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Time-lapse elastic full waveform inversion is used to monitor the spatio-temporal evolution of the CO2 plume during and after supercritical CO2 injection based on a series of time-lapse (repeated) cross-well seismic monitoring datasets obtained at the Nagaoka Carbon Capture and Storage (CCS) site in Japan. The full waveform inversion method successfully estimates the time-lapse velocity decrease of up to 30% within a thin 12 m layer, which is consistent with the magnitude and thickness of the well-log measurements. After the second monitoring survey, the velocity decrease becomes stable and gradually extends down dip along pre-existing geological structures. The full waveform inversion results starkly contrast with the previous estimates based on traveltime tomography. The previous traveltime tomography applications only used the traveltime-delays and resulted in low resolution with few percentage change which was not adequate to correctly resolve CO2 injection changes. The datasets pose significant challenges due to background noise, tube waves, apparent non-isotropic source radiation patterns, apparent reservoir velocity anisotropy and missing key acquisition parameters such as the number of stacks per shot point. To overcome these obstacles, we meticulously perform careful data preprocessing integrating both the body waves and tube waves. We develop waveform-based source mechanism estimation to represent non-isotropic source excitation, and then conduct forward modeling studies to constrain the anisotropy model.

With changing climate, it is necessary to investigate how different plants are af- fected by drought, which is the starting point for this project. The proposed project aims to apply Machine Learning tools to learn predictive patterns of Scots pine seedlings in response to drought conditions by measuring the canopy area and growing rate of the seedlings presented in the time-lapse images. There are 5 different families of Scots Pine researched in this project, therefore 5 different sets of time-lapse images will be used as the data set. The research group has previously created a method for finding the canopy area and computing the growth rate for the different families. Furthermore, the seedlings rotate in an individual pattern each day, which could prove to affect their tolerance to drought according to the research group and is currently not being measured. Therefore, we propose a method using an object detection model, such as Mask R-CNN, to detect and find each seedling’s respective region of interest. With the obtained region of interest, the goal will be to apply an object-tracking algorithm, such as a Dense Optical Flow Algorithm. Using different methods, such as the Shi-Tomasi or Lucas Kanade method, we aim to find feature points and track motion between images to find the direction and velocity of the rotation for each seedling. The tracking algorithms will then be evaluated based on their performance in estimating the rotation features against an annotated sub-set of the time-lapse data set.

Arbuscular mycorrhizal (AM) symbiosis, the nutritional partnership between AM fungi and most plant species, is globally ubiquitous and of great ecological and agricultural importance. Studying the processes of AM symbiosis is confounded by its highly spatiotemporally dynamic nature. While microscopy methods exist to probe the spatial side of this plant–fungal interaction, the temporal side remains more challenging, as reliable deep-tissue time-lapse imaging requires both symbiotic partners to remain undisturbed over prolonged time periods. Here, we introduce the AMSlide: a noninvasive, high-resolution, live-imaging system optimised for AM symbiosis research. We demonstrate the AMSlide's applications in confocal microscopy of mycorrhizal roots, from whole colonisation zones to subcellular structures, over timeframes from minutes to weeks. The AMSlide's versatility for different microscope set-ups, imaging techniques, and plant and fungal species is also outlined. It is hoped that the AMSlide will be applied in future research to fill in the temporal blanks in our understanding of AM symbiosis, as well as broader root and rhizosphere processes.

With changing climate, it is necessary to investigate how different plants are af- fected by drought, which is the starting point for this project. The proposed project aims to apply Machine Learning tools to learn predictive patterns of Scots pine seedlings in response to drought conditions by measuring the canopy area and growing rate of the seedlings presented in the time-lapse images. There are 5 different families of Scots Pine researched in this project, therefore 5 different sets of time-lapse images will be used as the data set. The research group has previously created a method for finding the canopy area and computing the growth rate for the different families. Furthermore, the seedlings rotate in an individual pattern each day, which could prove to affect their tolerance to drought according to the research group and is currently not being measured. Therefore, we propose a method using an object detection model, such as Mask R-CNN, to detect and find each seedling’s respective region of interest. With the obtained region of interest, the goal will be to apply an object-tracking algorithm, such as a Dense Optical Flow Algorithm. Using different methods, such as the Shi-Tomasi or Lucas Kanade method, we aim to find feature points and track motion between images to find the direction and velocity of the rotation for each seedling. The tracking algorithms will then be evaluated based on their performance in estimating the rotation features against an annotated sub-set of the time-lapse data set.

We conducted a time-lapse seismic experiment utilizing automated active seismic source and sensor arrays to monitor a reactivated fault within the Opalinus clay formation at the Mont Terri Rock Laboratory (Switzerland), an analog caprock for geologic carbon storage. A series of six brine injections were conducted into the so-called Main Fault to reactivate it. Seismic instrumentation in five monitoring boreholes on either side of the fault was used to continuously probe changes in P-wave travel-times associated with fault displacement and leakage. We performed time-lapse travel-time tomography on five hundred sequential data sets; this revealed a zone of decreased P-wave velocity, up to 16 m/s, during each injection cycle, followed by a velocity increase during shut-in. These results demonstrate varying elastic property perturbations, both spatially and temporally, along the fault plane during reactivation. We then interpreted these velocity changes in terms of fault dilation induced by pressurized fluids along the fault.

STUDY QUESTION: Is embryo culture in a closed time-lapse system associated with any differences in perinatal and maternal outcomes in comparison to conventional culture and spontaneous conception? SUMMARY ANSWER: There were no significant differences between time-lapse and conventional embryo culture in preterm birth (PTB, <37 weeks), low birth weight (LBW, >2500 g) and hypertensive disorders of pregnancy for singleton deliveries, the primary outcomes of this study. WHAT IS KNOWN ALREADY: Evidence from prospective trials evaluating the safety of time-lapse incubation for clinical use show similar embryo development rates, implantation rates, and ongoing pregnancy and live birth rates when compared to conventional incubation. Few studies have investigated if uninterrupted culture can alter risks of adverse perinatal outcomes presently associated with IVF when compared to conventional culture and spontaneous conceptions. STUDY DESIGN, SIZE, DURATION: This study is a Swedish population-based retrospective registry study, including 7379 singleton deliveries after fresh embryo transfer between 2013 and 2018 from selected IVF clinics. Perinatal outcomes of singletons born from time-lapse-cultured embryos were compared to singletons from embryos cultured in conventional incubators and 71 300 singletons from spontaneous conceptions. Main perinatal outcomes included PTB and LBW. Main maternal outcomes included hypertensive disorders of pregnancy (pregnancy hypertension and preeclampsia). PARTICIPANTS/MATERIALS, SETTING, METHODS: From nine IVF clinics, 2683 singletons born after fresh embryo transfer in a time-lapse system were compared to 4696 singletons born after culture in a conventional incubator and 71 300 singletons born after spontaneous conception matched for year of birth, parity, and maternal age. Patient and treatment characteristics from IVF deliveries were cross-linked with the Swedish Medical Birth Register, Register of Birth Defects, National Patient Reg

STUDY QUESTION: Is spontaneous collapse (SC) by human blastocysts a prognostic factor in IVF treatment? SUMMARY ANSWER: SC in human blastocyst is associated with reduced euploid embryo and pregnancy rates. WHAT IS KNOWN ALREADY: SC of the human blastocyst is a phenomenon that was revealed relatively recently following the clinical application of time-lapse monitoring in IVF laboratories. The ploidy and clinical prognosis of affected blastocysts are still poorly understood, with inconsistent reports. Systematic reviews and meta-analyses on this topic are currently absent in the literature but its potential as a marker of embryo viability holds great clinical value. In this study, we aimed to comprehensively evaluate the potential of SC as a prognostic factor in regard to ploidy status, and pregnancy, live birth and miscarriage rates. STUDY DESIGN, SIZE, DURATION: A systematic review and meta-analysis were performed according to PRISMA guidelines, with a protocol registered with PROSPERO (CRD42022373749). A search of MEDLINE, EMBASE, and the Cochrane Library for relevant studies was carried out on 10 October 2022, using key words relevant to 'blastocyst collapse' and 'time-lapse imaging'. PARTICIPANTS/MATERIALS, SETTING, METHODS: Two independent reviewers systematically screened and evaluated each study in terms of participants, exposure, comparator, and outcomes (PECO). The Quality In Prognosis Studies tool was used for quality assessment. Data were extracted according to Cochrane methods. Pregnancy, live birth, ploidy, or miscarriage data were summarized by risk ratios (RRs) or odds ratios and their 95% CIs. All meta-analyses were performed with random-effects models. MAIN RESULTS AND THE ROLE OF CHANCE: Following removal of duplicates, a total of 196 records were identified by the initial search. After screening according to PECO, 19 articles were included for further eligibility assessment. For meta-analysis, seven retrospective cohort studies were eventually incl

