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Made available in DSpace on 2019-01-23T00:23:44Z (GMT). No. of bitstreams: 1 ART18110.pdf: 7581880 bytes, checksum: 89cf25bc807424ab4826cf63f2b0b9d9 (MD5) Previous issue date: 2019-01-17

Breeding of caffeine-free coffee cultivars require tools for an early selection of progenies bearing this later trait. Genes from caffeine synthesis and degradation represent major targets for the development of molecular markers for assisted selection. In this study, we characterized SNPs identified on the caffeine synthase gene from AC1 mutant, a naturally caffeine-free arabica coffee plant. Molecular analysis of normal and mutant sequences indicates the occurrence of SNPs in protein domains, potentially associated with caffeine synthesis in coffee. Progenies F2, F1BC1 and BC from crosses of AC mutants and elite cultivars were evaluated regarding caffeine content in grains and genomic segregation profile of selected SNPs. Genotyping analysis allowed the discrimination between homozygous and heterozygous plants. Quantification of caffeine content indicated a significant variability among progenies and a low frequency of caffeine-free plants. Statistical analyses of genotyping and phenotyping results showed significant association between presence of selected SNPs and reduced caffeine content. Moreover, this association occurs through all evaluated genetic backgrounds and generations, indicating an inheritance stability of both trait and markers. The molecular markers described here represent a successful case of assistedselection in coffee, indicating their potential use for breeding of caffeine-free cultivars. Made available in DSpace on 2019-10-19T00:34:01Z (GMT). No. of bitstreams: 1 Assistedselecctionofnaturally.pdf: 1046854 bytes, checksum: 8820cae6d40264b70f472122655259b2 (MD5) Previous issue date: 2017

Genome wide selection (GWS) is essential for the genetic improvement of perennial species such as Citrus because of its ability to increase gain per unit time and to enable the efficient selection of characteristics with low heritability. This study assessed GWS efficiency in a population of Citrus and compared it with selection based on phenotypic data. A total of 180 individual trees from a cross between Pera sweet orange (Citrus sinensis Osbeck) and Murcott tangor (Citrus sinensis Osbeck x Citrus reticulata Blanco) were evaluated for 10 characteristics related to fruit quality. The hybrids were genotyped using 5287 DArT_seqTM (diversity arrays technology) molecular markers and their effects on phenotypes were predicted using the random regression - best linear unbiased predictor (rr-BLUP) method. The predictive ability, prediction bias, and accuracy of GWS were estimated to verify its effectiveness for phenotype prediction. The proportion of genetic variance explained by the markers was also computed. The heritability of the traits, as determined by markers, was 16-28%. The predictive ability of these markers ranged from 0.53 to 0.64, and the regression coefficients between predicted and observed phenotypes were close to unity. Over 35% of the genetic variance was accounted for by the markers. Accuracy estimates with GWS were lower than those obtained by phenotypic analysis; however, GWS was superior in terms of genetic gain per unit time. Thus, GWS may be useful for Citrus breeding as it can predict phenotypes early and accurately, and reduce the length of the selection cycle. This study demonstrates the feasibility of genomic selection in Citrus. Made available in DSpace on 2018-01-03T23:18:49Z (GMT). No. of bitstreams: 1 2016M.DeonGMRGenome.pdf: 563165 bytes, checksum: fddf34ba0f27a1535a688be01db2966b (MD5) Previous issue date: 2017-07-14

O objetivo deste trabalho foi avaliar o acúmulo de matéria seca e de nutrientes de plantas forrageiras consorciadas com o milho safrinha, em função da adubação nitrogenada. As espécies Urochloa brizantha cv. Marandu, U. decumbens cv. Basilisk, U. ruziziensis cv. Comum e Panicum maximum cv. Tanzânia, com semeadura em meados de março, foram avaliadas em quatro municípios paulistas, em consórcio com o milho. Utilizou-se o delineamento experimental de blocos ao acaso, com quatro repetições, em parcelas subdivididas. Nas parcelas, foram avaliados os consórcios e nas subparcelas, quatro doses de nitrogênio em cobertura (0, 30, 60 e 90 kg ha-1). Foi determinado o acúmulo de matéria seca e de nutrientes pelas plantas forrageiras em três épocas: no florescimento, na maturidade fisiológica do milho e por ocasião da dessecação das forrageiras, em outubro. O acúmulo de matéria seca das forrageiras intensifica-se após a colheita do milho safrinha. Quando as forrageiras são semeadas na entrelinha do milho, a adubação nitrogenada em cobertura não interfere no acúmulo de matéria seca e de nutrientes pelas forrageiras, e o consórcio não afeta a produtividade de grãos de milho safrinha. The objective of this work was to assess the accumulation of dry matter and nutrients in forage plants intercropped with off-season maize, as a function of nitrogen fertilization. The species Urochloa brizantha cv. Marandu, U. decumbens cv. Basilisk, U. ruziziensis cv. Comum, and Panicum maximum cv. Tanzânia, with seeding in mid-March, were evaluated in four municipalities of São Paulo, Brazil, intercropped with maize. A randomized complete block design was used, with four replicates, in split-plots. The intercropped systems were evaluated in the plots, and four nitrogen topdressing fertilization rates (0, 30, 60, and 90 kg ha-1) were evaluated in the split-plots. Forage dry matter and nutrient accumulation were determined in three stages: flowering, maize physiological maturity, and at forage desiccation, in October. Forage dry matter accumulation increases after the harvest of off-season maize. When forages are planted in the corn inter-rows, topdressing nitrogen fertilization does not influence forage dry matter and nutrient accumulation, and intercropping does not affect off-season maize grain yield.

Polyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contributions of each subgenome in C. arabica. In silico data were validated by the quantitative polymerase chain reaction and allele-specific combination TaqMAMA-based method. The presence of differential expression of C. arabica homeologous genes and its implications in coffee gene expression, ontology, and physiology are discussed.

Este trabalho teve como objetivo avaliar in vitro a reação de porta-enxertos de citros (Citrus spp.) a Phytophthora parasitica. As plântulas foram cultivadas em meio MS por 40 dias sendo, submetidas a fotoperíodo de 18 h, à temperatura de 25 ºC. A inoculação foi realizada através da inserção de agulha infestada com micélio de P. parasitica. A avaliação foi realizada aos 15 dias após a inoculação, medindo-se o comprimento das lesões em centímetros. O delineamento experimental foi inteiramente casualizado, com 15 repetições. Os resultados estão de acordo com as reações de campo dos genótipos e podem ser de grande utilidade em trabalhos envolvendo resistência varietal e seleção precoce de plantas. The objective of this work was to evaluate the effect on citrus (Citrus spp.) rootstocks to Phytophthora parasitica by in vitro inoculation. Seedlings were cultivated in MS media for 40 days, under 18 h of light of photoperiod and at 25 ºC. Inoculation was performed by injecting an infested needle into seedling collars. Evaluation was made by the length of lesions in centimeters. The experimental design was random with 15 replicates of each genotype. Results indicate good correlation between in vitro and orchard reactions genotypes to P. parasitica.

Made available in DSpace on 2022-04-28T19:45:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Sugarcane yellow leaf virus (ScYLV), Polerovirus, Luteoviridae, is one of the main viruses that infect sugarcane worldwide. The virus is transmitted by the aphid Melanaphis sacchari in a persis-tent, circulative manner. To better understand the interactions between ScYLV, sugarcane geno-types and M. sacchari, we explored the effect of sugarcane cultivars on the feeding behavior and biological performance of the vector. The number of nymphs, adults, winged, total number of aphids and dead aphids was assayed, and an electrical penetration graph (EPG) was used to mon-itor the stylet activities. Multivariate analysis showed changes in the vector’s behavior and biology on cultivars, identifying specific groups of resistance. In the cultivar 7569, only 5.5% of the insects were able to stay longer on sustained phloem ingestion, while in the other seven cultivars these values varied from 20% to 60%. M. sacchari showed low phloem activities in cultivars 7569 and Bio266. Overall, cultivar 7569 showed the worst biological performance of aphids, with the insects presenting mechanical difficulties for feeding and a shorter duration of the phloem period, and thus being considered the most resistant. We conclude that ScYLV virus infection in different sugarcane cultivars induced specific changes in the host plant, modifying the behavior of its main vector, which may favor or impair virus transmission. School of Agricultural and Veterinarian Sciences—FCAV São Paulo State University—UNESP Dep. of Entomology and Acarology Escola Superior de Agricultura Luiz de Queiroz (ESALQ) University of São Paulo Sugarcane Research Centre Instituto Agronômico de Campinas—IAC Crop Protection Research Centre Instituto Biológico—IB School of Agricultural and Veterinarian Sciences—FCAV São Paulo State University—UNESP CAPES: 001 CNPq: 314181/2020‐2

