Search

Decoration of an axial coordination ligand (ACL) on the active metal site is a highly effective and versatile strategy to tune activity of single-atom catalysts (SACs). However, the regulation mechanism of ACLs on SACs is still incompletely known. Herein, we investigate diversified combinations of ACL-SACs, including all 3d-5d transition metals and ten prototype ACLs. We identify that ACLs can weaken the adsorption capability of the metal atom (M) by raising the bonding energy levels of the M-O bond while enhancing dispersity of the d orbital of M. Through examination of various local configurations and intrinsic parameters of ACL-SACs, a general structure descriptor σ is constructed to quantify the structure-activity relationship of ACL-SACs which solely based on a few key intrinsic features. Importantly, we also identified the axial ligand descriptor σ ACL , as a part of σ, which can serve as a potential descriptor to determine the rate-limiting steps (RLS) of ACL-SACs in experiment. And we predicted several ACL-SACs, namely, CrN 4 -, FeN 4 -, CoN 4 -, RuN 4 -, RhN 4 -, OsN 4 -, IrN 4 - and PtN 4 -ACLs, that entail markedly higher activities than the benchmark catalysts of Pt and IrO 2 for oxygen reduction reaction and oxygen evolution reaction, respectively, thereby supporting that the general descriptor σ can provide a simple and cost-effective method to assess efficient electrocatalysts., (© 2024 Wiley-VCH GmbH.)

Gold (Au) nanocatalysts present outstanding activity for many reactions and have long attracted much attention, but the size effect of sub-nano-clusters on catalytic activity lacks systematic research. Using CO oxidation as a probe reaction, the size-dependent catalytic capability of sub-nano-Au clusters was explored. The global-minimum (GM) structures of Au N ( N = 2-300, <2.5 nm) were obtained utilizing revised particle swarm optimization (RPSO) combined with density functional theory (DFT) calculations and the Gupta empirical potential. Geometric structural descriptors built a bridge among geometric features, adsorption energy, and the CO oxidation rate of each site of any given sub-nano-Au clusters, making it possible for high-throughput evaluation of the adsorption energy and catalytic activity of the whole sub-nano-Au cluster. The activity per unit mass of sub-nano-Au clusters shows a volcano-shaped relationship with the cluster size, where the sub-nano-Au clusters with a 0.75 nm diameter possess the highest CO 2 formation rate per unit mass. The Edge and Kink sites have a higher turnover frequency (approximately 10 6 ) than the Face sites (approximately 10 2 ), which contribute the most to CO 2 formation. The weak adsorption of CO and O 2 was found to be a crucial factor determining the inferior activity of the Face site to the Kink and Edge sites. The adsorption process rather than the surface reaction step becomes the rate-determining step on the Face site, attributed to the decreased activity per unit mass of sub-nano-Au clusters. This work provides an in-depth mechanistic understanding of size-dependent catalytic activity for Au clusters at the sub-nano level.

The dynamic structure evolution of heterogeneous catalysts during reaction has gained great attention recently. However, controllably manipulating dynamic process and then feeding back catalyst design to extend the lifetime remains challenging. Herein, we proposed an entropy variation strategy to develop a dynamic CuZn-Co/HEOs catalyst, in which the non-active Co nano-islands play a crucial role in controlling thermal effect via timely capturing and utilizing reaction heat generated on the adjacent active CuZn alloys, thus solving the deactivation problem of Cu-based catalysts. Specifically, heat sensitive Co nano-islands experienced an entropy increasing process of slowly redispersion during the reaction. Under such heat dissipation effect, the CuZn-Co/HEOs catalyst exhibited 95.7% ethylene selectivity and amazing long-term stability (>530 h) in the typical exothermic acetylene hydrogenation. Aiming at cultivating it as a catalyst with promising industrial potential, we proposed a simple regeneration approach via an entropy decreasing process., (© 2024 Wiley‐VCH GmbH.)

The highly selective hydrogenation to remove olefins is a significant refining approach for the reformate. Herein, a library of transition metal for reformate hydrogenation is tested experimentally to validate the predictive level of catalytic activity from our theoretical framework, which combines ab initio calculations and microkinetic modeling, with consideration of surface H-coverage effect on hydrogenation kinetics. The favorable H coverage of specific alloy surface under relevant hydrogenation condition, is found to be determined by its corresponding alloy composition. Besides, olefin hydrogenation rate is determined as a function of two descriptors, i.e. H coverage and binding energies of atomic hydrogen, paving the way to computationally screen on metal component in the periodic table. Evaluation of 172 bimetallic alloys based on the activity volcano map, as well as benzene hydrogenation rate, identifies prospective superior candidates and experimentally confirms that Zn 3 Ir 1 outperforms pure Pd catalysts for the selective hydrogenation refining of reformate. The insights into H-coverage-related microkinetic modelling have enabled us to both theoretically understand experimental findings and identify novel catalysts, thus, bridging the gap between first-principle simulations and industrial applications. This work provides useful guidance for experimental catalyst design, which can be easily extended to other hydrogenation reaction., (© 2024 Wiley-VCH GmbH.)

Background: Acne inversa (AI) is a refractory inflammatory skin disease, and TNF-α plays an important role in the pathogenesis of AI. By blocking TNF-α, infliximab (IFX) has been proven to be a promising method., Objectives: To explore the underlying mechanisms of IFX treatment in AI patients., Methods: In this research, we integrated transcriptome sequencing data from the samples of our patients with AI and the GEO database. Ex vivo skin culture of AI patients was conducted to evaluate the efficacy of IFX treatment. Animal studies and cell experiments were used to explore the therapeutic effect and mechanism of IFX treatment., Results: Both TNF-α and NLRP3 inflammasome-related pathways were enriched in skin lesions of AI patients and murine AI models. After IFX treatment, the NLRP3 inflammasome-related pathway was effectively blocked, and the IL-1β level was normalized in ex vivo AI skin explants and murine AI models. Mechanistically, IFX suppressed the NF-κB signaling pathway to lower the expression of NLRP3 and IL-1β in keratinocytes., Conclusions: IFX treatment alleviated skin lesions in murine AI models and downregulated NLRP3 and IL-1β expression levels by inhibiting the NF-κB signaling pathway, which was helpful for understanding the mechanism of IFX therapy., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 Published by Elsevier Ltd.)

Background: White matter injury (WMI) represents a significant etiological factor contributing to neurological impairment subsequent to Traumatic Brain Injury (TBI). CD36 receptors are recognized as pivotal participants in the pathogenesis of neurological disorders, including stroke and spinal cord injury. Furthermore, dynamic fluctuations in the phenotypic polarization of microglial cells have been intimately associated with the regenerative processes within the injured tissue following TBI. Nevertheless, there is a paucity of research addressing the impact of CD36 receptors on WMI and microglial polarization. This investigation aims to elucidate the functional role and mechanistic underpinnings of CD36 in modulating microglial polarization and WMI following TBI., Methods: TBI models were induced in murine subjects via controlled cortical impact (CCI). The spatiotemporal patterns of CD36 expression were examined through quantitative polymerase chain reaction (qPCR), Western blot analysis, and immunofluorescence staining. The extent of white matter injury was assessed via transmission electron microscopy, Luxol Fast Blue (LFB) staining, and immunofluorescence staining. Transcriptome sequencing was employed to dissect the molecular mechanisms underlying CD36 down-regulation and its influence on white matter damage. Microglial polarization status was ascertained using qPCR, Western blot analysis, and immunofluorescence staining. In vitro, a Transwell co-culture system was employed to investigate the impact of CD36-dependent microglial polarization on oligodendrocytes subjected to oxygen-glucose deprivation (OGD)., Results: Western blot and qPCR analyses revealed that CD36 expression reached its zenith at 7 days post-TBI and remained sustained at this level thereafter. Immunofluorescence staining exhibited robust CD36 expression in astrocytes and microglia following TBI. Genetic deletion of CD36 ameliorated TBI-induced white matter injury, as evidenced by a reduced SMI-32/MBP ratio and G-ratio. Transcriptome sequencing unveiled differentially expressed genes enriched in processes linked to microglial activation, regulation of neuroinflammation, and the TNF signaling pathway. Additionally, bioinformatics analysis pinpointed the Traf5-p38 axis as a critical signaling pathway. In vivo and in vitro experiments indicated that inhibition of the CD36-Traf5-MAPK axis curtailed microglial polarization toward the pro-inflammatory phenotype. In a Transwell co-culture system, BV2 cells treated with LPS + IFN-γ exacerbated the damage of post-OGD oligodendrocytes, which could be rectified through CD36 knockdown in BV2 cells., Conclusions: This study illuminates that the suppression of CD36 mitigates WMI by constraining microglial polarization towards the pro-inflammatory phenotype through the down-regulation of the Traf5-MAPK signaling pathway. Our findings present a potential therapeutic strategy for averting neuroinflammatory responses and ensuing WMI damage resulting from TBI., (© 2024. The Author(s).)

