter region, which were relevant for the DHT-dependent induction of ANO1. Intraprostate treatment of Ano1 siRNA inhibited the prostate enlargement in vivo. Thus, ANO1 appears essential for the development of prostate hyperplasia and becomes a useful target for treating BPH.
- Published
- 2015
17. Comparison of Intrathecal Chemotherapy for Leptomeningeal Carcinomatosis of a Solid Tumor: Methotrexate Alone Versus Methotrexate in Combination with Cytosine Arabinoside and Hydrocortisone
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Dae-Young Kim, Tae-You Kim, Yung-Jue Bang, Noe Kyeong Kim, Dong Wan Kim, Tak Yun, Keun Wook Lee, Dae Seog Heo, Jooyoung Jung, and Sook Ryun Park
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Adult ,Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Adolescent ,Hydrocortisone ,Adenocarcinoma ,Gastroenterology ,Drug Administration Schedule ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,Meningeal Neoplasms ,medicine ,Carcinoma ,Humans ,Radiology, Nuclear Medicine and imaging ,Carcinoma, Small Cell ,Survival rate ,Injections, Spinal ,Aged ,Retrospective Studies ,business.industry ,Stomach ,Cytarabine ,General Medicine ,Middle Aged ,medicine.disease ,Chemotherapy regimen ,Survival Rate ,Methotrexate ,medicine.anatomical_structure ,Female ,business ,medicine.drug - Abstract
To compare the efficacy of intrathecal methotrexate single therapy with three-drug combination therapy in patients with leptomeningeal carcinomatosis.Fifty-five patients who had pathologically proven leptomeningeal carcinomatosis of a solid tumor were evaluated in terms of pathological response. Group M (n = 29) received methotrexate 15 mg and group MHA (n = 26) received methotrexate 15 mg, hydrocortisone 15 mg/m(2) and ara-C 30 mg/m(2) twice a week intrathecally until a cytological response was obtained.Primary sites of the tumor were the lung (n = 33), breast (n = 13) and stomach (n = 5). The pathology of 45 patients was adenocarcinoma. The cytological response rate to intrathecal chemotherapy was significantly higher in the MHA group than in the M group (38.5 vs 13.8%, P = 0.036). The median survival was 18.6 weeks in the MHA arm and 10.4 weeks in the M arm (P = 0.029).Combination intrathecal chemotherapy with methotrexate, cytosine arabinoside and hydrocortisone showed more favorable effects than methotrexate single therapy for leptomeningeal carcinomatosis in solid tumors.
- Published
- 2003
18. Airway Obstruction after Acute Ozone Exposure in BALB/c Mice Using Barometric Plethysmography
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Chung Ho Yeum, An Soo Jang, Jooyoung Jung, Sun Wook Kim, Byung-Cheol Song, and Inseon S. Choi
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Ozone ,Risk Assessment ,Sensitivity and Specificity ,Statistics, Nonparametric ,Time ,BALB/c ,Mice ,chemistry.chemical_compound ,Reference Values ,Animals ,Medicine ,Plethysmograph ,Ozone exposure ,Plethysmography, Whole Body ,Probability ,Mice, Inbred BALB C ,Enhanced Pause ,biology ,Inhalation ,business.industry ,Sulfuric Acids ,respiratory system ,Airway obstruction ,biology.organism_classification ,medicine.disease ,Respiratory Function Tests ,respiratory tract diseases ,Airway Obstruction ,Disease Models, Animal ,Animals, Newborn ,chemistry ,Anesthesia ,Female ,Original Article ,business ,Bronchoalveolar Lavage Fluid ,Airway responsiveness - Abstract
Background Airway responsiveness after acute inhalation of ozone is related to the concentration and duration of ozone exposure. Using barometric whole-body plethysmography and increase in enhanced pause (Penh) as an index of airway obstruction, we measured the response of BALB/c mice to acute ozone inhalation to study the time course change of pulmonary function after ozone exposure. Methods Penh was measured before and after exposure to filtered air or 0.12, 0.5, 1, or 2 ppm ozone for 3 hr (n=6/group). In addition, Penh was measured 24, 48 and 72 hr after ozone exposure. Bronchoalveolar lavage (BAL) and histopathologic examinations were performed. Results The increase in Penh after ozone exposure was significantly higher in the 0.12, 0.5, 1 and 2 ppm groups compared with the control group (all p
- Published
- 2003
19. Two helices in the third intracellular loop determine anoctamin 1 (TMEM16A) activation by calcium
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Jesun Lee, Min Ho Tak, Young Duk Yang, Soonsil Hyun, Yongwoo Jang, Hawon Cho, Jaehoon Yu, Uhtaek Oh, Byeongjoon Lee, Jungwon Wee, Byung Woo Han, Dong-Jin Yang, Jooyoung Jung, H. Criss Hartzell, Sang Ho Park, and Hyeyeon Chun
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Models, Molecular ,Conformational change ,Physiology ,Surface Properties ,Clinical Biochemistry ,Activation ,Gating ,Plasma protein binding ,Transfection ,Anoctamin 2 ,Anoctamin 1 ,Protein Structure, Secondary ,ANO1 ,Structure-Activity Relationship ,Chloride Channels ,Physiology (medical) ,Humans ,Binding site ,Anoctamin-1 ,Helix ,Binding Sites ,biology ,Chemistry ,Structure ,Surface Plasmon Resonance ,Protein Structure, Tertiary ,Crystallography ,HEK293 Cells ,Mutation ,Chloride channel ,biology.protein ,Biophysics ,Mutagenesis, Site-Directed ,Calcium ,Ion Channel Gating ,Intracellular ,Ion Channels, Receptors and Transporters ,Protein Binding - Abstract
Anoctamin 1 (ANO1)/TMEM16A is a Cl− channel activated by intracellular Ca2+ mediating numerous physiological functions. However, little is known of the ANO1 activation mechanism by Ca2+. Here, we demonstrate that two helices, “reference” and “Ca2+ sensor” helices in the third intracellular loop face each other with opposite charges. The two helices interact directly in a Ca2+-dependent manner. Positively and negatively charged residues in the two helices are essential for Ca2+-dependent activation because neutralization of these charges change the Ca2+ sensitivity. We now predict that the Ca2+ sensor helix attaches to the reference helix in the resting state, and as intracellular Ca2+ rises, Ca2+ acts on the sensor helix, which repels it from the reference helix. This Ca2+-dependent push-pull conformational change would be a key electromechanical movement for gating the ANO1 channel. Because chemical activation of ANO1 is viewed as an alternative means of rescuing cystic fibrosis, understanding its gating mechanism would be useful in developing novel treatments for cystic fibrosis. Electronic supplementary material The online version of this article (doi:10.1007/s00424-014-1603-2) contains supplementary material, which is available to authorized users.
