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Objective: Whether peripheral blood 5-hydroxytrptamine (5-HT) levels serve as biomarker for depression diagnosis/response evaluation has not been well determined. This work was explored to address this inconclusive issue., Methods: Animals were randomized into normal control group (NC, n = 10) and chronic unpredictable mild stress model group (CUMS-model, n = 20), respectively. Animals in CUMS-model group were subjected to chronic stress, then they were randomly subdivided into CUMS subgroup and CUMS + fluoxetine subgroup (CUMS + FLX). After FLX treatment, blood and tissues were collected. 5-HT and relevant protein expression were measured., Results: In mice model, there was a significant increase in serum and a significant reduction in plasma 5-HT levels in CUMS-model group versus NC group, while platelet 5-HT levels change little. After FLX treatment, serum and platelet 5-HT levels were significantly decreased in CUMS + FLX subgroup, while plasma 5-HT levels had not much change versus CUMS subgroup. Chronic stress enhanced colon and platelet serotonin transporter (SERT) expression and FLX treatment mitigated SERT expression. In rats' model, there was a significant increase in serum 5-HT levels while plasma and platelet 5-HT levels showed little change in CUMS group versus NC group. After FLX treatment, serum, plasma and platelet 5-HT levels were significantly decreased in CUMS + FLX subgroup versus CUMS subgroup. The profile of relevant proteins expression changed by FLX were like those in mice., Conclusion: Serum 5-HT levels might serve as a potential biomarker for depression diagnosis, meanwhile serum and platelet 5-HT levels might respond to antidepressant treatment.

In order to maintain the ecological health of rivers and balance ecological and economic benefits, the study focuses on the Gorge Section of the Yongding River. The Lyon method was utilized to determine suitable ecological flow values. An optimized scheduling model for cascade hydropower stations was established to meet ecological flow requirements. Various scheduling scenarios were established to derive the optimized dispatching schemes for cascade hydropower stations. The results show that the appropriate natural ecological flow for the Gorge Section of the Yongding River, ranges from 4.05 to 9.86 m 3 /s. Under Scenario 1 (outbound flow from Guanting Reservoir: 40.5 m 3 /s, ecological flow: 4.50 m 3 /s), Scenario 2 (40.5 m 3 /s, 6.68 m 3 /s), Scenario 3 (80.0 m 3 /s, 4.50 m 3 /s), and Scenario 4 (80.0 m 3 /s, 6.68 m 3 /s), the optimized scheduling schemes resulted in comprehensive system electricity generation of 10.4132 million kW.h, 9.1727 million kW.h, 18.6214 million kW.h, and 18.4660 million kW.h, respectively., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Mixed tin-lead perovskite solar cells can reach band gaps as low as 1.2 eV, offering high theoretical efficiency and serving as base materials for all-perovskite tandem solar cells. However, instability and high defect densities at the interfaces, particularly the buried surface, have limited performance improvements. In this work, we present the modification of the bottom perovskite interface with multifunctional hydroxylamine salts. These salts can effectively coordinate the different perovskite components, having critical influences in regulating the crystallization process and passivating defects of varying nature. The surface modification reduced traps at the interface and prevented the formation of excessive lead iodide, enhancing the quality of the films. The modified devices presented fill factors reaching 81 % and efficiencies of up to 23.8 %. The unencapsulated modified devices maintained over 95 % of their initial efficiency after 2000 h of shelf storage., (© 2024 Wiley-VCH GmbH.)

Background: Food safety has become a serious global concern. Therefore, there is a need for effective detection technologies in this field. Currently, the development of effective on-site detection techniques is extremely important for food safety. However, the traditional on-site detection methods currently lack effective signal amplification. Herein, the aim of this study was to construct a nanopore-based label-free CRISPR/Cas12a system for the detection of Zearalenone (ZEN). The method is expected to be highly sensitive for portable detection of ZEN in food., Results: The proposed strategy was mainly involved three steps, including the displacement of the target DNA, the triggering of the cleavage of hairpin DNA probes (probes 1) by the trans-cleavage of CRISPR/Cas12a, and the generation of a measurable nanopore current signal. The probes 1 and DNA after the cleavage of probes 1 (probes 2) produce different characteristic nanopore signals as they pass through the nanopore. The established method achieved a low limit of detection (LOD) of 6.52 fM for ZEN and a wide liner range under optimized conditions. Furthermore, the practical applicability of this method was verified in real maize samples and showed good recoveries (90.68-101.98 %) and low relative standard deviations (RSD) (9.21-9.72 %). Therefore, this method is a promising option for rapid and ultrasensitive detection of ZEN., Significance and Novelty: The study presented a portable nanopore-based CRISPR/Cas12a signal amplification detection system for the detection of ZEN in food, which had a low LOD and the advantages of rapid, portability, and on-site detection potential. In conclusion, the method presented a promising prospect and universal platform for the detection of ZEN and other mycotoxins, offering a novel insight into on-site food safety detection., Competing Interests: Declaration of competing interest The authors declare that they do not have any competing financial or personal interests which can influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Label-free surface-enhanced Raman spectroscopy (SERS) is capable of capturing rich compositional information from complex biosamples by providing vibrational spectra that are crucial for biosample identification. However, increasing complexity and subtle variations in biological media can diminish the discrimination accuracy of traditional SERS excited by a single laser wavelength. Herein, we introduce a multiwavelength SERS approach combined with machine learning (ML)-based classification to improve the discrimination accuracy of human urine specimens for bladder cancer (BCa) diagnosis. This strategy leverages the excitation-wavelength-dependent SERS spectral profiles of complex matrices, which are mainly attributed to wavelength-related vibrational changes in individual analytes and differences in the variation ratios of SERS intensity across different wavelengths among various analytes. By capturing SERS fingerprints under multiple excitation wavelengths, we can acquire more comprehensive and unique chemical information on complex samples. Further experimental examinations with clinical urine specimens, supported by ML algorithms, demonstrate the effectiveness of this multiwavelength strategy and improve the diagnostic accuracy of BCa and staging of its invasion with SERS spectra from increasing numbers of wavelengths. The multiwavelength SERS holds promise as a convenient, cost-effective, and broadly applicable technique for the precise identification of complex matrices and diagnosis of diseases based on body fluids.

Worldwide, women face a disproportionately heavy health burden than their male counterparts. Yet there is a chronic lack of research attention and investment in women's health, which may explain the absence of women-inclusive, let alone women-centred, understanding of the causes, consequences, and confounding factors that shape individuals' health and quality of life. In this manuscript, by analysing the U.S. National Health Expenditure data, we show how ingrained the issue of poor investment in women's health even in the 0-18 years age group in terms of per capita health expenditure. We hope this manuscript sheds light on how early-life poor health investment in women's health might be a potential cause for women's poor health in adulthood, and in turn, underscores the importance of investing resources into better understanding and improving women's health., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Association of Physicians. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

