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The present study has focused on the mainstream integration of polyhydroxyalkanoate (PHA) production with industrial wastewater treatment by exploiting three different technologies all operating in sequencing batch reactors (SBR): conventional activated sludge (AS-SBR), membrane bioreactor (AS-MBR) and aerobic granular sludge (AGS). A full aerobic feast/famine strategy was adopted to obtain enrichment of biomass with PHA-storing bacteria. All the systems were operated at different organic loading (OLR) rate equal to 1-2-3 kgCOD/m 3 ∙d in three respective experimental periods. The AS-MBR showed the better and stable carbon removal performance, whereas the effluent quality of the AS-SBR and AGS deteriorated at high OLR. Biomass enrichment with PHA-storing bacteria was successfully obtained in all the systems. The AS-MBR improved the PHA productivity with increasing OLR (max 35% w/w), whereas the AS-SBR reduced the PHA content (max 20% w/w) above an OLR threshold of 2 kgCOD/m 3 ∙d. In contrast, in the AGS the increase of OLR resulted in a significant decrease in PHA productivity (max 14% w/w) and a concomitant increase of extracellular polymers (EPS) production (max 75% w/w). Results demonstrated that organic carbon was mainly driven towards the intracellular storage pathway in the AS-SBR (max yield 51%) and MBR (max yield 61%), whereas additional stressors in AGS (e.g., hydraulic selection pressure, shear forces) induced bacteria to channel the COD into extracellular storage compounds (max yield 50%) necessary to maintain the granule's structure. The results of the present study indicated that full-aerobic feast/famine strategy was more suitable for flocculent sludge-based technologies, although biofilm-like systems could open new scenarios for other biopolymers recovery (e.g., EPS). Moreover, the AS-MBR resulted the most suitable technology for the integration of PHA production in a mainstream industrial wastewater treatment plant, considering the greater process stability and the potential reclamation of the treated wastewater., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The present study investigated the combined production of reclaimed water for reuse purposes and polyhydroxyalkanoates (PHA) from an agro-food industrial wastewater. A pilot plant implementing a two-stage process for PHA production was studied. It consisted of a mainstream sequencing batch membrane bioreactor (SBMBR) in which selection of PHA-accumulating organisms and wastewater treatment were carried out in, and a side-stream fed-batch reactor (FBR) where the excess sludge from the SBMBR was used for PHA accumulation. The performance of the SBMBR was compared with that of a conventional sequencing batch reactor (SBR) treating the same wastewater under different food to microorganisms' ratios (F/M) ranging between 0.125 and 0.650 kgCOD kgTSS -3 d -1 . The SBMBR enabled to obtain very high-quality effluent in compliance with the relevant national (Italy) and European regulations (Italian DM 185/03 and EU, 2020/741) in the field of wastewater reclamation, whereas the performances in the SBR collapsed at F/M higher than 0.50 kgCOD kgTSS -1 d -1 . A maximum intracellular storage of 45% (w/w) and a production yield of 0.63 gPHA L -1 h -1 were achieved when the SBMBR system was operated with a F/M ratio close to 0.50 kgCOD kgTSS -1 d -1 . This resulted approximately 35% higher than those observed in the SBR, since the ultrafiltration membrane avoided the washout of dispersed and filamentous bacteria capable of storing PHA. Furthermore, while maximizing PHA productivity in conventional SBR systems led to process dysfunctions, in the SBMBR system it helped mitigate these issues by reducing membrane fouling behaviour. The results of this study supported the possibility to achieve combined recovery of reclaimed water and high-value added bioproducts using membrane technology, leading the way for agro-food industrial wastewater valorization in the frame of a circular economy model., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Green Walls represent a sustainable solution to mitigate the effects due to climate change and urbanization. However, although they have been widely investigated in different fields of science, studies on the potential of these systems to manage urban stormwater are still few. Moreover, even if these systems provide multiple benefits, as other nature-based solutions, they leach nutrients due to growing media, decomposed vegetation, and the possibility of fertilizer use. In this regard, several studies have evaluated the nutrient concentrations in the runoff from green roofs, while studies that have analyzed the nutrient-leaching behavior of green walls are still limited. To bridge these scientific gaps, this study presents experimental findings on the hydrological efficiency and nutrient-leaching behavior of an innovative modular living wall system. Some rainfall-runoff tests were carried out to assess the hydrological response of a new green wall system in retaining stormwater. To evaluate the concentration of the nutrients, the collected outflow was analyzed by spectrophotometer UV-visible. The findings show that the developed green wall panel presents good retention capacity by considering different simulated rainfalls and varying the initial soil moisture conditions. The results in terms of nutrient concentrations highlight that the vegetation life cycle and the fertilizer uses affect the quality of the water released from the green wall panel. The concentration of the analyzed nutrients is influenced by the simulated rainfall's hydrological characteristics and the days between the planting phase and the test. However, the overall results show that the concentrations of each analyzed nutrient are low, except after the fertilizer use, highlighting that the choice of vegetation that does not need external nutrients should be preferred during the design of a green wall., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