- Published
- 2014
20. The cAMP-dependent kinase pathway does not sensitize the cloned vanilloid receptor type 1 expressed in Xenopus oocytes or Aplysia neurons
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Uhtaek Oh, Yongseok Lee, Jin-A Lee, Jooyoung Jung, and Bong-Kiun Kaang
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Patch-Clamp Techniques ,Phosphodiesterase Inhibitors ,Receptors, Drug ,Xenopus ,TRPV1 ,8-Bromo Cyclic Adenosine Monophosphate ,Gene Expression ,TRPV Cation Channels ,Transfection ,Membrane Potentials ,chemistry.chemical_compound ,Dorsal root ganglion ,1-Methyl-3-isobutylxanthine ,Aplysia ,medicine ,Animals ,Patch clamp ,Cloning, Molecular ,Protein kinase A ,Neurons ,Forskolin ,biology ,General Neuroscience ,Colforsin ,biology.organism_classification ,Cyclic AMP-Dependent Protein Kinases ,Molecular biology ,Cell biology ,medicine.anatomical_structure ,nervous system ,chemistry ,Oocytes ,Receptors, Adrenergic, beta-2 ,Neuron ,Capsaicin ,Signal Transduction - Abstract
Capsaicin-activated channels present in sensory neurons are ligand-gated cation channels that largely account for mediating some types of pain. The cAMP-dependent protein kinase (PKA) signal pathway was suggested to mediate the prostaglandin-induced enhancement of capsaicin-evoked inward current (I(CAP)) in rat sensory neurons. It is not clear, however, whether PKA acts directly on the capsaicin-sensitive channel that is responsible for I(CAP). To address this issue, we overexpressed the cloned capsaicin receptor, VR1, in heterologous expression systems such as Xenopus oocytes or Aplysia R2 neuron and stimulated PKA pathways. As a result, activation of PKA by applying either 8-bromo-cAMP or forskolin with 3-isobutyl-1-methylxanthine or through activation of beta(2) adrenergic receptors failed to enhance I(CAP) in oocytes or R2 neurons expressing VR1. Our results raise two possibilities. (1) Direct phosphorylation of VR1 by PKA may not be responsible for the sensitization; instead, phosphorylation of regulatory proteins associated with VR1 would account for the sensitization of I(CAP) evoked by prostaglandin E(2) in dorsal root ganglion (DRG) neurons. (2) DRG neurons may have a different PKA signaling mechanism that is not replicable in Xenopus oocytes or Aplysia R2 neurons.
- Published
- 2000
21. Direct activation of capsaicin receptors by products of lipoxygenases: Endogenous capsaicin-like substances
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Kyung-Hoon Min, Hawon Cho, Chang-Joong Kang, Uhtaek Oh, Sun Wook Hwang, Young-Ger Suh, Soohyun Cho, Jiyeon Kwak, Soon Youl Lee, Donghee Kim, and Jooyoung Jung
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Leukotrienes ,Lipid Peroxides ,Leukotriene B4 ,Receptors, Drug ,Lipoxygenase ,TRPV1 ,Endogeny ,Ligands ,Dinoprostone ,Vanilloids ,Cell Line ,Structure-Activity Relationship ,chemistry.chemical_compound ,Ganglia, Spinal ,Hydroxyeicosatetraenoic Acids ,Animals ,Humans ,Prostaglandins H ,Neurons, Afferent ,Receptor ,Cells, Cultured ,Inflammation ,Multidisciplinary ,Molecular Structure ,biology ,Prostaglandin D2 ,HEK 293 cells ,Biological Sciences ,Rats ,chemistry ,Biochemistry ,Capsaicin ,biology.protein ,Eicosanoids ,Prostaglandin H2 ,lipids (amino acids, peptides, and proteins) ,Ion Channel Gating - Abstract
Capsaicin, a pungent ingredient of hot peppers, causes excitation of small sensory neurons, and thereby produces severe pain. A nonselective cation channel activated by capsaicin has been identified in sensory neurons and a cDNA encoding the channel has been cloned recently. However, an endogenous activator of the receptor has not yet been found. In this study, we show that several products of lipoxygenases directly activate the capsaicin-activated channel in isolated membrane patches of sensory neurons. Among them, 12- and 15-( S )-hydroperoxyeicosatetraenoic acids, 5- and 15-( S )-hydroxyeicosatetraenoic acids, and leukotriene B 4 possessed the highest potency. The eicosanoids also activated the cloned capsaicin receptor (VR1) expressed in HEK cells. Prostaglandins and unsaturated fatty acids failed to activate the channel. These results suggest a novel signaling mechanism underlying the pain sensory transduction.
