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Background: Loss of brain-derived neurotrophic factor (BDNF)/TrkB (tropomyosin kinase receptor B) signaling accounts for brain and cardiac disorders. In neurons, β-adrenergic receptor stimulation enhances local BDNF expression. It is unclear if this occurs in a pathophysiological relevant manner in the heart, especially in the β-adrenergic receptor-desensitized postischemic myocardium. Nor is it fully understood whether and how TrkB agonists counter chronic postischemic left ventricle (LV) decompensation, a significant unmet clinical milestone. Methods: We conducted in vitro studies using neonatal rat and adult murine cardiomyocytes, SH-SY5Y neuronal cells, and umbilical vein endothelial cells. We assessed myocardial ischemia (MI) impact in wild type, β3AR knockout, or myocyte-selective BDNF knockout (myoBDNF KO) mice in vivo (via coronary ligation [MI]) or in isolated hearts with global ischemia-reperfusion (I/R). Results: In wild type hearts, BDNF levels rose early after MI ( Conclusions: BDNF loss underscores chronic postischemic heart failure. TrkB agonists can improve ischemic LV dysfunction via replenished myocardial BDNF content. Direct cardiac β3AR stimulation, or β-blockers (via upregulated β3AR), is another BDNF-based means to fend off chronic postischemic heart failure.

The synergetic effect between a nonionic silicone surfactant and an anionic hydrocarbon surfactant leads to strong nonconventional photoluminescence.

The author suggests using computer technology and data mining in intelligent engineering management to increase the management capacity of engineering projects and decrease the consumption of construction expenses. The emphasis is mostly on theoretical and analytical approaches to problems of practical concern for data mining, perhaps in combination with other conventional tools, and the associated applications to engineers and managers of various industrial sectors. This has immediate benefits for both academic and applied data mining researchers as well as research students. The author developed a BIM project management system based on Browser/Server architecture and Client/Server architecture in conjunction with BIM technology and the BIM 4D model. To execute complete project management of business management, real-time control, and decision support, the system must establish and use its business logic and data exchange link. Each module in the 4D construction dynamic management subsystem recognizes dynamic management of the construction process, and the working duration of each module is determined by calculating by using the unified engineering decomposition principle, rationalizing the relevant data through time parameters, recording the acquired management data and storing it in the BIM database, and connecting the BIM database bi-directionally through the web server through the system integration. The results showed that when the time is 20[Formula: see text]min, the throughput of the system is higher than [Formula: see text][Formula: see text]kb/s. The system can effectively improve the project management ability, reduce the construction cost and construction period of the project, and the system responds quickly.

Scholars propose a noise adding mechanism in local differential privacy to provide a strict privacy guarantee to federated learning. However, there are two problems. On the one hand, the noisy data is close to its original value with high probability due to the zero mean random noise, leading to the information exposure. On the other hand, a large variance of the noise makes the estimated average of the parameters bias. To overcome the difficulties, we propose a framework of none-zero mean noise adding mechanism in differential privacy in federated learning of deep networks based on fully homomorphic encryption and greedy average block Kaczmarz method. In the work, a none-zero mean adding noise to original data is determined locally according to the correlation between the local and global distributions. Using fully homomorphic encryption and greedy average block Kaczmarz method, a de-noising weighting aggregation strategy is derived without decryption of the mean to guarantee the privacy of user by a third party. Moreover, the weight is generated complete randomly so that it is difficult to obtain the mean. As the means are different among the clients and unpredictable for attackers, the variance of the noise can be small. Experiments show that the proposed method provides a higher level of accuracy than the non-privacy-preserving federated learning, while it guarantee privacy for multi-party machine learning tasks.

Li-Ping Yin,1 Fan Wang,2 Qian Li,2 Xiao-Ning Feng,3 Yan-Ling Li,2 Ling-Ling Li2 1Department of Oncology, The First Hospital Xingtai, Xingtai, Hebei, 054001, People’s Republic of China; 2Department of Tuberculosis, Affiliated Hospital of Hebei University, Baoding, Hebei, 071000, People’s Republic of China; 3Dosing Center, Affiliated Hospital of Hebei University, Baoding, Hebei, 071000, People’s Republic of ChinaCorrespondence: Ling-Ling Li, Department of Tuberculosis, Affiliated Hospital of Hebei University, No. 648 of Dongfeng East Road, Lianchi District, Baoding, Hebei, 071000, People’s Republic of China, Email lin1697g@126.comBackground: Patient safety is an important aspect of healthcare delivery and is critical to healthcare quality. An assessment of the attitudes of nursing staff in infectious diseases wards towards patient safety may identify deficiencies and allow for the development of educational programmes to train nursing staff to participate in good patient safety practices.Objective: To explore the current situation and influencing factors on nurses’ attitudes towards patient safety in infectious diseases wards.Methods: This cross-sectional study enrolled 446 nurses from infectious diseases wards in eight hospitals in the Hebei Province to participate in an electronic questionnaire survey from October to December 2020. The Chinese version of the safety attitude questionnaire was used to gather the opinions of these participants, and SPSS 22.0 statistical software was used to analyse the data.Results: The average score of safety attitudes towards patients was 3.59 ± 0.30. The scores for each dimension, from high to low, were as follows: management perception: 3.77 ± 0.42 points, pressure perception: 3.77 ± 0.42 points, safety atmosphere: 3.57 ± 0.43 points, job satisfaction: 3.57 ± 0.43 points, teamwork: 3.55 ± 0.50 points and working conditions: 3.50 ± 0.45 points. The results of the multiple stepwise regression analysis showed that the influential factors on nurses’ attitudes towards patient safety in infectious diseases wards were as follows: night shift working (β = 11.885, P = 0.000), years of nursing experience (β = 2.862, P = 0.001), education level (β = 4.462, P = 0.001) and marital status (β = 3.871, P = 1.002), which together explained 33.5% of the total variance.Conclusion: Nurses’ attitudes towards patient safety in infectious diseases wards were moderately high. Night shift work, years of nursing experience, education level and marital status affected nurses’ attitudes towards patient safety. Managers should focus on these groups of nurses and improve their working conditions and job satisfaction to further enhance patient safety.Keywords: infectious diseases wards, the nurse, patient safety attitude, influencing factors