Background: Remnant Cholesterol (RC) has emerged as a significant risk factor for cardiovascular disease. However, the factors influencing RC levels remain incompletely understood. This research investigates smoking-a major modifiable risk factor-to elucidate its impact on RC levels and examine the mediating role of inflammation in this relationship., Methods: Using NHANES data from 1999 to 2018, this study analyzed the association between serum cotinine levels (a biomarker of smoking intensity) and RC in 8,829 participants aged 20 years and older. Through complex sampling design and adjustment for multiple covariables, we examined both linear and nonlinear relationships using linear regression models, restricted cubic splines (RCS), and subgroup analyses. Additionally, mediation analyses evaluated the role of inflammatory markers-neutrophils (NEU), monocytes (MON), lymphocytes (LYM), and platelets (PLT)-in this association., Results: The high cotinine exposure group demonstrated significantly elevated RC levels (β = 2.256, 95% CI: 1.401-3.112, p < 0.001) compared to the no/minimal exposure group. This positive association was particularly pronounced in females (p for interaction < 0.05). Restricted cubic spline analysis demonstrated a nonlinear, N-shaped relationship (p for nonlinearity < 0.05), with RC levels reaching their peak at cotinine concentrations of approximately 172 ng/mL. In the mediation analysis, inflammatory markers showed significant mediating effects: NEU (28%), LYM (14.1%), PLT (9.5%), and MON (6.9%) of the total effect., Conclusion: A significant positive association exists between cotinine and RC levels, moderated by sex. Inflammatory markers, particularly NEU, partially mediate this association., Competing Interests: Declarations. Ethics approval and consent to participate: This research complied with the Helsinki Declaration and used NHANES data which has undergone ethical review by NCHS. As a secondary analysis of this approved dataset following STROBE guidelines, no additional ethical approval was required. Consent for publication: Written informed consent for publication was obtained from all participants. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Intramuscular fat content is one of the most important factors affecting beef quality. However, the role of alternate polyadenylation (APA) in intramuscular fat deposition remains unclear. We compared APA events in muscle samples from high and low intramuscular fat (IMF) cattle, based on RNA-seq data. A total of 363 significant APAs were identified. Notably, the number of shortened 3'UTR events exceeded the number of lengthened 3'UTR events, and genes associated with shortened 3'UTR events were enriched in fatty acid metabolism-related pathways. Most APA events had alternative 3'UTR (aUTR) lengths of 200-300 bp. As the 3'UTR lengthened, the aUTR also lengthened (R2 = 0.79). These findings indicate that genes with longer 3'UTRs are more likely to be regulated by APA in the muscle of cattle with high IMF. To determine whether the identified APA events drove alterations in the expression of fat deposition-related genes, we analyzed the relationship between APA events and differentially expressed genes and identified several genes critical for fat deposition (e.g., PFKL and SLC1A5). Since miRNAs usually bind to the 3'UTR region of protein-coding genes and affect gene expression, we constructed an miRNA-APA network to detect several key miRNAs that may regulate fat deposition. We identified 10 important miRNAs that affect changes in IMF content, which may be gained (gained miRNA-binding sites) or lost (lost miRNA-binding sites) owing to 187 differential APA events. Our study characterized the APA profiles of cattle with high and low intramuscular fat content and provided further insights into the relationship between APA, miRNA, and fat deposition., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

Genotoxic and immunosuppressive characteristics are central to the carcinogenic profile of hexavalent chromium [Cr(VI)], with dysregulation of circulating exosomal miRNA potentially acting as oncogenes or tumor suppressors or participating in the carcinogenic landscape of heavy metals through immunomodulation. In this two-stage epidemiological investigation, we unveiled for the first time the perturbations of exosomal miRNAs among individuals exposed to Cr(VI), alongside their significant correlations with biomarkers of genetic injury (γ-H2AX positivity in circulating lymphocytes and the urinary 8-OHdG levels) and immunological indicators (immunosuppressive PD-1 expression), which was supported by validation in an external cohort. Employing a support vector machine model, we discerned that exosomal miRNAs, particularly miR-4467, miR-345-5p, miR-144-3p, and miR-206, exhibited a remarkable capacity to delineate the genetic damage stratum within the population with high precision, and the target genes predicted of these miRNAs further elucidated their intricate regulatory interplay with the effector biomarkers. Additionally, employing a Bayesian mediation framework, we observed the intermediary function of miR-4467 in the nexus between chromium exposure and the escalation of urinary 8-OHdG levels (mediation effect: 0.47, P < 0.05). Although our findings suggested a link between extracellular miRNAs and immunosuppressive biomarkers, this association did not achieve validation in the external cohort, possibly due to population heterogeneity. Collectively, this study advanced our understanding of the epigenetic orchestration of health hazards of Cr(VI) by exosomal miRNAs, shedding light on their expression signatures and their intricate interplay with Cr(VI)-induced genetic and immunological perturbations, thus providing novel perspectives on the toxic pathways of heavy metals.

Persistent infection with hepatitis B virus (HBV) often leads to disruptions in lipid metabolism. Apolipoprotein AII (apoAII) plays a crucial role in lipid metabolism and is implicated in various metabolic disorders. However, whether HBV could regulate apoAII and contribute to HBV-related dyslipidemia and the underlying mechanism remain unclear. This study revealed significant reductions in apoAII expression in HBV-expressing cell lines, the serum, and liver tissues of HBV-transgenic mice. The impact of HBV on apoAII is related to small hepatitis B virus surface antigen (SHBs). Overexpression of SHBs decreased apoAII levels in SHBs-expressing hepatoma cells, transgenic mice, and the serum of HBV-infected patients, whereas suppression of SHBs increased apoAII expression. Mechanistic investigations demonstrated that SHBs repressed the apoAII promoter activity through a HNF4α- and C/EBPγ-dependent manner; SHBs simultaneously upregulated C/EBPγ and downregulated HNF4α by inhibiting the PI3K/AKT signaling pathway through activating endoplasmic reticulum (ER) stress. Serum lipid profile assessments revealed notable decreases in high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC), and triglycerides (TG) in SHBs-transgenic mice compared to control mice. However, concurrent overexpression of apoAII in these mice effectively counteracted these reductions in lipid levels. In HBV patients, SHBs levels were negatively correlated with serum levels of HDL-C, LDL-C, TC, and TG, whereas apoAII levels positively correlated with lipid content. This study underscores that SHBs contributes to dyslipidemia by suppressing the PI3K/AKT pathway via inducing ER stress, leading to altered expression of HNF4α and C/EBPγ, and subsequently reducing apoAII expression.IMPORTANCEThe significance of this study lies in its comprehensive examination of how the hepatitis B virus (HBV), specifically through its small hepatitis B virus surface antigen (SHBs), impacts lipid metabolism-a key aspect often disrupted by chronic HBV infection. By elucidating the role of SHBs in regulating apolipoprotein AII (apoAII), a critical player in lipid processes and associated metabolic disorders, this research provides insights into the molecular pathways contributing to HBV-related dyslipidemia. Discovering that SHBs downregulates apoAII through mechanisms involving the repression of the apoAII promoter via HNF4α and C/EBPγ, and the modulation of the PI3K/AKT signaling pathway via endoplasmic reticulum (ER) stress, adds critical knowledge to HBV pathogenesis. The research also shows an inverse correlation between SHBs expression and key lipid markers in HBV-infected individuals, suggesting that apoAII overexpression could counteract the lipid-altering effects of SHBs, offering new avenues for understanding and managing the metabolic implications of HBV infection., Competing Interests: The authors declare no conflict of interest.

Human breathing is crucial for studying indoor environments and human health. Computational Fluid Dynamics (CFD) is a key tool for simulating human respiration. To enhance the accuracy of CFD simulations and reduce computation time, a new simulation strategy for human respiration is proposed in this paper. The effects of steady versus unsteady boundary conditions on simulation results were examined. For the unsteady boundary, sinusoidal exhalation velocities and non-inhalation gas were assumed, while the steady boundary involved constant velocities during both exhalation and inhalation phases. The jet center trajectory under different boundary conditions was analyzed and compared with experimental data. Additionally, variations in pollutant dispersion near the mouth under the two boundary conditions were discussed. Furthermore, the paper compared the calculation accuracy, calculation time and memory occupied by a single turbulence model or switching flow character models in human respiration simulation. Differences in exhaled gas vorticity and jet penetration depth across different flow models were identified. Finally, combined with the non-iterative algorithm, the optimal strategy of human respiration simulation was proposed. Results show that under the comprehensive consideration of calculation accuracy, calculation time and memory occupancy, using sinusoidal expiratory boundary conditions combined with the PISO algorithm, with the RNG k-ε model during expiratory phase, and switching into the laminar flow during inspiratory phase, is the optimal strategy of simulating human breathing., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Osteogenic differentiation of mesenchymal stem cells (MSCs) plays a pivotal role in the pathogenesis and treatment of bone-related conditions such as osteoporosis and bone regeneration. While the WW domain-containing coiled-coil adaptor (WAC) protein is primarily associated with transcriptional regulation and autophagy, its involvement in MSC osteogenesis remains unclear. Here, the data reveal that the levels of WAC are diminished in both osteoporosis patients and osteoporosis mouse models. It plays a pivotal function in facilitating MSC osteogenesis and enhancing new bone formation both in vitro and in vivo. Mechanistically, WAC promotes MSC osteogenesis by protecting PINK1, a crucial initiator of mitophagy, from ubiquitination-dependent degradation thereby activating mitophagy. Interestingly, WAC interacts with the TM domains of PINK1 and prevents the K137 site from ubiquitination modification. The study elucidates the mechanism by which WAC modulates MSC osteogenesis, binds to PINK1 to protect it from ubiquitination, and identifies potential therapeutic targets for osteoporosis and bone defect repair., (© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)

