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A menudo se relaciona el rol de desarrollador de videojuegos con unas excelentes prácticas de programación y optimización, dejando de lado el resto de los conocimientos que requiere un videojuego. Uno de los campos más ignorados por este perfil profesional es la computación gráfica y el funcionamiento de la tubería de renderizado, así como el desarrollo de shaders y efectos de posprocesado. Actualmente, esta muestra de desinterés de los programadores a menudo viene dada por entender dicha tecnología con fines únicamente artísticos, mientras que los artistas temen entrar en contacto con lenguaje de hardware a bajo nivel, quedándose en un espacio ambiguo entre ambos perfiles. El objetivo de este trabajo no se limita únicamente a desarrollar un videojuego con Unity o implementar shaders complejos e independientes, sino que ambas tareas deben convivir en una mecánica principal que guíe el diseño de niveles. Esto requiere de un ejercicio de imaginación que sea capaz de darnos una experiencia divertida y experimental, incapaz de replicarse sin recurrir a conocimientos avanzados de GPU o efectos ópticos en medios no lineales. Color Crisis presenta a un simpático guardia de seguridad de un museo que debe solventar el fatídico desastre que ha ocurrido la noche antes de su primer día de trabajo. Se adentrará en un mundo lleno de coloridos obstáculos, donde deberá usar correctamente su cubo de pintura para superar los puzles que se le presentan.

[Background]: The vasculature function is mainly regulated by the autonomic nervous system. Importantly, the sensory-motor nervous system also innervates peripheral vessels and has the capacity to modulate vascular tone. Here we investigated the effects of electrical stimulation of a mixed nerve trunk on blood flow in deep arteries and muscle perfusion. Our hypothesis is that stimulation of a mixed nerve can modify blood flow., [Methods]: Twenty-nine healthy participants were included into a randomized-crossover and blinded clinical trial. Each subject received a placebo and two percutaneous peripheral nerve stimulation (pPNS) protocols on the median nerve: Pain Threshold continuous Low Frequency (PT-cLF) and Sensory Threshold burst High Frequency (ST-bHF). Blood flow was then assessed bilaterally using Power Doppler Ultrasonography at the main arteries of the arm, and blood perfusion at the forearm muscles. Afterwards, blood flow was quantified using a semi-automatized software, freely shared here., [Results]: Placebo, consisting in needle insertion, produced an immediate and generalized reduction on peak systolic velocity in all arteries. Although nerve stimulation produced mainly no effects, some significant differences were found: both protocols increased the relative perfusion area of the forearm muscles, the ST-bHF protocol prevented the reduction in peak systolic velocity and TAMEAN of the radial artery produced by the control protocol and PT-cLF produced a TAMEAN reduction of the ulnar artery., [Conclusions]: Therefore, the arterial blood flow in the arm is mainly impervious to the electrical stimulation of the median nerve, composed by autonomic and sensory-motor axons, although it produces mild modifications in the forearm muscles perfusion.

Code Large Language Models (Code LLMs) have emerged as powerful tools, revolutionizing the software development landscape by automating the coding process and reducing time and effort required to build applications. This paper focuses on training Code LLMs to specialize in the field of quantum computing. We begin by discussing the unique needs of quantum computing programming, which differ significantly from classical programming approaches or languages. A Code LLM specializing in quantum computing requires a foundational understanding of quantum computing and quantum information theory. However, the scarcity of available quantum code examples and the rapidly evolving field, which necessitates continuous dataset updates, present significant challenges. Moreover, we discuss our work on training Code LLMs to produce high-quality quantum code using the Qiskit library. This work includes an examination of the various aspects of the LLMs used for training and the specific training conditions, as well as the results obtained with our current models. To evaluate our models, we have developed a custom benchmark, similar to HumanEval, which includes a set of tests specifically designed for the field of quantum computing programming using Qiskit. Our findings indicate that our model outperforms existing state-of-the-art models in quantum computing tasks. We also provide examples of code suggestions, comparing our model to other relevant code LLMs. Finally, we introduce a discussion on the potential benefits of Code LLMs for quantum computing computational scientists, researchers, and practitioners. We also explore various features and future work that could be relevant in this context.

Empleando herramientas como Blender para la creación e integración de entornos 3D y Unreal para su combinación con la entrada de vídeo del mundo real, se pretende conseguir una única señal de vídeo que permita experimentar la mayor sensación de realidad posible a partir de un plató creado artificialmente.

This explores the significant impact that Artificial Intelligence (AI) has had on the segmentation and targeting of political campaigns, real-world applications in the past, and trends for the immediate future. AI has revolutionized segmentation, allowing a micro-segmentation approach that divides the electorate into smaller groups based on individual behavior patterns, interests, and beliefs. Regarding targeting, AI has enhanced precision and efficiency, facilitating the prediction of electoral behavior and allowing A/B testing of different messages. However, this use of AI raises ethical and privacy challenges, including concerns about data protection and political manipulation. As technology advances, it is crucial for policymakers and legislators to keep pace to ensure the integrity and fairness of the electoral process., Se explora el significativo impacto que la Inteligencia Artificial (IA) ha tenido en la segmentación y el targeting de las campañas políticas, los casos reales de aplicación en el pasado y las tendencias que nos depara el futuro inmediato. La IA ha revolucionado la segmentación, permitiendo un enfoque de micro-segmentación que divide el electorado en grupos más pequeños basados en patrones de comportamiento, intereses y creencias individuales. En cuanto al targeting, la IA ha mejorado la precisión y la eficiencia, facilitando la predicción del comportamiento electoral y permitiendo pruebas A/B de diferentes mensajes. Sin embargo, este uso de la IA plantea desafíos éticos y de privacidad, incluyendo preocupaciones sobre la protección de datos y la manipulación política. A medida que la tecnología avanza, es crucial que los responsables políticos y los legisladores sigan el ritmo para garantizar la integridad y la justicia del proceso electoral.

Desde hace 8 años, el grado de Ingeniería Multimedia imparte el 4º curso utilizando Aprendizaje Basado en Proyectos (ABP) e integrando a todas las asignaturas en él. El programa ha tenido mucho éxito, pero siempre ha acusado un problema, la evaluación. Al tratarse de un trabajo en grupo altamente colaborativo, que integra a todas las asignaturas del curso y que durante los dos semestres trabaja en un único gran proyecto, es muy difícil discernir el trabajo real realizado por cada componente del equipo, produciéndose descompensaciones o incluso malas prácticas. Durante años, se ha tratado de adoptar medidas para paliar esta situación, pero persisten algunas disfunciones. Para solucionarlo, hemos diseñado e implantado una metodología de coevaluación, alineada con la gestión del programa ABP y que persigue dos objetivos: procurar un reparto de nota asociado con el esfuerzo individual realizado, basado en información objetiva y cuantificable, junto con una evaluación formativa y sumativa justa; y desarrollar las habilidades blandas imprescindibles hoy en día en entornos de trabajo colaborativos. En este artículo presentamos la herramienta de coevaluación desarrollada, la valoración realizada sobre la herramienta por las/os participantes en la experiencia y exalumnos de años anteriores, y los resultados obtenidos hasta la fecha. Esta herramienta se alinea perfectamente con la metodología docente. La herramienta hace partícipe al alumnado de su propia evaluación, pero también le hace más consciente de su trabajo frente al grupo. A través de ella, el profesorado puede conocer desde el principio si alguien no está trabajando adecuadamente, y además ese mismo estudiante también lo puede ver, lo que permite activar medidas correctivas y no sufrir las consecuencias al final, cuando se tensiona el grupo y los resultados ya no pueden recuperarse. La herramienta permite generar una evaluación continua real, iteración tras iteración, que el alumnado podrá utilizar como indicador d

Desde hace 8 años, el grado de Ingeniería Multimedia imparte el 4º curso utilizando Aprendizaje Basado en Proyectos (ABP) e integrando a todas las asignaturas en él. El programa ha tenido mucho éxito, pero siempre ha acusado un problema, la evaluación. Al tratarse de un trabajo en grupo altamente colaborativo, que integra a todas las asignaturas del curso y que durante los dos semestres trabaja en un único gran proyecto, es muy difícil discernir el trabajo real realizado por cada componente del equipo, produciéndose descompensaciones o incluso malas prácticas. Durante años, se ha tratado de adoptar medidas para paliar esta situación, pero persisten algunas disfunciones. Para solucionarlo, hemos diseñado e implantado una metodología de coevaluación, alineada con la gestión del programa ABP y que persigue dos objetivos: procurar un reparto de nota asociado con el esfuerzo individual realizado, basado en información objetiva y cuantificable, junto con una evaluación formativa y sumativa justa; y desarrollar las habilidades blandas imprescindibles hoy en día en entornos de trabajo colaborativos. En este artículo presentamos la herramienta de coevaluación desarrollada, la valoración realizada sobre la herramienta por las/os participantes en la experiencia y exalumnos de años anteriores, y los resultados obtenidos hasta la fecha., For the last 8 years, the Multimedia Engineering degree has been teaching the 4th year using Project Based Learning (PBL) and integrating all the subjects in it. The program has been very successful, but there has always been one problem: assessment. As it is a highly collaborative group work, integrating all the subjects of the course and working on a single large project during the two semesters, it is very difficult to discern the real work done by each member, causing imbalances or even malpractice. For years, we have tried to adopt measures to mitigate this situation, but without any positive results. To solve this problem, we have designed and implemented a co-evaluation methodology, aligned with the PBL program management, which has two objectives: to ensure a distribution of points associated with the individual effort, based on objective and measurable information, together with a fair formative and summative evaluation; and to develop the soft skills that are essential in current collaborative work environments. In this article we present the co-evaluation tool developed, the assessment made of the tool by the participants in the experience and alumni from previous years, and the results obtained to date.

