40 results on '"Yadava N"'
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2. Role of pinch in Argon impurity transport in ohmic discharges of Aditya-U Tokamak.
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Shah K, Ghosh J, Patel S, Chowdhuri MB, Jadeja KA, Shukla G, Macwan T, Kumar A, Dolui S, Singh K, Tanna RL, Patel KM, Dey R, Manchanda R, Ramaiya N, Kumar R, Aich S, Yadava N, Purohit S, Gupta MK, Nagora UC, Pathak SK, Atrey PK, and Mayya KBK
- Abstract
We present experimental results of the trace argon impurity puffing in the ohmic plasmas of Aditya-U tokamak performed to study the argon transport behaviour. Argon line emissions in visible and Vacuum Ultra Violet (VUV) spectral ranges arising from the plasma edge and core respectively are measured simultaneously. During the experiments, space resolved brightness profile of Ar
1+ line emissions at 472.69 nm (3p4 4s2 P3/2 -3p4 4p2 D3/2 ), 473.59 nm (3p4 4s4 P5/2 -3p4 4p4 P3/2 ), 476.49 nm (3p4 4s2 P1/2 -3p4 4p2 P3/2 ), 480.60 nm (3p4 4s4 P5/2 -3p4 4p4 P5/2 ) are recorded using a high resolution visible spectrometer. Also, a VUV spectrometer has been used to simultaneously observe Ar13+ line emission at 18.79 nm (2s2 2p2 P3/2 -2s2p2 2 P3/2 ) and Ar14+ line emission at 22.11 nm (2s2 1 S0 -2s2p1 P1 ). The diffusivity and convective velocity of Ar are obtained by comparing the measured radial emissivity profile of Ar1+ emission and the line intensity ratio of Ar13+ and Ar14+ ions, with those simulated using the impurity transport code, STRAHL. Argon diffusivities ~ 12 m2 /s and ~ 0.3 m2 /s have been observed in the edge (ρ > 0.85) and core region of the Aditya-U, respectively. The diffusivity values both in the edge and core region are found to be higher than the neo-classical values suggesting that the argon impurity transport is mainly anomalous in the Aditya-U tokamak. Also, an inward pinch of ~ 10 m/s mainly driven by Ware pinch is required to match the measured and simulated data. The measured peaked profile of Ar density suggests impurity accumulation in these discharges., (© 2023. The Author(s).)- Published
- 2023
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3. Initial results from near-infrared spectroscopy on ADITYA-U tokamak.
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Ramaiya N, Manchanda R, Chowdhuri MB, Yadava N, Dey R, Kumar A, Shah K, Patel S, Jadeja KA, Patel KM, Kumar R, Aich S, Pathak SK, Tanna RL, and Ghosh J
- Abstract
Spectroscopy in vacuum ultraviolet (VUV) and visible ranges plays an important role in the investigation and diagnosis of tokamak plasmas. However, under harsh environmental conditions of fusion grade devices, such as ITER, VUV-visible systems encounter many issues due to the degradation of optical components used in such systems. Here, near-infrared (NIR) spectroscopy has become an effective tool in understanding the edge plasma dynamics. Considering its importance, a NIR spectroscopic diagnostic has been developed and installed on the ADITYA-U tokamak. The system consists of a 0.5 m spectrometer having three gratings with different groove densities, and it is coupled with a linear InGaAs photodiode array. Radiation from the ADITYA-U edge plasma has been collected using a collimating lens and optical fiber combination and transported to the spectrometer. The spectrum in the NIR range from the ADITYA-U plasma has been recorded using this system, in which Pa
β and Paγ along with many spectral lines from neutral and singly ionized impurities have been observed. The influxes of H and C have been estimated from measurements. The H influx value is found to be 2.8 × 1016 and 1.9 × 1016 particles cm-2 s-1 from neutral hydrogen lines Hα and Paβ , respectively, and the C influx value is found to be 3.5 × 1015 and 2.9 × 1015 particles cm-2 s-1 from the neutral carbon and singly ionized carbon, respectively. A good agreement is seen between these results and the results obtained by using a routine photomultiplier tube based diagnostic.- Published
- 2022
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4. Pyruvate-Driven Oxidative Phosphorylation is Downregulated in Sepsis-Induced Cardiomyopathy: A Study of Mitochondrial Proteome.
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Shimada BK, Boyman L, Huang W, Zhu J, Yang Y, Chen F, Kane MA, Yadava N, Zou L, Lederer WJ, Polster BM, and Chao W
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- Animals, Male, Mice, Mitochondria metabolism, Mitochondrial Proteins, Myocardium metabolism, Oxidative Phosphorylation, Proteome metabolism, Pyruvate Dehydrogenase Complex metabolism, Pyruvic Acid metabolism, Cardiomyopathies etiology, Cardiomyopathies metabolism, Sepsis complications, Sepsis metabolism
- Abstract
Background: Sepsis-induced cardiomyopathy (SIC) is a major contributing factor for morbidity and mortality in sepsis. Accumulative evidence has suggested that cardiac mitochondrial oxidative phosphorylation is attenuated in sepsis, but the underlying molecular mechanisms remain incompletely understood., Methods: Adult male mice of 9 to 12 weeks old were subjected to sham or cecal ligation and puncture procedure. Echocardiography in vivo and Langendorff-perfused hearts were used to assess cardiac function 24 h after the procedures. Unbiased proteomics analysis was performed to profile mitochondrial proteins in the hearts of both sham and SIC mice. Seahorse respirator technology was used to evaluate oxygen consumption in purified mitochondria., Results: Of the 665 mitochondrial proteins identified in the proteomics assay, 35 were altered in septic mice. The mitochondrial remodeling involved various energy metabolism pathways including subunits of the electron transport chain, fatty acid catabolism, and carbohydrate oxidative metabolism. We also identified a significant increase of pyruvate dehydrogenase (PDH) kinase 4 (PDK4) and inhibition of PDH activity in septic hearts. Furthermore, compared to sham mice, mitochondrial oxygen consumption of septic mice was significantly reduced when pyruvate was provided as a substrate. However, it was unchanged when PDH was bypassed by directly supplying the Complex I substrate NADH, or by using the Complex II substrate succinate, or using Complex IV substrate, or by providing the beta-oxidation substrate palmitoylcarnitine, neither of which require PDH for mitochondrial oxygen consumption., Conclusions: These data demonstrate a broad mitochondrial protein remodeling, PDH inactivation and impaired pyruvate-fueled oxidative phosphorylation during SIC, and provide a molecular framework for further exploration., Competing Interests: The authors report no conflicts of interests., (Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the Shock Society.)
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- 2022
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5. Observations of visible argon line emissions and its spatial profile from Aditya-U tokamak plasma.
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Shah K, Ghosh J, Shukla G, Chowdhuri MB, Manchanda R, Yadava N, Ramaiya N, Jadeja KA, Patel KM, Tanna RL, and Mayya KBK
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The spectroscopic studies of medium and high Z impurities have been the subject of interest in fusion research due to their role in mitigating plasma disruption and reducing heat load on the plasma facing components. Line emissions from these impurities provide the rotation velocity and ion temperature measurements along with the understanding of the overall impurity behavior in plasma. In the Aditya-U tokamak, the spatially resolved Ar II line emissions have been observed using a high resolution multi-track spectroscopic diagnostic consisting of a 1 m Czerny-Turner spectrometer coupled with a charge coupled device (CCD) detector using seven lines of sight viewing plasma tangentially along the toroidal direction. The spatially resolved Ar II lines at 458.96 nm have been observed. The singly ionized Ar emission peaks at the radial location of ρ = 0.8 of the plasma having a minor radius of 25 cm. Moreover, a 0.5 m UV-visible spectrometer coupled with a CCD detector and having a line of sight passing through the plasma midplane from the radial port was used to record visible Ar survey spectra within the 670-810 nm wavelength range, and all these lines have been identified for further analysis.
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- 2021
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6. Mitochondrial Stress Response and Cancer.
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O'Malley J, Kumar R, Inigo J, Yadava N, and Chandra D
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- Activating Transcription Factors metabolism, Antineoplastic Agents therapeutic use, Cell Survival drug effects, Chaperonin 60 metabolism, Disease Progression, Drug Resistance, Neoplasm drug effects, Endoplasmic Reticulum drug effects, Endoplasmic Reticulum pathology, Endoplasmic Reticulum Stress drug effects, Humans, Mitochondria drug effects, Mitochondrial Proteins metabolism, Neoplasms drug therapy, Oxidative Stress drug effects, Reactive Oxygen Species metabolism, Signal Transduction drug effects, Antineoplastic Agents pharmacology, Mitochondria pathology, Neoplasms pathology, Unfolded Protein Response drug effects
- Abstract
Cancer cells survive and adapt to many types of stress including hypoxia, nutrient deprivation, metabolic, and oxidative stress. These stresses are sensed by diverse cellular signaling processes, leading to either degradation of mitochondria or alleviation of mitochondrial stress. This review discusses signaling during sensing and mitigation of stress involving mitochondrial communication with the endoplasmic reticulum, and how retrograde signaling upregulates the mitochondrial stress response to maintain mitochondrial integrity. The importance of the mitochondrial unfolded protein response, an emerging pathway that alleviates cellular stress, will be elaborated with respect to cancer. Detailed understanding of cellular pathways will establish mitochondrial stress response as a key mechanism for cancer cell survival leading to cancer progression and resistance, and provide a potential therapeutic target in cancer., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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7. Mapping mitochondrial respiratory chain deficiencies by respirometry: Beyond the Mito Stress Test.