Enterotoxigenic Escherichia coli (ETEC) is recognized globally as a major gastrointestinal pathogen that impairs intestinal function. ETEC infection can lead to oxidative stress and disruption of intestinal integrity. The present study investigated the mechanism of increased oxidative stress and whether restoration of antioxidant defense could improve intestinal integrity in a piglet model with ETEC infection. Weaned piglets were divided into three groups: control, ETEC-infection and ETEC-infection with antibiotic supplementation. The infection caused a significant elevation of serum diamine oxidase activity and D-lactate levels coupled with a reduced intestinal (mid-jejunum) tight-junction protein expression, suggesting increased intestinal permeability and impaired gut function. The infection also inhibited nuclear factor erythroid 2-related factor 2 (Nrf2) activation, decreased the expression of glutathione synthesizing enzymes, superoxide dismutase-1 (SOD1), and heme oxygenase-1 (HO-1) in the intestine. This led to a decreased antioxidant glutathione level and an increased lipid peroxidation in the intestine and serum, indicating oxidative stress. The infection stimulated the expression of pro-inflammatory cytokines (IL-6, TNF-α). Antibiotic supplementation attenuated oxidative stress, in part, through restoration of glutathione levels and antioxidant enzyme expression in the intestine. Such a treatment enhanced tight-junction protein expression and improved intestinal function. Furthermore, induction of oxidative stress in Caco2 cells by hydrogen peroxide inhibited tight-junction protein expression and stimulated inflammatory cytokine expression. Glutathione supplementation effectively attenuated oxidative stress and restored tight-junction protein expression. These results suggest that downregulation of Nrf2 activation may weaken antioxidant defense and increase oxidative stress in the intestine. Mitigation of oxidative stress can improve intestinal function after infection., Competing Interests: Declaration of competing interest All authors declare that they have no conflicts of interest., (Copyright © 2024. Published by Elsevier B.V.)

Background: Urosepsis is a serious complication after percutaneous nephrolithotomy (PCNL). This study aimed to develop and validate a nomogram model that can effectively predict urosepsis following PCNL., Methods: A total of 839 patients who underwent PCNL at General Hospital of Southern Theater Command from January 2018 to January 2023 and a total of 609 patients who underwent PCNL at Guangdong Second Provincial General Hospital from January 2020 to January 2023 were retrospectively analyzed in this study. The center with 839 patients was used to develop the model, and another center with 609 patients was used as an external validation group. Multivariate analysis was used to determine the optimal variables. The validation of the nomogram was assessed using the receiver operating characteristic (ROC) curve, calibration curve and decision curve analysis (DCA)., Results: Urosepsis was observed in 47 (5.6%) and 33 (5.4%) patients in the two centers. Four variables were selected to establish the nomogram through multivariate analysis, including operative time [P<0.001, odds ratio (OR): 1.035, 95% confidence interval (CI): 1.019-1.051], accumulated time of renal pelvic pressure ≥30 mmHg (0 vs. 0-60 s, P=0.011, OR: 3.180, 95% CI: 1.300-7.780; 0-60 vs. ≥60 s, P<0.001, OR: 6.389, 95% CI: 2.603-15.685), bladder urine culture (P<0.001, OR: 6.045, 95% CI: 2.454-14.891) and hydronephrosis (none or light vs. moderate, P=0.003, OR: 3.403, 95% CI: 1.509-7.674; moderate vs. several, P=0.002, OR: 4.704, 95% CI: 1.786-12.391). The calibration results showed that the model was well calibrated and ROC curve demonstrated excellent discrimination of the nomogram. In addition, the DCA showed that the nomogram had a positive net benefit., Conclusions: A prediction nomogram was developed and validated to assist clinicians in assessing the probability of urosepsis after PCNL., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://tau.amegroups.com/article/view/10.21037/tau-23-616/coif). The authors have no conflicts of interest to declare., (2024 Translational Andrology and Urology. All rights reserved.)

During the bacterial selection, isolate PF9 demonstrated tolerance to low pH and high bile salt and an ability to extend the lifespan of Caenorhabditis elegans infected with enterotoxigenic Escherichia coli ( ETEC ; P  < 0.05). Thirty-two weaned piglets susceptible to ETEC F4 were randomly allocated to four treatments as follows: 1) non-challenged negative control group ( NNC ; basal diet and piglets gavaged with phosphate-buffered saline), 2) negative control group ( NC ; basal diet and piglets challenged with ETEC F4, 3 × 10 7 CFU per pig), 3) positive control ( PC ; basal diet + 80 mg·kg -1 of avilamycin and piglets challenged with ETEC F4), and 4) probiotic candidate (PF9; control basal diet + 2.5 × 10 9 CFU·kg -1 diet of B. licheniformis PF9 and piglets challenged with ETEC F4). The infection of ETEC F4 decreased average daily gain and gain:feed in the NC group when compared to the NNC group ( P  < 0.05). The inoculation of ETEC F4 induced severe diarrhea at 3 h postinoculum ( hpi ), 36, 40 hpi in the NC group when compared to the NNC group ( P  < 0.05). The supplementation of B. licheniformis PF9 significantly relieved diarrhea severity at 3 hpi when compared to the NC group ( P  < 0.05). The inoculation of ETEC F4 reduced duodenal, jejunal, and ileal villus height ( VH ) in the NC group when compared to the NNC group. A significant ( P  < 0.05) decrease was detected in the duodenal VH in the PC and NNC groups. Moreover, the NNC group had a reduced relative mRNA level of Na + -glucose cotransporter 1 ( SGLT1 ) when compared to the NC group ( P  < 0.05). Compared to the NC and NNC groups, the supplementation of B. licheniformis PF9 increased the relative mRNA levels of aminopeptidase N, occludin, zonula occludens-1, and SGLT1 ( P  < 0.05). The supplementation of B. licheniformis PF9 also significantly increased the relative mRNA level of excitatory amino acid transporter 1 when compared to the NC group ( P  < 0.05). Piglets supplemented with B. licheniformis PF9 showed lower relative abundance of Bacteroidetes in the colon than piglets from the NNC group ( P  < 0.05). The NNC group had a higher relative abundance of Firmicutes in the ileum than all the challenged piglets ( P  < 0.05); however, a lower relative abundance of Proteobacteria in the ileum and colon was observed in the NC group ( P  < 0.05). This study provides evidence that B. licheniformis PF9 has the potential to improve the gut health of piglets under challenging conditions., Competing Interests: All authors declare that they have no conflict of interest., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Objective: To study the biomechanical characteristics of each tissue structure when using different 3D printing Cage in osteoporotic patients undergoing interbody fusion., Methods: A finite element model of the lumbar spine was reconstructed and validated with regarding a range of motion and intervertebral disc pressure from previous in vitro studies. Cage and pedicle screws were implanted and part of the lamina, spinous process, and facet joints were removed in the L4/5 segment of the validated mode to simulate interbody fusion. A 280 N follower load and 7.5 N·m moment were applied to different postoperative models and intact osteoporotic model to simulate lumbar motion. The biomechanical characteristics of different models were evaluated by calculating and analyzing the range of motion of the fixed and cephalic adjacent segment, the stress of the screw-rod system, the stress at the interface between cage and L5 endplate, and intervertebral disc pressure of the adjacent segment., Results: After rigid fixation, the range of motion of the fixed segment of model A-C decreased significantly, which was much smaller than that of the osteoporotic model. And with the increase of the axial area of the interbody fusion cages, the fixed segment of model A-C tended to be more stable. The range of motion and intradiscal pressure of the spinal models with different interbody fusion cages were higher than those of the complete osteoporosis model, but there was no significant difference between the postoperative models. On the other hand, the L5 upper endplate stress and screw-rod system stress of model A-C show a decreasing trend in different directions of motion. The stress of the endplate is the highest during flexion, which can reach 40.5 MPa (model A). The difference in endplate stress between models A-C was the largest during lateral bending. The endplate stress of models A and B was 150.5% and 140.9% of that of model C, respectively. The stress of the screw-rod system was the highest during lateral bending (model A, 102.0 MPa), which was 108.4%, 102.4%, 110.4%, 114.2% of model B and 158.5%, 110.1%, 115.8%, 125.4% of model C in flexion, extension, lateral bending, and rotation, respectively., Conclusions: For people with osteoporosis, no matter what type of cage is used, good immediate stability can be achieved after surgery. Larger cage sizes provide better fixation without significantly increasing ROM and IDP in adjacent segments, which may contribute to the development of ASD. In addition, larger cage sizes can disperse endplate stress and reduce stress concentration, which is of positive significance in preventing cage subsidence after operation. The cage and screw rod system establish a stress conduction pathway on the spine, and a larger cage greatly enhances the stress-bearing capacity of the front column, which can better distribute the stress of the posterior spine structure and the stress borne by the posterior screw rod system, reduce the stress concentration phenomenon of the nail rod system, and avoid exceeding the yield strength of the material, resulting in the risk of future instrument failure., (© 2024. The Author(s).)