In the present research, insights about the mechanisms of excess sludge minimization occurring in an oxic-settling-anaerobic (OSA) were provided. The investigation involved two systems operating in parallel. In particular, a conventional activated sludge (CAS) system as control and a system implementing the OSA process both having a pre-denitrification scheme were considered. Five periods (P1-P5) were studied, during which several operating conditions and configurations were tested. Specifically, the hydraulic retention time (HRT) in the anaerobic reactor of the OSA system (P1 8 h, P2-P3 12 h, P4 8 h, P5 12 h) and the return sludge from the anaerobic to the anoxic (scheme A) (P1-P2) or aerobic (scheme B) mainstream reactors (P3-P5) were investigated. The results highlighted that the excess sludge production in the OSA was lower in all the configurations (12-41%). In more detail, the observed yield (Y obs ) was reduced from 0.50-0.89 gTSS gCOD -1 (control) to 0.22 -0.34 gTSS gCOD -1 in the OSA process. The highest excess sludge reduction (40%) was achieved when the OSA was operated according to scheme B and HRT of 12 h in the anaerobic reactor (P3). Generally, scheme A enabled the establishment of cell lysis and extracellular polymeric substances (EPS) destructuration, leading to a worsening of process performances when high anaerobic HRT (>8 h) was imposed. In contrast, scheme B enabled the establishment of maintenance metabolism in addition to the uncoupling metabolism, while cell lysis and EPS destruction were minimized. This allowed obtaining higher sludge reduction yield without compromising the effluent quality., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Citrus wastewaters (CWWs) are by-products of the citrus fruit transformation process. Currently, more than 700 million of m³ of CWWs per year are produced worldwide. Until nowadays, the management of CWWs is based on a take-make-use-dispose model. Indeed, after being produced within a citrus processing industry, CWWs are subjected to treatment and then discharged into the environment. Now, the European Union is pushing towards a take-make-use-reuse management model, which suggests to provide for the minimization of residual pollutants simultaneously with their exploitation through a biorefinery concept. Indeed, the recovery of energy nutrients and other value-added products held by CWWs may promote environmental sustainability and close the nutrient cycles in line with the circular bio-economy perspective. Unfortunately, knowledge about the benefits and disadvantages of available technologies for the management and valorisation of CWWs are very fragmentary, thus not providing to the scientific community and stakeholders an appropriate approach. Moreover, available studies focus on a specific treatment/valorisation pathway of CWWs and an overall vision is still missing. This review aims to provide an integrated approach for the sustainable management of CWWs to be proposed to company managers and other stakeholders within the legislative boundaries and in line with the circular bio-economy perspective. To this aim, firstly, a concise analysis of citrus wastewater characteristics and the main current regulations on CWWs are reported and discussed. Then, the main technologies with a general comparison of their pros and cons, and alternative pathways for CWWs utilization are presented and discussed. Finally, a focus was paid to the economic feasibility of the solutions proposed to date relating to the recovery of the CWWs for the production of both value-added compounds and agricultural reuse. Based on literature analysis an integrated approach for a sustainable CWWs management is proposed. Such an approach suggests that after chemicals recovery by biorefinery, wastewaters should be directly used for crop irrigation if allowed by regulations or addressed to treatment plant. The latter way should be preferred when CWWs cannot be directly applied to soil due to lack of concomitance between CWWs production and crop needs. In such a way, treated wastewater should be reused after tertiary treatments for crop irrigation, whereas produced sludges should be undergone to dewatering treatment before being reused as organic amendment to improve soil fertility. Finally, this review invite European institutions and each Member State to promote common and specific legislations to overcome the fragmentation of the regulatory framework regarding CWWs reuse., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Fouling is considered one of the main drawbacks of membrane bioreactor (MBR) technology. Among the main fouling agents, extracellular polymeric substances (EPS) are considered one of the most impactful since they cause the decrease of sludge filterability and decline of membrane flux in the long term. The present study investigated a biological strategy to reduce the membrane-fouling tendency in MBR systems. This consisted of seeding the reactor with activated sludge enriched in microorganisms with polyhydroxyalkanoate (PHA) storage ability and by imposing proper operating conditions to drive the carbon toward intracellular (PHA) rather than extracellular (EPS) accumulation. For that purpose, an MBR lab-scale plant was operated for 175 days, divided into four periods (1-4) according to different food to microorganisms' ratios (F/M) (0.80 kg COD kg TSS -1 d -1 (Period 1), 0.13 kg COD kg TSS -1 d -1 (Period 2), 0.28 kg COD kg TSS -1 d -1 (Period 3), and 0.38 kg COD kg TSS -1 d -1 (Period 4)). The application of the feast/famine strategy favored the accumulation of intracellular polymers by bacteria. The increase of the PHA accumulation inside the cells corresponded to the decrease of EPS and an F/M of 0.40-0.50 kg COD kg TSS -1 d -1 was found as optimum to maximize the PHA production, while minimizing EPS. The lowest EPS content in the sludge (18% of total suspended solids) that corresponded to the maximum content of PHA (9.3%) was found in Period 4 and determined significant mitigation of the fouling rate, whose value was close to 0.10 × 10 11 m -1 h -1 . Thus, by imposing proper operating conditions, it was possible to drive the organic matter toward PHA accumulation. Moreover, a lower EPS content corresponded to a decrease in the irreversible fouling mechanism, which would imply a lower frequency of the extraordinary cleaning operations. This study highlighted the possibility of obtaining a double benefit by applying an MBR system in the frame of wastewater valorization: minimizing the fouling tendency of the membrane and recovery precursors of bioplastics from wastewater in line with the circular economy model.

In this study, the presence of microplastics in the sludge of three wastewater treatment plants (WWTPs) was examined. The investigated WWTPs operated based on a conventional activated sludge (CAS) process, with (W1) or without (W2) primary clarification, and a membrane bioreactor process (MBR) (W3). The microplastics (MPs) concentration in the samples of W3 was approximately 81.1 ± 4.2 × 10 3 particles/kg dry sludge, whereas MPs concentrations in W1 and W2 were 46.0 ± 14.8 × 10 3 particles/kg dry sludge and 36.0 ± 5.2 × 10 3 particles/kg dry sludge, respectively. Moreover, MPs mainly consisted of fragments (66-68%) in the CAS plants, whereas the fractions of MPs shapes in the MBR sludge were more evenly distributed, although fiber (47%) was the most abundant fraction. Furthermore, samples from the MBR showed a greater diversity in MPs composition. Indeed, all the main polyesters (i.e., textile fibers and polyethylene terephthalate), polyolefins (i.e., polyethylene and polypropylene) and rubber (i.e., polybutadiene) were observed, whereas only polybutadiene, cellulose acetate and polyester were detected in the CAS plants. These findings confirmed that MPs from wastewater are transferred and concentrated in the waste sludge. This is a critical finding since sludge disposal could become a new pathway for microplastic release into the environment and because MPs might affect the fouling behavior of the membrane.

Contaminated marine sediments represent a critical threat towards human health and ecosystems, since they constitute a potential reservoir of toxic compounds release. In the present study, a bioslurry reactor was studied for the treatment of real marine sediments contaminated by petroleum hydrocarbons. The experimental campaign was divided in two periods: in the first period, microcosm trials were carried out to achieve useful indicators for biological hydrocarbon removal from sediments. The microcosm trials highlighted that the inoculum of halotolerant allochthonous bacteria provided the highest performance followed by autochthonous biomass. Based on the achieved results, in the second experimental period a bioslurry reactor was started up, based on a semisolid stirred tank reactor (STR) operated in batch mode. The process performances have been evaluated in terms of total petroleum hydrocarbon (TPH) removal, coupled with the characterization of microbial community through a Next Generation Sequencing (NGS) and phytotoxicity tests through the Germination Index (GI) with Lepidium Sativum seeds. The achieved results showed good hydrocarbons removal, equal to 40%, with a maximum removal rate of 220 mg TPH kg -1 d -1 , but highlighting that high contaminant concentrations might affect negatively the overall removal performance. In general, the observed results were encouraging towards the feasibility of biological treatment of marine sediments contaminated by hydrocarbons. The microbiological analysis allowed the identification of taxa most involved in the degradation of TPH, highlighting after the treatment a shift in the microbial community from that of the raw sediment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The aim of this study was to evaluate the effect of the inoculum to substrate ratio (ISR) and the mixture ratio between organic fraction of municipal solid waste (OFMSW) and sewage sludge (SS) on the methane production potential achievable from anaerobic co-digestion (AcoD). Biochemical Methane Potential (BMP) assays at mesophilic temperature were used to determine the best AcoD configuration for maximizing methane yield and production rate, as well as to address possible synergistic effects. The maximum methane yield was observed at ISR of 1 and 60% OFMSW: 40% SS as co-digestion mixture, whereas the highest methane production rate was achieved at ISR of 2 with the same mixture ratio (207 mL/gVS/d). Synergistic effects were highlighted in the mixtures having OFMSW below 60%, determining an increase of approximately 40% in methane production than the OFMSW and SS digestion as a sole substrate. The experimental data demonstrated that co-digestion of OFMSW and SS resulted in an increase in the productivity of methane than anaerobic digestion using the sole substrates, producing higher yields or production rates while depending on the ISR and the mixture ratio.