A dodder (Cuscuta) often simultaneously parasitizes two or more adjacent hosts. Phosphate (Pi) deficiency is a common stress for plants, and plants often interact with soil microbes, including arbuscular mycorrhizal fungi (AMF), to cope with Pi stress. Little is known about whether dodder transmits Pi deficiency-induced systemic signals between different hosts. In this study, dodder-connected plant clusters, each composed of two tobacco (Nicotiana tabacum) plants connected by a dodder, were established, and in each cluster, one of the two tobacco plants was treated with Pi starvation. AMF colonization efficiency, rhizosphere bacterial community, and transcriptome were analyzed in the other dodder-connected Pi-replete tobacco plant to study the functions of interplant Pi signals. We found that dodder transfers Pi starvation-induced systemic signals between host plants, resulting in enhanced AMF colonization, changes of rhizosphere bacterial communities, and alteration of transcriptomes in the roots of Pi-replete plants. Importantly, genetic analyses indicated that microRNA399s (miR399s) and strigolactones suppress the systemic Pi signals and negatively affect AMF colonization in the Pi-replete plants. These findings provide new insight into the ecological role of dodder in mediating host-host and host-microbe interactions and highlight the importance of strigolactone and miR399 pathways in systemic Pi signaling., (© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.)

The acidic electrocatalytic conversion of CO 2 to multi-carbon (C 2+ ) oxygenates is of great importance in view of enhancing carbon utilization efficiency and generating products with high energy densities, but suffering from low selectivity and activity. Herein, we synthesized Ag-Cu alloy catalyst with highly rough surface, by which the selectivity to C 2+ oxygenates can be greatly improved. In a strongly acidic condition (pH=0.75), the maximum C 2+ products Faradaic efficiency (FE) and C 2+ oxygenates FE reach 80.4 % and 56.5 % at -1.9 V versus reversible hydrogen electrode, respectively, with a ratio of FE C2+ oxygenates to FE ethylene up to 2.36. At this condition, the C 2+ oxygenates partial current density is as high as 480 mA cm -2 . The in situ spectra, control experiments and theoretical calculations indicate that the high generation of C 2+ oxygenates over the catalyst originates from its large surface roughness and Ag alloying., (© 2024 Wiley-VCH GmbH.)

Purpose: Threonine (Thr) can be involved in the synthesis of immunoglobulins, which play the role of immune regulation, Thr also has to improve intestinal morphology, adjust the sticky protein synthesis, maintain the intestinal barrier function, etc. The experiment aimed to investigate the effects of diets supplemented with different levels of Thr on growth performance and intestinal health of rabbits under lipopolysaccharide (LPS) stress conditions., Methods: A total of 180 healthy 35-day-old weaned New Zealand White rabbits were randomly assigned in a 2 × 3 factorial design to receive an intraperitoneal injection of 100 µg/kg BW LPS or saline and three diets with different levels of digestible threonine (0.43%, 0.54%, and 0.64%)., Results: The LPS challenge resulted in a reduction in body weight in rabbits at day 22, as well as a decrease in the serum d-lactic acid (D-LA) content and the number of goblet cells (GCs) in the jejunum. Additionally, the duodenum JAM2 and JAM3 were down-regulated. The expression of OCLN, ZO-1, and IL-2 in the jejunum, and CLDN, nuclear factor-κB (NF-κB) and ZO-1 mRNA in the ileum were also down-regulated. Furthermore, the duodenum TLR4 and IL-1β mRNA expression, while the jejunum exhibited an elevation in CLDN, TNF-α, and ileum TNF-α mRNA expression (P < 0.05). In the context of LPS challenge condition, dietary Thr addition was found to down-regulate the duodenum ZO-1 and jejunum CLDN mRNA expression of rabbits (P < 0.05). This was accompanied by an increase in ileum sIgA content and GCs number (P < 0.05). Additionally, dietary Thr addition resulted in a downregulation of duodenum TLR4, MyD88, NF-κB, TNF-α and IL-1β, jejunum MyD88, and IL-1β mRNA expression, as well as an up-regulation of ileum IL-10 mRNA expression in rabbits (P < 0.05)., Conclusion: In conclusion, the LPS challenge can result in intestinal inflammation and damage the integrity of the intestinal barrier in rabbits. Nevertheless, dietary Thr supplementation can alleviate the intestinal inflammatory response in rabbits challenged with LPS., Competing Interests: Declarations Ethical approval All animal protocols were approved by the Animal Care and Use Committee of Sichuan Agricultural University (Approval number: SICAU20211121). Conflict of interest The authors declare no competing financial interest., (© 2024. Springer-Verlag GmbH Germany, part of Springer Nature.)

Ednrb is specifically required to develop neural crest (NC) stem cell-derived lineages. However, it is still unknown why Ednrb signaling is only needed for the early development of melanoblasts in the skin and eye. We show that Ednrb is required for the proliferation of melanoblasts during early mouse development. To understand the mechanism of melanoblast proliferation, we found that the gene absent in melanoma 2 (Aim2) is upregulated in Ednrb-deficient NC cells by RNA-sequencing analysis. Consequently, the knockdown or knockout of Aim2 partially rescued the proliferation of Ednrb-deficient melanoblasts. Conversely, the overexpression of Aim2 in melanoblasts suppressed their proliferation. We further show that Ednrb signaling could act through the microRNA miR-196b to block the suppression of melanoblast proliferation by Aim2 in primary NC cell cultures. These results reveal the Ednrb-Aim2-AKT axis in regulating melanocyte development and suggest that Ednrb signaling functions as a negative regulator of Aim2, which inhibits the proliferation of melanoblasts in early development. These findings uncover a previously unreported role for Aim2 outside the inflammasome, showing that it is a significant regulator controlling NC stem cell-derived lineage development., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2024. Published by The Company of Biologists Ltd.)

RNAs play critical roles in most biological processes. Although the three-dimensional (3D) structures of RNAs primarily determine their functions, it remains challenging to experimentally determine these 3D structures due to their conformational heterogeneity and intrinsic dynamics. Cryogenic electron microscopy (cryo-EM) has recently played an emerging role in resolving dynamic conformational changes and understanding structure-function relationships of RNAs including ribozymes, riboswitches and bacterial and viral noncoding RNAs. A variety of methods and pipelines have been developed to facilitate cryo-EM structure determination of challenging RNA targets with small molecular weights at subnanometer to near-atomic resolutions. While a wide range of conditions have been used to prepare RNAs for cryo-EM analysis, correlations between the variables in these conditions and cryo-EM visualizations and reconstructions remain underexplored, which continue to hinder optimizations of RNA samples for high-resolution cryo-EM structure determination. Here we present a protocol that describes rigorous screenings and iterative optimizations of RNA preparation conditions that facilitate cryo-EM structure determination, supplemented by cryo-EM data processing pipelines that resolve RNA dynamics and conformational changes and RNA modeling algorithms that generate atomic coordinates based on moderate- to high-resolution cryo-EM density maps. The current protocol is designed for users with basic skills and experience in RNA biochemistry, cryo-EM and RNA modeling. The expected time to carry out this protocol may range from 3 days to more than 3 weeks, depending on the many variables described in the protocol. For particularly challenging RNA targets, this protocol could also serve as a starting point for further optimizations., Competing Interests: Competing interests The authors declare no competing interests., (© 2024. Springer Nature Limited.)

C points, circular polarization in momentum space, play crucial roles in chiral wave manipulations. However, conventional approaches of achieving intrinsic C points using photonic crystals with broken symmetries suffer from a low Q factor and high sensitivity to structural geometry, rendering them fragile and susceptible to perturbations and disorders. We report magneto-optical (MO) bound states in the continuum (BICs) with a symmetry-preserved planar photonic crystal. We achieve intrinsic C points at Γ point that are robust against variation in both structural geometry and external magnetic field. MO coupling between two modes induces Zeeman splitting, leading to MO BICs and quasi-BICs with circular eigenstates for high- Q chiral responses. Furthermore, switchable C point handedness and circular dichroism are enabled by reversing the magnetic field. These findings unveil BICs and quasi-BICs with circular eigenstates and on-demand control of C points, paving the way for advanced chiral wave manipulation with enhanced light-matter interaction.