We have designed a single-particle detection assay for quantifying Hg2+ by DFM-correlated Raman spectroscopy. A tetrahedral DNA-directed core-satellite nanostructure is used as the SERS probe.

Wormlike micelles were constructed by mixing C14DMAO and DPA in water. When Eu3+ or Tb3+ was introduced into the micellar solution, complexes formed in situ, leading to the formation of luminescent fluids with high stability.

Objective Compared with other regions in the world, the transmission characteristics of the COVID-19 epidemic in Africa are more obvious, has a unique transmission mode in this region; At the same time, the data related to the COVID-19 epidemic in Africa is characterized by low data quality and incomplete data coverage, which makes the prediction method of COVID-19 epidemic suitable for other regions unable to achieve good results in Africa. In order to solve the above problems, this paper proposes a prediction method that nests the in-depth learning method in the mechanism model. From the experimental results, it can better solve the above problems and better adapt to the transmission characteristics of the COVID-19 epidemic in African countries. Methods Based on the SIRV model, the COVID-19 transmission rate and trend from September 2021 to January 2022 of the top 15 African countries (South Africa, Morocco, Tunisia, Libya, Egypt, Ethiopia, Kenya, Zambia, Algeria, Botswana, Nigeria, Zimbabwe, Mozambique, Uganda, and Ghana) in the accumulative number of COVID-19 confirmed cases was fitted by using the data from Worldometer. Non-autoregressive (NAR), Long-short term memory (LSTM), Autoregressive integrated moving average (ARIMA) models, Gaussian and polynomial functions were used to predict the transmission rate β in the next 7, 14, and 21 days. Then, the predicted transmission rate βs were substituted into the SIRV model to predict the number of the COVID-19 active cases. The error analysis was conducted using root-mean-square error (RMSE) and mean absolute percentage error (MAPE). Results The fitting curves of the 7, 14, and 21 days were consistent with and higher than the original curves of daily active cases (DAC). The MAPE between the fitted and original 7-day DAC was only 1.15% and increased with the longer of predict days. Both the predicted β and DAC of the next 7, 14, and 21 days by NAR and LSTM nested models were closer to the real ones than other three ones. The minimum RMSEs for the predicted number of COVID-19 active cases in the next 7, 14, and 21 days were 12,974, 14,152, and 12,211 people, respectively when the order of magnitude for was 106, with the minimum MAPE being 1.79%, 1.97%, and 1.64%, respectively. Conclusion Nesting the SIRV model with NAR, LSTM, ARIMA methods etc. through functionalizing β respectively could obtain more accurate fitting and predicting results than these models/methods alone for the number of confirmed COVID-19 cases in Africa in which nesting with NAR had the highest accuracy for the 14-day and 21-day predictions. The nested model was of high significance for early understanding of the COVID-19 disease burden and preparedness for the response.

The concept design evaluation phase of the new product launch is extremely important. However, current evaluation information relies mainly on the a priori knowledge of decision makers and is subjective and ambiguous. For this reason, a conceptual design solution decision model based on Pythagorean fuzzy sets in a big data environment is proposed. Firstly, we use the ability of big data to mine and analyze information to construct a new standard for product concept design evaluation in the big data environment. Secondly, the Pythagorean fuzzy set (PFS), Analytic Hierarchy Process (AHP), and Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) are integrated into a decision model. AHP, extended by the Pythagorean fuzzy set, is used to determine the weights of new conceptual design criteria in a big data environment. The Pythagorean fuzzy TOPSIS is used to prioritize alternative conceptual design solutions. The feasibility of the approach is proven with a practical case, the generalizability of the method is confirmed with two descriptive digital cases, and the reliability, validity, and superiority of the process are demonstrated with sensitivity analysis, comparative analysis, and computational complexity analysis.