Background: The treatment of acne vulgaris is often challenging due to the antibiotic resistance frequently observed in Cutibacterium acnes (C.acnes), a prevalent bacterium linked to this condition., Objective: The objective of this research was to examine the impact of curcumin photodynamic therapy (PDT) on the survival of C.acnes and activity of biofilms produced by this microorganism., Methods: Following the Clinical and Laboratory Standards Institute (CLSI) guidelines, we assessed the drug sensitivity of 25 clinical C.acnes strains to five antibiotics (erythromycin, clindamycin, tetracycline, doxycycline, minocycline) and curcumin by implementing the broth microdilution technique. In addition, we established C.acnes biofilms in a laboratory setting and subjected them to curcumin-PDT(curcumin combined with blue light of 180 J/cm 2 ). Afterwards, we evaluated their viability using the XTT assay and observed them using confocal laser scanning microscopy., Results: The result revealed varying resistance rates among the tested antibiotics and curcumin, with erythromycin, clindamycin, tetracycline, doxycycline, minocycline, and curcumin exhibiting resistance rates of 72 %, 44 %, 36 %, 28 %, 0 %, and 100 %, respectively. In the curcumin-PDT inhibition tests against four representative antibiotic-resistant strains, it was found that the survival rate of all strains of planktonic C. acnes was reduced, and the higher the concentration of curcumin, the lower the survival rate. Furthermore, in the biofilm inhibition tests, the vitality and three-dimensional structure of the biofilms were disrupted, and the inhibitory effect became more significant with higher concentrations of curcumin., Conclusion: The results emphasize the possibility of using curcumin PDT as an alternative approach for the treatment of C.acnes, especially in instances of antibiotic-resistant variations and infections related to biofilms., Competing Interests: Declaration of Competing Interest The authors have no conflicts of interest to declare., (Copyright © 2023. Published by Elsevier B.V.)

Acne is a chronic inflammatory skin disease, and the pathogenesis of acne induced by Cutibacterium acnes (C.acnes) is not well understood. Recently, circular RNAs (circRNAs) have attracted much attention because of its involvement in various diseases. However, the mechanisms by which circRNAs regulated acne have rarely been reported. We identified several differentially expressed circRNAs by sequencing patient-derived acne tissues. Among them, hsa&#95;circ&#95;0105040 was determined to be low expressed in acne tissues and localized in the cytoplasm of human primary keratinocytes. We established a C.acnes biofilms model of acne in vitro and showed that hsa&#95;circ&#95;0105040 promoted inflammation via MAPK and NF-κB pathway. Mechanistically, hsa&#95;circ&#95;0105040 could directly bind to miR-146a and inhibit the expression of miR-146a. Moreover, hsa&#95;circ&#95;0105040 promoted the expression of IRAK1 and TRAF6 by sponging miR-146a, thereby elevating the level of inflammation in acne. Collectively, our data suggested that hsa&#95;circ&#95;0105040- miR-146a -IRAK1/TRAF6 axis was involved in regulating the inflammatory response in acne, which provided a potential therapeutic target for acne and a novel insight into the pathogenesis of inflammatory acne., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Background: Tumor-associated inflammation suggests that anti-inflammatory medication could be beneficial in cancer therapy. Loratadine, an antihistamine, has demonstrated improved survival in certain cancers. However, the anticancer mechanisms of loratadine in lung cancer remain unclear., Objective: This study investigates the anticancer mechanisms of loratadine in lung cancer., Methods: A retrospective cohort of 4,522 lung cancer patients from 2006 to 2018 was analyzed to identify noncancer drug exposures associated with prognosis. Cellular experiments, animal models, and RNA-seq data analysis were employed to validate the findings and explore the antitumor effects of loratadine., Results: This retrospective study revealed a positive association between loratadine administration and ameliorated survival outcomes in lung cancer patients, exhibiting dose dependency. Rigorous in vitro and in vivo assays demonstrated that apoptosis induction and epithelial-mesenchymal transition (EMT) reduction were stimulated by moderate loratadine concentrations, whereas pyroptosis was triggered by elevated dosages. Intriguingly, loratadine was found to augment PPARγ levels, which acted as a gasdermin D transcription promoter and caspase-8 activation enhancer. Consequently, loratadine might incite a sophisticated interplay between apoptosis and pyroptosis, facilitated by the pivotal role of caspase-8., Conclusion: Loratadine use is linked to enhanced survival in lung cancer patients, potentially due to its role in modulating the interplay between apoptosis and pyroptosis via caspase-8., (© 2023. The Author(s).)

Competing Interests: There are no conflicts of interest.

Background: Long noncoding RNAs (lncRNAs) are associated with many dermatologic diseases. However, little is known about the regulatory function of lncRNAs in familial acne inversa (AI) patients with nicastrin (NCSTN) mutation., Objectives: The aim of this study was to explore the regulatory function of lncRNAs in familial AI patients with NCSTN mutation., Methods: The expression profiles of lncRNAs and mRNAs in skin tissues from familial AI patients with NCSTN mutation and healthy individuals were analysed in this study via RNA sequencing (RNA-seq)., Results: In total, 359 lncRNAs and 1,863 mRNAs were differentially expressed between the two groups. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the dysregulated mRNAs targeted by lncRNAs were mainly associated with the immune regulation, Staphylococcus aureus infection and B cell receptor signalling pathways. The lncRNA-miRNA-mRNA coexpression network contained 265 network pairs comprising 55 dysregulated lncRNAs, 11 miRNAs, and 74 mRNAs. Conservation analysis of the differentially expressed lncRNAs between familial AI patients with NCSTN mutation and Ncstn keratinocyte-specific knockout (NcstnΔKC) mice identified 6 lncRNAs with sequence conservation; these lncRNAs may participate in apoptosis, proliferation, and skin barrier function., Conclusions: These findings provide a direction for exploring the regulatory mechanisms underlying the progression of familial AI patients with NCSTN mutation., (© 2023 S. Karger AG, Basel.)

Using Mendelian Randomization (MR) and large-scale Genome-Wide Association Study (GWAS) data, this study aimed to investigate the potential causative relationship between testosterone and sex hormone-binding globulin (SHBG) levels and the onset of several cancers, including pathway enrichment analyses of single nucleotide polymorphisms (SNPs) associated with cancer allowed for a comprehensive bioinformatics approach, which offered a deeper biological understanding of these relationships. The results indicated that increased testosterone levels in women were associated with a higher risk of breast and cervical cancers but a lower risk of ovarian cancer. Conversely, increased testosterone was linked to lower stomach cancer risk for men, whereas high SHBG levels were related to decreased risks of breast and prostate cancers. The corresponding genes of the identified SNPs, as revealed by pathway enrichment analysis, were involved in significant metabolic and proliferative pathways. These findings emphasize the need for further research into the biological mechanisms behind these associations, paving the way for potential targeted interventions in preventing and treating these cancers.