Carbon-silicon-switch strategy, replacing one specific carbon atom in organic molecules with a silicon, has garnered significant interest for developing new functional molecules. However, the influence of a reaction regarding its selectivity and reactivity by carbon-silicon-switch strategy has far less been investigated. Here we discover an unusual carbon-silicon-switch effect in the enantioselective construction of silicon-stereogenic center. It is found that there has been a significant change in the desymmetrization reaction of silacyclohexadienones using asymmetric conjugate addition or oxidative Heck reaction with aryl/alkyl nucleophiles when compared with their carbon analogues cyclohexadienones. Specifically, the carbon-silicon-switch leads to a reversal in enantioselectivity with arylzinc as the nucleophile by the same chiral catalyst, and results in totally different reactivity with arylboronic acid as the nucleophile. Control experiments and density functional theory (DFT) calculations have shown that the unusual carbon-silicon-switch effect comes from the unique stereoelectronic feature of silicon., Competing Interests: Competing interests The authors declare no competing interests., (© 2024. The Author(s).)

To reduce the harm of water contamination incidents in drinking water source areas (DWSAs) and explore feasible approaches, the research developed a hydrodynamic water quality model for DWSAs based on two dimensional water quality and quantity equations, Geographic Information System (GIS), and Digital Elevation Model (DEM). The Heshangshan Drinking Water Source Area (HDWSA) in the Three Gorges Reservoir Area (TGRA) was selected as the research area, with total phosphorus (TP) as the representative pollutant in the water. The research investigated the changes in TP content during various hydrological phase under pollution incident, compared the duration and trends of TP concentration exceeding standard value before and after joint reservoir group dispatch. The results showed that the migration speed of TP pollutants varied from slowest to fastest in the following order: dry phase, recession phase, storage phase, and flood phase. Under pollution incident condition, the time demanded for TP content to meet standard value in each water phase was 36 min (dry phase), 33 min (recession phase), 30 min (storage phase), and 27 min (flood phase). The joint dispatch group 1-3 shortened the time required to meet standard value by 6-13 min (dry phase), 5-11 min (recession phase), 4-9 min (storage phase), and 3-7 min (flood phase). The trend of TP concentration before and after joint dispatch showed four stages: significant increase, sharp decrease, rapid decrease, and slow decrease. Joint dispatch of reservoir group can effectively reduced the TP concentration in DWSA under pollution incident., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Human space activities have been continuously increasing. Astronauts experiencing spaceflight are faced with health problems caused by special space environments such as microgravity, and the investigation of cell injury is fundamental. The development of a platform capable of cell culture and injury detection is the prerequisite for the investigation. Constructing a platform suitable for special conditions in space life science research is the key issue. The ground-based investigation is an indispensable part of the research. Accordingly, a simulated microgravity (SMG)-oriented integrated chip platform capable of 3D cell culture and in situ visual detection of superoxide anion radical (O 2 •- ) is developed. SMG can cause oxidative stress in human cells, and O 2 •- is one of the signaling molecules. Thus, a O 2 •- -responsive aggregation-induced emission (AIE) probe is designed, which shows high selectivity and sensitivity to O 2 •- . Moreover, the probe exhibits abilities of long-term and wash-free staining to cells due to the AIE behavior, which is precious for space cell imaging. Meanwhile, a chip with a high-aspect-ratio chamber for adequate medium storage for the lack of the perfusion system during the SMG experiment and a cell culture chamber which can integrate the extracellular matrix (ECM) hydrogel for the bioinspired 3D cell culture is fabricated. In addition, a porous membrane is introduced between the chambers to prevent the hydrogel from separating during the SMG experiment. The afforded AIE probe-ECM hydrogel-integrated chip can achieve 3D culturing of U87-MG cells and in situ fluorescent detection of endogenous O 2 •- in the cells after long-term staining under SMG. The chip provides a powerful and potential platform for ground-based investigation in space life science and biomedical research., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Lithium-sulfur batteries (LSBs) hold promise as the next-generation lithium-ion batteries (LIBs) due to their ultra-high theoretical capacity and remarkable cost-efficiency. However, these batteries suffer from the serious shuttle effect, challenging their practical application. To address this challenge, we have developed a unique interlayer (HCON@CNWF) composed of hollow cerium oxide nanorods (CeO 2 ) anchored to carbonized non-woven viscose fabric (CNWF), utilizing a straightforward template method. The prepared interlayer features a three-dimensional (3D) conductive network that serves as a protective barrier and enhances electron/ion transport. Additionally, the CeO 2 component effectively chemisorbs and catalytically transforms lithium polysulfides (LiPSs), offering robust chemisorption and activation sites. Moreover, the unique porous structure of the HCON@CNWF not only physically adsorbs LiPSs but also provides ample space for sulfur's volume expansion, thus mitigating the shuttle effect and safeguarding the electrode against damage. These advantages collectively contribute to the battery's outstanding electrochemical performance, notably in retaining a reversible capacity of 80.82 % (792 ± 5.60 mAh g -1 ) of the initial value after 200 charge/discharge cycles at 0.5C. In addition, the battery with HCON@CNWF interlayer has excellent electrochemical performance at high sulfur loading (4 mg cm -2 ) and low liquid/sulfur ratio (7.5 µL mg -1 ). This study, thus, offers a novel approach to designing advanced interlayers that can enhance the performance of LSBs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Background: Recent studies have shown that KIR + CD8 + T cells play a role in suppressing autoimmunity by eliminating pathogenic CD4 + T cells. However, their specific role in type 1 diabetes (T1D) remains unclear., Methods: In this study, we enrolled 108 patients diagnosed with T1D and 86 healthy individuals. We conducted flow cytometric analysis to examine the various subtypes of KIR + CD8 + T cells derived from peripheral blood mononuclear cells. Additionally, CD8 + T cells were isolated from the peripheral blood of T1D patients to assess the functions of different KIR + CD8 + T cell subtypes. To investigate the influence of lipids on the characteristics and activities of these T cell subtypes, the isolated CD8 + T cells were cultured with varying concentrations of palmitic acid (PA). Furthermore, we utilized an NSG (NOD scid gamma) mouse adoptive transfer model to assess the impact of dietary lipid intake on the functionality of KIR2DL5 + CD8 + T cells in vivo., Results: We observed variations in circulating KIR + CD8 + T cell subtypes between patients with T1D and healthy controls. Notably, we observed a significant negative correlation between the frequencies of circulating KIR + CD8 + T cells and the titers of ZnT8 autoantibodies in individuals with T1D. Among these subtypes, KIR2DL5 + CD8 + T cells demonstrated a positive association with dietary fat intake, characterized by increased perforin expression and reduced PD-1 expression. Importantly, KIR2DL5 + CD8 + T cells exhibited enhanced proliferative capacity compared to other KIR + CD8 + T cell subsets. Palmitic acid (PA) was found to enhance the activation of KIR2DL5 + CD8 + T cells and strengthened their ability to suppress CD4 + T cell proliferation in T1D patients. Moreover, dietary lipid intake significantly enhanced the functionality of KIR2DL5 + CD8 + T cells in an NSG mouse adoptive transfer model., Conclusion: Our findings suggest that lipid intake enhances the functionality of human KIR2DL5 + CD8 + T cells and may offer implications for immunotherapy in T1D., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The use of synthetic biology to construct engineered strains has provided new perspectives for addressing Pb contamination; however, the large-scale treatment of contaminants is still limited by high operating costs and technological constraints. This study introduces a novel technique for applying engineered yeast in the removal of heavy metals, offering a solution to the cost and process scale challenges associated with utilizing engineered yeast. Hydrogen sulfide-producing engineered yeast strains were constructed based on existing strategies by knocking out the gene encoding the O-acetyl-L-homoserine mercapturic enzyme, which plays a role in sulfate assimilation. To facilitate the transition of engineered yeast from laboratory settings to industrial applications while reducing operating costs and addressing process scale-up issues, we proposes a new operational technology for engineered yeast based on their mechanistic understanding and a response surface optimization approach. The development and application of low-cost engineered media provide important guidance for utilizing engineered yeast to tackle Pb-contaminated wastewater and for the production of PbS crystalline nanomaterials. The industrial culture system was designed using economical materials and, through the response surface methodology, achieved removal rates of 99.02 ± 0.06 % and 80.95 ± 9.68 % of Pb²⁺ from Pb acid electrolyte and industrial Pb wastewater, respectively. This study presents a new technological solution for cost control and process scale-up based on the bioregulatory mechanisms of engineered yeast, laying the groundwork for their industrial application. Furthermore, it offers essential parameters and theoretical support for the industrial applications of engineered yeast in Pb wastewater treatment., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Introduction: Targeted screening of inhibitors of key enzymes in the progression of diabetes from natural products is one of the effective methods for the treatment of diabetes. Polygala has been proved to reduce glucose levels; however, the bioactive compounds in Polygalae Radix (PR) that have anti-diabetic properties are unknown., Objective: The purpose of this study was to explore the material basis of the anti-diabetic effect of PR by inhibiting α-amylase through an online detection system and molecular docking., Methods: An online analysis platform was established and optimized for the screening of potent enzyme inhibitors from complex mixtures based on ultra-performance liquid chromatography-photodiode array-quadrupole-time-of-flight-mass spectrometry-α-amylase-fluorescence detector (UHPLC-PDA-Q-TOF-MS n -α-amylase-FLD) detection system and molecular docking, which could efficiently separate extracts, quickly detect α-amylase inhibitors, and determine their structures. Molecular docking confirms the inhibition of these compounds. The molecular interaction between α-amylase and the active compound was evaluated., Results: Among the 101 compounds identified, 28 compounds had a strong inhibitory effect on α-amylase. Molecular docking screening confirmed the inhibition of these compounds and evaluated the molecular interactions between α-amylase and 30 active compounds, which strongly supported the experimental results. Among the evaluated compounds, onjisaponin R (83) and polygalaxanthone III (11) have the strongest inhibitory activity to α-amylase (the binding energies were -9.639 and -8.972 kcal/mol, respectively) and are potential lead compounds against diabetes., Conclusion: This study proved the feasibility of using the existing platform to screen the active ingredients in PR extract, and provided a practical method for the rapid screening of potential anti-diabetic active ingredients in traditional Chinese medicine., (© 2024 John Wiley & Sons Ltd.)

