1. The influences of extraction methods on the chemical constituents of Lyonia ovalifolia (wall.) Drude and intracellular protective effects based on metabolomics analysis.
- Author
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Li C, Li M, Zhao Z, Khan A, Zhao T, Liu Y, Wang Z, and Cheng G
- Subjects
- Apoptosis drug effects, Animals, Superoxide Dismutase metabolism, Reactive Oxygen Species metabolism, Flavonoids chemistry, Flavonoids isolation & purification, Flavonoids analysis, Protective Agents pharmacology, Protective Agents chemistry, Protective Agents isolation & purification, Mice, Nitric Oxide metabolism, Humans, Catalase metabolism, Antioxidants chemistry, Antioxidants isolation & purification, Antioxidants pharmacology, Metabolomics, Plant Extracts chemistry, Plant Extracts isolation & purification, Plant Extracts pharmacology
- Abstract
Lyonia ovalifolia (Wall.) Drude (LO) is mainly distributed in China with health benefits. In this study, LO buds (LOB) were extracted by ultrasonic extraction (UE) with or without ultra-high-pressure (UHP-UE), microwave (MW-UE), subcritical (SC-UE) techniques. The metabolomic result showed that a total of 960 chemical compounds and 117 differential compounds were identified from LOB extracts. The UHP-UE extract was rich in total polyphenol and flavonoid contents, followed by MW-UE, UE and SC-UE extracts, respectively. All LOB extracts increased superoxide dismutase (SOD) and catalase (CAT) activities, and glutathione (GSH) content, decreased reactive oxygen species (ROS) accumulation, levels of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor -α (TNF-α), and nitric oxide (NO), and alleviated apoptosis in cells. The cellular protective effect was UHP-UE > MW-UE > UE > SC-UE. This study revealed that higher pressure and lower temperature may be key factors for increasing bioactivities of LOB extracts., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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