Gas leakage may occur in pipelines or subsurface storage sites. The appearance of a new gas phase in the geological subsurface can impede groundwater flow and increase total media compressibility, with both effects increasing with larger gas phase volumes. In near-surface aquifers, these changes can be assessed by determining changes in hydraulic conductivity and specific storage using conventional pumping tests. These allow an observer to detect and delineate the occurrence of a new gas phase. In this study, this idea was tested using methane gas and hydrogen gas injection experiments, which were conducted at the TestUM field site in Germany. A series of multi-level cross-well and depth-oriented pumping tests were conducted before and after injection of the hydrogen and methane gas phases. The results show a significant decrease of hydraulic conductivity and a significant increase of specific storage estimated from the drawdown data due to the presence of the gas phases. Hydraulic conductivity and specific storage were found to vary by up to 86% and 65,900% respectively after gas phase injection, while repeated baseline tests yielded a variation of less than 28% and 340% prior to gas phase injection. These changes are correlated, and their occurrence and magnitude can be used to roughly locate the gas phases. As only standard hydrogeological field equipment and evaluation techniques are required, this approach represents a new monitoring option to detect an intruding gas phase in near-surface aquifers.

Monitoring time-lapse changes inside the subsurface is of great significance to many geotechnical applications, such as storage of gasses in underground geological formations. Minute differences in the seismic wavefield between an initial baseline and a subsequent monitor survey have to be detected in order to observe fluid flow inside subsurface reservoirs. This problem becomes even more challenging when the reservoir is situated underneath a series of complex, highly reflective layers. Such an overburden will generate strong multiple reflections that will interfere with the reflections of the target zone. Ideally, a methodology is designed in order to remove these internal multiples to allow a clear view of the reservoir response for time-lapse analysis. The Marchenko method can redatumthe seismic wavefield to arbitrary depth levels or points in the subsurface, while accounting for all orders of internal multiple reflections. This method, therefore, has great potential to solve some of the time-lapse issues, as it is able to closely examine specific zones of interest in the subsurface without distortions from surrounding layers. Time-lapse studies are often hampered by irregular or imperfect sampling, whereas the Marchenko method relies on densely sampled, co-located sources and receivers. It is, therefore, important that the Marchenko method is able to handle more complex acquisition geometries. This can either be achieved by interpolating the reflection data as a pre-processing step or by correcting for errors inside the Marchenko scheme. Here, point-spread functions are introduced that describe the imperfections in the reflection data. These imperfections distort the focusing and Green’s functions retrieved from the Marchenko method. Next, each iteration of theMarchenko scheme is extended to deblur the imperfect focusing and Green’s functions by multidimensional deconvolution with these point-spread functions. Additionally, a slight modification is required to, Applied Geophysics and Petrophysics

This work aimed to study the response of Uruguayan consumers to cheeses with different nutritional and commercial characteristics and determine changes over the last decade. Conjoint analysis was used to establish the value that consumers gave to different health benefits (low-fat, salt-reduced, fibre-enriched, probiotic) and to different manufacturing processes (traditional and industrial) for two different types of cheese (spreadable and semi-soft). Results indicated that consumers’ interest varied depending on the proposed health benefit. In general consumers’ interest in cheese with specific nutritional benefits was lower than for the regular product. Consumers were interested only in the low-fat cheeses with no change in 2010 and 2018. Manufacture type became significant between years, nowadays consumers are more interested in traditional manufacture. Consumer segmentation in 2018 showed that some groups were willing to consume cheeses with health benefits, among the different options, nowadays there are new opportunities for functionality in cheeses.

We report on the extraction of deep ocean travel time variations from time-lapse cross-correlations between a hydrophone station and a three-component broadband seismometer. The signals we cross-correlate in this study result from repeated activity by the Monowai seamount, one of the most active submarine volcanoes of the Tonga-Kermadec ridge. In particular, we introduce a specific workflow to exploit repetitive hydroacoustic underwater source activity, which we detail to such an extent that it serves as an example (or “cookbook”). For this reason, we have made the source code publicly available. The workflow proposed in this study (a) overcomes differences in instrument sensitivity and sample rates, (b) involves the selection of eligible cross-correlations based on a source activity criterium as well as slowness analysis, and (c) extracts the travel time variations in distinct frequency bands. In our case, the two frequency bands are 3–6 and 6–12 Hz. We find that the estimated travel time variations in both frequency bands consist of a complex periodic pattern superimposed on a robust linear trend. This linear trend is decreasing, which we attribute to increasing water temperatures along the propagation path of the hydroacoustic signals., Applied Geophysics and Petrophysics

Patients with chronic kidney disease – mineral and bone disorder, a disease that results in a high risk for bone fractures, can be classified based on the rate of total bone turnover. Currently, bone turnover rates are determined from iliac crest bone biopsies followed by quantitative histomorphometry. However, this method is painful, invasive, and performed in an area not typically prone to fractures. In this ongoing prospective study, we explore the use of time-lapse high resolution peripheral quantitative computed tomography (HR-pQCT) imaging as a non-invasive method to determine bone turnover in patients. Fifteen participants are expected to participate in this study. To date, six participants were recruited to undergo HR-pQCT scans at four different timepoints with two-month intervals. Three scans will be performed at the first timepoint and one scan will be performed at the three following timepoints. The three repeat baseline scans and each of the follow-up scans underwent time-lapse analysis to determine total bone turnover. For this thesis project, the objective was to establish methodology for this study, including determine the time-lapse threshold needed to detect <0.5% reproducibility differences, determine the more robust image processing pipeline between two processing techniques, determine the least significant change (LSC) from baseline repeat scans, and determine if there are detectable time-lapse changes in total bone turnover at two-months. The preliminary results determined that using a Laplace-Hamming image processing pipeline performed better than the standard analysis protocol, determined that a threshold of 525 mgHA/ccm led to <0.5% reproducibility differences, demonstrated a LSC of 0.088 and 0.040 for the radius and tibia respectively, and showed that after two months, time-lapse HR-pQCT could measure in vivo total bone turnover in patients but not greater than the LSC values. Since determining the optimal time to scan was an objectiv

Electrical resistivity has been used as a noninvasive geophysical technique for locating clandestine graves and monitoring human decay within the subsurface. Detailed studies assessing resistivity anomalies due to soil disturbances and decay products associated with graves have relied on the use of proxies, such as pigs, with limited studies using real human cadavers and simulating a mass grave setting and none assessing the anomalies in 3D. In this study, we used time-lapse 2D and quasi-3D electrical resistivity distribution measured over an experimental mass grave and individual graves containing human cadavers to assess resistivity anomalies resulting from graves and the presence of decaying human remains in them. This study is part of a novel multidisciplinary mass grave experimental study with six graves consisting of a mass grave with six human cadavers, a controlled mass grave with none, three individual graves with one human cadaver each, and a control individual grave with none. Nine parallel resistivity transects which allow us to image these graves in their 3D context were acquired prior to excavation and 2 days, 1, 2, and 6 months after burial using a dipole-dipole electrode array, a unit electrode spacing of 0.5 m, and an interprofile spacing of 1m. The value of different electrode arrays and spacings in identifying the contrast between the graves was also assessed using forward models and field data. Soil sensors were installed at different locations in the graves to monitor soil electrical conductivity, moisture content, and temperature. The results of this study show an increase in electrical resistivity 2 days after burial in all graves with human remains and the control graves, which we attribute to increased soil aeration where disturbed pores are filled with air. The resistivity decreases thereafter in graves with human remains which we attribute to the formation of conductive leachates. This study validates the potential of electrical resistivit

