Back to Search
Start Over
Direct activation of capsaicin receptors by products of lipoxygenases: Endogenous capsaicin-like substances
- Source :
- Proceedings of the National Academy of Sciences. 97:6155-6160
- Publication Year :
- 2000
- Publisher :
- Proceedings of the National Academy of Sciences, 2000.
-
Abstract
- Capsaicin, a pungent ingredient of hot peppers, causes excitation of small sensory neurons, and thereby produces severe pain. A nonselective cation channel activated by capsaicin has been identified in sensory neurons and a cDNA encoding the channel has been cloned recently. However, an endogenous activator of the receptor has not yet been found. In this study, we show that several products of lipoxygenases directly activate the capsaicin-activated channel in isolated membrane patches of sensory neurons. Among them, 12- and 15-( S )-hydroperoxyeicosatetraenoic acids, 5- and 15-( S )-hydroxyeicosatetraenoic acids, and leukotriene B 4 possessed the highest potency. The eicosanoids also activated the cloned capsaicin receptor (VR1) expressed in HEK cells. Prostaglandins and unsaturated fatty acids failed to activate the channel. These results suggest a novel signaling mechanism underlying the pain sensory transduction.
- Subjects :
- Leukotrienes
Lipid Peroxides
Leukotriene B4
Receptors, Drug
Lipoxygenase
TRPV1
Endogeny
Ligands
Dinoprostone
Vanilloids
Cell Line
Structure-Activity Relationship
chemistry.chemical_compound
Ganglia, Spinal
Hydroxyeicosatetraenoic Acids
Animals
Humans
Prostaglandins H
Neurons, Afferent
Receptor
Cells, Cultured
Inflammation
Multidisciplinary
Molecular Structure
biology
Prostaglandin D2
HEK 293 cells
Biological Sciences
Rats
chemistry
Biochemistry
Capsaicin
biology.protein
Eicosanoids
Prostaglandin H2
lipids (amino acids, peptides, and proteins)
Ion Channel Gating
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....3a2cd835c9ef59a4c37da74bf5305012