[EN] This study was conducted to evaluate the antibacterial activity of two aminopeptidase inhibitors and chitosan-based nanoparticles in liquid-stored rabbit semen. This study reports that the aminopeptidase inhibitors used to prevent bacterial growth could be used in semen extender as a suitable alternative to antibiotics. Although great attention is paid to hygiene during semen collection and processing, bacteria are commonly found in the semen of healthy fertile males of different species. As the storage of extended semen might facilitate bacterial growth, extenders are commonly supplemented with antibiotics. This study aimed to evaluate the antibacterial activity of ethylenediaminetetraacetic acid (EDTA), bestatin and chitosan-based nanoparticles added to rabbit semen extender and their effect on reproductive performance under field conditions. Four different extenders were tested, supplemented with antibiotics (TCG+AB), with EDTA and bestatin (EB), with EDTA, bestatin and chitosan-based nanoparticles (QEB) or without antibiotics (TCG-AB). Extended semen was cooled at 15 degrees C for three days. Cooled samples were examined for bacterial growth and semen quality every 24 h for 3 days. The enterobacteria count increased considerably during storage at 72 h in semen extended with TCG+AB and TCG-AB, while extenders EB and QEB showed a bacteriostatic effect over time. After 24, 48 and 72 h, quality characteristics were retained in all groups, with no significant motility differences, either in acrosome integrity, membrane functionality or the viability of spermatozoa. Additionally, bacterial concentration present in fresh semen did not affect reproductive performance. In conclusion, EDTA and bestatin exerted a potent bacteriostatic effect over time and could be used as an alternative to conventional antibiotics in rabbit semen extenders.

[EN] This study was conducted to evaluate the antibacterial activity of two aminopeptidase inhibitors and chitosan-based nanoparticles in liquid-stored rabbit semen. This study reports that the aminopeptidase inhibitors used to prevent bacterial growth could be used in semen extender as a suitable alternative to antibiotics. Although great attention is paid to hygiene during semen collection and processing, bacteria are commonly found in the semen of healthy fertile males of different species. As the storage of extended semen might facilitate bacterial growth, extenders are commonly supplemented with antibiotics. This study aimed to evaluate the antibacterial activity of ethylenediaminetetraacetic acid (EDTA), bestatin and chitosan-based nanoparticles added to rabbit semen extender and their effect on reproductive performance under field conditions. Four different extenders were tested, supplemented with antibiotics (TCG+AB), with EDTA and bestatin (EB), with EDTA, bestatin and chitosan-based nanoparticles (QEB) or without antibiotics (TCG-AB). Extended semen was cooled at 15 degrees C for three days. Cooled samples were examined for bacterial growth and semen quality every 24 h for 3 days. The enterobacteria count increased considerably during storage at 72 h in semen extended with TCG+AB and TCG-AB, while extenders EB and QEB showed a bacteriostatic effect over time. After 24, 48 and 72 h, quality characteristics were retained in all groups, with no significant motility differences, either in acrosome integrity, membrane functionality or the viability of spermatozoa. Additionally, bacterial concentration present in fresh semen did not affect reproductive performance. In conclusion, EDTA and bestatin exerted a potent bacteriostatic effect over time and could be used as an alternative to conventional antibiotics in rabbit semen extenders.

La myxospermie et la myxocarpie (regroupées sous le terme myxodiasporie) désignent respectivement la capacité d'une graine ou d'un fruit à extruder du mucilage polysaccharidique lors de l'imbibition. Les espèces myxodiasporiques sont largement répandues parmi les plantes à fleurs, mais sont souvent étroitement apparentées aux espèces non myxodiasporiques. Cette répartition rend mystérieuse l'histoire évolutive de la myxodiasporie : Est-ce un trait ancestral qui s'est perdu plusieurs fois chez les espèces non myxodiasporiques? Ou est-ce une fonctionnalité qui est apparue plusieurs fois indépendamment chez les espèces myxodiasporiques? De plus, les rôles écologiques de la myxodiasporie sont très divers et semblent dépendre de l'espèce. La génétique contrôlant cette caractéristique est encore inégalement connue, en dehors de l'espèce modèle Arabidopsis thaliana. En effet, cette plante modèle est une Brassicaceae myxospermique qui a été bien caractérisée pour la morphologie, le développement, la composition et la génétique de son mucilage de graine et de ses cellules sécrétrices de mucilage (CSM). Cette thèse met en lumière l'évolution, la génétique et l'écologie de la myxospermie dans la famille des Brassicaceae au niveau inter- et intra-espèce. Au niveau inter-espèces, les graines et leurs cellules épidermiques d'une trentaine d'espèces de Brassicaceae ont été comparées à celles d'A. thaliana pour étudier l'évolution de la myxospermie dans cette famille. Chaque espèce de Brassicaceae a été soigneusement phénotypée à l'échelle macroscopique (graine entière) et microscopique (CSM) avant et après imbibition. De plus, les orthologues de chaque gène d'A. thaliana connus pour être impliqués dans la myxospermie ont été recherchés dans les génomes disponibles des espèces étudiées. La disponibilité de données transcriptomiques pour quelques espèces ont permis d'approfondir l'étude de ces gènes orthologues. Dans la famille des Brassicaceae, une grande diversité de structures de, The myxospermy and myxocarpy (grouped under the term myxodiaspory) designates the ability of a seed or a fruit to extrude mucilage upon imbibition, respectively. Myxodiasporous species are widely spread among flowering plants but are often closely related to non-myxodiasporous species. This repartition makes mysterious the evolutionary scenario of myxosdiaspory: Is it an ancestral trait which was lost several times in non-myxodiasporous species? Or is it a feature that appeared several times independently in myxodiasporous species? Additionally, the ecological roles of myxodiaspory are very diverse and seem to be species-dependent. Also, the genetics controlling this feature is still inequally known except for the model plant Arabidopsis thaliana. Indeed, this myxospermous Brassicaceae species has been well characterized for the morphology, development, composition, and genetics of its seed mucilage and mucilage secretory cells (MSCs). This thesis sheds light on the evolution, genetics, and ecology of myxospermy in the Brassicaceae family at both the inter- and intra- species level. At the inter-species level, the seeds and their epidermal cells from about thirty Brassicaceae species were compared to those of A. thaliana to investigate the myxospermy evolution in this family. Every Brassicaceae species was thoroughly phenotyped at the macroscopic (whole seed) and microscopic (MSCs) scale before and after imbibition. Additionally, ortholog from every A. thaliana gene known to be implicated in myxospermy was tracked in the available genomes of the studied species. The availability of transcriptomic data for few species allowed further investigation of these orthologous genes. Among the Brassicaceae family, a wide diversity of mucilage structures was observed at the macro- and microscopic level. Based on morphological data, the origin of myxospermy in Brassicaceae appears ancestral, but the trait is very different from that of A. thaliana. Despite the observed contrast

La myxospermie et la myxocarpie (regroupées sous le terme myxodiasporie) désignent respectivement la capacité d'une graine ou d'un fruit à extruder du mucilage polysaccharidique lors de l'imbibition. Les espèces myxodiasporiques sont largement répandues parmi les plantes à fleurs, mais sont souvent étroitement apparentées aux espèces non myxodiasporiques. Cette répartition rend mystérieuse l'histoire évolutive de la myxodiasporie : Est-ce un trait ancestral qui s'est perdu plusieurs fois chez les espèces non myxodiasporiques? Ou est-ce une fonctionnalité qui est apparue plusieurs fois indépendamment chez les espèces myxodiasporiques? De plus, les rôles écologiques de la myxodiasporie sont très divers et semblent dépendre de l'espèce. La génétique contrôlant cette caractéristique est encore inégalement connue, en dehors de l'espèce modèle Arabidopsis thaliana. En effet, cette plante modèle est une Brassicaceae myxospermique qui a été bien caractérisée pour la morphologie, le développement, la composition et la génétique de son mucilage de graine et de ses cellules sécrétrices de mucilage (CSM). Cette thèse met en lumière l'évolution, la génétique et l'écologie de la myxospermie dans la famille des Brassicaceae au niveau inter- et intra-espèce. Au niveau inter-espèces, les graines et leurs cellules épidermiques d'une trentaine d'espèces de Brassicaceae ont été comparées à celles d'A. thaliana pour étudier l'évolution de la myxospermie dans cette famille. Chaque espèce de Brassicaceae a été soigneusement phénotypée à l'échelle macroscopique (graine entière) et microscopique (CSM) avant et après imbibition. De plus, les orthologues de chaque gène d'A. thaliana connus pour être impliqués dans la myxospermie ont été recherchés dans les génomes disponibles des espèces étudiées. La disponibilité de données transcriptomiques pour quelques espèces ont permis d'approfondir l'étude de ces gènes orthologues. Dans la famille des Brassicaceae, une grande diversité de structures de, The myxospermy and myxocarpy (grouped under the term myxodiaspory) designates the ability of a seed or a fruit to extrude mucilage upon imbibition, respectively. Myxodiasporous species are widely spread among flowering plants but are often closely related to non-myxodiasporous species. This repartition makes mysterious the evolutionary scenario of myxosdiaspory: Is it an ancestral trait which was lost several times in non-myxodiasporous species? Or is it a feature that appeared several times independently in myxodiasporous species? Additionally, the ecological roles of myxodiaspory are very diverse and seem to be species-dependent. Also, the genetics controlling this feature is still inequally known except for the model plant Arabidopsis thaliana. Indeed, this myxospermous Brassicaceae species has been well characterized for the morphology, development, composition, and genetics of its seed mucilage and mucilage secretory cells (MSCs). This thesis sheds light on the evolution, genetics, and ecology of myxospermy in the Brassicaceae family at both the inter- and intra- species level. At the inter-species level, the seeds and their epidermal cells from about thirty Brassicaceae species were compared to those of A. thaliana to investigate the myxospermy evolution in this family. Every Brassicaceae species was thoroughly phenotyped at the macroscopic (whole seed) and microscopic (MSCs) scale before and after imbibition. Additionally, ortholog from every A. thaliana gene known to be implicated in myxospermy was tracked in the available genomes of the studied species. The availability of transcriptomic data for few species allowed further investigation of these orthologous genes. Among the Brassicaceae family, a wide diversity of mucilage structures was observed at the macro- and microscopic level. Based on morphological data, the origin of myxospermy in Brassicaceae appears ancestral, but the trait is very different from that of A. thaliana. Despite the observed contrast