Made available in DSpace on 2021-06-25T11:16:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Agence Nationale Des Parcs Nationaux Centre for International Forestry Research Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS) David and Lucile Packard Foundation European Space Agency Leverhulme Trust Gordon and Betty Moore Foundation Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de Goiás European Research Council Belgian Federal Science Policy Office Vlaamse Interuniversitaire Raad Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) National Science Foundation Natural Environment Research Council Royal Society National Geographic Society Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Tropical forests are the most diverse and productive ecosystems on Earth. While better understanding of these forests is critical for our collective future, until quite recently efforts to measure and monitor them have been largely disconnected. Networking is essential to discover the answers to questions that transcend borders and the horizons of funding agencies. Here we show how a global community is responding to the challenges of tropical ecosystem research with diverse teams measuring forests tree-by-tree in thousands of long-term plots. We review the major scientific discoveries of this work and show how this process is changing tropical forest science. Our core approach involves linking long-term grassroots initiatives with standardized protocols and data management to generate robust scaled-up results. By connecting tropical researchers and elevating their status, our Social Research Network model recognises the key role of the data originator in scientific discovery. Conceived in 1999 with RAINFOR (South America), our permanent plot networks have been adapted to Africa (AfriTRON) and Southeast Asia (T-FORCES) and widely emulated worldwide. Now these multiple initiatives are integrated via ForestPlots.net cyber-infrastructure, linking colleagues from 54 countries across 24 plot networks. Collectively these are transforming understanding of tropical forests and their biospheric role. Together we have discovered how, where and why forest carbon and biodiversity are responding to climate change, and how they feedback on it. This long-term pan-tropical collaboration has revealed a large long-term carbon sink and its trends, as well as making clear which drivers are most important, which forest processes are affected, where they are changing, what the lags are, and the likely future responses of tropical forests as the climate continues to change. By leveraging a remarkably old technology, plot networks are sparking a very modern revolution in tropical forest science. In the future, humanity can benefit greatly by nurturing the grassroots communities now collectively capable of generating unique, long-term understanding of Earth's most precious forests. Resumen: Los bosques tropicales son los ecosistemas más diversos y productivos del mundo y entender su funcionamiento es crítico para nuestro futuro colectivo. Sin embargo, hasta hace muy poco, los esfuerzos para medirlos y monitorearlos han estado muy desconectados. El trabajo en redes es esencial para descubrir las respuestas a preguntas que trascienden las fronteras y los plazos de las agencias de financiamiento. Aquí mostramos cómo una comunidad global está respondiendo a los desafíos de la investigación en ecosistemas tropicales a través de diversos equipos realizando mediciones árbol por árbol en miles de parcelas permanentes de largo plazo. Revisamos los descubrimientos más importantes de este trabajo y discutimos cómo este proceso está cambiando la ciencia relacionada a los bosques tropicales. El enfoque central de nuestro esfuerzo implica la conexión de iniciativas locales de largo plazo con protocolos estandarizados y manejo de datos para producir resultados que se puedan trasladar a múltiples escalas. Conectando investigadores tropicales, elevando su posición y estatus, nuestro modelo de Red Social de Investigación reconoce el rol fundamental que tienen, para el descubrimiento científico, quienes generan o producen los datos. Concebida en 1999 con RAINFOR (Suramérica), nuestras redes de parcelas permanentes han sido adaptadas en África (AfriTRON) y el sureste asiático (T-FORCES) y ampliamente replicadas en el mundo. Actualmente todas estas iniciativas están integradas a través de la ciber-infraestructura de ForestPlots.net, conectando colegas de 54 países en 24 redes diferentes de parcelas. Colectivamente, estas redes están transformando nuestro conocimiento sobre los bosques tropicales y el rol de éstos en la biósfera. Juntos hemos descubierto cómo, dónde y porqué el carbono y la biodiversidad de los bosques tropicales está respondiendo al cambio climático y cómo se retroalimentan. Esta colaboración pan-tropical de largo plazo ha expuesto un gran sumidero de carbono y sus tendencias, mostrando claramente cuáles son los factores más importantes, qué procesos se ven afectados, dónde ocurren los cambios, los tiempos de reacción y las probables respuestas futuras mientras el clima continúa cambiando. Apalancando lo que realmente es una tecnología antigua, las redes de parcelas están generando una verdadera y moderna revolución en la ciencia tropical. En el futuro, la humanidad puede beneficiarse enormemente si se nutren y cultivan comunidades de investigadores de base, actualmente con la capacidad de generar información única y de largo plazo para entender los que probablemente son los bosques más preciados de la tierra. Resumo: Florestas tropicais são os ecossistemas mais diversos e produtivos da Terra. Embora uma boa compreensão destas florestas seja crucial para o nosso futuro coletivo, até muito recentemente os esforços de medições e monitoramento foram amplamente desconexos. É essencial formarmos redes para obtermos respostas que transcendem fronteiras e horizontes de agências financiadoras. Neste estudo nós mostramos como uma comunidade global está respondendo aos desafios da pesquisa de ecossistemas tropicais, com equipes diversas medindo florestas, árvore por árvore, em milhares de parcelas monitoradas à longo prazo. Nós revisamos as maiores descobertas científicas deste trabalho, e mostramos também como este processo está mudando a ciência de florestas tropicais. Nossa abordagem principal envolve unir iniciativas de base a protocolos padronizados e gerenciamento de dados a fim de gerar resultados robustos em escalas ampliadas. Ao conectar pesquisadores tropicais e elevar seus status, nosso modelo de Rede de Pesquisa Social reconhece o papel-chave do produtor dos dados na descoberta científica. Concebida em 1999 com o RAINFOR (América do Sul), nossa rede de parcelas permanentes foi adaptada para África (AfriTRON) e Sudeste asiático (T-FORCES), e tem sido extensamente reproduzida em todo o mundo. Agora estas múltiplas iniciativas estão integradas através de uma infraestrutura cibernética do ForestPlots.net, conectando colegas de 54 países de 24 redes de parcelas. Estas iniciativas estão transformando coletivamente o entendimento das florestas tropicais e seus papéis na biosfera. Juntos nós descobrimos como, onde e por que o carbono e a biodiversidade da floresta estão respondendo às mudanças climáticas, e seus efeitos de retroalimentação. Esta duradoura colaboração pantropical revelou um grande sumidouro de carbono persistente e suas tendências, assim como tem evidenciado quais direcionadores são mais importantes, quais processos florestais são mais afetados, onde eles estão mudando, seus atrasos no tempo de resposta, e as prováveis respostas das florestas tropicais conforme o clima continua a mudar. Dessa forma, aproveitando uma notável tecnologia antiga, redes de parcelas acendem faíscas de uma moderna revolução na ciência das florestas tropicais. No futuro a humanidade pode se beneficiar incentivando estas comunidades basais que agora são coletivamente capazes de gerar conhecimentos únicos e duradouros sobre as florestas mais preciosas da Terra. Résume: Les forêts tropicales sont les écosystèmes les plus diversifiés et les plus productifs de la planète. Si une meilleure compréhension de ces forêts est essentielle pour notre avenir collectif, jusqu'à tout récemment, les efforts déployés pour les mesurer et les surveiller ont été largement déconnectés. La mise en réseau est essentielle pour découvrir les réponses à des questions qui dépassent les frontières et les horizons des organismes de financement. Nous montrons ici comment une communauté mondiale relève les défis de la recherche sur les écosystèmes tropicaux avec diverses équipes qui mesurent les forêts arbre après arbre dans de milliers de parcelles permanentes. Nous passons en revue les principales découvertes scientifiques de ces travaux et montrons comment ce processus modifie la science des forêts tropicales. Notre approche principale consiste à relier les initiatives de base à long terme à des protocoles standardisés et une gestion de données afin de générer des résultats solides à grande échelle. En reliant les chercheurs tropicaux et en élevant leur statut, notre modèle de réseau de recherche sociale reconnaît le rôle clé de l'auteur des données dans la découverte scientifique. Conçus en 1999 avec RAINFOR (Amérique du Sud), nos réseaux de parcelles permanentes ont été adaptés à l'Afrique (AfriTRON) et à l'Asie du Sud-Est (T-FORCES) et largement imités dans le monde entier. Ces multiples initiatives sont désormais intégrées via l'infrastructure ForestPlots.net, qui relie des collègues de 54 pays à travers 24 réseaux de parcelles. Ensemble, elles transforment la compréhension des forêts tropicales et de leur rôle biosphérique. Ensemble, nous avons découvert comment, où et pourquoi le carbone forestier et la biodiversité réagissent au changement climatique, et comment ils y réagissent. Cette collaboration pan-tropicale à long terme a révélé un important puits de carbone à long terme et ses tendances, tout en mettant en évidence les facteurs les plus importants, les processus forestiers qui sont affectés, les endroits où ils changent, les décalages et les réactions futures probables des forêts tropicales à mesure que le climat continue de changer. En tirant parti d'une technologie remarquablement ancienne, les réseaux de parcelles déclenchent une révolution très moderne dans la science des forêts tropicales. À l'avenir, l'humanité pourra grandement bénéficier du soutien des communautés de base qui sont maintenant collectivement capables de générer une compréhension unique et à long terme des forêts les plus précieuses de la Terre. Abstrak: Hutan tropika adalah di antara ekosistem yang paling produktif dan mempunyai kepelbagaian biodiversiti yang tinggi di seluruh dunia. Walaupun pemahaman mengenai hutan tropika amat penting untuk masa depan kita, usaha-usaha untuk mengkaji dan mengawas hutah-hutan tersebut baru sekarang menjadi lebih diperhubungkan. Perangkaian adalah sangat penting untuk mencari jawapan kepada soalan-soalan yang menjangkaui sempadan dan batasan agensi pendanaan. Di sini kami menunjukkan bagaimana sebuah komuniti global bertindak balas terhadap cabaran penyelidikan ekosistem tropika melalui penglibatan pelbagai kumpulan yang mengukur hutan secara pokok demi pokok dalam beribu-ribu plot jangka panjang. Kami meninjau semula penemuan saintifik utama daripada kerja ini dan menunjukkan bagaimana proses ini sedang mengubah bidang sains hutan tropika. Teras pendekatan kami memberi tumpuan terhadap penghubungan inisiatif akar umbi jangka panjang dengan protokol standar serta pengurusan data untuk mendapatkan hasil skala besar yang kukuh. Dengan menghubungkan penyelidik-penyelidik tropika dan meningkatkan status mereka, model Rangkaian Penyelidikan Sosial kami mengiktiraf kepentingan peranan pengasas data dalam penemuan saintifik. Bermula dengan pengasasan RAINFOR (Amerika Selatan) pada tahun 1999, rangkaian-rangkaian plot kekal kami kemudian disesuaikan untuk Afrika (AfriTRON) dan Asia Tenggara (T-FORCES) dan selanjutnya telah banyak dicontohi di seluruh dunia. Kini, inisiatif-inisiatif tersebut disepadukan melalui infrastruktur siber ForestPlots.net yang menghubungkan rakan sekerja dari 54 negara di 24 buah rangkaian plot. Secara kolektif, rangkaian ini sedang mengubah pemahaman tentang hutan tropika dan peranannya dalam biosfera. Kami telah bekerjasama untuk menemukan bagaimana, di mana dan mengapa karbon serta biodiversiti hutan bertindak balas terhadap perubahan iklim dan juga bagaimana mereka saling bermaklum balas. Kolaborasi pan-tropika jangka panjang ini telah mendedahkan sebuah sinki karbon jangka panjang serta arah alirannya dan juga menjelaskan pemandu-pemandu perubahan yang terpenting, di mana dan bagaimana proses hutan terjejas, masa susul yang ada dan kemungkinan tindakbalas hutan tropika pada perubahan iklim secara berterusan di masa depan. Dengan memanfaatkan pendekatan lama, rangkaian plot sedang menyalakan revolusi yang amat moden dalam sains hutan tropika. Pada masa akan datang, manusia sejagat akan banyak mendapat manfaat jika memupuk komuniti-komuniti akar umbi yang kini berkemampuan secara kolektif menghasilkan pemahaman unik dan jangka panjang mengenai hutan-hutan yang paling berharga di dunia. Instituto de Ecología Regional (IER) Universidad Nacional de Tucumán (UNT) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Facultad de Ciencias Agrarias Universidad Nacional de Jujuy James Cook University (JCU) CSIRO (Commonwealth Scientific and Industrial Research Organisation) School of Land & Food University of Tasmania CSIRO Tropical Forest Research Centre Independent Researcher Environmental Protection Agency (EPA) Centre for Tropical Environmental and Sustainability Science (TESS) College of Marine and Environmental Sciences James Cook University Centre for Tropical Environmental and Sustainability Science College of Science and Engineering James Cook University University of the Sunshine Coast University of York Flamingo Land Ltd. Sommersbergseestrasse Ghent University CAVElab Ghent University Royal Museum for Central Africa - Service of Wood Biology Isotope Bioscience Laboratory-ISOFYS Ghent University Gembloux Agro-Bio Tech Université de Liege Landscape Ecology and Vegetal Production Systems Unit CAVElab Computational & Applied Vegetation Ecology Ghent University Tropical Forestry Forest Resources Management Gembloux Agro-Bio Tech University of Liege Université Libre de Bruxelles (ULB) Evolutionary Biology and Ecology Royal Museum for Central Africa Royal Museum for Central Africa Ghent University Department of Environment Ghent University Service Evolution Biologique et Ecologie Belize Foundation for Research and Environmental Education IBIF Museo de Historia Natural Noel Kempff Mercado Universidad Autonoma Gabriel Rene Moreno PROMAB Museo Noel Kempff Consultor Independiente Jardin Botanico Municipal de Santa Cruz Museo de Historia Natural Noel Kempff Mercado Forest Management in Bolivia Universidad Autónoma del Beni Riberalta Museo de Historia Natural Noel Kempff Herbario del Sur de Bolivia Universidad Autónoma del Beni Conservation International Instituto de Biodiversidade e Floresta Universidade Federal do Oeste do Pará Universidade Federal de Pernambuco Universidade do Estado de Mato Grosso Universidade do Estado de Mato Grosso (UNEMAT) Projeto TEAM – Manaus Universidade Federal de Juiz de Fora (UFJF) Universidade Federal do Rio de Janeiro Instituto Nacional de Pesquisas da Amazônia Projeto Dinâmica Biológica de Fragmentos Florestais Departamento de Genética Ecologia e Evolução Universidade Federal de Minas Gerais Universidade Estadual de Campinas Laboratório de Ecologia de Comunidades e Funcionamento de Ecossistemas-ECoFERP Departamento de Biologia Faculdade de Filosofia Ciências e Letras USP National Institute for Space Research (INPE) Universidade Federal de Roraima (UFRR) UNESP - São Paulo State University Carbonozero Consultoria Ambiental Departamento de Biologia Universidade Federal do Amazonas (UFAM) Centro de Energia Nuclear na Agricultura Universidade de São Paulo UERR - Campus Rorainópolis Universidade Federal do Acre Instituto Agronômico de Campinas Universidade Federal do Rio Grande do Sul Embrapa Universidade Estadual do Norte Fluminense (UENF) Universidade Federal da Bahia (UFBA) Instituto de Biologia Universidade Estadual de Campinas Universidade Federal do Piauí (UFPI) Botany and Plant Ecology Laboratory Federal University of Acre INPA- Instituto Nacional de Pesquisas da Amazônia UERR - Campus Boa Vista Universidade Federal do Ceará Universidade Federal de Campina Grande Universidade Federal do Para Núcleo de Estudos e Pesquisas Ambientais Universidade Estadual de Campinas Instituto Federal de Educação Ciência e Tecnologia do Acre Instituto de Pesquisas Jardim Botânico do Rio de Janeiro Universidade Federal do Oeste do Pará UEFS Depto. de Ciências Biológicas Universidade Federal do Agreste de Pernambuco (UFAPE) Universidade Estadual de Montes Claros FFCLRP-USP/Br UNEMAT Universidade Federal de Jataí Universidade Federal do Pará Instituto de Ciências Biológicas Universidade de Campinas Universidade Federal de Lavras (UFLA) Museu Goeldi Embrapa Amazônia Oriental Instituto Nacional de Pesquisas da Amazônia UFMG - Universidade Federal de Minas Gerais Fundação Universidade Fedral de Rondônia - UNIR INPA- Instituto Nacional de Pesquisas Amazônicas Instituto Nacional de Pesquisas da Amazônia - Coordenação de Pesquisas em Silvicultura Tropical Jardim Botânico do Rio de Janeiro National Institute for Research in Amazonia Universidade Federal de Roraima (UFRR/PRONAT) Universidade Estadual de Campinas/UNICAMP Instituto Nacional de Pesquisas da Amazônia/CPBO Universidade Federal de Santa Catarina (UFSC) INCAPER- Instituto Capixaba de Pesquisa Assistência Técnica e Extensão Rural INPE- Instituto Nacional de Pesquisas Espaciais Universidade de São Paulo Instituto de Biociências Universidade Estadual Paulista Semiarid National Institute (INSA) Universidade de Brasília Departamento de Engenharia Florestal IBAM - Instituto Bem Ambiental Universidade do Estado de Mato Grosso Campus de Nova Xavantina University in Campinas Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) LMF Instituto Nacional de Pesquisas da Amazônia Universidade Federal do Vale do São Francisco USP - University of São Paulo Instituto Federal do Espírito Santo (IFES) INPA - Instituto Nacional de Pesquisas da Amazônia Grupo MAUA Universidade Federal de Mato Grosso Instituto de Ciências Naturais Humanas e Sociais Instituto Nacional da Mata Atlântica RAINFOR-PPBIO Universidade Federal Rural da Amazônia - UFRA/CAPES Universidade Federal do Amazonas (UFAM) INPA/Max-Planck Project EMBRAPA- Empresa Brasileira de Pesquisa Agropecuária (Amazônia Oriental) Serviço Florestal Brasileiro Museu Universitário Universidade Federal do Acre Universidade Federal Rural de Pernambuco PUCPR - Pontifícia Universidade Católica do Paraná Museu Paraense Emilio Goeldi Universiti Brunei Darussalam Environmental and Life Sciences Faculty of Science Universiti Brunei Darussalam Institute for Biodiversity and Environmental Research Universiti Brunei Darussalam Plant Systematic and Ecology Laboratory Department of Biology Higher Teachers’ Training College University of Yaounde I Faculty of Science Department of Botany and Plant Physiology University of Buea Faculty of Science Department of Plant Science University of Buea National Herbarium Plant Systematics and Ecology Laboratory Higher Teachers’ Training College University of Yaoundé I Department of Plant Biology Faculty of Sciences University of Yaounde 1 Bioversity International Faculty of Forestry University of Toronto Ministère des Eaux Forêts Chasse et Pêche (MEFCP) Universidad Católica de la Santísima Concepción Universidad de La Serena Research Institute of Tropical Forestry Chinese Academy of Forestry Kunming Institute of Botany Chinese Academy of Sciences Beijing Forestry University Universidad Nacional Abierta y a Distancia Red COL-TREE Corporación COL-TREE Nuevo Estándar Biotropical NEBIOT SAS Universidad del Tolima Asociación GAICA Universidad de Nariño – Red BST-Col Parques Nacionales Naturales Territorial Caribe – Red BST-Col Universidad del Atlantico – Red BST-Col Departamento de Ciencias Forestales Universidad Nacional de Colombia - Sede Medellín Socioecosistemas y Clima Sostenible Fundacion con Vida Parques Nacionales Naturales de Colombia – Red BST-Col Instituto de Investigación de Recursos Biológicos Alexander von Humboldt – Red BST-Col UNAL Instituto de Investigación Recursos Biologicos Alexander von Humboldt – Red BST-Col Fundación Jardín Botánico de Medellín Herbario “Joaquín Antonio Uribe” (JAUM) – Red BST-Col Universidad Nacional de Colombia sede Amazonia Coltree Facultad del Medio Ambiente y Recursos Naturales Universidad Distrital Francisco José de Caldas – Red BST-Col Universidad de Tolima Fundación Orinoquia Biodiversa – Red BST-Col Departamento de Biología Facultad de Ciencias Naturales Universidad Icesi – Red BST-Col Instituto de Ciencias Naturales Universidad Nacional de Colombia Universidad de los Llanos Servicios Ecoysistemicos y Cambio Climatico (SECC) Fundación Con Vida & Corporación COL-TREE Universidad del Rosario Fundacion Ecosistemas Secos de Colombia – Red BST-Col Universidad de los Andes - ANDES herbarium Institute of Botany Czech Academy of Sciences Palacky University Czech University of Life Sciences Mendel University World Wide Fund for Nature Wildlife Conservation Society-DR Congo Lukuru Wildlife Research Foundation Université de Kisangani Faculté des Sciences Laboratoire d'écologie et aménagement forestier Université de Kisangani Université de Kisangani Faculté des Sciences Agronomiques République Démocratique du Congo Ministère de l'Environnement et Développement Durable Aarhus University University of Copenhagen Escuela Politécnica Nacional del Ecuador Herbario Alfredo Paredes (QAP) Universidad Central del Ecuador Universidad Técnica Particular de Loja Grupo de Investigación en Biodiversidad Medio Ambiente y Salud-BIOMAS Universidad de las Américas, Campus Queri Keller Science Action Center The Field Museum, 1400 South Lake Shore Dr. Universidad Estatal Amazónica Facultad de Ingeniería Ambiental Universidad Tecnica del Norte Herbario Nacional del Ecuador Grupo de Ecosistemas Tropicales y Cambio Global Universidad Regional Amazónica ikiam Colegio de Ciencias Biológicas y Ambientales COCIBA & Extensión Galápagos Universidad San Francisco de Quito-USFQ Herbario de Botánica Económica del Ecuador QUSF Universidad San Francisco de Quito USFQ Galapagos Science Center USFQ UNC Chapel Hill University of North Carolina-UNC Chapel Hill University of Florida FindingSpecies Mekelle University Environment Climate Change and Coffee Forest Forum (ECCCFF) University of Turku Centre de coopération International en Recherche Agronomique pour le Développement (CIRAD) CNRS ONF INRAE Centre National de la Recherche Scientifique AMAP Univ Montpellier IRD CNRS CIRAD INRA Forêts et Sociétés (F&S) Centre de coopération International en Recherche Agronomique pour le Développement (CIRAD) Departement Hommes Natures Societes Museum national d'histoire naturelle INRA Cirad UMR Ecologie des Forêts de Guyane (AgroparisTech CNRS INRAE Université des Antilles Université de la Guyane) Ministry of Forests Seas Environment and Climate Rougier-Gabon Agence Nationale des Parcs Nationaux Gabon Commission of Central African Forests (COMIFAC) Agence Nationale des Parcs Nationaux Ministère des Forêts des Eaux de la Mer de l'Environnement Chargé du Plan Climat des Objectifs de Développement Durable et du Plan d'Affectation des Terres Institut de Recherche en Ecologie Tropicale (CENAREST) Gabon/Agence Nationale des Parcs Nationaux Georg-August-University Göttingen University of Freiburg Institute of Botany University of Hohenheim Max Planck Institute for Chemistry Forestry Research Institute of Ghana (FORIG) Mensuration Unit Forestry Commission of Ghana Center for International Forestry Research Iwokrama International Centre for Rainforest Conservation and Development Guyana Forestry Commission Utrecht University Centre for Sustainable Technologies Indian Institute of Science Centre for International Forestry Research (CIFOR) Herbarium Borgoriense Indonesian Institute of Sciences (LIPI) Indonesian Institute of Science Forest Research and Development Agency (FORDA) Balitek-KSDA Samboja University of Florence and MUSE - Museo delle Scienze Cirad Hokkaido University Graduate School of Agriculture Kyoto University Forestry Development Authority of the Government of Liberia (FDA) University of Liberia Sungai Wain Protection Forest South East Asia Rainforest Research Partnership Danum Valley Field Centre Malaysian Palm Oil Board Sabah Forestry Department Forest Research Centre Universiti Malaysia Sabah Sabah Forestry Department Sarawak Forestry Corporation Eduardo Mondlane University Herbarium UNAN-Leon Universidad Nacional Autónoma de Nicaragua University of Abeokuta Natural History Museum of Norway University of Oslo Norwegian University of Life Sciences Universidad Nacional de la Amazonía Peruana (UNAP) Universidad Nacional de Jaén Jardin Botanico de Missouri Andes to Amazon Biodiversity Program Universidad Nacional de San Agustín de Arequipa Facultad de Ciencias Biológicas Universidad Nacional de la Amazonía Peruana Kené - Instituto de Estudios Forestales y Ambientales Instituto de Investigaciones de la Amazonia Peruana (IIAP) Universidad Nacional Jorge Basadre de Grohmann (UNJBG) Universidad Nacional de San Antonio Abad del Cusco Centro de Conservación Investigación y Manejo CIMA Pontificia Universidad Católica del Perú Asociacion Bosques Perú Université Officielle de Bukavu Université Marien N'Gouabi Wildlife Conservation Society Ecole Nationale Supérieure d'Agronomie et de Foresterie Université Marien Ngouabi Univeriste Marien Ngouabi The Gola Rainforest National Park Department of Geography National University of Singapore Departamento de Biología y Geología Física y Química inorgánica Universidad Rey Juan Carlos Real Jardín Botánico – CSIC Departamento de Biología Área de Botánica Universidad Autónoma de Madrid Museo Nacional de Ciencias Naturales (MNCN-CSIC) Centre for Agricultural Research in Suriname (CELOS) Stockholm Environment Institute Department of Plant Ecology and Evolution Uppsala University Southern Swedish Forest Research Centre InfoFlora Conservatoire et Jardin Botanique Geneve National Chung Hsing University Sokoine University of Agriculture Naturalis Biodiversity Center Wageningen University Forest Ecology and Forest Management Group Tropenbos International Institute for Biodiversity and Ecosystem Dynamics University of Amsterdam Bureau Waardenburg BV Van Hall Larenstein University of Applied Sciences Van der Hout Forestry Consulting Utrecht University, Domplein 29 Wageningen University Plant Ecology and Nature Conservation Group Data for Sustainability Department of Zoology Entomology & Fisheries Sciences Makerere University The Institute of Tropical Forest Conservation (ITFC) Mbarara University of Science and Technology (MUST) George Washington University Makerere University Department of Forestry Biodiversity and Tourism Makerere University University of Stirling University of Kent School of Geography University of Leeds UK Centre of Ecology & Hydrology Lancaster University University of Oxford The Landscapes and Livelihoods Group (TLLG) Overseas Development Institute Manchester Metropolitan University University of Aberdeen University of Exeter School of GeoSciences University of Edinburgh University of Cambridge Department of Environment and Geography University of York Department of Geography University College London Imperial College School of Geography Earth & Environmental Sciences Birmingham Institute of Forest Research University of Birmingham University of Plymouth Geography College of Life and Environmental Sciences University of Exeter Lancaster Environment Centre Lancaster University University of Edinburgh School of Biology University of Leeds Royal Botanic Garden Edinburgh CENAREST & ANPN & Stirling University University of Bristol School of Biological Sciences Department of Plant Sciences University of Cambridge Living with Lake Centre Laurentian University Royal Botanic Gardens Kew The Royal Society for the Protection of Birds centre for Conservation Science Environmental Change Institute School of Geography and the Environment University of Oxford UK Centre for Ecology & Hydrology School of Geography and the Environment University of Oxford The Royal Botanic Gardens Department of Geography and Environmental Science University of Dundee School of Biological Sciences University of Southampton University of East Anglia Stirling University School of Earth and Environment University of Leeds Department of Plant & Soil Science School of Biological Sciences University of Aberdeen, Cruickshank Building Institute for Transport Studies University of Leeds UK Research & Innovation University of Nottingham University of Bangor Center for Tropical Research Institute of the Environment and Sustainability University of California Center for Tropical Conservation Nicholas School of the Environment Duke University Ecology and Evolutionary Biology University of Michigan Saint Louis Zoo Department of Organismic and Evolutionary Biology Harvard University Center for Global Discovery and Conservation Science Arizona State University Wildlife Conservation Society – Programme Congo Woods Hole Research Center The University of Michigan Herbarium Nicholas School of the Environment National Park Service University of California ForestGEO Smithsonian Tropical Research Institute University of Texas at Austin Smithsonian Institute Washington University in Saint Louis Center for Conservation and Sustainable Development at the Missouri Botanical Garden Smithsonian Tropical Research Institute Smithsonian Institution Forest Global Earth Observatory (ForestGEO) Forest Global Earth Observatory (ForestGEO) Smithsonian Tropical Research Institute George Mason University Missouri Botanical Garden Broward County Parks and Recreation Nova Southeastern University Science and Education The Field Museum Department of Biology Boston University Wake Forest University Department of Geographical Sciences University of Maryland San Diego Zoo Institute for Conservation Research Biology Department Washington State University Yale School of Forestry & Environmental Studies Columbia University Department of Environmental Science Policy and Management University of California Berkeley School of Earth Sciences and Environmental Sustainability Northern Arizona University Department of Geography and the Environment University of Texas at Austin UNELLEZ-Guanare Programa de Ciencias del Agro y el Mar Herbario Universitario (PORT) Ci Progress GreenLife Universidad Nacional Experimental de Guayana Instituto Venezolano de Investigaciones Científicas (IVIC) Universidad de los Andes Viet Nature Conservation Centre CIRAD School of Life Sciences University of Lincoln UNESP - São Paulo State University Instituto de Biociências Universidade Estadual Paulista Gordon and Betty Moore Foundation: 1656 FAPESP: 2012/51509-8 FAPESP: 2012/51872-5 Fundação de Amparo à Pesquisa do Estado de Goiás: 2017/10267000329 European Research Council: 291585 Gordon and Betty Moore Foundation: 5349 European Research Council: 758873 Belgian Federal Science Policy Office: BR/132/A1/AFRIFORD Belgian Federal Science Policy Office: BR/143/A3/HERBAXYLAREDD Vlaamse Interuniversitaire Raad: CD2018TEA459A103 CNPq: CNPq/PPBio/457602/2012-0 National Science Foundation: DEB 1754647 Natural Environment Research Council: E/M0022021/1 Royal Society: ICA/R1/180100 Natural Environment Research Council: NE/D005590/1 European Research Council: NE/F005806/1 Natural Environment Research Council: NE/F005806/1 FAPESP: NE/K016431/1 Natural Environment Research Council: NE/N004655/1 FAPESP: NE/N012542/1 Royal Society: NE/P008755/1 FAPESP: NE/S011811/1 National Geographic Society: NE/T01279X/1 CNPq: PELD/441244/2016-5 Belgian Federal Science Policy Office: SD/AR/01A/COBIMFO