Electrical resistivity has been used as a noninvasive geophysical technique for locating clandestine graves and monitoring human decay within the subsurface. Detailed studies assessing resistivity anomalies due to soil disturbances and decay products associated with graves have relied on the use of proxies, such as pigs, with limited studies using real human cadavers and simulating a mass grave setting and none assessing the anomalies in 3D. In this study, we used time-lapse 2D and quasi-3D electrical resistivity distribution measured over an experimental mass grave and individual graves containing human cadavers to assess resistivity anomalies resulting from graves and the presence of decaying human remains in them. This study is part of a novel multidisciplinary mass grave experimental study with six graves consisting of a mass grave with six human cadavers, a controlled mass grave with none, three individual graves with one human cadaver each, and a control individual grave with none. Nine parallel resistivity transects which allow us to image these graves in their 3D context were acquired prior to excavation and 2 days, 1, 2, and 6 months after burial using a dipole-dipole electrode array, a unit electrode spacing of 0.5 m, and an interprofile spacing of 1m. The value of different electrode arrays and spacings in identifying the contrast between the graves was also assessed using forward models and field data. Soil sensors were installed at different locations in the graves to monitor soil electrical conductivity, moisture content, and temperature. The results of this study show an increase in electrical resistivity 2 days after burial in all graves with human remains and the control graves, which we attribute to increased soil aeration where disturbed pores are filled with air. The resistivity decreases thereafter in graves with human remains which we attribute to the formation of conductive leachates. This study validates the potential of electrical resistivit

Patients with chronic kidney disease – mineral and bone disorder, a disease that results in a high risk for bone fractures, can be classified based on the rate of total bone turnover. Currently, bone turnover rates are determined from iliac crest bone biopsies followed by quantitative histomorphometry. However, this method is painful, invasive, and performed in an area not typically prone to fractures. In this ongoing prospective study, we explore the use of time-lapse high resolution peripheral quantitative computed tomography (HR-pQCT) imaging as a non-invasive method to determine bone turnover in patients. Fifteen participants are expected to participate in this study. To date, six participants were recruited to undergo HR-pQCT scans at four different timepoints with two-month intervals. Three scans will be performed at the first timepoint and one scan will be performed at the three following timepoints. The three repeat baseline scans and each of the follow-up scans underwent time-lapse analysis to determine total bone turnover. For this thesis project, the objective was to establish methodology for this study, including determine the time-lapse threshold needed to detect <0.5% reproducibility differences, determine the more robust image processing pipeline between two processing techniques, determine the least significant change (LSC) from baseline repeat scans, and determine if there are detectable time-lapse changes in total bone turnover at two-months. The preliminary results determined that using a Laplace-Hamming image processing pipeline performed better than the standard analysis protocol, determined that a threshold of 525 mgHA/ccm led to <0.5% reproducibility differences, demonstrated a LSC of 0.088 and 0.040 for the radius and tibia respectively, and showed that after two months, time-lapse HR-pQCT could measure in vivo total bone turnover in patients but not greater than the LSC values. Since determining the optimal time to scan was an objectiv

Electrical resistivity has been used as a noninvasive geophysical technique for locating clandestine graves and monitoring human decay within the subsurface. Detailed studies assessing resistivity anomalies due to soil disturbances and decay products associated with graves have relied on the use of proxies, such as pigs, with limited studies using real human cadavers and simulating a mass grave setting and none assessing the anomalies in 3D. In this study, we used time-lapse 2D and quasi-3D electrical resistivity distribution measured over an experimental mass grave and individual graves containing human cadavers to assess resistivity anomalies resulting from graves and the presence of decaying human remains in them. This study is part of a novel multidisciplinary mass grave experimental study with six graves consisting of a mass grave with six human cadavers, a controlled mass grave with none, three individual graves with one human cadaver each, and a control individual grave with none. Nine parallel resistivity transects which allow us to image these graves in their 3D context were acquired prior to excavation and 2 days, 1, 2, and 6 months after burial using a dipole-dipole electrode array, a unit electrode spacing of 0.5 m, and an interprofile spacing of 1m. The value of different electrode arrays and spacings in identifying the contrast between the graves was also assessed using forward models and field data. Soil sensors were installed at different locations in the graves to monitor soil electrical conductivity, moisture content, and temperature. The results of this study show an increase in electrical resistivity 2 days after burial in all graves with human remains and the control graves, which we attribute to increased soil aeration where disturbed pores are filled with air. The resistivity decreases thereafter in graves with human remains which we attribute to the formation of conductive leachates. This study validates the potential of electrical resistivit

Electrical resistivity has been used as a noninvasive geophysical technique for locating clandestine graves and monitoring human decay within the subsurface. Detailed studies assessing resistivity anomalies due to soil disturbances and decay products associated with graves have relied on the use of proxies, such as pigs, with limited studies using real human cadavers and simulating a mass grave setting and none assessing the anomalies in 3D. In this study, we used time-lapse 2D and quasi-3D electrical resistivity distribution measured over an experimental mass grave and individual graves containing human cadavers to assess resistivity anomalies resulting from graves and the presence of decaying human remains in them. This study is part of a novel multidisciplinary mass grave experimental study with six graves consisting of a mass grave with six human cadavers, a controlled mass grave with none, three individual graves with one human cadaver each, and a control individual grave with none. Nine parallel resistivity transects which allow us to image these graves in their 3D context were acquired prior to excavation and 2 days, 1, 2, and 6 months after burial using a dipole-dipole electrode array, a unit electrode spacing of 0.5 m, and an interprofile spacing of 1m. The value of different electrode arrays and spacings in identifying the contrast between the graves was also assessed using forward models and field data. Soil sensors were installed at different locations in the graves to monitor soil electrical conductivity, moisture content, and temperature. The results of this study show an increase in electrical resistivity 2 days after burial in all graves with human remains and the control graves, which we attribute to increased soil aeration where disturbed pores are filled with air. The resistivity decreases thereafter in graves with human remains which we attribute to the formation of conductive leachates. This study validates the potential of electrical resistivit

We report on the extraction of deep ocean travel time variations from time-lapse cross-correlations between a hydrophone station and a three-component broadband seismometer. The signals we cross-correlate in this study result from repeated activity by the Monowai seamount, one of the most active submarine volcanoes of the Tonga-Kermadec ridge. In particular, we introduce a specific workflow to exploit repetitive hydroacoustic underwater source activity, which we detail to such an extent that it serves as an example (or “cookbook”). For this reason, we have made the source code publicly available. The workflow proposed in this study (a) overcomes differences in instrument sensitivity and sample rates, (b) involves the selection of eligible cross-correlations based on a source activity criterium as well as slowness analysis, and (c) extracts the travel time variations in distinct frequency bands. In our case, the two frequency bands are 3–6 and 6–12 Hz. We find that the estimated travel time variations in both frequency bands consist of a complex periodic pattern superimposed on a robust linear trend. This linear trend is decreasing, which we attribute to increasing water temperatures along the propagation path of the hydroacoustic signals., Applied Geophysics and Petrophysics

Electrical resistivity has been used as a noninvasive geophysical technique for locating clandestine graves and monitoring human decay within the subsurface. Detailed studies assessing resistivity anomalies due to soil disturbances and decay products associated with graves have relied on the use of proxies, such as pigs, with limited studies using real human cadavers and simulating a mass grave setting and none assessing the anomalies in 3D. In this study, we used time-lapse 2D and quasi-3D electrical resistivity distribution measured over an experimental mass grave and individual graves containing human cadavers to assess resistivity anomalies resulting from graves and the presence of decaying human remains in them. This study is part of a novel multidisciplinary mass grave experimental study with six graves consisting of a mass grave with six human cadavers, a controlled mass grave with none, three individual graves with one human cadaver each, and a control individual grave with none. Nine parallel resistivity transects which allow us to image these graves in their 3D context were acquired prior to excavation and 2 days, 1, 2, and 6 months after burial using a dipole-dipole electrode array, a unit electrode spacing of 0.5 m, and an interprofile spacing of 1m. The value of different electrode arrays and spacings in identifying the contrast between the graves was also assessed using forward models and field data. Soil sensors were installed at different locations in the graves to monitor soil electrical conductivity, moisture content, and temperature. The results of this study show an increase in electrical resistivity 2 days after burial in all graves with human remains and the control graves, which we attribute to increased soil aeration where disturbed pores are filled with air. The resistivity decreases thereafter in graves with human remains which we attribute to the formation of conductive leachates. This study validates the potential of electrical resistivit