HBO1, also known as KAT7 or MYST2, is a crucial histone acetyltransferase with diverse cellular functions. It typically forms complexes with protein subunits or cofactors such as MEAF6, ING4, or ING5, and JADE1/2/3 or BRPF1/2/3, where the BRPF or JADE proteins serve as the scaffold targeting histone H3 or H4, respectively. The histone acetylation mediated by HBO1 plays significant roles in DNA replication and gene expression regulation. Additionally, HBO1 catalyzes the modification of proteins through acylation with propionyl, butyryl, crotonyl, benzoyl, and acetoacetyl groups. HBO1 undergoes ubiquitination and degradation by two types of ubiquitin complexes and can also act as an E3 ubiquitin ligase for the estrogen receptor α (ERα). Moreover, HBO1 participates in the expansion of medullary thymic epithelial cells (mTECs) and regulates the expression of peripheral tissue genes (PTGs) mediated by autoimmune regulator (AIRE), thus inducing immune tolerance. Furthermore, HBO1 influences the renewal of hematopoietic stem cells and the development of neural stem cells significantly. Importantly, the overexpression of HBO1 in various cancers suggests its carcinogenic role and potential as a therapeutic target. This review summarizes recent advancements in understanding HBO1's involvement in acylation modification, DNA replication, ubiquitination, immunity, and stem cell renewal., Competing Interests: Declarations Ethics approval and consent to participate Not applicable. Consent for publication All authors approved the final manuscript and the submission to this journal. Competing interests The authors declare no competing interests., (© 2024. The Author(s).)

DNBSEQ employs a patterned array to facilitate massively parallel sequencing of DNA nanoballs (DNBs), leading to a considerable boost in throughput. By employing the ultra-high-density (UHD) array with an increased density of DNB binding sites, the throughput of DNBSEQ can be further expanded. However, the typical imaging system of the DNBSEQ sequencer is unable to resolve adjacent DNBs spaced smaller than the resolution limit, resulting in poor base-calling performance of the UHD array and hindering its practical application. In this study, we propose a deep-learning-based DNB image super-resolution network named DNBSRN to address this problem. DNBSRN has a specifically designed structure for DNB images and employs a histogram-matching-based preprocessing approach. For the eight DNB image datasets generated from the DNBSEQ sequencer using UHD arrays with 360 nm pitch, the base-calling performances are significantly improved after super-resolution reconstruction by DNBSRN and reached a comparable level to those of the regular density array. In terms of reconstruction speed, DNBSRN takes only 7.61 ms for an input image with 500 × 500 pixels, which minimizes its influence on throughput. Furthermore, compared with state-of-the-art super-resolution networks, DNBSRN demonstrates superior performance in terms of both the quality and speed of DNB image reconstruction. DNBSRN successfully addresses the DNB image super-resolution task. Integrating DNBSRN into the image analysis workflow of DNBSEQ will allow for the application of UHD array, hence enabling a considerable improvement in throughput as well as tremendous savings in unit reagent cost., Competing Interests: Competing interests DNBSRN is covered by a pending patent. Zhiwei Zhai, Hao Zhang, Zeyu Su, Yang Liu, Hongmin Chen, Yuxiang Li, and Mengzhe Shen are employees of BGI Research, a subsidiary of BGI Group. Junfeng Li declare no competing interests., (© 2024. The Author(s).)

Meat adulteration detection is crucial for ensuring food safety, protecting consumer rights, and maintaining market integrity. However, the current methods face challenges in achieving multiplexed detection through efficient signal conversion and output. This study introduces a nanopore-based approach for the simultaneous detection of multiple meat adulteration. Leveraging the interaction between D A D A dipeptide and vancomycin, we designed a series of biomimetic ssDNA reporters with D A D A-Van tags for multiplexed signal output. These ssDNA reporters can generate highly distinctive current blockage signals, which can be discriminated simultaneously by the current waveform. Combined with PCR-based strand displacement, ssDNA reporters can be released from magnetic beads in the presence of the target gene and are analyzed using α-hemolysin (α-HL) nanopores for multiplexed signal output. The signal frequency for each target gene has a linear relationship with the specific concentration range (for duck: 1 × 10 -3 ∼ 10 nmol/L, for pork: 1 × 10 -2 ∼ 10 nmol/L, and for chicken: 1 × 10 -3 ∼ 10 nmol/L). The limits of detection are as follows: 0.418 pmol/L for duck, 4.473 pmol/L for pork, and 0.531 pmol/L for chicken. This method effectively enables the simultaneous detection of adulterated chicken, pork, and duck meat in lamb samples and holds potential for broad applications in ensuring the authenticity and safety of meat products.

It is of particular importance to develop an effective method that possesses several merits simultaneously of rapid, ultrasensitive, portable, and on-site detection potential for food safety detection. Herein, we propose a clickase-mediated immunoassay based on nanopore and bionic signal labels for the detection of ricin. The introduction of Cu/Cys clickase and nanopore simultaneously effectively addressed the inherent limitations of natural enzymes and colorimetric signal output, respectively. Using this method, bionic signal labels can be easily formed through DNA and Gram-positive bacterial cell wall terminal peptide fragments (labeled by alkynyl and azide, respectively) and vancomycin. Translocation of the D-P@vancomycin through the nanopore generated highly specific oscillation current traces. This method showed a great on-site detection potential and superior analytical performance owing to the combination of the specificity of antibodies, high CuAAC click reaction catalytic efficiency of clickase, ultrasensitivity of the nanopore, and high signal resolution of D-P@vancomycin. Moreover, the practical applicability of the established method was also verified, achieving a limit of detection (LOD) down to 200.9 ag/mL with a wide linear relationship under the optimized conditions. In conclusion, this method is promising for rapid, portable, ultrasensitive, and on-site food safety detection.