The integration of one anaerobic reactor in the mainstream (AMSR) of a pre-denitritication-MBR was evaluated with the aim to achieve simultaneous sludge minimization and phosphorous removal. The excess sludge production was reduced by 64% when the AMSR was operated under 8 h of hydraulic retention time (HRT). The highest nutrients removal performances referred to organic carbon (98%), nitrogen (90%) and phosphorous (97%) were obtained under 8 h of HRT. In contrast, prolonged anaerobic-endogenous conditions were found to be detrimental for all nutrients removal performances. Similarly, the lowest membrane fouling tendency (FR = 0.65∙10 11  m -1  d -1 ) was achieved under 8 h of HRT, whereas it significantly increased under higher HRT. The highest polyphosphate accumulating organisms kinetics were achieved under HRT of 8 h, showing very high exogenous P-release (46.67 mgPO 4 -P gVSS -1 h -1 ) and P-uptake rates (48.6 mgPO 4 -P gVSS -1 h -1 ), as well as a not negligible P-release rate under endogenous conditions at low COD/P ratio (≈1)., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The biodegradability and treatability of a young (3 years old) municipal landfill leachate was evaluated by means of chemical oxygen demand (COD) fractionation tests, based on respirometric techniques. The tests were performed using two different biomasses: one cultivated from the raw leachate (autochthonous biomass) and the other collected from a conventional municipal wastewater treatment plant after its acclimation to leachate (allochthonous biomass). The long term performances of the two biomasses were also studied. The results demonstrated that the amount of biodegradable COD in the leachate was strictly dependent on the biomass that was used to perform the fractionation tests. Using the autochthonous biomass, the amount of biodegradable organic substrate resulted in approximately 75% of the total COD, whereas it was close to 40% in the case of the allochthonous biomass, indicating the capacity of the autochthonous biomass to degrade a higher amount of organic compounds present in the leachate. The autochthonous biomass was characterized by higher biological activity and heterotrophic active fraction (14% vs 7%), whereas the activity of the allochthonous biomass was significantly affected by inhibitory compounds in the leachate, resulting in a lower respiration rate (SOUR = 13 mg O 2 gVSS -1  h -1 vs 37 mg O 2 gVSS -1  h -1 ). The long-term performance of the autochthonous and allochthonous biomasses indicated that the former was more suitable for the treatment of raw landfill leachate, ensuring higher removal performance towards the organic pollutants., (Copyright © 2019 Elsevier B.V. All rights reserved.)

This study investigated the chance to couple the conventional Oxic Settling Anaerobic (OSA) process with a thermic treatment at moderate temperature (35 °C). The maximum excess sludge reduction rate (80%) was achieved when the plant was operated under 3 h of hydraulic retention time (HRT). Compared with the conventional OSA system, the thermic treatment enabled a further improvement in excess sludge minimization of 35%. The observed yield coefficient decreased from 0.25 gTSS gCOD -1 to 0.10 gTSS gCOD -1 when the temperature in the anaerobic reactor was increased to 35 °C, despite the lower HRT (3 h vs 6 h). Moreover, the thermic treatment enabled the decrease of filamentous bacteria, thereby improving the sludge settling properties. The thermic treatment enhanced the destruction of extracellular polymeric substances and the increase of endogenous decay rate (from 0.64 d -1 to 1.16 d -1 ) that reduced the biomass active fraction (from 22% to 4%)., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

A University of Cape Town Integrated Fixed-Film Activated Sludge Membrane Bioreactor (UCT-IFAS-MBR) pilot plant was operated at different values of the sludge retention time (SRT). Three SRTs were investigated at different durations: indefinitely, 30 and 15 days. The organic carbon, nitrogen and phosphorus removal, kinetic/stoichiometric parameters, membrane fouling tendency and sludge filtration properties were assessed. The findings showed that by decreasing the SRT, the pilot plant could maintain excellent carbon removal efficiencies throughout the experiments. In contrast, the biological carbon removal showed a slight nitrification and was slightly affected by the decrease of the SRT, showing high performance (approximately 91%, on average). Thus, the biofilm might have helped sustain the nitrification throughout the experiments. The average phosphorus removal performance increased slightly with a decrease in SRT, achieving the maximum efficiency (61.5%) at a SRT of 15 days. After a 30-day SRT, an increase in resistance due to pore blocking and a general worsening of the membrane filtration properties occurred.

Bio-trenches are a sustainable option for treating nitrate contamination in groundwater. However, a possible side effect of this technology is the production of nitrous oxide, a greenhouse gas that can be found both dissolved in the liquid effluent as well as emitted as off gas. The aim of this study was to analyze NO 3 - removal and N 2 O production in lab-scale column trials. The column contained olive nut as organic carbon media. The experimental study was divided into three phases (I, II and III) each characterized by different inlet NO 3 - concentrations (30, 50, 75mgNO 3 -NL -1 respectively). Sampling ports deployed along the length of the column allowed to observe the denitrification process as well as the formation and consumption of intermediate products, such as nitrite (NO 2 - ) and nitrous oxide (N 2 O). In particular, it was observed that N 2 O production represent only a small fraction of removed NO 3 - during Phase I and II, both for dissolved (0.007%) and emitted (0.003%) phase, and it was recorded a high denitrification efficiency, over 99%. Nevertheless, significantly higher values were recorded for Phase 3 concerning emitted phase (0.018%). This fact is due to increased inlet concentration which resulted in a carbon limitation and in a consequent decrease in denitrification efficiency (76%)., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Excess sludge minimization was studied in a MBR with pre-denitrification scheme. Sludge minimization, nitrogen removal performance and membrane fouling tendency were investigated in two configurations, characterized by a different position of the sludge retention reactor (SRR). In particular, the SRR was placed: i) in the return activated sludge line (Anaerobic Side-Stream Reactor - ASSR configuration) and ii) in the mainstream between the anoxic and aerobic reactor (Anaerobic Main-Stream Reactor - AMSR configuration). The achieved results demonstrated that the ASSR enabled a higher excess sludge reduction (74% vs 32%), while achieving lower biological nitrogen removal (BNR) (TN = 63% vs 78%) and membrane fouling tendency (FR = 2.1 · 10 12  m -1  d -1 vs 4.0 · 10 11  m -1  d -1 ) than the AMSR. It was found that metabolism uncoupling, destruction of EPS and endogenous decay simultaneously occurred in the ASSR. Conversely, selective enrichment of bacteria population with low biomass yield was found the main mechanism affecting sludge minimization in the AMSR., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