The important role of mitochondria in maintaining normal brain cell function has been demonstrated in several neurodegenerative diseases where mitochondrial dysfunction is a prominent feature. Accumulating evidence indicates that opioids may induce neuronal cell death and inhibit neurogenesis, two factors that are dependent on normal mitochondrial function. The aim of the present study was to examine the effects of morphine, methadone, and fentanyl on mitochondrial morphology. Cells from the neuroblastoma/glioma hybrid cell-line NG108-15 were seeded on 96-well cell culture plates and treated with MitoTracker™ for 30 min prior to opioid treatment. Morphine, methadone, and fentanyl were added at various concentrations and images of mitochondria were acquired every 30 min for four hours using a high-content imaging device. The morphological parameters total mitochondrial area, mitochondrial network, number of mitochondrial objects, and the mean area of mitochondrial objects were analyzed using automated image analysis. Methadone and fentanyl, but not morphine, decreased the mitochondrial network, the number of mitochondrial objects, and increased the mean area of mitochondrial objects. Both methadone and fentanyl altered mitochondrial morphology with no effects seen from morphine treatment. These data suggest that methadone and fentanyl disrupt mitochondrial morphology, which may contribute to neuronal cell death., Title in dissertation list of papers: The effects of morphine, methadone, and fentanyl on mitochondrial morphology: a live cell imaging study

4D seismic studies aim to observe time-lapse changes in the subsurface between a baseline and a monitor study. These changes are generally small, and the seismic response from a deep reservoir can be concealed by reflections from shallow structures. Here, we introduce a novel way of isolating the reservoir response by means of the Marchenko method. First, this two-step approach removes the overburden interactions, and the second step eliminates the underburden reflections. Finally, the difference in traveltime is found by correlation of the (isolated) responses of the baseline and monitor study. An additional benefit of isolating the reservoir response is that internal multiples are more distinctly observed. These multiples have propagated through the reservoir more than once, therefore, their response experiences a larger traveltime perturbation, and provides further confirmation on the traveltime difference between the 4D studies., Accepted Author Manuscript, Applied Geophysics and Petrophysics, ImPhys/Medical Imaging

Over time, insects have developed complex strategies to defend themselves against presenting threats. However, in the evolutionary arms race of survival, pathogens have adapted to quickly overcome the immune response mounted by the host. In this thesis, we assess how quickly entomopathogenic nematodes (EPNs) can overcome the host, Drosophila melanogaster. We then look at the clotting reaction at a hypothetical point of entry for the nematode and bring resolution to the order of protein interaction focusing on three proteins important in the anti-nematode defense. Finally, we look closer into detail at how crystal cells secrete one of those proteins, prophenoloxidase (PPOII) using a mode of programmed cell death. (Paper I) In the course of EPN infection, little was known about how quickly the worms can overcome the host immune system. Here we found that after penetrating the host, EPNs cause septicemia within 4 to 6 hours. (Paper II) Three proteins, Glutactin (Glt), Transglutaminase (Tg), and PPOII have been found to be important in the anti-nematode response. Here we created GFP-tagged fly constructs to follow their role in clot formation. In early clot formation, Tg was immediately secreted from hemocytes though it was localized around the cell membrane, Glt then entered clot fibers followed by PPOII which acted in late clot formation. (Paper III) Here we looked closer into Tg and PPOII secretion variability. PPOII from immature, but not mature crystal cells colocalized with a membrane marker. Tg, when driven with a pan tissue driver, was found located in clotting fibers, in contrast with paper II. (Paper IV) In an in vivo immune scenario, crystal cells were recruited to the wound site and burst rapidly in a caspase-dependent manner. We demonstrate that the mode of programmed cell death, pyroptosis, exists in Drosophila by way of convergent evolution. This thesis brings to light the variation found within the infection process for EPNs as well as the clotting resp

Sea level rise concerns millions of people in coastal areas across the globe. One of the largest uncertainties to project future sea level rise is the frontal ablation (accounting for calving and submarine melt) at marine ice margins, around the Greenland and Antarctic Ice Sheet. High rates of frontal ablation have been observed to imply, through loss of the buttressing effect but not limited to it, increased mass loss from marine terminating glaciers and hence, associated sea level rise. This study focuses on calving processes at a freshwater lake in northern Sweden, which represents a simpler environment to study calving processes than the marine one, because impacts of tides, salinity, and circulation (all known to be relevant at marine ice-ocean boundaries) can be neglected. A multi-method approach to quantify and characterize calving events is presented here, exploring and analysing the underwater acoustic soundscape at a calving glacier front, in connection with optical, image-based methods such as time- lapse photography, and photogrammetry based on footage acquired by an uncrewed aerial vehicle (UAV). An acoustic detector is developed, tested and applied to data set acquired during 2020, and results indicate that the acoustic detector can be an important complement in the range of tools used to observe, and quantify, calving. Applied in remote locations, where continuous monitoring is difficult and where optical methods are of limited use, collecting acoustic data and monitoring calving by means of its acoustic signature could render insights previously not available (because of lacking data and methodology).

Over time, insects have developed complex strategies to defend themselves against presenting threats. However, in the evolutionary arms race of survival, pathogens have adapted to quickly overcome the immune response mounted by the host. In this thesis, we assess how quickly entomopathogenic nematodes (EPNs) can overcome the host, Drosophila melanogaster. We then look at the clotting reaction at a hypothetical point of entry for the nematode and bring resolution to the order of protein interaction focusing on three proteins important in the anti-nematode defense. Finally, we look closer into detail at how crystal cells secrete one of those proteins, prophenoloxidase (PPOII) using a mode of programmed cell death. (Paper I) In the course of EPN infection, little was known about how quickly the worms can overcome the host immune system. Here we found that after penetrating the host, EPNs cause septicemia within 4 to 6 hours. (Paper II) Three proteins, Glutactin (Glt), Transglutaminase (Tg), and PPOII have been found to be important in the anti-nematode response. Here we created GFP-tagged fly constructs to follow their role in clot formation. In early clot formation, Tg was immediately secreted from hemocytes though it was localized around the cell membrane, Glt then entered clot fibers followed by PPOII which acted in late clot formation. (Paper III) Here we looked closer into Tg and PPOII secretion variability. PPOII from immature, but not mature crystal cells colocalized with a membrane marker. Tg, when driven with a pan tissue driver, was found located in clotting fibers, in contrast with paper II. (Paper IV) In an in vivo immune scenario, crystal cells were recruited to the wound site and burst rapidly in a caspase-dependent manner. We demonstrate that the mode of programmed cell death, pyroptosis, exists in Drosophila by way of convergent evolution. This thesis brings to light the variation found within the infection process for EPNs as well as the clotting resp

Fluid flow through fractured rock systems is governed in large part by the distribution, interconnectivity, and size of fracture apertures. In-situ stress is one of the primary factors controlling fracture aperture, and one that is altered significantly during high-pressure fluid injections or extractions. Interactions between stress, pore pressure, aperture, and fluid flow can result in complex and evolving poroelastic behavior with significant implications regarding the predictability and risk involved with developing and managing deep subsurface reservoirs (geothermal, fossil energy, and geologic carbon sequestration). In saturated rocks, bulk electrical conductivity is sensitive to both primary and secondary porosity (i.e., matrix porosity and fractures), and therefore to fracture aperture size and distribution. We demonstrate the use of time-lapse 3D electrical resistivity tomography for remotely monitoring stress induced changes in aperture distribution during high pressure injections into a dense fractured rock system at a scale of tens of meters. Results reveal a complex and continuously evolving stress field involving aperture dilations in the natural fracture system and aperture contractions in adjacent zones of shadow stress. Results provide information about the spatiotemporal changes in the system behavior and point to the potential of electrical imaging for autonomously and remotely monitoring evolving stress conditions by proxy through changes in bulk electrical conductivity.