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Jaber SM, Yadava N, and Polster BM
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- Animals, Cell Line, Cell Respiration, Humans, Neurons, Rats, Biological Assay, Energy Metabolism, In Vitro Techniques, Mitochondrial Diseases
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Cell-based respirometers, such as the Seahorse Extracellular Flux Analyzer, are valuable tools to assess the functionality of mitochondria within adherent neurons, as well as other cell types. The Mito Stress Test is the most frequently employed protocol of drug additions to evaluate mitochondrial bioenergetic function. Sequential exposure of cells to an ATP synthase inhibitor such as oligomycin and an uncoupler such as FCCP cause changes in oxygen consumption rate that allow estimation of the cellular efficiency and capacity for mitochondrial ATP synthesis. While a useful first step in assessing whether an experimental treatment or genetic manipulation affects mitochondrial energetics, the Mito Stress Test does not identify specific sites of altered respiratory chain function. This article discusses limitations of the Mito Stress Test, proposes a refined protocol for comparing cell populations that requires independent drug titrations at multiple cell densities, and describes a stepwise series of respirometry-based assays that "map" locations of electron transport deficiency. These include strategies to test for cytochrome c release, to probe the functionality of specific electron transport chain complexes within intact or permeabilized cells, and to measure NADH oxidation by the linked activity of Complexes I, III, and IV. To illustrate utility, we show that although UK5099 and ABT-737 each decrease the spare respiratory capacity of cortical neurons, the stepwise assays reveal different underlying mechanisms consistent with their established drug targets: deficient Complex I substrate supply induced by the mitochondrial pyruvate carrier inhibitor UK5099 and cytochrome c release induced by the anti-apoptotic BCL-2 family protein inhibitor ABT-737., Competing Interests: Declaration of Competing Interest N.Y. receives royalties from Agilent Technologies for U.S. Patent US9915647B2. Agilent Technologies had no role in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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8. Anionic Polymers Promote Mitochondrial Targeting of Delocalized Lipophilic Cations.
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Jiang Z, Liu H, He H, Yadava N, Chambers JJ, and Thayumanavan S
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- HeLa Cells, Humans, Kinetics, Membrane Potential, Mitochondrial, Drug Carriers chemistry, Drug Carriers metabolism, Hydrophobic and Hydrophilic Interactions, Mitochondria metabolism, Polymers chemistry, Polymers metabolism
- Abstract
Mitochondria are therapeutic targets in many diseases including cancer, metabolic disorders, and neurodegenerative diseases. Therefore, strategies to deliver therapeutics of interest to mitochondria are important for therapeutic development. As delocalized lipophilic cations (DLCs) preferentially accumulate in mitochondria, DLC-conjugation has been utilized to facilitate therapeutic delivery systems with mitochondrial targeting capability. Here we report that upon DLC-conjugation, anionic polymers exhibit significantly improved mitochondrial targeting when compared to cationic polymers and charge-neutral polymers. Considering that the cell membrane generally bears a net negative charge, the observed phenomenon is unexpected. Notably, the DLC-conjugated anionic polymers circumvent endosomal entrapment. The rapid mitochondrial accumulation of DLC-conjugated anionic polymers is likely a membrane-potential-driven process, along with the involvement of the mitochondrial pyruvate carrier. Moreover, the structural variations on the side chain of DLC-conjugated anionic polymers do not compromise the overall mitochondrial targeting capability, widely extending the applicability of anionic macromolecules in therapeutic delivery systems.
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- 2020
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9. Rotenone Treatment Reveals a Role for Electron Transport Complex I in the Subcellular Localization of Key Transcriptional Regulators During T Helper Cell Differentiation.
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Ozay EI, Sherman HL, Mello V, Trombley G, Lerman A, Tew GN, Yadava N, and Minter LM
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- Animals, Biomarkers, Gene Expression Regulation drug effects, Immunophenotyping, Intracellular Space metabolism, Lymphocyte Activation drug effects, Lymphocyte Activation genetics, Lymphocyte Activation immunology, Mice, Mitochondria drug effects, Mitochondria immunology, Mitochondria metabolism, Nuclear Receptor Subfamily 1, Group F, Member 3 metabolism, Protein Transport, T-Lymphocyte Subsets cytology, T-Lymphocyte Subsets drug effects, T-Lymphocyte Subsets immunology, T-Lymphocyte Subsets metabolism, T-Lymphocytes, Helper-Inducer cytology, Transcription Factors genetics, Cell Differentiation drug effects, Cell Differentiation immunology, Electron Transport Complex I metabolism, Rotenone pharmacology, T-Lymphocytes, Helper-Inducer drug effects, T-Lymphocytes, Helper-Inducer physiology, Transcription Factors metabolism
- Abstract
Recent advances in our understanding of tumor cell mitochondrial metabolism suggest it may be an attractive therapeutic target. Mitochondria are central hubs of metabolism that provide energy during the differentiation and maintenance of immune cell phenotypes. Mitochondrial membranes harbor several enzyme complexes that are involved in the process of oxidative phosphorylation, which takes place during energy production. Data suggest that, among these enzyme complexes, deficiencies in electron transport complex I may differentially affect immune responses and may contribute to the pathophysiology of several immunological conditions. Once activated by T cell receptor signaling, along with co-stimulation through CD28, CD4 T cells utilize mitochondrial energy to differentiate into distinct T helper (Th) subsets. T cell signaling activates Notch1, which is cleaved from the plasma membrane to generate its intracellular form (N1ICD). In the presence of specific cytokines, Notch1 regulates gene transcription related to cell fate to modulate CD4 Th type 1, Th2, Th17, and induced regulatory T cell (iTreg) differentiation. The process of differentiating into any of these subsets requires metabolic energy, provided by the mitochondria. We hypothesized that the requirement for mitochondrial metabolism varies between different Th subsets and may intersect with Notch1 signaling. We used the organic pesticide rotenone, a well-described complex I inhibitor, to assess how compromised mitochondrial integrity impacts CD4 T cell differentiation into Th1, Th2, Th17, and iTreg cells. We also investigated how Notch1 localization and downstream transcriptional capabilities regulation may be altered in each subset following rotenone treatment. Our data suggest that mitochondrial integrity impacts each of these Th subsets differently, through its influence on Notch1 subcellular localization. Our work further supports the notion that altered immune responses can result from complex I inhibition. Therefore, understanding how mitochondrial inhibitors affect immune responses may help to inform therapeutic approaches to cancer treatment.
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- 2018
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10. Evaluation of Rhodiola crenulata on growth and metabolism of NB-1691, an MYCN-amplified neuroblastoma cell line.
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Wong KE, Mora MC, Sultana N, Moriarty KP, Arenas RB, Yadava N, Schneider SS, and Tirabassi MV
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- Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Cytotoxins pharmacology, Humans, N-Myc Proto-Oncogene Protein genetics, Neuroblastoma genetics, Rhodiola, Antineoplastic Agents pharmacology, Cell Respiration drug effects, Neuroblastoma metabolism, Phytotherapy methods, Plant Extracts pharmacology
- Abstract
Outcomes of children with high grade neuroblastoma remain poor despite multi-agent chemotherapy regimens. Rhodiola crenulata extracts display anti-neoplastic properties against several cancers including breast cancer, melanoma, and glioblastoma. In this study, we evaluated the anti-neoplastic potential of Rhodiola crenulata extracts on human neuroblastoma cells. Through this work, cell viability and proliferation were evaluated following treatments with ethanol (vehicle control) or Rhodiola crenulata extract in neuroblastoma, NB-1691 or SK-N-AS cells, in vitro. HIF-1 transcriptional activity was evaluated using a dual luciferase assay. Quantitative real-time polymerase chain reaction was utilized to assess the expression of HIF-1 targets. Selected metabolic intermediates were evaluated for their ability to rescue cells from Rhodiola crenulata extract-induced death. Lactate dehydrogenase, pyruvate kinase, and pyruvate dehydrogenase activities and NAD
+ /NADH levels were assayed in vehicle and Rhodiola crenulata extract-treated cells. The effects of Rhodiola crenulata extracts on metabolism were assessed by respirometry and metabolic phenotyping/fingerprinting. Our results revealed striking cytotoxic effects upon Rhodiola crenulata extract treatment, especially prominent in NB-1691 cells. As a greater response was observed in NB-1691 cells therefore it was used for remaining experiments. Upon Rhodiola crenulata extract treatment, HIF-1 transcriptional activity was increased. This increase in activity correlated with changes in HIF-1 targets involved in cellular metabolism. Serendipitously, we observed that addition of pyruvate protected against the cytotoxic effects of Rhodiola crenulata extracts. Therefore, we focused on the metabolic effects of Rhodiola crenulata extracts on NB-1691 cells. We observed that while the activities of pyruvate kinase and pyruvate dehydrogenase activities were increased, the activity of lactate dehydrogenase activity was decreased upon Rhodiola crenulata extract treatment. We also noted a decline in the total NAD pool following Rhodiola crenulata extract treatment. This correlated with decreased cellular respiration and suppressed utilization of carbon substrates. Through this work, we observed significant cytotoxic effects of Rhodiola crenulata extract treatment upon treatment on NB-1691 cells, a human neuroblastoma cell line with MYCN amplification. Our studies suggest that these cytotoxic effects could be secondary to metabolic effect induced by treatment with Rhodiola crenulata extract.- Published
- 2018
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11. Individual-specific variation in the respiratory activities of HMECs and their bioenergetic response to IGF1 and TNFα.