In the present paper, the feasibility of citrus wastewater treatment with aerobic granular sludge sequencing batch reactors (AGSBR) was investigated. Two AGSBRs (named R1 and R2, respectively) were operated for 90 days under different organic loading rates (OLR) and pH in two experimental periods. The OLR ranged approximately between 3.0 kg TCOD m -3 d -1 and 7 kg TCOD m -3 d -1 during Period I, whereas between 7 kg TCOD m -3 d -1 and 15 kg TCOD m -3 d -1 during Period II. pH was maintained at 7.0 and 5.5 in R1 and R2, respectively. The results revealed that under high OLR and unbalanced feast/famine regime (Period I), the development of fast-growing microorganisms (fungi and filamentous bacteria) was favoured in both reactors, resulting in granular sludge instability. An extended famine phase and a proper balancing between feast and famine periods (Period II) were favourable for the development of bacteria with low growth rates (0.05 d -1 ) thus enhancing the granules stability. To the benefit of granular sludge stability and effluent quality, the length of the feast period should not exceed 25% of cycle length. Moreover, under OLR lower than 7 kg TCOD m -3 d -1 the removal efficiency of total chemical oxygen demand (TCOD) was approximately 90% in R1 and R2 and no side effects on the organic carbon removal performance related to the pH were observed. In contrast, at higher OLR a significant decrease in the removal efficiency (from 90% to less than 75%) was observed in R2. Results revealed also that under low pH, hydrolysis of proteins occurred and a decrease in the biological kinetic rates proportionally to the applied OLR was observed., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

In this study the occurrence and the behavior of illicit drugs and their metabolites have been investigated for two wastewater treatment plants (WWTPs) (namely, WWTP-1 and WWTP-2) located in Sicily (island of Italy). Samples were analyzed for methamphetamine, cocaine (COC), 3,4-methylenedioxymethamphetamine (MDMA), methadone (METH), 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), 3,4-methylenedioxy amphetamine (MDA); 3,4-methylenedioxy ethylamphetamine (MDEA), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) and Benzoylecgonine (BEG). The BEG, COC, MOR and THC-COOH were found at the highest concentration in both WWTPs. The Wastewater-based epidemiology calculation for BEG, COC, cannabinoids and THC-COOH was performed. On average, for both plants, population consumes 1.6 and 23.4 dose 1000 inh -1 day -1 of cocaine and cannabis, respectively. For WWTP-1 negative removals of illicit drugs were observed. For WWTP-2 the following average removal efficiencies were obtained: BEG (77.85%), COC (92.34%), CODEINE (64.75%), MOR (90.16%) and THC-COOH (68.64%)., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Autochthonous halophilic biomass was cultivated in a sequencing batch reactor (SBR) aimed at analyzing the potential use of autochthonous halophilic activated sludge in treating saline industrial wastewater. Despite the high salt concentration (30 g NaCl L -1 ), biological oxygen demand (BOD) and total suspended solids (TSS), removal efficiencies were higher than 90%. More than 95% of the nitrogen was removed via a shortcut nitrification-denitrification process. Both the autotrophic and heterotrophic biomass samples exhibited high biological activity. The use of autochthonous halophilic biomass led to high-quality effluent and helped to manage the issues related to nitrogen removal in saline wastewater treatment., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Introduction: A wastewater-based epidemiology approach was performed to estimate the drug consumption in Palermo city, the fifth largest city of Italy with a population of 671 696 inhabitants, and to investigate the monthly variability of drug loads in wastewater from different areas of the city. A seven-months detection campaign was conducted at the two wastewater treatment plants of the city., Methods: Following a pre-treatment, 32 samples of wastewater were analyzed by liquid chromatography-tandem mass spectrometry., Results: We estimated a mean cocaine use in Palermo of 0.19 g/day/1000 people, corresponding to 1.90 doses/1000 people and cannabinoids use of 2.85 g/day/1000 people, corresponding to 35.62 doses/1000 people. Amphetamines residues in wastewater were always recovered in concentrations lower than the limit of quantification., Conclusion: Our findings showed that drugs consumption in Palermo is in line with those of other Italian cities and that no significant differences on prevalence on cocaine and cannabinoids consumption were recorded in the different months of the survey, except for the summer period in a wastewater treatment plant of the city.

The modification of the physical properties of aerobic granular sludge treating fish-canning wastewater is discussed in this paper. The structure and composition of the Extracellular Polymeric Substances (EPSs) were analyzed at different salinity levels and related to granules stability. Results outlined that the total EPSs content increased with salinity, despite the EPSs increment was not proportional to the salt concentration. Moreover, the EPSs structure was significantly modified by salinity, leading to a gradual increase of the not-bound EPSs fraction, which was close to the 50% of the total EPSs content at 75gNaClL -1 . The increasing salt concentration modified also the EPSs composition, causing the gradual reduction of protein content resulting in a decrease of granule hydrophobicity. The results pointed out that the granules stability significantly reduced above 50gNaClL -1 , suggesting the existence of a salinity threshold above which granules stability is compromised., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

This work aims to investigate the stability of aerobic granular sludge in the long term, focusing on the clogging of the granular sludge porosity exerted by the extracellular polymeric substances (EPSs). The effects of different cycle lengths (short and long-term cycle) on the granular sludge stability were investigated. Results obtained outlined that during the short duration cycle, the formation and breakage of the aerobic granules were continuously observed. During this period, the excess of EPS production contributed to the clogging of the granules porosity, causing their breakage in the long run. During the long-duration cycle, the extended famine period entailed a greater EPSs consumption by bacteria, thus limiting the clogging of the porosity, and allowed obtaining stable aerobic granules. Reported results demonstrated that an excess in EPSs content could be detrimental to the stability of aerobic granular sludge in the long-term., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

In this work, a sequential batch membrane bioreactor pilot plant is investigated to analyze the effect of a gradual increase in salinity on carbon and nutrient removal, membrane fouling and biomass kinetic parameters. The salinity was increased by 2gNaClL(-1) per week up to 10gNaClL(-1). The total COD removal efficiency was quite high (93%) throughout the experiment. A gradual biomass acclimation to the salinity level was observed during the experiment, highlighting the good recovery capabilities of the system. Nitrification was also influenced by the increase in salinity, with a slight decrease in nitrification efficiency (the lowest value was obtained at 10gNaClL(-1) due to lower nitrifier activity). Irreversible cake deposition was the predominant fouling mechanism observed during the experiment. Respirometric tests exhibited a stress effect due to salinity, with a reduction in the respiration rates observed (from 8.85mgO2L(-1)h(-1) to 4mgO2L(-1)h(-1))., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Fish processing industries produce wastewater containing high amounts of salt, organic matter and nitrogen. Biological treatment of such wastewaters could be problematic due to inhibitory effects exerted by high salinity levels. In detail, high salt concentrations lead to the accumulation of nitrite due to the inhibition of nitrite-oxidizing bacteria. The feasibility of performing simultaneous nitritation and denitritation in the treatment of fish canning wastewater by aerobic granular sludge was evaluated, and simultaneous nitritation-denitritation was successfully sustained at salinities up to 50 gNaCl L(-1), with a yield of over 90%. The total nitrogen concentration in the effluent was less than 10 mg L(-1) at salinities up to 50 gNaCl L(-1). Nitritation collapsed above 50 gNaCl L(-1), and then, the only nitrogen removal mechanism was represented by heterotrophic synthesis. In contrast, organic matter removal was not affected by salinity but was instead affected by the organic loading rate (OLR). Both COD and BOD removal efficiencies were over 90%. The COD fractionation analysis indicated that aerobic granules were able to remove more than 95% of the particulate organic matter. Finally, results obtained in this work noted that aerobic granular sludge had an excellent ability to adapt under adverse environmental conditions., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