En esta memoria se describe el proyecto de desarrollo de una metodología de Aprendizaje Basado en Proyectos (ABP) en su octava edición (curso 2020/21) para el itinerario de Creación y Entretenimiento digital del cuarto curso del Grado en Ingeniería Multimedia de la Escuela Politécnica Superior. La Red 4979 es el resultado de la consolidación de los realizados en los cursos anteriores, desde el curso 2013/14 hasta la actualidad (identificadores 3013, 3133, 3426, 3663, 4002, 4307 y 4606). En esta octava edición, el proyecto ha tenido como objetivo principal diseñar, implementar y evaluar algunas acciones educativas innovadoras respecto a la evaluación de los proyectos ABP. De esta forma, el objetivo de este proyecto es conseguir una evaluación multimodal, que valore todas las facetas que conforman una metodología como ésta: contenidos y objetivos, presentaciones de los proyectos, funcionamiento del grupo, resultado final del proyecto, … Para conseguir este objetivo tendrán que transcurrir algunos cursos en los que se irá trabajando en la implantación sucesiva de cambios puntuales y en la evaluación de sus resultados. En este trabajo se explica la primera aproximación realizada, y como resultados del proyecto se presentan una guía de buenas prácticas de evaluación y la valoración del alumnado.

The design and development of Serious Games is a complex task, including a considerable risk of failure. Many attempts end up in non-fun, non-engaging games that fail to meet the purpose of improving education. Many different proposals have been published in the form of design frameworks, with the aim of helping practitioners succeed. Although these frameworks define and explain relevant concepts and guidelines, there is lack of focus in iterative methodologies. These methodologies have proven valuable in other areas on engineering and are also used by commercial game designers. This work proposes the introduction of iterative design for Serious Games and presents an early stage methodology, along with an example of the core mechanic of a game and a prototype for learning the concept of slope of a line.

En esta memoria se describe el proyecto de desarrollo de una metodología de Aprendizaje Basado en Proyectos (ABP) en su octava edición (curso 2020/21) para el itinerario de Creación y Entretenimiento digital del cuarto curso del Grado en Ingeniería Multimedia de la Escuela Politécnica Superior. La Red 4979 es el resultado de la consolidación de los realizados en los cursos anteriores, desde el curso 2013/14 hasta la actualidad (identificadores 3013, 3133, 3426, 3663, 4002, 4307 y 4606). En esta octava edición, el proyecto ha tenido como objetivo principal diseñar, implementar y evaluar algunas acciones educativas innovadoras respecto a la evaluación de los proyectos ABP. De esta forma, el objetivo de este proyecto es conseguir una evaluación multimodal, que valore todas las facetas que conforman una metodología como ésta: contenidos y objetivos, presentaciones de los proyectos, funcionamiento del grupo, resultado final del proyecto, … Para conseguir este objetivo tendrán que transcurrir algunos cursos en los que se irá trabajando en la implantación sucesiva de cambios puntuales y en la evaluación de sus resultados. En este trabajo se explica la primera aproximación realizada, y como resultados del proyecto se presentan una guía de buenas prácticas de evaluación y la valoración del alumnado.

The design and development of Serious Games is a complex task, including a considerable risk of failure. Many attempts end up in non-fun, non-engaging games that fail to meet the purpose of improving education. Many different proposals have been published in the form of design frameworks, with the aim of helping practitioners succeed. Although these frameworks define and explain relevant concepts and guidelines, there is lack of focus in iterative methodologies. These methodologies have proven valuable in other areas on engineering and are also used by commercial game designers. This work proposes the introduction of iterative design for Serious Games and presents an early stage methodology, along with an example of the core mechanic of a game and a prototype for learning the concept of slope of a line.

[EN] Preimplantation embryo manipulations during standard assisted reproductive technologies (ART) have significant repercussions on offspring. However, few studies to date have investigated the potential long-term outcomes associated with the vitrification procedure. Here, we performed an experiment to unravel the particular effects related to stress induced by embryo transfer and vitrification techniques on offspring phenotype from the foetal period through to prepuberal age, using a rabbit model. In addition, the focus was extended to the liver function at prepuberal age. We showed that, compared to naturally conceived animals (NC), offspring derived after embryo exposure to the transfer procedure (FT) or cryopreservation-transfer procedure (VT) exhibited variation in growth and body weight from foetal life to prepuberal age. Strikingly, we found a nonlinear relationship between FT and VT stressors, most of which were already present in the FT animals. Furthermore, we displayed evidence of variation in liver function at prepuberal age, most of which occurred in both FT and VT animals. The present major novel finding includes a significant alteration of the steroid biosynthesis profile. In summary, here we provide that embryonic manipulation during the vitrification process is linked with embryo phenotypic adaptation detected from foetal life to prepuberal age and suggests that this phenotypic variation may be associated, to a great extent, with the effect of embryo transfer.

[EN] Males from a paternal line selected for growth traits were used to produce semen doses at insemination centres and farms in a breeding scheme for rabbit meat production. The aim of this study was to assess whether a program of selection by daily gain in fattening period changed the seminal traits, plasma and sperm proteome and the fertility of semen when used in artificial insemination. Thirty-nine males from a paternal line were obtained by re-derivation from vitrified embryos with a difference of 18 generations (G21V and G39V). Sperm production parameters, morphological traits, sperm motility parameters and viability were evaluated from ejaculates. Seminal plasma and sperm proteome of three pool ejaculates from 10 mature males of each group were analysed and semen doses were used to inseminate 311 females. Only the percentage of abnormal sperm showed significant differences, with G21V presenting fewer abnormal sperm than G39V (10.5 +/- 2.63 vs 23.8 +/- 1.98). The discriminant analysis (DA-PLS) showed a clear effect of the generation for plasma and sperm proteome. In seminal plasma, 643 proteins were reported and 64 proteins were differentially expressed, of which 56 were overexpressed in G39V (87.5%). Sperm proteome reported 1360 proteins with 132 differentially abundant proteins. Of the total, 89 proteins were overexpressed in G39V (67.4%). From the 64 and 132 differentially abundant proteins of plasma and sperm, 19 and 26 had a FC >1.5, 12 and 13 of them belonging to the Oryctolagus cuniculus taxonomy, respectively. Despite observing differences in important proteins related to capacitation, sperm motility or immunoprotection and consequently to the fertilization process (TMPRSS2, Serpin family, Farn71f1, ATPase H+ transporting accessory protein 2, carbonic anhydrase 2, UDP-glucose glycoprotein glucosyltransferase 2), no differences in fertility and prolificacy were detected when commercial seminal doses were used for insemination from both male groups. How

[EN] Simple Summary Assisted reproductive technologies (ARTs) involve an extraordinary change in the natural developmental trajectory of the mammalian embryo, incurring potential long-term and inheritable effects in the resulting offspring. The results of this study demonstrate, for the first time, that ex vivo embryo manipulations during fresh and vitrified embryo transfer are associated with paternally inherited bodyweight variation, but seemed not transmissible via the female germline. This asymmetry in the transmission of acquired features following ARTs suggests that embryo paternal and maternal genomes differ in their degree of susceptibility to the lasting effects of ARTs. This study would provide a novel view of developmental plasticity in the early mammalian embryo. The concept of developmental programming suggests that the early life environment influences offspring phenotype in later life, whose effects may also be manifested in further generations. Valuable pieces of evidence come from the fields applying assisted reproductive technologies (ARTs), which deprive embryos of their optimal maternal environment and were thus associated with subsequent developmental deviations. Recently, we demonstrated that the in vitro manipulations during a vitrified embryo transfer procedure incurs a cumulative and transgenerational decline in the growth performance of the resulting offspring. Here, we provide a longitudinal study to investigate whether previous developmental deviations could be indistinctly paternally or maternally transmitted using crossbred mattings. Our findings revealed that early embryo manipulations through fresh and vitrified embryo transfer incurred paternally transmissible effects over the growth pattern and adult body weight, which seemed not inheritable via the female germline. Similar inheritable effects were observed after fresh and vitrified embryo transfer, suggesting that disturbing optimal embryo development through in vitro manipulations

[EN] Simple Summary Assisted reproductive technologies (ARTs) involve an extraordinary change in the natural developmental trajectory of the mammalian embryo, incurring potential long-term and inheritable effects in the resulting offspring. The results of this study demonstrate, for the first time, that ex vivo embryo manipulations during fresh and vitrified embryo transfer are associated with paternally inherited bodyweight variation, but seemed not transmissible via the female germline. This asymmetry in the transmission of acquired features following ARTs suggests that embryo paternal and maternal genomes differ in their degree of susceptibility to the lasting effects of ARTs. This study would provide a novel view of developmental plasticity in the early mammalian embryo. The concept of developmental programming suggests that the early life environment influences offspring phenotype in later life, whose effects may also be manifested in further generations. Valuable pieces of evidence come from the fields applying assisted reproductive technologies (ARTs), which deprive embryos of their optimal maternal environment and were thus associated with subsequent developmental deviations. Recently, we demonstrated that the in vitro manipulations during a vitrified embryo transfer procedure incurs a cumulative and transgenerational decline in the growth performance of the resulting offspring. Here, we provide a longitudinal study to investigate whether previous developmental deviations could be indistinctly paternally or maternally transmitted using crossbred mattings. Our findings revealed that early embryo manipulations through fresh and vitrified embryo transfer incurred paternally transmissible effects over the growth pattern and adult body weight, which seemed not inheritable via the female germline. Similar inheritable effects were observed after fresh and vitrified embryo transfer, suggesting that disturbing optimal embryo development through in vitro manipulations