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Schneider SS, Henchey EM, Sultana N, Morin SM, Jerry DJ, Makari-Judson G, Crisi GM, Arenas RB, Johnson M, Mason HS, and Yadava N
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- Adult, Aged, Cell Respiration drug effects, Epithelial Cells metabolism, Female, Humans, Mammary Glands, Human metabolism, Metabolomics methods, Middle Aged, Oxidation-Reduction, Phenotype, Pyruvic Acid metabolism, Time Factors, Tumor Cells, Cultured, Young Adult, Breast Neoplasms metabolism, Energy Metabolism drug effects, Epithelial Cells drug effects, Insulin-Like Growth Factor I pharmacology, Mammary Glands, Human drug effects, Tumor Necrosis Factor-alpha pharmacology
- Abstract
Metabolic reprograming is a hallmark of cancer cells. However, the roles of pre-existing differences in normal cells metabolism toward cancer risk is not known. In order to assess pre-existing variations in normal cell metabolism, we have quantified the inter-individual variation in oxidative metabolism of normal primary human mammary epithelial cells (HMECs). We then assessed their response to selected cytokines such as insulin growth factor 1 (IGF1) and tumor necrosis factor alpha (TNFα), which are associated with breast cancer risk. Specifically, we compared the oxidative metabolism of HMECs obtained from women with breast cancer and without cancer. Our data show considerable inter-individual variation in respiratory activities of HMECs from different women. A bioenergetic parameter called pyruvate-stimulated respiration (PySR) was identified as a key distinguishing feature of HMECs from women with breast cancer and without cancer. Samples showing PySR over 20% of basal respiration rate were considered PySR
+ve and the rest as PySR-ve . By this criterion, HMECs from tumor-affected breasts (AB) and non-tumor affected breasts (NAB) of cancer patients were mostly PySR-ve (88% and 89%, respectively), while HMECs from non-cancer patients were mostly PySR+ve (57%). This suggests that PySR-ve/+ve phenotypes are individual-specific and are not caused by field effects due to the presence of tumor. The effects of IGF1 and TNFα treatments on HMECs revealed that both suppressed respiration and extracellular acidification. In addition, IGF1 altered PySR-ve/+ve phenotypes. These results reveal individual-specific differences in pyruvate metabolism of normal breast epithelial cells and its association with breast cancer risk., (© 2017 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc.)- Published
- 2017
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12. An X-chromosome linked mouse model (Ndufa1 S55A ) for systemic partial Complex I deficiency for studying predisposition to neurodegeneration and other diseases.
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Kim C, Potluri P, Khalil A, Gaut D, McManus M, Compton S, Wallace DC, and Yadava N
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- Animals, Body Temperature physiology, Exhalation physiology, Female, Genetic Predisposition to Disease genetics, Male, Mice, Mice, 129 Strain, Mice, Transgenic, Pregnancy, Electron Transport Complex I deficiency, Electron Transport Complex I genetics, Genes, X-Linked genetics, Membrane Proteins genetics, Neurodegenerative Diseases genetics, Neurodegenerative Diseases metabolism
- Abstract
The respiratory chain Complex I deficiencies are the most common cause of mitochondrial diseases. Complex I biogenesis is controlled by 58 genes and at least 47 of these cause mitochondrial disease in humans. Two of these are X-chromosome linked nuclear (nDNA) genes (NDUFA1 and NDUFB11), and 7 are mitochondrial (mtDNA, MT-ND1-6, -4L) genes, which may be responsible for sex-dependent variation in the presentation of mitochondrial diseases. In this study, we describe an X-chromosome linked mouse model (Ndufa1
S55A ) for systemic partial Complex I deficiency. By homologous recombination, a point mutation T > G within 55th codon of the Ndufa1 gene was introduced. The resulting allele Ndufa1S55A introduced systemic serine-55-alanine (S55A) mutation within the MWFE protein, which is essential for Complex I assembly and stability. The S55A mutation caused systemic partial Complex I deficiency of ∼50% in both sexes. The mutant males (Ndufa1S55A/Y ) displayed reduced respiratory exchange ratio (RER) and produced less body heat. They were also hypoactive and ate less. They showed age-dependent Purkinje neurons degeneration. Metabolic profiling of brain, liver and serum from males showed reduced heme levels in mutants, which correlated with altered expressions of Fech and Hmox1 mRNAs in tissues. This is the first genuine X-chromosome linked mouse model for systemic partial Complex I deficiency, which shows age-dependent neurodegeneration. The effect of Complex I deficiency on survival patterns of males vs. females was different. We believe this model will be very useful for studying sex-dependent predisposition to both spontaneous and stress-induced neurodegeneration, cancer, diabetes and other diseases., (Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.)- Published
- 2017
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13. The Putative Drp1 Inhibitor mdivi-1 Is a Reversible Mitochondrial Complex I Inhibitor that Modulates Reactive Oxygen Species.
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Bordt EA, Clerc P, Roelofs BA, Saladino AJ, Tretter L, Adam-Vizi V, Cherok E, Khalil A, Yadava N, Ge SX, Francis TC, Kennedy NW, Picton LK, Kumar T, Uppuluri S, Miller AM, Itoh K, Karbowski M, Sesaki H, Hill RB, and Polster BM
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- Animals, COS Cells, Cell Respiration drug effects, Chlorocebus aethiops, Dynamins metabolism, Electron Transport Complex I metabolism, Fibroblasts metabolism, Fibroblasts ultrastructure, GTP Phosphohydrolases metabolism, Humans, Mice, Mice, Knockout, Microtubule-Associated Proteins metabolism, Mitochondria drug effects, Mitochondrial Proteins metabolism, NAD metabolism, Neurons metabolism, Oxidation-Reduction drug effects, Oxygen Consumption drug effects, Rats, Sprague-Dawley, Saccharomyces cerevisiae drug effects, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism, Dynamins antagonists & inhibitors, Electron Transport Complex I antagonists & inhibitors, GTP Phosphohydrolases antagonists & inhibitors, Microtubule-Associated Proteins antagonists & inhibitors, Mitochondria metabolism, Mitochondrial Proteins antagonists & inhibitors, Quinazolinones pharmacology, Reactive Oxygen Species metabolism
- Abstract
Mitochondrial fission mediated by the GTPase dynamin-related protein 1 (Drp1) is an attractive drug target in numerous maladies that range from heart disease to neurodegenerative disorders. The compound mdivi-1 is widely reported to inhibit Drp1-dependent fission, elongate mitochondria, and mitigate brain injury. Here, we show that mdivi-1 reversibly inhibits mitochondrial complex I-dependent O
2 consumption and reverse electron transfer-mediated reactive oxygen species (ROS) production at concentrations (e.g., 50 μM) used to target mitochondrial fission. Respiratory inhibition is rescued by bypassing complex I using yeast NADH dehydrogenase Ndi1. Unexpectedly, respiratory impairment by mdivi-1 occurs without mitochondrial elongation, is not mimicked by Drp1 deletion, and is observed in Drp1-deficient fibroblasts. In addition, mdivi-1 poorly inhibits recombinant Drp1 GTPase activity (Ki > 1.2 mM). Overall, these results suggest that mdivi-1 is not a specific Drp1 inhibitor. The ability of mdivi-1 to reversibly inhibit complex I and modify mitochondrial ROS production may contribute to effects observed in disease models., (Copyright © 2017 Elsevier Inc. All rights reserved.)- Published
- 2017
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14. Mechanism of neem limonoids-induced cell death in cancer: Role of oxidative phosphorylation.
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Yadav N, Kumar S, Kumar R, Srivastava P, Sun L, Rapali P, Marlowe T, Schneider A, Inigo JR, O'Malley J, Londonkar R, Gogada R, Chaudhary AK, Yadava N, and Chandra D
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- Caspases metabolism, Cyclin-Dependent Kinase Inhibitor p21 physiology, DNA, Mitochondrial analysis, Dynamins, Electron Transport Complex I physiology, GTP Phosphohydrolases analysis, HCT116 Cells, Humans, Microtubule-Associated Proteins analysis, Mitochondrial Membrane Transport Proteins physiology, Mitochondrial Permeability Transition Pore, Mitochondrial Proteins analysis, Neoplasms drug therapy, Tumor Suppressor Protein p53 physiology, Apoptosis drug effects, Azadirachta chemistry, Limonins pharmacology, Neoplasms pathology, Oxidative Phosphorylation
- Abstract
We have previously reported that neem limonoids (neem) induce multiple cancer cell death pathways. Here we dissect the underlying mechanisms of neem-induced apoptotic cell death in cancer. We observed that neem-induced caspase activation does not require Bax/Bak channel-mediated mitochondrial outer membrane permeabilization, permeability transition pore, and mitochondrial fragmentation. Neem enhanced mitochondrial DNA and mitochondrial biomass. While oxidative phosphorylation (OXPHOS) Complex-I activity was decreased, the activities of other OXPHOS complexes including Complex-II and -IV were unaltered. Increased reactive oxygen species (ROS) levels were associated with an increase in mitochondrial biomass and apoptosis upon neem exposure. Complex-I deficiency due to the loss of Ndufa1-encoded MWFE protein inhibited neem-induced caspase activation and apoptosis, but cell death induction was enhanced. Complex II-deficiency due to the loss of succinate dehydrogenase complex subunit C (SDHC) robustly decreased caspase activation, apoptosis, and cell death. Additionally, the ablation of Complexes-I, -III, -IV, and -V together did not inhibit caspase activation. Together, we demonstrate that neem limonoids target OXPHOS system to induce cancer cell death, which does not require upregulation or activation of proapoptotic Bcl-2 family proteins., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2016
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15. Genetic modification of human mesenchymal stem cells helps to reduce adiposity and improve glucose tolerance in an obese diabetic mouse model.