In order to prevent hydrocarbon discharge at sea from ships, the International Maritime Organization (IMO) enacted the MARPOL 73/78 convention in which any oil and oil residue discharged in wastewater streams must contain less than 5 ppm hydrocarbons. Effective treatment of this petroleum-contaminated water is essential prior to its release into the environment, in order to prevent pollution problem for marine ecosystems as well as for human health. Therefore, two bench scale membrane bioreactors (MBRs) were investigated for hydrocarbon biodegradation. The two plants were initially fed with synthetic wastewater characterised by an increasing salinity, in order to enhance biomass acclimation to salinity. Subsequently, they were fed with a mixture of synthetic wastewater and real shipboard slops (with an increasing slops percentage up to 50% by volume). The results indicated a satisfactory biomass acclimation level in both plants with regards to salinity, providing significant removal efficiencies. The real slops exerted an inhibitory effect on the biomass, partially due to hydrocarbons as well as to other concomitant influences from other compounds contained in the real slops difficult to evaluate a priori. Nevertheless, a slight adaptation of the biomass to the new conditions was observed, with increasing removal efficiencies, despite the significant slops percentage., (Copyright © 2015 Elsevier B.V. All rights reserved.)

The effect of a sharp variation of C/N ratio in a moving bed membrane bioreactor (MB-MBR) pilot plant treating high strength wastewater has been investigated. The experimental campaign was divided into two periods, each characterized by a different C/N ratio (namely, 2.5 and 15, Period 1 and Period 2, respectively). The MB-MBR system was analyzed in terms of organic carbon removal, nitrification efficiency, biokinetic activity and fouling behavior. The results showed that the nitrification process was severely affected by lower C/N value and by high concentration of ammonia. It was noticed an extensive stress effect on the autotrophic bacteria. Furthermore, it was observed an increase of the resistance related to particle deposition into membrane pores, likely due to a worsening of the cake layer features, with a reduction of the "pre-filter" effect, also related to the increase of the total Extracellular Polymeric Substances production with the C/N ratio., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

Three Sicilian wastewater treatment plants were monitored to assess the occurrence and the behaviour of radionuclides. Two sampling campaigns (screening and long-term) were carried out during which liquid and solid samples have been analysed. It was found that ¹³¹I mostly occurred in the samples analysed during the screening campaign (43% of the analysed samples contained ¹³¹I). High ¹³¹I specific activity was found in the mixed liquor, recycled sludge and dehydrated sludge samples. This finding was mainly due to the tendency of (131)I to be associated with solid particles. During the long-term sampling campaign an influence of the sludge retention time (SRT) on the ¹³¹I behaviour was found. Increasing the SRT and consequently decreasing the fraction of active organic biomass inside the system, the specific activity of ¹³¹I decreases.

Two pilot plant systems were investigated for the treatment of wastewater subject to a gradual increase of salinity. In particular, a membrane bioreactor (MBR) and a moving bed biofilm membrane bioreactor (MB-MBR) were analyzed. Carbon and ammonium removal, kinetic constants and membranes fouling rates have been assessed. Both plants showed very high efficiency in terms of carbon and ammonium removal and the gradual salinity increase led to a good acclimation of the biomass, as confirmed by the respirometric tests. Significant biofilm detachments from carriers were experienced, which contributed to increase the irreversible superficial cake deposition. However, this aspect prevented the pore fouling tendency in the membrane module of MB-MBR system. On the contrary, the MBR pilot, even showing a lower irreversible cake deposition, was characterized by a higher pore fouling tendency., (Copyright © 2014 Elsevier Ltd. All rights reserved.)

Membrane bioreactors produce high quality effluents that could be suitable for reuse. However, when treating high strength wastewater subject to a salinity increase, a modification of biomass characteristics may occur. This circumstance is of importance, since it can have a significant impact in terms of biokinetic as well as fouling behaviour. The aim of the study was to evaluate the performance of a pilot plant MBR, in terms of biomass activity and membrane fouling, fed with high strength synthetic wastewater, when subject to a salinity increase. With normal salinity, the pilot plant showed high removal efficiencies and high biomass respiratory activity. On the other hand, the salinity increase significantly affected the removal efficiency as well as the SMP production. Furthermore, the salinity increase showed a strong effect on biomass activity, reducing in particular the respiration rates of autotrophic populations., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

Sanitary landfills for Municipal Solid Waste (MSW) disposal have been identified as one of the most important anthropogenic sources of methane (CH4) emissions; in order to minimize its negative effects on the environment, landfill gas (LFG) recovery is a suitable tool to control CH4 emissions from a landfill site; further, the measurement of CH4 emissions can represent a good way to evaluate the effectiveness of LFG recovering systems. In general, LFG will escape through any faults in the landfill capping or in the LFG collection system. Indeed, some areas of the capping can be more permeable than others (e.g. portions of a side slope), especially when considering a temporarily capped zone (covered area that is not expected to receive any further waste for a period of at least 3 months, but for engineering reasons does not have a permanent cap yet). These areas, which are characterized by abnormal emissions, are usually defined as "features": in particular, a feature is a small, discrete area or an installation where CH4 emissions significantly differ from the surrounding zones. In the present study, the influence that specific features have on CH4 emissions has been investigated, based on direct measurements carried out in different seasons by means of a flux chamber to the case study of Palermo (IT) landfill (Bellolampo). The results showed that the flux chamber method is reliable and easy to perform, and the contoured flux maps, obtained by processing the measured data were found to be a suitable tool for identifying areas with abnormal (high) emissions. Further, it was found that a relationship between methane emission rates and landfill side slope can be established. Concerning the influence of the temporary HDPE cover system on CH4 recovery efficiency, it contributed to a significant decrease of the free surface area available for uncontrolled emissions; this aspect, coupled to the increase of the CH4 volumes collected by the LFG recovery system, led to a significant increase of the recovery efficiency., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

Membrane bioreactors (MBR) are being increasingly used for wastewater treatment. Mathematical modeling of MBR systems plays a key role in order to better explain their characteristics. Several MBR models have been presented in the literature focusing on different aspects: biological models, models which include soluble microbial products (SMP), physical models able to describe the membrane fouling and integrated models which couple the SMP models with the physical models. However, only a few integrated models have been developed which take into account the relationships between membrane fouling and biological processes. With respect to biological phosphorus removal in MBR systems, due to the complexity of the process, practical use of the models is still limited. There is a vast knowledge (and consequently vast amount of data) on nutrient removal for conventional-activated sludge systems but only limited information on phosphorus removal for MBRs. Calibration of these complex integrated models still remains the main bottleneck to their employment. The paper presents an integrated mathematical model able to simultaneously describe biological phosphorus removal, SMP formation/degradation and physical processes which also include the removal of organic matter. The model has been calibrated with data collected in a UCT-MBR pilot plant, located at the Palermo wastewater treatment plant, applying a modified version of a recently developed calibration protocol. The calibrated model provides acceptable correspondence with experimental data and can be considered a useful tool for MBR design and operation.