Carbon-based support anchored 3d transition metal (TM) single atom catalysts (SACs) have been widely considered as promising candidates for the oxygen reduction reaction (ORR), and their intrinsic activity is closely related to the geometric and electronic structures of the supports. T-graphene was predicted to have high conductivity and stability, so it may be also a promising support for loading SACs for electrocatalysis. Here, we systematically evaluate the ORR activity of T-graphene supported TM single atoms (TMN 4 -Tgra and TMC 4 -Tgra, TM = Sc-Zn) and their graphene supported counterparts (TMN 4 -Gra and TMC 4 -Gra). The TM(d xz )-O(p x ), TM(d yz )-O(p y ) and TM(d z 2 )-O(p z + s) orbital hybridizations between the active central metal and the *OH intermediate determine the ORR activity. Compared to graphene, T-graphene increases the d-band center (especially the β-spin state) of single atoms and reduces the *OH adsorption strength, which thus improves the ORR activity of the catalysts located in the left leg of the ORR activity volcano plot. Interestingly, we found that for the catalysts with different TMs anchored on the same support, the adsorption strength of oxygen intermediates increases with the increase of the d-band center of the active site, while for the catalysts with the same metal anchored on different supports, the adsorption strength of oxygen intermediates weakens with the increase of the d-band center of the active site, which can serve as a d-band center dependence law on supports and active sites for designing ORR catalysts.

Recently, a lot of attention has been dedicated to double- or triple-atom catalysts (DACs/TACs) as promising alternatives to platinum-based catalysts for the oxygen reduction reaction (ORR) in fuel cell applications. However, the ORR activity of DACs/TACs is usually theoretically understood or predicted using the single-site association pathway (O 2 → OOH* → O* → OH* → H 2 O) proposed from Pt-based alloy and single-atom catalysts (SACs). Here, we investigate the ORR process on a series of graphene-supported Fe-Co DACs/TACs by means of first-principles calculation and an electrode microkinetic model. We propose that a dual channel for electron acceptance-backdonation on adjacent metal sites of DACs/TACs efficiently promotes O-O bond breakage compared with SACs, which makes ORR switch to proceed through dual-site dissociation pathways (O 2 → O* + OH* → 2OH* → OH* → H 2 O) from the traditional single-site association pathway. Following this revised ORR network, a complete reaction phase diagram of DACs/TACs is established, where the preferential ORR pathways and activity can be described by a three-dimensional volcano plot spanned by the adsorption free energies of Δ G (O*) and Δ G (OH*). Besides, the kinetics preferability of dual-site dissociation pathways is also appropriate for other graphene- or oxide-supported DACs/TACs. The contribution of dual-site dissociation pathways, rather than the traditional single-site association pathway, makes the theoretical ORR activity of DACs/TACs in better agreement with available experiments, rationalizing the superior kinetic behavior of DACs/TACs to that of SACs. This work reveals the origin of ORR pathway switching from SACs to DACs/TACs, which broadens the ideas and lays the theoretical foundation for the rational design of DACs/TACs and may also be heuristic for other reactions catalyzed by DACs/TACs., Competing Interests: The authors declare no competing financial interest., (© 2023 The Authors. Published by American Chemical Society.)

Objective: Intrathyroidal thymic carcinoma (ITC) is a rare low-grade malignant thyroid tumor. There is neither sufficient understanding of this tumor nor its clinical treatment. This study is to explore the clinicopathological features, treatment, and prognosis of ITC and thereby provide a reference for the diagnosis and treatment of the disease., Methods: The clinical, pathological, therapeutic, and prognostic data of 13 patients with ITC were retrospectively analyzed., Results: The case series comprised 7 males and 6 females, with an average age of 51.9 ± 10.1 years. After surgical resection, all patients received post-operative neck radiotherapy at dosages of 60-66 Gy. Five patients with level VI lymph node metastasis additionally received 6 courses of cisplatin chemotherapy. All patients were followed-up for 21-132 months (median = 66 months), and all of them survived without recurrence or metastasis., Conclusions: The diagnosis of ITC depends mainly on pathological and immunohistochemical results, particularly CD5 positive staining. Surgical resection is the preferred primary treatment modality which can be supplemented with radiotherapy and chemotherapy to reduce the risk of recurrence and metastasis.

Traumatic brain injury (TBI), particularly diffuse axonal injury (DAI), often results in sympathetic hyperactivity, which can exacerbate the prognosis of TBI patients. A key component of this process is the role of neutrophils in causing neuroinflammation after TBI by forming neutrophil extracellular traps (NETs), but the connection between NETs and sympathetic excitation following TBI remains unclear. Utilizing a DAI rat model, the current investigation examined the role of NETs and the HMGB1/JNK/AP1 signaling pathway in this process. The findings revealed that sympathetic excitability intensifies and peaks 3 days post-injury, a pattern mirrored by the activation of microglia, and the escalated NETs and HMGB1 levels. Subsequent in vitro exploration validated that HMGB1 fosters microglial activation via the JNK/AP1 pathway. Moreover, in vivo experimentation revealed that the application of anti-HMGB1 and AP1 inhibitors can mitigate microglial M1 polarization post-DAI, effectively curtailing sympathetic hyperactivity. Therefore, this research elucidates that post-TBI, NETs within the PVN may precipitate sympathetic hyperactivity by stimulating M1 microglial polarization through the HMGB1/JNK/AP1 pathway., (© 2023 Federation of American Societies for Experimental Biology.)

Background: Previous observational studies have found an association between gastroesophageal reflux disease (GERD) and chronic respiratory diseases, but it remains uncertain whether GERD causally influences these diseases. In this study, we aimed to estimate the causal associations between GERD and 5 chronic respiratory diseases., Methods: 88 GERD-associated single nucleotide polymorphisms (SNPs) identified by the latest genome-wide association study were included as instrumental variables. Individual-level genetic summary data of participants were obtained from corresponding studies and the FinnGen consortium. We applied the inverse-variance weighted method to estimate the causality between genetically predicted GERD and 5 chronic respiratory diseases. Furthermore, the associations between GERD and common risk factors were investigated, and mediation analyses were conducted using multivariable MR. Various sensitivity analyses were also performed to verify the robustness of the findings., Results: Our study demonstrated that genetically predicted GERD was causally associated with an increased risk of asthma (OR 1.39, 95%CI 1.25-1.56, P < 0.001), idiopathic pulmonary fibrosis (IPF) (OR 1.43, 95%CI 1.05-1.95, P = 0.022), chronic obstructive disease (COPD) (OR 1.64, 95%CI 1.41-1.93, P < 0.001), chronic bronchitis (OR 1.77, 95%CI 1.15-2.74, P = 0.009), while no correlation was observed for bronchiectasis (OR 0.93, 95%CI 0.68-1.27, P = 0.645). Additionally, GERD was associated with 12 common risk factors for chronic respiratory diseases. Nevertheless, no significant mediators were discovered., Conclusions: Our study suggested that GERD was a causal factor in the development of asthma, IPF, COPD and chronic bronchitis, indicating that GERD-associated micro-aspiration of gastric contents process might play a role in the development of pulmonary fibrosis in these diseases., (© 2023. The Author(s).)