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Sen S, Domingues CC, Rouphael C, Chou C, Kim C, and Yadava N
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- Animals, Body Composition, Cells, Cultured, Diabetes Mellitus, Experimental genetics, Diabetes Mellitus, Experimental metabolism, Electron Transport Complex I metabolism, Glucose Tolerance Test, Humans, Hyperglycemia genetics, Hyperglycemia metabolism, Mice, Obesity genetics, Obesity metabolism, Oxidative Stress, Oxygen Consumption, Superoxide Dismutase genetics, Adiposity genetics, Diabetes Mellitus, Experimental therapy, Glucose metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Obesity therapy
- Abstract
Introduction: Human mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into fat, muscle, bone and cartilage cells. Exposure of subcutaneous abdominal adipose tissue derived AD-MSCs to high glucose (HG) leads to superoxide accumulation and up-regulation of inflammatory molecules. Our aim was to inquire how HG exposure affects MSCs differentiation and whether the mechanism is reversible., Methods: We exposed human adipose tissue derived MSCs to HG (25 mM) and compared it to normal glucose (NG, 5.5 mM) exposed cells at 7, 10 and 14 days. We examined mitochondrial superoxide accumulation (Mitosox-Red), cellular oxygen consumption rate (OCR, Seahorse) and gene expression., Results: HG increased reactive superoxide (ROS) accumulation noted by day 7 both in cytosol and mitochondria. The OCR between the NG and HG exposed groups however did not change until 10 days at which point OCR of HG exposed cells were reduced significantly. We noted that HG exposure upregulated mRNA expression of adipogenic (PPARG, FABP-4, CREBP alpha and beta), inflammatory (IL-6 and TNF alpha) and antioxidant (SOD2 and Catalase) genes. Next, we used AdSOD2 to upregulate SOD2 prior to HG exposure and thereby noted reduction in superoxide generation. SOD2 upregulation helped reduce mRNA over-expression of PPARG, FABP-4, IL-6 and TNFα. In a series of separate experiments, we delivered the eGFP and SOD2 upregulated MSCs (5 days post ex-vivo transduction) and saline intra-peritoneally (IP) to obese diabetic (db/db) mice. We confirmed homing-in of eGFP labeled MSCs, delivered IP, to different inflamed fat pockets, particularly omental fat. Mice receiving SOD2-MSCs showed progressive reduction in body weight and improved glucose tolerance (GTT) at 4 weeks, post MSCs transplantation compared to the GFP-MSC group (control)., Conclusions: High glucose evokes superoxide generation, OCR reduction and adipogenic differentiation. Mitochondrial superoxide dismutase upregulation quenches excess superoxide and reduces adipocyte inflammation. Delivery of superoxide dismutase (SOD2) using MSCs as a gene delivery vehicle reduces inflammation and improves glucose tolerance in vivo. Suppression of superoxide production and adipocyte inflammation using mitochondrial superoxide dismutase may be a novel and safe therapeutic tool to combat hyperglycemia mediated effects.
- Published
- 2015
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16. Oxidative phosphorylation-dependent regulation of cancer cell apoptosis in response to anticancer agents.
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Yadav N, Kumar S, Marlowe T, Chaudhary AK, Kumar R, Wang J, O'Malley J, Boland PM, Jayanthi S, Kumar TK, Yadava N, and Chandra D
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- Apoptosis drug effects, Humans, Neoplasms genetics, Neoplasms metabolism, Neoplasms pathology, Oxidative Phosphorylation, Signal Transduction, Up-Regulation, Antineoplastic Agents pharmacology, Neoplasms drug therapy
- Abstract
Cancer cells tend to develop resistance to various types of anticancer agents, whether they adopt similar or distinct mechanisms to evade cell death in response to a broad spectrum of cancer therapeutics is not fully defined. Current study concludes that DNA-damaging agents (etoposide and doxorubicin), ER stressor (thapsigargin), and histone deacetylase inhibitor (apicidin) target oxidative phosphorylation (OXPHOS) for apoptosis induction, whereas other anticancer agents including staurosporine, taxol, and sorafenib induce apoptosis in an OXPHOS-independent manner. DNA-damaging agents promoted mitochondrial biogenesis accompanied by increased accumulation of cellular and mitochondrial ROS, mitochondrial protein-folding machinery, and mitochondrial unfolded protein response. Induction of mitochondrial biogenesis occurred in a caspase activation-independent mechanism but was reduced by autophagy inhibition and p53-deficiency. Abrogation of complex-I blocked DNA-damage-induced caspase activation and apoptosis, whereas inhibition of complex-II or a combined deficiency of OXPHOS complexes I, III, IV, and V due to impaired mitochondrial protein synthesis did not modulate caspase activity. Mechanistic analysis revealed that inhibition of caspase activation in response to anticancer agents associates with decreased release of mitochondrial cytochrome c in complex-I-deficient cells compared with wild type (WT) cells. Gross OXPHOS deficiencies promoted increased release of apoptosis-inducing factor from mitochondria compared with WT or complex-I-deficient cells, suggesting that cells harboring defective OXPHOS trigger caspase-dependent as well as caspase-independent apoptosis in response to anticancer agents. Interestingly, DNA-damaging agent doxorubicin showed strong binding to mitochondria, which was disrupted by complex-I-deficiency but not by complex-II-deficiency. Thapsigargin-induced caspase activation was reduced upon abrogation of complex-I or gross OXPHOS deficiency whereas a reverse trend was observed with apicidin. Together, these finding provide a new strategy for differential mitochondrial targeting in cancer therapy.
- Published
- 2015
- Full Text
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17. The 2-oxoacid dehydrogenase complexes in mitochondria can produce superoxide/hydrogen peroxide at much higher rates than complex I.
- Author
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Quinlan CL, Goncalves RL, Hey-Mogensen M, Yadava N, Bunik VI, and Brand MD
- Subjects
- Animals, Female, Mitochondria, Muscle enzymology, Muscle, Skeletal enzymology, Muscle, Skeletal metabolism, NAD metabolism, Oxidation-Reduction, Pyruvate Dehydrogenase Complex metabolism, Rats, Rats, Wistar, Hydrogen Peroxide metabolism, Ketoglutarate Dehydrogenase Complex metabolism, Mitochondria, Muscle metabolism, Superoxides metabolism
- Abstract
Several flavin-dependent enzymes of the mitochondrial matrix utilize NAD(+) or NADH at about the same operating redox potential as the NADH/NAD(+) pool and comprise the NADH/NAD(+) isopotential enzyme group. Complex I (specifically the flavin, site IF) is often regarded as the major source of matrix superoxide/H2O2 production at this redox potential. However, the 2-oxoglutarate dehydrogenase (OGDH), branched-chain 2-oxoacid dehydrogenase (BCKDH), and pyruvate dehydrogenase (PDH) complexes are also capable of considerable superoxide/H2O2 production. To differentiate the superoxide/H2O2-producing capacities of these different mitochondrial sites in situ, we compared the observed rates of H2O2 production over a range of different NAD(P)H reduction levels in isolated skeletal muscle mitochondria under conditions that favored superoxide/H2O2 production from complex I, the OGDH complex, the BCKDH complex, or the PDH complex. The rates from all four complexes increased at higher NAD(P)H/NAD(P)(+) ratios, although the 2-oxoacid dehydrogenase complexes produced superoxide/H2O2 at high rates only when oxidizing their specific 2-oxoacid substrates and not in the reverse reaction from NADH. At optimal conditions for each system, superoxide/H2O2 was produced by the OGDH complex at about twice the rate from the PDH complex, four times the rate from the BCKDH complex, and eight times the rate from site IF of complex I. Depending on the substrates present, the dominant sites of superoxide/H2O2 production at the level of NADH may be the OGDH and PDH complexes, but these activities may often be misattributed to complex I.
- Published
- 2014
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18. Comparative Analysis of the Mitochondrial Physiology of Pancreatic β Cells.
- Author
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Kim C, Patel P, Gouvin LM, Brown ML, Khalil A, Henchey EM, Heuck AP, and Yadava N
- Abstract
The mitochondrial metabolism of β cells is thought to be highly specialized. Its direct comparison with other cells using isolated mitochondria is limited by the availability of islets/β cells in sufficient quantity. In this study, we have compared mitochondrial metabolism of INS1E/β cells with other cells in intact and permeabilized states. To selectively permeabilize the plasma membrane, we have evaluated the use of perfringolysin-O (PFO) in conjunction with microplate-based respirometry. PFO is a protein that binds membranes based on a threshold level of active cholesterol. Therefore, unless active cholesterol reaches a threshold level in mitochondria, they are expected to remain untouched by PFO. Cytochrome c sensitivity tests showed that in PFO-permeabilized cells, the mitochondrial integrity was completely preserved. Our data show that a time-dependent decline of the oligomycin-insensitive respiration observed in INS1E cells was due to a limitation in substrate supply to the respiratory chain. We predict that it is linked with the β cell-specific metabolism involving metabolites shuttling between the cytoplasm and mitochondria. In permeabilized β cells, the Complex l-dependent respiration was either transient or absent because of the inefficient TCA cycle. The TCA cycle insufficiency was confirmed by analysis of the CO
2 evolution. This may be linked with lower levels of NAD+ , which is required as a co-factor for CO2 producing reactions of the TCA cycle. β cells showed comparable OxPhos and respiratory capacities that were not affected by the inorganic phosphate (Pi) levels in the respiration medium. They showed lower ADP-stimulation of the respiration on different substrates. We believe that this study will significantly enhance our understanding of the β cell mitochondrial metabolism.- Published
- 2014
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19. Thiazolidinediones are acute, specific inhibitors of the mitochondrial pyruvate carrier.