[EN] Males from a paternal line selected for growth traits were used to produce semen doses at insemination centres and farms in a breeding scheme for rabbit meat production. The aim of this study was to assess whether a program of selection by daily gain in fattening period changed the seminal traits, plasma and sperm proteome and the fertility of semen when used in artificial insemination. Thirty-nine males from a paternal line were obtained by re-derivation from vitrified embryos with a difference of 18 generations (G21V and G39V). Sperm production parameters, morphological traits, sperm motility parameters and viability were evaluated from ejaculates. Seminal plasma and sperm proteome of three pool ejaculates from 10 mature males of each group were analysed and semen doses were used to inseminate 311 females. Only the percentage of abnormal sperm showed significant differences, with G21V presenting fewer abnormal sperm than G39V (10.5 +/- 2.63 vs 23.8 +/- 1.98). The discriminant analysis (DA-PLS) showed a clear effect of the generation for plasma and sperm proteome. In seminal plasma, 643 proteins were reported and 64 proteins were differentially expressed, of which 56 were overexpressed in G39V (87.5%). Sperm proteome reported 1360 proteins with 132 differentially abundant proteins. Of the total, 89 proteins were overexpressed in G39V (67.4%). From the 64 and 132 differentially abundant proteins of plasma and sperm, 19 and 26 had a FC >1.5, 12 and 13 of them belonging to the Oryctolagus cuniculus taxonomy, respectively. Despite observing differences in important proteins related to capacitation, sperm motility or immunoprotection and consequently to the fertilization process (TMPRSS2, Serpin family, Farn71f1, ATPase H+ transporting accessory protein 2, carbonic anhydrase 2, UDP-glucose glycoprotein glucosyltransferase 2), no differences in fertility and prolificacy were detected when commercial seminal doses were used for insemination from both male groups. How

[EN] Preimplantation embryo manipulations during standard assisted reproductive technologies (ART) have significant repercussions on offspring. However, few studies to date have investigated the potential long-term outcomes associated with the vitrification procedure. Here, we performed an experiment to unravel the particular effects related to stress induced by embryo transfer and vitrification techniques on offspring phenotype from the foetal period through to prepuberal age, using a rabbit model. In addition, the focus was extended to the liver function at prepuberal age. We showed that, compared to naturally conceived animals (NC), offspring derived after embryo exposure to the transfer procedure (FT) or cryopreservation-transfer procedure (VT) exhibited variation in growth and body weight from foetal life to prepuberal age. Strikingly, we found a nonlinear relationship between FT and VT stressors, most of which were already present in the FT animals. Furthermore, we displayed evidence of variation in liver function at prepuberal age, most of which occurred in both FT and VT animals. The present major novel finding includes a significant alteration of the steroid biosynthesis profile. In summary, here we provide that embryonic manipulation during the vitrification process is linked with embryo phenotypic adaptation detected from foetal life to prepuberal age and suggests that this phenotypic variation may be associated, to a great extent, with the effect of embryo transfer.

Tesis por compendio, Los objetivos generales de esta tesis fueron desarrollar nuevos diluyentes de inseminación artificial (IA) suplementados con un análogo de GnRH y caracterizar el proteoma del semen de conejo. En el capítulo I, se evaluó la inclusión de un cóctel de inhibidores de proteasas en el diluyente de inseminación (DI) para evitar parte de la actividad proteasa del plasma seminal de conejo. La calidad seminal y la fertilidad no se vieron afectadas por el cóctel. Sin embargo, la prolificidad fue significativamente menor en el grupo experimental en comparación con los grupos de control positivo y negativo (8,2±0,22 vs. 9,3±0,23 y 9,2±0,26 gazapos por parto, respectivamente). De este capítulo, se puede concluir que la adición de una amplia variedad de inhibidores de proteasas en el diluyente de semen de conejo afecta negativamente la tasa de prolificidad y que en el futuro sería aconsejable probar inhibidores específicos de aminopeptidasas (AMIs). En el capítulo II, suplementamos el DI con AMIs (bestatina y EDTA), y estudiamos su efecto sobre la calidad seminal y el rendimiento reproductivo. La inclusión de AMIs no afectó la calidad seminal, ni la fertilidad (85,3 vs. 88,6%), ni la prolificidad (10,12 vs. 10,51 gazapos por parto) en comparación con el grupo control. Por lo tanto, concluimos que los AMIs se pueden utilizar en los DIs de conejo para inhibir parte de la actividad aminopeptidasa del plasma seminal (PS). En el capítulo III, probamos nuevos DIs de conejo que contenían AMIs con o sin nanopartículas de quitosano (CS)-sulfato de dextrano (DS) que atrapan el análogo de GnRH. Se estudiaron los siguientes diluyentes: C4 (4 µg de buserelina/coneja en medio control (MC): tris-ácido cítrico-glucosa suplementado con AMIs), C5 (5 µg de buserelina/coneja en MC), Q4 (4 µg de buserelina/coneja en nanopartículas CS-DS en MC) y grupo Q5 (5 µg de buserelina/coneja en nanopartículas CS-DS en MC). La fertilidad fue significativamente menor en el grupo C4 en comparación con los grupos C5, The general objectives of this thesis were to develop new artificial insemination (AI) extenders supplemented with a GnRH analogue and to characterise the proteomic profile of rabbit semen. In chapter I, the inclusion of a protease inhibitors cocktail in the insemination extender (IE) to avoid part of the rabbit seminal plasma protease activity was evaluated. Seminal quality and fertility rate were not affected by the cocktail, having similar values between experimental and control groups. However, prolificacy rate was significantly lower in experimental group compared to positive and negative control groups (8.2 ±0.22 vs. 9.3 ±0.23 and 9.2 ±0.26 total born per litter, respectively). From this chapter, it may be concluded that the addition of a wide variety of protease inhibitors in the rabbit semen extender negatively affects prolificacy rate and it in the future it would be advisable to test specific aminopeptidase inhibitors (AMIs). Therefore, in chapter II, we supplemented the IE with AMIs (bestatin and EDTA), and we studied their effect on rabbit seminal quality and reproductive performance. Seminal quality was not affected by AMIs. Regarding reproductive performance, the inclusion of AMIs, did not affect fertility (85.3 vs. 88.6%), nor the prolificacy rate (10.12 vs. 10.51 kits per delivery) in comparison with control group. Thus, we concluded that AMIs can be used in rabbit IEs to inhibit part of the seminal plasma aminopeptidase activity. In chapter III, we test new rabbit IEs containing AMIs with or without chitosan (CS)-dextran sulfate (DS) nanoparticles entrapping the GnRH analogue. The following experimental extenders were studied: C4 (4 µg buserelin/doe in control medium (CM): Tris-citric acid-glucose supplemented with AMIs), C5 (5 µg of buserelin/doe in CM), Q4 (4 µg of buserelin/doe into CS-DS nanoparticles in CM) and Q5 group (5 µg of busereline/doe into CS-DS nanoparticles in CM). Results showed that fertility was significantly lower in C4 group com, Els objectius generals d'aquesta tesi van ser desenvolupar nous diluents d'inseminació artificial (IA) suplementats amb un anàleg de GnRH i caracteritzar el proteoma del semen de conill. En el capítol I, es va avaluar la inclusió d'un còctel d'inhibidors de proteases en el diluent d'inseminació (DI) per evitar part de l'activitat proteasa del plasma seminal de conill. La qualitat seminal i la fertilitat no es van veure afectades pel còctel. No obstant això, la prolificitat va ser significativament menor en el grup experimental en comparació amb els grups de control positiu i negatiu (8,2 ± 0,22 vs. 9,3 ± 0,23 i 9,2 ± 0,26 catxaps per part, respectivament). D'aquest capítol, es pot concloure que l'addició d'una àmplia varietat d'inhibidors de proteases en el diluent de semen de conill afecta negativament la taxa de prolificitat i que en el futur seria aconsellable provar inhibidors específics de aminopeptidasas (AMIS). En el capítol II, suplementàrem el DI amb AMIs (bestatina i EDTA), i vam estudiar el seu efecte sobre la qualitat seminal i el rendiment reproductiu. La inclusió de AMIs no va afectar la qualitat seminal, ni la fertilitat (85,3 vs. 88,6%), ni la prolificitat (10,12 vs. 10,51 catxaps per part) en comparació amb el grup control. Per tant, concloem que els AMIs es poden utilitzar en els DIs de conill per inhibir part de l'activitat aminopeptidasa del plasma seminal (PS). En el capítol III, vam provar nous DIs de conill que contenien AMIs amb o sense nanopartícules de quitosà (CS)-sulfat de dextrà (DS) que atrapen l'anàleg de GnRH. Es van estudiar els següents diluents: C4 (4 µg de buserelina/conilla en medi control (MC): tris-àcid cítric-glucosa suplementat amb AMIs), C5 (5 µg de buserelina/conilla en MC), Q4 (4 µg de buserelina/conilla en nanopartícules CS-DS en MC) i grup Q5 (5 µg de buserelina/conilla en nanopartícules CS-DS en MC). La fertilitat va ser significativament menor en el grup C4 en comparació amb els grups C5, Q5 i Q4 (0,7 enfront de 0,85