Background: There are limited data on the in vivo natural kinematics of the lumbar spinous process. This paper intends to explore the effect of lifting load on the in vivo movement mode of the lumbar spinous process and its biomechanical changes., Methods: Ten asymptomatic subjects between the ages of 25 and 39 underwent CT scans of the lumbar spine in the supine position, and 3D models of L3-L5 were constructed. Using a Dual Fluoroscopy Imaging System (DFIS), instantaneous orthogonal fluoroscopic images of each subject's flexion-extension, left-right bending, and left-right rotational movements were taken under different loads (0 kg, 5 kg, 10 kg). The supine CT model was matched, using computer software, to the bony contours of the images from the two orthogonal views, so that the instantaneous 3D vertebral position at each location could be quantified. A Cartesian coordinate system was ultimately constructed at the tip of the spinous process to obtain the 6DOF kinematic data of the spinous process., Results: In different postural movements of the trunk, there was no significant difference in the rotation angle and translation range of the lumbar spinous process under different loads (P > 0.05). In flexion to extension motion, spinous processes mainly rotate < 4° along the medial and lateral axes and translate < 4 mm along the craniocaudal direction. In the left-right bending motion, spinous processes mainly rotate < 5° along the anterior and posterior axes, and the translation is mainly coupling < 2 mm. In the rotational motion, the spinous process is mainly coupled motion, the rotation range is less than 3°, and the translation range is less than 2 mm. The distance between spinous processes measured in the supine position was 6.66 ± 2.29 mm at L3/4 and 5.08 ± 1.57 mm at L4/5., Conclusion: The in vivo kinematics of the lumbar spinous process will not change significantly with increasing low load. In complex motion, the spinous process is dominated by coupling motion., (© 2023. The Author(s).)

The composition effect of PdPt alloys on preferential hydrogenation of C 6 olefins over benzene is studied by combining density functional theory calculations and microkinetic modeling. A trade-off between activity and selectivity is found with increasing Pt component. Pd 3 Pt 1 is identified with high selectivity (low aromatic depletion), while Pd 1 Pt 1 and Pd 1 Pt 3 are more active for olefin hydrogenation. The PdPt alloys present superior sulfur tolerance compared to Pd.

Background: Pelvic parameters have been taken into consideration for the evaluation of the outcomes of bracing in AIS. To discuss the stress required to correct the pelvic deformity related to Lenke5 adolescent idiopathic scoliosis (AIS) by finite element analysis, and provide a reference for the shaping of the pelvic region of the brace., Methods: An three-dimensional (3D) corrective force on the pelvic area was defined. Computed tomography images were used to reconstruct a 3D model of Lenke5 AIS. Computer-aided engineering software Abaqus was used to implement finite element analysis. By adjusting the magnitude and position of corrective forces, coronal pelvic coronal plane rotation (PCPR) and Cobb angle (CA) of lumbar curve in the coronal plane, horizontal pelvic axial plane rotation, and apical vertebra rotation (AVR) were minimized to achieve the best effect on the spine and pelvic deformity correction. The proposed corrective conditions were divided into three groups: (1) forces applied on X-axis; (2) forces applied both in the X- and Y-axis; and (3) forces applied along the X-, Y-, and Z-axis at the same time., Results: In three groups, CA correction reduced by 31.5%, 42.5%, and 59.8%, and the PCPR changed to 12°, 13°, and 1° from 6.5°, respectively. The best groups of correction forces should simultaneously locate on the sagittal, transverse, and coronal planes of the pelvis., Conclusions: For Lenke5 AIS, 3D correction forces can sufficiently reduce scoliosis and pelvic asymmetrical state. Force applied along the Z-axis is vital to correct the pelvic coronal pelvic tilt associated with Lenke5 AIS., (© 2023. The Author(s).)

Objective: To study the biomechanical characteristics of various tissue structures of different sizes of 3D printed Cage in lumbar interbody fusion., Methods: A finite element model of normal spine was reconstructed and verified. Pedicle screws and Cage of different sizes were implanted in the L4/5 segment to simulate lumbar interbody fusion. The range of motion of the fixed and cephalic adjacent segment, the stress of the screw-rod system, the stress at the interface between cage and L5 endplate, and intervertebral disc pressure of the adjacent segment were calculated and analyzed., Results: The range of motion and intervertebral disc pressure of the adjacent segment of each postoperative model were larger than those of the intact model, but there was not much difference between them. The stress of cage-endplate interface was also larger than that of the intact model. However, the difference is that the stress of the endplate and the screw-rod system has a tendency to decrease with the increase of the axial area of cage., Conclusions: Cage with larger axial area in lumbar interbody fusion can reduce the stress of internal fixation system and endplate, but will not increase the range of motion and intervertebral disc pressure of adjacent segment. It has a certain effect in preventing the cage subsidence, internal fixation system failure and screw rod fracture., (© 2023. The Author(s).)

Basic monitoring of the marine environment is crucial for the early warning and assessment of marine hydrometeorological conditions, climate change, and ecosystem disasters. In recent years, many marine environmental monitoring platforms have been established, such as offshore platforms, ships, or sensors placed on specially designed buoys or submerged marine structures. These platforms typically use a variety of sensors to provide high-quality observations, while they are limited by low spatial resolution and high cost during data acquisition. Satellite remote sensing allows monitoring over a larger ocean area; however, it is susceptible to cloud contamination and atmospheric effects that subject the results to large uncertainties. Unmanned vehicles have become more widely used as platforms in marine science and ocean engineering in recent years due to their ease of deployment, mobility, and the low cost involved in data acquisition. Researchers can acquire data according to their schedules and convenience, offering significant improvements over those obtained by traditional platforms. This study presents the state-of-the-art research on available unmanned vehicle observation platforms, including unmanned aerial vehicles (UAVs), underwater gliders (UGs), unmanned surface vehicles (USVs), and unmanned ships (USs), for marine environmental monitoring, and compares them with satellite remote sensing. The recent applications in marine environments have focused on marine biochemical and ecosystem features, marine physical features, marine pollution, and marine aerosols monitoring, and their integration with other products are also analysed. Additionally, the prospects of future ocean observation systems combining unmanned vehicle platforms (UVPs), global and regional autonomous platform networks, and remote sensing data are discussed., Competing Interests: Declaration of competing interest There are no conflicts of interest to declare., (Copyright © 2022. Published by Elsevier B.V.)

Alloying is an effective approach to improve the catalysis performance of Pd-based catalysts for the selective hydrogenation of diolefins towards monoolefines. Herein, PdAgCu ternary nanoalloy catalysts were synthesised by a stepwise impregnation method for isoprene selective hydrogenation. The addition of a moderate amount of Ag and Cu to Pd significantly enhances the isoamylene selectivity in the isoprene hydrogenation, and decreases the non-desired over-hydrogenation. In addition, the loading molar ratio of PdAgCu with 3 : 2 : 3 as the optimal ternary nanoalloy composition maximizes the isoprene conversion (98%) and the monoolefins yield (92%). The surface structure of the catalyst was probed using H 2 -TPR, TEM, XRD, and XPS characterization methods, and it was confirmed that the surface Pd composition ratio between the metallic and oxidized states shows significant effects on the monoolefines yield. This work demonstrates the advantages of PdAgCu ternary nanoalloy catalysts for isoprene selective hydrogenation, which also provides guidelines for the development of other Pd-based ternary nanoalloys for diolefins selective hydrogenation.

Introduction: CircRNAs are closely related to many human diseases; however, their role in acne remains unclear. This study aimed to determine the role of hsa&#95;circ&#95;0102678 in regulating inflammation of acne., Methods: First, microarray analysis was performed to study the expression of circRNAs in acne. Subsequently, RNase R digestion assay and fluorescence in situ hybridization assay were utilized to confirm the characteristics of hsa&#95;circ&#95;0102678. Finally, qRT-PCR, Western blotting analysis, immunoprecipitation, luciferase reporter assay, circRNA probe pull-down assay, biotin-labeled miRNA pull-down assay, RNA immunoprecipitation assay, and m6A dot blot assay were utilized to reveal the functional roles of hsa&#95;circ&#95;0102678 on inflammation induced by C. acnes biofilm in human primary keratinocytes., Results: Our investigations showed that the expression of hsa&#95;circ&#95;0102678 was significantly decreased in acne tissues, and hsa&#95;circ&#95;0102678 was a type of circRNAs, which was mainly localized in the cytoplasm of primary human keratinocytes. Moreover, hsa&#95;circ&#95;0102678 remarkably affected the expression of IL-8, IL-6, and TNF-α, which induced by C. acnes biofilm. Importantly, mechanistic studies indicated that the YTHDC1 could bind directly to hsa&#95;circ&#95;0102678 and promote the export of N6-methyladenosine-modified hsa&#95;circ&#95;0102678 to the cytoplasm. Besides, hsa&#95;circ&#95;0102678 could bind to miR-146a and sponge miR-146a to promote the expression of IRAK1 and TRAF6., Conclusion: Our findings revealed a previously unknown process by which hsa&#95;circ&#95;0102678 promoted keratinocyte inflammation induced by C. acnes biofilm via regulating miR-146a/TRAF6 and IRAK1 axis., (© 2023 The Author(s). Published by S. Karger AG, Basel.)