- Author
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Divakaruni AS, Wiley SE, Rogers GW, Andreyev AY, Petrosyan S, Loviscach M, Wall EA, Yadava N, Heuck AP, Ferrick DA, Henry RR, McDonald WG, Colca JR, Simon MI, Ciaraldi TP, and Murphy AN
- Subjects
- Acrylates pharmacology, Analysis of Variance, Animals, Anion Transport Proteins, Blotting, Western, Cell Line, Cytochromes c metabolism, Glucose metabolism, Humans, Membrane Potential, Mitochondrial physiology, Mice, Mitochondrial Membrane Transport Proteins, Mitochondrial Proteins metabolism, Monocarboxylic Acid Transporters, Muscle, Skeletal metabolism, Rats, Reverse Transcriptase Polymerase Chain Reaction, Solute Carrier Proteins, Thiazolidinediones metabolism, Cell Respiration drug effects, Membrane Transport Proteins metabolism, Metabolic Networks and Pathways physiology, Mitochondrial Membranes metabolism, Mitochondrial Proteins antagonists & inhibitors, Thiazolidinediones pharmacology
- Abstract
Facilitated pyruvate transport across the mitochondrial inner membrane is a critical step in carbohydrate, amino acid, and lipid metabolism. We report that clinically relevant concentrations of thiazolidinediones (TZDs), a widely used class of insulin sensitizers, acutely and specifically inhibit mitochondrial pyruvate carrier (MPC) activity in a variety of cell types. Respiratory inhibition was overcome with methyl pyruvate, localizing the effect to facilitated pyruvate transport, and knockdown of either paralog, MPC1 or MPC2, decreased the EC50 for respiratory inhibition by TZDs. Acute MPC inhibition significantly enhanced glucose uptake in human skeletal muscle myocytes after 2 h. These data (i) report that clinically used TZDs inhibit the MPC, (ii) validate that MPC1 and MPC2 are obligatory components of facilitated pyruvate transport in mammalian cells, (iii) indicate that the acute effect of TZDs may be related to insulin sensitization, and (iv) establish mitochondrial pyruvate uptake as a potential therapeutic target for diseases rooted in metabolic dysfunction.
- Published
- 2013
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20. Impaired mitochondrial metabolism and mammary carcinogenesis.
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Yadava N, Schneider SS, Jerry DJ, and Kim C
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- Animals, Breast Neoplasms chemically induced, Carcinogens, Environmental toxicity, Disease Susceptibility chemically induced, Disease Susceptibility metabolism, Environmental Exposure adverse effects, Female, Humans, Mammary Glands, Animal drug effects, Mammary Glands, Animal metabolism, Mammary Glands, Human drug effects, Mitochondria drug effects, Oxidative Phosphorylation drug effects, Breast Neoplasms metabolism, Mammary Glands, Human metabolism, Mitochondria metabolism
- Abstract
Mitochondrial oxidative metabolism plays a key role in meeting energetic demands of cells by oxidative phosphorylation (OxPhos). Here, we have briefly discussed (a) the dynamic relationship that exists among glycolysis, the tricarboxylic acid (TCA) cycle, and OxPhos; (b) the evidence of impaired OxPhos (i.e. mitochondrial dysfunction) in breast cancer; (c) the mechanisms by which mitochondrial dysfunction can predispose to cancer; and (d) the effects of host and environmental factors that can negatively affect mitochondrial function. We propose that impaired OxPhos could increase susceptibility to breast cancer via suppression of the p53 pathway, which plays a critical role in preventing tumorigenesis. OxPhos is sensitive to a large number of factors intrinsic to the host (e.g. inflammation) as well as environmental exposures (e.g. pesticides, herbicides and other compounds). Polymorphisms in over 143 genes can also influence the OxPhos system. Therefore, declining mitochondrial oxidative metabolism with age due to host and environmental exposures could be a common mechanism predisposing to cancer.
- Published
- 2013
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21. Mitochondrial dysfunction impairs tumor suppressor p53 expression/function.
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Compton S, Kim C, Griner NB, Potluri P, Scheffler IE, Sen S, Jerry DJ, Schneider S, and Yadava N
- Subjects
- Animals, Base Sequence, Cell Line, Electron Transport Complex I genetics, Gamma Rays, Gene Expression Regulation radiation effects, Mice, Mice, Knockout, Mitochondria genetics, Molecular Sequence Data, Mutation, Neoplasms genetics, Neoplasms metabolism, Oxygen Consumption radiation effects, Tumor Suppressor Protein p53 genetics, Electron Transport Complex I metabolism, Gene Expression Regulation physiology, Mitochondria metabolism, Oxidative Phosphorylation, Oxygen Consumption physiology, Tumor Suppressor Protein p53 metabolism
- Abstract
Recently, mitochondria have been suggested to act in tumor suppression. However, the underlying mechanisms by which mitochondria suppress tumorigenesis are far from being clear. In this study, we have investigated the link between mitochondrial dysfunction and the tumor suppressor protein p53 using a set of respiration-deficient (Res(-)) mammalian cell mutants with impaired assembly of the oxidative phosphorylation machinery. Our data suggest that normal mitochondrial function is required for γ-irradiation (γIR)-induced cell death, which is mainly a p53-dependent process. The Res(-) cells are protected against γIR-induced cell death due to impaired p53 expression/function. We find that the loss of complex I biogenesis in the absence of the MWFE subunit reduces the steady-state level of the p53 protein, although there is no effect on the p53 protein level in the absence of the ESSS subunit that is also essential for complex I assembly. The p53 protein level was also reduced to undetectable levels in Res(-) cells with severely impaired mitochondrial protein synthesis. This suggests that p53 protein expression is differentially regulated depending upon the type of electron transport chain/respiratory chain deficiency. Moreover, irrespective of the differences in the p53 protein expression profile, γIR-induced p53 activity is compromised in all Res(-) cells. Using two different conditional systems for complex I assembly, we also show that the effect of mitochondrial dysfunction on p53 expression/function is a reversible phenomenon. We believe that these findings will have major implications in the understanding of cancer development and therapy.
- Published
- 2011
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22. Quantitative microplate-based respirometry with correction for oxygen diffusion.
- Author
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Gerencser AA, Neilson A, Choi SW, Edman U, Yadava N, Oh RJ, Ferrick DA, Nicholls DG, and Brand MD
- Subjects
- Algorithms, Animals, Cells, Cultured, Diffusion, Fluorescence, Male, Mice, Mitochondria, Liver metabolism, Oxygen Consumption, Synaptosomes metabolism, Oxygen metabolism
- Abstract
Respirometry using modified cell culture microplates offers an increase in throughput and a decrease in biological material required for each assay. Plate based respirometers are susceptible to a range of diffusion phenomena; as O(2) is consumed by the specimen, atmospheric O(2) leaks into the measurement volume. Oxygen also dissolves in and diffuses passively through the polystyrene commonly used as a microplate material. Consequently the walls of such respirometer chambers are not just permeable to O(2) but also store substantial amounts of gas. O(2) flux between the walls and the measurement volume biases the measured oxygen consumption rate depending on the actual [O(2)] gradient. We describe a compartment model-based correction algorithm to deconvolute the biological oxygen consumption rate from the measured [O(2)]. We optimize the algorithm to work with the Seahorse XF24 extracellular flux analyzer. The correction algorithm is biologically validated using mouse cortical synaptosomes and liver mitochondria attached to XF24 V7 cell culture microplates, and by comparison to classical Clark electrode oxygraph measurements. The algorithm increases the useful range of oxygen consumption rates, the temporal resolution, and durations of measurements. The algorithm is presented in a general format and is therefore applicable to other respirometer systems.
- Published
- 2009
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23. Reactive oxygen species regulation by AIF- and complex I-depleted brain mitochondria.
- Author
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Chinta SJ, Rane A, Yadava N, Andersen JK, Nicholls DG, and Polster BM
- Subjects
- Animals, Apoptosis Inducing Factor genetics, Brain ultrastructure, Citrate (si)-Synthase metabolism, Hydrogen Peroxide analysis, Male, Mice, Mitochondria drug effects, Mitochondria genetics, Mutation, Oxidative Stress, Oxygen Consumption drug effects, Oxygen Consumption physiology, Protein Carbonylation drug effects, Protein Carbonylation physiology, Succinic Acid pharmacology, Apoptosis Inducing Factor metabolism, Brain metabolism, Electron Transport Complex I metabolism, Mitochondria metabolism, Reactive Oxygen Species metabolism
- Abstract
Apoptosis-inducing factor (AIF)-deficient harlequin (Hq) mice undergo neurodegeneration associated with a 40-50% reduction in complex I level and activity. We tested the hypothesis that AIF and complex I regulate reactive oxygen species (ROS) production by brain mitochondria. Isolated Hq brain mitochondria oxidizing complex I substrates displayed no difference compared to wild type (WT) in basal ROS production, H2O2 removal, or ROS production stimulated by complex I inhibitors rotenone or 1-methyl-4-phenylpyridinium. In contrast, ROS production caused by reverse electron transfer to complex I was attenuated by approximately 50% in Hq mitochondria oxidizing the complex II substrate succinate. Basal and rotenone-stimulated rates of H2O2 release from in situ mitochondria did not differ between Hq and WT synaptosomes metabolizing glucose, nor did the level of in vivo oxidative protein carbonyl modifications detected in synaptosomes, brain mitochondria, or homogenates. Our results suggest that AIF does not directly modulate ROS release from brain mitochondria. In addition, they demonstrate that in contrast to ROS produced by mitochondria oxidizing succinate, ROS release from in situ synaptosomal mitochondria or from isolated brain mitochondria oxidizing complex I substrates is not proportional to the amount of complex I. These findings raise the important possibility that complex I contributes less to physiological ROS production by brain mitochondria than previously suggested.
- Published
- 2009
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24. Experimental assessment of bioenergetic differences caused by the common European mitochondrial DNA haplogroups H and T.