- Published
- 2000
22. A capsaicin-receptor antagonist, capsazepine, reduces inflammation-induced hyperalgesic responses in the rat: evidence for an endogenous capsaicin-like substance
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Jiyeon Kwak, Won Taek Lee, Sun Wook Hwang, Jooyoung Jung, and Uhtaek Oh
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Male ,medicine.drug_class ,Receptors, Drug ,Pain ,Pharmacology ,Carrageenan ,Functional Laterality ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Reflex ,medicine ,Animals ,Intradermal injection ,Inflammation ,Neurons ,General Neuroscience ,Antagonist ,Receptor antagonist ,Hindlimb ,Rats ,Nociception ,Spinal Cord ,chemistry ,Hyperalgesia ,Capsaicin ,Anesthesia ,medicine.symptom ,Capsazepine - Abstract
In the present study, the presence of an endogenous capsaicin-like substance and the role of capsaicin receptors in nociception during inflammation were assessed using Fos immunohistochemistry and the paw-withdrawal test in rats. Intradermal injection of carrageenan in the hind-paw produced inflammation in the foot pad, increased the number of cells exhibiting Fos-like immunoreactivity in the dorsal horn of the spinal cord, and decreased the paw-withdrawal latency. Intradermal injection of capsazepine, a capsaicin-receptor antagonist, significantly reduced the number of cells exhibiting Fos-like immunoreactivity, significantly increased the paw-withdrawal latency, but did not decrease inflammation induced by carrageenan injection. Intradermal injection of capsaicin or formalin also increased Fos-positive neurons. Capsaicin- or formalin-induced Fos expression was reduced in both cases by pretreatment of capsazepine, but to a much lesser extent for formalin. The capsazepine inhibition of carrageenan inflammation-induced hyperalgesic responses strongly suggests that an endogenous capsaicin-like substance is released in inflamed tissues and produces nociceptive neural impulses by acting on capsaicin receptors present on sensory neurons. Furthermore, our results indicate that capsaicin receptors take part only in generating nociceptive signals in sensory neurons, but not in activating the inflammation-promoting cells.
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- 1998
23. TRPM2 mediates the lysophosphatidic acid-induced neurite retraction in the developing brain
- Author
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Dong Jin Yang, Jooyoung Jung, Mi Hyun Lee, Boyoon Lee, Uhtaek Oh, Sunghoe Chang, Yongwoo Jang, Byung Woo Kim, Jesun Lee, Yasuo Mori, Hyeon Son, and Sung Hoon Lee
- Subjects
Neurite ,Physiology ,Neurogenesis ,Clinical Biochemistry ,TRPM Cation Channels ,Biology ,PC12 Cells ,Transient receptor potential channel ,chemistry.chemical_compound ,Mice ,Neurotrophic factors ,Physiology (medical) ,Lysophosphatidic acid ,Neurites ,Animals ,Humans ,TRPM2 ,Receptor ,Cells, Cultured ,Adenosine Diphosphate Ribose ,Activator (genetics) ,Brain ,Cell biology ,Rats ,HEK293 Cells ,nervous system ,chemistry ,Lysophospholipids ,Neuroscience ,Intracellular - Abstract
Intracellular Ca(2+) signal is a key regulator of axonal growth during brain development. As transient receptor potential (TRP) channels are permeable to Ca(2+) and mediate numerous brain functions, it is conceivable that many TRP channels would regulate neuronal differentiation. We therefore screened TRP channels that are involved in the regulation of neurite growth. Among the TRP channels, the Trpm2 level was inversely associated with neurite growth. TRPM2 was highly expressed in embryonic brain. Pharmacological perturbation or knockdown of TRPM2 markedly increased the axonal growth, whereas its overexpression inhibited the axonal growth. Addition of ADP ribose, an endogenous activator of TRPM2, to PC12 cells significantly repressed the axonal growth. TRPM2 was actively involved in the neuronal retraction induced by cerebrospinal fluid-rich lysophosphatidic acid (LPA). More importantly, neurons isolated from the brain of Trpm2-deficient mice have significantly longer neurites with a greater number of spines than those obtained from the brain of wild-type mice. Therefore, we conclude that TRPM2 mediates the LPA-induced suppression of axonal growth, which provides a long-sought mechanism underlying the effect of LPA on neuronal development.