The immune imbalance caused by excessive inflammatory reactions is the primary cause of sepsis. Macrophages with M1 and M2 polarization states are important immune cells that regulate the balance of the inflammatory response in sepsis. Encouraging the conversion of macrophages from the M1 to the M2 type is an important strategy for relieving sepsis. Here, we demonstrated the upregulation of vascular endothelial growth factor A (VEGFA) in a mouse model of sepsis. Then, siRNA technology was applied to inhibit the expression of VEGFA in macrophages. Flow cytometry and RT‒qPCR results showed that low expression of VEGFA inhibited LPS-induced M1 polarization of macrophages. Decreased VEGFA was also proven to lower TNF-α, IL-1β, and IL-6 secretion by LPS-induced macrophages. In addition, the effects of knocking down VEGFA on the energy metabolism pattern of macrophages were investigated by glycolysis pressure tests and mitochondrial pressure tests, and VEGFA knockdown reversed the induction of glycolysis in macrophages by LPS. The mitochondrial content and ATP content results also confirmed this finding. After the tail vein of septic mice was injected with macrophages transfected with si-VEGFA, the liver and kidney damage and the pathological conditions of the lung were alleviated. The secretion of TNF-α and IL-6 was decreased, while IL-10 was increased in their serum. Immunohistochemical staining revealed decreased expression of CD86 and increased expression of CD206 in the si-VEGFA group. This study demonstrates that decreased VEGFA inhibits glycolysis and thus inhibits LPS-induced M1 polarization of macrophages, ultimately relieving sepsis.

Carbon dots (CDs) have developed into an important class of nanomaterials that have attracted increasing attention during the past decades. Despite numerous types of CDs reported to date, research on their self-assembly is still limited. Herein, we report for the first time the self-assembly of CDs in water, which show concentration-dependent aggregation behavior. The CDs used have a structural motif of a fully carbonized core surrounded by a highly condensed, polymeric network, to which triethylene glycol monomethyl ether (TGME) chains are grafted. When dissolved in water, they show a low critical aggregation concentration (cac) of 0.07 mg mL

Prevention of African swine fever, a disease caused by African swine fever virus (ASFV), requires maintenance of high biosecurity standards, which principally relies on disinfection. Finding the perfect disinfectant against ASFV is difficult because of the lack of relevant data. Therefore, we aimed to find the most effective disinfectant and to optimise its concentration as well as contact time to confirm the viricidal effect against ASFV in vitro. We evaluated the viricidal activity of three concentrations each of six common disinfectants against ASFV using immersion disinfection assay (IDA) and spray disinfection assay (SDA); the concentrations of these disinfectants at which complete viral inactivation occurred were almost same as the manufacturer-recommended concentrations, but the exposure times for viral inactivation are different. The following disinfectants (assay: concentration, exposure time) showed complete inactivation: iodine and acid mixed solution (IDA/SDA: 0.5%, 10 min); compound potassium peroxymonosulfate (IDA: 0.25%, 30 min; SDA: 0.25%, 60 min); citric acid (IDA: 0.25%, 60 min; SDA: 0.5%, 60 min); sodium dichloroisocyanurate (IDA: 0.125%, 60 min; SDA: 0.25%, 60 min); and glutaral ang deciquam (IDA/SDA: 0.2%, 60 min); and deciquam (IDA/SDA: 0.5%, 60 min). However, in the presence of organic material contamination, disinfectants did not show a marked inactivation effect. Therefore, disinfection procedures should be performed in two steps: thorough mechanical cleaning followed by application of disinfectant. In conclusion, all the tested disinfectants can inactivate ASFV; these can be used as alternative disinfectants to enhance biosecurity.

Background The mortality rate among patients with nasopharyngeal carcinoma (NPC) has improved significantly with the advent of chemoradiotherapy strategies. However, distant metastasis remains problematic. Tumor-specific reactivity in cancer patients has been detected exclusively in CD39+ T cells, particularly in CD39+CD103+ T cells. Circulating cancer-specific T cells are important for protecting against metastasis. This study aimed to evaluate the predictive value of circulating CD39+CD8+ T cells for metastasis in patients with NPC. Methods We performed a cross-sectional, longitudinal study of 55 patients with newly diagnosed NPC of stage III–IVa. All patients were initially treated with standard combined chemoradiotherapy. Blood samples were obtained from 24 patients before and at 1 month and 6 months after treatment. T cell expression of CD39 and CD103, together with the markers of T cell exhaustion programmed death-1 (PD-1)/T cell immunoglobulin and mucin domain-containing protein 3 (Tim-3) and markers of cell differentiation CD27/CC-chemokine receptor 7/CD45RA, was examined by flow cytometry. The Wilcoxon rank-sum test analysis was used to analyze the differences between two groups. Kaplan-Meier analysis was used for analysis of progression-free survival (PFS). Results The expression of circulating CD39+CD8+ and CD39+CD103+ CD8+ T cells was significantly higher in patients without distant metastasis (CD39+CD8+: 6.52% [1.24%, 12.58%] vs. 2.41% [0.58%, 5.31%], Z=−2.073, P=0.038 and CD39+CD103+CD8+: 0.72% [0.26%, 2.05%] vs. 0.26% [0.12%, 0.64%], Z=−2.313, P = 0.021). Most CD39+ T cells did not express PD-1 or Tim-3. Patients with high expression of CD39+CD103+CD8+ T cells had better PFS than patients with low expression (log rank value = 4.854, P = 0.028). CD39+CD8+ T cells were significantly elevated at 1-month post-treatment (10.02% [0.98%, 17.42%] vs. 5.91% [0.61%, 10.23%], Z = −2.943, P = 0.003). The percentage of advanced differentiated CD8+ T cells also increased at 1-month post-treatment compared with pre-treatment (33.10% [21.60%, 43.05%] vs. 21.00% [11.65%, 43.00%], Z = −2.155, P = 0.031). There was a significant correlation between elevated CD39+CD8+ T cells and increased effector memory T cells (intermediate stage: r = 0.469, P = 0.031; advanced stage: r = 0.508, P = 0.019). Conclusions CD39+CD8+ circulating T cells have preserved effector function, contributing to an improved prognosis and a reduced risk of metastasis among NPC patients. These cells may thus be a useful predictive marker for a better prognosis in patients with NPC.

