1. Biorefinery approach with green solvents for the valorization of Citrus reticulata leaves to obtain antioxidant and anticholinergic extracts.
- Author
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Domínguez-Rodríguez G, Amador-Luna VM, Benešová K, Pernica M, Parada-Alfonso F, and Ibáñez E
- Subjects
- Green Chemistry Technology, Solvents chemistry, Chromatography, Supercritical Fluid methods, Deep Eutectic Solvents chemistry, Phenols chemistry, Phenols isolation & purification, Citrus chemistry, Plant Leaves chemistry, Plant Extracts chemistry, Plant Extracts isolation & purification, Antioxidants chemistry, Antioxidants isolation & purification
- Abstract
Citrus reticulata L leaves are one of the main post-harvest byproduct, containing bioactive compounds, that are usually undervalued. This work describes the development of a biorefinery process based on the application of supercritical CO
2 (SC-CO2 ) followed by ultrasonic-assisted extraction (UAE) combined with Natural Deep Eutectic Solvents (NaDES) to extract bioactive terpenoids and phenolic compounds from these leaves. Extraction temperature and pressure of SC-CO2 were optimized, obtaining the highest bioactive terpenoids content using 200 bar at 60 °C. A Box-Behnken experimental design showed that 57% of water in NaDES composed of Choline Chloride and Glycerol (1:2) as extraction solvent at 25 °C for 50 min were the optimal UAE-NaDES extraction conditions to obtain the highest bioactive phenolic content from the residue of the optimal SC-CO2 extraction. The optimum extract presented the highest bioactivity and polyphenol content determined by LC-DAD-MS compared with extracts obtained using only water or NaDES as solvent., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)- Published
- 2024
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