- Published
- 2013
24. Computational complexity scalable scheme for power-aware H.264/AVC encoding
- Author
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Byeungwoo Jeon, Bongsoo Jung, Ho-Young Lee, and Jooyoung Jung
- Subjects
Computational complexity theory ,Computer engineering ,Computer science ,Motion estimation ,Real-time computing ,Scalability ,Worst-case complexity ,Workload ,Computational resource ,Encoder ,Coding (social sciences) - Abstract
Recent development of portable devices and prevalence of high-throughput communication infrastructures make the video encoding in portable devices on high demand. However, its computational complexity makes the implementation of realtime video encoder on portable devices extremely difficult. Many fast algorithms to solve the problems are not efficient from the view point of worst workload since they consider only reduction of average computational complexity. Moreover, since the amount of reduction of computational complexity highly depends on video sequence, fixed fast algorithms cannot always achieve their full potential for real-time encoding. In this paper, we analyze the complexity of H.264/AVC video coding tools, and develop two parameters for complexity control. Consequently, we design a power-aware complexity scalable encoding scheme implementable on embedded system. Using our target embedded system, the proposed method is verified to save power by about 50%.
- Published
- 2009
25. Fast subpel motion estimation using selective motion vector accuracy of inter-mode decision for H.264/AVC
- Author
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Byeungwoo Jeon, Yong-Serk Kim, Bongsoo Jung, Woong Choi, Jooyoung Jung, and Dae-Sung Cho
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Motion compensation ,business.industry ,Computer science ,Motion estimation ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Computer vision ,Artificial intelligence ,business ,Motion vector ,Transform coding ,Quarter-pixel motion - Abstract
MPEG-4 Part-10 AVC/H.264 (H.264/AVC) is the newest video coding standard developed and finalized in March 2003 by the Joint Video Team (JVT) of ISO/IEC (MPEG) and ITU-T (VCEG). It includes many powerful features such as multiple reference pictures, variable block-size motion compensation, quarter-pel accuracy motion compensation, and so on. As a result, it achieves high coding performance but at the expense of huge computational complexity. To reduce the complexity while keeping the same coding performance, we propose a low complexity subpel motion estimation method using two content-aware decision criteria. Based on the criteria, four different levels of subpel motion estimation are proposed to achieve low complexity subpel motion estimation. Simulation results show that the proposed method reduces total encoding time by 55.53% on average and subpel motion estimation time by 82.2% on average.
- Published
- 2009
26. Non-small cell lung cancer initially presenting with intracardiac metastasis
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Sang Ik Hwang, Chull Sung Jung, Sang Hak Lee, Young Iee Park, Jung Han Kim, Jooyoung Jung, and Chong Woo Yoo
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Male ,medicine.medical_specialty ,Pathology ,Lung Neoplasms ,Heart Ventricles ,Case Report ,Malignancy ,Intracardiac injection ,Metastasis ,Heart Neoplasms ,Carcinoma, Non-Small-Cell Lung ,Carcinoma ,Medicine ,Humans ,cardiovascular diseases ,Lung cancer ,Lymph node ,Cardiac metastasis ,Lung ,business.industry ,Middle Aged ,medicine.disease ,medicine.anatomical_structure ,PET ,Ventricle ,cardiovascular system ,Radiology ,business - Abstract
Intracardiac metastasis as the initial presentation of malignant neoplasm is very rare. We report here on a 64-year-old man with non-small cell lung cancer (NSCLC) initially presenting with intracardiac metastasis which was identified with 18-F fluorodeoxyglucose positron emission tomography (FDG PET). The patient was admitted with complaints of exertional dyspnea and vague chest discomfort that had developed a few weeks ago. Two-dimensional echocardiography revealed a heart mass attached to its akinetic wall in the right ventricular chamber. CT and MRI demonstrated a large tumor involving the epicardium and myocardium in the right ventricle, and there was a mass in the right lower lobe of the lung along with multiple lymphadenopathies. Cytologic examination of the percutaneous needle aspiration of a lymph node in the anterior mediastinum revealed malignant epithelial cell nests, and this was strongly suggestive of squamous cell carcinoma. Subsequent FDG PET confirmed that the intracardiac mass had an abnormally increased FDG uptake, and again this was strongly suggestive of malignancy. By systemically considering these imaging studies, we were able to diagnose the mass as intracardiac metastasis of NSCLC.
- Published
- 2005
27. Jumonji regulates cardiomyocyte proliferation via interaction with retinoblastoma protein
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Hyeong Reh Choi Kim, Tae-gyun Kim, Jooyoung Jung, Gary E. Lyons, and Youngsook Lee
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Recombinant Fusion Proteins ,Nerve Tissue Proteins ,Biochemistry ,Retinoblastoma Protein ,Mice ,Cyclin D1 ,Cyclin D2 ,Genes, Reporter ,Cyclins ,Animals ,Humans ,Myocytes, Cardiac ,E2F ,Promoter Regions, Genetic ,Molecular Biology ,Psychological repression ,Cells, Cultured ,Cell Proliferation ,Cyclin-dependent kinase 1 ,biology ,Cell growth ,Cell Cycle ,Retinoblastoma protein ,Polycomb Repressive Complex 2 ,Cell Biology ,Cell cycle ,Embryo, Mammalian ,Molecular biology ,Cell biology ,Protein Structure, Tertiary ,Phenotype ,Gene Expression Regulation ,biology.protein - Abstract
Jumonji (JMJ) can function as a transcriptional repressor and plays critical roles in embryonic development including heart development in mice. Although JMJ has been suggested to play a role in cell growth, the molecular mechanisms have not been resolved. The present data demonstrate that JMJ interacts with the retinoblastoma protein (Rb), one of the master regulatory genes of cell cycle. JMJ potentiates the repression function of Rb on E2F activities, leading to reduced cell cycle progression. The transcriptional repression domain of JMJ is critical for the interaction with Rb as well as repression of cell cycle. The physiological relevance of the association between Rb and JMJ was assessed in cardiomyocytes. Primary cardiomyocytes cultured from homozygous jmj knock-out mouse embryos (jmj mutants) show increased cell mitosis in a cardiomyocyte-specific manner. Reporter gene analyses demonstrate that promoter activities of cyclin D1, cyclin D2, and Cdc2 are up-regulated in jmj mutant cardiomyocytes. These data suggest that JMJ down-regulates the cell growth via interaction with Rb, which would provide important insights into the cardiac defects observed in jmj mutant mice.