Tesis por compendio, Los objetivos generales de esta tesis fueron desarrollar nuevos diluyentes de inseminación artificial (IA) suplementados con un análogo de GnRH y caracterizar el proteoma del semen de conejo. En el capítulo I, se evaluó la inclusión de un cóctel de inhibidores de proteasas en el diluyente de inseminación (DI) para evitar parte de la actividad proteasa del plasma seminal de conejo. La calidad seminal y la fertilidad no se vieron afectadas por el cóctel. Sin embargo, la prolificidad fue significativamente menor en el grupo experimental en comparación con los grupos de control positivo y negativo (8,2±0,22 vs. 9,3±0,23 y 9,2±0,26 gazapos por parto, respectivamente). De este capítulo, se puede concluir que la adición de una amplia variedad de inhibidores de proteasas en el diluyente de semen de conejo afecta negativamente la tasa de prolificidad y que en el futuro sería aconsejable probar inhibidores específicos de aminopeptidasas (AMIs). En el capítulo II, suplementamos el DI con AMIs (bestatina y EDTA), y estudiamos su efecto sobre la calidad seminal y el rendimiento reproductivo. La inclusión de AMIs no afectó la calidad seminal, ni la fertilidad (85,3 vs. 88,6%), ni la prolificidad (10,12 vs. 10,51 gazapos por parto) en comparación con el grupo control. Por lo tanto, concluimos que los AMIs se pueden utilizar en los DIs de conejo para inhibir parte de la actividad aminopeptidasa del plasma seminal (PS). En el capítulo III, probamos nuevos DIs de conejo que contenían AMIs con o sin nanopartículas de quitosano (CS)-sulfato de dextrano (DS) que atrapan el análogo de GnRH. Se estudiaron los siguientes diluyentes: C4 (4 µg de buserelina/coneja en medio control (MC): tris-ácido cítrico-glucosa suplementado con AMIs), C5 (5 µg de buserelina/coneja en MC), Q4 (4 µg de buserelina/coneja en nanopartículas CS-DS en MC) y grupo Q5 (5 µg de buserelina/coneja en nanopartículas CS-DS en MC). La fertilidad fue significativamente menor en el grupo C4 en comparación con los grupos C5, The general objectives of this thesis were to develop new artificial insemination (AI) extenders supplemented with a GnRH analogue and to characterise the proteomic profile of rabbit semen. In chapter I, the inclusion of a protease inhibitors cocktail in the insemination extender (IE) to avoid part of the rabbit seminal plasma protease activity was evaluated. Seminal quality and fertility rate were not affected by the cocktail, having similar values between experimental and control groups. However, prolificacy rate was significantly lower in experimental group compared to positive and negative control groups (8.2 ±0.22 vs. 9.3 ±0.23 and 9.2 ±0.26 total born per litter, respectively). From this chapter, it may be concluded that the addition of a wide variety of protease inhibitors in the rabbit semen extender negatively affects prolificacy rate and it in the future it would be advisable to test specific aminopeptidase inhibitors (AMIs). Therefore, in chapter II, we supplemented the IE with AMIs (bestatin and EDTA), and we studied their effect on rabbit seminal quality and reproductive performance. Seminal quality was not affected by AMIs. Regarding reproductive performance, the inclusion of AMIs, did not affect fertility (85.3 vs. 88.6%), nor the prolificacy rate (10.12 vs. 10.51 kits per delivery) in comparison with control group. Thus, we concluded that AMIs can be used in rabbit IEs to inhibit part of the seminal plasma aminopeptidase activity. In chapter III, we test new rabbit IEs containing AMIs with or without chitosan (CS)-dextran sulfate (DS) nanoparticles entrapping the GnRH analogue. The following experimental extenders were studied: C4 (4 µg buserelin/doe in control medium (CM): Tris-citric acid-glucose supplemented with AMIs), C5 (5 µg of buserelin/doe in CM), Q4 (4 µg of buserelin/doe into CS-DS nanoparticles in CM) and Q5 group (5 µg of busereline/doe into CS-DS nanoparticles in CM). Results showed that fertility was significantly lower in C4 group com, Els objectius generals d'aquesta tesi van ser desenvolupar nous diluents d'inseminació artificial (IA) suplementats amb un anàleg de GnRH i caracteritzar el proteoma del semen de conill. En el capítol I, es va avaluar la inclusió d'un còctel d'inhibidors de proteases en el diluent d'inseminació (DI) per evitar part de l'activitat proteasa del plasma seminal de conill. La qualitat seminal i la fertilitat no es van veure afectades pel còctel. No obstant això, la prolificitat va ser significativament menor en el grup experimental en comparació amb els grups de control positiu i negatiu (8,2 ± 0,22 vs. 9,3 ± 0,23 i 9,2 ± 0,26 catxaps per part, respectivament). D'aquest capítol, es pot concloure que l'addició d'una àmplia varietat d'inhibidors de proteases en el diluent de semen de conill afecta negativament la taxa de prolificitat i que en el futur seria aconsellable provar inhibidors específics de aminopeptidasas (AMIS). En el capítol II, suplementàrem el DI amb AMIs (bestatina i EDTA), i vam estudiar el seu efecte sobre la qualitat seminal i el rendiment reproductiu. La inclusió de AMIs no va afectar la qualitat seminal, ni la fertilitat (85,3 vs. 88,6%), ni la prolificitat (10,12 vs. 10,51 catxaps per part) en comparació amb el grup control. Per tant, concloem que els AMIs es poden utilitzar en els DIs de conill per inhibir part de l'activitat aminopeptidasa del plasma seminal (PS). En el capítol III, vam provar nous DIs de conill que contenien AMIs amb o sense nanopartícules de quitosà (CS)-sulfat de dextrà (DS) que atrapen l'anàleg de GnRH. Es van estudiar els següents diluents: C4 (4 µg de buserelina/conilla en medi control (MC): tris-àcid cítric-glucosa suplementat amb AMIs), C5 (5 µg de buserelina/conilla en MC), Q4 (4 µg de buserelina/conilla en nanopartícules CS-DS en MC) i grup Q5 (5 µg de buserelina/conilla en nanopartícules CS-DS en MC). La fertilitat va ser significativament menor en el grup C4 en comparació amb els grups C5, Q5 i Q4 (0,7 enfront de 0,85

El objetivo del proyecto es el desarrollo de un videojuego completo para dispositivos móviles Android e iOS utilizando el motor Unity. El proyecto abarcará todas las fases del desarrollo, desde el diseño hasta su publicación en las diferentes stores móviles. El videojuego será de tipo hyper-casual, siendo éste uno de los géneros de videojuegos más populares en dispositivos móviles, por tratarse de videojuegos con mecánicas sencillas fáciles de aprender, en los que se pueden jugar partidas rápidas.

House museums are a focus of tourist attraction that was born in Spain in the 19th century and developed during the 20th century. These museums respond to a very specific type of tourist and cultural demand that wants to satisfy the visitor's interest and curiosity regarding the private life of a public figure. They also contribute to the conservation and dissemination of collective memory and cultural heritage. In Spain, the Marquis de Vega-Inclán is recognized as a visionary museologist for being the creator of the first house museum: the El Greco house-museum in Toledo at the end of the 19th century. However, if we move to the Anglo-Saxon world we find that this model already existed previously. This is the case of Abbotsford, residence of Sir Walter Scott and also that of Shakespeare's birthplace in Stratford-upon-Avon, which becomes an international pilgrimage center after David Garrick's famous jubilee in 1769. The purpose of this article is to know the status of Shakespeare’s Birthplace as an international pilgrimage center at the end of the 19th century. For this we have the story that Benito Pérez Galdós made after his visit to Stratford-upon-Avon in 1889: Shakespeare's House. Galdós' narration informs us both of the visitors who frequented the birthplace of Shakespeare and the hotel industry that emerged around this museum, offering us his vision of how tourism transformed the economy of this English city., Las casas-museo son un foco de atracción turística que en España nace en el siglo XIX y se desarrolla durante el siglo XX. Estos museos dan respuesta a un tipo de demanda turística y cultural muy concreta que quiere satisfacer el interés y curiosidad del visitante relativa a la vida privada de un personaje público. Además contribuyen a la conservación y difusión de la memoria colectiva y del patrimonio cultural. En España, el marqués de Vega-Inclán es reconocido como un museólogo visionario por ser el creador de la primera casa museo: la casa-museo de El Greco en Toledo a finales del siglo XIX. Sin embargo, si nos trasladamos al mundo anglosajón encontramos que este modelo ya existía anteriormente. Es el caso de Abbotsford, residencia de Sir Walter Scott y también el de la casa natal de Shakespeare en Stratford-upon-Avon que se convierte en un centro de peregrinación internacional tras el famoso jubileo de David Garrick en 1769. El objeto de esta artículo consiste en conocer el estado del Shakespeare’s Birthplace, la casa natal de Shakespeare, como centro de peregrinaje internacional a finales del siglo XIX. Para ello contamos con el relato que Benito Pérez Galdós realizó después de su visita a Stratford en 1889: La casa de Shakespeare. La narración de Galdós nos informa de los visitantes que frecuentaron la casa natal de Shakespeare y de la industria hotelera que surgió alrededor de este museo ofreciéndosenos su visión de cómo el turismo transformó la economía de esta ciudad inglesa.