Background: At present, research on spinal shortening is mainly focused on the safe distance of spinal shortening and the mechanism of spinal cord injury, but there is no research on the biomechanical characteristics of different shortening distances. The purpose of this study was to study the biomechanical characteristics of spine and internal fixation instruments at different shortening distances by the finite element (FE) method., Methods: An FE model of lumbar L1-S was established and referred to the previous in vitro experiments to verify the rationality of the model by verifying the Intradiscal pressure (IDP) and the range of motion (ROM) of the motion segment. Five element models of spinal shortening were designed under the safe distance of spinal shortening, and the entire L3 vertebra and both the upper and lower intervertebral discs were resected. Model A was not shortened, while models B-E were shortened by 10%, 20%, 30% and 50% of the vertebral body, respectively. Constraining the ROM of the sacrum in all directions, a 7.5 N ·m moment and 280 N follower load were applied on the L1 vertebra to simulate the motion of the lumbar vertebrae in three planes. The ROM of the operated segments, the Von Mises stress (VMS) of the screw-rod system, the VMS of the upper endplate at the interface between the titanium cage and the L4 vertebral body, and the ROM and the IDP of the adjacent segment (L5/S) were recorded and analysed., Results: All surgical models showed good stability at the operated segments (L1-5), with the greatest constraint in posterior extension (99.3-99.7%), followed by left-right bending (97.9-98.7%), and the least constraint in left-right rotation (84.9-86.3%) compared with the intact model. The VMS of the screw-rod system and the ROM and IDP of the distal adjacent segments of models A-E showed an increasing trend, in which the VMS of the screw-rod system of model E was the highest under flexion (172.5 MPa). The VMS of the endplate at the interface between the cage and L4 upper endplate of models A-E decreased gradually, and these trend were the most obvious in flexion, which were 3.03, 2.95, 2.83, 2.78, and 2.61 times that of the intact model, respectively., Conclusion: When performing total vertebrae resection and correcting the spinal deformity, if the corrected spine has met our needs, the distance of spinal shortening should be minimized to prevent spinal cord injury, fracture of internal fixations and adjacent segment disease (ASD)., (© 2022. The Author(s).)

Background: Curcumin has been employed as a photosensitizer agent during photodynamic therapy (PDT). Cutibacterium acnes (C. acnes) can cause an inflammatory response in human keratinocytes; however, no research has been conducted to determine whether curcumin and its photodynamic properties can prevent this inflammatory reaction., Objective: We hypothesized that curcumin may control the C. acnes biofilm-induced inflammatory response in keratinocytes, either alone or in combination with blue light photodynamic therapy., Methods: Following C. acnes biofilm stimulation, human primary keratinocytes were treated with 20 μM curcumin solution alone or 5 μM curcumin with combined blue light irradiation. The amount of secreted protein was measured using an ELISA kit. The expression levels of Toll-like receptor 2 (TLR2) and its downstream proteins were determined using western blot., Results: Treatment with 20 μM curcumin, but not 5 μM curcumin, reduced the inflammatory response to C. acnes biofilms in keratinocytes by blocking the TLR2/MAPK/NF-κB pathway. Interestingly, 5 μM curcumin combined with blue light also reduced the C. acnes biofilm-induced inflammation indicated above by blocking the TLR2/MAPK/NF-κB pathway., Conclusion: Curcumin alone, in sufficient concentrations, or low-concentration curcumin with blue light had anti-inflammatory activity on keratinocytes stimulated by C. acnes biofilms through inhibition of MAPK and NF-κB signaling pathways by downregulating TLR2 expression., Competing Interests: Declaration of Competing Interest The authors have no conflicts of interest to declare., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Nitrate electrochemical reduction to ammonia (NO 3 RR) catalyzed by single-atom catalysts (SACs) is an attractive and efficient way for solving the problem of nitrate pollution in water and obtaining valuable product ammonia through low temperature synthesis. It is well known that the pH conditions can be regulated to tune the performance of NO 3 RR, however, there have been few studies aimed at gaining theoretical insight into the origin of pH-dependent catalytic performance among SACs. Herein, taking 3d-transition metal (Fe, Co, Ni and Mn) single-atoms supported on diverse anchor sites of MoS 2 as an example (SA-MoS 2 ), we explore the activity and selectivity for NO 3 RR towards ammonia (NH 3 and NH 4 + ) under different pH conditions by density functional theory calculations. It is found that priority reaction pathways, the potential determining step and limiting potentials of SA-MoS 2 exhibit pH-dependent characteristics, which can be described by a contour map of catalytic reactivity, spanned by adsorption free energies ( G NO* and G NH 2 * ), and further determined by local coordination environment and electronic states of active sites. Our three-step screening method reveals that the Co single-atom adsorbed MoS 2 edge catalyst is the most promising catalyst among the studied SA-MoS 2 because of its low limiting potential (-0.3-0.4 V, RHE), excellent selectivity in the competition with the hydrogen evolution reaction (HER), as well as stability against aggregation and electrochemical dissolution across the full pH range. This work demonstrates a theoretical insight into the pH-dependent mechanism of supported SA catalyzed NO 3 RR, which proposes a screening strategy for finding new SACs, and provides motivation for further experimental exploration.

To date, the effect of oxidation state on activity remains controversial in whether higher or lower oxidation states benefit the enhancement of catalytic activity. Herein, we discover a volcanic relationship between oxidation state and hydrogen evolution reaction activity based on Os single-atom catalysts. Firstly, a series of Os SACs with oxidation states ranging from  + 0.9 to  + 2.9 are synthesized via modifying the coordination environments, including Os-N 3 S 1 , Os-N 4 , Os-S 6 , Os-C 3 , and Os-C 4 S 2 . A volcano-type relation between oxidation states and hydrogen evolution activity emerge with a summit at a moderate experimental oxidation state of  + 1.3 (Os-N 3 S 1 ). Mechanism studies illustrate that with increasing oxidation states, the adsorption of H atoms on Os is strengthened due to increased energy level and decreased occupancy of anti-bonding states of Os-H bond until the anti-bonding states become empty. Further increasing the oxidation states weakens hydrogen adsorption because of the decreased occupancy of Os-H bonding states. In this work, we emphasize the essential role of oxidation state in manipulating activity, which offers insightful guidance for the rational design of single-atom catalysts., (© 2022. The Author(s).)

Background: Advancements in medicine and the popularity of lumbar fusion surgery have made lumbar adjacent segment disease (ASDz) increasingly common, but there is no mature plan for guiding its surgical treatment. Therefore, in this study, four different finite element (FE) ASDz models were designed and their biomechanical characteristics were analysed to provide a theoretical basis for clinical workers to choose the most appropriate revision scheme for ASDz., Methods: According to whether internal fixation was retained, different FE models were created to simulate ASDz revision surgery, and flexion, extension, axial rotation and lateral bending were simulated by loading. The biomechanical characteristics of the adjacent segments of the intervertebral disc and the internal fixation system and the range of motion (ROM) of the lumbar vertebrae were analysed., Results: The difference in the ROM of the fixed segment between FE models that did or did not retain the original internal fixation was less than 0.1°, and the difference was not significant. However, the stress of the screw-rod system when the original internal fixation was retained and prolonged fixation was performed with dual-trajectory screws was less than that when the original internal fixation was removed and prolonged fixation was performed with a long bar. Especially in axial rotation, the difference between models A and B is the largest, and the difference in peak stress reached 30 MPa. However, for the ASDz revision surgery segment, the endplate stress between the two models was the lowest, and the intradiscal pressure (IDP) of the adjacent segment was not significantly different between different models., Conclusion: Although ASDz revision surgery by retaining the original internal fixation and prolonging fixation with dual-trajectory screws led to an increase in stress in the fusion segment endplate, it provides stability similar to ASDz revision surgery by removing the original internal fixation and prolonging fixation with a long bar and does not lead to a significant change in the IDP of the adjacent segment while avoiding a greater risk of rod fracture., (© 2022. The Author(s).)