Construction of controllable chiroptical supramolecular luminescence systems is of great significance for developing intelligent chiral luminescence materials with precise and effective regulation and understanding chirality-switching phenomena in biological systems, which has attracted extensive attention. Because chiral metal nanoclusters (NCs) can provide facilities for the study of nanoscale chiral effects, in this study, we select chiral glutathione-stabilized copper NCs (G-SH-Cu NCs) to construct a supramolecular luminescent hydrogel with achiral branched polyethyleneimine (PEI) and polyoxometalates [Na

Background Anti-NMDA receptor encephalitis is an autoimmune disease characterized by complex neuropsychiatric syndrome and cerebrospinal fluid (CSF) NMDAR antibodies. Triggering receptor expressed on myeloid cells 2 (TREM2) has been reported to be associated with inflammation of the CNS. Matrix metalloproteinase-9 (MMP9) and the cluster of differentiation (CD44) were measured to evaluate the blood-brain barrier (BBB) permeability of anti-NMDAR encephalitis. The roles of the microglia activation and disruption of BBB in anti-NMDAR encephalitis are not well known. Findings: In this work, we detected the increased expression level of CSF sTREM2, CSF and serum CD44, and serum MMP9 in anti-NMDAR encephalitis patients, compared with control groups. CSF sTREM2 levels were positively related to both the CSF CD44 levels (r = 0.702, p

Yale Yang,1 Wuling Zhong,1 Yali Zhang,1 Yalan Cheng,1 Hejin Lai,1 Huimin Yu,1 Ning Feng,1 Yumo Han,2 Rui Huang,1 Qiwei Zhai1,2 1CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, People’s Republic of China; 2School of Life Science and Technology, ShanghaiTech University, Shanghai, People’s Republic of ChinaCorrespondence: Qiwei Zhai, Shanghai, 200031, People’s Republic of China, Tel +86 21 5492 0903, Fax +86 21 5492 0291, Email qwzhai@sibs.ac.cnBackground: Sustained inflammation is implicated in a variety of pathological conditions like infection, obesity and type 2 diabetes. Lipid metabolism is crucial to support immune response during infection of bacteria. However, how sustained inflammation affects lipid metabolism, especially in white adipose tissue remains largely unknown.Methods: Sustained inflammation was induced by daily injection of Lipopolysaccharide (LPS). Tlr4 knockout mice were used to study the mechanism. Inflammation and lipid metabolism were evaluated by quantitative PCR, white blood cell counting, nuclear magnetic resonance, fat cell size quantification, lipolysis and fatty acid uptake assays, respiratory exchange ratio, and energy expenditure.Results: Here, we found that sustained inflammation leads to fat loss in mice with a quick loss and gradual increase manner. Moreover, LPS injection leads to inflammation, anorexia, decreased lipid anabolism, and increased lipid catabolism. Mechanically, we show that LPS induces fat loss, inflammation, anorexia, and alteration of lipid metabolism mainly dependent on Tlr4. Interestingly, sustained inflammation induces less fat loss, especially in epididymal white adipose tissue, than pair-feeding, and pair-feeding has no significant effect on inflammation and leads to less fatty acid uptake, more lipid catabolism and energy expenditure than LPS injection. In addition, we demonstrate that short-term sustained inflammation leads to relative long-term tolerance for LPS-induced anorexia, inflammation and altered lipid metabolism.Conclusion: These findings demonstrate that sustained inflammation induced by LPS leads to tolerable anorexia and fat loss via Tlr4 in mice, and provide new insights into the effect of sustained inflammation on lipid metabolism and subsequent tolerance.Keywords: sustained inflammation, lipopolysaccharide, white adipose tissue, lipid metabolism, tolerance