House museums are a focus of tourist attraction that was born in Spain in the 19th century and developed during the 20th century. These museums respond to a very specific type of tourist and cultural demand that wants to satisfy the visitor's interest and curiosity regarding the private life of a public figure. They also contribute to the conservation and dissemination of collective memory and cultural heritage. In Spain, the Marquis de Vega-Inclán is recognized as a visionary museologist for being the creator of the first house museum: the El Greco house-museum in Toledo at the end of the 19th century. However, if we move to the Anglo-Saxon world we find that this model already existed previously. This is the case of Abbotsford, residence of Sir Walter Scott and also that of Shakespeare's birthplace in Stratford-upon-Avon, which becomes an international pilgrimage center after David Garrick's famous jubilee in 1769. The purpose of this article is to know the status of Shakespeare’s Birthplace as an international pilgrimage center at the end of the 19th century. For this we have the story that Benito Pérez Galdós made after his visit to Stratford-upon-Avon in 1889: Shakespeare's House. Galdós' narration informs us both of the visitors who frequented the birthplace of Shakespeare and the hotel industry that emerged around this museum, offering us his vision of how tourism transformed the economy of this English city., Las casas-museo son un foco de atracción turística que en España nace en el siglo XIX y se desarrolla durante el siglo XX. Estos museos dan respuesta a un tipo de demanda turística y cultural muy concreta que quiere satisfacer el interés y curiosidad del visitante relativa a la vida privada de un personaje público. Además contribuyen a la conservación y difusión de la memoria colectiva y del patrimonio cultural. En España, el marqués de Vega-Inclán es reconocido como un museólogo visionario por ser el creador de la primera casa museo: la casa-museo de El Greco en Toledo a finales del siglo XIX. Sin embargo, si nos trasladamos al mundo anglosajón encontramos que este modelo ya existía anteriormente. Es el caso de Abbotsford, residencia de Sir Walter Scott y también el de la casa natal de Shakespeare en Stratford-upon-Avon que se convierte en un centro de peregrinación internacional tras el famoso jubileo de David Garrick en 1769. El objeto de esta artículo consiste en conocer el estado del Shakespeare’s Birthplace, la casa natal de Shakespeare, como centro de peregrinaje internacional a finales del siglo XIX. Para ello contamos con el relato que Benito Pérez Galdós realizó después de su visita a Stratford en 1889: La casa de Shakespeare. La narración de Galdós nos informa de los visitantes que frecuentaron la casa natal de Shakespeare y de la industria hotelera que surgió alrededor de este museo ofreciéndosenos su visión de cómo el turismo transformó la economía de esta ciudad inglesa.

El objetivo del proyecto es el desarrollo de un videojuego completo para dispositivos móviles Android e iOS utilizando el motor Unity. El proyecto abarcará todas las fases del desarrollo, desde el diseño hasta su publicación en las diferentes stores móviles. El videojuego será de tipo hyper-casual, siendo éste uno de los géneros de videojuegos más populares en dispositivos móviles, por tratarse de videojuegos con mecánicas sencillas fáciles de aprender, en los que se pueden jugar partidas rápidas.

[EN] Contents Superovulation protocols are designed to achieve maximum embryo yields. Nevertheless, ovarian response control and the quality of obtained embryos are still a challenge. On the other hand, to save the superovulated embryos until their subsequent use, it is usual to cryopreserve them, so it is also crucial to assess their cryotolerance. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (FSH-CTP) alone or supplemented with human chorionic gonadotropin (hCG) and to determine the impact of this stimulation on in vitro and in vivo development of fresh or devitrified embryos. Our outcomes showed that ovulation rate and recovered embryos were significantly increased when hCG was used. In vitro development of fresh and devitrified embryos and survival at birth were not significantly affected by superstimulation treatment. Results of this study suggest that a single injection of long-acting FSH-CTP supplemented with hCG can be effectively used in rabbits to elicit an increase in ovulation rate and number of recovered embryos. Furthermore, we demonstrated that hCG supplementation had no negative effects in embryo cryosurvival and development, showing similar survival rate at birth than FSH-CTP alone group.

[EN] Contents Superovulation protocols are designed to achieve maximum embryo yields. Nevertheless, ovarian response control and the quality of obtained embryos are still a challenge. On the other hand, to save the superovulated embryos until their subsequent use, it is usual to cryopreserve them, so it is also crucial to assess their cryotolerance. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (FSH-CTP) alone or supplemented with human chorionic gonadotropin (hCG) and to determine the impact of this stimulation on in vitro and in vivo development of fresh or devitrified embryos. Our outcomes showed that ovulation rate and recovered embryos were significantly increased when hCG was used. In vitro development of fresh and devitrified embryos and survival at birth were not significantly affected by superstimulation treatment. Results of this study suggest that a single injection of long-acting FSH-CTP supplemented with hCG can be effectively used in rabbits to elicit an increase in ovulation rate and number of recovered embryos. Furthermore, we demonstrated that hCG supplementation had no negative effects in embryo cryosurvival and development, showing similar survival rate at birth than FSH-CTP alone group.

Political communication has been transformed by digital media. Digital media empower citizens: now, they decide on what they watch. Political parties should be aware of this fact, replacing marketing-based messages with an honest communication linked to people’s concerns. Taking this into account: how can we elaborate a proper digital communication strategy? In order to get an answer, we will analyse several strategy’s components: goals, audience, message and format, channel and digital platform, timeline and budget and technology., Los medios de comunicación digitales han cambiado la comunicación política. En este formato comunicativo, son los ciudadanos quienes tienen el poder de escoger lo que ven y lo que desechan, obligando a los partidos a transitar de la mera comunicación publicitaria a la comunicación nacida de la honestidad y de la preocupación por los problemas de la gente. Partiendo de aquí, pretendemos centrarnos en una cuestión concreta: ¿Cómo elaborar una estrategia de comunicación digital adecuada? Para responder a ella, analizaremos los distintos elementos de los que esta estrategia se compone: objetivos, audiencia, mensaje y formato, canales y plataformas digitales, cronograma y presupuesto y tecnología.

[EN] Assisted reproductive techniques (ARTs), such as in vitro embryo culture or embryo cryopreservation, affect natural development patterns with perinatal and postnatal consequences. To ensure the innocuousness of ART applications, studies in animal models are necessary. In addition, as a last step, embryo development studies require evaluation of their capacity to develop full-term healthy offspring. Here, embryo transfer to the uterus is indispensable to perform any ARTs-related experiment. The rabbit has been used as a model organism to study mammalian reproduction for over a century. In addition to its phylogenetic proximity to the human species and its small size and low maintenance cost, it has important reproductive characteristics such as induced ovulation, a chronology of early embryonic development similar to humans and a short gestation that allow us to study the consequences of ART application easily. Moreover, ARTs (such as intracytoplasmic sperm injection, embryo culture, or cryopreservation) are applied with suitable efficiency in this species. Using the laparoscopic embryo transfer technique and the cryopreservation protocol presented in this article, we describe 1) how to transfer embryos through an easy, minimally invasive technique and 2) an effective protocol for long-term storage of rabbit embryos to provide time- flexible logistical capacities and the ability to transport the sample. The outcomes obtained after transferring rabbit embryos at different developmental stages indicate that morula is the ideal stage for rabbit embryo recovery and transfer. Thus, an oviductal embryo transfer is required, justifying the surgical procedure. Furthermore, rabbit morulae are successfully vitrified and laparoscopically transferred, proving the effectiveness of the described techniques.

[EN] Assisted reproductive techniques (ARTs), such as in vitro embryo culture or embryo cryopreservation, affect natural development patterns with perinatal and postnatal consequences. To ensure the innocuousness of ART applications, studies in animal models are necessary. In addition, as a last step, embryo development studies require evaluation of their capacity to develop full-term healthy offspring. Here, embryo transfer to the uterus is indispensable to perform any ARTs-related experiment. The rabbit has been used as a model organism to study mammalian reproduction for over a century. In addition to its phylogenetic proximity to the human species and its small size and low maintenance cost, it has important reproductive characteristics such as induced ovulation, a chronology of early embryonic development similar to humans and a short gestation that allow us to study the consequences of ART application easily. Moreover, ARTs (such as intracytoplasmic sperm injection, embryo culture, or cryopreservation) are applied with suitable efficiency in this species. Using the laparoscopic embryo transfer technique and the cryopreservation protocol presented in this article, we describe 1) how to transfer embryos through an easy, minimally invasive technique and 2) an effective protocol for long-term storage of rabbit embryos to provide time- flexible logistical capacities and the ability to transport the sample. The outcomes obtained after transferring rabbit embryos at different developmental stages indicate that morula is the ideal stage for rabbit embryo recovery and transfer. Thus, an oviductal embryo transfer is required, justifying the surgical procedure. Furthermore, rabbit morulae are successfully vitrified and laparoscopically transferred, proving the effectiveness of the described techniques.

[EN] The present study was conducted to characterise rabbit seminal plasma proteins (SP proteins) focusing on the influence of the genetic origin and seasonality. In addition, ß-NGF protein quantity in SP was determined. Semen samples were recovered from January to December 2014 using 6 males belonging to genotype A and six from genotype R. For each genotype, one pooled sample at the beginning, middle and end of each season was selected to develop the experiment. A total of 24 pools (3 for each season and genetic line) were analysed. SP proteins of the two experimental groups were recovered and subjected to in-solution digestion nano LC¿MS/MS and bioinformatics analysis. The resulting library included 402 identified proteins validated with ¿95% Confidence (unused Score¿1.3). These data are available via ProteomeXchange with identifier PXD006308. Only 6 proteins were specifically implicated in reproductive processes according to Gene Ontology annotation. Twenty-three proteins were differentially expressed between genotypes, 11 over-expressed in genotype A and 12 in genotype R. Regarding the effect of season on rabbit SP proteome, results showed that there is no clear pattern of protein variation throughout the year. Similar ß-NGF relative quantity was observed between seasons and genotypes. In conclusion, this study generates the largest library of SP proteins reported to date in rabbits and provides evidence that genotype is related to a specific abundance of SP proteins.