Over time, insects have developed complex strategies to defend themselves against presenting threats. However, in the evolutionary arms race of survival, pathogens have adapted to quickly overcome the immune response mounted by the host. In this thesis, we assess how quickly entomopathogenic nematodes (EPNs) can overcome the host, Drosophila melanogaster. We then look at the clotting reaction at a hypothetical point of entry for the nematode and bring resolution to the order of protein interaction focusing on three proteins important in the anti-nematode defense. Finally, we look closer into detail at how crystal cells secrete one of those proteins, prophenoloxidase (PPOII) using a mode of programmed cell death. (Paper I) In the course of EPN infection, little was known about how quickly the worms can overcome the host immune system. Here we found that after penetrating the host, EPNs cause septicemia within 4 to 6 hours. (Paper II) Three proteins, Glutactin (Glt), Transglutaminase (Tg), and PPOII have been found to be important in the anti-nematode response. Here we created GFP-tagged fly constructs to follow their role in clot formation. In early clot formation, Tg was immediately secreted from hemocytes though it was localized around the cell membrane, Glt then entered clot fibers followed by PPOII which acted in late clot formation. (Paper III) Here we looked closer into Tg and PPOII secretion variability. PPOII from immature, but not mature crystal cells colocalized with a membrane marker. Tg, when driven with a pan tissue driver, was found located in clotting fibers, in contrast with paper II. (Paper IV) In an in vivo immune scenario, crystal cells were recruited to the wound site and burst rapidly in a caspase-dependent manner. We demonstrate that the mode of programmed cell death, pyroptosis, exists in Drosophila by way of convergent evolution. This thesis brings to light the variation found within the infection process for EPNs as well as the clotting resp

Sea level rise concerns millions of people in coastal areas across the globe. One of the largest uncertainties to project future sea level rise is the frontal ablation (accounting for calving and submarine melt) at marine ice margins, around the Greenland and Antarctic Ice Sheet. High rates of frontal ablation have been observed to imply, through loss of the buttressing effect but not limited to it, increased mass loss from marine terminating glaciers and hence, associated sea level rise. This study focuses on calving processes at a freshwater lake in northern Sweden, which represents a simpler environment to study calving processes than the marine one, because impacts of tides, salinity, and circulation (all known to be relevant at marine ice-ocean boundaries) can be neglected. A multi-method approach to quantify and characterize calving events is presented here, exploring and analysing the underwater acoustic soundscape at a calving glacier front, in connection with optical, image-based methods such as time- lapse photography, and photogrammetry based on footage acquired by an uncrewed aerial vehicle (UAV). An acoustic detector is developed, tested and applied to data set acquired during 2020, and results indicate that the acoustic detector can be an important complement in the range of tools used to observe, and quantify, calving. Applied in remote locations, where continuous monitoring is difficult and where optical methods are of limited use, collecting acoustic data and monitoring calving by means of its acoustic signature could render insights previously not available (because of lacking data and methodology).

4D seismic studies aim to observe time-lapse changes in the subsurface between a baseline and a monitor study. These changes are generally small, and the seismic response from a deep reservoir can be concealed by reflections from shallow structures. Here, we introduce a novel way of isolating the reservoir response by means of the Marchenko method. First, this two-step approach removes the overburden interactions, and the second step eliminates the underburden reflections. Finally, the difference in traveltime is found by correlation of the (isolated) responses of the baseline and monitor study. An additional benefit of isolating the reservoir response is that internal multiples are more distinctly observed. These multiples have propagated through the reservoir more than once, therefore, their response experiences a larger traveltime perturbation, and provides further confirmation on the traveltime difference between the 4D studies., Accepted Author Manuscript, Applied Geophysics and Petrophysics, ImPhys/Medical Imaging

Over time, insects have developed complex strategies to defend themselves against presenting threats. However, in the evolutionary arms race of survival, pathogens have adapted to quickly overcome the immune response mounted by the host. In this thesis, we assess how quickly entomopathogenic nematodes (EPNs) can overcome the host, Drosophila melanogaster. We then look at the clotting reaction at a hypothetical point of entry for the nematode and bring resolution to the order of protein interaction focusing on three proteins important in the anti-nematode defense. Finally, we look closer into detail at how crystal cells secrete one of those proteins, prophenoloxidase (PPOII) using a mode of programmed cell death. (Paper I) In the course of EPN infection, little was known about how quickly the worms can overcome the host immune system. Here we found that after penetrating the host, EPNs cause septicemia within 4 to 6 hours. (Paper II) Three proteins, Glutactin (Glt), Transglutaminase (Tg), and PPOII have been found to be important in the anti-nematode response. Here we created GFP-tagged fly constructs to follow their role in clot formation. In early clot formation, Tg was immediately secreted from hemocytes though it was localized around the cell membrane, Glt then entered clot fibers followed by PPOII which acted in late clot formation. (Paper III) Here we looked closer into Tg and PPOII secretion variability. PPOII from immature, but not mature crystal cells colocalized with a membrane marker. Tg, when driven with a pan tissue driver, was found located in clotting fibers, in contrast with paper II. (Paper IV) In an in vivo immune scenario, crystal cells were recruited to the wound site and burst rapidly in a caspase-dependent manner. We demonstrate that the mode of programmed cell death, pyroptosis, exists in Drosophila by way of convergent evolution. This thesis brings to light the variation found within the infection process for EPNs as well as the clotting resp

Over time, insects have developed complex strategies to defend themselves against presenting threats. However, in the evolutionary arms race of survival, pathogens have adapted to quickly overcome the immune response mounted by the host. In this thesis, we assess how quickly entomopathogenic nematodes (EPNs) can overcome the host, Drosophila melanogaster. We then look at the clotting reaction at a hypothetical point of entry for the nematode and bring resolution to the order of protein interaction focusing on three proteins important in the anti-nematode defense. Finally, we look closer into detail at how crystal cells secrete one of those proteins, prophenoloxidase (PPOII) using a mode of programmed cell death. (Paper I) In the course of EPN infection, little was known about how quickly the worms can overcome the host immune system. Here we found that after penetrating the host, EPNs cause septicemia within 4 to 6 hours. (Paper II) Three proteins, Glutactin (Glt), Transglutaminase (Tg), and PPOII have been found to be important in the anti-nematode response. Here we created GFP-tagged fly constructs to follow their role in clot formation. In early clot formation, Tg was immediately secreted from hemocytes though it was localized around the cell membrane, Glt then entered clot fibers followed by PPOII which acted in late clot formation. (Paper III) Here we looked closer into Tg and PPOII secretion variability. PPOII from immature, but not mature crystal cells colocalized with a membrane marker. Tg, when driven with a pan tissue driver, was found located in clotting fibers, in contrast with paper II. (Paper IV) In an in vivo immune scenario, crystal cells were recruited to the wound site and burst rapidly in a caspase-dependent manner. We demonstrate that the mode of programmed cell death, pyroptosis, exists in Drosophila by way of convergent evolution. This thesis brings to light the variation found within the infection process for EPNs as well as the clotting resp

Over time, insects have developed complex strategies to defend themselves against presenting threats. However, in the evolutionary arms race of survival, pathogens have adapted to quickly overcome the immune response mounted by the host. In this thesis, we assess how quickly entomopathogenic nematodes (EPNs) can overcome the host, Drosophila melanogaster. We then look at the clotting reaction at a hypothetical point of entry for the nematode and bring resolution to the order of protein interaction focusing on three proteins important in the anti-nematode defense. Finally, we look closer into detail at how crystal cells secrete one of those proteins, prophenoloxidase (PPOII) using a mode of programmed cell death. (Paper I) In the course of EPN infection, little was known about how quickly the worms can overcome the host immune system. Here we found that after penetrating the host, EPNs cause septicemia within 4 to 6 hours. (Paper II) Three proteins, Glutactin (Glt), Transglutaminase (Tg), and PPOII have been found to be important in the anti-nematode response. Here we created GFP-tagged fly constructs to follow their role in clot formation. In early clot formation, Tg was immediately secreted from hemocytes though it was localized around the cell membrane, Glt then entered clot fibers followed by PPOII which acted in late clot formation. (Paper III) Here we looked closer into Tg and PPOII secretion variability. PPOII from immature, but not mature crystal cells colocalized with a membrane marker. Tg, when driven with a pan tissue driver, was found located in clotting fibers, in contrast with paper II. (Paper IV) In an in vivo immune scenario, crystal cells were recruited to the wound site and burst rapidly in a caspase-dependent manner. We demonstrate that the mode of programmed cell death, pyroptosis, exists in Drosophila by way of convergent evolution. This thesis brings to light the variation found within the infection process for EPNs as well as the clotting resp

Focusing on the period since 2007, this thesis aims to deepen understandings of how the contemporary professional UK outdoor arts sector operates, how audiences engage with outdoor arts and how they might contribute to the construction of public space. The research examines UK outdoor arts from two viewpoints: firstly, using [auto]ethnography to study the people who create and produce it; and secondly, adapting Lefebvre’s concept of rhythmanalysis to consider live outdoor arts events, how they are organised, experienced and perceived. The events studied all occurred in the summer of 2018 and the main analysis centres around three case studies which occurred in Greenwich (London), Luton and Doncaster. In order to study ephemeral, outdoor arts events I developed a new, efficient and replicable methodology, which responds to their unique features and can evaluate their ability to intervene in everyday life and produce festivalized space. This uses multiple time-lapse cameras and an embedded, expert researcher to make detailed observations of the triad of core categories identified: space/time, performance and audience. This approach generated both quantitative and qualitative datasets: on the one hand delivering, for the first time, accurate numbers for audience engagements and empirical evidence of audience flows, stay-lengths and proxemics; and on the other hand, documenting atmospheres, connections and identity shifts. The thesis contributes to knowledge about the inspirations, motivations and priorities of those who create and produce outdoor arts today and their relationships to public space. It develops theory around the tendency for outdoor arts events to become more homogenous and increasingly codified in recent years, questioning the validity of the strategies and structures driving this process. It identifies and analyses a range of behaviours and techniques exclusively used by organisers, artists and audiences of outdoor arts and in doing s