The concept of precision conservation can be defined as a set of space technologies and other procedures linked to mappable environmental variables, which can be used to program conservation management practices for natural resources that consider the variability of these variables in space and time within of natural or agricultural systems. In this context, structural loss of soil through human activities is considered, as with a process with a spatial and temporal variation. The management of soil aggregation conditions can contribute to more regenerative and sustainable agricultural processes. It allows spatial analysis technologies through georeferenced visual indicators or even the use of systems with automatic learning, known as deep learning. In this sense, a fair visual method was developed with an analysis of fuzzy logic to classify aggregates in terms of shape, surface roughness, and biogenic structures. Thus, in a second stage, a model of the artificial neural network was developed, capable of detecting and classifying different forms of soil aggregates, thus allowing a brief discussion of the theme and its potential for application in conservation management through the analysis of aggregates via systems automatic sorting. In this way, elements are presented for the motivation of research and development in adaptive technologies in supporting decision-making that can help integrate dynamic and spatial information in the understanding of the soil’s structural condition to preserve the soil more precisely. RESUMO O conceito de conservação de precisão pode ser definido como um conjunto de tecnologias espaciais e outros procedimentos ligados as variáveis ambientais mapeáveis, que podem ser utilizadas para programar práticas de gestão da conservação recursos naturais que levam em consideração a variabilidade dessas variáveis no espaço e tempo dentro de sistemas naturais ou agrícolas. Nesse contexto, considera-se a perda estrutural do solo por meio de atividades antrópicas, como com um processo com variação espacial e temporal. A gestão da condição de agregação do solo pode contribuir para processos agrícolas mais regenerativos e sustentáveis, pois permite a utilização das tecnologias de análise espacial, por meio de indicadores visuais georreferenciados ou mesmo a utilização de sistemas com aprendizado automático, conhecidos como deep learning. Nesse sentido, foi desenvolvido um método visual justaposto com uma análise de lógica fuzzy para a classificação dos agregados quanto à forma, rugosidade superficial e estruturas biogênicas. Assim, numa segunda etapa foi desenvolvido um modelo de rede neural artificial capaz de detectar e classificar diferentes formas de agregados do solo, permitindo dessa maneira, uma breve discussão da temática e seu potencial de aplicação na gestão conservacionista por meio da análise de agregados via sistemas automáticos de classificação. Dessa maneira, são apresentados elementos para a motivação de pesquisas e desenvolvimento em tecnologias adaptativas, no apoio à decisão que possam auxiliar a integração de informações dinâmicas e espaciais no entendimento da condição estrutural do solo com a finalidade de uma conservação dos solos com mais precisão.

Made available in DSpace on 2022-04-28T19:28:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-01 Huanglongbing (HLB) is a severe disease of citrus caused by the bacterium Candidatus Liberibacter. In America, the most common species is Candidatus Liberibacter asiaticus (CLas). In a previous study of the Citrus-HLB pathosystem, our group found differences in CLas multiplication in a population of hybrids obtained by hybridization between Citrus sunki Hort. Ex Tan. and Poncirus trifoliata (L.) Raf. cv. Rubidoux. The bacterial concentration was higher in C. sunki than in P. trifoliata. Thus, this study aims to map phenotypic (QTL) and gene expression (eQTL) data associated with host response to CLas in the linkage groups (LGs) of the previously constructed parental maps of C. sunki and P. trifoliata cv. Rubidoux. For the phenotypic analysis, we used a population of 79 F1 hybrids (termed citrandarins) between C. sunki and P. trifoliata. CLas and starch were quantified in the leaves of the plants two years after pathogen inoculation, allowing the classification of hybrids as resistant, tolerant, and susceptible. The expression of 14 candidate genes was measured in 72 hybrids of the population and used as expression data for the eQTL mapping. We located nine QTL and 52 eQTL in the C. sunki map and 17 QTL and 40 eQTL in the P. trifoliata map. The overlap of eQTL of the majority of genes with QTL from the phenotypic data indicates that the genes are related to the phenotype and are probably related to pathogen infection. Centro APTA Citros Sylvio Moreira Instituto Agronômico de Campinas Laboratório de Bioinformática Centro de Ciências Biológicas Universidade Federal de Santa Catarina Universidade Estadual de Campinas Hill Laboratories Departamento de Genética Instituto de Biociências Universidade do Estado de São Paulo Centro de Ciências Agrárias Universidade Federal de São Carlos

Made available in DSpace on 2021-06-25T11:11:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01 The brown seaweed Ascophyllum nodosum and seaweed-derived products have been widely used as nutrient additions, biofertilizer and biostimulant in horticultural crop systems. This study aimed to evaluate the effects of a seaweed extract obtained from whole brown algae (A nodosum) on seed germination and seedling growth of ornamental marigold (Tagetes erecta). The seeds of marigold were daily sprayed with 0 (control), 5, 10 or 15 mL L−1 commercial seaweed extract Acadian Seaplants™ (70 ml). The percentage, index and average time of seed germination, seedling height, fresh, dry mass of shoots, roots and root system morphology were evaluated. The results showed that the seaweed extract enhanced all parameters related to seed germination and seedling growth and development. The best results were obtained with 15 mL L−1 seaweed extract which increased by two-fold marigold seed germination and 84.00% seedling height, and both fresh and dry shoot mass and root morphology. We suggest algal extracts of Ascophyllum nodosum should be used as natural biostimulant on marigold crop, improving seed germination and seedling development. Instituto Agronômico de Campinas (IAC) Universidade Estadual Paulista (UNESP) Universidade Estadual Paulista (UNESP)

Made available in DSpace on 2020-12-12T02:50:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-03 Intercropping forage grasses with upland rice is an alternative cropping system to improve agroecosystem diversification and could potentially enhance sustainability in tropical regions. However, nitrogen (N) immobilization and nutrient competition between rice and forage grasses could reduce rice grain yield and decrease overall productivity. Therefore, fertilizer N requirements of upland rice intercropped with forage grasses needs to be better defined. Field experiments were carried out during three growing seasons on a Typic Haplorthox soil in São Paulo state of Brazil. The experimental design was a randomized block design with a 3 × 4 factorial scheme with four replications. Treatments were cropping system [monocropped rice (Oryza sativa L.), rice intercropped with palisadegrass (Urochloa brizantha), and rice intercropped with guineagrass (Megathyrsus maximus) and sidedress N application rate (0, 40, 80, and 120 kg N ha−1). Intercropped grasses were sown between upland rice rows 30 days after rice emergence. On average, intercropping of rice with palisadegrass or guineagrass decreased rice shoot dry matter and grain yield by 11% and milled rice productivity by 10% compared with monocropped rice. Grain yield, grain protein, and milled productivity of rice increased as N application rate increased. Forage dry matter production (first and second cut) and crude protein (second cut) were greatest in the rice + palisadegrass intercropping system. Production of both forage grasses increased with up to 80 kg N ha−1 in the first cut and increased linearly with N in the second cut. Intercropping of rice with palisadegrass or guineagrass with 80 kg N ha−1 application resulted in the greatest land equivalent ratio (1.96 and 1.55, respectively). Relative N yield was greatest at 120 kg N ha−1 (220 and 173%, respectively). Although rice monocropping had greatest grain yield, intercropping systems with forage grasses were more favorable from both economic and environmental perspectives by enhancing plant diversification, nutrient cycling with forage grasses, land use production per unit area, and profitability throughout the year. UNESP Department of Soil Science College of Agricultural Science NIOO-KNAW Department of Microbial Ecology Netherlands Institute of Ecology USDA Agricultural Research Service NCSU Campus IAC Soils and Environmental Resources Center Instituto Agronômico de Campinas UNESP Department of Soil Science College of Agricultural Science

Glyphosate is the main tool for weed management in Brazilian citrus orchards, where weeds, such as Conyza bonariensis and Digitaria insularis, have been found with resistance to this herbicide. Field prospections have allowed the identification of a possible new case of glyphosate resistance. In this work, the susceptibility levels to glyphosate on three Amaranthus viridis L. populations, with suspected resistance (R1, R2, and R-IAC), collected in citrus orchards from the S&atilde, o Paulo State, Brazil, as well as their accumulation rates of shikimic acid, were determined. The fresh weight of the susceptible population (S) was reduced by 50% (GR50) with ~30 g ea ha&minus, 1 glyphosate, while the GR50 values of the R populations were between 5.4 and 11.3 times higher than that for S population. The LD50 (herbicide dose to kill 50% of individuals of a weed population) values of the S population were &le, 150 g ea ha&minus, 1 glyphosate, while the LD50 of the R populations ranged from 600 to 920 g ea ha&minus, 1. Based on the reduction of fresh weight and the survival rate, the R1 population showed the highest level of glyphosate resistance, which had GR50 and LD50 values of 248 and 918 g ea ha&minus, 1 glyphosate, respectively. The S population accumulated 240 &micro, g shikimic acid at 1000 &micro, M glyphosate, while the R1, R2, and R-IAC populations accumulated only 16, 43, and 33 &micro, g shikimic acid, respectively (between 5.6 to 15 times less than the S population). Enzyme activity assays suggested that at least one target site-type mechanism was involved in resistance. This result revealed the first report of glyphosate resistance in A. viridis reported in the world.