In recent years, chemodynamic therapy (CDT) has gained increasing interest in cancer treatment. In contrast to photodynamic therapy and sonodynamic therapy, extrinsic excitations such as laser or ultrasound are not required in CDT. As a result, the CDT performance is not limited by the penetration depth of the external irritation. However, CDT relies heavily on hydrogen peroxide (H2O2) in the tumour microenvironment (TME). Insufficient H2O2 in the TME limits the CDT performance, and the most reported methods to produce H2O2 in the TME are dependent on oxygen supply, which is restricted by the hypoxic TME. In this study, H2O2 self-providing copper nanodots were proposed, and the drug doxorubicin (DOX) was successfully loaded to construct DOX-nanodots. Our results showed that the nanodots produced H2O2 in the weakly acidic TME due to the peroxo group and further generated the most active hydroxyl radical (OH) through the Fenton-like reaction. This process was pH-dependent and did not occur in a neutral environment. In addition to OH, the nanodots also produced singlet oxygen (1O2) and superoxide anions (O2-) in the cancer cells. The copper nanodots performed promising CDT against breast cancer in vitro and in vivo, with enhanced cell apoptosis and decreased cell proliferation. The combination of chemotherapy and CDT using DOX-nanodots further improved the therapeutic effects. The treatments showed good biocompatibility with no obvious toxicity in major tissues, possibly due to the specific OH generation in the weakly acidic TME. In summary, the H2O2 self-providing copper nanodots in combination with DOX showed promising cancer-curing effects due to the oxygen-independent and tumour-specific production of reactive oxygen species and the cooperation of chemotherapy.

It was found in previous observational studies that the quasi-biennial oscillation (QBO) can modulate the teleconnection over the Atlantic basin related to the Madden–Julian Oscillation (MJO). In t...

The prediction skill of the North Atlantic Oscillation (NAO) in boreal winter is assessed in the operational models of the WCRP/WWRP Subseasonal-to-Seasonal (S2S) prediction project. Model performance in representing the contribution of different processes to the NAO forecast skill is evaluated. The S2S models with relatively higher stratospheric vertical resolutions (high-top models) are in general more skillful in predicting the NAO than those models with relatively lower stratospheric resolutions (low-top models). Comparison of skill is made between different groups of forecasts based on initial condition characteristics: phase and amplitude of the NAO, easterly and westerly phases of the quasi-biennial oscillation (QBO), warm and cold phases of ENSO, and phase and amplitude of the Madden-Julia Oscillation (MJO). The forecasts with a strong NAO in the initial condition are more skillful than with a weak NAO. Those with negative NAO tend to have more skillful predictions than positive NAO. Comparisons of NAO skill between forecasts during easterly and westerly QBO and between warm and cold ENSO show no consistent difference for the S2S models. Forecasts with strong initial MJO tend to be more skillful in the NAO prediction than weak MJO. Among the eight phases of MJO in the initial condition, phases 3-4 and phase 7 have better NAO forecast skills compared with the other phases.The results of this study have implications for improving our understanding of sources of predictability of the NAO. The situation dependence of the NAO prediction skill is likely useful in identifying “ windows of opportunity” for subseasonal to seasonal predictions.

BackgroundIntracranial germ cell tumors (GCTs) are a relatively rare malignancy in clinical practice. Natural regression of this tumor is also uncommon. We describe a rare case of an intracranial GCT in the thalamus of an adult that showed spontaneous regression and recurrence after steroid therapy.Case descriptionA 38-year-old male patient's MRI of the head suggested space-occupying masses in the left thalamus and midbrain. MRI examination revealed demyelination or granulomatous lesions. After high dose steroid treatment, the symptoms improved. The lesions were significantly reduced on repeat MRI, and oral steroid therapy was continued after discharge. The patient's symptoms deteriorated 1 month prior to a re-examination with head MRI, which revealed that the mass within the intracranial space was larger than on the previous image. He revisited the Department of Neurosurgery of our hospital and underwent left thalamic/pontine mass resection on October 16, 2019, and the pathological results showed that the tumor was a GCT.ConclusionIntracranial GCTs are rare in the adult thalamus but should be considered in the differential diagnosis. The intracranial GCT regression seen in this case may be a short-lived phenomenon arising from complex immune responses caused by the intervention.

Objective To observe the effect of the lower limb rehabilitation system on the function of the lower limbs of patients after total hip replacement, and to explore the advantages and clinical application value of the lower limb rehabilitation system. Methods Sixty patients admitted to the orthopaedic ward of the Affiliated Hospital of Chengdu University from January 2021 to June 2021 who underwent total hip arthroplasty for different etiologies were analyzed.The patients were divided into a conventional rehabilitation group (Group A) and a lower limb rehabilitation system rehabilitation group (Group B) using a random number table, with 30 cases in each group.Fugl-Meyer scores were collected at the follow-up visits after 8, 12 and 16 weeks of rehabilitation treatment respectively. Holden Functional Ambulation Classification (FAC) data were collected at the follow-up visits after 16 weeks of rehabilitation treatment for evaluation and analysis. Peak moment values, single foot support time, step frequency and 10m maximum walking speed were collected at week 16 using the lower limb rehabilitation system. Results There was no statistical difference between the general data of the two groups and they were comparable. After 16 weeks of training, the Fugl-Meyer scores of limb function increased in both groups as the training time increased; the Fugl-Meyer scores of limb function were higher in Group B than in Group A for the same training time (P 0.05). Conclusion The Lower Limb Rehabilitation System combined with conventional rehabilitation training can significantly improve the lower limb motor function, balance function and walking ability of patients after total hip arthroplasty in the early postoperative period, enabling patients to recover better and faster, and has greater clinical application value.