Focusing on the period since 2007, this thesis aims to deepen understandings of how the contemporary professional UK outdoor arts sector operates, how audiences engage with outdoor arts and how they might contribute to the construction of public space. The research examines UK outdoor arts from two viewpoints: firstly, using [auto]ethnography to study the people who create and produce it; and secondly, adapting Lefebvre’s concept of rhythmanalysis to consider live outdoor arts events, how they are organised, experienced and perceived. The events studied all occurred in the summer of 2018 and the main analysis centres around three case studies which occurred in Greenwich (London), Luton and Doncaster. In order to study ephemeral, outdoor arts events I developed a new, efficient and replicable methodology, which responds to their unique features and can evaluate their ability to intervene in everyday life and produce festivalized space. This uses multiple time-lapse cameras and an embedded, expert researcher to make detailed observations of the triad of core categories identified: space/time, performance and audience. This approach generated both quantitative and qualitative datasets: on the one hand delivering, for the first time, accurate numbers for audience engagements and empirical evidence of audience flows, stay-lengths and proxemics; and on the other hand, documenting atmospheres, connections and identity shifts. The thesis contributes to knowledge about the inspirations, motivations and priorities of those who create and produce outdoor arts today and their relationships to public space. It develops theory around the tendency for outdoor arts events to become more homogenous and increasingly codified in recent years, questioning the validity of the strategies and structures driving this process. It identifies and analyses a range of behaviours and techniques exclusively used by organisers, artists and audiences of outdoor arts and in doing s

[ES] Objetivo: Describir nuevas características de embriones capaces de predecir el potencial de implantación como datos de entrada para un modelo de red neuronal artificial (ANN). Diseño: Estudio de cohorte retrospectivo. Entorno: Centro de FIV privado afiliado a la universidad. Paciente (s): Este estudio incluyó a 637 pacientes del programa de donación de ovocitos que se sometieron a transferencia de un solo blastocisto durante dos años consecutivos. Intervención (es): Ninguna. Principales medidas de resultado: La investigación se dividió en dos fases. La fase 1 consistió en la descripción y análisis de las siguientes características embrionarias en embriones implantados y no implantados: distancia y velocidad de migración pronuclear, diámetro del blastocisto expandido, área de masa celular interna y duración del ciclo celular del trofoectodermo. La fase 2 consistió en el desarrollo de un algoritmo ANN para la predicción de la implantación. Se obtuvieron resultados para cuatro modelos alimentados con diferentes datos de entrada. El poder predictivo se midió con el uso del área bajo la curva característica operativa del receptor (AUC). Resultado (s): De los cinco nuevos parámetros descritos, el diámetro expandido del blastocisto y la duración del ciclo celular del trofoectodermo tenían valores estadísticamente diferentes en los embriones implantados y no implantados. Después de que los modelos ANN fueron entrenados y validados mediante validación cruzada cinco veces, estos fueron capaces de predecir la implantación en los datos de prueba con AUC de 0,64 para ANN1 (morfocinética convencional), 0,73 para ANN2 (morfodinámica novedosa), 0,77 para ANN3 (morfocinética convencional þ morfodinámica novedosa) y 0,68 para ANN4 (variables discriminatorias de prueba estadística). Conclusión (es): Las nuevas características embrionarias propuestas afectan al potencial de implantación y su combinación con parámetros morfocinéticos convencionales es eficaz como datos de entrada p, [EN] Objective: To describe novel embryo features capable of predicting implantation potential as input data for an artificial neural network (ANN) model. Design: Retrospective cohort study. Setting: University-affiliated private IVF center. Patient(s): This study included 637 patients from the oocyte donation program who underwent single-blastocyst transfer during two consecutive years. Intervention(s): None. Main Outcome Measure(s): The research was divided into two phases. Phase 1 consisted of the description and analysis of the following embryo features in implanted and nonimplanted embryos: distance and speed of pronuclear migration, blastocyst expanded diameter, inner cell mass area, and trophectoderm cell cycle length. Phase 2 consisted of the development of an ANN algorithm for implantation prediction. Results were obtained for four models fed with different input data. The predictive power was measured with the use of the area under the receiver operating characteristic curve (AUC). Result(s): Out of the five novel described parameters, blastocyst expanded diameter and trophectoderm cell cycle length had statistically different values in implanted and nonimplanted embryos. After the ANN models were trained and validated using fivefold cross validation, they were capable of predicting implantation on testing data with AUCs of 0.64 for ANN1 (conventional morphokinetics), 0.73 for ANN2 (novel morphodynamics), 0.77 for ANN3 (conventional morphokinetics thorn novel morphodynamics), and 0.68 for ANN4 (discriminatory variables from statistical test). Conclusion(s): The novel proposed embryo features affect the implantation potential, and their combination with conventional morphokinetic parameters is effective as input data for a predictive model based on artificial intelligence. ((c) 2020 by American Society for Reproductive Medicine.)

[ES] Objetivo: Describir nuevas características de embriones capaces de predecir el potencial de implantación como datos de entrada para un modelo de red neuronal artificial (ANN). Diseño: Estudio de cohorte retrospectivo. Entorno: Centro de FIV privado afiliado a la universidad. Paciente (s): Este estudio incluyó a 637 pacientes del programa de donación de ovocitos que se sometieron a transferencia de un solo blastocisto durante dos años consecutivos. Intervención (es): Ninguna. Principales medidas de resultado: La investigación se dividió en dos fases. La fase 1 consistió en la descripción y análisis de las siguientes características embrionarias en embriones implantados y no implantados: distancia y velocidad de migración pronuclear, diámetro del blastocisto expandido, área de masa celular interna y duración del ciclo celular del trofoectodermo. La fase 2 consistió en el desarrollo de un algoritmo ANN para la predicción de la implantación. Se obtuvieron resultados para cuatro modelos alimentados con diferentes datos de entrada. El poder predictivo se midió con el uso del área bajo la curva característica operativa del receptor (AUC). Resultado (s): De los cinco nuevos parámetros descritos, el diámetro expandido del blastocisto y la duración del ciclo celular del trofoectodermo tenían valores estadísticamente diferentes en los embriones implantados y no implantados. Después de que los modelos ANN fueron entrenados y validados mediante validación cruzada cinco veces, estos fueron capaces de predecir la implantación en los datos de prueba con AUC de 0,64 para ANN1 (morfocinética convencional), 0,73 para ANN2 (morfodinámica novedosa), 0,77 para ANN3 (morfocinética convencional þ morfodinámica novedosa) y 0,68 para ANN4 (variables discriminatorias de prueba estadística). Conclusión (es): Las nuevas características embrionarias propuestas afectan al potencial de implantación y su combinación con parámetros morfocinéticos convencionales es eficaz como datos de entrada p, [EN] Objective: To describe novel embryo features capable of predicting implantation potential as input data for an artificial neural network (ANN) model. Design: Retrospective cohort study. Setting: University-affiliated private IVF center. Patient(s): This study included 637 patients from the oocyte donation program who underwent single-blastocyst transfer during two consecutive years. Intervention(s): None. Main Outcome Measure(s): The research was divided into two phases. Phase 1 consisted of the description and analysis of the following embryo features in implanted and nonimplanted embryos: distance and speed of pronuclear migration, blastocyst expanded diameter, inner cell mass area, and trophectoderm cell cycle length. Phase 2 consisted of the development of an ANN algorithm for implantation prediction. Results were obtained for four models fed with different input data. The predictive power was measured with the use of the area under the receiver operating characteristic curve (AUC). Result(s): Out of the five novel described parameters, blastocyst expanded diameter and trophectoderm cell cycle length had statistically different values in implanted and nonimplanted embryos. After the ANN models were trained and validated using fivefold cross validation, they were capable of predicting implantation on testing data with AUCs of 0.64 for ANN1 (conventional morphokinetics), 0.73 for ANN2 (novel morphodynamics), 0.77 for ANN3 (conventional morphokinetics thorn novel morphodynamics), and 0.68 for ANN4 (discriminatory variables from statistical test). Conclusion(s): The novel proposed embryo features affect the implantation potential, and their combination with conventional morphokinetic parameters is effective as input data for a predictive model based on artificial intelligence. ((c) 2020 by American Society for Reproductive Medicine.)