- Author
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Amo T, Yadava N, Oh R, Nicholls DG, and Brand MD
- Subjects
- Cell Line, Tumor, Genetic Variation, Haplotypes, Humans, Hybrid Cells, Membrane Potential, Mitochondrial, DNA, Mitochondrial metabolism, Oxidative Phosphorylation, Oxygen Consumption, White People genetics
- Abstract
Studies of both survival after sepsis and sperm motility in human populations have shown significant associations with common European mitochondrial DNA haplogroups, and have led to proposals that mitochondria bearing haplogroup H have different bioenergetic capacities than those bearing haplogroup T. However, the validity of such associations assumes that there are no non-random influences of nuclear genes or other factors. Here, we removed the effect of any differences in nuclear genes by constructing transmitochondrial cybrids harbouring mitochondria with either haplogroup H or haplogroup T in cultured A549 human lung carcinoma cells with identical nuclear backgrounds. We compared the bioenergetic capacities and coupling efficiencies of mitochondria isolated from these cells, and of mitochondria retained within the cells, as a critical experimental test of the hypothesis that these haplogroups affect mitochondrial bioenergetics. We found that there were no functionally-important bioenergetic differences between mitochondria bearing these haplogroups, using either isolated mitochondria or mitochondria within cells.
- Published
- 2008
- Full Text
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25. Investigations of the potential effects of phosphorylation of the MWFE and ESSS subunits on complex I activity and assembly.
- Author
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Yadava N, Potluri P, and Scheffler IE
- Subjects
- Amino Acid Sequence, Animals, Cattle, Cell Line, Cricetinae, Electron Transport Complex I chemistry, Membrane Proteins chemistry, Mutation, Phosphorylation, Plasmids, Sequence Alignment, Transfection, Electron Transport Complex I metabolism, Membrane Proteins metabolism, Mitochondria metabolism, Protein Subunits metabolism
- Abstract
There have been several reports on the phosphorylation of various subunits of NADH-ubiquinone oxidoreductase (complex I) in mammalian mitochondria. The effects of phosphorylation on assembly or activity of these subunits have not been investigated directly. The cAMP-dependent phosphorylation of the MWFE and ESSS subunits in isolated bovine heart mitochondria has been recently reported. We have investigated the significance of potential phosphorylation of these two subunits in complex I assembly and function by mutational analysis of the phosphorylation sites. Chinese hamster mutant cell lines missing either the MWFE or the ESSS subunits were transfected and complemented with the corresponding wild type and mutant cDNAs made by site-directed mutagenesis. In MWFE the serine 55 was substituted by alanine, glutamate, glutamine, and aspartate (S55A, S55E, S55Q, and S55D, respectively). The glutamate substitutions might be expected to mimic the phosphorylated state of the protein. With the exception of the MWFE(S55A) mutant protein the assembly of complex I was completely blocked, and no activity could be detected. Various substitutions in the ESSS protein (S2A, S2E, S8A, S8E, T21A, T21E, S30A, S30E) appeared to cause lower levels of mature protein and a significantly reduced complex I activity measured polarographically. The ESSS (S2/8A) double mutant protein caused a complete failure to assemble. These mutational analyses suggest that if phosphorylation occurs in vivo, the effects on complex I activity are significant.
- Published
- 2008
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26. Bioenergetics of mitochondria in cultured neurons and their role in glutamate excitotoxicity.
- Author
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Nicholls DG, Johnson-Cadwell L, Vesce S, Jekabsons M, and Yadava N
- Subjects
- Animals, Cells, Cultured, Humans, Energy Metabolism physiology, Glutamic Acid metabolism, Mitochondria metabolism, Neurons metabolism
- Abstract
The pathologic activation of NMDA receptors by glutamate is a major contributor to neuronal cell death after stroke. Receptor activation causes a massive influx of calcium into the neuron that is accumulated by the mitochondria. The favored hypothesis is that the calcium loaded mitochondria generate reactive oxygen species that damage and ultimately killed the neuron. In this review this hypothesis is critically re-examined with an emphasis on the role played by deficits in ATP generation. Novel techniques are developed to monitor the bioenergetic status of in situ mitochondria in cultured neurons. Applying these techniques to a model of glutamate excitotoxicity suggests that enhanced reactive oxygen species are a consequence rather than a cause of failed cytoplasmic calcium homeostasis (delayed calcium deregulation, [DCD]), but that prior oxidative damage facilitates DCD by damaging mitochondrial ATP generation. This impacts on current hypotheses relating to the neuroprotective effects of mild mitochondrial uncoupling.
- Published
- 2007
- Full Text
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27. Spare respiratory capacity rather than oxidative stress regulates glutamate excitotoxicity after partial respiratory inhibition of mitochondrial complex I with rotenone.
- Author
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Yadava N and Nicholls DG
- Subjects
- Animals, Cell Death drug effects, Cell Death physiology, Cell Respiration drug effects, Cell Respiration physiology, Electron Transport Complex I metabolism, Excitatory Amino Acid Agonists toxicity, Mitochondria drug effects, Oxidative Stress drug effects, Rats, Rats, Wistar, Electron Transport Complex I antagonists & inhibitors, Glutamic Acid toxicity, Mitochondria enzymology, Oxidative Stress physiology, Rotenone pharmacology
- Abstract
Partial inhibition of mitochondrial respiratory complex I by rotenone reproduces aspects of Parkinson's disease in rodents. The hypothesis that rotenone enhancement of neuronal cell death is attributable to oxidative stress was tested in an acute glutamate excitotoxicity model using primary cultures of rat cerebellar granule neurons. As little as 5 nM rotenone increased mitochondrial superoxide (O2*-) levels and potentiated glutamate-induced cytoplasmic Ca2+ deregulation, the first irreversible stage of necrotic cell death. However, the potent cell-permeant O2*- trap manganese tetrakis (N-ethylpyridinium-2yl) porphyrin failed to prevent the effects of the inhibitor. The bioenergetic consequences of rotenone addition were quantified by monitoring cell respiration. Glutamate activation of NMDA receptors used the full respiratory capacity of the in situ mitochondria, and >80% of the glutamate-stimulated respiration was attributable to increased cellular ATP demand. Rotenone at 20 nM inhibited basal and carbonyl cyanide p-trifluoromethoxyphenylhydrazone-stimulated cell respiration and caused respiratory failure in the presence of glutamate. ATP synthase inhibition by oligomycin was also toxic in the presence of glutamate. We conclude that the cell vulnerability in the rotenone model of partial complex I deficiency under these specific conditions is primarily determined by spare respiratory capacity rather than oxidative stress.
- Published
- 2007
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28. Downregulation of NDUFA1 and other oxidative phosphorylation-related genes is a consistent feature of basal cell carcinoma.
- Author
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Mamelak AJ, Kowalski J, Murphy K, Yadava N, Zahurak M, Kouba DJ, Howell BG, Tzu J, Cummins DL, Liégeois NJ, Berg K, and Sauder DN
- Subjects
- Down-Regulation genetics, Electron Transport Complex I, Humans, NADH Dehydrogenase, Oxidative Phosphorylation, RNA, Messenger analysis, Skin Physiological Phenomena genetics, Carcinoma, Basal Cell genetics, Gene Expression Regulation, Neoplastic, Membrane Proteins genetics, Oligonucleotide Array Sequence Analysis, Skin Neoplasms genetics
- Abstract
Basal cell carcinoma (BCC) is the most common cutaneous malignancy that, like other tumours, possesses a heterogeneous genetic composition. In order to select genes with consistent changes in expression among these tumours, we analysed BCC microarray expression data by using a novel approach, termed correlative analysis of microarrays (CAM). CAM is a nested, non-parametric method designed to qualitatively select candidates based on their individual, similar effects upon an array-wide closeness measure. We applied the CAM method to expression data generated by two-channel cDNA microarray experiments, where 21 BCC and patient-matched normal skin specimens were examined. Fifteen candidate genes were selected, with six overexpressed and nine underexpressed in BCC vs. normal skin. Five of the nine consistently downregulated genes in the tumour samples are involved in mitochondrial function and the oxidative phosphorylation (OXPHOS) pathway. One of these genes was the 7.5-kDa subunit, NADH dehydrogenase (ubiquinone) alpha subcomplex-1 (NDUFA1), an accessory component of OXPHOS complex-I that is essential for respiratory activity. These findings support the hypothesis that irregularities in mitochondrial function are involved in neoplasia. Suppression of NDUFA1 expression could represent a key pathogenic mechanism in the development of BCC.
- Published
- 2005
- Full Text
- View/download PDF
29. Molecular genetics of complex I-deficient Chinese hamster cell lines.
- Author
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Scheffler IE, Yadava N, and Potluri P
- Subjects
- Amino Acid Sequence, Animals, Cell Line, Cricetinae, Cricetulus, Cross-Linking Reagents, Electron Transport Complex I metabolism, Mammals metabolism, Membrane Proteins genetics, Membrane Proteins metabolism, Molecular Sequence Data, Mutation, Phosphorylation, Protein Subunits, Protein Transport, Electron Transport Complex I deficiency, Electron Transport Complex I genetics
- Abstract
The work from our laboratory on complex I-deficient Chinese hamster cell mutants is reviewed. Several complementation groups with a complete defect have been identified. Three of these are due to X-linked mutations, and the mutated genes for two have been identified. We describe null mutants in the genes for the subunits MWFE (gene: NDUFA1) and ESSS. They represent small integral membrane proteins localized in the Ialpha (Igamma) and Ibeta subcomplexes, respectively [J. Hirst, J. Carroll, I.M. Fearnley, R.J. Shannon, J.E. Walker. The nuclear encoded subunits of complex I from bovine heart mitochondria. Biochim. Biophys. Acta 1604 (7-10-2003) 135-150.]. Both are absolutely essential for assembly and activity of complex I. Epitope-tagged versions of these proteins can be expressed from a poly-cistronic vector to complement the mutants, or to be co-expressed with the endogenous proteins in other hamster cell lines (mutant or wild type), or human cells. Structure-function analyses can be performed with proteins altered by site-directed mutagenesis. A cell line has been constructed in which the MWFE subunit is conditionally expressed, opening a window on the kinetics of assembly of complex I. Its targeting, import into mitochondria, and orientation in the inner membrane have also been investigated. The two proteins have recently been shown to be the targets for a cAMP-dependent kinase [R. Chen, I.M. Fearnley, S.Y. Peak_Chew, J.E. Walker. The phosphorylation of subunits of complex I from bovine heart mitochondria. J. Biol. Chem. xx (2004) xx-xx.]. The epitope-tagged proteins can be cross-linked with other complex I subunits.