Made available in DSpace on 2020-12-12T01:48:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) In tropical regions, one of the primary difficulties in the sustainability of no-till systems is the maintenance of soil cover throughout the year. Intercropping corn (Zea mays L.) with tropical perennial grasses is an innovative approach to overcome this difficulty. However, the use of sidedress nitrogen (N) fertilization in this intercropping system remains incompletely studied. The effects of using sidedress N application rates (0, 50, 100 and 200 kg ha−1 N) on monocropped corn or corn intercropped with palisadegrass [Urochloa brizantha (Hochst. ex A. Rich.) R. Webster ‘Marandu’] or guineagrass [Megathyrsus maximum (Jacq.) R. Webster ‘Mombaça’] with respect to leaf nutrient concentration, agronomic characteristics, revenue and land use efficiency were investigated over three growing seasons in the Brazilian Cerrado region on a clay soil, kaolinitic, thermic Typic Haplorthox. In general, the intercropping systems of corn with palisade or guineagrass did not cause reductions in the leaf nutrient concentration, agronomic characteristics, kernels attributes, shoot dry matter and corn yield compared to that observed in the monocropped system. High sidedress N application rates promoted higher concentrations of nutrients in leaves (primarily N and P), corn yield, forage production and crude protein concentration in the off-season. Corn monocropped or intercropped with palisadegrass and guineagrass using 113, 156 and 187 kg ha−1 N resulted in the highest corn yields (9.2, 8.8 and 9.7 Mg ha−1, respectively). In addition, using these N application rates, corn intercropped with palisadegrass and guineagrass and subsequent meat production estimated on pastures resulted in the highest net profits (US$ 1196 and 1093 ha−1, respectively), whereas monocropped corn without N addition resulted in the lowest net profits (US$ 304 ha−1). Thus, forage produced during the off-season can be used for animal grazing or fodder during the dry season, increasing revenue. Corn intercropped with palisade grass with the addition of 200 kg ha−1 N resulted in a higher land equivalent ratio and relative N yield (1.21 and 123%, respectively). In summary, sidedress N fertilization in intercropped systems can increase crop and forage yields and revenue, improving land-use efficiency in tropical agriculture. São Paulo Agency of Agribusiness Technology (APTA) Department of Crop Science College of Agricultural Science São Paulo State University (UNESP) Department of Animal Nutrition and Breeding School of Veterinary Medicine and Animal Science UNESP College of Agricultural Science UNESP Corn and Sorghum Research Center Brazilian Agricultural Research Corporation (EMBRAPA) USDA – Agricultural Research Service, 3218 Williams Hall, NCSU Campus, Box 7619 Soils and Environmental Resources Center Instituto Agronômico de Campinas (IAC) Department of Crop Science College of Agricultural Science São Paulo State University (UNESP) Department of Animal Nutrition and Breeding School of Veterinary Medicine and Animal Science UNESP College of Agricultural Science UNESP FAPESP: 2003/01968-7 FAPESP: 2003/09914-3

Made available in DSpace on 2020-12-12T02:45:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Sewage sludge is a residual pollutant product from the treatment of urban effluent and must be adequately processed before final disposal in order to avoid environmental contamination. The use of sewage sludge in agricultural and forestry areas can improve the physical, chemical and biological properties of soil; it can also be a source of nutrients, increasing crop productivity. Accordingly, this study aimed to evaluate the growth and development of hybrid Eucalyptus urograndis (Eucalyptus grandis x E. urophylla) seedlings cultivated in soil with different doses of sewage sludge. To accomplish this, hybrid E. urograndis seedlings were cultivated for 120 days in pots containing soil (dystrophic Red-Yellow Latosol-LVA) with different doses of sewage sludge (0, 30, 60 or 90 Mg ha-1). The experiment was carried out in a randomized complete block design consisting of four treatments, three replications with four plants per replication, totaling 48 plants. Biometric, biomass and physiological variables (chlorophyll, gaseous exchange, stomatal conductance and transpiration); in addition to macro-and micronutrient contents in leaves and soils of each treatment were evaluated. The treatments with sewage sludge were statistically superior to control treatment for all variables. Therefore, we recommend the application of 60 Mg ha-1 sewage sludge for the growth of hybrid Eucalyptus urograndis seedlings. Conversely, 90 Mg ha-1 sewage sludge inhibited hybrid E. urograndis root growth. Moreover, the use of sewage sludge as organic fertilizer in tropical soils is a viable and sustainable alternative for Eucalyptus cultivation. Instituto de Botânica, Av. Miguel Stefano Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Av. Universitária Instituto Agronômico de Campinas, Avenida Luiz Pereira dos Santos Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Av. Universitária

Made available in DSpace on 2020-12-12T01:24:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01. Added 1 bitstream(s) on 2021-07-15T15:25:29Z : No. of bitstreams: 1 S2179-80872020000200109.pdf: 787337 bytes, checksum: 065a265919690af8af0a98b462478740 (MD5) Tropical forests include remnants that should be characterized and monitored for long-term conservation. With this in mind, we performed a survey of the floristic composition and structure of the Mata Santa Elisa (Campinas, SP), and compared it to other fragments of semi-deciduous seasonal forest in the municipality. In Mata Santa Elisa, 506 living arboreal specimens were found, belonging to 100 species, 75 genera and 32 families. At the time of this work, the fragment was well-preserved and contained exclusive species and those of economic interest or potential, highlighting its importance in the region. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Instituto Agronômico de Campinas (IAC) Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)

International audience; Sugarcane (Saccharum spp.) is highly polyploid and aneuploid. Modern cultivars are derived from hybridization between S. officinarum and S. spontaneum. This combination results in a genome exhibiting variable ploidy among different loci, a huge genome size (similar to 10 Gb) and a high content of repetitive regions. An approach using genomic, transcriptomic, and genetic mapping can improve our knowledge of the behavior of genetics in sugarcane. The hypothetical HP600 and Centromere Protein C (CENP-C) genes from sugarcane were used to elucidate the allelic expression and genomic and genetic behaviors of this complex polyploid. The physically linked side-by-side genes HP600 and CENP-C were found in two different homeologous chromosome groups with ploidies of eight and ten. The first region (Region01) was a Sorghum bicolor ortholog region with all haplotypes of HP600 and CENP-C expressed, but HP600 exhibited an unbalanced haplotype expression. The second region (Region02) was a scrambled sugarcane sequence formed from different noncollinear genes containing partial duplications of HP600 and CENP-C (paralogs). This duplication resulted in a non-expressed HP600 pseudogene and a recombined fusion version of CENP-C and the orthologous gene Sobic. 003G299500 with at least two chimeric gene haplotypes expressed. It was also determined that it occurred before Saccharum genus formation and after the separation of sorghum and sugarcane. A linkage map was constructed using markers from nonduplicated Region01 and for the duplication (Region01 and Region02). We compare the physical and linkage maps, demonstrating the possibility of mapping markers located in duplicated regions with markers in nonduplicated region. Our results contribute directly to the improvement of linkage mapping in complex polyploids and improve the integration of physical and genetic data for sugarcane breeding programs. Thus, we describe the complexity involved in sugarcane genetics and genomics and allelic dynamics, which can be useful for understanding complex polyploid genomes.

Made available in DSpace on 2019-10-06T17:07:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-04-15 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Bemisia tabaci is an important agriculture pests and vector of viruses. The MEAM1 species of B. tabaci, first described in Brazil in the 90s is now the most prevalent species and primary cause of the emergence of begomoviruses in tomatoes. The Mediterranean species (MED) was recently detected in Brazil and is a new concern for Brazilian agriculture. The potential impact of this species as a vector of economically important virus in Brazil is unknown. We therefore evaluated the ability of MED to transmit four whitefly transmitted viruses prevalent in Brazil, Cowpea mild mottle virus (CpMMV, carlavirus), Bean golden mosaic virus (BGMV, begomovirus) infecting beans; and the Tomato severe rugose virus (ToSRV, begomovirus), Tomato chlorosis virus (ToCV, crinivirus) infecting tomatoes. The colony of MED harbouring the secondary endosymbionts was tested: 14% positive for Hamiltonella and 29% positive for Rickettsia. After six months being maintained on cotton plants, this colony changed the frequency of endosymbionts (97% of Hamiltonella and 1% of Rickettsia) and was denominated as MEDH. Additionally, a colony of MEAM1 (98% positive for Hamiltonella and 91% positive for Rickettsia) was also tested. The viruses were efficiently transmitted by MED, but transmission efficiency varied among the MED and MEDH, being CpMMV, BGMV and ToCV better transmitted by MEDH. Moreover, transmission efficiency of ToSRV and ToCV by MEDH was even significantly better than MEAM1. We conclude that specimens from B. tabaci MED are good vectors of virus infecting tomato and beans in Brazil and populations with Hamiltonella prevalence increased the virus transmission. UNESP – Faculdade de Ciências Agronômicas Centro de Fitossanidade Instituto Agronômico de Campinas UNESP – Faculdade de Ciências Agronômicas FAPESP: 2014/047289-4 FAPESP: 2017/21588-7 FAPESP: 2017/50222-0 CNPq: 479101/2013-2

Submitted by Eunice Novais (enovais@uepg.br) on 2019-09-25T18:36:33Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) AGHATA CRISTIE REWA CHARNOBAY.pdf: 2307881 bytes, checksum: da0eed1b709018d6c82ee35fc4daf01e (MD5) Made available in DSpace on 2019-09-25T18:36:33Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) AGHATA CRISTIE REWA CHARNOBAY.pdf: 2307881 bytes, checksum: da0eed1b709018d6c82ee35fc4daf01e (MD5) Previous issue date: 2019-02-28 Com menor quantidade de terra e de recursos econômicos os agricultores de base familiar ficaram fora do modelo produtivo baseado nos princípios da revolução verde. Para este grupo, visando desenvolvimento rural sustentável, existe a necessidade de abordagens alternativas. Os sistemas agroflorestais reúnem serviços sociais, econômicos e ambientais e podem ser alternativas para o desenvolvimento e sustentabilidade no meio rural. O objetivo deste trabalho foi avaliar qualidade do solo em sistema agroflorestal familiar utilizando atributos físicos, químicos e biológicos. O estudo foi realizado em propriedade de agricultores familiares localizada no Assentamento Contestado, no município da Lapa – PR. O clima da região é classificado como Cfb e o solo como Cambissolo Háplico Tb Distrófico. Foram selecionadas quatro áreas na propriedade, caracterizadas por diferentes usos e manejo do solo: (a) linha de agrofloresta com tempo de adoção de 4 anos (AL); (b) entrelinha de agrofloresta com tempo de adoção de 4 anos (AE); (c) lavoura de milho com preparo convencional de solo (LA) e (d) área de mata nativa (MT). Foram realizadas amostragens nas profundidades 0-5, 5-10, 10-20 e 20-40 cm para os indicadores físicos densidade do solo, porosidade total, macroporosidade, microporosidade e bioporosidade e os indicadores químicos pH, acidez potencial (H+ + Al3+) e teores de cálcio, magnésio, potássio, fósforo, carbono e alumínio. Na camada 0-10 cm foram mensurados a condutividade hidráulica saturada do solo (Kfs) e os indicadores biológicos carbono da biomassa microbiana, respiração do solo, quociente microbiano e quociente metabólico. Foi realizada também a análise visual da estrutura do solo (AVES). Para as variáveis físicas (com exceção da AVES e da Kfs) e químicas do solo foram empregadas a análise de componentes principais (ACP) e análise hierárquica de agrupamentos (AHA). As demais variáveis foram submetidas às análises de variância e estatística de contrastes ortogonais. A área de mata nativa apresentou características pedogenéticas distintas. A ACP indicou, em todas as camadas, a existência de três grupos com variabilidades diferentes: 1) MT, 2) AL e AE e 3) LA. A AHA confirmou os resultados denotados na ACP. A textura do solo e os atributos químicos de AL e AE foram semelhantes a LA. AE e LA apresentaram valores limitantes de macroporosidade na camada 10-20 cm e 5-10 e 10-20 cm, respectivamente. Nas camadas 0-5 e 5-10 cm, AL e AE apresentaram maior volume de bioporos em relação a LA. Para Kfs, AE foi o manejo que apresentou os menores valores de infiltração, não diferindo de LA. Os atributos microbiológicos não diferiram entre os manejos. A linha e a entrelinha da agrofloresta apresentaram melhor qualidade visual da estrutura do solo comparado à lavoura. With less land area and economic resources, family-based farmers were left out of the productive model based on the green revolution principles. For this group there is a need for alternative approaches aiming at sustainable rural development. Agroforestry systems bring together social, economic and environmental services and can be alternatives for rural development and sustainability. The objective of this study was to evaluate soil quality in a family agroforestry system using physical, chemical and biological indicators. The study was carried out in family farm located in the Contestado Settlement, in Lapa - PR. The climate of the region is classified as Cfb and the soil as a Cambisol. Four areas of the farm were selected, characterized by different soil uses and management: (a) agroforestry row with adoption time of 4 years (AR); (b) agroforestry inter-row with adoption time of 4 years (AI); (c) maize crop with conventional tillage (CT) and (d) native forest (NF). Soil density, total porosity, macroporosity, microporosity and bioporosity, and the chemical indicators pH, potential acidity (H+ + Al3+), calcium, magnesium, potassium, phosphorus, carbon and aluminum contents were measured at the layers 0-5, 5-10, 10-20 and 20-40 cm. In the 0-10 cm layer, saturated soil hydraulic conductivity (Kfs) and the biological indicators microbial biomass carbon, soil respiration, microbial quotient and metabolic quotient were measured. The visual evaluation of soil structure (VESS) was also performed. For the physical variables (with the exception of VESS and Kfs) and chemical variables, the principal component analysis (PCA) and hierarchical group analysis (AHA) were used. The other variables were submitted to analysis of variance and statistics of orthogonal contrasts. The native forest presented distinct pedogenetic characteristics. PCA indicated, in all layers, the existence of three groups with different variability: 1) NT, 2) AR and AI and 3) LA. The AHA confirmed the results reported in the PCA. Soil texture and chemical attributes of AR and AI were similar to CT. AI and CT showed limiting values of macroporosity in the 10-20 cm and 5-10 and 10-20 cm layer, respectively. In the layers 0-5 and 5-10 cm, AR and AI presented higher volume of biopores compared to CT. For Kfs, AI was the management that presented the lowest infiltration values, not differing from CT. Microbiological attributes did not differ between soil managements. The agroforestry row and inter-row presented better visual evaluation of the soil structure compared to the maize crop area.