Objective: To investigate and evaluate the biomechanical behaviour of tension-band-reconstruction (TBR) and ordinary titanium plates in open-door laminoplasty by finite element (FE) analysis., Methods: TBR titanium plate and ordinary titanium plate were implanted into a validated finite element model of healthy adult cervical vertebrae. Among them, 5 ordinary titanium plate were used in model A, 2 TBR titanium plates and 3 ordinary titanium plates were used in model B, and 5 TBR titanium plates were used in model C. The same loading conditions was applied identically to all models. Range of motion (ROM) of the vertebral body, stress distribution of the titanium plate and intradiscal pressure (IDP) were compared in flexion, extension, lateral bending and rotation., Results: The ROM of model B and C was similar in flexion and extension, and both were smaller than that of model A. The highest von Mises stress in the titanium plate appears is in model C. The IDP in C2/3 was significantly higher than that in other segments in flexion. There was no significant difference in IDP among three models in left lateral bending and left axial rotation., Conclusion: Application of TBR titanium plate in open-door laminoplasty can reduced ROM in flexion, extension and axial rotation of the cervical vertebrae. But the increase of stress in TBR titanium plate could lead to higher risk of adverse events such as titanium plate deformation. Moreover, compared with complete TBR titanium plate, the combination of TBR titanium plate for C3 and C7 with ordinary titanium plate for the other vertebrae largely reduce the stress of the titanium plates by ensuring stability. The proposed FE model (C2-T1) exhibits a great potential in evaluating biomechanical behaviour of TBR titanium plate for open-door laminoplasty., (© 2022. The Author(s).)

The controlled oxidation of alcohols to the corresponding ketones or aldehydes via selective cleavage of the β-C-H bond of alcohols under mild conditions still remains a significant challenge. Although the metal/oxide interface is highly active and selective, the interfacial sites fall far behind the demand, due to the large and thick support. Herein, we successfully develop a unique Au-CuO Janus structure (average particle size=3.8 nm) with an ultrathin CuO layer (0.5 nm thickness) via a bimetal in situ activation and separation strategy. The resulting Au-CuO interfacial sites prominently enhance isopropanol adsorption and decrease the energy barrier of β-C-H bond scission from 1.44 to 0.01 eV due to the strong affinity between the O atom of CuO and the H atom of isopropanol, compared with Au sites alone, thereby achieving ultrahigh acetone selectivity (99.3 %) over 1.1 wt % AuCu 0.75 /Al 2 O 3 at 100 °C and atmospheric pressure with 97.5 % isopropanol conversion. Furthermore, Au-CuO Janus structures supported on SiO 2 , TiO 2 or CeO 2 exhibit remarkable catalytic performance, and great promotion in activity and acetone selectivity is achieved as well for other reducible oxides derived from Fe, Co, Ni and Mn. This study should help to develop strategies for maximized interfacial site construction and structure optimization for efficient β-C-H bond activation., (© 2022 Wiley-VCH GmbH.)

Background: Quantitative data on in vivo vertebral disc deformations are critical for enhancing our understanding of spinal pathology and improving the design of surgical materials. This study investigated in vivo lumbar intervertebral disc deformations during axial rotations under different load-bearing conditions., Methods: Twelve healthy subjects (7 males and 5 females) between the ages of 25 and 39 were recruited. Using a combination of a dual fluoroscopic imaging system (DFIS) and CT, the images of L3-5 segments scanned by CT were transformed into three-dimensional models, which matched the instantaneous images of the lumbar spine taken by a double fluorescent X-ray system during axial rotations to reproduce motions. Then, the kinematic data of the compression and shear deformations of the lumbar disc and the coupled bending of the vertebral body were obtained., Results: Relative to the supine position, the average compression deformation caused by rotation is between + 10% and - 40%, and the shear deformation is between 17 and 50%. Under physiological weightbearing loads, different levels of lumbar discs exhibit similar deformation patterns, and the deformation patterns of left and right rotations are approximately symmetrical. The deformation patterns change significantly under a 10 kg load, with the exception of the L3-4 disc during the right rotation., Conclusion: The deformation of the lumbar disc was direction-specific and level-specific during axial rotations and was affected by extra weight. These data can provide new insights into the biomechanics of the lumbar spine and optimize the parameters of artificial lumbar spine devices., (© 2022. The Author(s).)

Background: Lumbar facet joints (LFJs) are usually related to the pathogenesis of the spine. The purpose of this paper is to study the effects of lifting load on the motion of lower lumbar facet joints in vivo., Methods: Ten healthy volunteers aged 25 ≤ 39 years, 5 males and 5 females, were recruited. Using a dual fluoroscopy imaging system (DFIS) combined with CT, firstly, the L3-S1 segment image scanned by CT was converted into a three-dimensional model. Then, the lumbar motion images of L3-S1 vertebrae taken by the DFIS under different loads (0 kg, 5 kg, 10 kg) and different body postures (maximum flexion and extension, maximum left and right bending, and maximum left and right torsion) were captured. Finally, in the Rhino software, the instantaneous motion state of the lumbar spine is reproduced by translation and rotation according to the anatomical structure of the lumbar spine and the previous images. With the help of computer software, a Cartesian coordinate system was placed in the center of each articular surface to measure the kinematics of the articular process and to obtain 6DOF data under different loads (0 kg, 5 kg, 10 kg) in the lumbar facet joints.  RESULTS: In the flexion and extension of the trunk, weight bearing reduced the translational range in the mid-lateral direction. In the L3/4 segment, the lateral translational range of the left and right facet joints gradually decreased with increasing load, and the translational range at 0 kg was significantly greater than that at 10 kg (left side: 0 kg, 0.86° ± 0.57°, 10 kg, 0.24° ± 0.26°, p = 0.01; right side: 0 kg, 0.86° ± 0.59°, 10 kg, 0.26° ± 0.27°, p = 0.01). In the L5/S1 segment, the translation range of the LFJ at 0 kg was significantly greater than that at 10 kg (p = 0.02). Other bending and rotation movements were not found to cause differential changes in the 6DOF of the LFJ. In bending, the rotation range was the largest in the L3/4 segment (p < 0.05) and gradually decreased from top to bottom. At the same level, there were significant differences in the translation range of the left and right facets in the anterior posterior and craniocaudal directions (p < 0.05)., Conclusion: Increasing the load has a significant impact on the coupled translational movement of lumbar facet joints. The asymmetric translational movement of the left and right facet joints may be a factor that accelerates the degeneration of facet joints., (© 2022. The Author(s).)

Rationally integrating multi-active sites into one ideal catalyst is an effective approach to accelerate multistep reactions by synergic catalysis. Herein, a universal and facile room temperature impregnation strategy is developed to construct Ru atomically dispersed catalyst (Ru ADC) with Ru-C 5 single atoms and Ru oxide nanoclusters (≈1.5 nm), which can also be extended to prepare Ir, Rh, Pt, Au, and Mo atomically dispersed catalysts (ADCs). It is found that the obtained Ru ADC largely boosts alkali hydrogen evolution by concerted catalysis between single atoms and sub-nanoclusters, which only needs an overpotential of 18 mV at 10 mA cm -2 . Further mechanistic studies reveal that Ru-C 5 single atoms and Ru oxide nanoclusters with Ru-O 4 configuration in one catalyst can synergically boost water molecule capture, water dissociation, and hydrogen release. This study opens up a simple method to synthesize dual-site metal ADCs for synergic catalysis of typical multistep reactions., (© 2021 Wiley-VCH GmbH.)

Competing Interests: Competing interests: The BMJ has judged that there are no disqualifying financial ties to commercial companies. The authors declare the following other interests: none.