BackgroundAberrant cytokeratin 7 expression by hepatocytes (CK7+Hs) is the hallmark characteristic of cholestasis diseases, especially in ductopenia diseases such as primary biliary cholangitis (PBC). This study attempted to evaluate the differences and relationships between the clinical and histological features of aberrant cytokeratin 7 (CK7) expression by hepatocytes in PBC patients.MethodsThe clinicopathological data of patients diagnosed with PBC at the Second Hospital of Nanjing between January 2016 and September 2018 were analysed with SPSS 20.0.ResultsEighty-nine PBC patients who underwent liver biopsy were enrolled in this study, and 15, 29 and 45 patients had aberrant CK7 expression by hepatocytes (CK7+Hs (2 +), CK7+Hs (1 +), and CK7−Hs, respectively). There were significant differences in TB, DB, ALP, TA, IgM, interface activity, and ductopenia grade between patients with CK7−Hs and CK7+Hs (2 +) (P +Hs (2 +) and CK7+Hs (1 +) according to sex (P +Hs (2 +) and CK7+Hs (1 +) into CK7+Hs, we found significant differences in AMA, AMA-M2, anti-gp210, TB, DB, ALP, TA, IgM, fibrosis, and ductopenia grade between CK7+Hs and CK7−Hs (P +Hs according to anti-gp210, ductopenia grade, and interface activity were 6.413 (95% CI 1.363–30.162), 4.145 (95% CI 1.898–9.052) and 3.247 (95% CI 1.556–6.775), respectively (P +Hs (2 + , 1 + , 0) wasr = 0.359 (P = 0.001) andr = 0.396 (P ConclusionCK7+Hs serves as a cholestasis index of PBC and are associated with the ductopenia grade and interface activity. Aberrant cytokeratin 7 expression by hepatocytes can predict the ductopenia grade in primary biliary cholangitis.

The main framework of green construction management based on building information modeling (BIM) is constructed, and the related theories of BIM and green buildings are researched in more detail. The index of green construction is evaluated using the fuzzy evaluation method. The technical measures, management methods, field experience, and evaluation methods included in the green construction process are introduced in detail. Finally, effective measures to promote green construction management in China are proposed. This method is based on the specific technical measures and targeted management methods adopted in the green construction process. At the same time, the specific measures of construction project management in the era of big data are analyzed. It includes the collection and processing of data, the establishment of basic data models, the intelligent technology of big data clusters, and the application of blockchain big data. The purpose of the research is not only to effectively promote the development process of green construction in China, but also to reduce the waste of resources and environmental damage in the construction projects. At the same time, in the process of solving China’s environmental protection, building construction must follow the guiding suggestions put forward under the green construction. This has an important reference value for promoting China’s green construction work and has certain construction significance for realizing the sustainable development of the construction industry.

Pneumatic muscle fibers (PMFs) are a type of pneumatic artificial muscles (PAMs) with millimeter-scale diameter, which show great power to be a soft actuator under injecting the compressed fluid in...

Acute pancreatitis (AP) is an inflammatory disease with rapid progression. In severe cases, it can cause systemic inflammatory response syndrome (SIRS), multiple organ failure (POF) and even death. The study aimed to investigate the diagnostic value of C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6) and lactate dehydrogenase (LDH) in patients with severe AP.AP patients (n = 153) divided into mild AP patients (n = 81) and severe AP patients (n = 72) were selected from June 2014 to June 2016. The demographic information (age, sex) and the hematological parameters (WBC, PLT, CRP, PCT, IL-6, LDH and so on) were analyzed.Significant differences were found out of CRP, PCT, IL-6 and LDH values between AP patients and controls (P 0.05), even those results had significant difference between MAP group and SAP group (P 0.05). In SAP group, the cut-off values of CRP, PCT, IL-6 and LDH were 16.62, 2.29, 16.66, 273.04; sensitivity 55.6%, 77.8%, 80.2%, 82.7%; specificity 73%, 94%, 85%, 96% and AUC 0.637, 0.929, 0.886, 0.919, respectively. The AUC of combined detection of CRP, PCT, IL-6 and LDH was 0.989 (95%CI).The combined detection of CRP, PCT, IL-6 and LDH has a high diagnostic value for judging the severity of AP.