© 2020 Wolters Kluwer Medknow Publications. All rights reserved. Objective: To investigate morphokinetic characteristics of embryos displaying either reverse cleavage or direct cleavage during the first, second or third cleavage cycle. Methods: A total of 167 in vitro fertilization and/or intracytoplasmic sperm injection treatment cycles undertaken by 167 women [aged (35.0±4.6) years] were included for reverse cleavage analysis, and a total of 167 in vitro fertilization and/or intracytoplasmic sperm injection treatment cycles undertaken by 167 women [aged (33.8 ±4.3) years] were included for direct cleavage analysis in this study. Using a sibling-embryo design, morphokinetic profiles (both before and after the onset of abnormal event) of embryos displaying reverse cleavage (n=241) or direct cleavage (n=244) were compared with their unaffected siblings (the controls) in the first, second and third cell cycles (n=32, n=142, n=562; n=195, n=412, n=205, respectively), at different developmental stages up to day 3. Results: Direct cleavage embryos demonstrated significantly delayed cleavage rates prior to the event regardless of developmental stage of the occurrence, while reverse cleavage embryos showed similar cleavage rates to their unaffected siblings. Post event, direct cleavage embryos sped up cleavage rates while reverse cleavage embryos slowed down. Conclusions: Altered morphokinetic profiles are displayed by direct cleavage embryos both before and after their occurrence and reverse cleavage embryos after the occurrence, which could potentially confound morphokinetic comparisons if not separated from their unaffected sibling embryos. Further study is warranted in order to fully understand the biological mechanisms of such events.

© 2020 Wolters Kluwer Medknow Publications. All rights reserved. Objective: To investigate morphokinetic characteristics of embryos displaying either reverse cleavage or direct cleavage during the first, second or third cleavage cycle. Methods: A total of 167 in vitro fertilization and/or intracytoplasmic sperm injection treatment cycles undertaken by 167 women [aged (35.0±4.6) years] were included for reverse cleavage analysis, and a total of 167 in vitro fertilization and/or intracytoplasmic sperm injection treatment cycles undertaken by 167 women [aged (33.8 ±4.3) years] were included for direct cleavage analysis in this study. Using a sibling-embryo design, morphokinetic profiles (both before and after the onset of abnormal event) of embryos displaying reverse cleavage (n=241) or direct cleavage (n=244) were compared with their unaffected siblings (the controls) in the first, second and third cell cycles (n=32, n=142, n=562; n=195, n=412, n=205, respectively), at different developmental stages up to day 3. Results: Direct cleavage embryos demonstrated significantly delayed cleavage rates prior to the event regardless of developmental stage of the occurrence, while reverse cleavage embryos showed similar cleavage rates to their unaffected siblings. Post event, direct cleavage embryos sped up cleavage rates while reverse cleavage embryos slowed down. Conclusions: Altered morphokinetic profiles are displayed by direct cleavage embryos both before and after their occurrence and reverse cleavage embryos after the occurrence, which could potentially confound morphokinetic comparisons if not separated from their unaffected sibling embryos. Further study is warranted in order to fully understand the biological mechanisms of such events.

The essay analyses the time-lapse videos connected to pregnancy, in the perspective of the presentation of self and one’s own identity on YouTube. The selected user-generated contents, also because of their peculiarity in terms of compression and time manipulation, represent the most visualised content of several channels dedicated to domestic vlogging. The observation of the phenomenon starts from a quantitative analysis whose purpose is to map the visual regime of the contents. Afterwards, a qualitative analysis has been carried out on a selected group of videos in order to define the generative process and the communicative coordinates on the rhetoric of the authenticity of YouTube, negotiated between public sphere and perception of private life. The data contributes to define the social dimension of the practices which were analysed, the homogenisation and the peculiar overview of similar videos in the widest dynamics of vlogging.

A new method is proposed to measure intergranular forces in granular geomaterial from time-lapsehigh-resolution X-ray computed tomographyimaging using a grain trackingapproachand discrete element method

Background : Chemotherapy-induced infertility has become problems faced by reproductive-age women receiving chemotherapy and many anticancer drugs mainly affect proliferating cells such as granulosa cells, which support the oocyte. The aims of this study were to investigate mitochondrial membrane potential and to test the protective effect of L-carnitine (LC) in the granulosa cells treated with anticancer drugs. / Methods : Female ICR mice were divided into 5 groups (control, cisplatin, cisplatin+LC, paclitaxel, paclitaxel+LC) and the number of follicles in three sections of ovaries was counted every 100 μm at each development stage....

type:Journal Article, Researchers have developed a cell stretching device to mimic the in vivo mechanical environment in vitro in order to investigate cell mechanotransduction. Cyclic stretch is involved in lengthening and relaxation phases. Cells may respond to mechanical stimulation rapidly within a few seconds, and sudden disruption of cell cytoskeletons may also occur at any point in any phase of cyclic stretch. However, until now, no research has been done to establish a method of collecting cell images at the two phases of cyclic stretch. Because image processing is time-consuming, it is difficult to adjust focus and collect high-resolution images simultaneously at the two phases during the process. In this study, a three-motorized-stage system was developed to meet the requirements. The results demonstrated that linear compensation is effective for cell imaging, and it is applicable to have a feed-forward control method without image processing. A method was then developed to determine the maximum displacement of the target in the horizontal and vertical directions, and the linear compensation waveforms were designed using the C program automatically and immediately before stretching. Further, the cyclic stretch was applied to cells using the three motorized stages, and clear phase-contrast cell imaging (30 fps) were obtained almost at any point in time. Detailed cell changes such as sudden disruption of cell–cell junctions, not only long-term cell response, were observed. Therefore, our study established a methodology to greatly improve the time resolution of imaging of cyclic stretch for the research of detailed cellular mechanotransduction., source:https://doi.org/10.3390/app9081560

In this retrospective study, patients undergoing ovarian stimulation were allocated into two groups (47 normal responders (NR) vs 47 low responders (LR)) according to the number of aspirated oocytes and their Anti-Müllerian hormone (AMH). 171 oocytes were retrieved from the LR group and 447 oocytes from the NR group. The oocytes were studied regarding the early embryo morphokinetic parameters and cleavage stage patterns after data extraction from our EmbryoScope database. The following parameters were recorded; time of PN fading (tPNf), time to two cells (t2), three cells (t3), four cells (t4), asynchrony in 2 nd cell cycle (S2), fragmentation, multinucleation, direct cleavages from 1 to 3 cells, reversed cleavage, rolling and non-tetrahedral shape. Except of an indicative time difference in t3 (dif = 0.884; p-value < 0.046), the differences in timings of early cell divisions were not statistically significant when tPNf was used as a starting point and the analysis was adjusted for age. No statistically significant differences were observed for irregular cleavage patterns, multinucleation at 2-cell stage, fragmentation and non-tetrahedral shape rate at the 4-cell stage. However, the risk for multinucleation at 4-cell stage is significantly lower in low responders (OR = 0.342; p-value < 0.019). The groups did not differ regarding fertilization and cleavage rates as well as the number of embryos that fulfill the European Society of Human Reproduction and Embryology (ESHRE) criteria for top quality characteristics on Day 2. Embryos derived from patients with low ovarian response have similar morphokinetic characteristics and cleavage stage pattern as embryos from patients with normal ovarian response.