A demanda por sementes com boa qualidade fisiológica e sanitária, com o propósito de produzir mudas sadias de espécies florestais nativas, cresceu nos últimos anos, em função da restauração de florestas, recuperação de áreas degradadas, recomposição de paisagens e matas ciliares. Poucas pesquisas têm sido desenvolvidas na área de sanidade de sementes, direcionadas à identificação de fungos, à participação destes no processo de deterioração, à condução de testes de patogenicidade e à transmissão de patógenos. Também há poucos estudos sobre tratamento e conservação de sementes das espécies de interesse e, consequentemente, de preservação da diversidade vegetal, especialmente das sementes nativas de matas tropicais. A grande diversidade de espécies dessas formações vegetais demanda esforços ainda maiores para que se possa obter sucesso na identificação e controle dos principais patógenos associados às sementes de espécies florestais. Made available in DSpace on 2019-09-05T00:50:12Z (GMT). No. of bitstreams: 1 2019AlvaroSPPatalogia.pdf: 283344 bytes, checksum: ad35a55e51b30a2133cd6e9f88ae2d10 (MD5) Previous issue date: 2019

To test the hypothesis that the plant stress related elicitor cis-jasmone (cJ) provides protection in soybean pods against the seed-sucking stink bug pest, Euschistus heros, the growth of E. heros on cJ-treated pods was investigated using three soybean cultivars differing in insect susceptibility, i.e. BRS 134 (susceptible), IAC 100 (resistant) and Dowling (resistant). E. heros showed reduced weight gain when fed cJ-treated Dowling, whereas no effect on weight gain was observed when fed other treated cultivars. Using analysis of variance, a three factor (cultivar x treatment x time) interaction was observed with concentrations of the flavonoid glycosides daidzin and genistin, and their corresponding aglycones, daidzein and genistein. There were increases in genistein and genistin concentrations in cJ-treated Dowling at 144 and 120 h post treatment, respectively. Higher concentrations of malonyldaidzin and malonylgenistin in Dowling, compared to BRS 134 and IAC 100, were observed independently of time, the highest concentrations being observed in cJ-treated seeds. Levels of glycitin and malonylglycitin were higher in BRS 134 and IAC 100 compared to Dowling. Canonical variate analysis indicated daidzein (in the first two canonical variates) and genistein (in the first only) as important discriminatory variables. These results suggest that cJ treatment leads to an increase in the levels of potentially defensive isoflavonoids in immature soybean seeds, but the negative effect upon E. heros performance is cultivar-dependent., Graphical abstract cis-Jasmone treatment of soybean pods changes flavonoid levels and negatively affects Euschistus heros growth in a cultivar dependent manner.Image 1, Highlights • cis-Jasmone (cJ) affects levels of defence flavonoids in immature seeds of soybean, Glycine max. • The weight gain of the seed sucking stink bug pest, Euschistus heros, is reduced when fed on cJ-treated cv. Dowling only. • The negative effect of cJ treatment upon E. heros performance is cultivar dependent.

Made available in DSpace on 2018-12-11T16:52:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-08-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The objective of this work was to evaluate the genetic parameters and estimate genetic gains in two populations of Hevea brasiliensis. The first population of the study is originated from the primary forest of Rio Branco – Acre (wild population – PS), and the other population is originated from clonal matrices (improved population – PM). Thirty-seven progenies were evaluated at 23 years for PS. For PM, 31 progenies at eight years of age were evaluated for growth traits and dry rubber yield. Estimates of the genetic parameters were made using the univariate linear mixed-model additive model REML/BLUP and gains in selection by the Multi-Effects Index (MEI) method. The selection strategies of 50%, 40% and 22% of individuals for the character of dry rubber yield (PBS) and stem perimeter (PAP) using the multi-effects index revealed high and low selection gains in both populations. For MP, the strategy of selection between and within progenies was more appropriate and for PS, the individual selection. The gains obtained in the selection were 54% for the PBS character in the improved population of 0.46% for the PAP character for the wild population. Universidade do Estado de Mato Grasso/UNEMAT, CP 08, Rua Prof. Dr. Renato Figueiro Varella Faculdade de Filosofia Ciências e Letras de Ribeirão Preto/USP Faculdade de Engenharia de Ilha Solteira/UNESP Instituto Agronômico de Campinas/IAC Faculdade de Engenharia de Ilha Solteira/UNESP

Made available in DSpace on 2021-07-14T10:51:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-22. Added 1 bitstream(s) on 2021-07-14T11:39:50Z : No. of bitstreams: 1 S1808-16572017000100220.pdf: 253021 bytes, checksum: e4d2891da99db8011f3117d309d60abb (MD5) Bacillus tem sido amplamente estudado e usado para o controle de pragas e doenças. O protocolo adaptado proposto por POLANCZYK (2004) mostrou-se mais eficiente do que o da Organização Mundial de Saúde (WHO, 1985) para isolar cepas edáficas de Bacillus. No entanto, não foi avaliado quanto ao isolamento de estirpes endofíticas, que são muito menos abundantes na natureza e mais difíceis de isolar. Este estudo teve como objetivos comparar dois procedimentos metodológicos para o isolamento de Bacillus, o estabelecido pela OMS (WHO, 1985) e o de POLANCZYK (2004), quanto a sua eficiência para o isolamento de estirpes endofitas e edáficas de Bacillus originárias do interior do tecido radicular de cana-de-açúcar, bem como de amostras de solos associados, coletada de 11 locais; e comparar a densidade de bactérias em ambos os ambientes. As cepas endofíticas e edáficas de Bacillus foram isoladas por ambos os procedimentos. No entanto, o protocolo de isolamento realizado por POLANCZYK (2004) demonstrou-se mais eficiente por gerar maior número de unidades de formação de colônias (CFU) por grama de solo e raiz, indicando que esse procedimento é mais útil, especialmente para isolamento de estirpes endofíticas de Bacillus, que são muito menos abundantes na natureza do que as cepas edáficas, sendo, portanto, mais difíceis de serem isoladas. Usando o protocolo de POLANCZYK (2004), as cepas de Bacillus foram isoladas de todas as amostras de raízes (endofíticas) e de solo (edáficas) de todos os 11 campos, sugerindo que a raiz da planta pode ser outra fonte importante de isolamento de Bacillus além do solo. As densidades mais altas de Bacillus foram isoladas do ambiente edáfico em comparação com o ambiente endofítico, com diferenças significativas quando isoladas pelo método de POLANCZYK (2004). Bacillus has been widely studied and used for the control of pests and diseases. The adapted protocol proposed by POLANCZYK (2004) proved to be more efficient than the one by the World Health Organization (WHO, 1985) to isolate edaphic strains of Bacillus. However, it has not been assessed for isolation of endophytic strains, which are much less abundant in the nature and more difficult to be isolated. This study aimed to compare two methodological procedures for isolation of Bacillus, established by the WHO (1985) and by POLANCZYK (2004), regarding their efficiency for isolation of endophytics and edaphics Bacillus strains from inside the root tissue of sugarcane, as well as from the associated soil sample, collected from 11 locations; and to compare the density of bacteria in both environments. Endophytic and edaphic strains of Bacillus were isolated by both procedures. However, the isolation protocol performed by POLANCZYK (2004) made more efficient by having a greater number of colony forming units (CFU) per gram of soil and root indicating that this procedure is more useful, especially for isolation of endophytic strains of Bacillus, which are much less abundant in the nature than edaphic strains, being therefore more difficult to be isolated. Using the Polanczyk protocol (2004), Bacillus strains were recovered from all roots (endophytic) and soil (edaphic) samples of all the 11 fields, suggesting that the plant root may be another important source for isolation of Bacillus besides the soil. Higher densities of Bacillus were isolated from the edaphic environment compared with the endophytic environment, with significant differences when isolated by Polanczyk method (2004). Instituto Biológico, Centro Experimental Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrarias e Veterinárias Centro Universitario de Gurupi Instituto Agronômico de Campinas, Centro de Solos e Recursos Agroambientais Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrarias e Veterinárias

Made available in DSpace on 2018-12-11T17:17:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-12-16 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The average yield of upland rice under no-tillage system (NTS), a sustainable soil management, is lower than in conventional tillage (one plowing and two disking). One of the reasons given for this drop in crop grain yield would be the low-nitrate assimilation capacity of rice seedlings, due to the low activity of the nitrate reductase (NR) enzyme in the early development phase. A greenhouse experiment was conducted to evaluate the effects of the soil acidic and nitrogen source in the micronutrient concentrations, NR activity and grain yield of upland rice growing under NTS. The soil used in the experiment was an Oxisol. The experimental design was completely randomized in a factorial 3 × 4. Treatments consisted of three levels of soil acidity (high, medium, and low) combined with four nitrogen sources (nitrate, ammonium, ammonium + nitrification inhibitor, and control–without N fertilization). The reduction of soil acidity reduced the concentration of zinc and manganese in rice plants. Generally, the activity of the NR enzyme was higher in plants grown in soils with low acidity and fertilized with calcium nitrate. There was a greater response in growth and yield in rice plants grown in soils with high acidity. Under medium acidity, rice plants grown with ammonium sulfate were more productive (no differences were detected with the addition of the nitrification inhibitor). Departamento de Agronomia Universidade do Oeste Paulista–UNOESTE Campus II Faculdade de Ciências Agronômicas Departamento de Produção Vegetal (Agricultura) Universidade Estadual Paulista (UNESP) Empresa Brasileira de Pesquisa Agropecuária (Embrapa Arroz e Feijão) Centro de Solos e Recursos Ambientais Instituto Agronômico de Campinas Departamento de Química e Bioquímica Universidade Estadual Paulista (UNESP) Instituto de Biociências Faculdade de Ciências Agronômicas Departamento de Produção Vegetal (Agricultura) Universidade Estadual Paulista (UNESP) Departamento de Química e Bioquímica Universidade Estadual Paulista (UNESP) Instituto de Biociências FAPESP: 2008/05573-0 FAPESP: 2008/05641-6

Made available in DSpace on 2015-08-26T19:19:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-06-01. Added 1 bitstream(s) on 2015-08-31T13:04:47Z : No. of bitstreams: 1 S0001-37652015000200943.pdf: 701047 bytes, checksum: 5acdbf08edb890484f711fe744db05b1 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação para o Desenvolvimento da UNESP (FUNDUNESP) Os óleos essenciais de folhas e inflorescências de Lippia origanoides Kunth apresentam potencial aromático e medicinal e têm sido utilizados no tratamento de diversas doenças, incluindo melanoma. No Brasil, L. origanoides é comumente encontrada no campo cerrado e no cerrado stricto sensu, fisionomias caracterizadas principalmente pelas diferentes condições de luminosidade às quais as plantas de médio e pequeno porte estão sujeitas. Nosso objetivo foi investigar a densidade de tricomas glandulares e o rendimento e a composição química do óleo essencial em folhas e inflorescências de L. origanoides do campo cerrado e do cerrado stricto sensu. Para análise de densidade glandular, folhas e inflorescências foram processadas segundo técnicas convencionais em microscopia eletrônica de varredura. Os óleos essenciais de folhas e inflorescências foram obtidos por hidrodestilação e identificados com cromatografia gasosa. Brácteas e sépalas mostraram a maior densidade glandular, seguidas por pétalas e folhas. A densidade glandular na face abaxial do limbo foliar foi maior em indivíduos do campo cerrado. Em ambas as fisionomias, o rendimento de óleos essenciais foi maior nas inflorescências em comparação com folhas. A composição química dos óleos essenciais variou entre os indivíduos de diferentes áreas e entre indivíduos da mesma população. Nossos resultados demonstraram a plasticidade química de L. origanoides sugerindo a importância do monitoramento de seu uso popular. The essential oils from leaves and inflorescences of Lippia origanoides Kunth present aromatic and medicinal potential and have been used to treat several diseases, including melanoma. In Brazil, L. origanoides is commonly found in campo cerrado and cerrado stricto sensu, physiognomies featured mainly by the differential light conditions to which short and medium-sized plants are subjected. Our aim was to investigate the glandular trichome density and the yield and chemical composition of the essential oils in leaves and inflorescences of L. origanoides from campo cerrado and cerrado stricto sensu. For glandular density analysis, leaves and inflorescences were processed according to conventional techniques for scanning electron microscopy. The essential oils of leaves and inflorescences were obtained by hydrodistillation and identified with gas chromatography. Bracts and sepals showed the highest glandular density, followed by petals and leaves. The glandular density in the abaxial leaf surface was higher in individuals from the campo cerrado. In both populations the essential oil yield was higher in inflorescences than in leaves. The chemical composition of the essential oils varied among individuals from different areas and inside a same population. Our results demonstrated the chemical plasticity of L. origanoides suggesting the importance of monitoring its popular use. Universidade Estadual Paulista Instituto de Biociências de Botucatu Departamento de Botânica Instituto Agronômico de Campinas Laboratório de Produtos Naturais Universidade Estadual Paulista Instituto de Biociências de Botucatu Departamento de Botânica FAPESP: 2008/55434-7 FUNDUNESP: 0504/011/14

Made available in DSpace on 2016-07-07T12:36:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2015. Added 1 bitstream(s) on 2016-07-07T12:45:07Z : No. of bitstreams: 1 S1516-05722015000100150.pdf: 427286 bytes, checksum: 20ed0b4ec077d6f077f1197ae8e435bc (MD5) O presente trabalho visou avaliar a influência de diferentes tempos de extração no teor e composição química do óleo essencial da espécie Pothomorphe umbellata. Folhas de pariparoba foram coletadas em Cajamar – SP, no mês de junho de 2006. O delineamento experimental adotado foi inteiramente casualizado, com seis tempos de extração (90, 120, 150, 180, 210 e 240 minutos) e 4 repetições. A extração do óleo essencial foi realizada por hidrodestilação e a análise química em CG-EM. O tempo de 180 minutos proporcionou maior rendimento do óleo essencial (0,42%), enquanto a composição química não foi influenciada pelos diferentes tempos de destilação. As principais substâncias identificadas foram: D-germacreno (65,5%), alfa-selineno (9,7%), trans-cariofileno (7,5%), e espatulenol (6,58%). Conclui-se que o tempo de extração afeta diretamente o rendimento e a proporção das substâncias químicas, recomendando-se a extração em até 180 minutos. The present work aimed to evaluate the influence of different times of extraction in the yield and chemical composition of the essential oil of “pariparoba” (Pothomorphe umbellate). Leaves of “pariparoba” were collected in the municipal district of Cajamar – state of São Paulo, Brazil, in June 2006. The adopted experimental design was completely randomized, with six times of extraction (90, 120, 150, 180, 210, and 240 minutes) and 4 repetitions. The extraction of the essential oil was accomplished by hydrodistillation, and the chemical analysis was performed in GC-MS. The time of extraction of 180 minutes provided greater yield of the essential oil (0.42%), and its chemical composition was not influenced by the different times of hydrodistillation. The main substances showed are D-germacrene (65.5%), alpha-selinene (9.7%), trans-caryophyllene (7.5%) and spathulenol (6.58%). The results of this work showed that the time of extraction affects the yield and the proportion of the chemical substances and it is recommended the extraction in up to 180 minutes. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agronômicas (FCA), Departamento de Horticultura, Botucatu, SP, Brasil Instituto Agronômico de Campinas (IAC), Laboratório de Produtos Naturais, Campinas, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agronômicas (FCA), Departamento de Horticultura, Botucatu, SP, Brasil