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- 2005
28. Jumonji represses alpha-cardiac myosin heavy chain expression via inhibiting MEF2 activity
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Matthew R. Mysliwiec, Seog-Youn Kang, Jooyoung Jung, Youngsook Lee, and Tae-gyun Kim
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Gene isoform ,Mef2 ,Biophysics ,Endogeny ,Nerve Tissue Proteins ,Biology ,Biochemistry ,Mice ,Myosin ,Animals ,Myocytes, Cardiac ,Cloning, Molecular ,Molecular Biology ,Gene ,Psychological repression ,Transcription factor ,Cells, Cultured ,Thyroid hormone receptor ,Myosin Heavy Chains ,MEF2 Transcription Factors ,Polycomb Repressive Complex 2 ,Gene Expression Regulation, Developmental ,Cell Biology ,Molecular biology ,Recombinant Proteins ,Rats ,DNA-Binding Proteins ,Animals, Newborn ,Myogenic Regulatory Factors ,Transcription Factors - Abstract
Expression of alpha-cardiac myosin heavy chain gene (alphaMHC) is developmentally regulated in normal embryonic hearts and down-regulated in cardiac myopathy and failing hearts. Jumonji (JMJ) has been shown to be critical for normal cardiovascular development and functions as a transcriptional repressor. Here, we demonstrate that JMJ represses alphaMHC expression through inhibition of myocyte enhancer factor 2 (MEF2) activity. In primary cardiomyocytes, overexpression of JMJ leads to marked reduction of endogenous alphaMHC expression. JMJ represses the synergistic activation of alphaMHC by MEF2 and thyroid hormone receptor (TR). Interestingly, JMJ inhibits transcriptional activities of all MEF2 isoforms, but not the TR-dependent activation. The transcriptional repression domain of JMJ interacts with the N-terminal part of MEF2A, resulting in the repression of MEF2A activities. These results suggest that JMJ represses alphaMHC expression via protein-protein interaction with MEF2A.
- Published
- 2005
29. Roles of JUMONJI in mouse embryonic development
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Youngsook Lee, Matthew R. Mysliwiec, and Jooyoung Jung
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Models, Anatomic ,Heterozygote ,Time Factors ,Transcription, Genetic ,Morphogenesis ,Embryonic Development ,Nerve Tissue Proteins ,Biology ,Cardiovascular System ,Models, Biological ,Mice ,Transcription (biology) ,Cell Movement ,medicine ,Transcriptional regulation ,Animals ,Gene ,Transcription factor ,Regulation of gene expression ,Genetics ,Cell Nucleus ,Embryogenesis ,Cell Cycle ,Homozygote ,Neural tube ,Polycomb Repressive Complex 2 ,Gene Expression Regulation, Developmental ,Cell biology ,Protein Structure, Tertiary ,medicine.anatomical_structure ,Liver ,Neural Crest ,Developmental Biology ,Transcription Factors - Abstract
Cardiac development is a complex biological process requiring the integration of cell specification, differentiation, migration, proliferation, and morphogenesis. Although significant progress has been made recently in understanding the molecular basis of cardiac development, mechanisms of transcriptional control of cardiac development remain largely unknown. In search for the developmentally important genes, the jumonji gene (jmj) was identified by gene trap technology and characterized as a critical nuclear factor for mouse embryonic development. Jmj has been shown to play important roles in cardiovascular development, neural tube fusion process, hematopoiesis, and liver development in mouse embryos. The amino acid sequence of the JUMONJI protein (JMJ) reveals that JMJ belongs to the AT-rich interaction domain transcription factor family and more recently has been described as a member of the JMJ transcription factor family. Here, we review the roles of jmj in multiple organ development with a focus on cardiovascular development in mice.