- Published
- 2004
- Full Text
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30. The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase).
- Author
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Potluri P, Yadava N, and Scheffler IE
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cell Respiration genetics, Electron Transport Complex I genetics, Enzyme Stability, Humans, Molecular Sequence Data, Mutation genetics, Oxygen metabolism, Protein Subunits chemistry, Protein Subunits genetics, Cricetinae genetics, Electron Transport Complex I chemistry, Electron Transport Complex I metabolism, Protein Subunits metabolism
- Abstract
The ESSS protein is a recently identified subunit of mammalian mitochondrial complex I. It is a relatively small integral membrane protein (122 amino acids) found in the beta-subcomplex. Genomic sequence database searches reveal its localization to the X-chromosome in humans and mouse. The ESSS cDNA from Chinese hamster cells was cloned and shown to complement one complementation group of our previously described mutants with a proposed X-linkage. Sequence analyses of the ESSS cDNA in these mutants revealed chain termination mutations. In two of these mutants the protein is truncated at the C-terminus of the targeting sequence; the mutants are null mutants for the ESSS subunit. There is no detectable complex I assembly and activity in the absence of the ESSS subunit as revealed by blue native polyacrylamide gel electrophoresis (BN/PAGE) analysis and polarography. Complex I activity can be restored with ESSS subunits tagged with either hemagglutinin (HA) or hexahistidine (His6) epitopes at the C-terminus. Although, the accumulation of ESSS-HA is not dependent upon the presence of mtDNA-encoded subunits (ND1-6,4 L), it is incorporated into complex I only in presence of compatible complex I subunits from the same species.
- Published
- 2004
- Full Text
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31. Disruption of mitochondrial function during apoptosis is mediated by caspase cleavage of the p75 subunit of complex I of the electron transport chain.
- Author
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Ricci JE, Muñoz-Pinedo C, Fitzgerald P, Bailly-Maitre B, Perkins GA, Yadava N, Scheffler IE, Ellisman MH, and Green DR
- Subjects
- Adenosine Triphosphate metabolism, Amino Acid Sequence physiology, Animals, Catalytic Domain genetics, Electron Transport Complex I genetics, Energy Metabolism genetics, HeLa Cells, Humans, Intracellular Membranes metabolism, Intracellular Membranes ultrastructure, Mice, Microscopy, Electron, Mitochondria genetics, Mitochondria ultrastructure, Molecular Sequence Data, Mutation genetics, NADH Dehydrogenase genetics, Reactive Oxygen Species metabolism, Apoptosis physiology, Caspases metabolism, Electron Transport Chain Complex Proteins metabolism, Electron Transport Complex I metabolism, Mitochondria enzymology, NADH Dehydrogenase metabolism
- Abstract
Mitochondrial outer membrane permeabilization and cytochrome c release promote caspase activation and execution of apoptosis through cleavage of specific caspase substrates in the cell. Among the first targets of activated caspases are the permeabilized mitochondria themselves, leading to disruption of electron transport, loss of mitochondrial transmembrane potential (DeltaPsim), decline in ATP levels, production of reactive oxygen species (ROS), and loss of mitochondrial structural integrity. Here, we identify NDUFS1, the 75 kDa subunit of respiratory complex I, as a critical caspase substrate in the mitochondria. Cells expressing a noncleavable mutant of p75 sustain DeltaPsim and ATP levels during apoptosis, and ROS production in response to apoptotic stimuli is dampened. While cytochrome c release and DNA fragmentation are unaffected by the noncleavable p75 mutant, mitochondrial morphology of dying cells is maintained, and loss of plasma membrane integrity is delayed. Therefore, caspase cleavage of NDUFS1 is required for several mitochondrial changes associated with apoptosis.
- Published
- 2004
- Full Text
- View/download PDF
32. Import and orientation of the MWFE protein in mitochondrial NADH-ubiquinone oxidoreductase.
- Author
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Yadava N and Scheffler IE
- Abstract
The MWFE subunit of the mitochondrial NADH-ubiquinone oxidoreductase (complex I) is a small, essential membrane protein of 70 amino acids that is made in the cytosol, imported into mitochondria, and assembled without further proteolytic processing. The experiments identify the first approximately 30 amino acids as a minimal mitochondrial targeting sequence, and establish its orientation in the inner membrane and in complex I. This sequence has a highly conserved glutamate at position 4, which is not typical of a mitochondrial targeting signal. However, it is not essential for MWFE function. Within this sequence there is also a 'stop-transfer' signal. The membrane anchor cannot be replaced by that from another subunit within complex I.
- Published
- 2004
- Full Text
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33. Development and characterization of a conditional mitochondrial complex I assembly system.
- Author
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Yadava N, Houchens T, Potluri P, and Scheffler IE
- Subjects
- Animals, Biochemical Phenomena, Biochemistry, Blotting, Northern, Blotting, Western, Cell Line, Chloramphenicol pharmacology, Cricetinae, DNA, Mitochondrial chemistry, Dose-Response Relationship, Drug, Doxycycline pharmacology, Electron Transport, Electrophoresis, Polyacrylamide Gel, Epitopes chemistry, Fibroblasts metabolism, Gene Deletion, Hemagglutinins metabolism, Kinetics, Membrane Proteins genetics, Mitochondria metabolism, Mutation, Plasmids metabolism, Promoter Regions, Genetic, Protein Binding, Protein Structure, Tertiary, RNA, Messenger metabolism, Reactive Oxygen Species, Time Factors, Electron Transport Complex I physiology
- Abstract
We developed a conditional complex I assembly system in a Chinese hamster fibroblast mutant line, CCL16-B2, that does not express the NDUFA1 gene (encoding the MWFE protein). In this mutant, a hemagglutinin (HA) epitope-tagged MWFE protein was expressed from a doxycycline-inducible promoter. The expression of the protein was absolutely dependent on the presence of doxycycline, and the gene could be turned off completely by removal of doxycycline. These experiments demonstrated a key role of MWFE in the pathway of complex I assembly. Upon induction the MWFE.HA protein reached steady-state levels within 24 h, but the appearance of fully active complex I was delayed by another approximately 24 h. The MWFE appeared in a precomplex that probably includes one or more subunits encoded by mtDNA. The fate of MWFE and the stability of complex I were themselves very tightly linked to the activity of mitochondrial protein synthesis and to the assembly of subunits encoded by mtDNA (ND1-6 and ND4L). This novel conditional system can shed light not only on the mechanism of complex I assembly but emphasizes the role of subunits previously thought of as "accessory." It promises to have broader applications in the study of cellular energy metabolism and production of reactive oxygen species and related processes.
- Published
- 2004
- Full Text
- View/download PDF
34. Species-specific and mutant MWFE proteins. Their effect on the assembly of a functional mammalian mitochondrial complex I.
- Author
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Yadava N, Potluri P, Smith EN, Bisevac A, and Scheffler IE
- Subjects
- Alleles, Amino Acid Sequence, Animals, Blotting, Northern, Blotting, Western, CHO Cells, Cricetinae, DNA, Complementary metabolism, Electron Transport Complex I, Electrophoresis, Polyacrylamide Gel, Gene Deletion, Genes, Dominant, Genetic Complementation Test, Humans, Immunohistochemistry, Mice, Mitochondria metabolism, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutation, NADH Dehydrogenase, Oxygen Consumption, Peptides chemistry, Plasmids metabolism, RNA, Messenger metabolism, Species Specificity, Time Factors, Membrane Proteins chemistry
- Abstract
The MWFE protein (70 amino acids) is highly conserved in evolution, but the human protein (80% identical to hamster) does not complement a null mutation in Chinese hamster cells. We have identified a small protein segment where significant differences exist between rodents and primates, illustrating very specifically the need for compatibility of the nuclear and mitochondrial genomes in the assembly of complex I. The segment between amino acids 39 and 46 appears to be critical for species-specific compatibility. Amino acid substitutions in this region were tested that caused a reduction of activity of the hamster protein or converted the inactive human protein into a partially active one. Such mutations could be useful in making mice with partial complex I activity as models for mitochondrial diseases. Their potential as dominant negative mutants was explored. More deleterious mutations in the NDUFA1 gene were also characterized. A conservative substitution, R50K, or a short C-terminal deletion makes the protein completely inactive. In the absence of MWFE, no high molecular weight complex was detectable by Blue Native-gel electrophoresis. The MWFE protein itself is unstable in the absence of assembled mitochondrially encoded integral membrane proteins of complex I.