The major challenge in preparing high-performance Cu 2 ZnSn(S,Se) 4 solar cells is the large open circuit voltage deficit (V OC -def). A new strategy utilizing the synergistic substitution of Ag and In dual cations has been proposed to simultaneously address the problems of undesirable interface band alignment and high-density detrimental bulk defects, obtaining decreased carrier recombination rate and increased minority carrier lifetime. The shorter In-S/Se bonds move the CBM higher by generating stronger repulsive force than the Sn-S/Se bonds, thus adjusting the interface band alignment. Ag substitution can effectively suppress Cu─Zn disorder, while Ag, In dual substitution can further passivate Sn-related defects and solve the issue of low carrier concentration in Ag single-substituted samples. Besides, the superior carrier property of In-Se materials significantly enhanced the device carrier lifetime and minority carrier diffusion length. The state-of-the-art air-solution-processed CZTSSe device without any addition treatment with 14.33% efficiency and 580 mV V OC is obtained, possessing the lowest V OC -def value currently available in the CZTSSe field (V OC /V OC SQ = 64.7%). This work emphasizes the synergistic modulation of band alignment, defect level, grain growth, and carrier transportation by dual cation substitution, which paves a convenient and effective way to realize high-performance solar cells and photovoltaic devices., (© 2024 Wiley‐VCH GmbH.)

Postovulatory aging of oocytes involves a series of deleterious molecular and cellular changes, which adversely affect oocyte maturation, fertilization, and early embryonic development. Petunidin-3-O-(6-O-pcoumaroyl)-rutinoside-5-O-glucoside (PrG), the main active ingredient of anthocyanin, exerts antioxidant effects. This study investigated whether PrG supplementation could delay postovulatory oocyte aging by alleviating oxidative stress. Our results showed that PrG supplementation decreased the number of abnormal morphology oocytes and improved the oxidative stress of aged oocytes by facilitating the reduction of the reactive oxygen species, the increase in glutathione content, and the recovery of expression of antioxidant-related gene expression. In addition, PrG treatment recovered mitochondrial dysfunction, including mitochondrial distribution, mitochondrial membrane potential and adenosine triphosphate in aged oocytes. PrG-treated oocytes returned to normal levels of cytoplasmic and mitochondrial calcium. Notably, PrG inhibited early apoptosis in aged oocytes. RNA-seq and qRT-PCR results revealed that PrG ameliorated oxidative stress injury in postovulatory aging oocytes of mice via the putrescine pathway. In conclusion, in vitro PrG supplementation is a potential therapy for delaying postovulatory oocyte aging., Competing Interests: Declaration of competing interest All the authors affirm that the research was carried out without any commercial or financial affiliations that could create a conflict of interest., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Background: While previous reports have shown that hepatitis B virus (HBV) infection affects lipid metabolism and vice versa, the impact of dyslipidemia on the functional cure of HBV infection following peginterferon alfa (PegIFNα) therapy remains unknown. Hence, this study aimed to investigate the effect of dyslipidemia on hepatitis B surface antigen (HBsAg) clearance and develop a nomogram model for predicting patients for whom PegIFNα therapy is indicated., Methods: A total of 160 nucleos(t)ide analogues (NAs)- experienced chronic hepatitis B (CHB) patients treated with PegIFNα (180 µg/week) were enrolled in this study. The relationship between serum lipid and HBsAg clearance was analysed. Univariate and multivariate COX analyses were used to construct and plot the nomogram model. The area under the receiver operating characteristic curve (AUC) and calibration curve were used to evaluate the discrimination and calibration of the model, respectively., Results: After 48 weeks of PegIFNα therapy, a total of 33 patients in the cohort achieved HBsAg clearance. Univariate and multivariate COX analyses indicated that dyslipidemia was significantly associated with HBsAg clearance and was an independent predictor of HBsAg clearance (HR = 0.243, P = 0.001). Kaplan-Meier survival analyses show that cumulative HBsAg clearance was significantly higher in the normolipidemic group than in the dyslipidemia group (log-rank test, P = 0.007). During the treatment, triglyceride showed an increasing trend, while the levels of total cholesterol, high-density lipoprotein, low-density lipoprotein, apolipoprotein A1 and apolipoprotein B decreased. Dyslipidemia and other indicators independently associated with HBsAg clearance were used to construct the nomogram model. The AUC of the model at 36-week and 48-week were 0.879 and 0.856, and the model demonstrated good discrimination and calibration., Conclusion: Dyslipidemia can affect the antiviral efficacy of PegIFNα in NAs-experienced CHB patients. Our findings suggest that the nomogram model constructed using serum lipid has good predictive power and may help physicians to identify the superior patients for PegIFNα therapy., Competing Interests: Declarations Ethics approval and consent to participate The study protocol was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of Biomedical Research Affiliated to Fujian Medical University (No. 2014-87), who waived the need for informed consent. Consent for publication Not applicable. Competing interests The authors declare no competing interests., (© 2024. The Author(s).)

Introduction: Cataracts are a significant cause of blindness. While individuals frequently turn to the Internet for medical advice, distinguishing reliable information can be challenging. Large language models (LLMs) have attracted attention for generating accurate, human-like responses that may be used for medical consultation. However, a comprehensive assessment of LLMs' accuracy within specific medical domains is still lacking., Methods: We compiled 46 commonly inquired questions related to cataract care, categorized into six domains. Each question was presented to the LLMs, and three consultant-level ophthalmologists independently assessed the accuracy of their responses on a three-point scale (poor, borderline, good) and their comprehensiveness on a five-point scale. A majority consensus approach established the final rating for each response. Responses rated as 'Poor' were prompted for self-correction and reassessed., Results: For accuracy, ChatGPT-4o and Google Bard both achieved average sum scores of 8.7 (out of 9), followed by ChatGPT-3.5, Bing Chat, Llama 2, and Wenxin Yiyan. In consensus-based ratings, ChatGPT-4o outperformed Google Bard in the 'Good' rating. For completeness, ChatGPT-4o had the highest average sum score of 13.22 (out of 15), followed by Google Bard, ChatGPT-3.5, Llama 2, Bing Chat, and Wenxin Yiyan. Detailed performance data reveal nuanced differences in model capabilities. In the 'Prevention' domain, apart from Wenxin Yiyan, all other models were rated as 'Good'. All models showed improvement in self-correction. Bard and Bing improved 1/1 from 'Poor' to better, Llama improved 3/4, and Wenxin Yiyan improved 4/5., Conclusions: Our findings emphasize the potential of LLMs, particularly ChatGPT-4o, to deliver accurate and comprehensive responses to cataract-related queries, especially in prevention, indicating potential for medical consultation. Continuous efforts to enhance LLMs' accuracy through ongoing strategies and evaluations are essential., (© 2024. The Author(s).)