- Published
- 2004
30. Phosphorylation of vanilloid receptor 1 by Ca2+/calmodulin-dependent kinase II regulates its vanilloid binding
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Jaeyeon Koo, Soon Youl Lee, Sun Wook Hwang, Jooyoung Jung, Hawon Cho, Uhtaek Oh, and Jae Soo Shin
- Subjects
Receptors, Drug ,Immunoblotting ,Resiniferatoxin ,TRPV1 ,Ligands ,Biochemistry ,Models, Biological ,Cell Line ,Dephosphorylation ,chemistry.chemical_compound ,Cytosol ,Ca2+/calmodulin-dependent protein kinase ,Animals ,Humans ,Point Mutation ,Biotinylation ,Phosphorylation ,Protein kinase A ,Molecular Biology ,Cells, Cultured ,Protein Kinase C ,Neurons ,Binding Sites ,Chemistry ,Kinase ,Cell Biology ,Interleukin-13 receptor ,Cyclic AMP-Dependent Protein Kinases ,Cell biology ,Protein Structure, Tertiary ,Rats ,Kinetics ,Calcium-Calmodulin-Dependent Protein Kinases ,Mutation ,Mutagenesis, Site-Directed ,Oocytes ,Capsaicin ,Diterpenes ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,Protein Binding - Abstract
Vanilloid receptor 1 (VR1), a capsaicin receptor, is known to play a major role in mediating inflammatory thermal nociception. Although the physiological role and biophysical properties of VR1 are known, the mechanism of its activation by ligands is poorly understood. Here we show that VR1 must be phosphorylated by Ca2+-calmodulin dependent kinase II (CaMKII) before its activation by capsaicin. In contrast, the dephosphorylation of VR1 by calcineurin leads to a desensitization of the receptor. Moreover, point mutations in VR1 at two putative consensus sites for CaMKII failed to elicit capsaicin-sensitive currents and caused a concomitant reduction in VR1 phosphorylation in vivo. Such mutants also lost their high affinity binding with [3H]resiniferatoxin, a potent capsaicin receptor agonist. We conclude that the dynamic balance between the phosphorylation and dephosphorylation of the VR1 channel by CaMKII and calcineurin, respectively, controls the activation/desensitization states by regulating VR1 binding. Furthermore, because sensitization by protein kinase A and C converge at these sites, phosphorylation stress in the cell appears to control a wide range of excitabilities in response to various adverse stimuli.
- Published
- 2003
31. Induction chemotherapy followed by radiotherapy in the treatment of anal cancer
- Author
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Noe Kyeong Kim, Dae Ho Lee, Sung Whan Ha, Jooyoung Jung, Inho Kim, Keun Wook Lee, Dae-Young Kim, Hun Ho Song, Jae-Gahb Park, Tae-You Kim, Dae Seog Heo, and Yung-Jue Bang
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,medicine.drug_class ,Mitomycin ,medicine.medical_treatment ,Antimetabolite ,Cohort Studies ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Medicine ,Anal cancer ,Survival rate ,Aged ,Neoplasm Staging ,Retrospective Studies ,Chemotherapy ,business.industry ,Remission Induction ,Cancer ,Induction chemotherapy ,General Medicine ,Middle Aged ,Anus Neoplasms ,medicine.disease ,Combined Modality Therapy ,Surgery ,Survival Rate ,Radiation therapy ,Oncology ,Fluorouracil ,Carcinoma, Squamous Cell ,Female ,Cisplatin ,business ,Follow-Up Studies ,medicine.drug - Abstract
This study was conducted to investigate the efficacy of the induction chemotherapy followed by radiotherapy in anal cancer. Twenty-three patients diagnosed with anal cancer between March 1991 and February 1999 were treated with induction chemotherapy with 5-fluorouracil based regimens followed by external beam radiation. After a median follow-up of 78 months, the overall survival and the disease-free survival at 5 years was 71.3 and 67.5%, respectively. The colostomy-free survival at 5 years was 91%, as the inguinal lymph nodes were the most frequent site of relapse. Serious side effects did not occur and late complications did not develop either. Induction chemotherapy followed by radiotherapy in patients with anal cancer is effective in terms of its response and preserving the anal sphincter without serious acute and late complications.
- Published
- 2003
32. Bradykinin-12-lipoxygenase-VR1 signaling pathway for inflammatory hyperalgesia
- Author
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Soon Youl Lee, David B. Reichling, Chan Young Shin, Uhtaek Oh, Jinwoong Kim, Nicole Alessandri Haber, Sachia G. Khasar, Hawon Cho, So Hee Kim, Ji-eun Shin, Myung Gull Lee, Jon D. Levine, Sun Wook Hwang, Young Hae Choi, and Jooyoung Jung
- Subjects
medicine.medical_specialty ,Leukotrienes ,Receptors, Drug ,Neurotoxins ,Bradykinin ,Pain ,Inflammation ,Pharmacology ,Arachidonate 12-Lipoxygenase ,Transfection ,Cell Line ,chemistry.chemical_compound ,Lipoxygenase ,Internal medicine ,Ganglia, Spinal ,medicine ,Animals ,Humans ,Neurons, Afferent ,Receptor ,Cells, Cultured ,chemistry.chemical_classification ,Neurons ,Multidisciplinary ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Receptors, Bradykinin ,Fatty acid ,Biological Sciences ,Rats ,Endocrinology ,chemistry ,Animals, Newborn ,Hyperalgesia ,biology.protein ,Arachidonic acid ,medicine.symptom ,Signal transduction ,Diterpenes ,Signal Transduction - Abstract
The capsaicin-sensitive vanilloid receptor (VR1) was recently shown to play an important role in inflammatory pain (hyperalgesia), but the underlying mechanism is unknown. We hypothesized that pain-producing inflammatory mediators activate capsaicin receptors by inducing the production of fatty acid agonists of VR1. This study demonstrates that bradykinin, acting at B2 bradykinin receptors, excites sensory nerve endings by activating capsaicin receptors via production of 12-lipoxygenase metabolites of arachidonic acid . This finding identifies a mechanism that might be targeted in the development of new therapeutic strategies for the treatment of inflammatory pain.