I slutet av 1980-talet blev många fiskbestånd på svenska västkusten överfiskade vilket bl.a. resulterade i en minskning av kustnära bestånd av olika torskfiskarter. Havs- och vattenmyndigheten (tidigare Fiskeriverket) och Projekt 8-fjordar införde begränsningar i fisket 2010 för att skydda och få tillbaka de lokala bestånden av torskfisk i havsområdet innanför Orust och Tjörn, det så kallade 8-fjordarområdet. Fisket har varit hårt reglerat sen dess, men trots det återhämtar sig inte fiskebestånden. Det saknas vetenskaplig förklaring till varför bestånden av torskfiskar inte återhämtar sig, men de ökande populationerna av säl och skarv i Västerhavet föreslås emellanåt som en anledning. Sälpopulationen i Västerhavet har återhämtat sig från en tidigare låg nivå, orsakad av omfattande jakt och miljögifter, och antalet sälar befinner sig nu på en historiskt sett hög nivå. I 8-fjordarområdet har inga regelbundna inventeringar på sälar gjorts och hur många sälar som finns i området är idag okänt. För att ta reda på Hur mycket torsk som sälen egentligen äter i 8fjordar måste en uppskattning av antal sälar som befinner sig i området göras för att sedan kombineras med insamlade dietdata. Syftet med denna studie är att utvärdera metoden kameraövervakning med åtelkameror på två platser i 8-fjordarområdet där sälar brukar hålla till. I studien har ca 25 000 bilder analyserats. Det maximala antalet sälar som räknades under ett dygn på de två analyserade platserna var 39 stycken. Ifall fler platser skulle kunna hittas där sälar befinner sig regelbundet och i större antal (antal sälar > 10 på en bild) en bättre uppskattning göras och resultera i en mindre osäkerhet kring vilket antal sälar som lever innanför Orust fjordar. Vidare är placering av kameran en viktig del för att få bra bilder. Eftersom solen passerar i söder och risk för bländning uppstår periodvis då tidvattnet varierar bör kameran riktas mot nord, nordost eller nordväst. För att få bra överblick över sälarna är det b, In the late 1980’s there was a vast decrease of stocks of cod and other gadoid species on the west coast of Sweden due to overfishing. These fish stocks have not yet recovered. The National Board of Fisheries/Swedish Agency for Marine and Water Management and the 8-fjords initiative has since 2010 limited the fishing and introduced restricted rules in the fjords inside of Orust and Tjörn. The fishing of cod, pollack and haddock has been regulated since, but fish stocks have not recovered. Some scientists and fishermen consider a contributing factor to be the growing populations of harbor seals (Phoca vitulina) and great cormorants (Phalacrocorax carbo) in the area. The seal population has during the last decades varied due to diseases, but the trend has been increasing and the population has now recovered to a historically high level from previous low numbers. In order to understand the possible impact of seals on local fish stocks, an appreciation of the number of seals residing in the protected area was undertaken. Data from this study can later be combined with diet data to further investigate the consumption of cod. This essay aims to evaluate the method of using camera surveillance on two islands where harbour seals are known to haul out frequently. Approximately 25,000 images were analyzed. The maximum total number of seals counted in the same day but in two different areas was 39. However, if cameras were placed on additional islands where seals are known to reside (amount of seals > 10), the method of camera surveillance would be efficient to monitor the local abundance of seals in the area.

Objectives: The aim of this study was to assess different patterns of the human embryo secretome analysed as protein levels in culture media. Furthermore, analyses to correlate protein levels with quality and timing to development of human embryos were performed. Material and methods: Human day-2 cryopreserved embryos were cultured for four days in an EmbryoScope((R)) with a time-lapse camera, and embryo quality was evaluated retrospectively. After culture, the media were collected and relative levels of secreted proteins were analysed using Proseek Multiplex Assays. Protein levels were evaluated in relation to timing to development and the ability to form a blastocyst. Results: Specific patterns of timing of development of blastocysts were found, where a difference in time to start of cavitation was found between high- and low-quality blastocysts. There appeared to be a correlation between specific protein patterns and successful formation of morulae and blastocysts. Embryos developing into blastocysts had higher levels of EMMPRIN than arrested embryos, and levels of caspase-3 were lower in high- versus low-quality blastocysts. Also, higher levels of VEGF-A, IL-6, and EMMPRIN correlated with shorter times to morula formation. Conclusions: The secretome and timing to development differ in embryos forming blastocysts and those that become arrested, and in high- versus low-quality blastocysts. The levels of certain proteins also correlate to specific times to development.

Objectives: The aim of this study was to assess different patterns of the human embryo secretome analysed as protein levels in culture media. Furthermore, analyses to correlate protein levels with quality and timing to development of human embryos were performed. Material and methods: Human day-2 cryopreserved embryos were cultured for four days in an EmbryoScope((R)) with a time-lapse camera, and embryo quality was evaluated retrospectively. After culture, the media were collected and relative levels of secreted proteins were analysed using Proseek Multiplex Assays. Protein levels were evaluated in relation to timing to development and the ability to form a blastocyst. Results: Specific patterns of timing of development of blastocysts were found, where a difference in time to start of cavitation was found between high- and low-quality blastocysts. There appeared to be a correlation between specific protein patterns and successful formation of morulae and blastocysts. Embryos developing into blastocysts had higher levels of EMMPRIN than arrested embryos, and levels of caspase-3 were lower in high- versus low-quality blastocysts. Also, higher levels of VEGF-A, IL-6, and EMMPRIN correlated with shorter times to morula formation. Conclusions: The secretome and timing to development differ in embryos forming blastocysts and those that become arrested, and in high- versus low-quality blastocysts. The levels of certain proteins also correlate to specific times to development.

Objectives: The aim of this study was to assess different patterns of the human embryo secretome analysed as protein levels in culture media. Furthermore, analyses to correlate protein levels with quality and timing to development of human embryos were performed. Material and methods: Human day-2 cryopreserved embryos were cultured for four days in an EmbryoScope((R)) with a time-lapse camera, and embryo quality was evaluated retrospectively. After culture, the media were collected and relative levels of secreted proteins were analysed using Proseek Multiplex Assays. Protein levels were evaluated in relation to timing to development and the ability to form a blastocyst. Results: Specific patterns of timing of development of blastocysts were found, where a difference in time to start of cavitation was found between high- and low-quality blastocysts. There appeared to be a correlation between specific protein patterns and successful formation of morulae and blastocysts. Embryos developing into blastocysts had higher levels of EMMPRIN than arrested embryos, and levels of caspase-3 were lower in high- versus low-quality blastocysts. Also, higher levels of VEGF-A, IL-6, and EMMPRIN correlated with shorter times to morula formation. Conclusions: The secretome and timing to development differ in embryos forming blastocysts and those that become arrested, and in high- versus low-quality blastocysts. The levels of certain proteins also correlate to specific times to development.

AimsThis thesis includes three studies designed to investigate embryo selection in the IVF laboratory. Firstly, the agreement between embryologists when selecting a Day 5 embryo for transfer was evaluated. Following this, the use of time-lapse technology to improve embryo selection was assessed, and finally, the agreement between published time-lapse algorithms when selecting an embryo for transfer was investigated. MethodsIn the first study, ten embryologists morphologically graded and selected a single embryo for transfer using images of day 5 embryos. Inter- and intra-observer agreement was measured using the kappa coefficient. In the second study, time-lapse was used to record morphokinetic parameters of day 5 embryos and their ability to identify a top-quality blastocyst was assessed using logistic regression analyses. Finally, the agreement between published time-lapse algorithms when selecting a single day 5 embryo with the highest implantation potential was evaluated in the third study, as well as the agreement between these algorithms and experienced embryologists. ResultsThe agreement among embryologists in the first study when selecting a day 5 embryo for transfer was generally good. Assessment of agreement with regards to morphological grading was only fair to moderate. When time-lapse was investigated in the second study, eight parameters were found to predict top quality blastocyst formation. However, the resulting composite models had, at best, poor-to-moderate discriminatory capacity to predict blastocyst quality. In the third study, a highly variable agreement was found between published algorithms when selecting an embryo with the highest implantation potential. Moreover, the agreement between these algorithms and embryologists was poor to moderate. ConclusionsThe less than optimal agreement found between embryologists in the first study could negatively impact on pregnancy rates. However, development of a time-lapse model in the second study showe

In seismic monitoring, one is usually interested in the response of a changing target zone, embedded in a static inhomogeneous medium. We introduce an efficient method that predicts reflection responses at the Earth's surface for different target-zone scenarios, from a single reflection response at the surface and a model of the changing target zone. The proposed process consists of two main steps. In the first step, the response of the original target zone is removed from the reflection response, using the Marchenko method. In the second step, the modelled response of a new target zone is inserted between the overburden and underburden responses. The method fully accounts for all orders of multiple scattering and, in the elastodynamic case, for wave conversion. For monitoring purposes, only the second step needs to be repeated for each target-zone model. Since the target zone covers only a small part of the entire medium, the proposed method is much more efficient than repeated modelling of the entire reflection response., ImPhys/Acoustical Wavefield Imaging, Applied Geophysics and Petrophysics