Background The prognosis of prostate cancer (PCa) is related to tumor metastasis, among which 80% were bone metastasis. In this study, we investigated the correlation between diverse clinical factors and bone metastasis in PCa patients and identified potential biomarkers of bone metastasis in PCa patients. Methods The clinical data of 150 PCa patients were reviewed consecutively from January 2015 to March 2020 in this study. The relationships between clinical characteristics, serum biomarkers and bone metastasis in PCa patients were analyzed, respectively. Multivariate logistic regression analysis was applied to identify potential markers of bone metastasis in prostate cancer. Receiver operating characteristic (ROC) curve was used to explore the diagnostic values of potential biomarkers. Results Compared with the PCa patients without bone metastasis, the serum levels of CA-125, T-PSA, F-PSA, CYFRA21-1 and ProGRP were significantly elevated in PCa patients with bone metastasis. Multivariate logistic regression analysis showed that T-PSA (OR 1.014, P = 0.021), F-PSA (OR 1.124, P = 0.016) and Pro-gastrin-releasing peptide (ProGRP) (OR 1.057, P = 0.026) were significantly associated with the bone metastasis of PCa patients. ROC curves indicated that T-PSA, F-PSA and ProGRP could effectively discriminate bone metastasis from non-bone metastasis PCa patients, and the AUCs (area under the curves) were 0.885, 0.919 and 0.752, respectively. According to the Youden index, the cut-off values of T-PSA, F-PSA and ProGRP were defined as 56.50 ng/ml, 6.96 ng/ml and 31.60 pg/ml, respectively. T-PSA, F-PSA and ProGRP produced a sensitivity of 78.30%, 81.70% and 61.70%, a specificity of 93.30%, 88.90% and 82.20%, respectively. The AUC for the combination of T-PSA, F-PSA with ProGRP was 0.941 with 90.00% sensitivity, much better than that of any single biomarker or two biomarkers combinate. Conclusions Serum ProGRP might be a potential tumor marker of bone metastasis in prostate cancer, which may contribute to the early diagnosis of bone metastasis when used alone or in combination with other commonly used biomarkers.

BACKGROUND: Indoor environment is complex, with many factors potentially interacting with each other to affect health. However, previous studies have usually focused on effect of a single factor. Assessment of the combined effects of multiple factors can help with understanding the overall health risk. METHODS: A cross-sectional study was conducted among 2,306 school children in Guangzhou and Shenzhen. Questionnaire data on respiratory symptoms and diseases were collected along with sociodemographic and residential environmental information. A subset of children (N=987) were measured for their lung function. A random forest algorithm was applied to screen the top-ranked indoor environmental exposure variables and to form a composite index for cumulative risk of indoor pollution (CRIP). Logistic regressions were conducted to analyze the independent effect of single indoor environmental risk factors and the combined effect of CRIP on children’s respiratory health. Multiple linear regressions were used to examine the independent and combined effects of indoor environmental exposure on lung function. RESULTS: We found that home dampness and molds as well as environmental tobacco smoke (ETS) were significantly and independently associated with increased prevalence of children’s respiratory symptoms and diseases and with reduced lung function. A higher CRIP level was significantly associated with increased risk of cough with cold (OR =1.37, 95% CI: 1.05–1.79) and wheeze (OR =2.71, 95% CI: 1.16–6.34). A higher CRIP level was also associated with reduced lung function measured as FVC, FEV(1), PEF, FEF(25%), FEF(25–75%) and VC. CONCLUSIONS: In children living in the subtropical region of the Pearl River Delta, home dampness and the presence of mold as well as ETS were individual risk factors for children’s respiratory health. The composite CRIP index was associated with respiratory symptoms and lung function, suggesting the utility of this index for predicting the combined effects of multiple risk factors.

Hydrogen peroxide (H2O2), as a signaling molecule, plays a vital role in a wide variety of signaling transduction processes, aging, and diseases. However, the excessive production of H2O2 causes various diseases. Herein, we develop a novel method for H2O2 detection in live cells via dark-field scattering spectroscopy with gold triangular nanoprisms (AuTNPs) as probes. The corners of AuTNPs would be gradually oxidatively etched by the strong coordination of Br• which is generated by enzymatic reactions in the presence of horseradish peroxidase (HRP), bromide ion and trace hydrogen peroxide. Benefitting from the morphological change, the single AuTNP based plasmonic nanoprobe shows notable blueshifts and scattering color changes which could be real-time monitored under the dark-field microscopy. The peak position in the scattering spectra of individual AuTNP blueshifts linearly with the increase of H2O2 concentration, and exhibits high sensitivity to H2O2 in a large range from 2.5 to 100 µM with a low detection limit (LOD) of 0.74 µM. Moreover, the experimental results were supported by the simulated results via the finite-difference time-domain (FDTD) method. The nanoprobes have been further used for intracellular H2O2 detection in live cells. Besides, the etching of AuTNP also provides an alternative method to design novel plasmonic logic chips and write-once plasmonic memories.

