Ebp1 expression is induced by influenza virus infection

Influenza virus PB1 protein plays the key role for the assembly and catalytic function of viral RNA polymerase. We showed that Ebp1 interacted with PB1 and interfered both the viral RNA polymerase function in vitro and the virus growth. The Ebp1 expression was induced by influenza virus infection using western blotting and quantitative real time PCR assay. However, the quantitative determination has been requested of the relationship between influenza virus growth and Ebp1 level. To analyze the relationship between virus growth and Ebp1 level, we developed the assay system of single cell. Using this system, we confirmed the tight correlation between the Ebp1 level and the influenza virus growth.