Background: Little is known about the population pharmacokinetics (PPK) of daptomycin in kidney transplant patients. The present study established a pharmacokinetic model for daptomycin in kidney transplant patients in China and examinee the important factors affecting the pharmacokinetic parameters of daptomycin., Methods: The study population included 49 kidney transplant patients with 537 daptomycin concentrations. The PPK model of daptomycin was developed using a nonlinear mixed-effects model, a two-compartment structural model, and a mixed residual error model. The stability and predictive ability of the final model were evaluated based on bootstrapping, visual prediction checks and normalized prediction distribution errors., Results: Glomerular filtration rate (GFR) and total body weight significantly affected clearance, and body weight influenced the central volume of distribution. The average clearance of the population was 0.316 L/h, the central volume of distribution was 6.04 L, the intercompartmental clearance was 2.31 L/h, and the peripheral volume of distribution was 2.46 L. Based on the established model and the target of area under curve (AUC 0-24h )/minimum inhibition concentration (MIC) ≥666, we developed a recommended dose regimen for kidney transplant patients according to their renal function and weight. The daily doses were 4.0±0.31, 4.7±0.36, 5.1±0.40, 5.5±0.43, 5.8±0.45, and 6.1±0.48 mg/kg when the GFRs were 15, 30, 45, 60, 75, and 90 ml/min/1.73 m 2 , respectively., Conclusion: This study provides a reference for individualized daptomycin administration in kidney transplant recipients, and it is a valuable resource for improving the treatment effect and reducing the toxic effects of daptomycin., (Copyright © 2021. Published by Elsevier B.V.)

Competing Interests: Conflicts of interest None disclosed.

Governments worldwide have taken unprecedented social distancing and community lockdown measures to halt the COVID-19 epidemic, leaving millions of people restrained in locked-down communities and their mental well-being at risk. This study examines Chinese rural residents' mental health risk under emergency lockdown during the COVID-19 pandemic. It investigates how the environmental, socioeconomic, and behavioral dimensions of community support affect mental health in this emergency context. We also explore whether community support's effectiveness depends on the strictness of lockdown measures implemented and the level of individual perceived COVID-19 infection risk. We collect self-reported mental health risk, community support, and demographics information through a cross-sectional survey of 3892 Chinese rural residents living in small towns and villages. Ordinary least square regressions are employed to estimate the psychological effects of community support. The results suggest that the COVID-19 epidemic and lockdown policies negatively affect psychological well-being, especially for rural females. The capacity for community production has the largest impact on reducing mental health risks, followed by the stability of basic medical services, community cohesion, housing condition, the stability of communications and transportation supply, and the eco-environment. The effectiveness of different community support dimensions depends on the level of lockdown policy implemented and the levels of one's perceived risk of COVID-19 infection. Our study stresses the psychological significance of a healthy living environment, resilient infrastructure and public service system, and community production capacity during the lockdown in rural towns and villages., Competing Interests: We declare no competing interests., (© 2021 Elsevier Ltd. All rights reserved.)

Background: Pituitary tumors are among the most common intracranial tumors. Surgical resection is the most effective treatment for patients with pituitary tumors. Microscopic transsphenoidal surgery has become the first-choice surgical approach to treating this malignancy, although it has certain limitations. Neuroendoscopy has also been widely used for pituitary tumor surgery in recent years. This study aimed to compare the efficacy and safety of these two surgical options for the treatment of pituitary tumors., Methods: We conducted a literature search of the PubMed, Embase, Cochrane Controlled Center Register of Controlled Trials (CENTRAL), Web of Science database, Google Scholar, and Baidu Scholar. Relevant articles published up to September 25, 2020 were retrieved and then meta-analyzed using RevMan software 5.1., Results: A total of 29 case-control studies involving 7,774 patients were included in the meta-analysis. There was no significant difference in gross tumor removal (GTR) (RR =1.11, 95% CI: 0.97-1.26, P=0.12) or hormone excess secretion (HES) remission (RR =1.08, 95% CI: 0.97-1.21, P=0.16) between the two groups. Endoscopic transsphenoidal surgery was associated with a lower incidence of diabetes insipidus (DI) than was microscopic transsphenoidal surgery (RR =0.76, 95% CI: 0.60-0.97, P=0.03)., Conclusions: Endoscopic transsphenoidal surgery does not significantly improve GTR or HES remission, but it can reduce the incidence of DI without increasing the rates of other complications., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/gs-20-851). The authors have no conflicts of interest to declare., (2020 Gland Surgery. All rights reserved.)

Identification of an active center of catalysts under realistic working conditions of oxygen reduction reaction (ORR) still remains a great challenge and unclear. Herein, we synthesize the Cu single atom embedded on nitrogen-doped graphene-like matrix electrocatalyst (abbreviated as SA-Cu/NG). The results show that SA-Cu/NG possesses a higher ORR capability than 20% Pt/C at alkaline solution while the inferior activity to 20% Pt/C at acidic medium. Based on the experiment and simulation calculation, we identify the atomic structure of Cu-N 2 C 2 in SA-Cu/NG and for the first time unravels that the oxygen-reconstituted Cu-N 2 C 2 -O structure is really the active species of alkaline ORR, while the oxygen reconstitution does not happen at acidic medium. The finding of oxygen-reconstituted active species of SA-Cu/NG at alkaline media successfully unveils the bottleneck puzzle of why the performance of ORR catalysts at alkaline solution is better than that at acidic media, which provides new physical insight into the development of new ORR catalysts., Competing Interests: The authors declare that they have no conflict of interest., (Copyright © 2020 Liu Yang et al.)

Background: To observe cumulative morbidity of postoperative inguinal hernia (PIH) and identify risk factors associated with its development in patients who underwent retropubic radical prostatectomy (RRP), laparoscopic prostatectomy (LRP) or robotic assisted laparoscopic prostatectomy (RALP) operation., Methods: From June 2009 to September 2016, 756 patients diagnosed with localized prostate cancer who had undergone RRP, LRP or RALP in our center were included in this study. Patients with PIH were retrospectively investigated in such factors as age, BMI, previous abdominal operations, diabetes mellitus history, hypertension history, prostate volume, previous hernia, operative methods, operative approach, preoperative Gleason, clinical T-stage, PLND situation, operative time, and estimated blood loss. Univariate and multivariate cox hazard regressions analysis were utilized to identify risk factors predisposing to PIH., Results: A total of 53 of 751(7.1%) patients developed PIH at a median follow-up period of 43 months. PIH rate in RRP was significantly higher compared to LRP and RALP group (RRP: 15.3%, LRP: 6.7%, RALP:1.9%, P = 0.038). Right side (69.8%) and indirect (88.8%) PIH were dominant type in hernia group. Univariate and multivariate cox hazard regressions analysis indicated that age and RRP approach were identified to be implicated to PIH [adjusted hazard ratio7.39(1.18-46.39), 2.93(95% CI 1.47-5.84)]., Conclusions: RRP technique and older age, especially patients over 80 years, are associated with higher incidence for PIH development. Appropriate prophylaxis during the operation should be evaluated for those in high-risk.

Rational design of low-cost, highly efficient, and stable electrocatalysts for the hydrogen evolution reaction (HER) has attracted wide attention. Herein, 3D RuCu nanocrystals (NCs) are successfully synthesized by a facile wet chemistry method, in which amorphous RuCu nanosheets are directly grown on crystalline Cu nanotubes (NTs). Importantly, the obtained 3D RuCu NCs only need 18 and 73 mV to deliver the current density of 10 mA cm -2 for HER in alkaline and neutral media, respectively. Density functional theory calculations and experiments reveal that the Ru sites on the surface of amorphous nanosheets are the highly active centers for HER. Moreover, this catalyst can expose more surface area for water splitting compared to pure nanosheets because the unique 3D structure can effectively prevent the aggregation of nanosheets. Meanwhile, the interface between amorphous nanosheets and crystalline NTs is essential to boost the HER performance because the amorphous phase with many unsaturated bonds can facilitate adsorption of reactants and crystalline Cu with superior conductivity can promote the transfer of electrons. This work provides a facile method to prepare an original 3D Ru-based electrocatalyst with highly active HER performance in wide pH values., (© 2020 Wiley-VCH GmbH.)