The primary focus of tropical forest restoration has been the recovery of forest structure and tree taxonomic diversity, with limited attention given to genetic conservation. Populations reintroduced through restoration plantings may have low genetic diversity and be genetically structured due to founder effects and genetic drift, which limit the potential of restoration to recover ecologically resilient plant communities. Here, we studied the genetic diversity, genetic structure and differentiation using single nucleotide polymorphisms (SNP) markers between restored and natural populations of the native tree Casearia sylvestris in the Atlantic Forest of Brazil. We sampled leaves from approximately 24 adult individuals in each of the study sites: two restoration plantations (27 and 62 years old) and two forest remnants. We prepared and sequenced a genotyping-by-sequencing library, SNP markers were identified de novo using Stacks pipeline, and genetic parameters and structure analyses were then estimated for populations. The sequencing step was successful for 80 sampled individuals. Neutral genetic diversity was similar among restored and natural populations (AR = 1.72 ± 0.005; HO = 0.135 ± 0.005; HE = 0.167 ± 0.005; FIS = 0.16 ± 0.022), which were not genetically structured by population subdivision. In spite of this absence of genetic structure by population we found genetic structure within populations but even so there is not spatial genetic structure in any population studied. Less than 1% of the neutral alleles were exclusive to a population. In general, contrary to our expectations, restoration plantations were then effective for conserving tree genetic diversity in human-modified tropical landscapes. Furthermore, we demonstrate that genotyping-by-sequencing can be a useful tool in restoration genetics. Made available in DSpace on 2018-12-12T00:03:58Z (GMT). No. of bitstreams: 1 GenomicDiversity.pdf: 1696141 bytes, checksum: d76d48e836129aae93b94e28ce94de2d (MD5) Previous issue date: 2018-12-11

Made available in DSpace on 2018-12-11T16:47:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-04-24 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Citrus sudden death (CSD) has caused the death of approximately four million orange trees in a very important citrus region in Brazil. Although its etiology is still not completely clear, symptoms and distribution of affected plants indicate a viral disease. In a search for viruses associated with CSD, we have performed a comparative high-throughput sequencing analysis of the transcriptome and small RNAs from CSD-symptomatic and -asymptomatic plants using the Illumina platform. The data revealed mixed infections that included Citrus tristeza virus (CTV) as the most predominant virus, followed by the Citrus sudden death-associated virus (CSDaV), Citrus endogenous pararetrovirus (CitPRV) and two putative novel viruses tentatively named Citrus jingmen-like virus (CJLV), and Citrus virga-like virus (CVLV). The deep sequencing analyses were sensitive enough to differentiate two genotypes of both viruses previously associated with CSD-affected plants: CTV and CSDaV. Our data also showed a putative association of the CSD-symptomatic plants with a specific CSDaV genotype and a likely association with CitPRV as well, whereas the two putative novel viruses showed to be more associated with CSD-asymptomatic plants. This is the first high-throughput sequencing-based study of the viral sequences present in CSD-affected citrus plants, and generated valuable information for further CSD studies. Instituto de Biociências da Universidade Estadual Paulista (Unesp) Centro de Citricultura Sylvio Moreira Instituto Agronômico de Campinas Department of Plant Pathology University of California Institute for Sustainable Plant Protection (IPSP) CNR Instituto de Biociências da Universidade Estadual Paulista (Unesp)

Peanut is an oilseed crop of great importance for Brazilian agribusiness. A major factor affecting its production is pest incidence, mainly thrips. This study aimed to evaluate the potential for resistance to Enneothrips flavens in genotypes derived from the cross between IAC 503 and the amphidiploid (A. magna x A. cardenasii)4x and to estimate the genetic and phenotype parameters in these genotypes, allowing for better targeting in the selection. The experiments were conducted in a Federer augmented block design with additional checks in two generations (F3 and F4). Resistance to thrips was evaluated by its natural infestation and the symptoms of attacks by the insect. They were also evaluated using agronomic trait indicators of interspecific segregating with cultivated species. The results indicated that the selected progeny exhibited high resistance to thrips compared to commercial genotypes, and they had the amphidiploid as the insect resistance source. Some progenies selected as resistant also had good production traits, but with the degree of suitability to the A. hypogaea L. genotypes still low, the use of a backcross as an alternative for the introgression of resistance genes and the consequent recovery of adapted genotypes of superior recurring parents is suggested. Made available in DSpace on 2019-05-22T01:08:35Z (GMT). No. of bitstreams: 1 18078621asagr390300339.pdf: 656992 bytes, checksum: 4a305575133c1794064a55536b614f10 (MD5) Previous issue date: 2017

Although there are evidences that the proper supply of mineral nutrients to plants relieves water stress, little is known on the approach of how the drought affects the absorption and accumulation of nutrients by distinct sugar cane genotypes, or in different parts of a same plant. Thus, the objective of this study was to determine the content and accumulation of N, P and K in the aerial part of plant from three genotypes of sugar cane, submitted to three water potentials in the soil, and check the relationship of these variables with the tolerance of plants to prolonged drought. In order to access this objective, an experiment under greenhouse conditions, comprised by a fatorial 3 × 3, in a randomized block design, and four replicates was carried out. After 90 days from treatment imposition, the plant transpiration rate, plant dry mass, concentration of N, P and K were determined in leaves and culms, as well as in total plant shoot were measured. It was found that tolerance to drought in sugar cane is related to higher levels of N and K in the leaves and stems, and larger accumulations of K and P in the plant shoot. There is high positive correlations among accumulation of N, P and K in the plant shoot and dry matter production by plants submitted to drought. There are intermediate correlations among plant transpiration and nutrient uptake by plants under drought. Plants of the genotype SP81-3250 are more tolerante to prolonged drought, than the RB855453 and IACSP95-5000 plants. RESUMO Embora existam evidências de que o adequado fornecimento de nutrientes minerais para plantas alivia o estresse hídrico, pouco se sabe sobre a abordagem de como a seca afeta a absorção e acumulação de nutrientes por distintos genótipos de cana-de-açúcar, ou por diferentes partes de uma mesma planta. Assim, o objetivo deste estudo foi determinar o teor e acúmulo de N, P e K na parte aérea da planta de três genótipos de cana-de-açúcar, submetidos a três potenciais hídricos do solo e verificar se existe relação dessas variáveis com a tolerância das plantas ao déficit hídrico prolongado. Para isso, foi realizado um experimento em casa de vegetação, em um esquema fatorial 3×3, em delineamento de blocos casualizados, com quatro repetições. Após 90 dias da imposição dos tratamentos, foi determinada a massa seca e a taxa de transpiração das plantas, a concentração de N, P e K nas folhas e colmos, além do acúmulo desses nutrientes na parte aérea das plantas. Verificou-se que a tolerância à seca em cana está relacionada com níveis mais altos de N e K nas folhas e caules e maiores acumulações de K e P na parte aérea das plantas. Existem fortes correlações positivas entre acúmulo de N, P e K na parte aérea das plantas e produção de matéria seca. Existem correlações intermediárias entre a transpiração da planta e absorção de nutrientes pelas plantas sob seca. Plantas do genótipo SP81-3250 são mais tolerantes à seca prolongada, que as plantas RB855453 e IACSP95-5000.

A fração da área coberta pelo dossel (fc) tem sido empregada, em diferentes regiões, para a estimativa do coeficiente de cultivo (Kc) de videiras. O presente trabalho teve por objetivo estimar os valores de Kc de videiras destinadas à produção de uvas para elaboração de suco com base nos valores de fc, nas condições do noroeste paulista. As avaliações foram realizadas de agosto a novembro de 2016 no município de Votuporanga (SP), com as cultivares Isabel Precoce e BRS Carmem enxertadas sobre os porta-enxertos ?IAC 572? e ?IAC 766?. As videiras foram conduzidas no sistema espaldeira e irrigadas por microaspersores. Os valores de fc foram obtidos ao longo do ciclo da cultura e correlacionados à soma térmica diária (ST) por meio de modelos de regressão. Os coeficientes de cultivo (Kc), foram obtidos em função da fc com base no modelo de Allen e Pereira (2009). Para a cultivar BRS Carmem, os valores de fc em função de ST cresceram de forma contínua, segundo um modelo quadrático, enquanto que, para a cultivar Isabel Precoce, os dados se ajustaram melhor ao modelo de Mitscherlich, apresentando um rápido crescimento inicial e se estabilizando após 500 °C dia, aproximadamente. Os valores médios estimados de Kc para as cultivares Isabel Precoce e BRS Carmem foram iguais a 0,81 e 0,74, respectivamente. O modelo empregado nas avaliações permite a realização de estimativas dos valores de Kc para diferentes condições de cultivo. Made available in DSpace on 2017-09-21T00:12:11Z (GMT). No. of bitstreams: 1 Conceicaoetal2017.pdf: 813721 bytes, checksum: 539a07ef3b5eba01d2e8ceb987610260 (MD5) Previous issue date: 2017-09-19

Made available in DSpace on 2015-11-03T15:27:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-07-01. Added 1 bitstream(s) on 2015-11-04T10:15:35Z : No. of bitstreams: 1 S0006-87052014000300006.pdf: 729110 bytes, checksum: 82f4275f27772c81e39a75c3a22a592c (MD5) O objetivo deste trabalho foi avaliar as famílias e destacar as progênies superiores de cana-de-açúcar oriundas de 38 cruzamentos biparentais para os seguintes atributos: tonelada de colmos por hectare (TCH), toneladas de biomassa por hectare (TBIOH), brix (% caldo da cana), teor de fibra, pureza, pol e açúcar total recuperável (ATR). Os dados foram analisados via modelos mistos REML/BLUP, em que o REML (Máxima Verossimilhança Restrita) permitiu estimar parâmetros genéticos e BLUP (Melhor Predição Linear Não Viciada) permitiu predizer os valores aditivos e genotípicos. A melhor família para os atributos TCH e TBIOH foi a 41, cujos parentais são as cultivares IACSP022019 x CTC9. Na seleção individual para TCH, a planta de n. 3 do bloco 2, do cruzamento 78 foi a que apresentou o melhor resultado. Para TBIOH, a planta n. 33, do bloco 1, da família 41 foi superior. As famílias 40, 41, 43, 68, 69, 79, 91, 92 e 147 foram superiores para as variáveis brix, pol, pureza e ATR, enquanto que as famílias 85, 147, 148, 149, 161, 163, 177, 178, 179 e 183 foram superiores para fibra. A família 147, cujos parentais são IACSP042286 x IACSP963055, mostrou três progênies entre as dez melhores tanto para brix quanto para fibra, o que identifica essa combinação como potencial fonte de progênies para produção de bioenergia. The objective of this study was to assess families and highlight the superior progenies of sugarcane originating from 38 biparental crosses for the following attributes: tons of cane per hectare (TCH), tons of biomass per hectare (TBIOH), brix (% cane juice), fiber content, purity, pol and total recoverable sugar (TRS). The data were analyzed by mixed model REML / BLUP in the REML (Restricted Maximum Likelihood) allowed us to estimate genetic parameters and BLUP (best linear unbiased prediction) to predict the additive and genotypic values. The best family for the attributes TCH and TBIOH was 41, whose parents are cultivars IACSP022019 x CTC9. In individual selection for TCH, the plant number 3 of Block 2, the crossing 78, showed the best results. To TBIOH the plant number 33, Block 1, family 41, showed the best results. Families 40, 41, 43, 68, 69, 79, 91, 92 and 147, were higher for the variables brix, pol, purity, and ATR, where as 85 families, 147, 148, 149, 161, 163, 177, 178, 179, and 183 were higher for fiber. The family 147 whose parents are IACSP042286 x IACSP963055, showed three progenies ranked among the top ten for both brix, and for fiber, which identifies the combination as a potential source of progenies for bioenergy production. Instituto Agronômico de Campinas, Centro de Cana Universidade Estadual Paulista, Departamento de Ciências Exatas, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal

Somatic embryogenesis is an advantageous tool in the commercial production of micropropagated anthurium plantlets. As such, the aim of this study was to establish a protocol for the induction of somatic embryogenesis in Jureia and Luau cultivars. Defoliated nodal segments, 1.0 cm in length and containing one bud, were used as explants. The experimental design was completely randomised, in a 2 x 3 x 5 factorial scheme (cultivar: Jureia and Luau x auxin: 2,4-D, NAA and Picloram x concentration: 0, 2.5, 5.0, 7.5, and 10.0 μM), with 30 treatments in a scheme of plots split over time (15, 30, 45, 60, 75 and 90 days). The anatomy and percentage of embryogenic callus formation were analysed. The structures formed, analysed by scanning electron microscopy, corresponded to embryogenic calli. The Luau cultivar was superior in forming embryogenic calli. For the two cultivars, among the auxins under study, NAA demonstrated a greater induction potential for somatic embryogenesis, with the concentration of 7.5 μM giving the highest mean values. The 90-day evaluation period showed the maximum formation of embryogenic calli; however, mean values were fairly similar to the 75-day evaluation period. To induce embryogenic calli, therefore, it is suggested that the nodal segments be inoculated into a culture medium with added NAA growth regulator at a concentration of 7.5 μM, and that the explants remain in this medium for 75 days after inoculation.