Beef quality is a critical factor in evaluating the effectiveness of beef cattle production. Fiber types play key roles in determining muscle growth and meat quality characteristics. FHL3 is de novo expressed in skeletal muscle and is responsible for MyHC isoform expression in C2C12 cells. Nevertheless, the precise function of this factor in regulating the proliferation, differentiation, and fiber type of bovine skeletal muscle cells (BSMCs) have yet to be identified. This study aimed to investigate the impact of the FHL3 on BSMCs proliferation, differentiation, and muscle fiber types. The results revealed that the FHL3 promoted BSMCs proliferation, inhibited differentiation, increased type II muscle fiber expression, and decreased type I muscle fiber expression. Meanwhile, the FHL3 promoted the expression and phosphorylation levels of PI3K, Akt, and mTOR in the PI3K/Akt/mTOR signaling pathway, and inhibited the expression and phosphorylation levels of PI3K, Akt, and mTOR after treatment with the pathway inhibitor LY294002, furthermore, it promoted differentiation and inhibited proliferation of BSMCs, while promoting the expression of type II muscle fibers and inhibiting the expression of type I muscle fibers. The results suggest that the FHL3 has an effect on promoting the proliferation and inhibiting the differentiation of BSMCs through the PI3K/Akt/mTOR signaling pathway, but the effect of the FHL3 on myofiber type conversion is not regulated by this pathway. The objective of this study is to enhance our understanding of the molecular function of FHL3 in the development of BSMCs., Competing Interests: Declaration of competing interest The authors declare that this study was conducted without any business or financial relationship that could be considered a potential conflict of interest., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Metabolic syndrome is a prevalent non-communicable disease characterized by central obesity, insulin resistance, hypertension, hyperglycemia, and hyperlipidemia. Epidemiological statistics indicate that one-third of the world's population is affected by metabolic syndrome. Unfortunately, owing to complicated pathogenesis and limited pharmacological options, the growing prevalence of metabolic syndrome threatens human health worldwide. Autophagy is an intracellular degradation mechanism that involves the degradation of unfolded or aggregated proteins and damaged cellular organelles, thereby maintaining metabolic homeostasis. Increasing evidence indicates that dysfunctional autophagy is closely associated with the development of metabolic syndrome, making it an attractive therapeutic target. Furthermore, a growing number of plant-derived polysaccharides have been shown to regulate autophagy, thereby alleviating metabolic syndrome, such as Astragalus polysaccharides, Laminaria japonica polysaccharides, Ganoderma lucidum polysaccharides and Lycium barbarum polysaccharides. In this review, we summarize recent advances in the discovery of autophagy modulators of plant polysaccharides for the treatment of metabolic syndrome, with the aim of providing precursor compounds for the development of new therapeutic agents. Additionally, we look forward to seeing more diseases being treated with plant polysaccharides by regulating autophagy, as well as the discovery of more intricate mechanisms that govern autophagy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Hydrocephalus is a cerebrospinal fluid-related disease that usually manifests as abnormal dilation of the ventricles, with a triad of clinical findings including walking difficulty, reduced attention span, and urinary frequency or incontinence. The onset of congenital hydrocephalus is closely related to mutations in genes that regulate brain development. Currently, our understanding of the mechanisms of congenital hydrocephalus remains limited, and the prognosis of existing treatments is unsatisfactory. Additionally, there are no suitable or dedicated model organisms for congenital hydrocephalus. Therefore, it is significant to determine the mechanism and develop special animal models of congenital hydrocephalus. Recently, zebrafish have emerged as a popular model organism in many fields, including developmental biology, genetics, and toxicology. Its genome shares high similarity with that of humans, and it has fast and low-cost reproduction. These advantages make it suitable for studying the pathogenesis and therapeutic approaches for various diseases, specifically congenital diseases. This study explored the possibility of using zebrafish as a model organism for congenital hydrocephalus. This review describes the characteristics of zebrafish and discusses specific congenital hydrocephalus models. The advantages and limitations of using zebrafish for hydrocephalus research are highlighted, and insights for further model development are provided.

Patients with 22q11.2 deletion syndrome or DiGeorge syndrome commonly report gastrointestinal symptoms in addition to more widely understood cardiac and immunodeficiency abnormalities. However, the morphologic features of gastrointestinal tract pathology in these patients are poorly understood. We previously reported that plasma cells are essentially absent from the luminal gastrointestinal tract of patients with "complete" DiGeorge syndrome. Herein, we add to the current understanding of the luminal gastrointestinal tract changes in patients with DiGeorge syndrome. Patients with cytogenetically confirmed DiGeorge syndrome were identified after approval from our institutional review board. Gastrointestinal tract biopsies from patients with DiGeorge syndrome that were severely immunosuppressed (complete DiGeorge syndrome, DGS-I), partially immunocompromised (partial DiGeorge syndrome, DGS), and from control patients were reviewed. Two panels of chromogenic multiplex immunohistochemistry (IHC) were performed to evaluate the immune cell infiltrate in the lamina propria of the duodenum and colon. "Panel #1" was composed of antibodies targeting CD3, CD20, and CD68. "Panel #2" was composed of antibodies targeting CD4, CD8, CD56, and TCRϒδ. Assessment of cell types identified by these antibody targets demonstrated a significant reduction of duodenal and colonic T-cells in patients with complete DiGeorge syndrome. In addition to establishing the morphologic phenotype of the luminal gastrointestinal tract of patients with DiGeorge syndrome, we also highlight our chosen technology of chromogenic multiplex IHC as a relatively accessible research and diagnostic tool with wide potential to be utilized across various disease processes., Competing Interests: Declaration of competing interest None., (Copyright © 2024 Elsevier Inc. All rights reserved.)

The recent advances in micromanufacturing have been pushing boundaries of the new generation of semiconductor devices, which, in the meantime, brings new challenges in the material and structural characterization - a key step to ensure the device quality through the micromanufacturing process. An ultrafast laser-enable optoacoustic characterization methodology is developed, targeting in situ calibration and delineation of the three-dimensional (3-D), nanoscopic interior features of opaque semiconductor chips. With the guidance of ultrafast electron-phonon coupling effect and velocity-perturbated optical interference, a femtosecond-laser pump-probe set-up based on Sagnac interferometer is configured to generate and acquire picosecond ultrasonic bulk waves (P-UBWs) traversing the microchips. The interior features of the microchips shift the phase of acquired P-UBW signals, reflected in the perturbed probe laser beam. The phase shifts are calibrated to compute signal correlation of P-UBW signals between different acquiring positions, whereby to delineate the interior features in an intuitive manner. The approach is experimentally validated by characterizing nanoscopic, invisible interior aurum(Au)-gratings with periodically varied depths in typical microchips. Results highlight that the 3-D nanoscopic features of the microchips can be revealed with a microscopic and a nanoscopic spatial resolution, respectively along the transverse and depth directions of the chip, where the Au-gratings become "visible" with a depth variance of a few tens of nanometers only. This proposed approach has provided a fast, nondestructive approach to "see" through an opaque microchip with a nanoscopic resolution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: Nesodiprion zhejiangensis, a multivoltine sawfly, is widely distributed in south China and has caused serious damage to forests. Historically, N. zhejiangensis management has relied heavily on synthetic chemicals. To reduce the reliance on chemical control, we previously isolated a nucleopolyhedrovirus, NezhNPV, from deceased N. zhejiangensis larvae. A subsequent pathogenicity assay confirmed its high virulence in a laboratory setting., Results: In order to comprehensively examine the hypothesis that NezhNPV is an effective new biocontrol agent for N. zhejiangensis, we carried out a field test in Beijing, China, and characterized NezhNPV morphologically by electron microscopy and genetically by genome sequencing. Our field trials showed that NezhNPV was effective in controlling N. zhejiangensis in a naturally infested Himalayan blue pine forest. The occlusion bodies of NezhNPV consist of irregular polyhedra that occlude rod-shaped enveloped virions with a single nucleocapsid per virion. The NezhNPV genome is 80 637 bp in length, and contains 90 open reading frames, including 38 core, eight lepidopteran baculovirus, 34 hymenopteran baculovirus and 10 unique baculovirus genes, representing the smallest known genome among baculoviruses. The combined results based on phylogenetic analyses, Kimura-2-parameter distances and biological characteristics indicate that NezhNPV is a novel gammabaculovirus and candidate for species status with the provisional name Gammabaculovirus nezhejiangensis. NezhNPV is highly collinear with other gammabaculoviruses and contains nonsyntenic regions with an inversion and rearrangement between orf3 and orf35., Conclusion: The combined results from our field trials, coupled with morphological and genomic characterization clearly demonstrate the bioactivity of NezhNPV. This gammabaculovirus may be included in pest management practices against N. zhejiangensis as a novel biocontrol agent. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry., (© 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.)

Self-assembled monolayers (SAMs) are employed as hole-selective contacts in inverted perovskite solar cells (PSCs) and have achieved record power conversion efficiency (PCE) over 26%. However, the tendency of extensively employed SAM [2-(3,6-Dimethoxy-9H-carbazol-9-yl)ethyl]phosphonic acid to aggregate leads to its uneven coverage to the transparent conducting oxide substrate, which subsequently compromises the photovoltaic performance. Herein, a novel tert-butyl functionalized phosphonic acid carbazole SAM is developed, i.e., (4-(3,6-di-tert-butyl-9H-carbazol-9-yl)butyl)phosphonic acid (tBu-4PACz), and introduced to a mixed SAM system as the hole-extraction layer in inverted PSCs. The stereo-hindrance of the bulky tert-butyl group prevents undesired aggregation and leads to better conformality, which facilitates more efficient hole-extraction and suppresses interfacial recombination losses. The tBu-4PACz SAM-based inverted PSC has achieved record level PCE of 26.25% (26.21%, certificated) with outstanding fill factors over 86%. Moreover, the mixed SAM based inverted PSC devices maintained over 94.7% of their initial efficiency after 500 h continuous maximum power-point tracking under simulation 1-sun irradiation., (© 2024 Wiley‐VCH GmbH.)