The paper presents a 1D mathematical model for the simulation of the percolation fluxes throughout a landfill for municipal solid waste (MSW). Specifically, the model was based on mass balance equations, that enable simulation of the formation of perched leachate zones in a landfill for MSW. The model considers the landfill divided in several layers evaluating the inflow to and outflow from each layer as well as the continuous moisture distribution. The infiltration flow was evaluated by means of the Darcy's law for an unsaturated porous medium, while the moisture distribution evaluation has been carried out on the basis of the theory of the vertically distributed unsaturated flow. The solution of the model has been obtained by means of the finite difference method. The model has been applied to a semi-idealized landfill located in Palermo landfill (Bellolampo). Specifically, field measurements were conducted to determine the relationship between waste density and applied vertical strain. This relationship was then used to relate vertical strain to waste porosity. The inflow rate to the system was simulated via a synthetic hyetograph whose characteristics have been identified in a previous hydrologic study. Three simulations, each with a different initial moisture content, were conducted. The model results showed a different response of the landfill in terms both of flow rates throughout the landfill and moisture profile. Indeed, the initial moisture content drastically influenced not only the formation of perched leachate zones but also their extension. The model can be a useful tool in predicting potential for the formation of perched leachate zones., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Methane (CH(4)) diffuse emissions from Municipal Solid Waste (MSW) landfills represent one of the most important anthropogenic sources of greenhouse gas. CH(4) is produced by anaerobic biodegradation of organic matter in landfilled MSW and constitutes a major component of landfill gas (LFG). Gas recovery is a suitable method to effectively control CH(4) emissions from landfill sites and the quantification of CH(4) emissions represents a good tool to evaluate the effectiveness of a gas recovery system in reducing LFG emissions. In particular, LFG emissions can indirectly be evaluated from mass balance equations between LFG production, recovery and oxidation in the landfill, as well as by a direct approach based on LFG emission measurements from the landfill surface. However, up to now few direct measurements of landfill CH(4) diffuse emissions have been reported in the technical literature. In the present study, both modeling and direct emission measuring methodologies have been applied to the case study of Bellolampo landfill located in Palermo, Italy. The main aim of the present study was to evaluate CH(4) diffuse emissions, based on direct measurements carried out with the flux accumulation chamber (static, non-stationary) method, as well as to obtain the CH(4) contoured flux map of the landfill. Such emissions were compared with the estimate achieved by means of CH(4) mass balance equations. The results showed that the emissions obtained by applying the flux chamber method are in good agreement with the ones derived by the application of the mass balance equation, and that the evaluated contoured flux maps represent a reliable tool to locate areas with abnormal emissions in order to optimize the gas recovery system efficiency., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Urban sources of water pollution have often been cited as the primary cause of poor water quality in receiving water bodies (RWB), and recently many studies have been conducted to investigate both continuous sources, such as wastewater-treatment plant (WWTP) effluents, and intermittent sources, such as combined sewer overflows (CSOs). An urban drainage system must be considered jointly, i.e., by means of an integrated approach. However, although the benefits of an integrated approach have been widely demonstrated, several aspects have prevented its wide application, such as the scarcity of field data for not only the input and output variables but also parameters that govern intermediate stages of the system, which are useful for robust calibration. These factors, along with the high complexity level of the currently adopted approaches, introduce uncertainties in the modelling process that are not always identifiable. In this study, the identifiability analysis was applied to a complex integrated catchment: the Nocella basin (Italy). This system is characterised by two main urban areas served by two WWTPs and has a small river as the RWB. The system was simulated by employing an integrated model developed in previous studies. The main goal of the study was to assess the right number of parameters that can be estimated on the basis of data-source availability. A preliminary sensitivity analysis was undertaken to reduce the model parameters to the most sensitive ones. Subsequently, the identifiability analysis was carried out by progressively considering new data sources and assessing the added value provided by each of them. In the process, several identifiability methods were compared and some new techniques were proposed for reducing subjectivity of the analysis. The study showed the potential of the identifiability analysis for selecting the most relevant parameters in the model, thus allowing for model simplification, and in assessing the impact of data sources for model reliability, thus guiding the analyst in the design of future monitoring campaigns. Further, the analysis showed some critical points in integrated urban drainage modelling, such as the interaction between water quality processes on the catchment and in the sewer, that can prevent the identifiability of some of the related parameters., (Copyright © 2010 Elsevier Ltd. All rights reserved.)

Foaming in Membrane BioReactor (MBR) is a frequently discussed topic. Some authors reported that the phenomenon is due to filamentous organisms, like at Conventional Activated Sludge (CAS) plants. However, in recent years, other authors reported that the Extra-cellular Polymer Substances (EPSs) concentration is an important factor for controlling foam as well. Nevertheless, even if a number of MBR plants are affected by foaming, presently there are no suitable methods to evaluate the phenomenon. To facilitate the study of this controversial phenomenon in an MBR system, certain foam tests proposed in the past for CASPs were investigated. The results of the tests were able to adequately measure quantity, stability and quality of the foam. In particular, the Scum Index increased proportionally with the EPS concentration and mixed liquor viscosity; Foam Power was mainly correlated with the protein concentration of in the EPS; Foam Rating was also correlated with the EPS concentration., (Copyright © 2010 Elsevier Ltd. All rights reserved.)

In the past, emission standard indicators have been adopted by environmental regulation authorities in order to preserve the quality of a receiving water body. Such indicators are based on the frequency or magnitude of a polluted discharge that may be continuous or intermittent. In order to properly maintain the quality of receiving waters, the Water Framework Directive, following the basic ideas of British Urban Pollution Manual, has been established. The Directive has overtaken the emission-standard concept, substituting it with the stream-standard concept that fixes discharge limits for each polluting substance depending on the self-depurative characteristics of receiving waters. Stream-standard assessment requires the deployment of measurement campaigns that can be very expensive; furthermore, the measurement campaigns are usually not able to provide a link between the receiving water quality and the polluting sources. Therefore, it would be very useful to find a correlation between the quality status of the natural waters and the emission-based indicators. Thus, this study is aimed to finding a possible connection between the receiving water quality indicators drawn by environmental regulation authorities and emission-based indicators while considering both continuous (i.e. from the wastewater treatment plants) and intermittent pollution discharges (mainly from combined sewer overflows). Such research has been carried out by means of long-term analysis adopting a holistic modelling approach. The different parts of the integrated urban drainage system were modelled by a parsimonious integrated model. The analysis was applied to an ephemeral river bounding Bologna (Italy). The study concluded that the correlation between receiving water quality and polluting emissions cannot be generally stated. Nevertheless, specific analyses on polluting emissions were pointed out in the study highlighting cause-effect link between polluting sources and receiving water quality.

Over the last decade new technologies are emerging even more for wastewater treatment. Among the new technologies, a recent possible solution regards Moving Bed Biofilm Reactors (MBBRs) that represent an effective alternative to conventional processes. More specifically such systems consist in the introduction of plastic elements inside the aerobic reactor as carrier material for the growth of attached biomass. Recently, one of the mostly used alternatives is to couple the Moving Bed Biofilm Reactor (MBBR) process with the conventional activated sludge process, and the resulting process is usually called HMBBR (Hybrid MBBR). In the MBBR process the biofilm grows attached on small plastic elements that are kept in constant motion throughout the entire volume of the reactor. Indeed, in such a system, a competition between the two biomasses, suspended and attached, can arise for the availability of the substrates, leading, as a consequence, to a modification in the biokinetic parameters of the two biomasses, compared to that of a pure suspended or attached biomass process. This paper presents the first results of a study aimed at estimating the kinetic heterotrophic constants in a HMBBR pilot plant using respirometric techniques. The pilot plant was built at the Acqua dei Corsari (Palermo) wastewater treatment plant and consisted of two parallel lines realized in a pre-anoxic scheme, in one of which the carrier material was added to the aerobic reactor with a filling ratio of 30%.