[EN] The addition of aminopeptidase inhibitors (AMIs) to rabbit semen extenders could be a solution to decrease the hormone degradation (GnRH) by the aminopeptidases existing in the seminal plasma. Therefore, the quantity of GnRH needed to induce ovulation in doe would be comparable with the amount administered intramuscularly (i.m.). This study was conducted to evaluate the effects of two AMIs (bestatin and EDTA) on rabbit semen quality parameters, beta nerve growth factor ((beta-NGF) degradation and reproductive performance after artificial insemination. Results showed that seminal quality was not affected by the incubation with AMIs; the values of motility, acrosome integrity and sperm viability were not significantly different between the AMIs and the control groups (positive i.m. and negative intravaginally without AMIs). In addition, the aminopeptidase activity of seminal plasma was inhibited in a 55.5% by the AMIs as well as beta-NGF degradation. On the other hand, regarding the effect of AMIs on reproductive performance, our results showed that the presence of bestatin and EDTA did neither affect fertility (85.3 vs. 88.6%), nor the prolificacy rate (10.12 vs. 10.51 kits per delivery), comparing AMIs group to positive control group, respectively. We conclude that the addition of specific AMIs in the rabbit semen extender has no effect on reproductive performance. Therefore, due to the fact that AMIs inhibit part of the aminopeptidase activity that degrades the GnRH analogue and beta-NGF, they could be used to develop new extenders with less hormone concentration. (C) 2017 Elsevier Inc. All rights reserved.

[EN] The efficiency of an embryo bank depends on provision of optimal conditions for recovery, cryopreservation and transfer to a breed or strain. In this sense, increasing the number of embryos available using superovulation should improve the cryobank efficiency. However, vagueness of response to conventional protocols to control or increase ovarian response and the quality of oocytes and embryos and their cryotolerance remain a challenge. The aim of our study was to evaluate the effect of corifollitropin alpha (CTP) and a recombinant human FSH (rhFSH), alone or supplemented with rhLH, on embryo cryosurvival by in vitro development and OCT4 and NANOG mRNA abundance at blastocyst stage and offspring rate. In vitro development of vitrified embryos was not significantly affected by superstimulation with or without rhLH supplementation, resulting in similar development rates to those of the control groups (fresh and vitrified embryos from non-superstimulated donor does). Blastocysts developed from vitrified embryos showed higher levels of OCT4 transcript abundance than fresh control, while NANOG transcript abundance was only higher in the blastocysts developed from vitrified embryos after superstimulation treatment in comparison with control groups. The implantation and offspring rates at birth were negatively affected by supplementation with rhLH. Both rhFSH or CTP vitrified embryo groups showed an implantation rate similar to those of the control groups, but an offspring rate lower than control. In conclusion, embryos produced using corifollitropin alpha did not compromise the cryosurvival of vitrified embryos in the rabbit. In addition, this study points out the negative effect of rhLH supplementation in terms of offspring rate on embryo vitrification.

[EN] The present study was conducted to characterise rabbit seminal plasma proteins (SP proteins) focusing on the influence of the genetic origin and seasonality. In addition, ß-NGF protein quantity in SP was determined. Semen samples were recovered from January to December 2014 using 6 males belonging to genotype A and six from genotype R. For each genotype, one pooled sample at the beginning, middle and end of each season was selected to develop the experiment. A total of 24 pools (3 for each season and genetic line) were analysed. SP proteins of the two experimental groups were recovered and subjected to in-solution digestion nano LC¿MS/MS and bioinformatics analysis. The resulting library included 402 identified proteins validated with ¿95% Confidence (unused Score¿1.3). These data are available via ProteomeXchange with identifier PXD006308. Only 6 proteins were specifically implicated in reproductive processes according to Gene Ontology annotation. Twenty-three proteins were differentially expressed between genotypes, 11 over-expressed in genotype A and 12 in genotype R. Regarding the effect of season on rabbit SP proteome, results showed that there is no clear pattern of protein variation throughout the year. Similar ß-NGF relative quantity was observed between seasons and genotypes. In conclusion, this study generates the largest library of SP proteins reported to date in rabbits and provides evidence that genotype is related to a specific abundance of SP proteins.

[EN] The addition of aminopeptidase inhibitors (AMIs) to rabbit semen extenders could be a solution to decrease the hormone degradation (GnRH) by the aminopeptidases existing in the seminal plasma. Therefore, the quantity of GnRH needed to induce ovulation in doe would be comparable with the amount administered intramuscularly (i.m.). This study was conducted to evaluate the effects of two AMIs (bestatin and EDTA) on rabbit semen quality parameters, beta nerve growth factor ((beta-NGF) degradation and reproductive performance after artificial insemination. Results showed that seminal quality was not affected by the incubation with AMIs; the values of motility, acrosome integrity and sperm viability were not significantly different between the AMIs and the control groups (positive i.m. and negative intravaginally without AMIs). In addition, the aminopeptidase activity of seminal plasma was inhibited in a 55.5% by the AMIs as well as beta-NGF degradation. On the other hand, regarding the effect of AMIs on reproductive performance, our results showed that the presence of bestatin and EDTA did neither affect fertility (85.3 vs. 88.6%), nor the prolificacy rate (10.12 vs. 10.51 kits per delivery), comparing AMIs group to positive control group, respectively. We conclude that the addition of specific AMIs in the rabbit semen extender has no effect on reproductive performance. Therefore, due to the fact that AMIs inhibit part of the aminopeptidase activity that degrades the GnRH analogue and beta-NGF, they could be used to develop new extenders with less hormone concentration. (C) 2017 Elsevier Inc. All rights reserved.

[EN] The efficiency of an embryo bank depends on provision of optimal conditions for recovery, cryopreservation and transfer to a breed or strain. In this sense, increasing the number of embryos available using superovulation should improve the cryobank efficiency. However, vagueness of response to conventional protocols to control or increase ovarian response and the quality of oocytes and embryos and their cryotolerance remain a challenge. The aim of our study was to evaluate the effect of corifollitropin alpha (CTP) and a recombinant human FSH (rhFSH), alone or supplemented with rhLH, on embryo cryosurvival by in vitro development and OCT4 and NANOG mRNA abundance at blastocyst stage and offspring rate. In vitro development of vitrified embryos was not significantly affected by superstimulation with or without rhLH supplementation, resulting in similar development rates to those of the control groups (fresh and vitrified embryos from non-superstimulated donor does). Blastocysts developed from vitrified embryos showed higher levels of OCT4 transcript abundance than fresh control, while NANOG transcript abundance was only higher in the blastocysts developed from vitrified embryos after superstimulation treatment in comparison with control groups. The implantation and offspring rates at birth were negatively affected by supplementation with rhLH. Both rhFSH or CTP vitrified embryo groups showed an implantation rate similar to those of the control groups, but an offspring rate lower than control. In conclusion, embryos produced using corifollitropin alpha did not compromise the cryosurvival of vitrified embryos in the rabbit. In addition, this study points out the negative effect of rhLH supplementation in terms of offspring rate on embryo vitrification.

Storage of rabbit semen for longer than 36 h causes deterioration of semen quality. Therefore, the aim of this study was to evaluate the addition of antioxidants, membrane protective and/or aminopeptidase activity substances to an EDTA supplemented extender on rabbit semen quality preserved at 16ºC during 72 h. Experiment one was carried out to evaluate the effect of the inclusion of two water soluble analogs of vitamin E (Trolox and ¿-tocopherol) and / or dextran on spermatozoa motility, viability, acrosome status and membrane functionality. In a second experiment the inclusion of bestatin in the extenders with Trolox and / or dextran on seminal quality and lipid oxidation was evaluated. The presence of ¿-tocopherol in the extender significantly decreased all seminal quality parameters over time, whereas the presence of Trolox and dextran did not affect them. In the second experiment, no significant differences among the experimental extenders were observed either for the seminal quality parameters or for the lipid oxidation of the seminal plasma at 72 hours of refrigeration. The cooling time significantly affected the viability and integrity of the acrosome (P <0.05), however, no significant differences were observed in the kinetic parameters, membrane functionality and lipid oxidation of the seminal plasma, showing at 72 and 0 hours similar values. The inclusion of bestatin in all extenders had a positive effect on semen conservation, maintaining the semen characteristics and lipid oxidation over time, regardless of whether the extender was supplemented or not with dextran or Trolox. In conclusion, the combination of EDTA and bestatin proved to be a suitable alternative in the refrigerated semen extender since it avoids the antioxidant activity and maintains membrane motility and functionality over the cooling period studied., El semen de conejo puede ser conservado en refrigeración un corto periodo de tiempo antes de que se reduzca o pierda su capacidad fecundante, por lo que no suele utilizarse en inseminación artificial más allá de 36 horas desde su extracción. Por ello el objetivo del presente estudio fue estudiar el efecto de la suplementación de sustancias con actividad protectora de membrana, antioxidante y antiproteasa a un diluyente con EDTA sobre la calidad espermática y la oxidación lipídica del plasma seminal del semen refrigerado a 16ºC durante 72 horas. En un primer experimento se evaluó el efecto de la inclusión de dos análogos hidrosolubles de la vitamina E (Trolox y ¿-tocoferol) y/o el dextrano sobre los parámetros de calidad seminal: motilidad, viabilidad, estado del acrosoma y funcionalidad de la membrana. En un segundo experimento se evaluó la inclusión de la bestatina en los diluyentes con Trolox y/o dextrano sobre la calidad seminal y la oxidación de lípidos. La inclusión de ¿-tocoferol en el diluyente disminuyó significativamente los valores de todos los parámetros de calidad seminal a lo largo del tiempo, mientras que la presencia de Trolox y dextrano no afectó a las mismas. En el segundo experimento el diluyente utilizado no afectó significativamente (P>0,05) a los parámetros de calidad seminal ni a la oxidación lipídica del plasma seminal a las 72 horas de refrigeración. El tiempo de refrigeración afectó significativamente a la viabilidad y a la integridad del acrosoma (P<0,05), sin embargo no se observaron diferencias significativas en los parámetros cinéticos, de funcionalidad de membrana y de oxidación lipídica del plasma seminal, manteniéndose los valores de 72 horas similares a los de 0 horas. La inclusión de bestatina en todos los diluyentes tuvo un efecto positivo en la conservación del semen, manteniéndose a lo largo del tiempo las características seminales y el grado de lipoperoxidación, independientemente de que el diluyente estuviera suplementado o no