Made available in DSpace on 2019-10-04T11:57:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-09-01 National Science Foundation (NSF) U.S. Department of Agriculture (USDA) National Science Foundation Graduate Research Fellowship Program A novel flavi-like virus tentatively named Diaphorina citri flavi-like virus (DcFLV) was identified in field populations of Diaphorina citri through small RNA and transcriptome sequencing followed by reverse transcription (RT)-PCR. We report here the complete nucleotide sequence and genome organization of DcFLV, the largest flavi-like virus identified to date. Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA Univ Estadual Paulista, Inst Biociencias Botucatu, Botucatu, SP, Brazil Ctr APTA Citros Sylvio Moreira, Inst Agron Campinas, Lab Biotecnol, Cordeiropolis, SP, Brazil CNR, Inst Sustainable Plant Protect IPSP, Turin, Italy Univ Estadual Paulista, Inst Biociencias Botucatu, Botucatu, SP, Brazil National Science Foundation (NSF): 1148897 U.S. Department of Agriculture (USDA): 13-002NU-781 U.S. Department of Agriculture (USDA): 2015-70016-23011 National Science Foundation Graduate Research Fellowship Program: 1148897

Made available in DSpace on 2018-12-11T17:01:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-05-01. Added 1 bitstream(s) on 2021-07-15T15:02:50Z : No. of bitstreams: 1 S0103-84782016000500825.pdf: 293319 bytes, checksum: c123bc544475e757671cefe23edff3fc (MD5) Citrus black spot (CBS) caused by Guignardia citricarpa is one of the most serious Brazilian citrus diseases. This study aims to assess the interference of three application volumes in spray deposition citrus fruit, as well as fruit growth and rainfall effects on spray deposit reduction during the CBS control period. The experiment was carried out in a commercial citrus orchard, with sixteen-year-old trees of the Valencia variety, in Mogi Guaçu, São Paulo State, Brazil. The spray volumes were: 3.5 (1333L ha-1), 4.5 (1714L ha-1) and 8.5 (3238L ha-1) litres per tree, sprayed by an airblast sprayer using fungicides at recommended periods for disease control. The spray deposition quantification and residue was done by spectrophotometry using a copper oxychloride tracer. Samples were collected in three height zones of the tree (top, middle and bottom) and placed between trees on line plantation. Spray depositions were significantly smaller in the first application as a consequence of reduced fruit size. The spray losses on average for each day of rainfall ranged from 4.0 to 5.7%. There was no significant difference between application volumes regarding spray deposition on citrus fruit,which makes possible the reduction of application volumes, however, it is necessary to improve spraying techniques for the top zone of the citrus tree. Departamento de Proteção Vegetal Defesa Fitossanitária Universidade Estadual Paulista (UNESP) Centro de Engenharia e Automação (CEA) Instituto Agronômico de Campinas (IAC) Departamento de Engenharia Agronômica Universidade Estadual Paulista (UNESP), Campus de Dracena Departamento de Proteção Vegetal Defesa Fitossanitária Universidade Estadual Paulista (UNESP) Departamento de Engenharia Agronômica Universidade Estadual Paulista (UNESP), Campus de Dracena

Made available in DSpace on 2021-07-14T10:51:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-12-15. Added 1 bitstream(s) on 2021-07-14T11:39:48Z : No. of bitstreams: 1 S1808-16572016000100233.pdf: 529930 bytes, checksum: 5d0865df9570dada2fa6d85e771f3748 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) O diagnóstico específico e a avaliação da intensidade da infecção helmíntica em aves são fundamentais em estudos de eficácia e determinação de doses de medicamentos direcionados ao seu controle. O presente trabalho avaliou a aplicação e adaptação da metodologia de Mello e Campos, descrita originalmente para diagnóstico parasitológico em cães, na recuperação de escólices de cestódeos parasitos de aves domésticas (Gallus domesticus ). Foram empregadas 52 aves naturalmente infectadas e oriundas de produções rurais, as quais foram submetidas à necropsia parasitológica, adaptando-se a metodologia Mello e Campos. O método consistiu na realização de quatro etapas: conteúdo, imersão, raspado e avaliação. O número de escólices recuperadas por ave variou de 1 a 4.345, e o maior número de escólices foi recuperado do material oriundo da etapa de imersão. As espécies de cestódeos identificadas foram Amoebotaenia cuneata , Choanotaenia infundibulum , Hymenolepis sp., Raillietina tetragona , Raillietina echinobothrida e Raillietina cesticillus . Os resultados foram avaliados estatisticamente, concluindo-se que a metodologia adotada é eficaz para a recuperação de cestódeos de aves, uma vez que possui a etapa de imersão, que permite a recuperação mais eficiente de escólices. The specific diagnosis and evaluation of the intensity of avian helminth infections are essential for efficacy studies and the determination of drug doses targeted to their control. This study evaluated the Mello and Campos method, originally described for parasitological diagnosis in dogs, in the recovery of scolices from cestode parasites of poultry (Gallus domesticus ). A total of 52 naturally infected birds obtained from farms underwent parasitological necropsy using the Mello and Campos method. The method consisted of four steps: content, soaking, scraping and evaluation. The number of scolices recovered per bird ranged from 1 to 4,345, and the highest number of scolices was recovered from material derived from the soaking step. The cestodes species diagnosed were Amoebotaenia cuneata , Choanotaenia infundibulum , Hymenolepis sp., Raillietina tetragona , Raillietina echinobothrida and Raillietina cesticillus . The Mello and Campos method, originally used to test for helminths in dogs, was effective in avian cestode testing because it includes a soaking step, which enables a more efficient recovery of scolices. Instituto Biológico, Laboratório de Parasitologia Animal Instituto Agronômico de Campinas Universidade Camilo Castelo Branco Universidade Estadual Paulista Júlio de Mesquita Filho, School of Veterinary Medicine Universidade Estadual Paulista Júlio de Mesquita Filho, School of Veterinary Medicine FAPESP: 2012/18929-3; 2013/03599-0; 2014/04097-1

Made available in DSpace on 2018-11-12T17:27:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2016. Added 1 bitstream(s) on 2021-07-14T17:51:34Z : No. of bitstreams: 1 S1808-16572016000100205.pdf: 633613 bytes, checksum: 485805fbee4fc3b2be4aa0d1b0242f38 (MD5) RESUMO: Avaliou-se a resistência de quatro cultivares de amendoim de hábito de crescimento rasteiro e cinco cultivares de hábito de crescimento ereto ao dano de Stegasta bosquella (Chambers, 1875) (Lepidoptera: Gelechiidae) em condições de campo na safra das águas, nos anos 2009/2010 e 2010/2011. Os cultivares utilizados nos experimentos foram: IAC 147, IAC 213, IAC Caiapó e IAC Runner 886 (porte rasteiro) e IAC Tatu ST, IAC 5, IAC 8112, IAC 22 e IAC 88-1 (porte ereto). O delineamento adotado foi em blocos ao acaso, com parcelas subdivididas ao longo do tempo, e quatro repetições. Para a infestação de S. bosquella , foram realizadas avaliações semanais dos 18 aos 67 dias após a emergência (DAE), com contagem de lagartas e avaliações de danos. Para a contagem do número de brotos por planta, coletaram-se 5 plantas por parcela a partir dos 30, 60 e 90 DAE. Independentemente do cultivar, as maiores infestações de S. bosquella ocorrem dos 53 aos 67 DAE. O cultivar IAC Runner 886 apresenta a maior emissão de brotos apicais, uma característica desejável na defesa da planta em relação à S. bosquella . ABSTRACT: We evaluated the strength of four growth habit of peanut cultivars creeping and five cultivars of erect growth habit to damage Stegasta bosquella (Chambers, 1875) (Lepidoptera: Gelechiidae) under field conditions during the rainy season, in the years 2009/2010 and 2010/2011. The cultivars used in the experiments were: IAC 147, IAC 213, IAC Caiapó and IAC Runner 886 (runner) and IAC Tatu ST, IAC 5, IAC 8112, IAC 22 e IAC 88-1 (erect). The design adopted was randomized blocks with split plot over time, and four replications. For infestation of S. bosquella , weekly evaluations were done 18 to 67 days after emergence (DAE), with caterpillars count and damage assessments. For counting the number of shoots per plant, they collected up five plants per plot from the 30, 60 and 90 DAE. Regardless of cultivating major infestations of S. bosquella occur from 53 to 67 DAE. The IAC Runner 886 cultivar has the highest emission apical shoots a desirable feature in plant defense in relation to S. bosquella . Universidade Estadual Paulista Instituto Federal Goiano Instituto Agronômico de Campinas Universidade Estadual Paulista

Made available in DSpace on 2018-12-11T16:42:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01. Added 1 bitstream(s) on 2019-10-09T18:28:38Z : No. of bitstreams: 1 S1516-635X2016000100163.pdf: 490518 bytes, checksum: 50e341f79823a8bbf1079fb58b4c8309 (MD5) The Brazilian poultry industry is an outstanding national and international agribusiness sector. Among the Brazilian states, São Paulo is the largest producer of commercial eggs and the fourth largest producer and exporter of chicken meat. Alternatively, semiintensive and/or organic poultry production have also obtained a significant share of the domestic market as a result of consumer demand. Helminths affect the performance of the birds, causing significant direct or indirect losses. The objective of the present study was to identify the main helminth species present in chickens reared in 17 municipalities of the state of São Paulo. In total, 359 adult birds were investigated. The birds were reared in different housing systems and were obtained from 69 farms in the selected regions. The birds were submitted to procedures for the detection and identification of helminth parasites, following international standards. The evaluation of the small intestine employed the Mello-Campos method (Mello & Campos, 1974), which allows better recovery of cestode scolices attached to the intestinal mucosa. Stereomicroscopy was used to evaluate the collected materials, and light microscopy was used to identify the species based on their morphological characteristics. The following helminth species were diagnosed in chickens reared in 17 municipalities of the state of São Paulo: nematodes (Ascaridia galli, Capillaria sp., Cheilospirura hamulosa, Heterakis gallinarum, Oxyspirura mansoni, and Strongyloides sp.), cestodes (Amoebotaenia cuneata, Choanotaenia infundibulum, Hymenolepis sp., Raillietina cesticillus, Raillietina echinobothrida, and Raillietina tetragona), and trematodes (Zygocotyle lunata and Postharmostomum commutatum). Laboratório de Parasitologia Animal Instituto Biológico-IB/APTA/SAA Instituto Agronômico de Campinas-IAC/ APTA/SAA Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária UNESP Departamento de Clínica Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária UNESP Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária UNESP Departamento de Clínica Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária UNESP

The complex of leaf diseases that affect the passion fruit culture, especially the passion fruit (Passiflora allata) compromise the productivity and quality of fruits and their photosynthetic surfaces. However, P. allata is also used as a medicinal product, the leaves being the part used for this purpose. Thus, the focus of studies on the agronomic management of P. allata for the production of pharmaceutical raw materials differs, at least in part, from the focus on fruit production. Therefore, we evaluated the effect of broth and sulfoccal lime in different concentrations and ages of plants on diseases causing leaf blight of P. allata. The experiment was carried out under randomized block field conditions with five treatments and five replications of 10 plants each. The treatments were: a) Bordeaux syrup at 0.5 and 1.0%; b) 0.5 and 1.0% sulfocalcium syrup from a 27 ° Baumé matrix solution and c) control (without spraying). Treatment with 05% sulfocalcium spray promoted the lowest disease index and the highest relative control efficiency associated with the lower rate of disease development with the age of the crop., O complexo de doenças foliares que acometem a cultura do maracujá, em especial, a do maracujá-doce (Passiflora allata) comprometem a produtividade e qualidade de frutos e de suas superfícies fotossintética. Contudo, o P. allata, é utilizado também como medicinal, sendo as folhas, a parte utilizada para este fim. Desta forma, o enfoque de estudos voltados para o manejo agronômico do P. allata para produção de matéria prima para fim farmacêutico, diverge, pelo menos em parte, do enfoque da produção de frutos. Portanto, avaliou-se o efeito das caldas bordaleza e sulfocálcica em diferentes concentrações e idades de plantas sobre doenças causadoras de manchas foliares do P. allata. O experimento foi instalado em condições de campo em blocos ao acaso com cinco tratamentos e cinco repetições de 10 plantas cada. Os tratamentos foram: a) calda bordalesa a 0,5 e 1,0%; b) calda sulfocálcica a 0,5 e 1,0% a partir de solução matriz a 27º Baumé e c) testemunha (sem pulverização). O tratamento com calda sulfocalcica a 05 % promoveu o menor índice de doença e a maior eficiência relativa de controle associada à menor taxa de desenvolvimento da doença com a idade do cultivo. &nbsp

International audience; Biochar has been proposed as an effective soil amendment for the management of metal(loid)s [1] via a number of mechanisms: directly sorbed on its surface, by the occlusion of soil particles and, in particular, by modifying soil chemistry such as pH or dissolved organic carbon (DOC). An increase of soil pH induced by biochar has been shown to reduce metal solubility, while a release of labile compounds from biochar has resulted in soil metal leaching [2-4]. Different results may therefore be expected depending on the use of "fresh" or "aged" biochar, subject to weathering which can modify the surface and chemical properties of the biochar. Column leaching experiments were conducted in August 2014 by Frédéric Rees and Dr. Luke Beesley with the help of Aline Peregrina Puga at the James Hutton Institute of Aberdeen (UK), with the support of COST Action TD1107. One agricultural soil contaminated by repeated sewage sludge amendments was tested together with one biochar, used as purchased or aged by exposure to natural rainfall. Instead of amending soils with biochar in a single column, a new protocol was tested (Fig. 1), using one column for each material, linked in a loop system so that the retention of metals from soils by the biochar and the modifications of the biochar on the eluent fed back directly to the contaminated soils could be elucidated. Samples of the leaching solution were regularly collected after the soil column and the biochar column, which enabled the chemical evolution of the soil-biochar systems towards equilibrium to be monitored. Chemical equilibration between soils and biochar was fast, as seen by the identical final pH at the different sampling points of the system. Fresh and aged biochar retained metals released from the agricultural soil and decreased further metal release by increasing soil pH. Fresh biochar became however saturated with Zn at the end of the experiment, while aged biochar did not, indicating that aged biochar had a greater sorption capacity for metals. In conclusion, this work indicated that the simple ageing of biochar before its use as a sorbent amendment can increase its metal retention capabilities.

Made available in DSpace on 2018-12-11T17:25:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-08-22 Brazil is the largest producer of oranges (Citrus sinensis) in the world. The nutrient management of tree orchards is designed from experiments with a limited number of varieties. This knowledge is transferred to other varieties by diagnosing tissue nutrient composition and tree demand. Compositional data analysis has been first applied to tissue analysis of agricultural crops using centered log ratios with compositional nutrient diagnosis (CND-clr). The isometric log ratio (ilr) transformation is a new approach based on binary nutrient ratios and the principle of orthogonality (CND-ilr). We analyzed eleven nutrients: nitrogen (N), sulfur (S), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), boron (B), copper (Cu), zinc (Zn), manganese (Mn), and iron (Fe) in leaf tissue samples across 108 commercial plots (thirty-one grow Valencia, twenty-two Hamlim, twenty Pera, and thirty-five Natal). Nutrients were partitioned between macro- and micronutrients as well as anionic and cationic species. The effect size of varieties over Valencia was quantified by the mean and standard deviation of ilr values across ilr coordinates. Specific varietal nutrient profiles and ilr norms were defined. The nutrient profile of orange varieties could be classified into homogeneous groups to take advantage of fertilizer trials conducted on varieties of the same group. The Aitchison distance and a perturbation vector could be instrumental for diagnostic purposes and nutrient management. Universidade Estadual Paulista “Júlio de Mesquita Filho,” Instituto Agronômico de Campinas IAC / Centro de Citricultura Sylvio Moreira Université Laval / ERSAM Departamento des Sols et de Génie Agroalimentaire UNESP Departamento de Solos e Adubos Universidade Estadual Paulista “Júlio de Mesquita Filho,” UNESP Departamento de Solos e Adubos

Made available in DSpace on 2015-08-26T19:21:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-06-01. Added 1 bitstream(s) on 2015-08-31T13:04:11Z : No. of bitstreams: 1 S1984-70332015000200087.pdf: 632716 bytes, checksum: be05180f853f52d977f951f1a23dbfe1 (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A murcha-de-curtobacterium é umas principais doenças do feijoeiro e o uso de cultivares com níveis de resistência é fundamental para o manejo adequado da doença. A reação de 54 genótipos de feijoeiro comum a um isolado de Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) foi avaliada, assim como a colonização de cultivares e a obstrução de vasos de xilema primário da base de pecíolos de plantas inoculadas foram quantificadas. Ensaios conduzidos em casa-de-vegetação e laboratório evidenciaram resistência nas cultivares IAC Diplomata, IAC Alvorada, IAC Imperador, IPR Corujinha e IPR Tangará e nas linhagens P5-4-4-1 e C4-5-4-1-2. Uma menor velocidade de colonização por Cff também foi evidenciada nas cultivares resistentes (IAC Diplomata, IAC Carioca Tybatã e IAC Carioca Pyatã), assim como uma menor porcentagem de obstrução dos vasos de xilema primários na base de pecíolos, em comparação às cultivares suscetíveis (IAC Carioca e Pérola). Bacterial wilt is one of the main diseases of common beans and the use of cultivars with some level of resistance is fundamental for an adequate disease management. The reaction of 54 common bean genotypes to Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) was evaluated, and the colonization of cultivars and obstruction of primary xylem vessels at the petiole base were assessed. In greenhouse and laboratory tests, resistance was detected in the cultivars IAC Diplomata, IAC Alvorada, IAC Imperador, IPR Corujinha, and IPR Tangará, and in the lines P5-4-4-1 and C4-5-4-1-2. The Cff colonization rate was also slower in the resistant cultivars (IAC Diplomata, IAC Carioca Tybatã, and IAC Carioca Pyatã) and the percentage of obstruction of primary xylem vessels at the petiole base lower than in the susceptible cultivars (IAC Carioca and Pérola). Instituto Agronômico de Campinas Instituto Agronômico do Paraná UNESP Faculdade de Ciências Agronômicas Departamento de Proteção Vegetal

Made available in DSpace on 2016-04-01T18:46:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2014. Added 1 bitstream(s) on 2016-04-01T18:51:09Z : No. of bitstreams: 1 ISSN2221-1004-2014-04-04-208-211.pdf: 199093 bytes, checksum: 5dc189c6ef7505e7c5812ae14bfbd365 (MD5) Antioxidant capacities of seven species of Passiflora were evaluated through comparation of the free radical DPPH scavenging activity. The studied species included cultivated and traditionally used P. edulis, P. incarnata and P. alata and less common species P. coccinea, P. laurifolia, P. mucronata and P. gardneri. The experimental design was completely randomized with ANOVA and Tukey test as main statistical analyses. The results showed that species of Passiflora had variable antioxidant capacities, ranging from 28 to 95% of free radical DPPH scavenging activity. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agronômicas (FCA), Departamento de Horticultura, Botucatu, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências de Botucatu (IBB), Departamento de Química, Botucatu, SP, Brasil Instituto Agronômico de Campinas (IAC), Centro de Horticultura, Campinas, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agronômicas (FCA), Departamento de Horticultura, Botucatu, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências de Botucatu (IBB), Departamento de Química, Botucatu, SP, Brasil

Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early evaluation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasiliensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. Electronic supplementary material The online version of this article (doi:10.1007/s11032-014-0095-2) contains supplementary material, which is available to authorized users.

