A microwell-based microfluidic platform for high-throughput screening of protein crystallization conditions.

Zhou, Xuechang.
Thesis (M.Phil.)--Chinese University of Hong Kong, 2007.
Includes bibliographical references (leaves 51-53).
s in English and Chinese.
p.i
摘要 --- p.ii
Acknowledgement --- p.iii
Table of contents --- p.iv
Chapter 1 --- Introduction --- p.1
Chapter 1.1 --- Protein crystallization --- p.1
Chapter 1.1.1 --- The principle of protein crystallization --- p.3
Chapter 1.1.2 --- Protein crystallization approaches --- p.4
Chapter 1.1.3 --- Screening strategies and approaches --- p.6
Chapter 1.2 --- Microfluidic systems for protein crystallization --- p.7
Chapter 1.2.1 --- Integrated valve-controlled microfluidic system --- p.8
Chapter 1.2.2 --- Droplet-based microfluidic system --- p.9
Chapter 1.2.3 --- Objective of the research --- p.10
Chapter 2 --- Nanoliter Liquid Dispensing Method --- p.12
Chapter 2.1 --- Introduction --- p.12
Chapter 2.2 --- Experimental --- p.14
Chapter 2.2.1 --- The fabrication of SU-8 master --- p.14
Chapter 2.2.2 --- The fabrication of PDMS microfluidic device --- p.16
Chapter 2.2.3 --- The fabrication of glass and PMMA microwells --- p.17
Chapter 2.2.4 --- The liquid dispensing into microwells --- p.17
Chapter 2.3 --- Results and discussions --- p.20
Chapter 2.3.1 --- The internal vacuum pumping source --- p.20
Chapter 2.3.2 --- The efficiency of the pumping --- p.23
Chapter 2.3.3 --- The removal of PDMS channel patch --- p.26
Chapter 2.4 --- Conclusion --- p.29
Chapter 3 --- The Screening of Protein Crystallization Conditions --- p.30
Chapter 3.1 --- Introduction --- p.30
Chapter 3.2 --- Experimental --- p.31
Chapter 3.2.1 --- The design and fabrication of the screening chip --- p.31
Chapter 3.2.2 --- The screening of protein crystallization conditions --- p.32
Chapter 3.2.3 --- Crystallization and X-ray diffraction of an unknown protein --- p.33
Chapter 3.3 --- Results and discussions --- p.33
Chapter 3.3.1 --- Sparse matrix screening strategy in microwell arrays --- p.33
Chapter 3.3.2 --- The results of the sparse matrix screening --- p.39
Chapter 3.3.3 --- Crystal extraction and X-ray diffraction --- p.40
Chapter 3.4 --- Conclusion --- p.41
Chapter 4 --- Conclusion --- p.43
Chapter 4.1 --- Summary --- p.43
Chapter 4.2 --- Discussions and future directions --- p.44
Appendix Information --- p.47
References --- p.51
http://library.cuhk.edu.hk/record=b5893176
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