- Published
- 2002
33. An Adult with Aplastic Crisis induced by Human Parvovirus B19 as an Initial Presentation of Hereditary Spherocytosis
- Author
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Jung Han Kim, Kwang Hyuk Park, Chi Hun Choi, Min Jeong Park, Sook Eui Oh, Jooyoung Jung, and Young Lee Park
- Subjects
Adult ,Anemia ,viruses ,Aplastic crisis ,Spherocytosis ,Case Report ,Spherocytosis, Hereditary ,Hereditary spherocytosis ,Parvoviridae Infections ,hemic and lymphatic diseases ,Parvovirus B19, Human ,medicine ,Humans ,Reticulocytopenia ,Parvovirus B 19 ,business.industry ,Anemia, Aplastic ,virus diseases ,medicine.disease ,Pancytopenia ,Sickle cell anemia ,medicine.anatomical_structure ,Immunology ,Female ,Bone marrow ,Packed red blood cells ,business ,Pancyopenia - Abstract
The association between aplastic crisis and human parvovirus (HPV) B19 infection is well described in patients with sickle cell anemia. This association has also been described, although much less frequently, in patients with hereditary spherocytosis (HS). However, most cases of aplastic crises in patients with HS and induced by HPV B19 have been reported in children or adolescents. In this paper, we describe an aplastic crisis induced by HPV B19 in an adult with HS. A 34-year-old female presented with presyncope, febrile sensation, and myalgia. The complete blood counts showed severe anemia. The peripheral blood smear revealed spherocytosis with reticulocytopenia and pancytopenia. The direct Coombs' test was negative; the osmotic fragility test was positive. In the bone marrow aspirates, a few giant pronormoblasts with deep blue cytoplasm, pseudopods, and intracellular inclusion bodies were observed. The patient was given eight units of packed red blood cells. HPV B19 infection was proven by the presence of IgM antibodies to HPV B19 and the detection of viral DNA using the PCR technique. To the best of our knowledge, this is the first report in Korea that describes an adult with aplastic crisis presenting initially with HS.
- Published
- 2005
34. A Case of Acute Mediastinitis Associated with Fish Bone with Successful Conservative Treatment
- Author
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Byung-Cheol Song, Hong Sup Lee, Jooyoung Jung, Guk Myung Choi, Sun Wook Kim, Jin Su Hwang, Kyung Mook Kim, Chung Ho Yeum, and An Soo Jang
- Subjects
Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,business.industry ,Optimal treatment ,medicine.disease ,Mediastinitis ,Surgery ,Conservative treatment ,Infectious Diseases ,Swallowing ,medicine ,Severe morbidity ,business ,Intensive care medicine ,Order perciformes ,Acute mediastinitis ,Fish bone - Abstract
Acute mediastinitis is a life threatening disorder that causes severe morbidity in affected patient. We experienced a case of acute mediastinitis in a 56 year-old man as a result of swallowing a fish bone (order Perciformes, Family Pomacentridae). Conservative treatment, including starvation therapy and the injection of antibiotic drugs, were successful in treating this patient. Although the optimal treatment for acute mediastinitis remains controversial, it is important to choose the appropriate strategies when encountering cases of acute mediastinitis.
- Published
- 2002
35. Cellular functions of TMEM16/anoctamin
- Author
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Uhtaek Oh and Jooyoung Jung
- Subjects
0301 basic medicine ,Nociception ,medicine.medical_specialty ,Phospholipid scramblase ,TMEM16 ,Carcinogenesis ,Physiology ,Proliferation ,Clinical Biochemistry ,Gating ,ANO1 ,03 medical and health sciences ,Ca2+-activated Cl− channel ,Chlorides ,Scott syndrome ,Chloride Channels ,Internal medicine ,Physiology (medical) ,medicine ,Scramblase ,Animals ,Humans ,Secretion ,Receptor ,Cl− secretion ,Invited Review ,Ion Transport ,biology ,Phospholipid transport ,medicine.disease ,030104 developmental biology ,Endocrinology ,Anoctamin ,Tumorigenesis ,Chloride channel ,biology.protein ,Calcium ,Signal Transduction - Abstract
Ca(2+)-activated Cl(-) channels (CaCCs) are a class of Cl(-) channels activated by intracellular Ca(2+) that are known to mediate numerous physiological functions. In 2008, the molecular identity of CaCCs was found to be anoctamin 1 (ANO1/TMEM16A). Its roles have been studied in electrophysiological, histological, and genetic aspects. ANO1 is known to mediate Cl(-) secretion in secretory epithelia such as airways, salivary glands, intestines, renal tubules, and sweat glands. ANO1 is a heat sensor activated by noxious heat in somatosensory neurons and mediates acute pain sensation as well as chronic pain. ANO1 is also observed in vascular as well as airway smooth muscles, controlling vascular tone as well as airway hypersensitivity. ANO1 is upregulated in numerous types of cancers and thus thought to be involved in tumorigenesis. ANO1 is also found in proliferating cells. In addition to ANO1, involvement of its paralogs in pathophysiological conditions was also reported. ANO2 is involved in olfaction, whereas ANO6 works as a scramblase whose mutation causes a rare bleeding disorder, the Scott syndrome. ANO5 is associated with muscle and bone diseases. Recently, an X-ray crystal structure of a fungal TMEM16 was reported, which explains a precise molecular gating mechanism as well as ion conduction or phospholipid transport across the plasma membrane.
