Search

Influenza virus PB1 protein plays the key role for the assembly and catalytic function of viral RNA polymerase. We showed that Ebp1 interacted with PB1 and interfered both the viral RNA polymerase function in vitro and the virus growth. The Ebp1 expression was induced by influenza virus infection using western blotting and quantitative real time PCR assay. However, the quantitative determination has been requested of the relationship between influenza virus growth and Ebp1 level. To analyze the relationship between virus growth and Ebp1 level, we developed the assay system of single cell. Using this system, we confirmed the tight correlation between the Ebp1 level and the influenza virus growth.

The degradation mechanism of 0.15 /spl mu/m GaAs PHEMTs subjected to three-temperature elevated lifetest (T/sub 1/=185/spl deg/C, T/sub 2/=200/spl deg/C, and T/sub 3/=215/spl deg/C ambient temperatures in N/sub 2/ atmosphere and stressed at Vds=5V/Ids=250 mA/mm) under RF-overdrive at 20 GHz was investigated. The results show that Pout degradation is due to Ids degradation induced by Ti gate metal interdiffusion into the AlGaAs Schottky barrier layer. However, /spl Delta/Imax, /spl Delta/Gmp, and Ti interdiffusion depth depend on the RF-drive levels. Accordingly, a distinct difference of reliability performance between DC (no RF-overdrive) and RF-overdrive lifetests was demonstrated. It has been found that both DC and RF-overdrive lifetests exhibit similar activation energy, which is approximately 1.65 eV. However, the mean-time-to-failure (MTTF) of RF-overdrive lifetest is inferior to that of DC lifetest. The difference is attributed to the higher electric field present in the RF-overdriven lifetest.

The Mosaic Editor described provides a number of features to facilitate the construction, refinement, and viewing of digital, side-scan sonar mosaics. The visually oriented, interactive Mosaic Editor allows geologists and data technicians to interact with the mosaic. Specific features include the ability to concurrently view and edit both bathymetry and intensity image mosaics, rapid visual feedback, automatic storage of the algorithms and parameters used in data processing, and an integrated data analysis workbench. Problems associated with digital mosaic editing are primarily those of performance and result from the immense volumes of data. A prototype solution is described, and a second generation solution is discussed. >

Administration of nerve growth factor (NGF) to the offspring of Sprague-Dawley rats transplacentally exposed to 50 mg/kg ethylnitrosourea on the 20th day of gestation resulted in a significant reduction of trigeminal and peripheral nerve neurinomas. Forty, 60, and 80 micrograms of NGF was administered in five s.c. doses, one dose on each of days 12-16, 90-94, and 210-214 postnatally. Of the 34 rats in the NGF-treated group, 11 animals were affected with trigeminal nerve neurinomas as compared to 18/34 in the NGF-untreated group (P less than 0.05). In the peripheral nerves (spinal cord nerve roots) there were five and 11 neurinomas, respectively, in each group of 34 rats. When the total numbers of neurinomas (trigeminal and peripheral nerves) between these groups were compared (16/34 versus 29/34), the significance of neurinoma reduction was P less than 0.01. Five trigeminal and two peripheral neurinomas in the NGF-untreated group were shown by immunohistochemical staining to contain nerve growth factor receptor protein, whereas none of the neurinomas in the NGF-treated group were positive for the receptor protein. The results obtained from this experiment lend support to the hypothesis that NGF has the capability to reduce the oncogenic consequences of ethylnitrosourea exposure perhaps by the process of maturation and/or differentiation of the transformed cells, and that this effect may depend upon the presence of receptor binding sites.