Water quality modelling is of crucial importance for the assessment of physical, chemical, and biological changes in water bodies. Mathematical approaches to water modelling have become more prevalent over recent years. Different model types ranging from detailed physical models to simplified conceptual models are available. Actually, a possible middle ground between detailed and simplified models may be parsimonious models that represent the simplest approach that fits the application. The appropriate modelling approach depends on the research goal as well as on data available for correct model application. When there is inadequate data, it is mandatory to focus on a simple river water quality model rather than detailed ones. The study presents a parsimonious river water quality model to evaluate the propagation of pollutants in natural rivers. The model is made up of two sub-models: a quantity one and a quality one. The model employs a river schematisation that considers different stretches according to the geometric characteristics and to the gradient of the river bed. Each stretch is represented with a conceptual model of a series of linear channels and reservoirs. The channels determine the delay in the pollution wave and the reservoirs cause its dispersion. To assess the river water quality, the model employs four state variables: DO, BOD, NH(4), and NO. The model was applied to the Savena River (Italy), which is the focus of a European-financed project in which quantity and quality data were gathered. A sensitivity analysis of the model output to the model input or parameters was done based on the Generalised Likelihood Uncertainty Estimation methodology. The results demonstrate the suitability of such a model as a tool for river water quality management.

The paper presents the comparison between the traditional activated sludge system (AS) and a hybrid moving bed biofilm reactor (HMBBR). In particular, an experimental campaign has been carried out at the WWTP in Palermo (Italy), on a pilot plant consisting of two pre-anoxic schemes. The aerated tank of the HMBBR line was filled with suspended carriers (AnoxKaldnes K1), with a 30% filling ratio. The hydraulic load of the HMBBR line was increased up to two times the AS one. Further, in order to distinguish the additional contribution of the attached biomass for the HMBBR system, in the two lines the Mixed Liquor Suspended Solids (MLSS) was maintained as equal as possible. The monitoring period lasted three months during which several parameters were monitored. The obtained results showed a good treatment ability of the HMBBR system, referring to the organic matter removal as well as to the ammonium removal. In particular, in spite of the increase of the hydraulic load for the HMBBR line, the two systems showed a similar performance in terms of both organic and nitrogen removal. The results demonstrate the higher treatment capacity of the HMBBR addressing such system as an effective technology for the upgrading of overloaded wastewater treatment plants.

Urban water quality management often requires use of numerical models allowing the evaluation of the cause-effect relationship between the input(s) (i.e. rainfall, pollutant concentrations on catchment surface and in sewer system) and the resulting water quality response. The conventional approach to the system (i.e. sewer system, wastewater treatment plant and receiving water body), considering each component separately, does not enable optimisation of the whole system. However, recent gains in understanding and modelling make it possible to represent the system as a whole and optimise its overall performance. Indeed, integrated urban drainage modelling is of growing interest for tools to cope with Water Framework Directive requirements. Two different approaches can be employed for modelling the whole urban drainage system: detailed and simplified. Each has its advantages and disadvantages. Specifically, detailed approaches can offer a higher level of reliability in the model results, but can be very time consuming from the computational point of view. Simplified approaches are faster but may lead to greater model uncertainty due to an over-simplification. To gain insight into the above problem, two different modelling approaches have been compared with respect to their uncertainty. The first urban drainage integrated model approach uses the Saint-Venant equations and the 1D advection-dispersion equations, for the quantity and for the quality aspects, respectively. The second model approach consists of the simplified reservoir model. The analysis used a parsimonious bespoke model developed in previous studies. For the uncertainty analysis, the Generalised Likelihood Uncertainty Estimation (GLUE) procedure was used. Model reliability was evaluated on the basis of capacity of globally limiting the uncertainty. Both models have a good capability to fit the experimental data, suggesting that all adopted approaches are equivalent both for quantity and quality. The detailed model approach is more robust and presents less uncertainty in terms of uncertainty bands. On the other hand, the simplified river water quality model approach shows higher uncertainty and may be unsuitable for receiving water body quality assessment.

Numerical modelling can be a useful tool to assess a receiving water body's quality state. Indeed, the use of mathematical models in river water quality management has become a common practice to show the cause-effect relationship between emissions and water body quality and to design as well as assess the effectiveness of mitigation measures. In the present study, a hydrodynamic river water quality model is presented. The model consists of a quantity and a quality sub-model. The quantity sub-model is based on the Saint Venant equations. The solution of the Saint Venant equations is obtained by means of an explicit scheme based on space-time conservation. The method considers the unification of space and time and the enforcement of flux conservation in both space and time. On the other hand, the quality sub-model is based on the advection dispersion equation. Particularly, the principle of upstream weighting applied to finite difference methods is employed. This method enable us to reduce the numerical dispersion avoiding oscillation phenomena. The optimal weighting coefficient was calculated on the basis of the mesh Peclet number. Regarding the quality processes, the model takes into account the main physical/chemical processes; these are degradation of dissolved carbonaceous substances, ammonium oxidation, algal uptake and denitrification, dissolved oxygen balance, including depletion by degradation processes and supply by physical reaeration and photosynthetic production. To properly simulate the river water quality, four state variables were considered: DO, BOD, NH4, and NO. The model was applied to the Savena River (Italy), which is the focus of a European-financed project for which quantity and quality data were gathered. A sensitivity analysis of the model output compared to the model input or parameters was carried out.

In the past three decades, scientific research has focused on the preservation of water resources, and in particular, on the polluting impact of urban areas on natural water bodies. One approach to this research has involved the development of tools to describe the phenomena that take place on the urban catchment during both wet and dry periods. Research has demonstrated the importance of the integrated analysis of all the transformation phases that characterise the delivery and treatment of urban water pollutants from source to outfall. With this aim, numerous integrated urban drainage models have been developed to analyse the fate of pollution from urban catchments to the final receiving waters, simulating several physical and chemical processes. Such modelling approaches require calibration, and for this reason, researchers have tried to address two opposing needs: the need for reliable representation of complex systems, and the need to employ parsimonious approaches to cope with the usually insufficient, especially for urban sources, water quality data. The present paper discusses the application of a be-spoke model to a complex integrated catchment: the Nocella basin (Italy). This system is characterised by two main urban areas served by two wastewater treatment plants, and has a small river as the receiving water body. The paper describes the monitoring approach that was used for model calibration, presents some interesting considerations about the monitoring needs for integrated modelling applications, and provides initial results useful for identifying the most relevant polluting sources.

