English: A specific calcitic limestone source that is widely used in South African poultry diets was evaluated during two concurrent studies. During the first study, the effect of limestone particle size on in vivo and in vitro solubility, bone quality, egg production and eggshell quality was determined. Limestone was classified according to particle size as; small (0 − 1.0 mm), medium (1.0 − 2.0 mm) and large (2.0 − 3.8 mm). During the second study, the effect of different distribution ratios of small and large particle sizes of limestone on in vivo and in vitro solubility, bone quality, egg production and eggshell quality was determined. Small (0 − 1.0 mm) and large (2.0 − 3.8 mm) particles limestone from the first study was mixed to obtain the following five distribution ratios used in the second study namely; 0, 25, 50, 75, 100% small or large particles. The experimental protocol for both studies was the same. One hundred and ninety eight, 17 weeks old Lohmann-Silver pullets, were obtained from a commercial egg producer and randomly allocated to six treatments (n=33/treatment). All birds were kept in individual metabolic cages for the duration of the study. The influence of limestone particle size and distribution ratios of particles on feed intake, body weight and egg production was determined for weeks 19 to 32 as well as week 70 of age. During weeks 24, 28, 32 and 70 of age the effect of limestone particle size and distribution ratios of particles on eggshell quality characteristics such as shell weight, percentage eggshell, eggshell calcium, egg surface area, shell weight per unit surface area (SWUSA) and shell thickness was determined. The in vivo and in vitro limestone solubility of different limestone particles and the different distribution ratios of particles were determined during week 37 of age. At 37 and 70 weeks of age, the effect of limestone particle size and distribution ratios of limestone particles on bone dimensions (length, width and weight), Afrikaans: ‘n Spesifieke kalsitiese kalksteenbron wat algemeen in Suid-Afrika in pluimveerantsoene gebruik word, is tydens twee gelyklopende studies geëvalueer. Tydens die eerste studie is die invloed van partikelgrootte op die in vivo- en in vitro-kalksteenoplosbaarheid, beenkwaliteit, eierproduksie en eierdopkwaliteit bepaal. Die kalksteen is volgens partikelgrootte as fyn (0 − 1.0 mm), medium (1.0 − 2.0 mm) en grof (2.0 − 3.8 mm) geklassifiseer. Tydens die tweede studie is die invloed van partikelgrootte-verspreiding van fyn en growwe kalksteenpartikels op in vivo- en in vitro-oplosbaarheid, beenkwaliteit, eierproduksie en eierdopkwaliteit bepaal. Fyn (0 − 1.0 mm) en growwe (2.0 − 3.8 mm) kalksteenpartikels van die eerste studie is met mekaar gemeng om die volgende vyf partikelgrootte-verspreidings van 0, 25, 50, 75 en 100% fyn of growwe partikels te verkry. Die eksperimentele prosedure vir beide studies was dieselfde. Een honderd agt en negentig, Lohmann-Silver lêhenne (17 weke oud), is vanaf ‘n kommersiële eierprodusent verkry en ewekansig in ses behandelings ingedeel (n=33/behandeling). Die invloed van partikelgrootte en partikelgrootte-verspreiding op voerinhame, liggaamsgewig en eierproduksie vanaf week 19 tot 32 ouderdom asook gedurende week 70 is bepaal. Gedurende die ouderdom van week 24, 28, 32 en 70 is die invloed van partikelgrootte en partikelgrootte-verspreiding op eierdopkwaliteit-eienskappe soos dopgewig, persentasie dop, kalsiuminhoud, eieroppervlakte, eierdopgewig per eenheid oppervlakte en eierdopdikte bepaal. In vivo en in vitrokalksteenoplosbaarheid van die verskillende partikelgroottes en partikelgrootte-verspreidings is gedurende week 37 bepaal. Gedurende week 37 en 70 is die invloed van partikelgrootte en partikelgrootte-verspreiding op been-metings (lengte, dikte en gewig), meganiese eienskappe (sterkte en spanning) en persentasie been-as bepaal. Die resultate van die eerste studie toon dat ‘n toename in partikelgrootte van kalksteen met ‘, National Research Foundation (NRF)