Escherichia coli is a commonly used platform for the production of heterologous proteins. Extraction and purification of intracellularly expressed recombinant proteins rely on efficient cell disruption. To facilitate downstream processing, controlled autolytic cells have been designed that lyse automatically to release intracellular proteins when triggered with an internal or external signal. In the cases when a weak promoter has to be adopted to control autolysis, cell lysis and product release progress slowly even in the presence of surfactants or other adjuvants. In this study, we report an improved autolytic E. coli strain controlled by a weak promoter with higher efficiency without the use of any facilitating chemical. Cell lysis was initiated upon arabinose-induced expression of T4 lysozyme with N-terminal fusion of amphipathic cell-penetrating peptides via a flexible peptide linker. Furthermore, genes involved in membrane permeability were individually deleted and screened for leaky phenotypes. Deletion of lpp (encoding Braun's lipoprotein) combined with the autolytic system caused 96% cell lysis in 4 h of induction and released 84% or 67% of mCherry or a super large Cas13a fusion protein (160.8 kDa), respectively, in 10 h of induction. This autolytic leaky strain shows great promise for protein recovery and library screening.

The exceptional selectivity and responsive ion transport in biological channels inspire technology breakthrough in energy, environmental, and resource sectors. However, existing nanofluidic systems with a high photothermal conversion efficiency often exhibit excessive thermal conductivity, which impedes the creation of effective temperature gradients and results in a low ion transport efficiency. In this study, a strategy based on the synchronic stacking of metallic and nonmetallic two-dimensional (2D) nanosheets was presented to construct heterogeneous nanofluidic channels. This specific nanoconfined architecture sustained high temperatures in the illuminated area while maintaining low temperatures in the nonilluminated area, thus obtaining a robust driving force from sunlight for directional ion transport. As a result, our light-responsive ion transport system demonstrated significant potential in solar energy conversion and osmotic energy harvesting, surpassing those of all previously reported nanofluidic systems. Additionally, although it is still at the proof-of-concept stage, it shows great promise in light signal monitoring. This work provides an effective strategy for developing advanced light-responsive ion transport systems and their important applications.

Owing to the co-occurrence of hazardous compounds, it is crucial to build multiple highly discriminative probe libraries for simultaneous determination. Drawing inspiration from nucleic acid barcodes, we developed a probe system that is exclusively based on the nucleic acid secondary structure's hairpin structure, which can be directly read by nanopores. The highly distinguishable hairpin probes were constructed, and a detailed explanation of the possible patterns in their design was provided. These probe-representative events measured through the α-hemolysin (α-HL) nanopores were both distinguished, either through visual observation or comparison of the nanopore parameters. Besides, the potential design pattern for probes with unique telegraphic switching between the two levels was also unveiled. Finally, these probes were utilized to realize simultaneous, ultrasensitive mycotoxin multiple-detection, and their prospective applications for the detection of proteins and microRNAs were presented, indicating their suitability for a wide range of sensing applications.

As a kind of commonly-used synthetic materials for many pesticides, thiol compounds, once being leaked, can cause serious harm to the environment and humans. Therefore, the efficient detection of thiol compounds is essential. In this study developed a turn-on fluorescent probe (Cu@Zn-CP) for the highly sensitive fluorescence detection of thiol compounds. The probe was constructed based on a zinc coordination polymer (Zn-CP), whose fluorescence was quenched through the effective doping of Cu 2+ ions. After the introduction of methyl thioglycolate (MTC), a rapid fluorescence turn-on response was generated within 90 s with a low detection limit of 23 ppb. Even after being reused for five cycles, the sensor maintains excellent detection performance and demonstrates good recyclability. It can also detect MTC in river water, with a spike recovery rate between 98-103 %. Furthermore, the designed Cu@Zn-CP exhibits good universality for detecting multifarious thiol compounds, including L-cysteine, glutathione, monothioglycerol, and 2-hydroxy-1-ethanethiol. This result provides a potential recyclable fluorescent sensor for thiol compounds., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Conventional chemotherapy, especially with natural anticancer drugs, usually suffers from poor bioavailability and low tumor accumulation. To address these limitations, we developed a novel approach for modifying natural products in which amphiphilic hydroxamic acid hybrids based on a natural product: isoalantolactone (IAL) were rationally designed. Compound 18 is identified as a highly potent dual signal transducer and activator of transcription 3 (STAT3)/histone deacetylases (HDAC) inhibitor and induces autophagy and apoptosis. 18 exhibits higher antitumor potency than IAL and the hydroxamic acid SAHA in vitro and in vivo. Furthermore, 18 self-assembled in water to form nanoparticles (18 NPs), which facilitated the accumulation of drugs in tumor tissues and promoted their cellular uptake, resulting in superior anticancer efficacy compared to free 18. Compared to drug-drug conjugates, hydroxamic acid hybrids have a smaller molecular weight and can synergize with various anticancer drugs. Overall, these findings indicate that 18 utilizing nanomedicines and dual-target drugs provide an efficient strategy for the rational design of dual-target drugs and the modification of natural products., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Bipolar plates are one of the most important components of proton exchange membrane fuel cells. With the miniaturization of bipolar plate flow channel sizes and the increasing demand for precision, springback has become a key focus of research in the bipolar plate forming process. In this paper, the hydroforming process for 316L stainless steel bipolar plates was studied, and an FEM model was built to examine the stress and strain at various locations on the longitudinal section of the plate. Modeling accuracy was validated by the comparison of experimental profile and thickness distribution. The effects of forming pressure and grain size on springback behavior are discussed. The results show that with increasing forming pressure, the springback value decreases initially, followed by an increase, but then again decreases. When the forming pressure is 80 MPa-100 MPa, the deformation of the lower element of the upper rounded corner is not uniform with more elastic regions, and the springback is positively correlated with forming pressure. The springback distribution pattern on the cross-section of the bipolar plate changes from a normal distribution to a distribution of "M" shape with increased pressure. The larger the grain size, the lower the yield strength elastic proportion, resulting in a decrease in springback of the sheet. The maximum amount of springback of the bipolar plate is 3.1 μm when the grain size is 60.7 μm. The research results provide a reference for improving the forming quality of metal bipolar plates with different flow channel shapes.

Purpose: To evaluate the performance of two non-disease-specific patient-reported outcome (PRO) instruments, the National Eye Institute Visual Function Questionnaire-25 (VFQ-25) and the Low Luminance Questionnaire (LLQ), in patients with retinaldehyde-binding protein 1 retinal dystrophy (RLBP1 RD)., Methods: PROs were assessed using the VFQ-25 and LLQ. Rasch analysis was conducted to estimate person and item measures of the VFQ-25 and LLQ questionnaires to determine the association between the two PROs. In addition, the association between these two instruments and their correlations to weighted measures of visual function and disease progression were analyzed in this three-year PRO-focused sub-study of a five-year prospective natural history study., Results: Forty-two patients participated, with most of them having completed at least two PRO follow-up visits at least one year apart. The mean VFQ-25 scores were lowest for distance activities (39.2-49.0) and peripheral vision (37.5-52.4), with mean LLQ subscale scores generally low (<41), except for the emotional distress subscale. Using Rasch analysis, calibrated item and person measures along with their standard errors were estimated for both ePROs. This indicated that the distribution of the VFQ-25 and LLQ item measures well covered the distribution of person function in this group. This suggests that the item difficulties well cover the person-level performance in this population. As well, the two PROs showed a strong and significant correlation at all assessed time points as assessed with Pearson correlation coefficient (0.81, 0.91, 0.81 and 0.87 at baseline, 1/1.5, 2/2.5 (P < 0.001) and 3/3.5 years (P = 0.002)). The composite scores of both PRO questionnaires strongly correlated with clinical measures of visual function. At 2 to 2.5 years of follow-up, meaningful statistically significant declines in peripheral vision (both VFQ-25 and LLQ), distance vision (VFQ-25), and extreme lighting in dark and bright light (LLQ) subscales were noted., Conclusions: This study demonstrated a strong association between VFQ-25 and LLQ scores and their association with clinical measures of visual function., Translational Relevance: PRO instruments can provide insights into the specific disabilities of this unique patient population and help to guide appropriate outcome measures for future clinical trials.