Os métodos de avaliação da aptidão agrícola tem como objetivo orientar o uso adequado das terras, porém seus resultados apresentam elevado grau de subjetividade devido a serem dependentes da experiência do usuário. Neste trabalho objetivou-se avaliar o uso de um algoritmo para interpretação de propriedades do solo e características da paisagem e compará-lo com a interpretação obtida por um grupo controle, constituído por seis especialistas. Foram utilizadas amostras de solo coletadas por tradagem ao lado dos perfis pedológicos da IX Reunião Brasileira de Correlação e Classificação de Solos, de modo a obter os dados de entrada do algoritmo, enquanto os avaliadores utilizaram os dados físicos, químicos e morfológicos dos perfis pedológicos. A avaliação do grupo controle não identificou as potencialidades para o uso dos solos na Amazônia de forma consistente, enquanto, o algoritmo mostrou-se promissor na avaliação da aptidão para Latossolo, Argissolos, Vertissolos e Luvissolo, no entanto, ainda requer revisões em algumas regras de interpretação para uma avaliação mais coerente da aptidão, principalmente, para o Espodossolo.

Made available in DSpace on 2022-04-28T18:59:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-12-01 The objective of the present work was to evaluate changes in the structure of coffee beans processed and dried in different ways. The experiment was conducted with two types of processing: dry and wet, and four drying methods: drying on ground and mechanical drying with air heated to 50/40 ° C, 60/40 ° C and 40/60 ° C, where the temperature was changed when the coffee beans reached 30% ± 2% (db), with supplementation of dehydrated to 11% ± 1% (wb). The mechanical drying system used consisted of three fixed bed dryers, which allows control of temperature and flow rate of drying. During the experiment, data were collected to assess the dynamics of drying.After treatment application, the coffee beans were subjected to studies in a scanning electron microscope (SEM). The observation of the coffee beans by SEM showed that the drying temperature of 50/40 ° C pulped coffees showed similar results to those dried in cafes, with a small contraction of the cells, without signs of rupture. It was observed that utilization of the drying temperature 40/60 ° C was the most damage caused to the structures of cells, regardless of the type of processing. It was found that the coffee prepared by dry showed higher destruction of the cellular components of the coffee pulped and, a longer exposure of coffee to the drying process. Universidade Federal de Lavras/UFLA Departamento de Engenharia/DEG, Cx. P. 3037, 37.200-000 - Lavras-MG Universidade Estadual Paulista 'Júlio de Mesquita Filho'/ UNESP Fazenda Lageado, Portaria I - Rua Jose Barbosa de Barros, no 1780, 18.610-307 - Botucatu-SP Instituto Agronômico de Campinas/IAC, Av. Barão de Itapura, 1481 -Cx. P. 28, 13012-970 - Campinas - SP Universidade Estadual Paulista 'Júlio de Mesquita Filho'/ UNESP Fazenda Lageado, Portaria I - Rua Jose Barbosa de Barros, no 1780, 18.610-307 - Botucatu-SP

Made available in DSpace on 2022-04-28T18:59:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-12-01 The objective of the present study was to evaluate the physiological and sensory quality of coffee beans processed and dried in different manners. The experiment was conducted with two types of processing - dry and wet processing, and four drying methods - drying in a drying yard, and mechanical drying with heated air at three alternating temperatures (50/40°C, 60/40°C and 40/60°C) where the temperature was changed when the coffee beans reached moisture content of 30% ± 2% (w.b.), with supplementation of drying until achieveing 11% ± 1% (w.b.). The mechanical drying system used consisted of three fixed bed dryers, which allows control of temperature and flow drying rate. After application of the treatments, the coffees were sampled according to the evaluation system proposed by the Specialty Coffee Association of America (SCAA). In addition to sensory analysis, analyses were made of the physical-chemical and physiological quality of the coffee beans. The physical-chemical and physiological analyses involved: fatty acid composition, leaching of potassium, electrical conductivity and germination. Interesting results were obtained. Coffee dried in the drying yard showed better sensory, physiological and physical-chemical results when compared with that dried in a dryer. Pulped coffee was more tolerant to drying than natural coffee, regardless of the way it was dried, showing better final quality of the product. Moreover, it may be observed that the increase in drying temperature in the final phase of the drying process leads to grain damage, which notably reduces beverage quality, confirming existing research. Universidade Estadual Paulista 'Júlio de Mesquita Filho'/UNESP Fazenda Lageado, Portaria I: Rua Jose Barbosa de Barros no 1780, 18.610-307 - Botucatu-SP Universidade Federal de Lavras/UFLA Departamento de Engenharia/DEG, Cx. P. 3037, 37.200-000 - Lavras - MG Instituto Agronômico de Campinas/IAC, Av. Barão de Itapura, 1481 - Cx. P. 28, 13012-970 - Campinas - SP Universidade Federal de Lavras/UFLA Departamento de Ciências Exatas/DEX, Cx. P. 3037, 37.200-000 - Lavras - MG Universidade Estadual Paulista 'Júlio de Mesquita Filho'/UNESP Fazenda Lageado, Portaria I: Rua Jose Barbosa de Barros no 1780, 18.610-307 - Botucatu-SP

The taxonomy of the genus Manihot is still uncertain and the genetic origin of cassava (M. esculenta Crantz) continues to be controversial. We studied the degree of genetic relatedness between cassava and two naturally occurring species (M. flabellifolia and M. peruviana) which are probably involved in the evolution of cassava, using RAPD and AFLP molecular markers. Thirty-three clonal accessions of cassava of known genetic diversity and 15 accessions of the wild species M. flabellifolia and M. peruviana were analyzed using 92 polymorphic RAPD bands and 73 polymorphic AFLP bands. The genetic markers were unable to differentiate the two wild species, which confirms their botanical similarity. Half of the total number of amplified bands were monomorphic in all of the genotypes evaluated. The mean genetic similarity (Jaccard) between cassava and the species M. flabellifolia/M. peruviana was 0.59. A grouping analysis (neighbor-joining method) with RAPD markers of cultivated cassava, M. flabellifolia/M. peruviana and the other wild species located the genotypes of cassava and M. flabellifolia/M. peruviana at one extremity and the three Mexican species (M. aesculifolia, M. michaelis and M. chlorostica) at the other. An intermediate position between these groups was occupied by two wild species (M. glaziovii and M. reptans) native to central and northeastern Brazil. These results are consistent with the hypothesis that the species M. flabellifolia and M. peruviana gave rise to the cultivated species.A taxonomia do gênero Manihot em grande parte não está resolvida e a origem genética da mandioca (M. esculenta Crantz) continua controvertida. Na tentativa de contribuir para elucidar sua história evolutiva, as relações de proximidade genética da mandioca com duas espécies selvagens que provavelmente participaram da sua história evolutiva, M. flabellifolia e M. peruviana, foram estudadas através de dois tipos de marcadores moleculares, os RAPDs e os AFLPs. Para tanto, foram empregados 33 acessos clonais de mandioca de reconhecida diversidade genética e 15 acessos das espécies selvagens M. flabellifolia e M. peruviana das regiões central e norte do Brasil, importantes centros de ocorrência natural destas espécies. Noventa e duas bandas polimórficas RAPD e 73 AFLP foram utilizadas para análise dos resultados. Ambos marcadores foram incapazes de diferenciar as duas espécies selvagens utilizadas, confirmando a grande semelhança botânica entre elas. Em relação à mandioca cultivada, os resultados revelaram grande proximidade genética entre estas e as espécies selvagens. Metade do total de bandas amplificadas apresentaram-se monomórficas entre todos os genótipos avaliados. O valor médio de similaridade genética (Jaccard) entre a mandioca e as espécies M. flabellifolia/M. peruviana é de 0.59. As relações de proximidade genética entre a mandioca e M. flabellifolia/M. peruviana foram confirmadas quando outras cinco espécies selvagens foram também incorporadas em relação ao polimorfismo gerado pelos RAPDs. A análise de agrupamento (neighbor-joining) realizada com genótipos de mandioca, de M. flabellifolia/M. peruviana e das demais espécies selvagens reuniu numa extremidade os genótipos de mandioca e M. flabellifolia/M. peruviana e na outra extremidade três espécies mexicanas (M. aesculifolia, M. michaelis e M. chlorostica). Entre estes dois grupos se posicionaram outras duas espécies selvagens cuja ocorrência natural é na região central e no nordeste brasileiro (M. glaziovii e M. reptans). Embora não conclusivos, os resultados apresentados são coerentes com a hipótese de que as espécies M. flabellifolia e M. peruviana poderiam ter originado a espécie cultivada. No entanto, outras espécies pouco estudadas (M. procumbens, M. fruticulosa, M. pentaphylla e M. pruinosa) foram recentemente citadas como geneticamente muito próximas da mandioca. Assim, um estudo abordando maior número de espécies e com marcadores mais apropriados, a exemplo dos microsatélites, merece ser feito.

In general, tropical soils present low concentrations of zinc (Zn), and the deficiency of Zn is recognized as a world nutritional problem for cereal production and human beings. Therefore, the main goal of this study was to assess the effects of different methods of Zn application on the quality of corn and sorghum grains grown in Oxisol. Two experiments were set up in the experimental area of UNESP (campus of Jaboticabal, Brazil). The following nine treatments were applied: three doses of Zn by banded application (seed furrows), three doses of Zn by incorporation into soil (0-20 cm depth), foliar application, seed application, and control (no Zn applied). The treatments were arranged in randomized blocks with four replicates. The contents of Zn, carbohydrates and proteins were determined for corn and sorghum grains. Regardless of the method, Zn application promoted higher contents of this micronutrient in corn and sorghum grains. The banded application method of Zn in soil promoted greater contents of total carbohydrates, starch and protein in both cultures. The incorporation of Zn into the soil method provided higher contents of soluble carbohydrates in both corn and sorghum grains. Los suelos tropicales, en general, tienen una baja concentración de zinc (Zn), y la deficiencia de este micronutriente es reconocida como un problema nutricional mundial para la producción de cereales y para los seres humanos. Así, el objetivo de este estudio fue evaluar los efectos de diferentes métodos de aplicación de Zn sobre la calidad del grano de maíz y sorgo. Para esto, se realizaron dos experimentos en el área experimental de FCAV/UNESP, Jaboticabal, Brasil. Se aplicaron nueve tratamientos: tres dosis de Zn aplicadas en forma localizada en los surcos de las semillas, tres dosis de Zn aplicadas en forma incorporada al suelo (0-20 cm de profundidad), una dosis aplicada en forma foliar, y el tratamiento control (sin aplicación de Zn); dispuestos en un diseño de bloques completos al azar con cuatro repeticiones. Las variables medidas fueron: contenido de Zn, proteínas y carbohidratos en los granos de maíz y sorgo. La adición de Zn, independientemente del modo, promovió mayores niveles de este micronutriente en los granos de maíz y sorgo. El modo de aplicación de Zn en el suelo de una forma localizada mostró mayores niveles de carbohidratos, almidón y proteína en las semillas de ambos cultivos. La aplicación de Zn en el suelo de modo incorporado resultó en altos niveles de carbohidratos solubles en los granos de maíz y sorgo.

Made available in DSpace on 2016-07-07T12:36:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2013. Added 1 bitstream(s) on 2016-07-07T12:45:08Z : No. of bitstreams: 1 ISSN1809-6093-2013-19-02-99-106.pdf: 1027808 bytes, checksum: 329e24391de79863f7c29a026996ad74 (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Passiflora quadrangularis L. é uma espécie originária do neotrópico, conhecida no Brasil como maracujá-melão devido ao tamanho e formato dos seus frutos. Tem sido amplamente disseminada nas regiões tropicais do mundo onde é produzida em pequena escala para comercialização dos frutos, o que ocorre bem próximo às regiões de cultivo, em função da dificuldade de conservação pós-colheita. As plantas são trepadeiras bastante vigorosas, com flores abundantes, grandes e perfumadas, o que amplia o seu valor e a torna particularmente interessante para caramanchões e cercas-vivas. O uso efetivo de passifloras no mercado de plantas ornamentais depende de estudos da fenologia floral. Neste trabalho foram estudadas algumas características da fenologia floral e o perfume das flores de P. quadrangularis, sob cultivo protegido. São apresentados os dados relativos ao período de floração, picos de florescimento e intensidade relativa das flores, bem como algumas características do perfume floral. Conclui-se que a espécie representa uma nova alternativa de cultivo para fins ornamentais, por apresentar múltiplas flores em antese simultânea, abundantes, grandes, fragrantes e coloridas, com longo período de floração no ano, além de frutos comestíveis, folhas exuberantes e medicinais que oferecem amplo sombreamento para áreas externas de lazer e/ou outras plantas cultivadas. Passiflora quadrangularis L. it’s a neotropical species, commonly known in Brazil as the melon passion fruit due to the size and format of its fruits. Currently, it has been widely disseminated throughout the tropics and it is commercially produced on a small scale, but mostly locally consumed because the fruits bruise easily when shipped. The plants of P. quadrangularis are vigorous climbers with abundant foliage and big beautiful scented flowers for which its use as ornamental plant seems particularly interesting. It has been stipulated that for the effective use of passion flowers in the ornamental plant market, studies need to be done on the flowering phenology. For this reason, we investigated some features regarding the flowering phenology and the composition of the scent produced by the flowers of P. quadrangularis cultivated at greenhouse. We present data concerning to the flowering period, blossom pick, relative blossom intensity and some characteristics of the flower scent. We conclude that P. quadrangularis represents a novel alternative as an ornamental plant in Brazil, because it’s abundant, big, scented, colorful, anthesis-simultaneous and flowers with long period of blossom. Also because it´s edible fruit and the medicinal properties of the exuberant foliage that could offer a nice shading for outdoor leisure areas and/or shade plants. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agronômicas (FCA), Departamento de Horticultura, Botucatu, SP, Brasil Instituto Agronômico de Campinas (IAC), Campinas, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agronômicas (FCA), Departamento de Horticultura, Botucatu, SP, Brasil