1. Force-mediated recruitment and reprogramming of healthy endothelial cells drive vascular lesion growth.
- Author
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Shapeti A, Barrasa-Fano J, Abdel Fattah AR, de Jong J, Sanz-Herrera JA, Pezet M, Assou S, de Vet E, Elahi SA, Ranga A, Faurobert E, and Van Oosterwyck H
- Subjects
- Humans, Human Umbilical Vein Endothelial Cells metabolism, Extracellular Matrix metabolism, Integrin beta1 metabolism, Integrin beta1 genetics, Actin Cytoskeleton metabolism, Cellular Reprogramming genetics, Cell Proliferation, Mutation, rho-Associated Kinases metabolism, rho-Associated Kinases genetics, Animals, Endothelial Cells metabolism, Endothelial Cells pathology, Hemangioma, Cavernous, Central Nervous System pathology, Hemangioma, Cavernous, Central Nervous System metabolism, Hemangioma, Cavernous, Central Nervous System genetics, Neovascularization, Pathologic genetics, Neovascularization, Pathologic pathology, Neovascularization, Pathologic metabolism
- Abstract
Force-driven cellular interactions are crucial for cancer cell invasion but remain underexplored in vascular abnormalities. Cerebral cavernous malformations (CCM), a vascular abnormality characterized by leaky vessels, involves CCM mutant cells recruiting wild-type endothelial cells to form and expand mosaic lesions. The mechanisms behind this recruitment remain poorly understood. Here, we use an in-vitro model of angiogenic invasion with traction force microscopy to reveal that hyper-angiogenic Ccm2-silenced endothelial cells enhance angiogenic invasion of neighboring wild-type cells through force and extracellular matrix-guided mechanisms. We demonstrate that mechanically hyperactive CCM2-silenced tips guide wild-type cells by transmitting pulling forces and by creating paths in the matrix, in a ROCKs-dependent manner. This is associated with reinforcement of β1 integrin and actin cytoskeleton in wild-type cells. Further, wild-type cells are reprogrammed into stalk cells and activate matrisome and DNA replication programs, thereby initiating proliferation. Our findings reveal how CCM2 mutants hijack wild-type cell functions to fuel lesion growth, providing insight into the etiology of vascular malformations. By integrating biophysical and molecular techniques, we offer tools for studying cell mechanics in tissue heterogeneity and disease progression., (© 2024. The Author(s).)
- Published
- 2024
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