- Published
- 2002
- Full Text
- View/download PDF
35. Regulated protein degradation controls PKA function and cell-type differentiation in Dictyostelium.
- Author
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Mohanty S, Lee S, Yadava N, Dealy MJ, Johnson RS, and Firtel RA
- Subjects
- 3',5'-Cyclic-AMP Phosphodiesterases, Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Blotting, Western, Carrier Proteins genetics, Carrier Proteins metabolism, Catalytic Domain, Cell Cycle Proteins genetics, Cyclic AMP metabolism, Cyclic AMP-Dependent Protein Kinases genetics, Dictyostelium genetics, Mitogen-Activated Protein Kinase 1 genetics, Mitogen-Activated Protein Kinase 1 metabolism, Molecular Sequence Data, Morphogenesis, Mutation, Open Reading Frames, Protozoan Proteins genetics, Sequence Alignment, Ubiquitins metabolism, Cell Cycle Proteins metabolism, Cell Differentiation, Cyclic AMP-Dependent Protein Kinases metabolism, Dictyostelium cytology, Dictyostelium enzymology, F-Box Proteins, Gene Expression Regulation, Developmental, Protozoan Proteins metabolism
- Abstract
Cullins function as scaffolds that, along with F-box/WD40-repeat-containing proteins, mediate the ubiquitination of proteins to target them for degradation by the proteasome. We have identified a cullin CulA that is required at several stages during Dictyostelium development. culA null cells are defective in inducing cell-type-specific gene expression and exhibit defects during aggregation, including reduced chemotaxis. PKA is an important regulator of Dictyostelium development. The levels of intracellular cAMP and PKA activity are controlled by the rate of synthesis of cAMP and its degradation by the cAMP-specific phosphodiesterase RegA. We show that overexpression of the PKA catalytic subunit (PKAcat) rescues many of the culA null defects and those of cells lacking FbxA/ChtA, a previously described F-box/WD40-repeat-containing protein, suggesting CulA and FbxA proteins are involved in regulating PKA function. Whereas RegA protein levels drop as the multicellular organism forms in the wild-type strain, they remain high in culA null and fbxA null cells. Although PKA can suppress the culA and fbxA null developmental phenotypes, it does not suppress the altered RegA degradation, suggesting that PKA lies downstream of RegA, CulA, and FbxA. Finally, we show that CulA, FbxA, and RegA are found in a complex in vivo, and formation of this complex is dependent on the MAP kinase ERK2, which is also required for PKA function. We propose that CulA and FbxA regulate multicellular development by targeting RegA for degradation via a pathway that requires ERK2 function, leading to an increase in cAMP and PKA activity.
- Published
- 2001
- Full Text
- View/download PDF
36. Molecular genetics of the mammalian NADH-ubiquinone oxidoreductase.
- Author
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Scheffler IE and Yadava N
- Subjects
- Alleles, Amino Acid Sequence, Animals, Cell Line, Cricetinae, Electron Transport Complex I, Enzyme Stability, Genetic Complementation Test, Humans, Membrane Proteins genetics, Molecular Biology, Molecular Sequence Data, Mutation, NADH Dehydrogenase, NADH, NADPH Oxidoreductases chemistry, Sequence Homology, Amino Acid, NADH, NADPH Oxidoreductases genetics
- Abstract
A serendipitous observation led to the first characterization of a respiration-deficient Chinese hamster mutant cell line. It has guided the design of an enrichment scheme for the isolation of additional mutant cell lines. Several complementation groups were identified with mutations affecting complex I. The X-linked NDUFA1 gene encoding the MWFE protein represents one group. Several mutant alleles isolated independently are described that yield very low activities and demonstrate that the MWFE protein is essential for activity. A phylogenetic sequence analysis of this highly conserved protein has directed attention to species-specific differences that make the primate MWFE protein inactive in hamster cells. Based on such comparisons, mutant alleles made by site-directed mutagenesis were expressed in a null mutant and reduced complex I activities were observed, with the mutant protein assembled into the complex. These and other mutants promise to be valuable for structure-function analyses, especially in conjunction with a high-resolution structure to be expected in the future. The possibility for transgenic and knock-in mice as models for mitochondrial diseases is being explored.
- Published
- 2001
- Full Text
- View/download PDF
37. An F-Box/WD40 repeat-containing protein important for Dictyostelium cell-type proportioning, slug behaviour, and culmination.
- Author
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Nelson MK, Clark A, Abe T, Nomura A, Yadava N, Funair CJ, Jermyn KA, Mohanty S, Firtel RA, and Williams JG
- Subjects
- Amino Acid Motifs, Amino Acid Sequence, Ammonium Chloride pharmacology, Animals, Blotting, Western, Body Patterning genetics, Body Patterning physiology, Dictyostelium cytology, Dictyostelium metabolism, Gene Expression Regulation, Developmental, In Situ Hybridization, Molecular Sequence Data, Mutagenesis, Insertional, Phenotype, Protozoan Proteins isolation & purification, Protozoan Proteins metabolism, Dictyostelium genetics, F-Box Proteins, Protozoan Proteins genetics
- Abstract
FbxA is a novel member of a family of proteins that contain an F-box and WD40 repeats and that target specific proteins for degradation via proteasomes. In fruiting bodies formed from cells where the fbxA gene is disrupted (fbxA(-) cells), the spore mass fails to fully ascend the stalk. In addition, fbxA(-) slugs continue to migrate under environmental conditions where the parental strain immediately forms fruiting bodies. Consistent with this latter behaviour, the development of fbxA(-) cells is hypersensitive to ammonia, the signaling molecule that regulates the transition from the slug stage to terminal differentiation. The slug comprises an anterior prestalk region and a posterior prespore region and the fbxA mRNA is highly enriched in the prestalk cells. The prestalk zone of the slug is further subdivided into an anterior pstA region and a posterior pstO region. In fbxA(-) slugs the pstO region is reduced in size and the prespore region is proportionately expanded. Our results indicate that FbxA is part of a regulatory pathway that controls cell fate decisions and spatial patterning via regulated protein degradation., (Copyright 2000 Academic Press.)
- Published
- 2000
- Full Text
- View/download PDF
38. Calcium binding protein of Entamoeba histolytica.
- Author
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Sahoo N, Chakrabarty P, Yadava N, Bhattacharya S, and Bhattacharya A
- Subjects
- Animals, Calcium-Binding Proteins physiology, Cell Division physiology, Entamoeba histolytica cytology, Calcium-Binding Proteins metabolism, Entamoeba histolytica metabolism
- Published
- 2000
- Full Text
- View/download PDF
39. Episomal expression of specific sense and antisense mRNAs in Leishmania amazonensis: modulation of gp63 level in promastigotes and their infection of macrophages in vitro.
- Author
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Chen DQ, Kolli BK, Yadava N, Lu HG, Gilman-Sachs A, Peterson DA, and Chang KP
- Subjects
- Animals, Base Sequence, In Vitro Techniques, Leishmania mexicana pathogenicity, Macrophages parasitology, Mice, Oligonucleotide Probes genetics, Plasmids genetics, RNA, Antisense genetics, Transcription, Genetic, Transfection, Virulence, Leishmania mexicana enzymology, Leishmania mexicana genetics, Metalloendopeptidases genetics, Metalloendopeptidases metabolism, RNA, Messenger genetics, RNA, Protozoan genetics
- Abstract
The major surface glycoprotein (gp63) of Leishmania amazonensis is a metalloprotease implicated in the infection of mammalian macrophages. The expression of gp63 and its participation in this infection were further examined by modulating the level of this molecule in a virulent gp63-abundant wild-type clone. Promastigotes were transfected with gp63 genes cloned into a Leishmania-specific vector in two different orientations, leading to the expression of gp63 sense and antisense RNAs. With increasing selective pressure, cell surface gp63 was increasingly augmented in the transfectants with sense transcripts and suppressed to a very low level in those with antisense transcripts. Thus, the expression of gp63 from chromosomal, repetitive genes is not stringently regulated at the protein level and can be substantially reduced by episomal antisense transcription of a single copy. The transfectants differed significantly only in the level of gp63, thereby allowing specific evaluation of this molecule in leishmanial infection of macrophages in vitro. Kinetic studies of infection in vitro indicate that gp63 plays a role not only in the binding of this parasite to these macrophages but also in its intramacrophage survival and replication.
- Published
- 2000
- Full Text
- View/download PDF
40. Characterization of EhCaBP, a calcium-binding protein of Entamoeba histolytica and its binding proteins.
- Author
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Yadava N, Chandok MR, Prasad J, Bhattacharya S, Sopory SK, and Bhattacharya A
- Subjects
- Animals, Calcium-Binding Proteins metabolism, Enzyme Activation, Protein Kinases metabolism, Protozoan Proteins metabolism, Calcium-Binding Proteins isolation & purification, Entamoeba histolytica chemistry, Protein Kinases analysis, Protozoan Proteins analysis, Protozoan Proteins isolation & purification
- Abstract
A novel calcium-binding protein (EhCaBP) has been recently identified and characterized from the protozoan parasite Entamoeba histolytica. In order to decipher the function of this protein, a few basic properties were investigated and compared with the ubiquitous Ca(2+)-signal transducing protein calmodulin (CaM). Indirect immunofluorescence and immunoprecipitation analyses using specific antibodies against EhCaBP suggest that it is a soluble cytoplasmic protein with no major post-translational modification. EhCaBP did not stimulate cAMP-phosphodiesterase activity, differentiating it from all known CaMs. Affinity chromatography of [35S]methionine-labelled proteins of E. histolytica trophozoites using EhCaBP-sepharose column showed Ca(2+)-dependent binding of a group of proteins. Radiolabelled proteins from the same extract also bound to CaM-sepharose. However, the proteins bound to the two columns were different as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. At least one of the EhCaBP-binding proteins became phosphorylated as revealed by in vivo phosphorylation analysis. The binding-proteins could not be detected in E. invadens (a species that is pathogenic in reptiles) and E. moshkovskii (which is found in the human gut but is not pathogenic), two species in which EhCaBP-like protein has not been found. Two distinct Ca(2+)-dependent protein kinases, which get activated by EhCaBP and CaM respectively, were detected in E. histolytica. These kinases require different levels of Ca2+ for their maximal activities. Affinity chromatography also showed the binding of protein kinase(s) to EhCaBP in a Ca(2+)-dependent manner. Our data suggest that there may be novel Ca(2+)-signal transduction pathway in E. histolytica mediated by EhCaBP.
- Published
- 1997
- Full Text
- View/download PDF
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