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36. Anoctamin 1 Contributes to Inflammatory and Nerve-Injury Induced Hypersensitivity
- Author
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Young Duk Yang, Uhtaek Oh, Dong-Jin Yang, Byeongjun Lee, Jooyoung Jung, and Hawon Cho
- Subjects
Nociception ,Inflammatory pain ,ANO1 ,Inflammation ,Bradykinin ,Neuropathic pain ,Cellular and Molecular Neuroscience ,Mice ,Chloride Channels ,Formaldehyde ,medicine ,Noxious stimulus ,Hypersensitivity ,Animals ,RNA, Messenger ,DRG neuron ,Anoctamin-1 ,Mice, Knockout ,biology ,business.industry ,Research ,Rheobase ,Nerve injury ,Sciatic Nerve ,Allodynia ,Anesthesiology and Pain Medicine ,Gene Expression Regulation ,nervous system ,Hyperalgesia ,Organ Specificity ,Anesthesia ,biology.protein ,Molecular Medicine ,medicine.symptom ,business ,Neuroscience - Abstract
Background Various pathological conditions such as inflammation or injury can evoke pain hypersensitivity. That represents the response to innocuous stimuli or exaggerated response to noxious stimuli. The molecular mechanism based on the pain hypersensitivity is associated with changes in many of ion channels in dorsal-root ganglion (DRG) neurons. Anoctamin 1 (ANO1/TMEM16A), a Ca2+ activated chloride channel is highly visible in small DRG neurons and responds to heat. Mice with an abolished function of ANO1 in DRG neurons demonstrated attenuated pain-like behaviors when exposed to noxious heat, suggesting a role in acute thermal nociception. In this study, we further examined the function of ANO1 in mediating inflammation- or injury-induced hyperalgesia or allodynia. Results Using Advillin/Ano1 fl/fl (Adv/Ano1 fl/fl ) mice that have a functional ablation of Ano1 mainly in DRG neurons, we were able to determine its role in mediating thermal hyperalgesia and mechanical allodynia induced by inflammation or nerve injury. The thermal hyperalgesia and mechanical allodynia induced by carrageenan injection and spared-nerve injury were significantly reduced in Adv/Ano1 fl/fl mice. In addition, flinching or licking behavior after bradykinin or formalin injection was also significantly reduced in Adv/Ano1 fl/fl mice. Since pathological conditions augment nociceptive behaviors, we expected ANO1′s contribution to the excitability of DRG neurons. Indeed, the application of inflammatory mediators reduced the threshold for action potential (rheobase) or time for induction of the first action potential in DRG neurons isolated from control (Ano1 fl/fl ) mice. These parameters for neuronal excitability induced by inflammatory mediators were not changed in Adv/Ano1 fl/fl mice, suggesting an active contribution of ANO1 in augmenting the neuronal excitability. Conclusions In addition to ANO1's role in mediating acute thermal pain as a heat sensor, ANO1 is also capable of augmenting the excitability of DRG neurons under inflammatory or neuropathic conditions and thereby aggravates inflammation- or tissue injury-induced pathological pain.
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37. Capsaicin binds to the intracellular domain of the capsaicin-activated ion channel
- Author
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Chang Joong Kang, Soon Youl Lee, Won Bae Kim, Jooyoung Jung, Sun Wook Hwang, Jiyeon Kwak, Uhtaek Oh, and Donghee Kim
- Subjects
Patch-Clamp Techniques ,medicine.drug_class ,Dopamine ,TRPV1 ,Article ,Ion Channels ,Membrane Potentials ,chemistry.chemical_compound ,medicine ,Extracellular ,Animals ,Receptor ,Cells, Cultured ,Ion channel ,Neurons ,Binding Sites ,General Neuroscience ,Cell Membrane ,Receptor antagonist ,Electric Stimulation ,Rats ,Electrophysiology ,Animals, Newborn ,chemistry ,Biochemistry ,Oocytes ,Biophysics ,Capsaicin ,Capsazepine ,Intracellular ,Signal Transduction ,Binding domain - Abstract
Capsaicin (CAP) excites small sensory neurons, causing pain, neurogenic inflammation, and other visceral reflexes. These effects have been proposed to be the result of CAP activation of a nonselective cation current. It is generally assumed that CAP binds to an extracellular domain of the membrane receptor. However, the exact binding site is not known because of the lipophilic nature of CAP. To determine whether the binding domain is extracellular or intracellular, we tested the effect of a synthetic water-soluble CAP analog, DA-5018·HCl, on current activation. CAP activated the 45 pS (at −60 mV) nonselective cation channel from either side of the membrane. However, DA-5018·HCl, which had a greater potency and efficacy than CAP, activated the channels only from the cytosolic side of the patch membrane in a capsazepine, a CAP receptor antagonist, reversible manner. When applied extracellularly, DA-5018·HCl did not, but CAP did, activate whole-cell currents in sensory neurons, as well as in oocytes expressing vanilloid receptor 1, a recently cloned CAP receptor. Hydrogen ions, reported as a possible endogenous activator of cation current, failed to elicit any current when acidic medium (pH 5.0–6.0) was applied intracellularly, indicating that H+does not mediate the CAP effect. These results indicate that CAP and its analog bind to the cytosolic domain of the CAP receptor and suggest that an endogenous CAP-like substance other than H+may be present in the cell.
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