[EN] There is increasing interest in using rabbits for research as a laboratory model as well as for industrial production of meat, wool and fur. Superovulation in animals is used to produce a maximum number of transferable embryos per donor, in order to either support genetic improvement programs, ex situ conservation or to optimize other biotechnologies. Over time, the use of this biotechnology has shown variable outcomes as a consequence of several factors, such as the origin of exogenous hormone, pos- ology and the effect of gonadotropins used simultaneously, the donor and the environment. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (CTP), alone or sup- plemented with LH, versus a FSH standard protocol of five equal doses administered twice daily to su- perovulate rabbit does (20 per group and 29 control females). We determined: 1) the impact of this stimulation on in vitro development and mRNA expression at blastocyst stage and 2) in vivo embryo development and viability rate at birth of transferred embryos. Our outcomes showed that the ovulation rate was similar among the different ovarian stimulation groups, reaching more than fourfold the ovulation rate of a control doe. While rates of embryos developing to the blastocyst stage after 48 h of in vitro culture were similar between groups, the hatched blastocyst rate was higher for superovulated embryos from CTP group. Moreover, no significant differences among mRNA expression of OCT4, SOX2 and NANOG genes were detected. Nevertheless, embryos from ovarian stimulated does with CTP þ LH showed significantly higher implantation rates and survival at birth among the different ovarian stim- ulation groups and similar to those in the control group. In conclusion, the results of this study suggest that a single injection of long acting corifollitropin alfa can be effectively used in rabbits to elicit a more than fourfold increase in ovulation rate compared to control

Storage of rabbit semen for longer than 36 h causes deterioration of semen quality. Therefore, the aim of this study was to evaluate the addition of antioxidants, membrane protective and/or aminopeptidase activity substances to an EDTA supplemented extender on rabbit semen quality preserved at 16ºC during 72 h. Experiment one was carried out to evaluate the effect of the inclusion of two water soluble analogs of vitamin E (Trolox and ¿-tocopherol) and / or dextran on spermatozoa motility, viability, acrosome status and membrane functionality. In a second experiment the inclusion of bestatin in the extenders with Trolox and / or dextran on seminal quality and lipid oxidation was evaluated. The presence of ¿-tocopherol in the extender significantly decreased all seminal quality parameters over time, whereas the presence of Trolox and dextran did not affect them. In the second experiment, no significant differences among the experimental extenders were observed either for the seminal quality parameters or for the lipid oxidation of the seminal plasma at 72 hours of refrigeration. The cooling time significantly affected the viability and integrity of the acrosome (P <0.05), however, no significant differences were observed in the kinetic parameters, membrane functionality and lipid oxidation of the seminal plasma, showing at 72 and 0 hours similar values. The inclusion of bestatin in all extenders had a positive effect on semen conservation, maintaining the semen characteristics and lipid oxidation over time, regardless of whether the extender was supplemented or not with dextran or Trolox. In conclusion, the combination of EDTA and bestatin proved to be a suitable alternative in the refrigerated semen extender since it avoids the antioxidant activity and maintains membrane motility and functionality over the cooling period studied., El semen de conejo puede ser conservado en refrigeración un corto periodo de tiempo antes de que se reduzca o pierda su capacidad fecundante, por lo que no suele utilizarse en inseminación artificial más allá de 36 horas desde su extracción. Por ello el objetivo del presente estudio fue estudiar el efecto de la suplementación de sustancias con actividad protectora de membrana, antioxidante y antiproteasa a un diluyente con EDTA sobre la calidad espermática y la oxidación lipídica del plasma seminal del semen refrigerado a 16ºC durante 72 horas. En un primer experimento se evaluó el efecto de la inclusión de dos análogos hidrosolubles de la vitamina E (Trolox y ¿-tocoferol) y/o el dextrano sobre los parámetros de calidad seminal: motilidad, viabilidad, estado del acrosoma y funcionalidad de la membrana. En un segundo experimento se evaluó la inclusión de la bestatina en los diluyentes con Trolox y/o dextrano sobre la calidad seminal y la oxidación de lípidos. La inclusión de ¿-tocoferol en el diluyente disminuyó significativamente los valores de todos los parámetros de calidad seminal a lo largo del tiempo, mientras que la presencia de Trolox y dextrano no afectó a las mismas. En el segundo experimento el diluyente utilizado no afectó significativamente (P>0,05) a los parámetros de calidad seminal ni a la oxidación lipídica del plasma seminal a las 72 horas de refrigeración. El tiempo de refrigeración afectó significativamente a la viabilidad y a la integridad del acrosoma (P<0,05), sin embargo no se observaron diferencias significativas en los parámetros cinéticos, de funcionalidad de membrana y de oxidación lipídica del plasma seminal, manteniéndose los valores de 72 horas similares a los de 0 horas. La inclusión de bestatina en todos los diluyentes tuvo un efecto positivo en la conservación del semen, manteniéndose a lo largo del tiempo las características seminales y el grado de lipoperoxidación, independientemente de que el diluyente estuviera suplementado o no

[EN] There is increasing interest in using rabbits for research as a laboratory model as well as for industrial production of meat, wool and fur. Superovulation in animals is used to produce a maximum number of transferable embryos per donor, in order to either support genetic improvement programs, ex situ conservation or to optimize other biotechnologies. Over time, the use of this biotechnology has shown variable outcomes as a consequence of several factors, such as the origin of exogenous hormone, pos- ology and the effect of gonadotropins used simultaneously, the donor and the environment. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (CTP), alone or sup- plemented with LH, versus a FSH standard protocol of five equal doses administered twice daily to su- perovulate rabbit does (20 per group and 29 control females). We determined: 1) the impact of this stimulation on in vitro development and mRNA expression at blastocyst stage and 2) in vivo embryo development and viability rate at birth of transferred embryos. Our outcomes showed that the ovulation rate was similar among the different ovarian stimulation groups, reaching more than fourfold the ovulation rate of a control doe. While rates of embryos developing to the blastocyst stage after 48 h of in vitro culture were similar between groups, the hatched blastocyst rate was higher for superovulated embryos from CTP group. Moreover, no significant differences among mRNA expression of OCT4, SOX2 and NANOG genes were detected. Nevertheless, embryos from ovarian stimulated does with CTP þ LH showed significantly higher implantation rates and survival at birth among the different ovarian stim- ulation groups and similar to those in the control group. In conclusion, the results of this study suggest that a single injection of long acting corifollitropin alfa can be effectively used in rabbits to elicit a more than fourfold increase in ovulation rate compared to control

Storage of rabbit semen for longer than 36 h causes deterioration of semen quality. Therefore, the aim of this study was to evaluate the addition of antioxidants, membrane protective and/or aminopeptidase activity substances to an EDTA supplemented extender on rabbit semen quality preserved at 16ºC during 72 h. Experiment one was carried out to evaluate the effect of the inclusion of two water soluble analogs of vitamin E (Trolox and ¿-tocopherol) and / or dextran on spermatozoa motility, viability, acrosome status and membrane functionality. In a second experiment the inclusion of bestatin in the extenders with Trolox and / or dextran on seminal quality and lipid oxidation was evaluated. The presence of ¿-tocopherol in the extender significantly decreased all seminal quality parameters over time, whereas the presence of Trolox and dextran did not affect them. In the second experiment, no significant differences among the experimental extenders were observed either for the seminal quality parameters or for the lipid oxidation of the seminal plasma at 72 hours of refrigeration. The cooling time significantly affected the viability and integrity of the acrosome (P <0.05), however, no significant differences were observed in the kinetic parameters, membrane functionality and lipid oxidation of the seminal plasma, showing at 72 and 0 hours similar values. The inclusion of bestatin in all extenders had a positive effect on semen conservation, maintaining the semen characteristics and lipid oxidation over time, regardless of whether the extender was supplemented or not with dextran or Trolox. In conclusion, the combination of EDTA and bestatin proved to be a suitable alternative in the refrigerated semen extender since it avoids the antioxidant activity and maintains membrane motility and functionality over the cooling period studied., El semen de conejo puede ser conservado en refrigeración un corto periodo de tiempo antes de que se reduzca o pierda su capacidad fecundante, por lo que no suele utilizarse en inseminación artificial más allá de 36 horas desde su extracción. Por ello el objetivo del presente estudio fue estudiar el efecto de la suplementación de sustancias con actividad protectora de membrana, antioxidante y antiproteasa a un diluyente con EDTA sobre la calidad espermática y la oxidación lipídica del plasma seminal del semen refrigerado a 16ºC durante 72 horas. En un primer experimento se evaluó el efecto de la inclusión de dos análogos hidrosolubles de la vitamina E (Trolox y ¿-tocoferol) y/o el dextrano sobre los parámetros de calidad seminal: motilidad, viabilidad, estado del acrosoma y funcionalidad de la membrana. En un segundo experimento se evaluó la inclusión de la bestatina en los diluyentes con Trolox y/o dextrano sobre la calidad seminal y la oxidación de lípidos. La inclusión de ¿-tocoferol en el diluyente disminuyó significativamente los valores de todos los parámetros de calidad seminal a lo largo del tiempo, mientras que la presencia de Trolox y dextrano no afectó a las mismas. En el segundo experimento el diluyente utilizado no afectó significativamente (P>0,05) a los parámetros de calidad seminal ni a la oxidación lipídica del plasma seminal a las 72 horas de refrigeración. El tiempo de refrigeración afectó significativamente a la viabilidad y a la integridad del acrosoma (P<0,05), sin embargo no se observaron diferencias significativas en los parámetros cinéticos, de funcionalidad de membrana y de oxidación lipídica del plasma seminal, manteniéndose los valores de 72 horas similares a los de 0 horas. La inclusión de bestatina en todos los diluyentes tuvo un efecto positivo en la conservación del semen, manteniéndose a lo largo del tiempo las características seminales y el grado de lipoperoxidación, independientemente de que el diluyente estuviera suplementado o no