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Zhou, Xuechang., Thesis (M.Phil.)--Chinese University of Hong Kong, 2007., Includes bibliographical references (leaves 51-53)., s in English and Chinese., p.i, 摘要 --- p.ii, Acknowledgement --- p.iii, Table of contents --- p.iv, Chapter 1 --- Introduction --- p.1, Chapter 1.1 --- Protein crystallization --- p.1, Chapter 1.1.1 --- The principle of protein crystallization --- p.3, Chapter 1.1.2 --- Protein crystallization approaches --- p.4, Chapter 1.1.3 --- Screening strategies and approaches --- p.6, Chapter 1.2 --- Microfluidic systems for protein crystallization --- p.7, Chapter 1.2.1 --- Integrated valve-controlled microfluidic system --- p.8, Chapter 1.2.2 --- Droplet-based microfluidic system --- p.9, Chapter 1.2.3 --- Objective of the research --- p.10, Chapter 2 --- Nanoliter Liquid Dispensing Method --- p.12, Chapter 2.1 --- Introduction --- p.12, Chapter 2.2 --- Experimental --- p.14, Chapter 2.2.1 --- The fabrication of SU-8 master --- p.14, Chapter 2.2.2 --- The fabrication of PDMS microfluidic device --- p.16, Chapter 2.2.3 --- The fabrication of glass and PMMA microwells --- p.17, Chapter 2.2.4 --- The liquid dispensing into microwells --- p.17, Chapter 2.3 --- Results and discussions --- p.20, Chapter 2.3.1 --- The internal vacuum pumping source --- p.20, Chapter 2.3.2 --- The efficiency of the pumping --- p.23, Chapter 2.3.3 --- The removal of PDMS channel patch --- p.26, Chapter 2.4 --- Conclusion --- p.29, Chapter 3 --- The Screening of Protein Crystallization Conditions --- p.30, Chapter 3.1 --- Introduction --- p.30, Chapter 3.2 --- Experimental --- p.31, Chapter 3.2.1 --- The design and fabrication of the screening chip --- p.31, Chapter 3.2.2 --- The screening of protein crystallization conditions --- p.32, Chapter 3.2.3 --- Crystallization and X-ray diffraction of an unknown protein --- p.33, Chapter 3.3 --- Results and discussions --- p.33, Chapter 3.3.1 --- Sparse matrix screening strategy in microwell arrays --- p.33, Chapter 3.3.2 --- The results of the sparse matrix screening --- p.39, Chapter 3.3.3 --- Crystal extraction and X-ray diffraction --- p.40, Chapter 3.4 --- Conclusion --- p.41, Chapter 4 --- Conclusion --- p.43, Chapter 4.1 --- Summary --- p.43, Chapter 4.2 --- Discussions and future directions --- p.44, Appendix Information --- p.47, References --- p.51, http://library.cuhk.edu.hk/record=b5893176, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)