The complex curved contours of the zygomatic regions are difficult to analyze. Therefore, a better evaluation medium must be developed. We aimed to examine and summarize the morphological characteristics of the zygomatic region by using a moiré pattern map and computer algorithm. In this cross-sectional study, we collected three-dimensional images of the facial contours of 251 Han Chinese youth and established a morphological moiré map database. Clustering analysis using a computer algorithm was applied to obtain the zygomatic morphologies for classification. Aesthetic evaluation was performed to summarize the characteristics of the zygomatic types and provide reference for the preoperative morphological design of the midface. Zygomatic regions were morphologically classified into five types. Each type had its typical feature in the moiré pattern map. The moiré stripes in the left zygomatic region formed an “Ω” shape outward and downward in type 1, and they tended to be diagonal like “\\\” in type 2, smoothly curved like “)))” in type 3, vertical like “|||” in type 4, and diagonal like “///” in type 5. The aesthetic evaluation outcome indicated that the integrally flat zygoma (type 4) was more aesthetically pleasing among males, and the integrally prominent zygoma (type 3) was more aesthetically pleasing among females. Five morphological contour types of the zygoma were classified among the Chinese Han males and females based on the simulated moiré pattern. This morphological classification would aid in preparing a guide for clinical diagnosis and surgical planning.

In this paper, three kinds of aliphatic alcohol polyoxyethylene ethers (AEO) with different molecular weight distribution were used as raw materials, and aliphatic alcohol polyoxyethylene ether phosphate (AEP) was obtained by P2O5 process. FT-IR and 31P-NMR were used to characterize the structure of the product, confirming the formation of the product. HPLC-ESI was used to probe the molecular weight distribution of the sample. The surface activity, wetting properties and application properties of the samples were measured. Wetting and permeability were found to increase with decreasing EO addition, and N-AE7P is optimal, which is related to the spatial structure of the molecule. The surface activity, washing performance and foaming properties of C-AE9P are better as that of the other investigated compounds because of the synergistic effect between the complex components.

Background Youth friendly services (YFS) was established in pilot areas in China. This study aimed to explore the promoting level on the awareness and utilization of YFS after the implementing of a multi-sectoral cooperation mechanism (MSCM) supported by social network theory (SNT) among multiple sectors related to young people reproductive health (YRH) closely. Methods A cross-sectional study with two separate self-administered questionnaire surveys was conducted before and after the implementing of a MSCM supported by SNT in both in-school and out-school unmarried young people aged 12–24 year-old in pilot areas in China. Both pre- and post- implementation surveys were conducted between December, 2008 and January, 2009, and between October, 2010 and January, 2011 respectively. The collected categorical data about the awareness on YFS in young people was described in percentage (%). χ 2 test was used to compare the differences between interventional and control areas, pre and after intervention, and changes in investigated areas after the intervention respectively. Binary logistic regression was used to analyze interventional effects after adjusting gender, in-school or out-school, and other factors. Significance level α was 0.05. Results The percentages of young people in interventional areas who could receive YRH education including that about YFS in schools, working sites and communities increased (OR = 15.485, 6.166, 3.723; 95% CI: 2.939~4.715, 4.014~9.473, 11.421~20.994 respectively) statistically significantly (P OR = 9.325, 11.244; 95% CI: 7.433~11.699, 8.780~14.399 respectively), and knowledge rates on YFS manner and contents also increased (OR = 14.830, 8.676; 95% CI: 9.728~22.607, 5.175~14.548 respectively) statistically significantly (P P OR = 6.394, 95% CI: 2.789~14.655) statistically significantly (P Conclusions SNT is a helpful tool to facilitate the construction of an effective multi-sectoral cooperation mechanism to promote the awareness and satisfactory degree of YRH services.

Literature describing recovery of left ventricular (LV) function post sacubitril/valsartan treatment and the optimal management of heart failure (HF) patients receiving sacubitril/valsartan remain sparse.We recruited 437 consecutive chronic HF patients with baseline left ventricular ejection fraction (LVEF) less than 40%, who were treated with sacubitril/valsartan. All patients underwent routine echocardiographic measurement.During treatment period, recovery of LVEF to 50% or greater was observed in 77 (17.6%) patients. After multivariate analysis, recovery of LV dysfunction was associated with non-ischemic etiology of HF, smaller baseline LV end-diastolic diameter (LVEDD), and higher initial dosage of sacubitril/valsartan. Compared to those without recovery of LV dysfunction, death from cardiovascular causes or first unplanned hospitalization for HF (CVD/HFH) were significantly lower in patients with LVEF recovery [11.7% vs. 24.4%, hazard ratio (HR) 0.42, p = 0.014]. Among patients with recovery of LVEF, 51 patients continued to receive the same dosage of sacubitril/valsartan had higher LVEF and were less likely to have deterioration of LVEF than the other 26 patients who received either tapering dose of sacubitril/valsartan or switching from sacubitril/valsartan to renin-angiotensin-system blockers (LVEF 56.4 ± 5.3% vs. 45.0 ± 12.8%, p 0.001; ΔLVEF 1.2 ± 5.1% vs. -9.3 ± 12.0%, p 0.001). CVD/HFH occurred more frequently in the taper group than the maintenance group (23.1% vs. 5.9%, HR 0.22, p = 0.035).Non-ischemic etiology of HF, smaller baseline LVEDD, and higher initial dosage of sacubitril/valsartan could predict better recovery of LV function. Among patients with functional recovery, tapering sacubitril/valsartan dose was associated with deterioration of recovered heart function and had less favorable prognosis during follow-up.