The objective of this paper is the definition of a methodology to evaluate the impact of the temporal resolution of rainfall measurements in urban drainage modelling applications. More specifically the effect of the temporal resolution on urban water quality modelling is detected analysing the uncertainty of the response of rainfall-runoff modelling. Analyses have been carried out using historical rainfall-discharge data collected for the Fossolo catchment (Bologna, Italy). According to the methodology, the historical rainfall data are taken as a reference, and resampled data have been obtained through a rescaling procedure with variable temporal windows. The shape comparison between 'true' and rescaled rainfall data has been carried out using a non-dimensional accuracy index. Monte Carlo simulations have been carried out applying a parsimonious urban water quality model, using the recorded data and the resampled events. The results of the simulations were used to derive the cumulative probabilities of quantity and quality model outputs (peak discharges, flow volume, peak concentrations and pollutant mass) conditioned on the observation according to the GLUE (Generalized Likelihood Uncertainty Estimation) methodology. The results showed that when coarser rainfall information is available, the model calibration process is still efficient even if modelling uncertainty progressively increases especially with regards to water quality aspects.

In this study, diffuse and point source pollutant loads were evaluated using an Italian case study: the Nocella catchment, which has been subject to extensive monitoring. The Nocella catchment is located in Sicily (Italy) and has an area of about 60 km(2). The river receives wastewater and stormwater from two urban areas drained by combined sewers. The two sewer systems, two wastewater treatment plants and a river reach were monitored during both dry and wet weather periods. Thereafter, an integrated catchment-scale model was applied to simulate point pollutant sources, i.e., pollution coming from the urban drainage system, and nonpoint pollutant sources, i.e., pollution coming from agricultural and wildlife activities. Different models were combined and long-term simulations were carried out in order to reconstruct the total pollutant loads discharged into the receiving water body and identify the roles of the different pollutant sources. This study demonstrates the complexity of water quality assessment in partially urbanised natural basins where neither point nor nonpoint sources can be neglected. Point sources are mainly responsible for acute oxygen demanding polluting impact during wet weather periods, and both point and nonpoint sources are responsible for the impact of nutrients on the receiving water body.

In recent years, limitations linked to traditional urban drainage schemes have been pointed out and new approaches are developing introducing more natural methods for retaining and/or disposing of stormwater. These mitigation measures are generally called Best Management Practices or Sustainable Urban Drainage System and they include practices such as infiltration and storage tanks in order to reduce the peak flow and retain part of the polluting components. The introduction of such practices in urban drainage systems entails an upgrade of existing modelling frameworks in order to evaluate their efficiency in mitigating the impact of urban drainage systems on receiving water bodies. While storage tank modelling approaches are quite well documented in literature, some gaps are still present about infiltration facilities mainly dependent on the complexity of the involved physical processes. In this study, a simplified conceptual modelling approach for the simulation of the infiltration trenches is presented. The model enables to assess the performance of infiltration trenches. The main goal is to develop a model that can be employed for the assessment of the mitigation efficiency of infiltration trenches in an integrated urban drainage context. Particular care was given to the simulation of infiltration structures considering the performance reduction due to clogging phenomena. The proposed model has been compared with other simplified modelling approaches and with a physically based model adopted as benchmark. The model performed better compared to other approaches considering both unclogged facilities and the effect of clogging. On the basis of a long-term simulation of six years of rain data, the performance and the effectiveness of an infiltration trench measure are assessed. The study confirmed the important role played by the clogging phenomenon on such infiltration structures.

Over the last few years there has been a growing attention regarding the receiving water body quality state. As a matter of a fact, the Directive 91/271 of the European Union (EU) replaced the "emission standard" concept that fixes discharge limits depending on polluting emission characteristics, with the "stream standard" concept that fixes discharge limits for each polluting substance depending on self-depurative characteristics of the RWB. In this context, several WWTPs need to be upgraded in order to meet stricter effluent limits. The need of WWTP upgrading was also emphasized by the growing urbanization that have led, in most cases, to get overloaded WWTP due to an overcoming of the maximum WWTP capacity. In order to upgrade existing WWTP basically two main possibilities can be chosen: building new tanks or modify the WWTP by introducing new technologies such as the HMBBR systems. In this paper, such latter possibility was explored and as a case study an existing Italian WWTP (Acqua dei Corsari) located in Palermo (IT) was analysed. The main goal was to test the effectiveness of HMBBR systems with respect to the WWTP upgrading. The survey was carried out by means of model simulation and an HMBBR pilot plant. This latter was employed for the evaluation of the model parameters as well as kinetic coefficients for the HMBBR. The model results are encouraging towards the WWTP upgrading by means of HMBBR. As a matter of fact, the model simulation results showed that the WWTP maximum capacity can be upgraded from 480,000 up to one million PE.

Sewer systems convey mostly dry weather flow, coming from domestic and industrial sanitary sewage as well as infiltration flow, and stormwater due to meteoric precipitations. Traditionally, in urban drainage two types of sewer systems are adopted: separate and combined sewers. The former convey dry and wet weather flow separately into two different networks, while the latter convey dry and wet weather flow together. Which is the best solution in terms of cost-benefit analysis still remains a controversial subject. The present study was aimed at comparing the pollution loads discharged to receiving bodies by Wastewater Treatment Plant (WWTP) and Combined Sewer Overflow (CSO) for different kinds of sewer systems (combined and separate). To accomplish this objective, a comparison between the two systems was carried out using results from simulations of catchments characterised by different dimensions, population densities and water supply rate. The analysis was based on a parsimonious mathematical model able to simulate the sewer system as well as the WWTP during both dry and wet weather. The rain series employed for the simulations was six years long. Several pollutants, both dissolved and particulate, were modelled. The results confirmed the uncertainties in the choice of one system versus the other, emphasising the concept that case-by-case solutions have to be undertaken. Further, the compared systems showed different responses in terms of effectiveness in reducing the discharged mass to the RWB in relation to the particular pollutant taken into account.

Uncertainty analysis in integrated urban drainage modelling is of growing importance in the field of water quality. However, only few studies deal with uncertainty quantification in urban drainage modelling; furthermore, the few existing studies mainly focus on quantitative sewer flow modelling rather than uncertainty in water quality aspects. In this context, the generalised likelihood uncertainty estimation (GLUE) methodology was applied for the evaluation of the uncertainty of an integrated urban drainage model and some of its subjective hypotheses have been explored. More specifically, the influence of the subjective choice of the acceptability threshold has been detected in order to gain insights regarding its effect on the model results. The model has been applied to the Savena case study (Bologna, Italy) where water quality and quantity data were available. The model results show a strong influence of the acceptability threshold selection and confirm the importance of modeller's experience in the application of GLUE uncertainty analysis.

The performance of detention tanks with different characteristics (volume, on-line and off-line arrangement) has been evaluated according to the results of a continuous simulation. The conceptual simplified model for sewer system simulation (COSMOSS) model has been used to simulate the operation of the tanks. The differences between the performance of on-line and off-line tanks and the influence of the characteristics of different catchments have been examined. According to the results of the simulations detention tanks demonstrated good performances in total suspended solids retention and this evenience can certainly help to prevent water pollution of receiving water bodies in urban areas, even if the differences between the catchments, especially regard to the first flush effect, influence the performance of the tanks. Anyway considerable good efficiencies can be obtained with tank volumes of about 30-50 m(3)/ha(imp), in terms of number, maximum concentrations and duration of overflows, generally not guaranteed only with overflow devices.